LOCUS       DAORVT010000001        15970 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_1596_length_15970_cov_4.824694, whole genome shotgun sequence.
ACCESSION   DAORVT010000001 DAORVT010000000
VERSION     DAORVT010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 15970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15970
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1596_length_15970_cov_4.824694"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..559
                     /locus_tag="PK389_00005"
     CDS             116..559
                     /locus_tag="PK389_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015208257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF29 domain-containing protein"
                     /protein_id="HQY22127.1"
                     /translation="MGHLNHHSYEKDFYAWAMENAELLKQKKFEEIDIDNIAEEIESM
                     GKSNKRSLFSHLSVLIAHLLKWEFQPIRRSKSWTLTIENQRFEITDLLQESPSLKPEI
                     ELQFDHAYKKALIIASEQTGIDKKDFPKFCPFNLEQCLNNDFLPD"
     gene            complement(779..4546)
                     /locus_tag="PK389_00010"
     CDS             complement(779..4546)
                     /locus_tag="PK389_00010"
                     /inference="COORDINATES: protein motif:HMM:NF014901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HQY22128.1"
                     /translation="MNLIEAIKQNNKKEVFRLLDKKTDLFGKKFIDEVDSECEGSPLY
                     WAAANGYGHFIGPLVKAGADVNKLDKYGRTPVFIAAQNGHAAAITQLKYAGADVDIPN
                     SNGITPVYAAAQFRYAADVIRELRIAGANVNTPDRDGRTPVFIAAQNGHAAAITQLKY
                     AGADVDIPNSNGITPVYVAVENRHVETVAALLEAGADAGVGPSWEEKTTPLEQARKKG
                     YEEIVNLLKKHFENKRIKSSANLHQQSNLSGSYQVDWERTDAWRDLSEPMSSVEAIKN
                     NNQKEFFRLLNKAFVLTVSIVDLVQAGIDVNKKNDGGETSVCIAAAKGHADVIRALKA
                     AGANVNTPNANGETPVCIAAVKGHADVIRALKAAGANVNTQNKNYESPLHIAAKNGDI
                     DVIKALTISGANLNVIDQNHKKPYEHAKGQEAIDLLTPVHKNTYTSDNIFEEEEQTTV
                     SFHTTSSPPVVNDFNYPCLSREGFVLGGVVDIPGRVAEEPDLDGIKSIKKGFQSPHVK
                     QKKSSNVIHYQGLKADRSRLKINSAYQYEDNDIQVILSARLQQLQIQNKQLFTKPLAV
                     LAAVDNIVGTQLEHRLKQEQEANNHRCACVLLIPCNLRNAHWVGILLELRIDRTISRA
                     EYVDSNDSHPTVPSTFQEQLQNVYPNAPLITVRKSLQQTDGSSCGAYTIENLLTAALG
                     IQSPENSEIIRRLHLEALEQYNLGFYSAFYERQKNNRPTTVTLHQQLGYVNKLKDIWF
                     SKPELNRILMIKRCVSSLPEKIQSDLLQAFEYKSTYEDDHAAHLNTIRKALKEAAQHE
                     SKSLVELIQLLFDQDWQPGMPLPLEQKFRVDYNEILAITKTNLAVEKVNSLQQSLAEK
                     IQKDEQFALKLQAELWSDTVASNVDQNEVAKFLKLVAEGEQVKAEEMLNDNCDLALIP
                     GDVTDLSNRTFRGITGFQYSIWALDWHMWTMLLKYMSFASTAKQAQSMETGIWVKEHG
                     LSASWKDLIDAIHTYIDLCNRSSLKKVTTQWKTQVGTAQRLLPAHVVNEYCRPDRPFE
                     PCPDFTTPCLPRTRDTKQGEWFTATCDNEGLGTTFAYFRYKNESARPMLAGHDNANLG
                     RRAPGRVMSDYRACETLLTTRLQQRNELFIEQLIMPILRTFINIPSYGPIDIILDYLG
                     MNTVVQKQHQSSGDSNHSPSTSHPVLTNLQNRPQPSRKFDDSLFLRPVKAQRGVALGN
                     GRVEANVNQPPPPPMLHAFLPANPINSQSFVRSADQQQVERPHNRSESPRR"
     gene            complement(4783..5073)
                     /locus_tag="PK389_00015"
     CDS             complement(4783..5073)
                     /locus_tag="PK389_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HQY22129.1"
                     /translation="MRKKPLTNKIGEVRELTREDLRAMRPAKEVLPDSLLTLLPRRKV
                     GQRGQQKQPTKVPVTLRYSPEVVKYFKTTGKGWQIRMDEALKEWIKNHPHAA"
     gene            complement(5048..5326)
                     /locus_tag="PK389_00020"
     CDS             complement(5048..5326)
                     /locus_tag="PK389_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020483566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HQY22130.1"
                     /translation="MKIDFDPEKSNKNIRERGLAFELIVEFDWETAIYAEDIRSLYAE
                     RRFITIGYIGQRLHVVCFTPIKGGIRAISFRKANLREVKRYEKETINQ"
     gene            5523..6161
                     /locus_tag="PK389_00025"
     CDS             5523..6161
                     /locus_tag="PK389_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011733804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HQY22131.1"
                     /translation="MLMGYIRVSKQDGSQQTDLQKDALLSVGVDVANIYQDYASGKID
                     ARPGLLSVLKALRAGDTLVVWKLDRLGRNLRHLINTVLELVERGIGFKVLTGHGASID
                     TTTPAGKLVFAIFAALAEFERELISERTKAGLASARARGHQGGAPFKMTAAKVRLSMV
                     AMGKPETKVGDLCRELKVSRQTLYRHVDPNGALRKDGKKLLKSKKTNLIYLT"
     gene            complement(6298..9801)
                     /locus_tag="PK389_00030"
     CDS             complement(6298..9801)
                     /locus_tag="PK389_00030"
                     /inference="COORDINATES: protein motif:HMM:NF016451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HQY22132.1"
                     /translation="MPKDNIIPEKYRCSITGQIMMEPVFTSDGHTYEREKIDEWLQTH
                     NTSPKTGVNLENKNLTVNHDKRSDISDFLENNPELYDGDEVYLPKAWVEKCVMVIKQN
                     QLQVVHQWLNKDRRLLTLKLEGNYTALHLACEFSSPELVEMLLNILKQKNNFIFTLDG
                     IRLNPKHLNVLLEKALNLNRNDYVECELLLSLGAEVEQPESDTQNTLLHRMVIKDNKD
                     NLEESIKWLLERKAALESRNIEGNTSLMLSVIQGNIRLTELLLKMKADIKTKNNQQQT
                     ALHIAASRGDVKMLEYLLQNTEVALALIDVQNAKGDTPLHLAVQFGQDNVISLLLEAG
                     AYHKIKNEQGKTPIDLSIEQKKPNFIVQTVRRLKKDKLKETDRLHQVFVEQVSEIATL
                     KTALQNQEQGFKTQIFYIQQTMEQERIKNEKLIADMQAHIKLLQPAVQEPNQSSPTAL
                     TFSPQASPCSVRIHASLSVPYVNPNDIAEFLTFVAEGEQGKAKAMLKSNRDLALVSGN
                     VIDLSNRIFNGITGFQYAVWALDWHMWTMIKQYLPNEAAREQAQGFEKGFWVKQHGVH
                     ANLNNLIQAYQKTIHLYNAKKYTDGNTAWVQEVGGEQRLLPAHVVNEYCHPIRPFYPV
                     PNFKDADVALPRTRTIETGEDWFYDENLGKQFGVYRSAQPVSRVRKKVGGTLGWRWGE
                     ESAELRDHQSIIALASTRIDQREELITELRTGLRCISLISTAEISKMINSSVNILSKP
                     TETSKTTINISYQYEDNDIQAIVSARLQQLRIQNSQLFIKPIEILAAIDNIMSVQLEN
                     RLRQEARNHHGERICLIPCNLGNAHWVGMLLEFSADGKVLRAEYIDSFTTRPIIPEIF
                     QVQLQKIYPKVYFKTRVLSQQSDYTSCGAYTIENLLTAALGIQSYEAAEIIRSLHLEA
                     LRQYNPNFHNAFNERQRNNRPSTATLHEQLGYLYESNPIRFSTQELNRIFSIKQHLFI
                     LPEKIQIGLLEAFKHNSAYNDDHALHLNTIRTALQEAAQVDSKALKELMQLLFENWQA
                     EDSATLDKMKFRVSYNEILAVTKRNLAQNEVSKLQKSLAEQIQKDEQLARELQAEWNC
                     DETFSLGNNVSSIETVEARQNSDEPPVNSPVVVHLANHNSFEQNNPLILTQFNQSDPS
                     TPEALSNQERQPLNQGTRYGH"
     gene            complement(10222..11574)
                     /locus_tag="PK389_00035"
     CDS             complement(10222..11574)
                     /locus_tag="PK389_00035"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HQY22133.1"
                     /translation="MLKSLVIENTPQKPIVSATPQSTVNDPVPRNTPQAALKLNPSEQ
                     KNTPILTQFQDFESSSVSISDLKSITSLIEAIKKNNQEEFFRLLKLKTDRFGKRFIDE
                     VDSEYNGSPLYWAAAFGHIHFIDPLLQAGADINKQDKQGRTLVYKAALNGHASAITAL
                     HTAGANVDTPNRNGATPVYIAAEKEHESAITALHAAGANVNMPQKDGATPAYVAAYNG
                     HASAITALYIAGANVNTSKDGGFTPVYIAAYNGHVSAIAALHAAGANVNTPISDGKTP
                     VFIAAYNGHASAITALYAAGANVDTPCDGATPVFIAAQNGHVSAIAALHAAGANMNTP
                     MPDGITSVYIAVCNGHTLAITALLEAGANASIKTKWGTALSFARQGKDPEHREAVKIL
                     EAHFKQYPNGIKPVRVMNPVVSFSAKPSSVLEQKDPPPVPKKPASQLSQAFRKAFGKG
                     "
     gene            11783..12037
                     /locus_tag="PK389_00040"
     CDS             11783..12037
                     /locus_tag="PK389_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22134.1"
                     /translation="MGSNPQPRRGLFVRKMERDKPSAHHRLDVQKRTPFAISTSLERS
                     SELSEDLCQAFAKTAALQEEMICLMAVLSLRIIHSKGAKK"
     gene            12034..13932
                     /locus_tag="PK389_00045"
     CDS             12034..13932
                     /locus_tag="PK389_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HQY22135.1"
                     /translation="MTHSLSKISDHHRQRRACVYIRQSTTFQVIHNRESTERQYNLRQ
                     RAIDLGWNENLIDIIDNDQGQSAKSIEHREGFQKLASEIVAKQVGIVLMLEVSRLSRS
                     CSDWHRLIEICSITRTLIADEAGIYDPREPNDRLLLGVKGTLSEAELFTLRTRLYEGR
                     WNKAKKGQLGRSIPTGYIKDEKGNWTKDPNLQVQERMNYIFKLFAQLGIARRVLLVLK
                     NENLKIPTKVWGGPQHGQLIWKEASLGSIMRLLKNPSYAGVYVYGACDYESNGYYAKT
                     GKAKPKARPLKEWTVCLQDHHEQYISFDEYMSNQDRLHQNWFRSKTRGAPREGRALLQ
                     GIVWCGQCGSKMGINSYSAKERRNSYTCTKDYQNGAQHVCQTISARTIDGLIVSLFLE
                     AMAPSQLEIAKKSIEKTQIEKQALKKQWEQQLTQARYDTQLAQRQYDAIDPDNRLVAS
                     ELERRWNEKLEILQKLEENYEEANKQSRFSLTLEEIKEMEMLIKDLDKIWYAATTTDR
                     ERKQLIRCAIAEVQLNGITIPQKVEIRITWRSGAVSEHIVDRIKQGIWAPRTSDIVIE
                     RIRILSEKYTVNKIAKLLNQEGHRSAHNRLFKDYHILYIARRNSIHVTTHLKGIKNPN
                     YRRKKLKD"
     gene            complement(13966..15207)
                     /locus_tag="PK389_00050"
     CDS             complement(13966..15207)
                     /locus_tag="PK389_00050"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQY22136.1"
                     /translation="MFPTVQILSENNDSPDWQPILDAFNQKPAENTEKGFLVSCQIEY
                     KDLNFGKELGQGSFGIVYQGSYQFGSVAIKKLQIGNLSPEAHEEFRKEADMMAQLRHP
                     NIVHFYGYCSIPRCLVMEYMPKGSLFSVLQDKTQALDWDVRIRIAIDMVSGLAFLHSK
                     SILHRDIKSLNILLDDQKAKLTDFGLSKVKNETKSLTKNHQKSKELVGTLPWIAPELF
                     EREQYTYKSDIYSLGITFWELASRKIPYAEDTPEAIPVFVGRGKREAIPSDCPEKLAH
                     LIQQCWAGNPESRPSASDIAKFMTTDAKTLEEASHRPTAAISISTSAHILDLNLQASP
                     AAVPAGRRGSGKLNLTGKEEQEKTQHVLEELRTFFIEERYIGEKTENYAIYNEYLKKD
                     NNNALLAYNSPNTYPTPNYIK"
     gene            complement(15510..>15970)
                     /locus_tag="PK389_00055"
     CDS             complement(15510..>15970)
                     /locus_tag="PK389_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22137.1"
                     /translation="ANLEDVLYQFFKLYDNWSLELKMVGQREAELLKTIEAFDEKIES
                     LDEVKEVIKESIQKEAKIALSVTVNTTATEVKKYCHAELDYLMQDLNRSLSYAKSVIP
                     EIQEFERNYSLRLWAIIVLGIFLIGFAAGTGWISHTTVSIDALKEKIRLK"
BASE COUNT         4584 a         3112 c         3081 g         5193 t
ORIGIN      
        1 tgaaagaaag tattgcaaaa cataagaaaa aaaatgaaat tgctaaagaa cttggtgtaa
       61 gccgagaaac ggtctatcga tatttaaaag cgaacgtata gcggagatta aaactatggg
      121 tcatcttaat catcattctt atgaaaaaga cttttatgcc tgggctatgg aaaatgcaga
      181 gttgctgaaa caaaagaaat ttgaggaaat cgatatcgat aatattgctg aggaaattga
      241 aagcatgggt aaaagtaata agagatctct tttcagtcat ctttctgttt taatagcgca
      301 tttactaaaa tgggagtttc aacctataag gcgcagcaaa agctggacgc ttactataga
      361 aaatcaacgg tttgaaataa ctgatttact acaagaaagt ccaagcttga aaccagagat
      421 agaattgcaa ttcgatcatg cgtataaaaa agcgctaatt attgcatccg aacaaacagg
      481 catagataaa aaagattttc ctaaattttg cccttttaac ttagagcaat gtcttaacaa
      541 tgatttttta ccagattaag tctattttta aaaattcttg ttaataaaac ataaaaacca
      601 attaaattgg atggtatttg tacacgttta acgccaacaa ttttacgcta ttcgttctga
      661 taaaacaatc tttttttatg gacacttccg tcaagctgcc aaataaagaa gctattaaat
      721 taacattttt taattattga ccccacacat ttttatgctc tccttcaggg ctatgtagtt
      781 accgtcgcgg actttcagat cggttatgcg gtctctctac ttgctgctga tcagctgatc
      841 gtacgaaaga ttgtgaatta atagggttag caggcaaaaa tgcatgaagc atagggggag
      901 gtggtggttg gttaacattt gcttctaccc ttccatttcc taacgccacc cctctttgtg
      961 cctttactgg tcttaaaaaa agactgtcat cgaatttcct tgaaggctgt ggtctatttt
     1021 gcaagtttgt aagtaccgga tggctggtac tagggctatg atttgaatcg cctgaagatt
     1081 gatgttgttt ttgaactaca gtattcatac ctaaataatc taaaattata tcaatcggcc
     1141 catatgatgg tatattgata aatgttctca atataggcat aatgagctgt tcgataaaca
     1201 gttcattacg ttgctgtaga cgggtggtca aaagtgtttc gcatgctctg tagtctgaca
     1261 tcacgcgacc tggcgcacga cgcccaagat tcgcgttgtc atgaccagca agcattgggc
     1321 gagctgattc attcttataa cgaaaataag caaacgtagt accaagccct tcattgtcgc
     1381 atgttgcagt aaaccactct ccctgtttcg tatcccgtgt gcgcggaaga caaggtgttg
     1441 taaagtctgg acaaggttca aaaggtctgt ccggtctgca atactcgttt acaacatgag
     1501 cgggtaataa gcgttgtgca gttcccactt gtgtcttcca ttgagttgtc acttttttta
     1561 aagacgatct attacataaa tcaatgtatg tgtggatggc atcaatgagg tccttccaag
     1621 aggcggaaag accgtgttcc ttaacccaga tcccagtttc catggattgt gcttgcttgg
     1681 ctgtagaagc aaaggacatg tatttaagta acattgtcca catgtgccag tcaagtgccc
     1741 atatcgaata ttgaaagcct gtgatgcccc tgaacgtacg gttggataaa tcggtaacat
     1801 cgccaggaat caatgctaaa tcgcaattgt catttagcat ctcttctgct ttgacttgtt
     1861 ctccttctgc aacaagcttc aaaaactttg ctacctcgtt ttgatctaca ttggacgcaa
     1921 cagtatcact ccataattct gcttgtaatt ttagtgcaaa ttgttcgtct ttctgtattt
     1981 tttctgctaa agattgctgt aaactgttaa ctttttccac tgcaagatta gtcttggtaa
     2041 tcgccagaat ttcattgtaa tcaaccctaa atttctgctc taacggcagt ggcatacctg
     2101 gctgccagtc ctggtcaaat aataattgta tcagctctac taaggattta gattcatgtt
     2161 gtgcggcttc tttcagagcc tttcgaatcg tatttaaatg cgcagcatgg tcatcctcgt
     2221 aagtagattt atattcaaaa gcttgcaata aatcgctttg gattttttct ggcaagctag
     2281 atacgcacct cttaatcatc agtattcgat tcagctctgg tttcgaaaac cagatatctt
     2341 ttaacttatt tacatatcca agctgctggt gaagtgtaac cgtggttggg cgattgtttt
     2401 tttggcgttc atagaaggct gaataaaaac ctaggttata ttgttccaaa gcttctaggt
     2461 gcaaacgacg aatgatttcg gaattttcag gtgattgaat acctaaagct gccgtcagta
     2521 agttctcaat agtgtatgca ccacagctag agccatctgt ttgttgtaat gacttacgta
     2581 ctgtaataag aggtgcgttt ggataaacat tttgcaattg ctcttggaat gtgcttggaa
     2641 cggtaggatg agagtcattt gaatccacat attcagcacg agagattgtt ctatctattc
     2701 tcaattctaa taatattcct acccaatgtg cattccttaa attacatggg ataagaagaa
     2761 cacatgcgca tcggtgattg tttgcttctt gttcttgttt taatctatgc tctagttgag
     2821 tacctacaat attatcaaca gcagccaata ctgccaaagg tttggtaaac agttgtttat
     2881 tttgtatctg tagctgctgt aagcgagcag ataaaatgac ttgaatgtca ttatcttcat
     2941 attgataggc agaatttatt tttagtctgc ttctatctgc tttaagccct tgataatgta
     3001 ttacgtttga ggattttttt tgtttaacgt gtgggctctg aaaccccttt tttattgact
     3061 taattccatc aaggtcaggt tcttctgcaa ctcttcctgg gatatcaaca acgccgccaa
     3121 gaacaaagcc ttcacgactt aaacaaggat aattaaaatc atttactact ggtggtgacg
     3181 aggtagtatg gaaagaaacc gtggtttgtt cctcctcttc aaatatgttg tccgaagtat
     3241 acgtattttt atgcacgggg gtcagtaagt ctattgcttc ctgtccttta gcatgttcat
     3301 aaggcttttt gtgattctga tctatcacat tcaaatttgc acctgaaatc gttagtgctt
     3361 ttattacatc tatatcgcca tttttagctg cgatatgaag tggactttcg taatttttat
     3421 tttgcgtatt tacatttgcg cctgccgctt ttagtgccct tatcacatct gcatgcccct
     3481 ttacagcagc aatacaaacc ggcgtttcgc catttgcatt tggcgtattt acatttgcgc
     3541 ctgccgcttt tagtgccctt atcacatctg catgcccctt tgcagcagca atacaaaccg
     3601 acgtttcgcc accatcattt ttcttattga catctattcc tgcttgcact aaatcaacta
     3661 tactaacagt taacacaaaa gccttattta aaagtctaaa aaattctttt tgattattat
     3721 ttttaattgc ctctacagaa ctcattggct cactcaagtc tctccaagcg tctgttcgtt
     3781 cccaatcgac ttgatatgag ccagacaaat tactttgttg atgtaaatta gctgatgact
     3841 taatcctctt gttttcgaaa tgttttttca atagattaac gatttcttca taaccttttt
     3901 tacgagcttg ctccaatggc gttgttttct cctcccatga tgggccaaca cccgcatccg
     3961 ctccagcttc tagcagagca gccactgttt ccacatgcct attttcaacg gcaacataaa
     4021 ctggagttat gccattgctg ttcggtatat ccacatctgc gcctgcatat ttaagttgag
     4081 tgattgctgc cgcatgccca ttttgagcag cgataaaaac tggcgttctg ccatctctat
     4141 ccggcgtatt cacatttgca cctgcgattc tcagttcgcg tatcacatct gctgcatacc
     4201 taaattgagc agcggcataa actggagtta tgccattgct gttcggtata tccacatctg
     4261 cgcctgcgta tttaagttga gtgattgctg ccgcatgccc attttgagca gcgataaaaa
     4321 ctggcgttct gccatactta tctagcttat tgacatcagc tcctgcttta accaaagggc
     4381 ctataaaatg accataacca ttagcagctg cccaatataa agggcttccc tcacattctg
     4441 aatcgacctc atcaataaat tttttcccaa ataggtctgt ttttttatct aaaagtctaa
     4501 aaacttcttt tttattattt tgtttaatgg cttctatcaa gttcatctac cgtcctccga
     4561 aatgtcagca ttggcagatt ataccaaaac ttgcattatt aataaaaaat ctattacaaa
     4621 cccagaaacc tgatcagttg tggaggttcg gtagccgatg ttcattttca atcacaaagc
     4681 tctagaggtt acgatagaaa tgctatcata ttaaaaaaac taaccctatt gataggattt
     4741 tgataggggt tgcaaccgct cattagggcg aactctatta tgttatgcag catgtggatg
     4801 atttttgatc cattctttta aagcttcatc catccgtatt tgccagcctt tgccagtggt
     4861 cttaaagtac ttcacaactt ctgggctgta acgcaaagta acaggtactt tcgttggctg
     4921 tttttgctgt cctcgctgcc ccacttttcg gcgaggtaat aatgttaata aagaatctgg
     4981 caacacctct ttagctgggc gcatcgctct cagatcttct cgggttaatt ctcgcacttc
     5041 tccaatttta ttggttaatg gtttctttct catatcgttt cacctcacgt aaattagctt
     5101 ttctaaaact gatcgctctt atccctcctt taataggcgt aaaacaaacc acgtgtaaac
     5161 gttgtcctat atacccaatc gtaataaacc gccgttctgc atataaactc ctaatatctt
     5221 cagcataaat cgctgtctcc caatcaaact cgactattag ctcgaatgcc aatcctcgtt
     5281 ctcggatatt tttattagat ttttcaggat caaaatcaat tttcatataa tttaattgta
     5341 gttacgaata aataaaaagt caatatgatt tataaattct tatttggcgg agtgtctttg
     5401 tcacaaatcg atgttattat cctttatgga acatagaata aaatacgaat taagtgactt
     5461 atctgagctt gtttttgagc cagattgctt tgtctcataa tcgaacgttt aggagactaa
     5521 taatgttaat gggatatata cgggtatcaa aacaggacgg gtcgcaacaa actgatttgc
     5581 agaaagatgc tcttctctca gtgggtgttg atgtagcgaa tatttatcag gactatgcat
     5641 ctggtaagat tgatgcacgc cctggtctct tatctgtgtt aaaagcttta cgtgctggcg
     5701 atacccttgt tgtatggaag ctagatcgtt taggccgaaa cttgcgccat cttatcaata
     5761 cagttcttga attagtagag cgtggtattg gttttaaagt attaacgggg catggggcat
     5821 ctattgatac aacgacaccc gctggaaaat tagtgtttgc tatttttgca gcacttgcag
     5881 aatttgagcg agaattaatt tcagaacgta cgaaagctgg acttgcttct gcacgtgctc
     5941 gcggtcatca aggtggagct ccattcaaga tgactgctgc taaagtaagg ctctctatgg
     6001 tagcaatggg aaaaccagaa acaaaagtag gtgatctgtg ccgtgaactt aaggtgagcc
     6061 ggcaaacatt gtatagacat gttgatccaa atggtgcctt gaggaaagat gggaaaaagc
     6121 ttttaaaaag caaaaaaact aatcttatct atctgactta actgttccgc tgggtctcaa
     6181 accccatcta caaaagctta acgtgcttta aattccgttt tctgaggcga ccccagatta
     6241 aaacactaaa attttactag ttatatattt aactaatttt aatccacata gtgatttcta
     6301 gtgtccgtat cttgttcctt ggtttaaagg ttgcctttcc tggttggaaa gggcttcagg
     6361 agtactcgga tcgctctggt taaactgggt taatataagg gggttatttt gttcaaaaga
     6421 attatgattg gccaaatgga caacaacagg agagttaacc ggaggctcat cagaattttg
     6481 cctagcttct acagtttcaa tactggaaac gttatttcct agagagaagg tttcatcaca
     6541 attccactca gcttgtaatt ctctagctaa ttgttcatct ttctgtattt gttctgctaa
     6601 agatttttgt aatttactaa cttcattctg tgcaagattt cttttagtaa cagctagaat
     6661 ttcgttataa ctcactctaa acttcatttt atccaatgta gccgaatcct ctgcttgcca
     6721 gttctcaaat aataattgca taagttcttt taacgctttg gaatcaactt gtgcagcttc
     6781 ttgcaaggct gtacgaattg tatttaaatg caaggcgtga tcatcattgt aagcagagtt
     6841 atgcttaaaa gcctctaata acccaatttg gattttctct ggcaaaataa ataagtgctg
     6901 ttttatagaa aatattctat taagctcttg cgtagaaaac ctaataggat ttgattcata
     6961 gagataacca agttgttcat gaagggtggc agtacttgga cgattgtttc gttgacgttc
     7021 attaaaagca ttatgaaaat ttgggttata ttgccttaaa gcctctaaat gtaaactacg
     7081 aatgatttct gccgcttcgt atgactggat acccagagct gctgtcagta aattttctat
     7141 agtatacgcg ccgcaactag tataatcgct ttgctgagac aatacgcggg ttttaaaata
     7201 tactttagga taaatctttt gtaattgtac ttggaatatt tctggaataa tggggcgagt
     7261 tgtaaacgag tcaatatatt cagcgcgcaa tacttttcca tctgcgctaa actctagcaa
     7321 cattcctacc caatgcgcat tccctaaatt acagggtatg agacaaatac gttcgccatg
     7381 gtgatttctt gcttcttgcc ttaatctgtt ctccaattga acactcataa tattatcaat
     7441 agcagccagt atttctatag gcttaataaa tagctgagag ttttgtattc gcaactgctg
     7501 caagcgagca gatacaatag cttgaatatc attgtcttca tattgatagg aaatattaat
     7561 agttgttttg ctggtttccg taggtttaga aagaatgttt actgaagagt ttatcatctt
     7621 cgatatctct gctgtactaa ttagactaat acatctgagt ccagtcctta attcggtgat
     7681 aagttcctca cgctgatcta tacgggtact agccagagcg ataatactct gatggtcacg
     7741 caattcggca ctctcctccc cccaccgcca acccagagta cctcccactt tcttccgaac
     7801 ccggcttaca ggctgagcac ttcggtaaac tccaaactgc tttcctaagt tttcgtcata
     7861 gaaccaatcc tcccctgttt caatcgtgcg agttcggggt agggctacgt cggcatcctt
     7921 gaaattgggt acggggtaaa atgggcgtat ggggtgacaa tattcattta ctacatgtgc
     7981 agggagtaat cgttgctccc cgcccacttc ttgtacccag gcagtattgc catcggtata
     8041 ttttttagca ttgtataaat gaatggtctt ctgataggct tgaatgagat tgtttaaatt
     8101 ggcgtgaacg ccatgttgtt ttacccaaaa gcctttttca aatccttgag cttgctcccg
     8161 ggcagcttca ttaggaaggt attgtttaat catcgtccac atgtgccaat ctaatgccca
     8221 taccgcatac tgaaagcctg tgatgccatt aaatatgcga ttggataagt cgatgacatt
     8281 tcctgatact aatgctaagt cgcggttgct ctttagcata gctttcgctt tgccttgttc
     8341 tccttctgca acaaatgtta gaaactctgc aatatcattt ggattcacat agggtacaga
     8401 taatgaggca tgaatacgca ccgagcatgg tgatgcttgt ggtgaaaatg ttaatgcagt
     8461 aggactgctt tggttaggct cttgaacagc aggttgtaat agttttatgt gagcttgcat
     8521 atcagcaata agcttttcat ttttaattcg ctcttgctcc attgtctgtt gtatataaaa
     8581 aatctgagtt ttaaaacctt gctcttggtt ttgaagagcg gtctttaaag ttgcgatttc
     8641 actcacttgt tctacaaaca cctgatgcag tctgtccgtt tcttttaatt tatctttttt
     8701 aagtcttctg actgtttgca caatgaaatt tggttttttt tgttctattg ataaatctat
     8761 aggcgttttg ccttgctcat ttttgatttt atgatatgcc cctgcttcta ggagtaagga
     8821 aataacatta tcctgtccga actgaaccgc tagatgaaga ggcgtatcgc ctttagcgtt
     8881 ttgcacgtca attaaagcta aagcgacttc tgtattttgt aggagatatt cgagcatctt
     8941 tacatcacca cgacttgctg caatatgcaa agcagtttgt tgctgattgt tttttgtttt
     9001 tatatcagct ttcattttta ataaaagctc ggtaagcctt atattacctt gtattaccga
     9061 taacattaat gacgtgttgc cttctatatt acgactttct aaagctgcct tgcgttctaa
     9121 caaccatttt atcgattctt ctaagttgtc tttgttgtct ttaataacca ttcggtgcaa
     9181 taaagtgttt tgggtatcag attccggctg ctcaacttcg gcaccgagac ttaacaataa
     9241 ctcgcactca acgtaatcgt ttctatttaa atttaaggct ttttctaata acacatttaa
     9301 gtgctttggg ttaagtcgaa ttccgtcaag tgtaaagata aaattatttt tttgctttaa
     9361 gatgtttaat agcatctcta cgagttcagg cgagctaaat tcacaagcga gatgtaaagc
     9421 tgtataattt ccttctaact ttaaagttaa taaacgccta tctttattta accactgatg
     9481 aaccacttga agttgattct gcttaatcac cattacgcat ttttctaccc aagctttagg
     9541 taggtaaacc tcatcaccat cataaagttc aggattattt tctaaaaaat ctgatatatc
     9601 gctacgctta tcatgattga cagtcagatt tttattctca agatttacac ctgtttttgg
     9661 gcttgtatta tgggtctgta gccattcatc aattttttct cgttcatagg tatgaccatc
     9721 tgatgtgaaa accggttcca tcataatttg acctgtaatg gaacaacggt atttttctgg
     9781 aattatgtta tctttaggca tgggtatatt ctccgtaaca atctgttaaa agttatcaat
     9841 aacacctgaa actcatcgtt attatctaaa ctaagtccaa ttagcttgaa tgattagctc
     9901 aattgtacat aaatctaaga tttttaacta taacactttt attggttttt tctatctata
     9961 ttattttaac aagttaatca acctattgtt ttttagttgg aatatccttc tttttcacag
    10021 attcttccca cgctacaatt tcacaagtat attcgctaaa aaatggatgt gctctatttt
    10081 tttgtgcgaa aagataaaat ttgattatcc acctattcct aaatagacct gaaacttttt
    10141 tatctatcat tagggcgagg cttattgata ggcgataata tgctatttaa ctggcatatt
    10201 cattccttgt tatgccattg ctcaaccttt cccaaaagct ttcctaaaag cttggctcag
    10261 ctggctagca ggtttttttg gaacaggcgg cgggtccttt tgttcaagga cactggatgg
    10321 cttagcagaa aaagaaacga ccgggttcat aacccttact ggctttattc cattagggta
    10381 ttgtttaaaa tgcgcttcca atatcttaac cgcctctctg tgctctgggt ctttgccttg
    10441 cctggcgaac gataaggctg ttccccactt cgttttaatg ctcgcgttcg cacctgcttc
    10501 cagcaatgcg gtaatagcta atgtatgccc attacaaaca gcgatataaa ccgaggttat
    10561 gccgtccggc attggtgtat tcatatttgc tcctgcggca tgcagggcag ctatggctga
    10621 cacgtgacca ttttgagcag cgataaaaac tggcgttgca ccatcgcatg gcgtatccac
    10681 attcgcacct gcggcataca gggcagttat ggctgacgca tgcccattgt aagcagcgat
    10741 aaaaactgga gttttgccat ccgatattgg tgtattcaca tttgcccctg cggcatgcag
    10801 ggcagctatg gctgacacat gcccattgta agcagcgata taaaccggcg taaagccacc
    10861 atcttttgac gtattcacgt tcgcgcctgc tatatacagg gcagttattg ctgacgcatg
    10921 tccattgtaa gcagcgacat acgccggcgt ggcaccatct ttttgcggca tgttcacatt
    10981 tgcccctgcc gcgtgcaggg cggttatggc tgactcatgc tctttttcag cagcgatata
    11041 aactggtgtt gcgccatttc tgtttggcgt atccacattc gcccctgctg tatgcagggc
    11101 agtgatggct gacgcatgtc catttaaagc agctttataa accaacgttc ttccctgttt
    11161 atcttgctta ttaatatctg cacctgcttg aagcaaaggg tctataaaat ggatatggcc
    11221 aaaagcagct gcccaataca aagggcttcc gttatattct gaatcgactt catcaataaa
    11281 cctttttcca aagcggtctg tctttagttt taaaagtcta aaaaattctt cttgattatt
    11341 ttttttaata gcttctataa gacttgtgat tgattttaaa tcgctaatac tcactgagga
    11401 agactcaaaa tcctgaaact gagttaagat aggcgtattc ttttgttctg aagggttaag
    11461 ttttaaagcc gcttgaggcg tattccttgg aacaggatca ttaaccgtag actgaggtgt
    11521 tgctgaaact ataggttttt gaggagtgtt ttctataact aaagacttta acatctttga
    11581 tatctcttta gcagcagctt cggcatctaa gggttcatcg tttatataaa gtatatgttt
    11641 attttgggtt gttttcagcg ctagaatata aagcaccgta tattgtattt ttaggaaaaa
    11701 ttgttaaaat tgcagatgaa actactgaaa atataggtag agagggtgtc ttagaacgta
    11761 tctattggag ggagggggaa ccatgggttc gaatccgcag ccaagacggg gtttgttcgt
    11821 ccgtaaaatg gaacgagaca aaccttccgc ccatcataga cttgatgttc aaaaaagaac
    11881 tccctttgct atctctacca gccttgaaag atctagcgaa ttatctgaag acctatgcca
    11941 agcttttgcc aaaacagcgg cactccaaga agagatgatt tgtttaatgg ctgttttatc
    12001 attgcggatc attcactcta aaggagcgaa aaaatgactc attctctttc taaaattagt
    12061 gaccaccatc gccaacgaag agcctgtgta tacattcggc aatcaactac ttttcaggta
    12121 atccataatc gagaaagcac agagcgccag tataatttac gccagcgagc tatagatctc
    12181 ggctggaatg aaaatttaat tgatatcatt gataacgatc agggacagtc tgcgaagtct
    12241 attgagcatc gagaaggttt tcaaaaacta gcttctgaaa tcgttgctaa acaagtaggc
    12301 atagtactga tgttggaagt ttctagacta tcacgttcgt gtagtgattg gcatcgcttg
    12361 atagaaattt gcagcattac gagaaccttg attgcagatg aagcaggaat atatgatccc
    12421 agagagccaa atgacagatt gttgttaggc gttaaaggaa ctttatctga agcagagctt
    12481 tttacattaa gaactcggct ttatgaaggc agatggaata aagctaaaaa aggtcagtta
    12541 ggccgttcta ttcctacagg ttatataaag gatgaaaaag gtaattggac caaagatcct
    12601 aaccttcaag ttcaagagag gatgaattat atatttaaat tgtttgccca attaggtata
    12661 gcaaggcgag tgctattggt acttaaaaat gagaacttaa aaattcctac aaaggtctgg
    12721 ggaggtcctc aacacgggca actaatttgg aaagaggcta gtttaggaag tataatgagg
    12781 ttattaaaaa atccctcata tgcgggagtt tatgtttatg gagcatgtga ctatgaaagc
    12841 aacggatatt atgctaaaac agggaaagca aagccaaaag ctcgtccttt aaaagaatgg
    12901 acagtttgct tacaagatca ccatgaacaa tatatttctt ttgatgaata tatgagtaac
    12961 caggatcgtt tgcatcaaaa ttggtttcgt tctaaaacaa gaggagcacc tcgagaaggg
    13021 agagctttgt tacaaggcat agtttggtgc ggtcaatgtg gctcgaaaat gggaattaac
    13081 agctattctg ctaaagagag acgaaatagt tatacatgta caaaagatta tcaaaacggt
    13141 gctcaacatg tttgccaaac tataagtgct aggacaatcg atgggttaat tgtttctcta
    13201 tttttagaag ctatggcacc ttcacaactt gaaattgcta aaaaatcgat agaaaaaaca
    13261 caaattgaaa aacaggcgtt aaaaaaacaa tgggaacagc aactaacgca agcgcgatat
    13321 gatacacaat tagcgcaacg ccaatatgac gcgattgatc cagataatcg tttagtggct
    13381 tctgaattag agcgtcgttg gaatgaaaaa ttagaaatac ttcaaaagtt agaagaaaat
    13441 tatgaggagg ctaataagca atctcgtttt tctttaacgt tagaagaaat aaaagaaatg
    13501 gaaatgctta taaaagacct ggataagatt tggtatgctg ccactacgac cgatcgtgaa
    13561 cgtaaacaat taattcgttg cgctatagca gaagttcaat tgaatggaat tactattcct
    13621 caaaaagttg aaattcgcat tacctggcgc tctggagcag tgagtgaaca tattgttgat
    13681 cggataaaac aagggatatg ggcacctaga acatctgaca tcgttattga acgaattaga
    13741 atactttctg aaaaatatac ggtaaataag attgcgaaat tgttaaacca agaagggcat
    13801 cgtagtgctc ataatagact ttttaaagat taccatattc tctatatagc acgacggaat
    13861 agcatacacg ttacgactca tctaaaaggt ataaaaaatc caaattatag gaggaaaaaa
    13921 ttaaaggact aatgggctta ataggcattg actaaagcag aatttttatt taatataatt
    13981 gggggtgggg tatgtattgg gactgttata agccagcaga gcattattat tgtctttctt
    14041 taagtattca ttataaatag catagttttc ggttttttcg cctatgtatc gctcttctat
    14101 aaaaaatgtt cttagctctt ctaatacatg ttgtgttttt tcttgttctt ctttgcctgt
    14161 gagattcagt ttcccgcttc ctcttctacc ggctggcacg gctgcagggc tagcctgaag
    14221 atttaaatca aggatgtggg cggaagtaga gatcgatatt gcagcagttg ggcgatggga
    14281 agcttcctct aatgttttag catctgtagt catgaacttt gcaatgtccg aagccgatgg
    14341 gcgagattct ggattgcctg cccagcactg ttggattaaa tgtgctaatt tttctgggca
    14401 gtctgaaggg atagcttctc gttttccacg tccaacaaac acagggatgg cttctggggt
    14461 atcttcagcg tagggaatct tgcgtgaggc tagttcccaa aaagtgatgc ccagactata
    14521 aatatcggat ttataagtat actgttctct ctcaaataat tcaggagcaa tccaggggag
    14581 tgtgccaacc agttctttgc ttttttgatg attctttgtt aaagatttgg tttcattttt
    14641 gactttcgat aatccaaaat cggttagctt cgctttttga tcatctaaca atatatttaa
    14701 gctttttata tcacggtgta aaatagattt actgtgtaaa aatgctaagc cagaaaccat
    14761 gtctatggca atgcgaatgc gaacatccca gtctaaggct tgtgttttgt cttgcaaaac
    14821 actaaacaaa gatcctttag gcatgtattc catgaccaga catctaggga tgctgcaata
    14881 cccataaaaa tgcacaatat taggatgacg caattgtgcc atcatatccg cttctttacg
    14941 aaactcctca tgtgcttctg gacttaggtt gcctatttgc aatttcttaa tcgcaacact
    15001 tccaaattgg tagctacctt gatacacaat tccaaagctg ccttgaccca gttctttgcc
    15061 aaaatttaag tctttgtact ctatttgaca agaaacaaga aaaccctttt cagtattttc
    15121 ggctggtttt tgattaaagg catctagaat cggttgccaa tcagggctgt cattattttc
    15181 agaaagtatt tggacggtag gaaacatatg aatactcttt tgctgagtta acttaagcgt
    15241 agcctgtatg atttcagcac agaagtcaag atgccacaat taggactggc aagctcagag
    15301 ccttaagacc tctaccctgt gcttaaggta cattataatc ccttagagta acttttcatt
    15361 ccaatgcttg catacctcga agaccatagt ctttgcaacc gcccgcaagg gggaaaacaa
    15421 gccgttgctc atctcagaaa ataccatgtg catccagcgc gttagtcaaa tgctttttat
    15481 tgaacaccaa aaagacaaca taagtttcct tatttcaagc gaattttctc tttaagtgca
    15541 tcaatcgaaa ccgtggtatg agatatccaa ccagtccctg cagcaaatcc aattaaaaag
    15601 atccctagaa caataatagc ccataatctt aaggaataat ttctttcgaa ctcctgtatt
    15661 tctggaataa cactttttgc ataagataat gaacgattta aatcttgcat aagatagtct
    15721 aactctgcat ggcaatattt tttaacttct gttgcagtcg tattgacagt gacactgaga
    15781 gcgattttgg cttctttttg aatactttct ttaataacct ctttgacttc atcaagactc
    15841 tcgatttttt catcaaacgc ttcaatggtt ttgagcagtt cagcttctcg ttgacccacc
    15901 atttttaatt ctaaagacca attgtcgtaa agcttaaaaa attgataaag tacatcttcg
    15961 agatttgcag
//
LOCUS       DAORVT010000002        12112 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_2542_length_12112_cov_6.002986, whole genome shotgun sequence.
ACCESSION   DAORVT010000002 DAORVT010000000
VERSION     DAORVT010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 12112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12112
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2542_length_12112_cov_6.002986"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..516)
                     /locus_tag="PK389_00060"
     CDS             complement(196..516)
                     /locus_tag="PK389_00060"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HQY22138.1"
                     /translation="MNRGDIYWVDLEPTRGSEINKLRPCVIVGATPINKARHTVVVVP
                     LSTSAKTRPPLVIPVHCLDQIASVVCDQIRAIDKSRLVKYAGIISTKDLQALEEGLRQ
                     VLSL"
     gene            complement(503..739)
                     /locus_tag="PK389_00065"
     CDS             complement(503..739)
                     /locus_tag="PK389_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22139.1"
                     /translation="MQKMLISVPEPLAIRFRSIIPTRQRSQVISHLIEKEIEKREKKL
                     YECALEVEGDKSLNREMKQWETKTLQDGLKHEPW"
     gene            complement(1060..3264)
                     /locus_tag="PK389_00070"
     CDS             complement(1060..3264)
                     /locus_tag="PK389_00070"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HQY22140.1"
                     /translation="MPSSAKLRNDTLLCTVYEGDIEKVRLLIAQGLNVNAISYSGMTA
                     MHIAAQEGQVEAIKVLQAAGANINSFDKDGGTPIWIAAQEGQVEIIKVLKAFGADVDK
                     QLNDGRTPLYVAAQNGHVECINALYAAGANVDKPNKNGETPMHTAAKNGHTEAITALS
                     VVGANVNTPNKNGETPLYVAVLNGQLEAIEILRTLGANLNMRTREGATLIHAAALNGH
                     AETIIALHALGVNVNTAMRDGSTPIYIAAKKGHSKVVTALYGLGVNVNAFNQKGRTLI
                     HLAVNNGHASVLFALQAAGADVNASSPLDGTTPIHVAAIKGNAEMIRVLHTLGAHMNV
                     TNNNSTTPIYMAAQKGHASAINALHTAGADVNVVHRNGTTPIFIAASYGHVEAIHALY
                     AAGANLNVRTYFSGLTPVYVAAQEGHASAIGALCALGAYADIPNEDNDATPIYIAAEK
                     GHAAVITALHAAGANANMQQKNGTTPLSIAAFHGHADAVCALHAAGANVNTPTRDGFT
                     PIYIASLKGRLSVVTALCAAGANPNTLSRDNVMPIYIAAQKGFASIITALHTAGANVN
                     ILHRETTPIFVAAFFGHEKAIYALHAAGADVNAPTSNGSTPIYIAARKGHASVITALH
                     AVGVNIDTVHKNGITPLYLAAFYGHAEAIRALRAAGANVDTPNRNGLTPLHIAMKKGH
                     APAIAALQTSGQNTAIKTQSDILLFSNKPSIYLNQPSVYKVARPLGKLSKKH"
     gene            complement(3318..6467)
                     /locus_tag="PK389_00075"
     CDS             complement(3318..6467)
                     /locus_tag="PK389_00075"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HQY22141.1"
                     /translation="MPKDNQIPERYRCSITGEIMIDPVFAADGHTYEREGIAQWLKIN
                     DTSPKTNIRLKHKELTDSHYTRSDVLEFLDSYPELYEGDEIYMPKSWMSGFARAIKEN
                     QLQDVQRWLDKDRRLLTLKLEDNATALHLACEFSSPELVEVLLNILQQKNKLVMGGEI
                     KFKSTHLNVLLQQALIRGDCPKCELLVGLGAEVEQPEAITQNTLLHQMVIRDNLEAIS
                     WLLERNAILESRNCDGNTPLLLSVIHKHKKISEFLLKMDANLEVKNLQKKLAFYIAVE
                     KNDLEMAKLLLAYGANPALIYGENQLSVLHLRAQDKADVMLEYLLETKAIVLIDIQDA
                     NGNTPLHLAVKSLNHKAVLVLLEAGAYHKIKNEENQTPIDLAIASQSLEIADCIFQKV
                     RNFKKSKLKETDRLHRVITAQNIKIRNLEAVLNGALALEEKEENETIQKIKLDRDPLK
                     IKENNSKSDISFIEAIKQGNQEEIFRVLTIKTDSFGEKFIDEVDSEYGGSVLYWAANY
                     GLIHLIDSLVKEGVDINQQNKNGETPMYAAARNGDPQVITLLKSLGAAVDIPDEDGAT
                     PVYIAAYEGNVEAIIALNAAAADMNKPDNCNHAPVYAAALNGHAKSIAALRASGANID
                     ALNNKGETPLYIAAEQGHKEAIVALISAGANVNTLNHDGETPIFSSAKNGHSSAITAL
                     HAAGADVNTKNKHGLTPICFSAENGHAEAVTIFHTLGADVNTPCTSGLTPIFFAAENG
                     HASTIAALRAEDANVNARNNDDETPVYVAAKKGHAAAIAALHAAGADVNATKSGITPV
                     YVAAENGHTEAITSLITAGADVNILKPDGTTSVYAASLRGYAETIVVLHAAGANVDTG
                     NNNDETPVYAAAKHGHATAIAALYTVGANVNTANHDGMTPVFVAAQNNHPSTITALHA
                     AGANMNTAIPDSRTPMFMAAQLGNPEAITALLAAGADANIKAQWGTALEYAKRGKEPG
                     QLEVVRRLEAHLKQCPNGVKPIEAHKNDVVFRANVTSTAEQKNPPVLIQFEQKSAILP
                     QKYFPKHSIWNSKNRRNRN"
     gene            complement(6662..9088)
                     /locus_tag="PK389_00080"
     CDS             complement(6662..9088)
                     /locus_tag="PK389_00080"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HQY22142.1"
                     /translation="MPQDNKIPEKYCCSITHQVMIDPVIAADGYNYEREAITQWLQTH
                     DTSPKTNEKLDHKNLTTNHDKRSDILDFLDSYPELYIGDEVYLCKSWIAEFITAIKQN
                     KLQEVQRWLDKDKRLLTCKLEDDFTALHLACEFSYEVVDILLKTLKQRNQSILPGEIG
                     FKPVHLNLLLERALSSRDHFHYDLLLSLGAELEQPDALQNTLLHRMVVNNNPEAVSWL
                     LEQKTVLESYNSEGNTPLLLSVIHNNKELVEFLLKKGADPKVKNIKQQIPVLIALLNR
                     NESILCLLTGTDKATLPSLHLALELNDNAVIEALLKQKIGAIEAQDERGRTPFYSAVE
                     RGNLEAARLLLSQGANHNVSNVSCGIKKLNVLHVATERADVEMLKYLLQTEVADLIDV
                     QNSKGDTPLHIAVQFGHNDIITILLEAGAYHKVKNEKDQTPVELARFQKKLKIANLIV
                     QIVRELKKDRLKETKRLRQVVLEQTNEILSLKASLSRLLNGKTPLYIAAQDGDAKAIA
                     ALHASGVNLNKPDHEGLAPIYIAAQNGHMSAVIALIDAGVNINALNENGRTLICFAAQ
                     NGDASAIATLAAAGANVNTPDCERTPICWAAGNGHTSAISALIAAGVDVNTPNSNGST
                     PICFAAQNGHMSAIVTLIAAGANVNTPGSGNRVPICWAAENGHTAAISRLIAAGANVN
                     SLDQNGVTSIYVAADNGHAAAVVALLDAGANANLMNNSYETALGVSKKRENREVVEIL
                     EAHFKQYPSGIKPVQVIKNEEVCFHEKSSNLLEQKNPPILIQFEQKSAIPVQKDLPKH
                     SKKVSKNRKQ"
     gene            10052..10303
                     /locus_tag="PK389_00085"
     CDS             10052..10303
                     /locus_tag="PK389_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005373128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HQY22143.1"
                     /translation="MSNANKILKKMRLNPKDWRIEDFETVAKQHGVTVRKGGGSHAIF
                     DHPMWVELLSVPARRPIKPIYVKKFIALIQILEKNYEQD"
     gene            10290..10655
                     /locus_tag="PK389_00090"
     CDS             10290..10655
                     /locus_tag="PK389_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005992222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HQY22144.1"
                     /translation="MNKIEYPFTVCTLPQSEGGGYLIEFPDLPGCMSDGDTLEEAITN
                     GSDAMACWISAAKEIGREIPNPVEPRTGKWVQRVPRSLHSRLVARAKNEGVSLNTLVV
                     SMISESLGKRDPDHQHQRH"
     gene            11096..11386
                     /locus_tag="PK389_00095"
     CDS             11096..11386
                     /locus_tag="PK389_00095"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HQY22145.1"
                     /translation="MGTKLKSYDKEFKERAIKLALASSQPISKTAKELGMNEATLYSW
                     VKSNRNAYVEQHGNNGKEILDELAKLRKENARLREEREILKKAAVFFVKEAQ"
     gene            11479..>12112
                     /locus_tag="PK389_00100"
     CDS             11479..>12112
                     /locus_tag="PK389_00100"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HQY22146.1"
                     /translation="MYRGISSREQANNKLTKQIRSVHQESRKIYGGRRIYAELRAQGI
                     SCSKNRVVRLKRKAGIMAKTRRRFKVTTDSKHELPISGNILNREFNVLAPNTHWVVDI
                     SYISTLEGWLYLAVVVDLFSRKVIGWEMEDNMKTGLVKSALLKALKGRKISKGLVHHS
                     DRGSQYASFEYQSLLSAHQITSSMSRKGNCWDNGAPRMTSQRVVNATYKMK"
BASE COUNT         3452 a         2482 c         2275 g         3903 t
ORIGIN      
        1 aagtcgatag aactgggcat cctctattca gatggcatga atttagaaga cgaagcaata
       61 ggttgaagaa tggatgttta gatgttttga agaagccaaa gaaatgttac cagctatcgg
      121 ctctattcgc atagggattt gtagatgatc ttcccctaaa attttcagga taataaaaaa
      181 tatcatggct agatctcaga gtgagagaac ttggcgcaaa ccctcttcta atgcttgcaa
      241 gtctttagta gatattattc cagcatattt gactaatcga ctcttatcta tggcacgaat
      301 ttgatcgcac actacagaag caatttgatc taaacaatgt acgggaataa ctaagggtgg
      361 cctagtttta gctgaagtag ataatggaac taccacaact gtgtgtcttg ccttattgat
      421 tggagtggca ccgacgatga cgcaaggtct cagtttattg atttctgaac cacgagttgg
      481 ctcgagatct acccagtaga tatcaccacg gttcatgttt caggccatct tgtaacgttt
      541 tagtctccca ttgcttcatt tctcgattta aagatttatc accctctact tctaaggcac
      601 actcataaag ctttttttcc cttttttcga tttccttctc aattaagtga gaaataactt
      661 ggctgcgctg acgggttgga ataatagagc gaaaacggat agccaatggt tctggaactg
      721 atattagcat tttttgcata gtgtatatca cctctcaact tatatttaaa gatatattat
      781 atcttatatc taatctcata aaaaagcaag agtgcatatt ctactcgtgc ttgatcagca
      841 acccctccaa aaatggccag catctattcg cataaaatag catcagggtt ggcccggata
      901 agttaagcac cttaagttct ctaatttagg cttaacgtgt tttaaattcc gtttcctgac
      961 gccacccctt ttaggattcg atatattgta acttctccca ttttaagctg attggcaata
     1021 ttgtctttag atggttgttt ttttttattg ctccatctct taatgcttct tagagagctt
     1081 gcctaaagga cgcgcaactt tgtaaacaga gggttgattc aaataaatag aaggcttatt
     1141 agaaaataac agtatgtcgc tttgagtttt gatcgccgta ttttgacctg atgtttgcag
     1201 ggcagcgatg gcgggagcgt gccctttttt catagcaata tggagcggcg ttaagccatt
     1261 tctgtttggt gtatctacat ttgcacctgc tgcacgcaaa gcgcgtattg cctctgcatg
     1321 accgtaaaac gcagcgagat aaagaggcgt tataccattc ttatgtaccg tatctatatt
     1381 aacacctact gcatgtaggg cagtgatgac tgaagcatgt ccttttcgtg cagcaatata
     1441 aatcggtgta gaaccgttag acgttggcgc atttacatcc gctcccgctg catgtagggc
     1501 atatattgct ttctcatgac caaaaaaagc tgcaacaaaa ataggcgttg tttctctgtg
     1561 taaaatatta acattagccc ctgccgtatg tagagcagtg ataattgatg caaatccttt
     1621 ctgagcagca atataaattg gcattacatt atctctgctt agggtatttg ggttcgcgcc
     1681 cgctgcgcat aaagcagtga caaccgaaag acgtcctttc agtgaagcga tataaattgg
     1741 tgtaaagcca tcacgtgttg gggtattcac attagctcct gccgcatgca aagcgcatac
     1801 tgcatctgca tgaccatgaa aagcagcaat actaagaggt gtcgtaccat ttttctgttg
     1861 catatttgca ttagcgcctg ccgcatgtaa agcagtaatg actgcggcat gtcctttttc
     1921 tgcagcaata taaattggcg ttgcatcatt atcttcgttg ggtatatcgg catacgcacc
     1981 taatgcacaa agagcgccta tagcggaagc atgtccttct tgagcggcaa cataaaccgg
     2041 cgttaaacca gaaaaatatg ttctcacatt caaatttgcg cctgctgcat ataaggcatg
     2101 tatcgcttct acgtgaccat aagacgccgc aataaatatg ggcgttgtgc catttcggtg
     2161 taccacattc acatctgcgc ctgctgtatg taacgcatta atggctgaag catgaccttt
     2221 ttgtgcagcc atataaattg gtgttgtcga attgttattt gtaacattca tatgagcacc
     2281 caaagtgtgt aaaacacgta tcatttcggc attacctttg atggcagcaa cgtggattgg
     2341 cgttgtgcca tcaagaggcg aagatgcatt cacatctgcg ccagcggcct gcagcgcaaa
     2401 aaggaccgaa gcatgcccat tattaacagc taaatgaatt aatgttctgc ccttttgatt
     2461 aaaagcattc acattcacgc ctaaaccata tagagcagtg acaacttttg agtgcccttt
     2521 ttttgcagca atgtaaattg gtgttgaacc atctcgcatt gccgtattca catttacacc
     2581 tagagcatgt agtgcgatga tagtttcagc atgtccattt agcgcagcgg catgaattag
     2641 tgttgcgccc tctcgcgttc tcatgttcaa atttgcacct aaagtacgta atatctctat
     2701 tgcctctaat tggccattca atacggccac gtaaagcgga gtttccccat tcttgtttgg
     2761 tgtgttcaca ttagcgccca ctacacttag agcggtaata gcttcagtat gaccgttttt
     2821 ggcggcagta tgcatcggtg tttcgccatt tttgtttggc ttatccacat ttgcacctgc
     2881 ggcatataaa gcattgatac attctacatg cccattttga gcagcgacat aaagtggcgt
     2941 tctgccatca ttaagctgtt tatccacatc cgcaccaaat gcctttaaga cttttattat
     3001 ttctacttgc ccttcctgcg cggcaatcca aattggcgtt ccaccgtctt tatcaaaaga
     3061 gtttatattt gcacctgccg cttgaagcac ttttattgcc tctacctgcc cttcctgagc
     3121 agcaatatgc atagctgtca tcccactata tgagattgca ttaacgttta aaccttgtgc
     3181 aatcaatagt cttacttttt ctatatcgcc ttcataaaca gtgcataaaa gagtatcatt
     3241 tcttaacttg gctgagcttg gcatttttaa accccttaaa atcaaaaata tcgattacac
     3301 ttaagaataa tatttactta atttctgttt cgtctgtttt ttgaattcca tatcgaatgc
     3361 ttaggaaaat atttttgcgg aagtatggcg gacttctgct caaattgaat taatacggga
     3421 ggattctttt gttcagcagt gctagttaca tttgcacgaa aaacaacatc atttttatgg
     3481 gcttctattg gttttacccc attaggacat tgttttaaat gtgcttctaa gcgcctaaca
     3541 acttctagct gccctggttc tttacctctc ttagcgtatt ctaatgctgt accccactgt
     3601 gctttaatgt tcgcatctgc tcctgctgct agcagagcgg ttattgcttc cgggttcccc
     3661 aattgagcgg ccataaacat cggcgttctg ctatctggga tcgctgtatt catatttgcc
     3721 cctgccgcgt gcagagcagt tatggtcgat ggatggttat tttgagctgc cacaaaaact
     3781 ggcgtcatgc catcatgatt cgctgtattc acatttgccc ctacggtgta cagagcagct
     3841 atagctgttg catgtccatg tttagcagcg gcgtaaacgg gtgtttcatc attattatta
     3901 cccgtatcta cgtttgcacc tgcggcatgc aggacgacta tagtttctgc atatccgcgt
     3961 agagaagctg cataaacgga tgttgtacca tccggcttta gtatattgac gtctgcaccg
     4021 gctgtaatca gtgaagttat agcttctgtg tgcccatttt cggcagcgac ataaacaggt
     4081 gttataccac tctttgttgc attaacatct gcacccgctg catgcagggc agctatagct
     4141 gctgcgtgtc cttttttagc agcaacataa accggggttt catcatcatt attgcgcgcg
     4201 tttacgtttg cgtcttctgc acgcaaggct gctatagttg atgcatgccc attttcagca
     4261 gcgaaaaaaa tcggagttaa gccactagtg caaggcgtat ttacatcagc tcctaaagta
     4321 tgaaatatag ttactgcctc tgcgtgtcca ttttcagccg aaaaacaaat aggcgttaag
     4381 ccgtgtttat tttttgtatt tacatctgca cccgccgcat gcagtgcagt tattgcgctt
     4441 gaatgcccat ttttagcaga actaaaaatc ggtgtttcac catcatgatt caacgtattt
     4501 acatttgccc ctgcagaaat aagagcaact atggcttctt tatgcccttg ctcagcagca
     4561 atgtaaagag gggtttcacc cttattattc agtgcatcta tgtttgcacc cgaggccctg
     4621 agcgccgcta ttgactttgc gtgcccgttg agagctgccg cgtaaacggg cgcatggttg
     4681 cagttatctg gcttgttcat atctgctgct gcagcattta gggcaattat ggcttcaacg
     4741 ttcccctcat aagcagcaat ataaaccggt gttgcaccat cttcatctgg tatatccaca
     4801 gctgcgccta aagactttaa taaggttatc acttgtgggt ctccattgcg agctgccgca
     4861 tacataggcg tttcaccgtt tttattttgt tgattgatat ccacgccttc tttaaccaaa
     4921 gaatctatta aatgaattaa accataatta gcggcccaat ataaaacact tcctccatat
     4981 tctgagtcga cctcatcaat gaatttttct ccaaaagaat ctgtttttat agttaaaact
     5041 ctaaaaattt cttcttgatt gccttgttta atcgcttcta taaaacttat atctgattta
     5101 gaattatttt ctttaatttt aagaggatct cgatctaatt taattttctg aattgtttcg
     5161 ttttcttctt tttcctctaa tgcgagcgcc ccattgagaa cggcttctaa attcctaatt
     5221 ttaatattct gcgctgtgat tactctatgc aatctatctg tttcttttaa tttagatttc
     5281 ttgaaatttc gtactttttg aaaaatacaa tcggcaattt ctaatgattg actcgctatt
     5341 gctaaatcta tgggagtttg gttctcttca tttttaattt tatgatacgc tcctgcttct
     5401 aacaatacta aaacagcttt gtgattcaag cttttaaccg ccaaatggag cggggtatta
     5461 ccattagcat cttgtatatc aattaaaact atcgctttgg tttctaataa atattcgagc
     5521 atcacgtcag ccttatcctg tgctcgaaga tgtaaaacac tcaattgatt ttccccgtaa
     5581 ataagggcgg gattggcgcc atatgccaaa agtaactttg ccatctctaa atcatttttt
     5641 tcaactgcaa tataaaaagc taatttcttt tgtaaatttt taacttcaag gttagcatcc
     5701 atttttaata aaaattcgct aatcttttta tgtttatgta ttactgacaa taataatggc
     5761 gtatttccat cacaattgcg actctctaaa atcgcgttgc gttccaacaa ccaacttatt
     5821 gcttctaaat tgtctcgaat aaccatttgg tgcagcagtg tattttgagt aatagcctca
     5881 ggttgctcaa cttccgcccc aagcccaacc aataactcgc acttagggca atcacccctg
     5941 atcaaagctt gttgtaatag cacatttaag tgagtagact taaacttaat ttccccaccc
     6001 ataacgagtt tatttttttg ttgtaatatg tttaataaca cctctacaag ttccggagaa
     6061 ctaaactcgc aagctaggtg tagagcggta gcattatctt ccagttttaa agttaataaa
     6121 cgcctatctt tatctaacca tctttgtacg tcttgaagtt gattttcctt aattgcccta
     6181 gcaaaccctg acatccagga tttaggcata taaatttcat ctccctcata aagttcagga
     6241 tagctatcta aaaattctaa tacatcactt ctcgtataat gactatctgt caactcttta
     6301 tgcttaagcc ttatatttgt cttaggactt gtatcgttta tttttaacca ctgtgcaatt
     6361 ccttctcgct cataggtatg gccatctgct gcgaataccg gatcaatcat aatctcacct
     6421 gtaatagaac aacggtatct ttctggaatt tgattatctt taggcatgag tacattctcc
     6481 gtaaaaatat aaaaattagt ttaactaaag caatttcaga agtaaatgct cttatatggc
     6541 attagccata taaataaggc tacgaatttt aaagtggact gtatttacaa aacagaattt
     6601 tttcagaact cgggctctaa ctcataattt tggatgatat taaaatccta ttttaaccgt
     6661 gttattgctt tctatttttt gaaacttttt tcgaatgctt aggaagatct ttttgaacag
     6721 gtatggcaga tttctgctca aattgaatta atataggagg gttcttttgc tctaataaat
     6781 tggatgattt ttcgtgaaaa caaacttctt cgttttttat aacttgcaca ggtttaattc
     6841 cagaaggata ttgtttaaaa tgtgcctcca atatttcaac aacttctcga ttctctcttt
     6901 ttttagatac ccctaatgct gtttcgtacg aattgttcat aagatttgca ttcgctccag
     6961 catctaataa agcaacaact gctgctgcgt gtccattgtc cgcagcaaca taaatagaag
     7021 ttacgccatt ttgatcaaga gagtttacat ttgcgcctgc agcaattagt ctagaaatgg
     7081 ctgccgtatg cccattttca gcagcccaac aaatcggtac cctattgcca ctacctggcg
     7141 tattcacatt tgcacccgca gcaattaggg taacaatagc tgacatatgt ccattttgag
     7201 cagcaaaaca aatcggcgtt gaaccattac tattcggcgt atttacatcc acacctgcag
     7261 caatcaaagc agaaatggct gatgtatgcc catttccagc agcccaacaa atcggcgttc
     7321 tttcacaatc tggcgtattt acattcgcgc ctgcagcagc caaggtagca atagctgagg
     7381 catccccgtt ttgagcagcg aaacaaatca gtgttctacc attctcattt agcgcattta
     7441 tattcacacc tgcatcaatt agagcaataa cagctgacat atgtccattt tgagcagcaa
     7501 tataaatcgg tgccaaaccc tcatgatccg gtttatttag attcacacct gaagcatgaa
     7561 gcgcggctat tgcttttgcg tctccatctt gggcagcaat ataaagcggt gtttttccgt
     7621 tgagaagtct tgaaaggcta gcttttaaac tcaaaatttc atttgtttgc tctaaaacta
     7681 cttgacgtaa tctttttgtt tcttttagtc tatctttttt gagttctctt acgatttgca
     7741 caataaggtt tgctattttt agttttttct gaaatcttgc taattctaca ggtgtttggt
     7801 ctttttcatt tttgacttta tgataggcgc ccgcttctaa aagaatagta ataatatcat
     7861 tatgtccaaa ttgtaccgct atatgaagag gtgtgtcgcc ctttgaattt tgaacgtcaa
     7921 ttagatctgc tacttcagtt tgtaataagt atttaagcat ctctacatcg gcacgctctg
     7981 tggcaacatg taagacattt aattttttta taccacaact gacattactg acattatggt
     8041 tagcgccttg ggataacaat aaccttgctg cttctaaatt acccctttca actgcactat
     8101 aaaaaggcgt tcttcctcgc tcatcttgag cttctatagc tcctatcttc tgttttaata
     8161 aagcttcgat aactgcatta tcgttaagct ctaaggctaa atgaagagac ggcagagttg
     8221 ctttatcagt tcctgtcaat aaacagagta tagattcatt tcgatttagc aaagctatta
     8281 aaacaggaat ttgttgtttt atatttttaa cctttggatc agcgcccttc tttaataaaa
     8341 attcgacaag ctctttatta ttatgtatta ccgatagcaa taatggagta ttgccttcac
     8401 tgttatagct ttctaaaacc gtcttttgtt ccaacaacca gcttactgcc tctgggttat
     8461 tattaacaac cattcgatgc aataaagtat tttgaagagc atcgggttgc tcaagttcag
     8521 ctccgagact taacaataag tcgtaatgaa aatggtcacg actacttagt gctctttcta
     8581 gcaataaatt taagtgtaca ggcttaaacc caatttcacc cggcagtatg gattgatttc
     8641 tttgttttaa tgtttttaat agtatatcta cgacttcata gctaaattca caagccagat
     8701 gtagagctgt aaaatcatct tcgagcttac aagttaataa acgtttatct ttatctagcc
     8761 accgctgaac ttcttgaagt ttattttgct taattgccgt aataaattct gctatccaag
     8821 acttacagag ataaacttca tcaccgatgt aaagctcagg gtagctatct aaaaaatcta
     8881 atatatcgct acgcttatca tgattagtcg tcaaattttt atgatcaagt ttttcgtttg
     8941 tcttaggact tgtgtcatgg gtttgtaacc attgtgtaat agcttctcgt tcataattgt
     9001 agccatctgc tgctataact ggatcaatca taacttggtg tgtaatggaa cagcaatatt
     9061 tttctggaat tttattatct tgaggcatga gcatattctc cctaataacc ttaaaaatta
     9121 ttcacaaaat ctagttgaac cgcttattag cacctaaagc caagtacaac tgatctgaat
     9181 aattggctca attgtacaca aatttaatat ttttaactat cccatttttt tatctatctc
     9241 tatttgaata agctaaacaa cccatttaac tgcttatttt ttagctggca catctttctt
     9301 ttcttgagtg gcttgaggtt cagctatttt acaagtacat tcgctaatac aaggaatgca
     9361 tgacatataa cgttaaaaaa ataaaacacc ctcaacgcta tcaaaaaagc tgctaataac
     9421 gaacacaaca tccaccgaat attcatcgta aaggcaaggg cttcgtaatc attcattatt
     9481 ttaatatttt tttggcaagc aaaagacagt ttcgctgcag aggcatctac agtttgccta
     9541 acggatgcgt caacctcgtc tctaatcgca tcgcttaagg ctttgcttgc acccttaatg
     9601 ccactttttt acaagatatg agatcccaag gaatggtcat ggatggccta aatggaatgt
     9661 tttaagtgga ggttccccta tgagtaacac aaatatatac ggttatttga acgcttcttt
     9721 acattatgct tattataaaa agacagctat cgaacgaacg aaacaaaaaa aatccccatc
     9781 ttttaggcta ttttttttaa agcaacccag aagaaatggc gtataaagct tatttggatg
     9841 gtcgatacta atatttacaa attagcttca tactaaaaaa gtggctaatt aaaaaatgat
     9901 gctggcaaac ctaatttgtc attttttatc ccgtggctct agacattttt cgggcatgtg
     9961 actagagaca tataaacagg attgtccata ggttataaaa atgtagacca atcaatcttg
    10021 taatttgata tcagatatag tatcattcag tatgtctaat gcaaacaaga ttttgaaaaa
    10081 aatgcgcctt aatcctaaag attggcgtat tgaggacttt gagaccgttg caaagcaaca
    10141 tggtgttaca gttcgcaagg gaggcggcag tcacgctata tttgaccacc caatgtgggt
    10201 agagttgctt tctgtacctg ctcgaaggcc aataaagcca atttatgtta aaaaatttat
    10261 tgctctaata cagattttgg agaagaatta tgaacaagat tgaatatcca tttacagtct
    10321 gcacgttacc tcaaagcgag ggtggcggtt atttaataga gtttccagat ttaccaggtt
    10381 gcatgtctga tggtgataca cttgaagaag ccattaccaa tggaagcgat gcgatggcgt
    10441 gctggatttc agccgctaag gaaataggca gagaaatccc aaatccagta gaaccacgca
    10501 ccggcaaatg ggttcaacgt gttcctaggt cattacactc acgcttagtg gcacgagcaa
    10561 aaaatgaggg cgttagtctg aatacactgg ttgtgtctat gatttcagag agtttaggta
    10621 aaagagatcc agatcatcag catcaaagac attaatgcca cccgctctct ttttcaatca
    10681 caagactcta aaggttatga tagaagtcct atcatattaa aaaaccgact ctattgatag
    10741 gcttttatca tggtttttga tccaccatta gggcgagggc taatgatagg cagcgcgcct
    10801 attatttatt gtacagaatc tgcatcggga actagaaatt ttttccagct ccctttgcaa
    10861 ataacttcac gttatatctt tgtcaaccgc gcctctaatt gatcctctac gccgcggagt
    10921 tatttgatcc cccaaaacat attagtcatc ctaatcaatt ttaaaatgta cgtcaaattt
    10981 ttcattcatt tagtgtagcg gtagcaggcc ttcgttcgcg acggtatgac gattgatctg
    11041 tacccgttac cccggacttt ttatttgata aacttagttt caataaggag tttttatggg
    11101 taccaagtta aaaagctatg acaaagaatt taaggaaaga gccattaagc ttgctttagc
    11161 ctcttcccaa ccaatttcaa aaaccgcaaa agaactgggc atgaatgaag ccacgcttta
    11221 ctcgtgggta aaaagtaacc ggaacgccta tgttgaacag catggaaaca acggaaaaga
    11281 aattcttgat gaattggcaa aacttaggaa agaaaacgcc cgtttgcggg aggaacggga
    11341 aattctaaaa aaggcggcag tcttcttcgt gaaagaagct caataaagta cgaatttatt
    11401 cagcaccatc gcgaggaatt ttgcatgaac cttatgttta aattaatggc tgtatctaag
    11461 agtggctatc acgaatagtt gtatcggggc atcagcagca gagaacaagc gaataataag
    11521 cttacaaagc agatacggtc ggtacaccag gaaagtcgca agatatatgg aggacggcgt
    11581 atttacgcag aattgcgcgc tcaagggatc tcttgcagta aaaatcgcgt tgttcgtctc
    11641 aaacgtaaag cgggaattat ggcgaagact cggcgacgat ttaaagttac aacggattct
    11701 aaacacgaac ttcccattag cggaaacata ttgaataggg aatttaatgt tttagcgcct
    11761 aacacgcatt gggtggttga tattagctac atttcaacgc tagaaggatg gttgtatctg
    11821 gcagttgtgg ttgatctgtt ttcgagaaaa gttattggtt gggagatgga agataatatg
    11881 aaaacgggtc ttgtgaaatc tgctttatta aaagcgttaa agggtcgaaa aatatcaaag
    11941 gggttagtac accattcgga tagaggcagt cagtacgcat cttttgaata tcaatctctt
    12001 ctgtcagcgc atcagattac atccagcatg agtcgtaaag gtaattgctg ggataatggt
    12061 gcgccaagaa tgacctccca aagggtggtc aatgctacat ataagatgaa gg
//
LOCUS       DAORVT010000003        10862 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_3114_length_10862_cov_3.593412, whole genome shotgun sequence.
ACCESSION   DAORVT010000003 DAORVT010000000
VERSION     DAORVT010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 10862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10862
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3114_length_10862_cov_3.593412"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PK389_00105"
     CDS             complement(<1..245)
                     /locus_tag="PK389_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22147.1"
                     /translation="MSVSSVIDKLHFSEHISKHCADFIQIGSQRLGFRTFGYRRYLRD
                     GRSFGFSNDSEWNLVFLEKFSGMPVLEYYSELSEFIST"
     gene            1044..3128
                     /locus_tag="PK389_00110"
     CDS             1044..3128
                     /locus_tag="PK389_00110"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005027476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HQY22148.1"
                     /translation="MFEVQGFIILCSILALGYGAFAARSVFHSPAGNERMREIALAIQ
                     EGANAYLNRQYKTIGMVGIVITIGLFTVLGQYSAIGFIIGAILSGLAGYIGMGVSVRA
                     NVRTAEAARTGLAKALNLAFKAGAVTGLLVVGLALLGITLYFVILQQLDLSERTITEA
                     LVSLSFGASLISIFARLGGGIFTKGADVGADLVGKIEAGIPEDDPRNPAVIADNVGDN
                     VGDCAGMAADLFETYAVTIVATMVLAGILAPSDLQETLMIYPLAIGGVCILGSIVGTW
                     FVRLGKSQNIMNALYKGLWATSVISGLLIVGLTEWLLGIQTTFEIGERTLSGLSLLYC
                     AVAGLLITGLLVWITEYYTSTKFRPVKSVAKASLTGHATNIIQGLAVSMEATALPVLV
                     ISAGILFTYVCAGLFGIAIAATTMLALAGMIIALDAYGPVTDNAGGIAEMAGLDKDVR
                     IITDALDAVGNTTKAVTKGYAIGSAALAALVLFATYTEDLSHYFPYLDINFKLQDPFV
                     MVGLFIGGLLPYLFASFGMMAVGRAGAQVVVEVRRQFREIKGIMEGTARPEYGVAVDL
                     LTKTAIREMIIPSLLPVLAPVVLYLVIKAIGGQEAAFTCLGATLLGTIVTGIFVAISM
                     TSGGGAWDNAKKYIEDGNHGGKGSDAHMAAITGDTVGDPYKDTAGPAINPMIKIINIV
                     ALLLIAIVAHAS"
     gene            complement(3256..3900)
                     /locus_tag="PK389_00115"
     CDS             complement(3256..3900)
                     /locus_tag="PK389_00115"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HQY22149.1"
                     /translation="MNMKLAGIALSGAMLVSGASFGMDFDPKFYLGAEAQANKYKGAK
                     EVKITGLGTIKNPDNKSLFGKSGAGASAFVGSQFSEYAGVELGYTVLGGAKVSYKQPG
                     FEKSSFKTKSRNMYVDVLGYVPVCEDIDLIGSLGMGRLSTKVNAKAVGPNAAGVVETT
                     NASLKSSKTGLRLGLGAQYKVTPNVGARFMVRHQKGNQFVKSVNSAGLGLFYQF"
     gene            4165..4656
                     /locus_tag="PK389_00120"
     CDS             4165..4656
                     /locus_tag="PK389_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HQY22150.1"
                     /translation="MLGKPPKVLIGWQEWCALPKLHLPAIKAKIDSGAKTSALYASDL
                     RPFHRHGELFVQFFVHPLQRTQKFETACTALVTDQRTVMNSGGHKELRYVITTTIALG
                     KMTWEIDITLTNRDPMTFRMLLGRDALKGHSIIDPGKILCQGKLMLKQLRSLYTSHKS
                     HSL"
     gene            4653..5540
                     /gene="rimK"
                     /locus_tag="PK389_00125"
     CDS             4653..5540
                     /gene="rimK"
                     /locus_tag="PK389_00125"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017293548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HQY22151.1"
                     /translation="MKIAVLTHNTNALSQRRFREAANKYGHEISFIHISYCYMNISST
                     APTVYYRNNETFENLDAIIPRINPAHSFYGTAVLRQFELMGIYTLNSSLSITWTRDKL
                     RTLQLLARKNLPLPITGFADSPEETEKLIDTVGGAPLIIRLLEGTAGRGTIFAETHQA
                     AISVINAFKQLKTNILVQECIQEAMGNDIRCIVVGNKVVATIKRSIHETGPHSLQHAT
                     KSTTLVNITPEENKMAIRAAKAMKLNLACVDLIRSNRGPLVLDVDCYPSIEMLEKMTK
                     IDIVDPIIQFLMENLKKTG"
     gene            5646..7025
                     /locus_tag="PK389_00130"
     CDS             5646..7025
                     /locus_tag="PK389_00130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HQY22152.1"
                     /translation="MKLIKWFIFFLFSISPAYLLASDLLEVYRQAQMNDPTIQAAREG
                     QLATQEAIPQARAQFLPVLNASATHLAYHQSPGTNASAPAPQTGDTHYNQSLYGLTLT
                     QPIFYYQQWIQLSTAAAQVKQANATYAAAEQDLIVRTVQGYFNVLKARDTLKFSKANR
                     SQLSKILDQTQLKFKVGLIAITDVQIAKAQYDTALAEEISNENALANQKELLREITGQ
                     PIETFSFVREDLTLKSPEPADLEKWVMTAVDQNFALEAARFDAEVSRSNIKTTEAGHL
                     PTLNINSNLNRSTSYQRTPSTTKSNVGLQVNMPLFNGGTISSKTRQAVHTYEQKQKQV
                     ETLYRKTESNTRQAYRGVLTQISQVAAQKQAIISTDSALKATEASFNVGTRTIVDVLA
                     AQTNLVQAQQNYANARYDYIIQSVLLKQAAGTLCPEEVQHINTWLIDKDVEINKSKST
                     RAITSVRPS"
     gene            complement(7003..8283)
                     /gene="waaA"
                     /locus_tag="PK389_00135"
     CDS             complement(7003..8283)
                     /gene="waaA"
                     /locus_tag="PK389_00135"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF004388.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HQY22153.1"
                     /translation="MCLNTFLRWLYSLLFTFSIPALLLRLWWKGRKNPGYRERWIERF
                     GFFKPPAQSGGLWIHAVSLGETMAAVPLIRKFQKRFPALPITLTSTTPTGYQTAKNIF
                     KDTVLQVYFPYDLAWCIKQFLKRVRPTILIVMEKELWLNCFWVCRQQKIPVFIANGAL
                     SLHSLKRYQWFSRIKQQILQCITVVFAKSETDATRFLALGLERNHLIVAGNVKFDVAL
                     KEGVEIAGKQYRETFGNRPVFIAASTHAGEEELVLLGFKQILEQQPDALLILVPRHPE
                     RFSTVAELLAQQKYTVVKRSSRFSVAPGTQVLLGDTMGELALFYAAADVAFVGGSLVP
                     VGGHNALEPAALSVPVIMGPYVDNCLEITKRLADAGALIQVSDSDALAKAVLHWFVNP
                     VDRTQAGICAKKVVEKNRGAVKQIVDVIMRGEPR"
     gene            complement(8333..8521)
                     /locus_tag="PK389_00140"
     CDS             complement(8333..8521)
                     /locus_tag="PK389_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22154.1"
                     /translation="MEIKYKGFVAEFFFFPEAGSFCGEVLNSVDLITFQAGNPQDAFE
                     AMQESVNRYLGYIVSSAT"
     gene            8539..9759
                     /locus_tag="PK389_00145"
     CDS             8539..9759
                     /locus_tag="PK389_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019621015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY22155.1"
                     /translation="MNTKDISLDIVIFGGGIAGLWTLNQLRRLGYQAILLETHTLGGG
                     QTIKSQGIIHGGMKYALSGFLSGSANTIETMPQRWKDALAGAGELDLSTVNVLSEDQL
                     LWSTGSLGSEVASFFASKALNSRIQKLPKTAYPSILKNSLFKGHAYRLEEVVLDMPSL
                     IETLTKPHSDYIFKINPNAGYAFTADPNNPQNVVSLKIFSDLQQTVNIKAKRYLFTAG
                     EGNRTLGLGLPNAPQMQVRPLQMVMVKLALPFPFFAHCIDNGTNPRITITTHPTQDGK
                     SVWYLGGQLAEDGAKRTTAEQIAYAKKEVSTLFPWLDLSGATWASFFINRAEPKQPDG
                     KRPENPFIKTLGNIMTAWPTKLALAPMLSDLILTQLKQQNILPSQTTDETKFLLARLE
                     KPAIALPPWEQYFS"
     gene            complement(9722..>10862)
                     /locus_tag="PK389_00150"
     CDS             complement(9722..>10862)
                     /locus_tag="PK389_00150"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY22156.1"
                     /translation="VALLKTFGIFLTGSLAKPLGALLFGFIGDRFGRSIALKISMLGI
                     AIPTTAIGLMPTYQDIGWIAPCLLLLCRILQGILTAGESDTVRIFIFESFFKSKPCFA
                     NSLSNVSIFVGIYLASKASAWVMAAEDPNFAWRIAFIVGGMMGAVVFWLRFTVSETVD
                     YVEHLNVRVKTRSSQSWNGFFKVFIDNKRIILTAILFCGASGGTYHFYLVFLGKYLSS
                     TLNILDSETASRFISTAVLIYTLCLPIGGFLADRFGLVRIIKTFFYGLCVALGLNIMM
                     VLAGQMPLWLMWVTAGILAIFQDPMCTLVIQRIEVGERCRCLSFGHATGSILFSGSAP
                     FISLWLWQSTENAAVPFLYCGLLASMGFLGTFLIKKNTVPTVAVQ"
BASE COUNT         3341 a         2454 c         2092 g         2975 t
ORIGIN      
        1 gtactaataa attcggaaag ctcactgtaa tattctaaga cgggcatccc actaaatttt
       61 tctaaaaaaa ccaaattcca ttctgagtca ttcgaaaatc caaagcttct gccatccctt
      121 aaataccgtc tatatccaaa tgtcctaaac cctagacgtt ggctgcctat ttgaataaaa
      181 tcagcacagt gcttgctaat atgctctgaa aaatgtaatt tatcaatgac tgatgaaaca
      241 ctcacattca acacctcaaa tgtgttacat tgaacgcctg tgtattgtac actaacagat
      301 tacaaaaata ttcttaaaaa atataaaacc ggcagttgga ctaaacaccg gattgtccct
      361 atgtctttat acaaagaatg aaacgccatt ggcacattaa agattatcag atttttcata
      421 tcatcacact ctcaaatacg ttgttaaacg gtgacttgat ccactgtggc actcattttc
      481 tcaacggctc tgctctaaaa tctatttctg atattttatc taaaggcatc cacaacaatt
      541 cgatatgcgc ttcaagcgat acgactttat gattgctttt taatgtgtct gattcaactt
      601 cgaaaatgaa attgtattcg tgattatgac aaatactgct atgccctggt tcgaagctat
      661 attctaaata tcctaaaaat ctttcttatc aaagtctttt gcaagtgcat tctaatggtt
      721 tttggcagac ttcaagttat tatcactttc agaatttatt gcacttcact gttgaagggg
      781 ccgtaggcta gcccgattgc cctactggat gacatccaat aattcagttg aggttattga
      841 ttgaaggttt ctcgccgctc aactctggtg agtctggtga gttatagtgg cgcatgacca
      901 atgtgcaata tcaccattta ccgtgaatta ctgatgttga gaacacaagg ctgagaacac
      961 aaggcttgcc cgcttttcgg tagagcgagt atacttttgg gcttagtgaa acaagcaacg
     1021 gctagaacaa aaggggcaca cagatgtttg aagtacaagg gtttattatt ttatgtagca
     1081 ttcttgcttt gggatacggt gcatttgctg cccgatctgt ttttcattcg cccgcaggca
     1141 acgagcgaat gcgggaaatt gcactggcga ttcaagaagg tgcaaatgcg tatttaaacc
     1201 gccaatataa aacgattggc atggttggca tcgtgattac cattggtctt ttcaccgtcc
     1261 tcggccaata ttccgcgatc ggctttatca taggcgcaat actctcgggt ctcgcgggtt
     1321 atatcggcat gggtgtttct gtacgagcaa atgtacgaac cgctgaagct gcaagaacag
     1381 gtcttgcaaa agcactcaat ttggctttta aagcaggcgc tgtgacaggg cttttagttg
     1441 taggcttagc acttttaggc attacgcttt atttcgttat tttacaacaa ttggatttat
     1501 ctgaacgcac aataaccgaa gcgctggttt ccttaagttt cggcgcttca ttaatttcta
     1561 tcttcgcacg tctcggcggc ggtatcttta caaaaggtgc agatgttggc gccgatttgg
     1621 tcggaaaaat tgaagccggt attccagaag atgacccacg aaaccctgct gttattgctg
     1681 ataacgtggg tgacaatgtc ggcgattgcg ctggcatggc ggctgatttg tttgaaactt
     1741 atgccgttac cattgtcgcc acaatggttt tagcaggcat tttagccccc agtgatttgc
     1801 aagaaaccct tatgatttat ccgctcgcga ttggcggtgt gtgtatttta ggttctattg
     1861 taggtacttg gtttgttcga ttgggcaaaa gccaaaacat tatgaacgcc ctttacaaag
     1921 gactatgggc aacaagtgtt atttccggat tattaattgt cggtcttacc gaatggctgc
     1981 tgggcattca aaccaccttt gaaatcggtg aaagaacgct ttctggttta agtttgttat
     2041 attgtgctgt agcaggattg ctgatcacgg ggcttttggt ttggatcacg gaatattata
     2101 cctcgaccaa atttagaccc gttaaaagtg ttgcgaaggc gtctctaaca ggacatgcca
     2161 ccaacattat tcaaggatta gcagtttcga tggaagcgac tgcattaccc gtgttggtga
     2221 ttagcgctgg aatcctcttt acctatgtat gtgcaggctt attcggcatt gccattgcag
     2281 cgaccaccat gttggcgctc gcaggtatga ttattgcact tgatgcttat ggtcctgtaa
     2341 cagacaacgc aggcggtatc gctgaaatgg ccggcttaga caaagatgtt cgcatcatta
     2401 ccgatgcact cgatgcggtt ggtaatacaa ccaaagccgt taccaaaggg tatgccattg
     2461 gttctgctgc attggctgcc ttagtcttat ttgcaaccta tacagaagat ctgagccatt
     2521 actttccgta cttggatatt aactttaagt tacaagatcc gtttgtgatg gttggtttat
     2581 ttatcggtgg cttattgccc tatttgtttg cctctttcgg aatgatggcg gttggacgcg
     2641 caggcgcaca agtggtcgta gaagtacgtc gtcagtttcg cgagattaag gggattatgg
     2701 aaggcactgc aaggcctgaa tacggtgttg cagttgatct cttaacaaaa acagcgattc
     2761 gcgaaatgat tattccttct ttattacccg tacttgcacc ggttgtttta tacctcgtta
     2821 taaaagctat cggtggacaa gaagcggcgt ttacttgctt aggtgctacc ttactcggaa
     2881 ctattgtcac gggtattttt gtcgcaatct cgatgacttc gggcggtggc gcttgggata
     2941 acgcgaagaa atatattgaa gatggaaatc atggtggaaa aggatcagat gcgcacatgg
     3001 ctgctattac cggtgatacc gtgggtgacc cttacaaaga caccgcaggc cctgcgatta
     3061 atcccatgat taagattatc aacatcgttg cattgctatt gattgcgata gttgctcacg
     3121 cttcttaggt tgttaaagcc cccttcatag ggggccgatg tcgccgagcg atagcgaggg
     3181 gatatcgggg ggatgtacaa aaaccaaggg cccttacggg cccttgctac acagtcaaaa
     3241 tggttttttt gttaattaaa attgataaaa aagacctaaa ccagcggagt ttacactctt
     3301 cacaaactga ttaccttttt gatgacgaac cataaagcgt gcaccaacat ttggtgtaac
     3361 tttatactga gcacccaaac caagacgaag tcctgtttta gaagatttca gcgatgcgtt
     3421 cgtagtctcc acaacacctg cagcatttgg gcctaccgct ttagcattca cttttgtaga
     3481 caaacgtccc atacccagtg aaccaatcaa atcaatgtct tcacaaacgg gaacataacc
     3541 taaaacatct acatacatgt tacggctctt ggttttgaat gaagattttt caaacccagg
     3601 ttgcttataa gatactttag caccacctag cacagtgtat cccagttcaa cgcccgcata
     3661 ttccgagaat tgcgaaccaa caaatgcact tgcaccagca ccggattttc caaagagtga
     3721 tttgttatca ggattcttta ttgttcctaa tccagttatt ttaacttctt tcgcaccttt
     3781 gtacttattc gcttgcgctt cagcacccag atagaatttc ggatcgaaat ccataccaaa
     3841 agacgcacca ctaacaagca tggcacctga caaagctata ccagctaact tcatattcat
     3901 attacttcct ccaaccattt tgatgcatag tttcaaacta tgagccacga tggtaataga
     3961 ctttcgttgt aaggtaaata cttttcaact aaaaaaaatg aaaataaagc caacaattct
     4021 gttttaacaa ccagtaattt agcatttttt aatagaaaac taatttttta cacagtggtc
     4081 aatatattgc gtgtatgtca taaacagtca ttgcaagcaa ccctaagata acctaacatt
     4141 gcaattggag gactggctta aattatgttg ggaaaaccgc ctaaagtact tatcggatgg
     4201 caagaatggt gtgcgcttcc caagctccac ctgcccgcta ttaaggccaa aattgattcg
     4261 ggtgcaaaaa cctccgccct ctatgcttcc gatcttcgtc cttttcatcg tcatggcgaa
     4321 ttatttgttc aatttttcgt ccatccgcta caacgaacac aaaaattcga aacggcctgt
     4381 accgcgttag tcaccgatca acgaaccgtt atgaattctg gtgggcataa agaactgcgt
     4441 tatgtgatta ccacgacgat tgctttaggc aaaatgacct gggaaattga tattacatta
     4501 acaaaccgtg accccatgac ttttcgtatg ctattaggaa gagatgcgct gaagggtcat
     4561 tccatcattg atccgggtaa aattttgtgt caaggaaaac tcatgctaaa gcagcttcgc
     4621 tcactctaca catcccacaa gagccattcc ctatgaaaat tgcggtatta acacataaca
     4681 caaatgcttt aagccaacga cgtttcagag aagcggccaa taaatacggg cacgaaattt
     4741 cttttattca tatttcttat tgttatatga acatttccag tacagcgcca acggtttatt
     4801 atcgtaataa cgaaactttt gaaaacttag atgcgattat tccccgcatt aatccagcgc
     4861 acagttttta cggtaccgct gtcttaagac agtttgaatt aatggggatc tacacgctta
     4921 atagttctct ctctattact tggacacgag acaaactcag aacactacaa ttgcttgcac
     4981 gcaaaaatct tccgctaccc attaccggtt ttgcagattc gcctgaagaa accgaaaaat
     5041 taattgacac cgtcggtggc gcacccctaa ttatacggtt actagaaggc actgccggtc
     5101 gtgggactat ttttgctgaa acacatcaag ccgctatcag tgtcatcaac gcttttaaac
     5161 aattaaaaac gaatatacta gtacaagaat gtattcaaga ggcgatgggt aacgatatac
     5221 gttgtattgt ggttggcaac aaagtcgttg caaccattaa gcgatctatt catgaaacag
     5281 ggccacattc tctgcaacac gccactaaat ctacgacgct agtcaatatt acacccgaag
     5341 aaaataaaat ggctattcgc gctgctaagg caatgaaact gaatttagcc tgtgttgatt
     5401 taatacgatc caatcgcgga ccactcgtat tggatgttga ctgctatccc agtattgaaa
     5461 tgctagaaaa aatgacaaaa atcgatattg ttgatccgat tattcaattc ctgatggaaa
     5521 acctaaagaa aactggttaa tctttagcca ttgtaatgca ataacgaagg tgaaattgtg
     5581 gtgcccatga cagactcatg ttatacttcc agatacaatg ttagaatggt gaatttattc
     5641 aagctatgaa actaataaaa tggtttatct ttttcctttt tagcatttcc cccgcatacc
     5701 tcttagcctc agatctttta gaggtttacc gtcaggcaca gatgaatgat cccactattc
     5761 aagcagcaag agaagggcaa cttgctacac aagaagcaat cccccaagca cgcgcgcaat
     5821 ttttgcccgt actcaatgca agcgcaaccc atcttgccta ccatcaatct ccgggaacca
     5881 acgcctcggc cccagctcct caaactgggg atactcatta taatcaaagc ctctatggcc
     5941 ttacgctcac gcaacccata ttctattatc aacaatggat tcaactttca acagcagcag
     6001 cacaagtcaa acaagccaat gccacctatg ctgctgctga acaagactta atcgtgcgga
     6061 cagtacaggg gtactttaat gtactcaaag cgcgcgatac gttaaaattt tcaaaagcga
     6121 accgaagcca actctccaaa attttagacc aaacacaatt aaagtttaaa gtcgggctta
     6181 tcgctattac cgacgtacaa atagccaagg ctcagtatga cacagcttta gcagaagaaa
     6241 tttcgaatga aaatgcactc gccaaccaaa aagaactctt gcgagaaata acaggacagc
     6301 ctatcgaaac cttttctttt gtacgtgaag atttaacctt aaaatcacct gaacccgcag
     6361 atcttgaaaa gtgggtcatg acagctgtgg atcaaaattt tgcattagaa gcggcgcgct
     6421 ttgatgcaga ggtttcgcga tccaatatta agacgaccga agcaggacac ttgcccacgc
     6481 tcaatatcaa tagcaatctt aaccgatcaa caagctatca aagaacgccc agcactacaa
     6541 aatcaaatgt tggattacaa gtaaatatgc ctttatttaa cggtggcact atctcctcaa
     6601 aaaccaggca agccgtacac acctatgagc aaaagcaaaa acaagtagaa accttgtatc
     6661 gcaaaacaga aagtaacact cgacaagcgt atcgcggtgt attaacgcaa atcagccaag
     6721 ttgccgctca aaaacaagcg attatttcta ccgatagcgc actaaaagct actgaggctt
     6781 cttttaatgt aggaactcgt acaattgtag atgttttagc agcacaaacc aatttggttc
     6841 aagcacaaca aaattatgcg aatgcacgct atgattacat catacaaagt gttttattaa
     6901 aacaagccgc tggtacttta tgtccagaag aagtgcagca tattaatact tggcttattg
     6961 ataaggatgt tgagatcaat aaatcaaaat caacaagggc gattacctcg gttcgcccct
     7021 cataatgaca tcaacaatct gctttacagc accccgattt ttttcaacaa ctttttttgc
     7081 acatatgccg gcttgtgtgc gatcgactgg atttacgaac cagtgcaata ccgcttttgc
     7141 caatgcatcg ctgtccgata cctgaatcaa agcaccggca tctgctagcc gttttgtaat
     7201 ttccagacaa ttatcaacat aaggtcccat aataacagga acacttaaag cagcgggttc
     7261 taatgcatta tgaccaccga ctggaacaag acttccaccc acaaaagcaa catcggcagc
     7321 agcataaaaa agtgctaact cacccatcgt atcgcccaat aaaacctgcg ttcctggtgc
     7381 tactgaaaat cggctgctgc gttttacgac agtatacttc tgttgtgcta acagctccgc
     7441 aaccgtgcta aagcgttctg gatgtcgagg cactaatatt aataatgcgt ctggctgttg
     7501 ttctaagatc tgcttgaacc ctaataacac gagctcttct tcaccagcat gcgtgctggc
     7561 agcgataaaa acagggcgat tgccaaaagt ctctctgtat tgttttccag caatttccac
     7621 gccttcttta agagccacat caaacttcac attgccagca acaataagat gattgcgttc
     7681 taatcctaat gctaaaaatc ttgtggcatc tgtttcagat tttgcaaaaa caaccgtaat
     7741 acattgcaat atttgctgct ttatgcgaga aaaccattga taccttttaa gggaatgtag
     7801 cgaaagcgca ccattcgcaa taaaaacagg tattttttgc tgacgacaaa cccagaaaca
     7861 attgagccaa agttcctttt ccataacgat taagatcgtt ggacgaacgc gttttaaaaa
     7921 ttgcttaata caccaagcta aatcataggg aaaataaacc tgcaagacgg tgtctttaaa
     7981 aatatttttc gccgtttgat atcccgtagg tgttgtcgaa gtcaatgtaa taggcagtgc
     8041 gggaaaccgt ttttgaaatt tccgaattaa gggaactgcg gccatggttt ctcccaaaga
     8101 aacagcgtgt atccacaacc cgcccgattg tgcagggggc ttaaaaaagc cgaagcgctc
     8161 gatccaacgc tctctatagc ctggattttt gcgacctttc caccaaagcc taagcaataa
     8221 agctggaata ctgaatgtaa aaaggaggct atataaccaa cgtaaaaatg tatttaagca
     8281 catagatgct gatcatcgca tttaaaacac tgcaagtcga taatcgaaca gcttaagtcg
     8341 cagagcttac tatataacct aaatagcgat taaccgattc ttgcatagcc tcaaatgcat
     8401 cttgtggatt tcccgcctga aaggtaatca aatcgaccga attcaatact tcaccacaaa
     8461 atgaaccggc ttctgggaag aaaaagaact cggcaacaaa gcccttatat ttaatttcca
     8521 ttttgatata atatttccat gaatactaaa gacatatcac tcgatatcgt tatttttggc
     8581 ggcggtattg ccgggctctg gactctcaat cagctacgcc gccttggcta tcaggcaatt
     8641 ttactagaaa cgcatacact cggtggcggc caaacgatta aatcgcaagg tatcattcat
     8701 gggggcatga aatatgcgct ctctggcttc ttaagtggct cagcaaacac catcgaaacc
     8761 atgcctcaac gatggaaaga tgctttagca ggtgctggag aactcgattt aagcaccgtt
     8821 aatgtactct cagaagatca attattgtgg tccaccggca gcttagggtc agaagttgcc
     8881 agtttctttg ccagcaaagc attaaacagc cgtattcaaa aactgcccaa aacggcttac
     8941 ccgtccattt taaaaaattc gctttttaaa ggtcatgcct atcgcttaga agaagttgtt
     9001 ctcgatatgc cttctttaat tgaaacattg acaaaacccc atagtgatta catttttaaa
     9061 ataaacccaa atgcaggata cgcttttaca gctgatccaa acaatccgca aaatgtcgta
     9121 tctcttaaaa ttttttcaga tcttcaacaa acggtaaaca taaaagcaaa acgctatctg
     9181 tttacagcgg gcgagggcaa tagaacgctc ggcttaggcc tgccaaatgc acctcaaatg
     9241 caagtccgtc cactgcaaat ggtcatggtt aaactggcgt tgcctttccc gttctttgca
     9301 cactgtatag ataacggaac caatcccaga atcactatca ccacgcaccc cacccaagat
     9361 ggcaaaagcg tttggtattt agggggacaa cttgcagaag atggcgcaaa gcgaacgaca
     9421 gcagaacaaa ttgcctatgc taaaaaagaa gtgagcactt tatttccatg gttagatctt
     9481 tcaggtgcaa catgggctag cttttttatc aatcgcgcag agcctaaaca acccgatgga
     9541 aagcgtccag aaaatccttt tataaaaaca ctgggcaata taatgactgc atggccaacc
     9601 aaactcgcac ttgcccccat gctatctgat ctcattctaa cgcaattaaa acaacaaaat
     9661 attctgcctt cacaaacaac ggatgaaaca aaatttctgc tggctcgctt agaaaaaccc
     9721 gctattgcac tgccaccgtg ggaacagtat ttttcttaat caaaaaagtg cctaaaaaac
     9781 ccatcgatgc taacaatcca caatataaaa atggcactgc ggcgttctct gtgctctgcc
     9841 acaaccacaa gcttataaaa ggcgcactac cggaaaacaa tattgatcct gtcgcatgac
     9901 caaaacttaa acaacgacac cgttctccga cctctattct ctggatcaca agggtacaca
     9961 tcggatcttg gaaaatagct aaaatgccag ccgttaccca cattaaccat aacggcattt
    10021 gtcctgctaa taccatcatg atatttaaac ctaaagcaac acataatcca taaaaaaaag
    10081 tctttatgat tcgaaccaag ccaaagcgat cggctaaaaa ccctcctatc ggcagacata
    10141 atgtataaat taaaacagcc gttgaaataa aacgcgaggc tgtttctgaa tctaaaatat
    10201 tcaaggtgct gcttagatat tttcccaaaa atactaaata aaaatgatag gttccacccg
    10261 atgcgccaca aaaaagaatc gccgttaaaa ttatccgctt attgtctata aaaactttaa
    10321 aaaaaccatt ccacgactgt gaacttcgtg ttttaactcg tacattaaga tgttctacat
    10381 agtctaccgt ttcagagact gtaaagcgca accaaaaaac aacagcgccc atcataccgc
    10441 ccacaataaa agcgatacgc catgcaaaat ttggatcttc cgctgccatt acccatgccg
    10501 atgccttcga tgccaaatag atacccacaa atatcgaaac attcgataga ctattggcaa
    10561 agcagggttt ggacttaaaa aaagattcaa aaataaaaat gcgaacggta tccgattccc
    10621 cggcggttaa aatcccttgc aagatccgac acagtaataa taaacacggc gcaatccaac
    10681 caatgtcctg ataagtcggc attaatccaa tggcggttgt cggtatcgcg atacccagca
    10741 tactgatttt taatgcaata ctacgaccaa agcggtcacc aataaaacca aaaagtaatg
    10801 cacccaatgg ttttgctaaa gatccggtta agaaaatacc aaaggttttg agcaacgcaa
    10861 cc
//
LOCUS       DAORVT010000004         9919 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_3629_length_9919_cov_4.099655, whole genome shotgun sequence.
ACCESSION   DAORVT010000004 DAORVT010000000
VERSION     DAORVT010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9919
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3629_length_9919_cov_4.099655"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..86)
                     /locus_tag="PK389_00155"
     tRNA            complement(11..86)
                     /locus_tag="PK389_00155"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51..53),aa:Asn,seq:gtt)
     gene            complement(131..1324)
                     /locus_tag="PK389_00160"
     CDS             complement(131..1324)
                     /locus_tag="PK389_00160"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008735159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HQY22157.1"
                     /translation="METQLSDRVQRIKTSATLAMATKAAALKKQGINVIDLSTGEPDF
                     DTPEHIKQAAIAAIDRGFTKYTAIGGVLELREAIVQKFNRDNQLNYAPNQILVSNGGK
                     QSFYNLAAALLNPGDEVIIPAPYWVSFPDMVLLSDAKPIIAFAGIDDNFKISPAQLEK
                     VITSKTRLLVLNSPCNPTGVTYTHQELVALGKVLAKHPNILIATDDMYEKIRWGTEPF
                     CNILMACPDLYERTLVLHGVSKTYAMTGWRIGFAAGPVKIIQAMTSIQSQSTSNPCSI
                     SQMAAKAALEGDQTCVATMVDAFKRRHDYFIPALNTIEGMECRLGNGAFYAFPKVEGL
                     MKRLGLKDDLALAEFLLTEAAVAVVPGTPFGAPGYIRLSFATSLDNLQETIKRLQAID
                     PKIGS"
     gene            1404..3419
                     /gene="uvrB"
                     /locus_tag="PK389_00165"
     CDS             1404..3419
                     /gene="uvrB"
                     /locus_tag="PK389_00165"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HQY22158.1"
                     /translation="MSDQFQLSAKFKPAGEQPEAIAKLVDGLNSGLMHQTLLGVTGSG
                     KTFTIANAIAQSNRTTLVMAPNKTLAAQLYGEMKEFFPSNAVEYFVSYYDYYQPEAYV
                     PASDTYIEKDASINEHIEQMRLSATKALLERPDTIIVATVSAIYGLGDPKSYLSMVLH
                     LDRGDKIDQRTLLRRLAELQYSRNDIDFRRTMYRVRGDVIDVFPAESDNDAVRIELFG
                     DEIENISIFDPLTGEILKRVPRITIYPKTHYATPRQRILDAIEEVKLELRERLADLES
                     ENKLVEAQRLEQRTRFDIEMMQELGYCSGIENYSRYLSGRKAGEAPPTLMDYLPSNAL
                     LIIDESHVTIPQLGGMYKGDRARKENLVNYGFRLPSALDNRPMRFDEFEKRTPQSIFV
                     SATPSDYELRHSESVIELIVRPTGLIDPEIEMRPASTQVDDVLSEIKKRTVVHERVLI
                     TTLTKRMAEDLTHYLVEQGIKVRYLHSDIDTVERVEIVRDLRLGVFDVLVGINLLREG
                     LDLPEVSLVAIFDADKQGFLRSERSLIQTIGRAARHLNGKAILYADIVTNAIQRATEE
                     TDRRRTIQRQYNAEHGITPTSIKKAVQDILEGAYAGELKSGKSGRHTKEMRLEEERNK
                     KLSPKQLAAKIQALENAMYTHAKNLEFEAAARVRDEIHKLNECFVKN"
     gene            3662..4150
                     /locus_tag="PK389_00170"
     CDS             3662..4150
                     /locus_tag="PK389_00170"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HQY22159.1"
                     /translation="MNHNTTSYQEINGIDCCFNLRKVTRAVTQFYDRHLEPADIRSTQ
                     FNLLLTLSASAGKTLTEMAEGLVMDRTTLTRNLKPLEKSALITTTKLSDRRTKGYVLT
                     EKGRLAIEKGVPLWQKAQQQITGQLGEDRYKRLLEELKVVRSFANTPKVTRHKDLSNK
                     LI"
     gene            4235..5287
                     /gene="recA"
                     /locus_tag="PK389_00175"
     CDS             4235..5287
                     /gene="recA"
                     /locus_tag="PK389_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HQY22160.1"
                     /translation="MDEHKKKALNAALSQIEKQFGKGAVMLMGDGSPVANIQAISTGS
                     LGLDIALGIGGLPKGRVVEIFGPESSGKTTLTLQVIAQCQKMGGTAAFVDAEHALDPS
                     YAEKLGVKIDELLVSQPDTGEQALEIVDMLVRSSAVDVVVIDSVAALTPKAEIEGEMG
                     DQHMGLQARLMSQALRKLTANIKRSNTLVIFINQIRMKIGVMFGSPETTTGGNALKFY
                     ASVRLDIRRTGAIKKGEEILGNETRVKVVKNKVAPPFKQAEFEILYGQGTCLLGEIIE
                     LGVKLNLIDKSGAWYSYKGNRIGQGKENVRQHLQENKAIADEIEKAIRNTLLVPMPVG
                     SKAALVEEDLALALED"
     gene            5297..7951
                     /gene="alaS"
                     /locus_tag="PK389_00180"
     CDS             5297..7951
                     /gene="alaS"
                     /locus_tag="PK389_00180"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007145407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HQY22161.1"
                     /translation="MNTEQLRQTFLDYFEKHGHTIVPSSSLVPEDSTLLFTNAGMVQF
                     KDIFLGSKTRSDTRAASSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKR
                     EAMQFAWDFLTKVLKLPANRLWVTVFTEDSEAENIWLNDIKIDPKRFSRCGAKDNFWS
                     MGDTGPCGPCTEIFYDHGSEIAGGPPGSIDEDGDRYVEIWNLVFMQYNRFEDGTLSPL
                     PKPSVDTGMGLERIAAVVQGVHSNYDIDIFKKLIQSIIEIGNIQDTKNQSLRVIADHI
                     RSCAFLIVDGVLPSNEGRGYVLRRIARRAIRHGHRLGFTDPFFYRLVKPLAQVMGEAY
                     PELIEKISLIERALQQEEVQFNRTLDQGLKILENDLTNITGNLIPGETVFRLYDTYGF
                     PVDLTNDIARERGLTIDMAGFNESMEAQKTRARESSQFGVNYNDHTIPTHNHTTDFVG
                     YDSLKAECLIVGLYQDGKAVSELQEGEAGAVVLKHSPFYAESGGQLGDQGVFTFKEGI
                     FEVQNTRKVGDAHFHHGIVKKGVFSIDDQILASVDHLKRNATALNHSATHLLHAALRQ
                     IIGTHVQQKGSLVEPDRLRFDFSHFEALSREQITAIERLVNEKIRDNTAVETEVLPIE
                     EAIANGAMALFGEKYGKAVRVLHMGSDFSVELCGGTHVKHTGDIGVFKITAEYGIASG
                     IRRLEALTGARGLDWMNAIEAQRDRLALFLKCKPETLEDKLQQLAQKNRQLEKDLQTL
                     KALMMTGKAHDLEKVLEEKIQKTPHGMRVLAAKLPVGDIKTLRETVDHWKSKLGSRAV
                     IVLGGINDQNRVCLVAGTTRDCQEQFKAQELVNIVAAEVGGKGGGRSDMAEAGGTQPE
                     HLDKALGLVYDWVSKTESLSTNINHPMA"
     gene            7999..9246
                     /locus_tag="PK389_00185"
     CDS             7999..9246
                     /locus_tag="PK389_00185"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011169154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HQY22162.1"
                     /translation="MALIVQKFGGTSVGSIECIRQVAEKVIKTREAGHDLVVVVSAMA
                     GETDRLVRLAQELSQLPNPREFDVLLSTGEQVVIALLSLALLERGCPARSYTGTQVHI
                     RTDSAHNKARILGIDGSKILSDLKAGRVVVVAGFQGTDEMGNITTLGRGGSDITAVAL
                     AAALDADECQIYTDVDGVYTADPKIVADAGRMSQVTVEEMLEMASNGAKVMQIRAVEF
                     AGKYHVPVRVLSTFQEGTGTLIIADEANMQHSAVSGIAFNQDEAKLVIYGVPNKPGIA
                     AHILGSMSDVHIEVDMIVQTQTEEEKTDLAFTVHKRDFQQALLILENVAKAINAKHIT
                     SDAHVAKLSLVGVGMRTHAGVASKMFQVLGKEGINIQLISTSEIKISVIINEQYLERG
                     VRALHDAFQLGTEERKTKEIEQC"
     gene            9240..9476
                     /gene="csrA"
                     /locus_tag="PK389_00190"
     CDS             9240..9476
                     /gene="csrA"
                     /locus_tag="PK389_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HQY22163.1"
                     /translation="MLILTRRVGETLIVGDEVTVTVLGVKGNQVRIGVNAPKNVSVHR
                     EEIYQRIQREKQDGIVHEKADQGSNDVEQDESDH"
     gene            9607..9697
                     /locus_tag="PK389_00195"
     tRNA            9607..9697
                     /locus_tag="PK389_00195"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9641..9643,aa:Ser,seq:gct)
BASE COUNT         2926 a         1851 c         2394 g         2748 t
ORIGIN      
        1 gctttgtagt tggctccccg agacggactc gaaccgccga cctagtggtt aacagccacg
       61 tgctctacca gctgagctat cggggaatgg cgtttagaac agtgatagta ccgaacttaa
      121 tgggggatgg tcaactccca atcttagggt ctatggcttg gagccgcttg atagtttctt
      181 gtaagttatc taagctagtt gcaaacgaca gtctgatgta tcctggcgcc ccaaaaggtg
      241 tacctggtac tacggcaact gctgcctcag tcaacagaaa ctccgcaagc gccagatcat
      301 ccttcaaccc caaacgcttc atcaatccct cgacctttgg aaaagcatag aacgcgccat
      361 tgccgagacg gcattccatg ccctcaatgg tattcagtgc aggaataaaa taatcatgtc
      421 gccttttaaa ggcgtctacc atggtggcta cacaggtttg atctccctct agtgccgcct
      481 tagcagccat ttgagagata gaacaaggat tagaagtact ttggctttga atgcttgtca
      541 tcgcttggat gatttttaca ggacctgcag caaagccaat gcgccagcct gtcattgcat
      601 aggttttaga aacaccatgc agtacaaggg ttctttcata taaatccggg caagccatta
      661 agatattgca aaaaggttct gtaccccagc ggattttttc atacatatcg tctgttgcaa
      721 tcaggatgtt cggatgctta gccaaaacct ttccaagtgc aacaagttct tgatgcgtat
      781 acgtcacacc cgttggatta caagggctat ttaagactaa aagtcgcgtt ttagaggtga
      841 tcactttttc gagttgtgcc gggctaatct tgaaattatc atcaatgccc gcaaaagcga
      901 tgataggttt tgcatcacta agtagtacca tatctggaaa agaaacccaa tagggtgcag
      961 ggataatcac ttcatcacct ggatttaaca acgctgcagc aaggttataa aaactttgtt
     1021 taccgccgtt ggagactaaa atttgattgg gtgcataatt taattgatta tcacgattaa
     1081 atttttgaac aatcgcttct cgaagctcta aaaccccacc tatagcagtg tactttgtaa
     1141 atccacggtc aatcgccgca atcgctgctt gtttaatatg ttccggtgta tcaaaatcag
     1201 gctctcccgt actgagatcg ataacattta ttccctgttt tttaagggcg gcggccttgg
     1261 ttgccattgc aagtgttgcg gatgtcttga tgcgttgtac gcgatcagaa agttgtgttt
     1321 ccaaaagatg ttccttatgt gatatagtga gccaatatat ataacattaa tacacgctta
     1381 gcccaagtac acaggtgatt tttatgtctg atcagtttca attatcggct aaatttaagc
     1441 ctgcggggga gcagcctgag gctatcgcaa agttggtgga tggactaaat tccgggttaa
     1501 tgcaccaaac gctcttaggt gttacaggtt cgggtaaaac ttttaccatt gcaaatgcga
     1561 ttgcacaaag caatcgtaca acacttgtga tggcacccaa caaaacgctt gccgcacaac
     1621 tgtacggtga gatgaaagaa tttttcccca gcaatgcggt tgaatatttt gtttcttatt
     1681 atgattatta ccagcccgaa gcctatgtgc ctgcttcaga tacttatatc gaaaaagacg
     1741 cgtctattaa tgaacatatt gaacaaatgc gtttatcagc gacgaaagct ttgttagaac
     1801 gacccgatac gattattgtt gcaacggttt ctgccattta tggtttaggt gatccgaaat
     1861 cttatctttc gatggttctg catttagatc gcggtgataa aattgatcaa cgaaccttgt
     1921 taagacgttt agcagaatta caatattcac gcaatgatat tgattttagg cggacgatgt
     1981 accgagtgcg cggggatgtg attgatgtat tccctgcgga atctgacaat gatgcggtta
     2041 ggattgaact ctttggcgat gaaattgaaa acatcagtat ttttgatccc ttaacgggtg
     2101 agattttaaa gcgtgttccg cgtattacta tttatcccaa aacccattat gcaacaccca
     2161 ggcagcgtat attagacgcc attgaagagg ttaaactgga actcagagaa agattggccg
     2221 atctggaatc cgaaaacaaa cttgtggaag cgcagcgttt agaacaaaga acgcgttttg
     2281 atattgaaat gatgcaagag ctggggtatt gctctggcat agaaaattat tcgcggtatt
     2341 tgtcgggtcg gaaagcgggc gaggcgccac ctacgctcat ggattatttg ccgagcaacg
     2401 cgttattaat tattgatgaa tcccatgtga cgattccgca attaggaggc atgtataaag
     2461 gcgatagggc gcgcaaagaa aatttggtga actatgggtt tcggttacct tctgctttag
     2521 ataatcgccc gatgcgtttt gatgaatttg aaaaacgaac gccccaaagt atttttgttt
     2581 ctgcaacacc cagtgattat gagttgcgac attctgagag tgtaatcgaa ctgattgttc
     2641 gtccaacggg gttgattgat cctgaaatcg aaatgcgccc tgcttctaca caggtggatg
     2701 atgtattgtc cgaaatcaaa aaaagaacgg ttgtgcatga gcgcgtctta atcaccactt
     2761 taaccaaaag aatggcagaa gatctcacgc actatctggt agagcaaggg atcaaagtgc
     2821 gttatttaca ttcagatatt gatacggtcg aacgtgtaga gattgttcgt gacttacgtt
     2881 taggtgtttt tgatgtatta gtaggcatta atttattacg cgaaggatta gatctgccag
     2941 aagtgtcgtt ggttgctatt tttgatgcag acaaacaagg atttttgcga tcggagcgtt
     3001 ctttaataca aaccattggt cgtgcggcgc gtcatctgaa tggaaaagca attttatatg
     3061 cagatatcgt cacgaatgcg atacaacgtg cgacagaaga aacagacaga cgacgtacga
     3121 tacaacgaca atataatgcg gaacatggta ttactccgac cagtatcaaa aaagccgtac
     3181 aagatatttt agaaggcgct tatgcagggg aattaaaatc tggaaaatcc ggtcgccaca
     3241 ctaaagagat gcgattagaa gaagagcgta ataaaaaatt gtcacccaaa caattggcgg
     3301 caaagatcca agcgctcgaa aacgccatgt acacacacgc aaaaaattta gagtttgaag
     3361 cggcggcgcg tgtgcgggac gaaattcata aactcaatga atgttttgta aagaattgag
     3421 tttttccttg tagcgcctgt gttgccataa aattctattt ttgaatgggc taatggttac
     3481 ccaggctttg tagtaaaaaa atagaagcaa aaaaactgat tcaaaagcca ggcatattaa
     3541 ctgaaattag cggttgaggc gtctcttggg tttggtaaaa ttttaaagta aaaatattta
     3601 gttttcttcc ttgtattttg atataaagag agcctacact tttgttagga ggtgcactaa
     3661 catgaaccat aacacaacat cctaccaaga gatcaacggc atagactgtt gttttaactt
     3721 aagaaaagtg acccgtgctg ttacacagtt ttatgatcgt cacttagagc cagcagacat
     3781 acggtcaacc caatttaact tattattgac cttgtcagcc tctgccggta aaacacttac
     3841 tgaaatggcg gaaggattgg tgatggatcg cacaacccta acccgcaatt taaaaccctt
     3901 agaaaaatcg gcattgatca cgacgacgaa attgtcggat agacggacta aggggtatgt
     3961 tttaactgaa aagggtcgat tggctattga aaaaggtgtt ccactttggc aaaaagcaca
     4021 acagcagatt acgggccagc tgggagagga tcgttataag cgattactcg aagagttgaa
     4081 agtagttcgt tcttttgcga atacgccgaa agttacgcga cacaaagacc tctctaataa
     4141 actcatataa ataacctatg ttttaaacgg tgtcgaagat ccgctgagtt tatgcgatac
     4201 ttatatttcg tatttattaa gcaggggatc taaaatggat gagcataaga aaaaggcact
     4261 caatgccgct ttatcgcaaa ttgagaagca gtttggcaaa ggggctgtga tgttaatggg
     4321 cgatggtagc ccagttgcga atatccaagc catttcgacc ggttccctgg gactcgatat
     4381 tgcgttgggc atagggggat tacccaaagg acgcgttgtt gaaatttttg gacccgaatc
     4441 ctccggaaaa accacactca cattacaagt gatagcgcaa tgccaaaaaa tgggtggcac
     4501 cgcagccttt gtggatgccg agcatgcact ggatcccagt tatgcagaaa aactgggtgt
     4561 taagatcgat gaactgctcg tctcacaacc cgatacgggc gaacaagctt tagaaatcgt
     4621 cgatatgtta gtgcgttcga gtgccgtgga tgttgtggta atcgactccg tggccgcgtt
     4681 aacacccaaa gcagaaattg aaggcgaaat gggtgatcaa catatgggct tacaggctcg
     4741 attgatgtcg caagcgctac gcaaattaac ggccaatatt aaacgttcga atacattggt
     4801 tatttttatt aatcagatcc gcatgaaaat tggcgtgatg tttggtagtc ccgaaacgac
     4861 aactggcgga aacgctctga agttttatgc ctctgtacga ttagatatcc gtagaacagg
     4921 tgccattaag aaaggcgaag aaatcttagg caacgaaact cgggttaaag tggttaaaaa
     4981 taaagtggct ccgcccttta aacaagcaga gtttgaaatt ttgtatggcc aaggcacgtg
     5041 tctattaggt gaaattattg aattgggcgt taagttgaat ttaattgata aatctggcgc
     5101 gtggtatagc tataagggca atcgtattgg acaaggtaag gaaaatgttc gacagcactt
     5161 gcaagaaaac aaggcgattg cagatgagat tgaaaaagct atccgtaata ccttgttggt
     5221 acctatgcct gttggatcta aagcagccct ggttgaagaa gatcttgcgt tggctctgga
     5281 agattaaggg ctgattatga ataccgagca gttgcgtcaa acgtttttag attattttga
     5341 aaagcacggg cacaccattg taccgagtag ttctttagtc cctgaggact cgacattgtt
     5401 atttaccaat gcgggcatgg tgcagtttaa agatatcttt ttaggatcga aaacgcgatc
     5461 tgatacacgt gccgcgagta gtcagcggtg cgtgcgtgcg gggggaaaac acaatgacct
     5521 cgaaaacgtt ggatacactg ctcggcatca tacctttttt gagatgctcg ggaactttag
     5581 ttttggggat tattttaaac gtgaagccat gcagtttgca tgggattttt taacaaaagt
     5641 tcttaaattg ccggcgaatc gtttgtgggt aacggttttt acggaagaca gcgaagctga
     5701 aaacatttgg ttaaacgaca ttaaaataga tcccaagcgt ttttcgcgtt gtggcgccaa
     5761 agataatttt tggtctatgg gagacacagg cccttgtggc ccttgtacag aaatttttta
     5821 tgatcatggc tcagaaattg cgggtggccc acccggttct atcgatgaag atggcgatcg
     5881 ttatgtggag atctggaatc tggtctttat gcaatataac cgctttgaag acggaacctt
     5941 aagtcctcta cccaaacctt cagtggatac aggtatgggt ttagagcgca ttgcagcggt
     6001 cgttcaaggt gtgcacagta attatgatat cgatattttt aaaaaattaa tccaatccat
     6061 tattgaaatc ggcaatatac aagatacgaa aaatcaatcc ttacgggtga tagccgatca
     6121 tatacgttcg tgtgcatttt taattgttga tggcgtatta ccttcaaatg aagggcgtgg
     6181 ttatgtttta aggcgtattg cacgtcgtgc gatacgtcat ggccatcgtt taggatttac
     6241 ggatcctttt ttctatcgat tggttaagcc cttggcgcag gtgatgggcg aagcttatcc
     6301 agaattgata gaaaaaattt cgttgataga acgtgcatta cagcaagaag aagtacaatt
     6361 taatcgtacc ttggatcaag gtttaaaaat tttagagaac gatttgacga atatcaccgg
     6421 aaatttaatt ccgggtgaga cggtctttcg tttgtacgat acttatggtt ttccggtaga
     6481 tttaaccaat gatattgcaa gagagcgtgg cttaactata gacatggcag gatttaatga
     6541 atccatggaa gcacaaaaaa cacgtgcgcg cgagtcaagt caatttggag ttaattataa
     6601 tgatcatacg attcccacgc ataatcacac aactgatttt gtaggttatg attctctaaa
     6661 agcagaatgt ttaattgtgg gtttatatca agatggcaag gccgtgtccg aactgcaaga
     6721 gggtgaggcg ggggctgttg tattgaaaca ctcacctttt tatgcggaat caggtgggca
     6781 actgggtgat caaggtgtat tcacttttaa agaagggatc tttgaagtcc agaatacccg
     6841 taaagtgggc gatgcacact ttcatcatgg catcgttaaa aagggtgttt tttcgattga
     6901 tgatcagatt ttagccagtg tggatcactt aaagcgaaat gcgacagcac ttaatcactc
     6961 ggcaacgcat ttattacatg ctgcacttcg gcagattata ggtacacatg tacaacaaaa
     7021 aggatctttg gtcgaacccg atcgattacg ttttgatttt tcacattttg aagccttaag
     7081 tcgcgagcaa atcaccgcaa tcgaaaggtt ggttaacgaa aaaattcgcg acaatactgc
     7141 tgttgaaaca gaagttttgc ctattgaaga ggcgatagcc aatggcgcta tggctttatt
     7201 tggtgagaag tatggcaagg cggtacgtgt tttacatatg ggcagtgatt tttcggtaga
     7261 gctttgtggg ggaacgcatg ttaagcatac aggcgatatc ggtgttttta aaataacggc
     7321 tgaatatggt attgcttctg gtattcgccg tttagaggca ttaaccggtg cacgcggatt
     7381 agattggatg aatgcgatag aggcacagcg cgatcgtcta gcgttatttt taaagtgcaa
     7441 acccgaaaca ctggaagaca agttacaaca attagcacaa aaaaatcgcc aattagaaaa
     7501 agatttgcaa acccttaagg cattaatgat gaccggcaaa gcgcatgatt tagaaaaagt
     7561 cttagaggaa aaaattcaaa agacacctca tggaatgcgc gtattggccg ctaaattgcc
     7621 tgtgggagac ataaaaacat tgcgtgaaac cgttgatcac tggaaaagta agctcggatc
     7681 cagagcagtg attgttttgg gcggtatcaa tgaccaaaat agggtatgtt tggtggcagg
     7741 tacaacgcgg gattgtcaag agcaatttaa agcacaggaa ttggtgaata ttgtggcagc
     7801 cgaggttggt ggaaaaggtg gtggtcgatc agatatggca gaggcaggcg gcactcagcc
     7861 agaacattta gataaggcat taggcttggt ttatgactgg gtttctaaaa cagaaagttt
     7921 atcgactaat attaatcacc cgatggcttg aggagaaaag gttcgtgtgt aataataaac
     7981 ccattttagg ctaagacaat ggccttgata gtgcagaaat ttggtggaac ttcggtaggc
     8041 agcattgagt gcattcgcca ggttgccgaa aaagtgatta aaacgcgcga ggcggggcac
     8101 gatctcgtgg tcgtagtttc tgcaatggcc ggtgaaaccg atcgtttagt gcgtttagca
     8161 caagaattga gtcaattgcc gaatccacga gaatttgacg ttttattgtc taccggtgag
     8221 caagtcgtaa tcgcattatt aagccttgct ttattggagc ggggttgccc tgcacgttct
     8281 tacacgggga ctcaggttca tattcgcacg gatagcgcac ataacaaggc gcgtatttta
     8341 ggtatagatg gctctaagat cttatccgat cttaaagcgg gaagagtggt tgttgtggca
     8401 ggttttcaag gaacggatga aatgggaaat attacaacgc tcggcagagg gggatccgat
     8461 atcacagcag ttgcattagc tgcggcgctt gatgcggacg aatgtcaaat ttatacagat
     8521 gtcgatggcg tttatacagc tgatcctaaa attgttgctg atgcgggtcg gatgtcgcaa
     8581 gtaaccgttg aagaaatgtt agaaatggcg agtaatggcg caaaggtaat gcagatccgc
     8641 gccgtagaat ttgcaggcaa atatcatgtg cctgtgcgtg ttctgtcgac ttttcaagag
     8701 ggaacaggca ccctaattat tgcagatgaa gccaatatgc agcattcggc agtctcgggt
     8761 attgcgttta atcaagatga agcaaaattg gttatttatg gtgttcccaa taaaccgggc
     8821 attgctgcac atattttagg ttcgatgagt gacgtgcata ttgaagtcga tatgattgtg
     8881 caaacgcaaa cagaagaaga aaaaacagac ttagcgttta ccgtgcataa acgtgatttt
     8941 caacaagcat tattgatttt agaaaatgtg gcaaaagcga taaatgcaaa acacattacc
     9001 agtgacgcgc acgtcgcaaa actttcatta gtgggcgttg gtatgcgaac gcacgcaggt
     9061 gttgcaagta aaatgtttca agtgttggga aaagagggaa ttaatattca actcatttcc
     9121 acatcggaaa ttaaaatttc ggttattatt aatgagcagt atttagagcg cggtgttcgt
     9181 gcattacatg atgcttttca attaggaaca gaagaaagaa agacaaagga gattgaacaa
     9241 tgttaatttt aacaaggcga gttggcgaga ctttaatcgt aggggatgaa gtgacggtta
     9301 cagttttggg ggtaaaagga aatcaagtgc gtattggtgt gaatgcacct aagaacgttt
     9361 ccgtgcatcg cgaagaaatt tatcagcgta tccaacgtga aaaacaagac ggcattgtgc
     9421 atgaaaaagc agatcagggt tcgaatgacg tagagcaaga cgaatctgat cattaaagtt
     9481 taccgtttta ttcataaaag accttttcta agattatctt ggctttttct taaagttaag
     9541 gtacattttt gggcattttc gtggtagact ttctagcttc tggggattag acccagagac
     9601 tagaaaggag agatggccga gtggctgaag gcgctcccct gctaagggag tatgggggtt
     9661 aacctcatcg agggttcgaa tccctctctc tccgccagcc ttcgctgaag ctacggctgg
     9721 caggctcgcc tgacaaaagg gggcaagtct ggacacagac acaaattctt ccttaacttt
     9781 ttccgtcagc gatagtattc cggaatttcc ggataactga atcttttgct aattctgcac
     9841 ttttgaaaag ggcagacaca gcggtctgcc cctacagtaa ttggtattca tgaattttac
     9901 attttaaaaa tattcctaa
//
LOCUS       DAORVT010000005         9346 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_3991_length_9346_cov_4.491120, whole genome shotgun sequence.
ACCESSION   DAORVT010000005 DAORVT010000000
VERSION     DAORVT010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9346
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3991_length_9346_cov_4.491120"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..1425
                     /locus_tag="PK389_00200"
     CDS             208..1425
                     /locus_tag="PK389_00200"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011627785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HQY22164.1"
                     /translation="MQIYLVGGAVRDKLLGLPQRERDWVIVGGTPEALLAEGYKPVGK
                     DFPVFLHPQTQEEYALARTERKTGKGYYGFECYASPDVTLEEDLSRRDLTINAMATTE
                     RGEIIDPFQGQADLKNKTLRHVSSAFAEDPVRILRVARFAARFAPLGFTVAPETINLM
                     RSMVASNEVDELVAERVWQEMHRALGEADPTQFFLVLRACGALQRIWPALDKLWGIPQ
                     PEQHHPEIDTGIHVMMVLGISAKLSQERLTRFAALCHDLGKGESPPEMWPSHRGHEER
                     GVPLIQAFCKRYRVPKEYGDLAKLVSRFHLHCHRVFELKPTTLLKTLERLDAFRQPER
                     FEKFLLACEADARGRLGRETTSYPQADRMREALQVAKAVLIKPLIDAGLEGAALGVRL
                     HQQRARAIEKAFD"
     gene            complement(1467..2615)
                     /locus_tag="PK389_00205"
     CDS             complement(1467..2615)
                     /locus_tag="PK389_00205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002691101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HQY22165.1"
                     /translation="MPIPSPEAFQHSMQLLAHIFSEIKHHGGKIPFAKYMDLALYTPG
                     LGYYSGGMQKFGMHGDFVTAPELSALFSKCLARQCQEILENLGDGDILEIGAGSGQMA
                     ADILLALTKANALPNYYYILELSADLKKRQQEKIATLCPRFFNRVVWLETLPLFDFKG
                     VILANEVLDAMPVHQFHIHQNILHEVEVTVQDDKLQHQISPTNNPHLLAHYHSQTWPT
                     EYTSEINLHLKPWIMSLSDILKSGVILIIDYGFPQHEYYHPQRSMGTLMCHYRHYAHH
                     DPFLYPGLQDITAHVDFTAIAEAASEIALDVLGFTSQAAFLFDTGLMTLVEPYNTTTD
                     KEIIVRNHAIQTLTSPAEMGELFKVIALGRNFEENLMGFNRMDRRYTL"
     gene            2738..3325
                     /gene="plsY"
                     /locus_tag="PK389_00210"
     CDS             2738..3325
                     /gene="plsY"
                     /locus_tag="PK389_00210"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017047525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HQY22166.1"
                     /translation="MLTLIVFCLLAYAIGSLSSAVIVCRLAGLPDPRTAGSKNPGTTN
                     VLRLGNKKAAIATLLGDVLKGFIPVFIVMHINPGAQFVGLVMLSAFLGHVYPIFFAFK
                     GGKGVATAVGTLLALSIPLGLLSVGTWGIIVYCFRISSLAALSAALLAPIYTFWLLNI
                     QYAIPVFIMSAFLLWRHRANIQRLLQGTEPKIGSS"
     gene            complement(3366..4151)
                     /locus_tag="PK389_00215"
     CDS             complement(3366..4151)
                     /locus_tag="PK389_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HQY22167.1"
                     /translation="MQTIARLLFGFLLYLAATPALANNKILICTDTNFWYPFTYLEDN
                     KPTGLHIDIIKTALRNLGYEAQFKATSWKQCLSDAEHGLVDAVATASYRDDRAAYLNY
                     PEGAAVDKKSPWRVSQVGYVLVTSAFDKQGKKNTYHFGGNFKTVPEPVRAPIHYSVVK
                     DLEKEGLEVREGKNSLYNFKQLAEEHTGSVVDIEDVSLYFATQPQFADKLIIQKKRLN
                     YKSYFLAFSKSGSIKRDASEIIWKKIAKIRDNPKQIAEFLKHY"
     gene            4314..4556
                     /locus_tag="PK389_00220"
     CDS             4314..4556
                     /locus_tag="PK389_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HQY22168.1"
                     /translation="MSPLTVSEARTQLYNLVDEVSLHHEPVLITGKRHNAILVSEEDW
                     SSIQETLFLMSIPNMRESIKRGLQTPPDACDRDLDW"
     gene            4553..4816
                     /locus_tag="PK389_00225"
     CDS             4553..4816
                     /locus_tag="PK389_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006632711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HQY22169.1"
                     /translation="MSWALVYTKDAQKDAKKLTLRGLRKKAEVLLEILKVDPFQNPPP
                     YQKLVGDLLSAYSRRINVQHRLVYQVLKTEGIVKIIRMWSHYE"
     gene            4843..5589
                     /locus_tag="PK389_00230"
     CDS             4843..5589
                     /locus_tag="PK389_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005373494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY22170.1"
                     /translation="MQKVIIVTGSSRGIGAATAIKAASLGYKVCVNYMRNDAAANAIV
                     DLIYKNGGQAIAVKADVSKELEVEKLFKVVDKELGVLTALVNNVGILEPKMRVEEINL
                     ARLERIFSTNIASYFLCAREAVRRMSTIHGGKGGAIVNVSSAASRLGSAGEYVDYAAS
                     KGAIDTLTIGLSVEVANENIRVNGVRPGFILTDIHASGGEPDRVNRIKTQLPMQRGGE
                     PAEVANAILWLLSEEASYVTGSFIDLAGGK"
     gene            complement(5653..6681)
                     /gene="tsaD"
                     /locus_tag="PK389_00235"
     CDS             complement(5653..6681)
                     /gene="tsaD"
                     /locus_tag="PK389_00235"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017005144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HQY22171.1"
                     /translation="MSIRRVLGIETSCDDTGVAIFDAEAGLLAHALHSQTATHIPYGG
                     VVPELASRDHACKLLPLLEKVFQAADVPKQSIDGIAYTAGPGLIGALLVGAAFGKALG
                     YAWGIPTVGVHHMEGHLLAPMLENPAPAFPFVALLVSGGHTMLVEVMGIGQYKILGES
                     LDDAAGEAFDKVAKHLGLPYPGGPALAALAEQGIHNRFVFPRPLTQKPGLDFSFSGLK
                     TFTMNTLRATDQTTQDKADIAWGFQDAVVDTLYLKCKRALQFTGLNRLIVAGGVGANR
                     ELRTKLQTLEQTHNVTVFYPRLEFCTDNGAMIAYAGYQRLKMGGDHNLEILASPRWNM
                     ESLSTPKL"
     gene            6778..7008
                     /gene="rpsU"
                     /locus_tag="PK389_00240"
     CDS             6778..7008
                     /gene="rpsU"
                     /locus_tag="PK389_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HQY22172.1"
                     /translation="MPSVRMRDGESIDSAIRRFKRAVEKAGIPKELRRREFYQKGSMI
                     RKRKAAAARKRHLKKLTREQMNGQPGHREARA"
     gene            7030..7485
                     /locus_tag="PK389_00245"
     CDS             7030..7485
                     /locus_tag="PK389_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009669839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HQY22173.1"
                     /translation="MTEESLKNKIQASMIATMRAQDKPKLAVIRLIQAAIKQQEVDER
                     ITLDDEQILSTLDKMIRQRRDSIKQFEAASRHDLAEKEIFEIGIIQEYLPTPLSHEEI
                     QTFIQQAIQEVGAASIRDMAKVMNLLKPKLQGRADIGEVGSLIKNQLAS"
     gene            7491..9251
                     /gene="dnaG"
                     /locus_tag="PK389_00250"
     CDS             7491..9251
                     /gene="dnaG"
                     /locus_tag="PK389_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HQY22174.1"
                     /translation="MTGQIPQSFIEELLARTDIAEIIGGRISLRRAGANFIACCPFHQ
                     EKTASFTVSAAKQFYHCFGCGVSGDAIQFLTEHDGLHFVEAVETLASRAGLAMPIETQ
                     ESNQTARSAPVYEILAIAAAFYQTQLRTHAVAEKAHQYLKKRGLTGQIAKQFGLGFAP
                     PGWDALVKNLGTDQTRIDALILAGLVVKKEAGRYYDRFRDRILFPIRDRRGRVVGFGG
                     RVMGSEGEPKYLNSPETLVFNKGSELYGLYEARLGNRALTSLLVVEGYMDVVSLAQFG
                     IKNVVATLGTAVTEKQIDALFRQASQLIFCFDGDKAGQAAAKRALPLILPHMKEGRRV
                     RFMLLPAGEDPDSLVRKLGQVAFLDRVERASLLSDFLFEDLTAHLDLHHLDSRAQLVT
                     LAKPLIKLVPPGVLQQMLYDRLAELAGVNSQVVQKKYGNKKNNILSNRAVRELALPPS
                     PAYQLVAMMLEHRELVLKAGESTEFRGLEIAGANLLCATIEILRFDSTIGIEAFKEQL
                     PENCVSQFSYDALKRIVDRIPRGGVDQEFLGALQNLRGAAQEQAMESLLIKAKTGVLS
                     TDEKAQLKNLLAQREKNRVD"
BASE COUNT         2605 a         1774 c         2213 g         2754 t
ORIGIN      
        1 cagtcattcc gacggttatt tgcgtgatct attttggcgc ttcacccatt atgacttgga
       61 tattatgggc gacgtatgga tttttaaatg cggttttttt ctttgtgcga tataaaaaca
      121 aatgctggaa tgcagtgcaa tccttgcatg ccttatctta ataaggtact gtgcaaaaat
      181 aatttgtttt tttaagagag gtgttgtttg caaatttatt tagtgggtgg tgcagttcgt
      241 gataaattac tcggcttgcc gcaacgcgag cgagattggg ttatcgttgg tggaacaccc
      301 gaagcgttat tagcagaggg ttataagccc gttggcaaag attttcctgt ttttttacac
      361 cctcaaaccc aagaagaata tgcattagca cgcaccgagc gtaaaacggg taagggctat
      421 tatgggtttg aatgttatgc cagtccggat gttacattag aagaagattt atcgcgtcga
      481 gatttaacaa ttaacgcgat ggccacaacc gaacgaggcg aaattatcga tccctttcag
      541 ggacaagcag atctcaaaaa taaaactcta cggcatgtat cgtctgcttt tgctgaagat
      601 ccggtgcgta ttttgcgtgt tgctcgtttt gctgccagat ttgcgccatt agggtttacc
      661 gtagcaccag aaacgatcaa tttaatgcgt tcaatggttg cttcaaatga ggtcgacgaa
      721 ttagtcgcag agcgtgtttg gcaagaaatg catcgtgctt taggagaagc tgatcccact
      781 caattctttt tagtcttgcg tgcatgtgga gcattacaac gaatttggcc tgcgctcgat
      841 aaattgtggg ggatcccaca accagaacaa catcatcccg aaatagacac gggtattcat
      901 gtcatgatgg tattaggtat ctcagccaag ctctctcagg agagattaac gcgctttgca
      961 gcgctgtgtc atgatttagg caagggcgaa agcccgcccg aaatgtggcc cagtcaccgt
     1021 ggacatgaag aacggggtgt gccgttaatt caagcttttt gtaaacggta tcgtgtgcca
     1081 aaagaatatg gcgatttagc caaattggtt agtcgttttc atttacattg tcatcgcgta
     1141 tttgaattaa aacccaccac acttttaaaa actttagagc gtttagatgc gtttagacaa
     1201 cctgaacggt ttgaaaaatt cttgcttgct tgcgaagcgg atgcaagagg tcgtttggga
     1261 cgagaaacga catcgtatcc gcaagcagat agaatgcgag aggccttgca agtcgcaaaa
     1321 gcagttttga ttaagccctt aatagatgca gggttagaag gtgcagcatt aggtgttcgg
     1381 ttgcatcaac aacgcgcacg cgcgattgaa aaggcttttg actaaggctc tatgcaaaat
     1441 aatttttact tttatttgcg cagagtctaa agcgtatagc gtcgatccat tcgattaaat
     1501 cccatcaaat tttcttcaaa atttctgcct aatgcaatca ctttaaataa ttctcccatt
     1561 tctgcaggag aagtgagtgt ttgaatggcg tgattgcgaa caataatttc tttatcggtt
     1621 gtcgtattgt atggttctac caacgtcatc agcccggtgt cgaatagaaa ggcagcttgc
     1681 gaggtgaacc ccaaaacatc gagtgcaatt tcgctggcag cttcggcgat ggccgtaaag
     1741 tcgacatgtg ctgttatatc ttgtaatccc gggtataaaa aaggatcatg atgtgcataa
     1801 tgcctataat ggcacattaa agtgcccatc gatcgttggg ggtgataata ttcatgctga
     1861 ggaaagccgt aatcaataat taatataaca cctgatttta aaatgtctga tagactcatg
     1921 atccagggtt ttaagtgtaa gttaatttcg gacgtatatt ccgttggcca cgtttgagaa
     1981 tgataatgtg ctaaaagatg tggattgttt gtcggagata tttgatgctg gagtttgtca
     2041 tcctgtacag tgacttcaac ttcatgcaat atattttggt gtatgtgaaa ttgatgaaca
     2101 ggcatggcat ctaatacttc gttggcgagt ataacacctt taaaatcaaa caacggaagt
     2161 gtttccagcc agacgacacg gttaaaaaat ctggggcata gcgttgctat tttttcttgt
     2221 tggcgttttt ttaaatccgc acttaattct agaatataat aatagtttgg tagcgcattc
     2281 gcttttgtca atgccaaaag tatatcggca gccatttgtc cgctgcccgc accgatctct
     2341 aaaatatcgc catcgccgag attttctaaa atctcttgac attggcgcgc tagacatttt
     2401 gaaaagaggg cagagagttc cggcgccgtc acaaaatcgc cgtgcatgcc gaatttttgc
     2461 atgccaccac tgtaatatcc gagcccgggg gtatataacg caagatccat atatttcgca
     2521 aaggggattt ttccaccatg atgcttaatt tcagaaaaaa tatgcgctaa caactgcatg
     2581 ctatgttgga atgcttctgg gctgggtatg ggcatttgat taggtaataa agtattaatg
     2641 ggcacacccc gaaacgtaca cgttcctaaa aatcctatgt taaatacatt ttcatcatga
     2701 taccattttg aattctactt tcaagatgaa atatagcatg ttaactttga tagtattttg
     2761 tttattggct tacgctattg gatcattgtc gagcgctgtg attgtgtgtc gcttggcagg
     2821 cttgcctgat cctcgcactg ccggctctaa aaatccggga actacgaatg tactacgcct
     2881 tggtaacaaa aaagccgcta ttgcgacatt gcttggcgat gtcctgaaag ggttcattcc
     2941 tgtttttatt gtcatgcata ttaatccagg ggctcagttt gtaggattgg tgatgctctc
     3001 tgcattttta gggcatgtgt accccatttt ttttgcattt aaagggggca aaggtgttgc
     3061 gacagcagtt ggcacactgc tcgcgctttc tatccccctg gggcttctta gcgtggggac
     3121 atggggcatt attgtttatt gttttcgtat ttcttctttg gcagcattaa gcgcggcgct
     3181 gttagcaccc atttatactt tttggttgtt gaatatccaa tatgctattc ctgtttttat
     3241 catgagtgca tttttgttgt ggcgtcatcg tgccaatata caacggttat tacaaggtac
     3301 ggaacctaag attggttctt catagtgttt gatttttgta tccatcgctt ttcagattat
     3361 gggttctagt agtgctttaa aaattctgct atttgctttg gattatcgcg gatcttggca
     3421 atttttttcc agatgatttc tgacgcatcg cgttttatag aacccgattt tgaaaaagca
     3481 agaaagtagg atttataatt taaacgcttt ttttggatta ttaatttatc tgcaaactgg
     3541 ggttgtgttg caaaatatag cgagacgtct tcaatgtcaa cgacagatcc agtgtgttct
     3601 tctgctaatt gcttgaagtt atacaacgaa ttttttcctt cacgtacttc taacccctct
     3661 ttttcgagat ctttaacaac agagtagtga atgggggcgc gaacaggttc tggtactgtc
     3721 ttaaaattcc cgccgaagtg ataagtattt tttttgccct gtttgtcgaa tgcggatgtt
     3781 actaatacat aaccgacttg tgatacgcgc caaggggatt ttttatcaac ggcagcgcct
     3841 tctgggtagt taagataagc agcacgatca tcgcgataag aggcagttgc gacagcatca
     3901 acaagaccgt gttctgcatc acttaagcat tgtttccaag aagtggcttt aaattgagcc
     3961 tcatatccta aattacgcag tgctgtctta atgatatcaa tatggagacc ggtaggttta
     4021 ttgtcttcaa gataggtaaa aggataccaa aagttagtgt ccgtacaaat taatattttg
     4081 ttgtttgcaa gggctggcgt cgcagcgaga tataacaaaa atccgaataa aagtcgtgcg
     4141 atggtctgca tcgtattctc cttagagggc aattttcaaa gggtacattg atatagtgtg
     4201 attgatgttt agtttggcat taattcctga aggtgtccat atatacacct tgcataatcg
     4261 tacgaaattg agtacgcttg tgttgtgagg atcatttttg gaggaaaacg tgtatgtccc
     4321 ctttaaccgt cagcgaagcg cgtactcagc tttataatct tgttgatgag gtttctttgc
     4381 atcatgagcc tgttttgatt acaggaaagc gccataatgc gatattggtt tccgaggaag
     4441 actggtcttc aattcaggag actttgtttt taatgtctat tcccaatatg cgcgaatcta
     4501 taaagcgcgg gttacaaaca cctcccgatg catgtgatcg agatcttgat tggtgagttg
     4561 ggcgcttgtt tatactaaag acgcacagaa agacgcgaaa aagctcactt taagggggct
     4621 taggaaaaaa gctgaggttt tgctcgaaat tttgaaagtc gatccttttc agaatccacc
     4681 gccgtatcag aaattggtag gggatttact gagtgcttac tcgcgacgca ttaatgtgca
     4741 acatcgtctt gtctatcagg tattaaaaac cgaaggtatt gtaaaaatta ttcgcatgtg
     4801 gtcgcattat gaataaaccc ttcaattgga tctaaaagta tcatgcaaaa agtcattata
     4861 gttaccggga gtagccgagg aattggagct gctacagcga taaaagcagc atcattaggt
     4921 tataaagtct gcgtaaatta catgagaaat gatgcagcag caaatgcaat tgtagatctg
     4981 atctataaga atggtggaca agctatagct gtgaaagcgg atgttagcaa agaattagag
     5041 gtagaaaaac tatttaaagt tgttgataag gaactaggag tattaacggc cttagtaaat
     5101 aatgtcggta ttctcgaacc aaaaatgcga gttgaggaaa taaatttggc tcgtttagaa
     5161 agaattttca gtaccaatat tgcaagctat tttctttgtg cgcgcgaagc tgtgcgacgt
     5221 atgtcaacga tccatggggg aaagggaggc gctattgtta atgtctcttc tgctgcttcg
     5281 cgtctgggat cagcaggaga atatgttgat tatgctgcgt caaaaggtgc gattgatacc
     5341 ttaaccattg gtctttctgt tgaagtggca aacgagaata ttcgagtcaa tggcgtgaga
     5401 cctggtttta tccttacgga tatacatgcc agtgggggcg aacccgacag agtgaatcgc
     5461 attaaaacgc aacttccaat gcaacgtggt ggagaacctg cagaagttgc taatgcaatt
     5521 ctttggttgc tttctgaaga agcctcttac gtgacgggat cgttcattga tttggcaggg
     5581 ggtaagtaac tcaagatcgg atacaaaaag agaacaattg aatgatagac ccatgagtta
     5641 aattgacgga cgttacagct ttggtgtcga taacgactcc atgttccacc gtggagatgc
     5701 taaaatctca agattgtgat cgccgcccat ttttaatcgc tgatacccag cataggcaat
     5761 catcgcgcca ttgtcggtac aaaattctaa tcggggataa aagacggtta cattatgcgt
     5821 ttgttctaat gtttgtaatt tagttcggag ctctcgatta gcaccaacac ctcctgcaac
     5881 gataagtcga tttaatccgg tgaattgcaa tgcgcgtttg cattttaggt aaagtgtatc
     5941 gacaacagca tcttgaaatc cccaggcaat atccgcttta tcttgtgtgg tttgatcagt
     6001 cgcacgtaat gtgttcattg taaaagtttt gagtccacta aaactaaaat ctaaaccggg
     6061 tttttgggtt aagggtcttg ggaatacaaa acgattgtgt attccctgtt ctgctaaggc
     6121 ggcaagtgca ggtcctcccg gatacggtaa gcctaaatgt tttgcaactt tatcaaaagc
     6181 ttcacctgct gcatcatcca gcgattcacc taaaatttta tattggccga tgcccattac
     6241 ttctaccagc attgtgtggc cgcccgatac caacaaagcg acaaagggga aagcgggtgc
     6301 gggattttcc agcatgggtg cgagcaagtg gccctccata tgatgtacac ctacggtggg
     6361 aatgccccaa gcgtacccca gcgctttgcc aaaagcggca cccaccagca aagcgccaat
     6421 cagaccgggt cctgcggtat aggcaatgcc atcaatagac tgtttaggta cgtcggctgc
     6481 ctgaaatact ttttctaaga gcggcagcaa tttacaagca tgatcacgcg aggctaattc
     6541 gggcacaacg ccgccgtacg ggatatgtgt tgcggtttga ctatggagcg catgtgccaa
     6601 taggcccgcc tccgcgtcaa aaatagcaac gcctgtatca tcacaggatg tttcaatgcc
     6661 taatactctt cgtatgctca taaaaccttt ccaatcttct ccgactggtc tagaataaca
     6721 tgcttaactg tagatgggtt taattttaag taactaactt taggggtgtg atattcgatg
     6781 ccaagtgttc gtatgagaga tggggagtct attgattctg caatacgtcg ttttaaacgc
     6841 gcggttgaaa aggccgggat tcccaaagaa ttgcgtcgtc gtgaattcta tcaaaaaggt
     6901 tccatgattc gtaagcgaaa agccgctgct gcaagaaagc gtcatttaaa aaaattaacg
     6961 cgagagcaaa tgaacgggca gccgggtcac cgggaagcac gcgcttaagc gtgatggatg
     7021 gaggcaaata tgacagaaga atctttaaaa aataaaatac aggctagtat gatagcgact
     7081 atgcgggcgc aagataagcc aaagcttgct gtgattcgtt tgatccaagc ggcgattaag
     7141 cagcaagaag tggatgagcg cattactttg gatgatgagc agattttaag cacgctggat
     7201 aaaatgattc ggcaacgtcg tgactcaatt aaacaatttg aagcagcatc acgccatgat
     7261 ctcgccgaaa aagaaatttt tgagatcggc atcattcaag aatatttgcc aacgccctta
     7321 agtcatgaag aaattcaaac gtttattcag caagccattc aagaagtggg tgcagcatct
     7381 attcgtgata tggctaaagt gatgaatttg ttgaagccca agctgcaagg tcgtgcagac
     7441 ataggcgaag tcggtagttt aatcaaaaat caattagcgt cgtaagtgtt atgacggggc
     7501 aaattcccca atcatttatt gaagaactct tagcgcgtac tgatattgca gaaattattg
     7561 gtggacggat ttcgcttcgt cgcgccggtg cgaattttat tgcatgttgc ccctttcatc
     7621 aagaaaaaac cgcttccttt acggtgagtg ccgctaaaca attttatcat tgttttggtt
     7681 gcggtgtgag cggcgatgcc attcaatttc tcacagaaca cgatggacta cattttgtag
     7741 aagctgtcga aacattggca agtcgtgccg gtttagcaat gccgatagaa acacaagaaa
     7801 gcaatcaaac ggcgcgatct gcaccggttt acgaaatatt agcgatcgcc gctgcttttt
     7861 atcaaacgca attacgcaca catgcggtag cagaaaaagc gcatcaatat ttaaaaaaaa
     7921 gaggtctaac cggacaaatt gcaaaacaat ttggattagg atttgcgccg cctggatggg
     7981 atgcgttggt taaaaatttg ggcacagatc aaacgcgcat agacgcatta attttggcag
     8041 ggttagttgt taaaaaggaa gcgggtcgtt attacgatcg ttttcgcgat cgtattttat
     8101 ttcctattcg ggatagacgt ggtcgtgttg tcgggtttgg tggtagagtt atgggctctg
     8161 aaggtgagcc taagtatttg aattctcccg aaactctggt ttttaacaag ggttcagagc
     8221 tgtatggatt atatgaagca agattaggaa accgtgcctt aacgagtctt ttagtggttg
     8281 aaggttatat ggatgtggtc agcctcgcgc aatttggcat taagaatgtg gttgcaacat
     8341 taggaaccgc tgtcaccgaa aagcaaattg atgcgttatt tcgacaggcc tctcagctga
     8401 tattttgctt tgatggtgat aaggccggtc aagctgcggc aaaacgcgcc ttgcccctta
     8461 ttttgccgca tatgaaagaa gggcgtcgtg ttcgctttat gttattgcca gcaggtgaag
     8521 atccggattc gctggtacgg aaattgggac aggtggcttt tttagatcgt gtggaacgtg
     8581 caagtctttt gtcggatttt ttgtttgaag atttaaccgc gcatttagat ttgcatcatt
     8641 tagacagtcg tgcccaatta gtcaccttgg caaaaccact gattaaatta gtaccacccg
     8701 gggtattaca acaaatgctg tacgaccgtt tagcagagtt agcaggcgta aattctcagg
     8761 tagtacaaaa aaaatatggg aacaaaaaga acaatatctt gtctaataga gccgttagag
     8821 agctagctct tccgccgagt cctgcttatc agctggttgc catgatgtta gaacacagag
     8881 aactcgtttt aaaggcaggg gaatcgacgg aatttcgagg tttagaaatt gcaggtgcaa
     8941 atttattgtg cgccactatt gaaattctac gttttgattc tactattggc atagaagctt
     9001 ttaaggagca gcttccagaa aattgtgtta gtcagttttc ttatgatgcc ttaaagcgca
     9061 tcgtcgatcg cattccacgg ggtggggtgg atcaggaatt tttgggcgct ttgcagaatt
     9121 tgcgcggagc agctcaagag caggccatgg aatctctact gataaaggcc aaaacgggcg
     9181 tgctttcaac ggatgagaaa gcacaattaa aaaatttgtt ggctcaaagg gaaaaaaacc
     9241 gagtggatta accgatttat agtgataaaa ttggaatttc gcgggtgggt ttagcttttg
     9301 gccaaataaa aatgagcgta gagaataaat tatgagtact tatgta
//
LOCUS       DAORVT010000006         9040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_4227_length_9040_cov_3.688703, whole genome shotgun sequence.
ACCESSION   DAORVT010000006 DAORVT010000000
VERSION     DAORVT010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9040
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4227_length_9040_cov_3.688703"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1716)
                     /gene="pepN"
                     /locus_tag="PK389_00255"
     CDS             complement(<1..1716)
                     /gene="pepN"
                     /locus_tag="PK389_00255"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HQY22175.1"
                     /translation="MNIEIEAKPKTIYLKDYQPSLYQIASIDLNFDLYNDKTLVHSIL
                     VCQLNKTHPTYQEKKPRTLILNGEKLVLHAIQLDGRTLSSSEYQVDQKSLTLFNVPEH
                     FRLEIKTEIYPDKNTELAGLFRPKTLFCTQCESEGFRRMTYYLDRPDVMSKFTTTISA
                     DKRQYPVLLSNGNCIARGNADNGRHWVKWEDPFLKPCYLFALVAGDLVSIEDYFVTKT
                     GRLVALKIFVERENLDKCQYAMEALKKSMRWDEDTYGREYDLDIYMIVAVNDFNMGAM
                     ENKGLNVFNSKYILARPETATDADYQNIDAVVGHEYFHNWSGNRVTCRDWFQLSLKEG
                     LTVFREHHFSKDISESTVSLIENVRILRSHQFAEDSGPMAHSVRPESYMEINNFYTMT
                     VYEKGAEVIRMLKTLLGWETFRSAMDLYFERHDGQAVTTDDFVAAMEAASQQDLSQFK
                     LWYQQAGTPEITVQERYEEKTKTYYLTLKQFCPLTPEQPTKQPMVIPVSVGLLDKVGN
                     NLLSQSEILILKEKEQTFSFPNIQHPPILSVLREFSAPVKVHRDITDEQLAFLPMLFS
                     ADALQP"
     gene            complement(1822..2796)
                     /locus_tag="PK389_00260"
     CDS             complement(1822..2796)
                     /locus_tag="PK389_00260"
                     /inference="COORDINATES: protein motif:HMM:NF025699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HQY22176.1"
                     /translation="MREVNVKWPWEKGNILVISIISLVVLAGLSVAVLSTSVSEKKLA
                     GNEQEQNLAKQAAEYAVTMGKKDLSEKWAVGMCECQTSPCSCSTNAPWVWLTAQFVNA
                     DPVDSSETWWNNFGTAVAKPTAQLFSAPQYIIIDLGCDVVANVMRFRIIGRGLGTKQE
                     SVAFSDVTFSMPFNKQNVSSGQTSPVDALVDGVVQTTFNMAQFTASASFYKKGVQCQT
                     GASSAQCEMNCAGQVRVMAWTADSHCAPTYGSWAGVGVPSTVNLTLAGAPHTITCQMQ
                     VPPPPPPPPGGGTENPHDNGKHKGYAYGHTKQDNWENGNSDNPGSTPP"
     gene            complement(2837..4240)
                     /locus_tag="PK389_00265"
     CDS             complement(2837..4240)
                     /locus_tag="PK389_00265"
                     /inference="COORDINATES: protein motif:HMM:NF027400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HQY22177.1"
                     /translation="MSRLLTKNKQYYQIKGVSLISLVIGVAVSAFLLLIISQLAMIAK
                     RNYKQSQNIIGLSNNAREAMAFLTNNVQISGFGIMQPVAADSLQLNSTSYAGAPPTGG
                     PDQLNHFVYIGYYQENNSPNRLFPNQVIANGATPESCATLVSVNLPTVQFFGLQSCGQ
                     CWTPNPAGGAFDKTLLVTNPADTTATCCEPVGASCTPPTTANCGIYNCGGSLQNAVYQ
                     KMMQTVCVGTNCAGADPASVPNGDSLTVYFSNPGPGVFSTFTESDVPAATTQPPGSLS
                     SYTFYADSVTATLRAKDSFTNLTYNMANNVEYMAVLVGESDLYSSVNLGTLYSVPNMS
                     RFVTYNTANLYPYRITAIRVALVVRSQDQILPVVPANTTLQVLRGNNGNWITYKAPSD
                     RYLRKVFTKTIYLQGYGLPAYRTHCTQVGEFWYMKTGGIPYAPTWTVNDQCCGGSPCK
                     AYTQNTCEAQRMTGACW"
     gene            complement(4237..4776)
                     /gene="pilV"
                     /locus_tag="PK389_00270"
     CDS             complement(4237..4776)
                     /gene="pilV"
                     /locus_tag="PK389_00270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HQY22178.1"
                     /translation="MKKYSSGYGLLEVLVSLAVISIGALGFARAQVSALQITTDAQLR
                     TSATILLDDMVGRIQANAGEAWQGIKSGYQTGTPALNVNCLSTNGSICTGNQMAMHDL
                     ADWNALIASVFPATSNAVGIVCLDATPGNPAQACSPPAINPNPVIFTIKIFWKSWKNR
                     GGATFDQSVVTIVEPPLQR"
     gene            complement(4924..5718)
                     /locus_tag="PK389_00275"
     CDS             complement(4924..5718)
                     /locus_tag="PK389_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010391775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2797 domain-containing protein"
                     /protein_id="HQY22179.1"
                     /translation="MRKLKSTLSDPVQYYLPIGEVELPLNGCLGKIVTLKFLGDIQCV
                     HCQRKIKKSYSQGYCFPCAQTLARCDFCIVKPEKCHFHLGTCREPEWGKIHCFIPHIV
                     YLANTSGLKVGITRETQIPTRWIDQGAVQALPIFRVKNRYHSGLVEVVMKSMVADKTD
                     WRKMLKGQAENLALFEKRDELLKEIEWPNDLRDENAIEILQNQNVVSIQYPILSYPEK
                     VTAFNIEKTREISGTLLGIKGQYLIFDKGVLNIRNLTGYNISFFSD"
     gene            complement(5735..6208)
                     /locus_tag="PK389_00280"
     CDS             complement(5735..6208)
                     /locus_tag="PK389_00280"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HQY22180.1"
                     /translation="MSTQPRLRVTARALIIEQKKLLFVSDEGTYWYLPGGKIENSETL
                     TACVEREVYEETGLVVKAGLLRHVLECFDIENHVHNIHLYFETVVLEGAISDNWNDEF
                     GSVQFCKYFSLAEIQKNSTILPRFLAEGDWCHPSPKFHKIYEGVVKMRGFEMVEL"
     gene            complement(6205..7491)
                     /locus_tag="PK389_00285"
     CDS             complement(6205..7491)
                     /locus_tag="PK389_00285"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY22181.1"
                     /translation="MNKTKDRTKAVTGSAVTMCVLAILFYYYEYYLRVAPSVMSSELK
                     LTFNLSEAAFGNLAAFFYYAYTPMQIPVGMMMDRFGPRRLLTFACFLCVLGTVLFGIT
                     GQFWIAAMGRFLLGFGSAFAYVGVLKLSNVWLPKKYFAFMAGLCTTLGMFGGISCAVL
                     MSHLMLRMGWQSTLYYSAAAGIVLMLILWLVLRDGTRHEKQDPSEMDLAHEVHLKLTG
                     LKQIICSSQMWITGLIGLLTFLPITGFAEIWAMSFLETVGLSKFDAATGASMLFLGFA
                     VGAPLWGIISDAMQSRRIPLIIGSFLAASFMALVVFMPTTSLIWMYSLLFLSSFFASV
                     EILIFAVSNDISRHSVSATAIAFTNMVVMVGGVLLPPLIGKILDDAILVVNNSPVLTV
                     YDFSIALAVLPLGLVLSGILSLFLRESYHKHPKVIK"
     gene            complement(7531..7607)
                     /locus_tag="PK389_00290"
     tRNA            complement(7531..7607)
                     /locus_tag="PK389_00290"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7571..7573),aa:Pro,seq:ggg)
     gene            7778..>9040
                     /locus_tag="PK389_00295"
     CDS             7778..>9040
                     /locus_tag="PK389_00295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP bacteriocin system secretion protein"
                     /protein_id="HQY22182.1"
                     /translation="MQEELETTKASKLKFRQEALLELHAPDQIDKLIEVVSPKAWIML
                     LSFYMLLISLIVWVIFGSIPTRVEGKGILLVENGSVYNAVAPPGGGQIADILVQQEQE
                     IKKGDIIARLERPDLTEKLQLAKNYVEQLKREQEELSQTSKKELAIRHEKISQQISML
                     DSSLASGAKNLKDIETLLNLKEENFKKGILIWQDIENTRRDYYSAKDRLEQLQNQITQ
                     LKTQEDDFKDQWRQRLKDKELTILAEQLKMNNMAAEVTVSKTVVSPADGIIISVNTSV
                     GKMVPDGGSVVTITSLGEGLDALIFMLPHEGQRVTPEMPALITPTTIEKEEYGSMKGK
                     VLSVSEFPQAAETIIALLHNQEMAKQFVESGAPTGIRIRITRDPNTFSGYQWSSSKGP
                     NKKISPGTLAEVRITVREQPPISLVIPAF"
BASE COUNT         2843 a         2034 c         1628 g         2535 t
ORIGIN      
        1 gggttggagc gcatcggcag aaaacagcat aggcaaaaaa gcaagctgtt catctgtaat
       61 gtctctgtgc acttttacag gcgctgaaaa ttctcttagt acagataata tgggcggatg
      121 ctgaatatta ggaaaactaa aagtttgttc tttttctttt aagattaata tctctgattg
      181 tgagagaaga ttattaccca ctttgtctaa aagcccaacc gaaacgggga taaccatcgg
      241 ctgcttagta ggttgctctg gcgttagcgg gcaaaactgt ttcagtgtta aataatacgt
      301 ttttgttttt tcttcatagc gttcttgcac agtaatctcg ggggtgcctg cctgttgata
      361 ccacaattta aattgagaga gatcttgctg agaagcggct tccatggcag caacaaaatc
      421 atctgttgta acagcctgcc catcgtgccg ttcaaagtat aaatccatag cacttctaaa
      481 agtttcccat cccaagagcg tttttagcat acgaataact tccgcacctt tttcatacac
      541 agtcatcgta taaaaattat taatttccat ataggattct ggtcgcaccg aatgcgccat
      601 tggaccagaa tcttccgcaa attggtgtga acgtaagatg cgaacatttt caatcaagct
      661 aacggttgat tctgaaatat cttttgaaaa atggtgttct ctaaaaaccg ttagcccttc
      721 ttttaaactt aattgaaacc aatctcgaca agtcacgcga tttccagacc aattatgaaa
      781 atattcatgc ccaaccactg catcaatatt ttgataatca gcatctgttg cagtttcggg
      841 acgtgccaaa atatactttg aattaaaaac atttaagcct ttattttcca tcgcacccat
      901 attaaagtca ttgacagcga caatcatata aatatcgaga tcgtactcgc gaccatacgt
      961 atcttcgtcc cagcgcatcg attttttcag cgcttccatc gcatattggc acttgtctaa
     1021 attttctcgt tctacaaaaa ttttaagcgc aacgagacga cctgttttag tcacaaaata
     1081 gtcttctata gaaactaaat cgcccgcaac aagtgcaaat aaataacaag gcttcagaaa
     1141 gggatcttcc catttaaccc aatgacgtcc attgtctgca ttgcctctcg caatacaatt
     1201 tccattagat aataatacgg gatactgacg tttatctgca gaaatcgttg tggtaaattt
     1261 ggacataaca tctggtctgt ctaaataata tgtcatgcgc cgaaaacctt cggattcaca
     1321 ctgggtacaa aataaggttt tgggacgaaa tagccccgct aattctgtgt ttttatctgg
     1381 atagatctct gttttaatct ctaagcgaaa atgttcaggc acattaaaaa gagttaaaga
     1441 tttttgatca acctgatatt cactcgagga tagcgtacga ccatccagtt gtatcgcgtg
     1501 caatacgagt ttttcgccat tcaaaatcaa tgtgcgcggt tttttttctt gatacgtagg
     1561 atgtgtttta tttaattgac acactaaaat cgaatgcact agcgttttat cattgtataa
     1621 atcaaaattt aaatcaatag aagcgatctg atataaagac ggttgataat ctttgagata
     1681 aattgttttt ggttttgctt caatttcaat attcatactg tacctcaata aaaaccgacg
     1741 ttccggtgct agtatcgccc attaggttta acagagcacc tacaaacgtc ggctaaaaat
     1801 aacccttaag cggcatcaaa tttaaggagg ggtactgcct gggttatcac tattaccatt
     1861 ttcccaatta tcttgcttcg tatggccata cgcataaccc ttatgtttac cgttatcatg
     1921 cggattttca gtaccaccgc ctggtggcgg aggaggaggt ggtggcactt gcatttgaca
     1981 agtaatcgtg tggggtgcac ctgctaaagt tagattaaca gtactcggca cgccaacacc
     2041 tgcccacgag ccatatgttg gtgcacaatg gctatccgcc gtccatgcca tcacccgcac
     2101 ctggcccgca caattcattt cgcattgtgc agaagaagca cccgtctgac actgcacacc
     2161 ttttttataa aaagacgcac ttgcagtgaa ttgcgccata ttaaaagttg tttgtacaac
     2221 accatcgact aaagcatcta ccggtgatgt ttgaccagaa ctgacatttt gtttattaaa
     2281 aggcatcgaa aaagtgacat ccgaaaacgc tacggattcc tgcttcgtcc caagccctct
     2341 accaataata cgaaatctca tcacattagc aacaacatca caccctaaat caataataat
     2401 atattgtggt gcgctaaata attgagccgt tggtttagca actgctgtac caaaattatt
     2461 ccaccaagtt tctgatgaat caaccggatc cgcattaacg aattgtgctg ttaaccatac
     2521 ccaaggcgca tttgtcgaac acgaacaagg tgacgtctgg cattcgcaca taccaaccgc
     2581 ccatttttcc gataaatcct ttttacccat agtaaccgcg tattctgccg cttgttttgc
     2641 taaattctgt tcttgttcat taccagcaag ctttttttca ctcacacttg ttgatagaac
     2701 agcaacgcta agcccagcta aaaccaccaa cgatatgatg cttatcacca agatattgcc
     2761 tttttcccac ggccatttaa cattaacttc acgcattgtt ctcactccct attttatatc
     2821 taatcatccc tgttccctac caacatgcac ctgtcatcct ttgtgcttca catgtattct
     2881 gagtataggc tttacaaggc gacccgccac aacattgatc gtttactgtc cacgtgggtg
     2941 cataaggaat acctcccgtt ttcatatacc aaaattctcc cacttgcgta caatgcgttc
     3001 tatacgcagg caatccatac ccctgcaaat aaatcgtttt agtaaatact tttcgtaaat
     3061 atcgatcgct cggcgcctta taagttatcc aatttccatt attgccgcgt aaaacttgca
     3121 gcgttgtatt ggctggtact acaggcaata tttgatcttg agaccgaact accagggcaa
     3181 cgcgaatagc tgtaatacga tacgggtata aatttgccgt attataagta acaaaacgac
     3241 tcatgttggg tacagaataa agcgttccta aattaacaga agaataaaga tcactttccc
     3301 ccaccaacac cgccatatac tcaacattat tcgccatgtt atacgttaaa tttgtaaatg
     3361 aatcttttgc tcttaacgta gctgtgactg aatccgcata aaaagtatac gaagacaatg
     3421 agccaggcgg ttgtgttgtt gctgcaggca catcgctctc tgtaaaagta gaaaaaactc
     3481 ccggacctgg attcgaaaaa taaacggtta aagaatcgcc attcggaacc gatgccggat
     3541 cagcacccgc gcaatttgta ccaacacaaa ctgtttgcat catcttttga taaacggcat
     3601 tttgaagact gccaccacaa ttataaatac cacagttagc ggtcgtcggc ggtgtacaac
     3661 ttgcgcccac tggctcacaa cacgttgcgg tggtatctgc ggggtttgtg actaaaagcg
     3721 ttttatcaaa agcgccacct gcaggatttg gtgtccaaca ttgaccgcaa gactgcaatc
     3781 caaaaaattg tacagtcggt aggtttacac tcaccaaggt tgcacagctc tctggcgttg
     3841 cgccattcgc gatcacttga ttcggaaata atcgattagg gctgttattt tcctgataat
     3901 aaccaatata aacaaaatga tttaactgat caggcccacc tgtcggcggt gccccagcat
     3961 atgaagtgct atttaattgc aaagaatctg cagcaaccgg ttgcataatg ccaaagccac
     4021 ttatttgaac attatttgtt aaaaatgcca tcgcttctcg tgcattgttg cttaagccaa
     4081 tgatattctg actttgcttg tagtttcgtt ttgcaatcat ggccaactga gaaatgatca
     4141 ataataaaaa agcgctaact gctactccaa tcacaagaga gatcaaagaa acacctttga
     4201 tctgataata ctgtttattt ttagtcaata atctactcat ctttgtaagg ggggttctac
     4261 aatagtgaca acactttgat caaaagtcgc accccctcta tttttccagc ttttccaaaa
     4321 tattttaata gtaaaaatca caggatttgg attaatagca ggtggcgagc aagcctgtgc
     4381 cggattgcct ggcgttgcat ccaagcacac aataccaaca gcattacttg ttgcgggaaa
     4441 aacacttgca attaacgcgt tccaatctgc taaatcgtgc atcgccattt gattaccagt
     4501 acagatacta ccattagtac tcaaacagtt gacattcaaa gcgggtgttc cggtctgata
     4561 gccactcttt atcccttgcc aagcctcacc cgcattggcc tgaattcgtc cgaccatgtc
     4621 atccaataaa attgtcgcgc ttgttcttaa ttgagcatct gtcgtaattt gtaaggcaga
     4681 aacctgagct ctggcaaacc caagagcacc tatggatatc acagccagcg agaccaaaac
     4741 ctccaataag ccgtatccac tcgaatattt tttcatttct catccgtttc catgacttaa
     4801 tctacaacag aagatcctat ataagattat agtaaaagaa atgagaattg atccttggct
     4861 aatttgacct aaacctgaat gctggactta agaatggcaa tacattgaaa ttaattcaaa
     4921 agcttagtca gaaaaaaagc taatattata acccgtcaga tttcggatat tcagaacccc
     4981 tttgtcgaag attaaatatt gacctttaat acccaacaat gttcctgaaa tttctcgggt
     5041 tttttcaata ttgaaagcag tcactttttc agggtaagat aaaataggat attgaatcga
     5101 aacaacattt tgattttgta aaatttcaat agcattttca tctctgagat catttggcca
     5161 ttcaatctct tttaataatt catctcgttt ttcaaaaagt gctaagtttt cagcttgtcc
     5221 ctttagcatt ttacgccaat ctgttttatc cgccaccata cttttcataa caacttcaac
     5281 aagccctgaa tgatagcgat ttttaacgcg aaaaatgggc aacgcttgaa cagcaccttg
     5341 atcaatccag cgggtgggaa tttgggtttc tcgagtaatg ccaactttta aaccggaagt
     5401 attcgccaga taaacaatat gcggaataaa acaatggatt tttccccact cgggttctcg
     5461 acatgtgcct agatggaaat gacatttttc aggtttaaca atacaaaaat cgcatcgcgc
     5521 caatgtttgc gcacaaggaa aacaataacc ctgactgtaa ctttttttga tttttcgttg
     5581 acaatgcaca cactgaatat ctcctaaaaa cttcagcgtt acaattttac ctaaacaacc
     5641 attgagcggc agctcaactt cgccaattgg taaataatat tgcacgggat cacttaacgt
     5701 actttttaat ttacgcaagt cgcctgtata caaattagag ttccaccatt tcaaatccac
     5761 gcattttaac aacgccctcg taaattttat ggaacttcgg agatggatga caccaatctc
     5821 cctcggctaa aaacctaggc aaaatggttg aatttttttg tatttcggcc aaactaaaat
     5881 acttgcaaaa ttgaacactt ccgaattcat cgttccaatt atcagaaata gcgccctcta
     5941 agaccactgt ttcaaaataa agatgtatgt tatgaacgtg attttcaata tcaaaacact
     6001 ctaatacatg acgcaataaa cccgctttta caaccaagcc cgtttcttca tatacttctc
     6061 tttctacaca cgcagttaag gtttccgagt tttctatttt tccgcctggc agataccaat
     6121 aggtgccttc atcgctcaca aacaagagtt ttttctgctc gataattaac gcacgtgctg
     6181 tcacacgcaa tcgcggttga gtgctcattt tattacctta ggatgtttat gatacgattc
     6241 ccttaaaaac aaactgagga tacctgataa gaccaaacct aaaggcaaca cggccaatgc
     6301 aattgaaaaa tcatacacag tcaaaaccgg actattatta acaacgagga tcgcgtcatc
     6361 taaaattttt ccaatcaagg gtggtaacaa tacgccacct accatcacca ccatattggt
     6421 aaacgcaatc gctgttgcac tcaccgaatg gcgtgaaata tcgttactca ctgcaaaaat
     6481 taagatctct accgatgcaa aaaagctcga taaaaataat aatgaataca tccaaatcaa
     6541 cgatgtcgtt ggcataaata caactaaggc cataaagctt gccgcgagaa atgagccgat
     6601 gatcagtggg attcgccggc tttgcatcgc atctgaaata atgccccaca acggtgcacc
     6661 caccgcaaat cctaaaaaca gcattgaagc acctgtagct gcatcaaatt tagacaaacc
     6721 aacggtttct aaaaaactca tggcccaaat ttcggcaaaa cctgtaatcg gcaaaaaagt
     6781 taaaagaccg attaatcctg taatccacat ttgagaacta cagatgattt gtttaagacc
     6841 agtcagtttt aggtgcactt catgtgccaa atccatttcg ctaggatcct gtttttcgtg
     6901 acgggtccca tcccgtaata caagccacaa tataagcatc agtacaatac ccgctgctgc
     6961 tgaataatat aaagttgatt gccaacccat gcgtaacatc aaatgactca ttaaaactgc
     7021 acagctaatc ccgccaaaca tccctaaagt cgtacacagc cccgccataa acgcaaaata
     7081 ttttttaggt aaccaaacat ttgaaagttt aagaacgccc acatacgcaa atgccgatcc
     7141 aaaccccaat aaaaaccgac ccattgctgc aatccaaaat tgtccggtta taccaaaaag
     7201 caccgttccc aatacacaga gaaaacaggc aaatgttaag aggcgtcggg gcccaaaacg
     7261 atccatcatc ataccaaccg ggatttgcat gggtgtatag gcgtaataaa aaaatgcagc
     7321 caaatttcca aaggctgctt cacttaagtt aaaagtcaat tttaattcac tactcataac
     7381 actgggtgca acgcgtaagt aatattcgta ataataaaag aggattgcca atacacacat
     7441 ggtgacggcc gaacctgtca ctgcttttgt tctatctttg gttttattca ttggttttat
     7501 ccagtatttt attgcgtttg ttgtaaacat tggtcggggc gagaggattt gaacctccga
     7561 ccacatacac cccatgcatg tgcgctacca ggctgcgcta cgccccgaag gtttgaataa
     7621 taacacccca agctatattc gtcatctccg caaaatacca agattgtagg ataacactga
     7681 aaaacatttg cttgttaaat aactatctta agatgagata aaaagcatct atacttatga
     7741 acagaacgcc agcagatccg caaagggaga tcctcagttg caagaagaac tcgaaaccac
     7801 taaagcttcc aaactaaaat ttcgtcaaga agcattatta gagctccatg cacccgatca
     7861 aatcgacaag ctgattgagg tagtatctcc caaagcatgg attatgcttc tatcttttta
     7921 tatgctgctg attagcctca ttgtttgggt gatttttggg agtattccta cccgtgtcga
     7981 aggcaagggt attctattgg ttgaaaatgg gtctgtctac aatgcggttg ccccaccagg
     8041 tggcgggcaa attgccgata ttttagtgca gcaagaacaa gaaataaaaa aaggagatat
     8101 catcgcgcga ctggaacgac ccgatctaac agaaaagttg caacttgcca aaaattatgt
     8161 agaacagcta aaacgtgagc aagaggaact cagtcaaact tcaaaaaaag agctcgcgat
     8221 acgacatgaa aaaattagtc aacaaatatc tatgttggat agctctcttg cctcgggagc
     8281 taaaaacctc aaagatattg aaacgctgct taatttaaaa gaagaaaact ttaagaaagg
     8341 tatactgata tggcaagaca ttgaaaacac tcgccgcgat tattactctg ctaaagatcg
     8401 cttggagcaa cttcagaacc aaataactca actgaaaacc caagaagatg actttaaaga
     8461 tcaatggcga caacgtttaa aagataaaga actcaccatt cttgctgaac aacttaaaat
     8521 gaataatatg gccgcagaag tgacggtctc aaaaacggtg gttagcccag cagacggaat
     8581 tatcattagt gttaatacat ccgtcggtaa aatggttcca gatggcggct ctgttgtgac
     8641 cattacaagt ttaggagaag gtttggatgc attgatattt atgctgccac acgagggaca
     8701 acgggttacg cccgaaatgc ctgctttgat aaccccaacc accatcgaaa aagaagaata
     8761 cggcagcatg aaagggaaag tcttaagtgt atctgaattt ccacaagcag cagaaacaat
     8821 tattgcgcta ttacataatc aagaaatggc caaacaattt gtagaatcgg gtgcccctac
     8881 aggtatccga attcgtatta cgcgtgatcc taacactttc agcggttatc aatggtcctc
     8941 atctaaaggc ccaaataaaa aaatcagccc ggggacatta gcagaagttc gtattacagt
     9001 ccgagaacaa ccgcctatca gcttggttat tccagcattt
//
LOCUS       DAORVT010000007         8743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_4494_length_8743_cov_3.519222, whole genome shotgun sequence.
ACCESSION   DAORVT010000007 DAORVT010000000
VERSION     DAORVT010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8743
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4494_length_8743_cov_3.519222"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /gene="aqpZ"
                     /locus_tag="PK389_00300"
     CDS             complement(<1..507)
                     /gene="aqpZ"
                     /locus_tag="PK389_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005419605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HQY22183.1"
                     /translation="MKKVIAEFIGTFWLVLGGCGSAVLAAAYPELGIGFVGVSIAFGL
                     TVVTMAYAIGHISGCHLNPAVSIGLWMGGRFDKKELLPYMAAQVLGGIAGAGILYIIA
                     SGKAGFEIGGFASNGYGDHSPGGYSLTAALVTETIMTFMFLMIILGATHSKAPKGFAG
                     LTIGLGLTL"
     gene            complement(828..1865)
                     /locus_tag="PK389_00305"
     CDS             complement(828..1865)
                     /locus_tag="PK389_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22184.1"
                     /translation="MGQDEASKAHHCRKTNVVVHLKELMKQIFCLILLILSCAPAIAD
                     IAPNPIVVNSIYTVADCKIQMTSEYVFADIYNDSAKVECTFNLENFGDATEIQIGFPE
                     MNFQYWSMGKYTPTDRGNFRIYVDERLLTEKDIQVPAEMDSVYRKYMAVYHFDNEYQR
                     KRDSIYAANGIKEKGNRTIYPPGTYQKTQKALQDLFEWRESKPSLGSKLWIEFKEQQE
                     KGNFPWYVWNVKFDRQAKRQIKIVYTLPSGLGYGGDFRYFNYILRTGSGWYKSIGKAS
                     IKIQLHDIDIETIEQVAPSIFSIDSTQKTIQWTFTDLEPTAQDDIYLRYYNKAERRKW
                     DSAKRRRKRGW"
     gene            complement(1967..2815)
                     /locus_tag="PK389_00310"
     CDS             complement(1967..2815)
                     /locus_tag="PK389_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22185.1"
                     /translation="MTYFVGFPTFDRIHENVEAAMTIFSHRMIVISTIPMFLNAIFGV
                     MLLKFSDRYFPRIVIWISILLCSVSLISNLLLISSYSDLHTTGFTGEAKSWIIFMSAN
                     FQIIPLSIQIFLAFWLLNNHLQDTMWFGRWLFILLFSFTFFALGTDFVEKYDNYPIWA
                     TVGEKDWIEFRHATVSQAFIGVYLLPAILPLLFVLPMFWRRPPGVSKLLMTIFILIMI
                     WVSVITGNYFMPKLQAPLWSAYSKPLIEELMRNDLPLRVLPLLIMQTITAIMFFKIGL
                     HKIDRT"
     gene            complement(3354..3725)
                     /locus_tag="PK389_00315"
     CDS             complement(3354..3725)
                     /locus_tag="PK389_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HQY22186.1"
                     /translation="MLSDLDRFYLNQDEPNRSCMLAMRSMIIAQSRHITETVKYGMPC
                     FCYRKKVFCYLWTDKKTHEPYLLMVEGKHLDHPQLEAGSRSRMKIFRVDPRKDLPMIT
                     IDHILQQALNLYKSGVIKIKG"
     gene            complement(3760..4152)
                     /locus_tag="PK389_00320"
     CDS             complement(3760..4152)
                     /locus_tag="PK389_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY22187.1"
                     /translation="MIKFAYTILYVQDVTRSVTFYETAFGFARKFISPDNDYGELLVG
                     ETTLSFASIKLAGSNLHDGFIESSLDNKPFGIEIGFTTLNVEETLNNAIAAGATLVEK
                     PKTKPFGQTVAYVRDPDGFLVEICTPME"
     gene            complement(4203..4829)
                     /locus_tag="PK389_00325"
     CDS             complement(4203..4829)
                     /locus_tag="PK389_00325"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY22188.1"
                     /translation="MTKLKPEFWESVFSEKQEMWGFEPSISAMLTRDLFVSQSLKNIL
                     IPGIGYGRNARIFLEQGMAVTGIEISKTAIALAEKHFGTGMIIHHGSVTNMPFDHQQY
                     DGIFCHALIHLLDSDERAKLIRDCYHQLAPGGYMVFSVISKKAHTYGQGVFVTEDRYE
                     IFDGVKMYFYDQSSVHSAFDKAGLYEITEVQDVFPFFLIKCRKTKDAP"
     gene            complement(4889..5452)
                     /locus_tag="PK389_00330"
     CDS             complement(4889..5452)
                     /locus_tag="PK389_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HQY22189.1"
                     /translation="MRKLIAGLNMTLDGYCDHTAGIADEEIHEHYNDVLRNAGTLIYG
                     RITYQLMESYWPTIVKDPTGNKPMDDFAVLIDDISKIVYSQTLNDVSWKNSTLKKEIT
                     REDILVLKQQPGKNILVGSPSLIVALSNLGVVDEYQIGVQPIILGSGLPLFKNIRERI
                     DLNLLHSKTFKCGAMMLYYAPGPSRVV"
     gene            complement(5810..7771)
                     /locus_tag="PK389_00335"
     CDS             complement(5810..7771)
                     /locus_tag="PK389_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HQY22190.1"
                     /translation="MVTQPAFAQKRGQARIDSLLLDLQPTKTDTQQAKVLIDLSFTYN
                     SIDPDKGISYGHQGLAIAEKLNWKAGVVDAHRAIGVNHGFGKSEYSESLAAFSKSLAA
                     AVETGDQADAAKALNNMGVVYWYLSDFPKAIEHYYKALQIHEKMGNKVEMANSLSNIG
                     LVYNSQEDYPKALEYILKGNELDEELGNKTGIASNLGNIGQIYAAMDNLPKALEYDSS
                     ALALYTELGDKNGIARNLGNMGGIYAEQGLYTKSLDHYTQALAISKELGLQIGIATNL
                     GAIGGTYLKVAKDKNPEALRTMFNGNKSNALQQAQIYTDSAIVISKEIGDIHSLIKLY
                     ERQSEIQSLSGNYPGALENYKLYTISKDSVFNMEKDKKLTEATMQYEFDKKEAKAKAE
                     QEQKDIRQRAIRYSILAGLIGALIFAGVVYRQRIKIGQEKKISDAEKRRSDELLLNIL
                     PEEVAEELKTTGSAKAKAFTMVTVMLTDFKDFTTISEKISAELLVAEIHHCFSAFDRI
                     IQKYKIEKIKTIGDAYLCASGLPVSNYSHATEIVRAALEMRDFMHRRKMEKEALGEMP
                     FEIRIGIHTGPVVAGIVGVKKYAYDIWGDTVNLAARMEQNSEAGKINISENTYELVKD
                     RFDCTYRGKIEAKHKGMVNMYFVEPEHIS"
     gene            complement(7722..8024)
                     /locus_tag="PK389_00340"
     CDS             complement(7722..8024)
                     /locus_tag="PK389_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22191.1"
                     /translation="MHTADLSAKREGPGVSCQKNQKGSVPTEKSFDPSFRKSNSHRRS
                     LGKKVTGVSSEKNQEIAEPPISLYFVSPNETTTTPDLHPLHGYSTCLRAEKRTGAH"
     gene            complement(8228..>8743)
                     /locus_tag="PK389_00345"
     CDS             complement(8228..>8743)
                     /locus_tag="PK389_00345"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQY22192.1"
                     /translation="IIACTTLFLSCKKDGNSNVLNGCCDTPAINATVGLGHVYVPNVF
                     TPNGDGINDRLVVNGDQNIILIRSFRVSDKEGTTVFYAEDVTPNDPTNAWDGTVNAKY
                     KNGVYSIVMVVEAFDNATATLQGKVCNYRCLDAGVEDPISGAGCQFPAQVLNGVYDPS
                     LPSVESSGCFE"
BASE COUNT         2355 a         1880 c         1825 g         2683 t
ORIGIN      
        1 aagtgtcaat ccaagtccaa tagtcagtcc tgcaaaccct ttgggtgctt ttgaatgcgt
       61 tgctcctaat atgatcatta agaacataaa ggtcataatg gtttctgtga ccagggcggc
      121 agtcagacta tatccacctg gtgaatggtc tccatatcca ttggaggcaa atccaccaat
      181 ttcaaatcct gcctttccac tggcgataat gtaaaggatg ccagctccgg caatacctcc
      241 caatacctgg gccgccatgt agggaagaag ttctttcttg tcaaaacgac cacccatcca
      301 cagtccgatc gaaacagcgg ggttaaggtg acaaccggaa atgtgtccga tagcataggc
      361 catggtcact accgtcaagc caaaagcgat tgaaacacct acaaatccaa ttcctaattc
      421 aggatatgct gcggctaaaa cagcacttcc acatcctcca aggactaacc agaaggttcc
      481 gataaattct gctattacct ttttcatttt cgttgtgttt ttaagttagt ataaagaacg
      541 gtctgttttc tagaatagcg acgagggatg ctcgtagaca ggccgactga gttcgggttg
      601 tctccggggc atcttcctcc aaatttactt ttttctttta gttgacgaag ctaagttttg
      661 tttttgtgtc ggtagcgaag gcgaaccttt ggcctttgta gcttgtggtg gacatagatc
      721 gaaagccagc gaaggaggca ttctcaattg agattgccct gcttatgagc atccgatatt
      781 agttcatgtc attattgata aaatcgcttc aggggattga aatagtatta ccagcctctt
      841 tttcttcttc tctttgcact atcccatttg cgtctttctg ctttgttata ataacgaaga
      901 tagatatcgt cttgcgctgt tggttctaaa tccgtaaagg tccactgtat ggttttctgt
      961 gtggaatcga tgctgaaaat acttggtgca acttgttcaa tagtttcaat atcaatatcg
     1021 tgaagttgga tcttaatgga tgcctttcca atgcttttat accaaccgct accagttctt
     1081 aagatgtaat taaaatatct aaaatctcca ccatatccaa gacctgatgg caatgtgtat
     1141 acgatcttta tttgcctttt tgcttgccta tcgaatttta cattccaaac gtaccagggg
     1201 aaatttcctt tttcttgttg ctctttaaac tcgatccaaa gtttggagcc aaggctgggt
     1261 ttggattctc tccattcaaa tagatcttgc aaggcctttt gagtcttttg gtaggttcca
     1321 ggtggataaa tggttctgtt tcctttttct tttattccgt ttgcagcata gatactatct
     1381 cttttccgtt ggtattcatt atcaaagtgg taaacagcca tatactttct gtatacactg
     1441 tccatttcgg cgggtacttg aatatccttt tcagtgagca acctttcgtc aacatatatt
     1501 ctaaaattac cccgatcggt tggagtatac ttgcccatgg accaatactg aaaattcatt
     1561 tcaggaaacc caatctgtat ttcagtggca tcaccaaaat tctcgagatt aaacgtgcat
     1621 tcaacttttg ctgagtcatt gtatatgtct gcgaatacat actcactcgt catttgaatt
     1681 ttgcaatcag cgaccgtgta aatactattg actacgatgg ggttaggagc gatgtcagca
     1741 attgccgggg cacatgataa aattaatagt ataagacaga atatttgttt catcagttct
     1801 tttagatgga ctacaacgtt tgtctttcta cagtggtgcg cttttgaagc ctcgtcttgt
     1861 cccacgaaac taaacgttga acgaaaataa aacttaaaaa acaagttgtc acggtaccat
     1921 tgcagaaaca ttttgttatg gctttgtacg catcaaccaa tctattttaa gtcctatcaa
     1981 tcttatgtag tccaattttg aaaaacataa ttgctgttat agtctgcatt atgagcagag
     2041 gtagtactct caggggtaaa tcatttctca taagttcttc aattaatggc ttagagtagg
     2101 ctgaccacaa aggagcttgc agttttggca taaaatagtt acccgttatt accgataccc
     2161 aaatcataat caagatgaaa attgtcatta acaatttaga aactccagga ggcctccgcc
     2221 agaacattgg taaaacaaat aaaagtggca aaatggctgg taatagatat actccaataa
     2281 atgcctgaga cacggtcgcg tgccgaaatt caatccagtc cttctcacca actgttgccc
     2341 aaatcgggta gttatcatat ttctcaacga agtctgtccc caatgcgaag aatgtaaatg
     2401 agaacagtag gataaaaagc catcgaccaa accacattgt atcctgcaag tgattattta
     2461 atagccagaa agcaagaaaa atttgaatgc ttaaaggaat aatttgaaag ttcgcgctca
     2521 taaaaataat ccaacttttc gcttctccag tgaaaccagt agtatgaagg tcagaataac
     2581 tcgatataag tagcaggttt gaaataagac taactgagca aagtaggatt gaaatccata
     2641 ttacaatcct aggaaaatat ctatcactaa atttcaatag cataactcca aaaatcgcat
     2701 taagaaacat tggaatagtt gaaataacaa tcattctgtg gctgaaaatg gtcattgcag
     2761 cttctacatt ttcatgaatt ctgtcaaatg ttggaaagcc aacaaaatac gtcataagca
     2821 atacaccaaa agaatagaac aatgttacaa agtagattaa cagtgtaaga atggtcagtg
     2881 aagagttgtc ccggttaata ttcataaatt attttgttgt cgctgtgtta atatttcata
     2941 atttccaact actcgtcctg gcgtatgagc cataacacgg atatgtccgc aaccaaagat
     3001 aggagtttcc tctgattctc agcacctaca agttgcgttt tcccatatgt tcattgcggc
     3061 aagccaaagt tgcacttttt aaatatcccc ttactccttg aactcgggac ttcacctcaa
     3121 ttcatttcag atattgacag cccacaggat caacattgcg tttacatgca cttgactaag
     3181 cttataaatt gtgtgatgag gttgtgacaa ttttcttcgt tcacgattct ttaagtagca
     3241 tggccctttg gttttattcg ttttgtggat tgatgaagct aaaatgtatt tccttgttgg
     3301 aaccaaacaa agagcgtcat ggaaatacga atctgcattt cacgttgcct atatcacccc
     3361 tttattttta taaccccgct tttatacaaa ttcaatgcct gctgtaagat atgatctata
     3421 gttatcatcg gcagatcttt gcgtgggtct accctgaaga tcttcatgcg ggagcgtgag
     3481 cctgcttcca gttgcggatg atccagatgc tttccttcga ccatcaagag atagggttcg
     3541 tgcgtttttt tatcggtcca cagataacaa aacacttttt tccggtaaca gaagcaaggc
     3601 atgccatact tcaccgtttc ggtaatatgc cggctctggg cgatgatcat actgcgcata
     3661 gcgagcatac aacttctgtt gggctcgtcc tggtttaaat aaaagcgatc aagatcagac
     3721 aacatgcaac taaattacat gattaataat gtttcaccct cactccatgg gcgtacaaat
     3781 ctcaactaaa aaaccatcag gatcccgcac ataagccacc gtctgtccaa atggtttagt
     3841 tttgggtttt tcaaccagtg tggcacctgc ggctatcgca ttgttaagtg tttcttcaac
     3901 attgagtgtt gtgaatccta tttcaatacc aaagggttta ttgtccagac tgctttcgat
     3961 aaagccatcg tgtaggtttg agcccgcaag ctttatggaa gcaaatgaaa gggtagtttc
     4021 accaaccagg agttcaccat agtcattatc gggtgaaata aatttacgtg caaagccaaa
     4081 ggctgtttca tagaatgtaa cggatctggt cacgtcttga acgtaaagga tggtgtaggc
     4141 aaatttgatc atagatttgg gttttttaca gaaaataata tcagcctgtg tctataatgt
     4201 attcaaggag cgtccttagt cttcctgcat ttgatcagaa agaatggaaa cacatcttgt
     4261 acctcagtaa tttcatacag tccagctttg tcaaatgctg aatgaacaga tgactgatca
     4321 taaaagtaca ttttgacgcc atcaaatatc tcatatcgat cctcagtgac aaacacgccc
     4381 tgaccgtagg tatgtgcttt ttttgaaata acggagaaga ccatataccc acctggagct
     4441 aattgatgat agcaatcacg gatcaattta gcccgttcgt cactatccag caggtgaatc
     4501 aatgcatgac aaaaaatacc atcatactgc tgatggtcaa acggcatatt tgtaacagaa
     4561 ccgtgatgga tgatcatacc agtgccaaag tgcttttcgg caagggcaat agccgttttt
     4621 gatatttcaa ttccggtcac cgccatgccc tgctccagga atatccgggc gttacggccg
     4681 taaccaattc ctgggatcag tatattcttc aacgattgac tgacaaacaa atccctggtc
     4741 agcatagctg atatagaggg ttcgaatccc cacatctctt gcttttcact aaagacagat
     4801 tcccagaatt cgggttttag cttggtcata tttcatgtgt aatcgccatc aatttacgcc
     4861 ctttccaaaa ttcttttacc tgccataact aaacaacccg tgacggtcct ggtgcatagt
     4921 acagcatcat cgcaccgcat ttgaatgttt ttgagtgtaa gaggttaaga tctattcgtt
     4981 ccctgatgtt tttaaacaaa ggcaatccac taccgagaat gatgggctgg acgccaattt
     5041 gatattcgtc aacaacacca aggtttgaca aggctacaat tagagaggga ctacccacta
     5101 aaatgttttt ccctggctgt tgtttgagta ccagtatgtc ttctctagtg atctctttct
     5161 tcaaggttga gtttttccaa ctaacatcat tcagtgtttg tgaatacacg atcttggaaa
     5221 tatcatcgat cagtacggca aaatcatcca tgggtttatt gccggtagga tcttttacaa
     5281 ttgtgggcca ataactttcc atgagctggt aggtgatccg gccgtatatc agtgtgcccg
     5341 cattgcggag gacgtcatta taatgctcat gtatttcttc gtctgcgatg ccggctgtat
     5401 gatcgcaata cccgtcgagg gtcatgttga gtccggcgat taattttctc atcttagaag
     5461 taaatggaag tgaatctttt attgtttcag caagggttca gatgaataca gttgtacaac
     5521 aagatgcaaa atattccaga tttagcaatt tactaactct tgaataattg aatcaaatga
     5581 tattctgcgg atcctgcctg agcaaaggat gattgtgcct gacgttgaaa acgtctgcac
     5641 agactattac ccggagaagg tttttttgat ttttcgtacg cgatcttaag tcaacttaag
     5701 ctcaactttg tccttttaca gcggcctgcg gccactatta gggcatgtag gcattgagat
     5761 aaatctttcg tggtggtacc ccctttcagg gggcaaaggg ggtcagttct caactgatat
     5821 gctccggctc cacaaaatac atattgacca tccccttgtg cttggcttcg attttgccgc
     5881 ggtaagtgca gtcaaaacgg tctttcacta gttcataggt gttctcgctg atattgattt
     5941 ttccagcttc actgttttgt tccatgcggg cggcgaggtt gacggtatcg ccccagatat
     6001 cgtaggcata cttcttcaca cccacgattc cagcgacgac agggcctgtg tggatgccaa
     6061 tgcggatttc aaacggcatt tctccgaggg cttctttttc cattttgcgg cgatgcatga
     6121 aatcgcgcat ttcaagggca gccctcacga tttctgttgc gtgtgaataa ttagataccg
     6181 gaagtccact cgcacagaga taggcatcgc cgatagtttt gattttttcg attttatatt
     6241 tctggatgat acgatcgaag gcactgaagc aatggtgaat ctcagctaca agtaattcag
     6301 cactgatctt ctcactgatc gtcgtgaagt ccttgaaatc tgtcagcatg accgtcacca
     6361 tggtaaaggc ctttgccttg gcggagccgg tagttttcaa ttcttcagca acttcttcag
     6421 gcaggatatt gagcagcagt tcatcactac ggcgtttttc agcgtcgctt atctttttct
     6481 cctgacctat cttaatacgc tgtcgataga ccacacctgc aaaaatcaaa gcaccaatga
     6541 gacctgcaag aatagaatac cggatggccc tttgcctgat atctttttgt tcttgttcgg
     6601 ctttggcttt ggcttctttt ttatcaaact catattgcat cgtcgcttcc gtcagctttt
     6661 tgtctttttc catattgaag accgagtcct tgctgatagt atacagctta tagttttcca
     6721 gtgcgccagg atagttgccg gacaaggatt gtatttcact ctgacgctca tataatttta
     6781 tcagtgaatg aatatcgccg atttccttgc tgatgacaat tgcactgtct gtatagattt
     6841 gtgcttgttg caaggcattg cttttatttc cgttaaacat ggttctcaat gcttccggat
     6901 ttttatcctt ggccactttc agataggtac cgccgatagc gcccaggttg gtggcaatgc
     6961 cgatttgcaa tcctagttct ttgctgatcg caagtgcttg tgtataatga tcaagtgatt
     7021 tggtatacaa accttgttcg gcatagatcc cgcccatgtt gccgagatta cgcgcgatgc
     7081 cgtttttgtc tccgagttca gtatacagcg ccagcgcgct tgaatcgtat tcgagtgctt
     7141 tcggtagatt gtccatggct gcatagatct gaccgatatt gcccaggtta gatgcgatgc
     7201 cggtcttgtt accgagctct tcgtcgagtt catttccctt gaggatatat tccaacgctt
     7261 tcggataatc ttcctgactg ttatagacta gtccgatatt gctgagcgaa ttcgccatct
     7321 ccactttgtt gcccatcttc tcatggatct ggagggcttt gtagtagtgt tcgatggctt
     7381 taggaaaatc agaaaggtac caatacacga cgcccatatt attcaacgcc ttggcggcat
     7441 ccgcttggtc accggtttct actgcagcgg ccagggattt tgaaaaggca gccaatgact
     7501 cggagtactc tgattttcca aaaccatggt tgacaccgat cgctctgtgt gcatcgacta
     7561 ctccggcttt ccagttgagt ttttctgcta tcgccagacc ctgatgtcca taggagatgc
     7621 ctttgtcggg atcgatggag ttgtaggtaa aactaaggtc gatcaagacc ttggcctgtt
     7681 gcgtatcggt tttggtgggt tggagatcca gcaggagcga gtcaatgcgc gcctgtcctc
     7741 ttttctgcgc gaaggcaggt tgagtaacca tgcagaggat gcaagtcagg agtagtagtg
     7801 gtttcatttg gagaaacaaa ataaagagaa atcggaggtt ctgcaatttc ttgatttttc
     7861 tcagaactca cccccgtcac ctttttgccg agagatctgc ggtgcgaatt tgattttcta
     7921 aaagaggggt caaatgattt ttcggtaggc acagatcctt tttgattttt ctgacaactc
     7981 acccccggcc cctctctttt tgccgagaga tctgcggtat gcatttgatt gtctgaagag
     8041 aggggtgaaa tgatttttca gtaggcacag atctgacata ttctttaaag gaaaatatca
     8101 aagcgctccc ctctctttta catagtccta ttcgtatgca aaatcatttc ttaagagagg
     8161 ggtcgggggt gagttctgca ggagcaatag aggggccggg gttgcgttat gcagaagcat
     8221 ggtagtttta ttcaaagcat ccactacttt ccactgaagg taagctggga tcatatactc
     8281 catttaaaac ctgcgctgga aactgacaac ctgctccaga gatcggatct tctactccag
     8341 catccagaca acgataatta catacctttc cttgcagcgt agcagtggcg ttgtcaaagg
     8401 cttcgaccac cattacaata gagtacacgc catttttata tttagcattg actgtaccat
     8461 cccaggcatt ggtaggatcg ttaggtgtaa catcttcggc atagaatact gtggtgcctt
     8521 ctttgtcgct gacacgaaag cttcgaatca atatgatgtt ttgatcacca ttgaccacca
     8581 gacgatcatt gatcccatct ccattgggtg taaacacatt gggtacatag acatgaccaa
     8641 ggcccacggt ggcattgatg gcgggtgtgt cacaacatcc gttgagtaca tttgaattgc
     8701 catctttttt gcaggaaagg aatagcgtgg tgcatgcgat gat
//
LOCUS       DAORVT010000008         8170 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_5032_length_8170_cov_3.871226, whole genome shotgun sequence.
ACCESSION   DAORVT010000008 DAORVT010000000
VERSION     DAORVT010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8170
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5032_length_8170_cov_3.871226"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PK389_00350"
     CDS             <1..306
                     /locus_tag="PK389_00350"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HQY22193.1"
                     /translation="LSTLRDAIRRESKIDIIYRSKKGQQSPCVIWPFAIGYFTEGRIL
                     VGWCEKEKNYRHFRTDGIISFTVLNERYTRRRESLFREWHSFQMESRSPTLKKGGKP"
     gene            complement(371..862)
                     /locus_tag="PK389_00355"
     CDS             complement(371..862)
                     /locus_tag="PK389_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22194.1"
                     /translation="MIIECTNPKEISFSYKEICNPTLLEYAKNKLSPQGILKQDDSGS
                     YCYLKIQDNFIFELFPFIKEPGLSIPDYFPPRYDTGAHISVIYPEEIFSEKINIEELA
                     ETFSFEPTGLLSVSVFNKTFFALAVSSPALKKLRLRYNLPTKLNYRGLLVPFHITIAT
                     SAR"
     gene            complement(864..1253)
                     /locus_tag="PK389_00360"
     CDS             complement(864..1253)
                     /locus_tag="PK389_00360"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HQY22195.1"
                     /translation="MPKTTNDLLQKIINQEKTAAEFGFYWENIEQLLEQIQSECAEVK
                     EAWLNQDNAHLKEEVGDLINATLSLCIFLKLDPFETLSESVEKFQQRYDALVKLVNQD
                     GLSHLKNKPLDILLDYWNKAKKVTGKI"
     gene            complement(1266..1958)
                     /locus_tag="PK389_00365"
     CDS             complement(1266..1958)
                     /locus_tag="PK389_00365"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HQY22196.1"
                     /translation="MPFNKKSLTSVIIASFVLMFYSFCCVADIQTESAFTSQMLLQNA
                     IKAGQLDDVRILLEQGGIDVNQPFDQGITPLHSAVINNQENIATILIQNGAKIDAEDT
                     STKATPLHLAALYGRENIANLLIQKGANVNAVMKFGITPLLVASQFKHAQIVQLLLEN
                     KTDVNHADDEGFTALHFVAQNGDPVIAEMLVEHKAELNLLDKTHKSTPLAIANQNNHF
                     DIAQMLQKHGGQ"
     gene            complement(1993..2835)
                     /gene="murI"
                     /locus_tag="PK389_00370"
     CDS             complement(1993..2835)
                     /gene="murI"
                     /locus_tag="PK389_00370"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HQY22197.1"
                     /translation="MLQSSTHPIGIFDSGMGGLTVAKTITEYMPNENIVYFGDTAHTP
                     WGDKSTAAIQAYAIKICDVLLQHQCKLIVIACHTASTAAYELIAEYVGNKAFVINVVD
                     PVIKHIQQHHSNKKIGLIGTKQTIRSNTYKRRVDRLDQNIELASLATPLLAPLIEEGF
                     GEKHVTTTIMQEYLAHPNLENIEALILGCTHYPLLKPMIQNFYKNKVDIIDASEMTAK
                     ILKEHLTLHNLLNPGTVSKETTVNGDASKRTFYVSDHNEFFETIAKLFFPGKIELKHY
                     PLWE"
     gene            2926..3171
                     /locus_tag="PK389_00375"
     CDS             2926..3171
                     /locus_tag="PK389_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22198.1"
                     /translation="MWHLKTRLGVFWVVPMSSSANKFLLGVNDEELGLYTDVERAAKD
                     VHDQATGFLKWDIQSTVRAPEHIREWAEGEPKSWDKH"
     gene            3259..3519
                     /gene="rpmE"
                     /locus_tag="PK389_00380"
     CDS             3259..3519
                     /gene="rpmE"
                     /locus_tag="PK389_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010548152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HQY22199.1"
                     /translation="MKPDIHPQYASIQITCSCGHVFNTGSALGKDLNVEVCNKCHPFY
                     TGKTKIVDTAGRVDKFSKKFGGFALNKNQSSDDKKSGENAED"
     gene            3563..4858
                     /locus_tag="PK389_00385"
     CDS             3563..4858
                     /locus_tag="PK389_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005222922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HQY22200.1"
                     /translation="MVEALKATDEALSKAAEDAFNKAALDYHEKPTPGKLSINITKST
                     ESQRDLALAYTPGVAVPVLAIAANPTDAYKYTAKGNLVAVITDGTAILGLGNLGALAS
                     KPVMEGKAVLFKRFADVDVFDIEVDAKTPETFIETVVNIAPTFGGINLEDIKGPECFE
                     IERALTERLNIPVFHDDQHGTAVIVAAALLNALELQKKSLQDIKLVCLGAGAAGIATM
                     RLLVSLGLHRTSITMVDTQGIIHVGREDLNPYKFAFAQETEKRTLTEAMTGADVFIGV
                     SGPNAVTGEMLQLMAPNPVVFALSNPDPEIKPEIAHQARHDLVMATGRSDYPNQVNNV
                     LCFPYIFRGALDVRARCINQAMLIAVVHAIRSLVHGVTPESVVKAYADTENLTFGPQY
                     ILPKPMDPRLLQTVPAAVARAAIESGVAQMPYPDHYPIL"
     gene            4896..5855
                     /gene="mdh"
                     /locus_tag="PK389_00390"
     CDS             4896..5855
                     /gene="mdh"
                     /locus_tag="PK389_00390"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HQY22201.1"
                     /translation="MRKPKIALIGAGNIGGTLAHLALMKGLGDVVLFDVAEGVPQGKA
                     LDLLQCGPIEGFDARITGTNDYKTIEGADVAIVTAGVARKPGMSRDDLLSINAKVMKS
                     VGEGIKQFCPNAFTICVTNPLDIMVRLLQDFSGVADNRIVGMAGVLDSARFRTFLAEE
                     FKVSIHEVQAYVLGGHGDSMVPLTGLSNVAGVTLEKLVSEKKITRERLNAIVDRTRKG
                     GGEIINLLKTGSAFYAPASSAISMAKSYLMDEKRILPCAAKLKPGQYGIKEPLFVGAP
                     VKIGSKGIEEIIEVELTTEERANLQVSIQAVIELNRAVAALNL"
     gene            5942..6766
                     /locus_tag="PK389_00395"
     CDS             5942..6766
                     /locus_tag="PK389_00395"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HQY22202.1"
                     /translation="MDTQKYVKRPMLAKPWIYFLATYIWTWSFFGIAYKMGVSAESGH
                     ALGVVLVLLALAGPAVMGIAFTYLTLNKEGQKDYWKRILDFKRISFKWYLVIFFLIPA
                     VSILAAMLNGYWDTYSFSHKLPSLYLTLLSVPLVPILEELGWRGYVLDRLQEKYSALT
                     SSLILGALWGPWHLPVFFLHGSIFGLMPFASLTFWLYMYHEIVISVCFTWIYNNTARS
                     TLSAILFHIVLEFCANTGLIPWDKAEHRYNVSLWTLFAIVIAIIYGTKTLARKEEH"
     gene            6918..>8170
                     /locus_tag="PK389_00400"
     CDS             6918..>8170
                     /locus_tag="PK389_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22203.1"
                     /translation="MMQCKLGRIVLFMVLSACYTEANAVELTIYSSAAPGTLSSNNSN
                     LNETLPGYAYVIDRRELTLQLGQNDIRYTHIPSYIDPTTVQFRSLTDVAGTEILQQKY
                     RFDLLNNQTLLESYLGKEITVEQGHGSDLGSFTGILLNTQNGLTLQDKNKQIVTVNNY
                     SAIRLSLIEDEGFKQPTLFWNIVAKKQGLHQTETRYQTKGITWWADYNALFEEKGDPN
                     SGTLELNSWVTVMNKTGLNFDSADLKLVAGDVNTAPPQQLPRANLMMAAKVGYSGSAG
                     FTEKPFFEYHVYALNGKISLANNATQQIKFLPKISKIPVEKLYIYNSNPGMLYTGYNN
                     TERYVNTSVPAKVDVYLKWKNTSRAGLGIPLPVGRVRISAISKSNKSPEFIGEDVIDH
                     TARDEEIQIKMGSAFDVSVEKKQTD"
BASE COUNT         2388 a         1469 c         1801 g         2512 t
ORIGIN      
        1 ctttcaacat tgcgagatgc cattaggcgt gaatctaaaa tagatattat ttaccgttca
       61 aaaaagggtc aacagagtcc gtgcgtcata tggccatttg cgattggtta ctttaccgag
      121 ggtcgcattc tcgtcggttg gtgcgagaag gaaaaaaatt atcgtcattt tcgcactgac
      181 ggcataatat ctttcacagt tcttaatgaa agatatacgc gtcgacgcga aagccttttt
      241 cgtgaatggc actcgtttca gatggaaagt cgttccccca ctttgaaaaa ggggggaaag
      301 ccgtgagagc tttgctctca tcggcagggg ggattttaat cggcgatcac ttttgctttg
      361 cttcttgaat ttaccgtgcg cttgttgcaa tcgtaatatg aaatggcacc aaaaggccac
      421 ggtaattcaa cttggttgga aggttatacc ttagtcgcaa tttttttaat gcaggtgaag
      481 aaacggcgag tgcaaaaaaa gttttattaa aaacagaaac actcagcaat ccggtaggtt
      541 caaaagaaaa agtttctgca agctcctcaa tgttaatttt ttctgagaat atctcttctg
      601 gatatataac gctaatatga gccccagtgt cgtatctggg tggaaagtaa tctggaatag
      661 ataaaccagg ttctttgatg aatggaaata attcgaaaat aaaattatcc tgtattttta
      721 gataacaata agatccagaa tcatcctgtt ttaaaatacc ttgtggcgac agcttgtttt
      781 tggcatattc aagtagtgtg gggttacaga tttctttgta agaaaaagaa atttctttgg
      841 ggttggtgca ttcaatgatc atgctaaatc tttcccgtca ctttttttgc tttgttccaa
      901 taatccaaca aaatatccaa aggtttgttt tttaagtgcg aaagcccatc ttgattgaca
      961 agcttaacta acgcgtcata acgttgttgg aatttttcaa cgctctcgct taatgtctca
     1021 aaggggtcaa gttttaaaaa aatgcataaa ctgagggtag cattgataag atcaccgact
     1081 tcctctttta ggtgcgcatt gtcttggttt aaccaggctt cttttacttc agcgcattcg
     1141 ctttgaattt gttccagaag ttgttcgata ttttcccaat agaagccaaa ttcagcagcc
     1201 gtcttttctt gattaataat tttttgtaat aaatcatttg ttgtttttgg cattatgatt
     1261 ctccgctatt gtcccccatg cttttgtagc atctgtgcga tatcaaagtg attgttttga
     1321 ttcgcgatcg ctagtggggt tgatttgtgg gttttatcca gcaaattaag ttcagcttta
     1381 tgttcaacca gcatctctgc aataacgggg tcgccgtttt gtgcaacaaa gtgtagtgct
     1441 gtaaagccct cgtcgtcagc atggttaacg tccgtcttat tttctaatag taattgaaca
     1501 atttgggcat gtttaaattg agacgcgact aagagtggtg taatgccaaa tttcataacc
     1561 gcatttacat tggcaccttt ttgaattaat agatttgcaa tattttcccg tccataaagt
     1621 gcagctaaat gaagaggagt tgctttggtc gaagtgtctt ctgcatctat ttttgcacca
     1681 ttttggatca gtatggtggc aatattttct tgattattaa tgacggcaga atgtaagggt
     1741 gtaatacctt gatcgaacgg ctggttaaca tcgatacctc cttgttccaa taaaatgcgc
     1801 acatcatcca actgccctgc cttgattgca ttctggagta gcatttgtga agtaaatgca
     1861 ctttcagttt gaatgtcagc gacacagcag aagctgtaaa acattaaaac aaaagaagca
     1921 ataataactg aggttagcga ttttttatta aaaggcataa aatttctcct gtaaggcgca
     1981 tagataaaaa aactattccc acagtggata atgtttcagt tcaatttttc ctgggaagaa
     2041 cagttttgca atggtttcaa aaaattcgtt gtgatcagat acataaaaag ttcttttaga
     2101 ggcgtcgccg ttgaccgtgg tttctttaga gacggtcccg ggattcaaaa gattatgtaa
     2161 agtcagatgc tcttttaaaa tcttagcggt catttcggac gcatcaataa tatcaacctt
     2221 atttttataa aaattctgaa tcatgggctt taaaagaggg taatgtgtac atcctaaaat
     2281 cagcgcttca atattttcta aattgggatg cgctaggtat tcttgcataa ttgttgtcgt
     2341 tacatgtttc tcgccaaacc cttcttcaat caatggcgcc agcaaggggg tcgcaagaga
     2401 agcgagctca atattttgat cgagtcgatc aacccgtctt ttgtatgtgt tcgaccgaat
     2461 cgtttgtttg gtgccaatta aaccaatttt tttgttgcta tggtgttgtt gtatatgttt
     2521 gataacggga tcaacaacat taatcacaaa agccttgttg ccaacatatt ccgcaatcag
     2581 ctcatatgca gcagtagagg cagtatgaca agcaatcaca atcaatttac attggtgttg
     2641 taaaaggaca tcgcagattt taatggcata agcttgaata gccgccgttg atttatcacc
     2701 ccaaggcgtg tgtgcagtat cgccgaaata aacgatgttt tcatttggca tgtattcggt
     2761 aatggtcttt gcgactgtca ggccgcccat gccactgtcg aagataccaa ttggatgcgt
     2821 tgatgattgc aacatagggt tacagtctat cacattattg ggataagcgt gtcctatact
     2881 taaggatata aaataggtta ttgatattgt cggaaggggg ttctgatgtg gcatttaaaa
     2941 accaggttag gcgtcttttg ggtggttcca atgtcttcca gcgcaaataa attcttgtta
     3001 ggcgtaaatg atgaagagct gggtctttat acagatgttg agcgagcagc caaagacgta
     3061 catgatcaag caaccggatt tttgaagtgg gacattcagt ctactgtgcg agcgcccgag
     3121 cacattcgag agtgggcaga gggagagccc aaaagttggg ataagcatta gtttaatgac
     3181 ttatgtttaa gtaggttgca gcgatgacct aatttcggta tgatccttga caagtacttt
     3241 tgatcgaggg tagacattat gaaaccagat attcatccac aatatgcttc aattcagatt
     3301 acatgtagtt gtgggcatgt atttaacaca ggttctgcct taggcaaaga tcttaacgta
     3361 gaagtctgca ataaatgcca tccattttat acgggtaaaa cgaagatcgt tgataccgca
     3421 ggtcgcgttg ataaattcag taaaaaattt ggtggttttg ctttaaataa aaatcaatcc
     3481 agcgatgata aaaaatcagg cgagaacgcc gaagactaag cttgatgtgt tatttccttt
     3541 ttttatttgg tggattttta atatggttga agcattaaaa gctacagatg aagctttgag
     3601 caaagcggca gaggacgcat tcaataaagc cgctttggat tatcatgaaa aaccaacacc
     3661 gggtaaatta agcattaata tcacaaaatc cacagaatct cagagagatt tagcgcttgc
     3721 ttatacgcca ggtgttgcag tacccgtact agcgattgca gctaatccca cagatgcgta
     3781 taaatataca gccaaaggaa atctggttgc ggtcataaca gacggaaccg ctattttagg
     3841 tcttgggaat ttaggtgctt tggcaagcaa accggtgatg gaaggcaaag cggttttatt
     3901 taaacgattt gcagatgtgg atgtttttga tattgaagta gacgcgaaaa ccccagagac
     3961 ctttatcgaa actgttgtga atattgcgcc tacttttggt ggcattaacc tcgaagatat
     4021 caaaggtcct gaatgttttg agattgagag agcgcttacc gaacgtctca atattcctgt
     4081 gtttcatgat gatcaacatg gtacggctgt tattgttgcc gcagcattat taaacgctct
     4141 ggaattgcaa aaaaaatctc tgcaagatat caaattggtt tgtttaggcg caggtgctgc
     4201 aggcattgca acgatgcgat tattagtatc gttgggctta catcgaacat cgattactat
     4261 ggtcgatacg caaggtatta tccatgttgg gcgcgaagat ttaaatccct ataaattcgc
     4321 ctttgcgcaa gaaacagaaa aacgcacttt aacagaagcg atgaccggtg ctgacgtctt
     4381 tatcggggta tctggtccca atgcagtaac cggcgaaatg ttacagttga tggcgcctaa
     4441 tccggtggtg tttgccttat caaatcccga tcccgaaatt aaaccagaaa ttgcgcatca
     4501 agccaggcac gatttagtca tggcaacggg tcgaagtgat tatccaaatc aagtgaataa
     4561 tgtgttgtgt tttccttaca tttttagagg tgcattagat gtgcgtgcgc gttgtattaa
     4621 ccaagccatg ctaattgctg ttgtacacgc tattcgatcg ttggtacatg gtgtaacacc
     4681 agaatccgtt gttaaagctt atgctgatac agaaaattta acattcggtc ctcaatatat
     4741 tttgccgaaa ccgatggatc cccgtttgtt acaaacagta cctgcagcag ttgcgagggc
     4801 agccatagaa agtggggtag cacaaatgcc atatccggat cattatccta tactataaat
     4861 tgtttgagta aaattgtttt agcaaggagt gcaacatgcg taaaccaaaa attgcattaa
     4921 ttggagctgg caatattgga gggaccctcg cgcatttagc gttgatgaaa ggattaggcg
     4981 atgtggtatt gtttgatgtt gccgaaggtg ttccacaagg aaaagcctta gatcttttgc
     5041 agtgcggccc gattgaagga tttgatgctc gcattaccgg tacgaatgat tacaaaacaa
     5101 tcgaaggcgc ggatgttgcc attgtaactg cgggtgtcgc tagaaaacca gggatgagtc
     5161 gagatgatct gctgtctatt aatgccaagg tgatgaagtc tgttggcgag ggcattaagc
     5221 aattttgtcc aaatgccttt actatttgtg ttaccaatcc gctagatatc atggttcgtt
     5281 tattacagga tttttcgggt gttgccgata atcgcattgt cggtatggcc ggtgttttag
     5341 attctgcgcg atttagaact tttttagcag aagaatttaa agtgtctatt catgaagtgc
     5401 aagcctatgt attaggcggg catggcgata gcatggtgcc cttaacaggt cttagcaatg
     5461 tcgctggcgt gactttagaa aaattggttt cagaaaagaa aattacgcgc gagcgtttaa
     5521 atgcaatcgt ggatcgcacg cgcaaaggcg gtggcgaaat tattaatctt ttaaagacag
     5581 gctctgcttt ttatgcgccc gcctcttctg caatttctat ggcaaaatcg tatttgatgg
     5641 atgaaaaacg catacttcct tgcgctgcca aattaaagcc aggtcagtat ggtattaaag
     5701 agcccttgtt tgtaggcgca cccgttaaaa ttggcagcaa aggcattgaa gaaattattg
     5761 aggttgaatt aaccacagaa gaacgtgcca atttgcaagt ctccattcaa gcagtgattg
     5821 agcttaaccg agcggttgcg gcacttaatc tctaaacttg ttttctgttg acaaatgata
     5881 gcttgtttac gagacgcttc cattgatccc tgatttatga ctaattgttg ggaattaagg
     5941 tatggataca caaaaatacg taaaaagacc aatgcttgct aaaccctgga tttatttttt
     6001 agccacttac atatggacat ggtcattctt tgggattgca tacaaaatgg gggtcagcgc
     6061 cgagagcggt catgcactgg gcgtggtttt agttttattg gctttagctg gcccagcagt
     6121 aatgggcatt gcttttacgt atctaacact caacaaagaa ggccaaaaag actattggaa
     6181 gagaatacta gattttaaac gaatatcttt taaatggtat ttagttattt tttttcttat
     6241 acccgctgtc agtattctag cagcaatgct aaatggctat tgggatacgt attcattttc
     6301 gcacaaactt ccatcgctat atttaacact attatcggtt ccgcttgtgc ctattctgga
     6361 ggaactgggt tggcggggtt atgtgctgga tcgtctacaa gaaaaatata gtgcgctgac
     6421 atccagtcta attttaggtg ctttgtgggg accatggcat ctgccagtat tttttttaca
     6481 tggctctatt tttgggctca tgcctttcgc gtcactgaca ttctggctat acatgtatca
     6541 tgaaattgtg atctctgttt gttttacttg gatttataac aataccgctc gcagcacatt
     6601 atcagctatc ttatttcata tcgtacttga attttgcgcc aataccggtc ttatcccctg
     6661 ggacaaagca gaacaccgtt acaatgtgtc gttatggact ctctttgcaa ttgttatagc
     6721 cattatttat ggtactaaaa cactagctcg taaggaagag cattagaggc cagatcttga
     6781 ttatgtatta tttagggtgc agaataactc taaagaatgg gtaaaaagta tagaatcaag
     6841 atctgacctc ttaaactggc ttacttggtc tagaattaag agaagaccta agtagatatc
     6901 caaggaaagg aatggctatg atgcaatgca aactcggtag aattgttttg tttatggtcc
     6961 taagcgcttg ttatacggaa gccaatgctg ttgaactcac aatttatagc agtgcggcac
     7021 ctggaaccct ttcttctaat aattctaatt tgaatgagac tttacccggg tatgcctatg
     7081 tgatcgatcg cagggagttg acattacaat taggtcaaaa tgatattcgt tatacccata
     7141 ttccgagtta tatagatccg acgacggtgc aatttagatc tttaaccgac gtggcaggta
     7201 cagaaatttt gcaacaaaaa tatcgctttg atttattgaa taatcaaaca ttattagaga
     7261 gttatttagg aaaagaaata accgttgagc aaggtcatgg ctccgatttg ggttctttta
     7321 caggcatatt attaaatact caaaatggtc ttactttaca agataaaaat aagcaaattg
     7381 tcacagtaaa taattattct gccattcgat tgtctttgat agaagatgaa ggattcaagc
     7441 aacccacgtt gttctggaat attgttgcaa aaaaacaagg gttacatcaa acggaaactc
     7501 gttatcaaac caaaggaatc acgtggtggg cagattacaa tgcacttttt gaagagaaag
     7561 gagatccgaa ttcaggaaca ttagaactga attcttgggt cactgttatg aacaaaacgg
     7621 ggctgaattt tgatagcgcg gatcttaaat tagtcgccgg agatgtgaat actgccccgc
     7681 cccagcagct acccagagcg aatcttatga tggctgctaa agtgggatat agtggatctg
     7741 caggctttac agaaaagcct ttttttgaat atcatgttta tgcgctcaat ggtaagatta
     7801 gccttgcaaa taatgccaca caacaaatta aatttttgcc aaaaatctcg aaaataccgg
     7861 ttgaaaagct ttatatttat aatagcaatc cggggatgtt atacacggga tataacaata
     7921 cagaaagata tgttaatacc agcgtgcctg cgaaggtgga tgtttattta aaatggaaaa
     7981 ataccagtag agcagggctg ggaataccgt tgcccgtggg tcgtgttcgg atctctgcca
     8041 tcagcaaatc aaataagagt ccagaattta ttggggaaga tgttattgat catactgctc
     8101 gcgatgaaga aatccaaatt aaaatgggta gcgcttttga tgtgtctgta gaaaaaaaac
     8161 aaacggattt
//
LOCUS       DAORVT010000009         8055 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_5140_length_8055_cov_3.859250, whole genome shotgun sequence.
ACCESSION   DAORVT010000009 DAORVT010000000
VERSION     DAORVT010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8055
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5140_length_8055_cov_3.859250"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="PK389_00405"
     CDS             <1..792
                     /locus_tag="PK389_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="involved in the sodium dependent uptake of proline;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proline symporter"
                     /protein_id="HQY22204.1"
                     /translation="LSLTVGLSFWKMVSLASWGLGYFGMPHILVRFMAVKSVKEIPLA
                     RRVCISWMALSLVGAVLVGIVGAIHFCDCPLGNKETVFMDLSERIFSPWIMGIILAAI
                     LSSSMCAIDSQMLAASSALTEDFYRALFRRKASQRELMWVGRITVIFIAGFAIYLARQ
                     PTQSIMDLVEFAWAGLGATFSASVVLSLYWKRTTRNGIVAGIIVGSMTAIIWRYLGLS
                     GMMNEILPGVILSAVAIYVMSLFDQKPSIEISKEFDTVIKMAKNL"
     gene            792..1367
                     /locus_tag="PK389_00410"
     CDS             792..1367
                     /locus_tag="PK389_00410"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HQY22205.1"
                     /translation="MSAVFIEELDTFIKTSQLYLLPWLYCFAGLWGFNIVNWVCGSPL
                     NIFGIYPRHLFGLLGIIFSPILHKNFNHLFFNSIPLFALGLAILARGLPIFLEVTSVV
                     VVLGGLGVWLFARCALHIGASGLVSGYFGYILATAYFDPSAMALLLAIVVAYYFGSIF
                     LGIFPQEDKISWESHLFGFLAGVASAFHLIN"
     gene            complement(1360..2121)
                     /locus_tag="PK389_00415"
     CDS             complement(1360..2121)
                     /locus_tag="PK389_00415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HQY22206.1"
                     /translation="MLKNLGIIITVSAALLLVGCQSKDKDNYKGMSASEIYAQAEKNV
                     AKEKFPAAAKDFEALEARFPYGEYSDKAQLGLINAYYKQNEPALAISAADRFIRMNPH
                     HPCVDYAYYLKGLVTFEQNYSFTFRYLPLDKSARDPSTATASFDAFKELIDRFPNSKY
                     APDARQRMVFLKNQLANHELSVLKYYVKRGAWLSATNRANYIVKHFEKTSVIPEALAI
                     MVTCYRQLGMQQLANDVLKTLRTNFPDCKELQELN"
     gene            2275..3231
                     /gene="rluD"
                     /locus_tag="PK389_00420"
     CDS             2275..3231
                     /gene="rluD"
                     /locus_tag="PK389_00420"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003150035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HQY22207.1"
                     /translation="MSDLTEHIRLSAPISTELAGKRLDQALALLFPAHSRSRLKDWVD
                     SEKVTVNGVFKRPRTKVVEGDQIEVDAAIEVLSDWVGEPIALDIVYEDTHLLVINKPA
                     NLVVHPAVGNWVGTLVNALLHHCPALSQIPRAGVVHRLDKDTTGLMVVAKTLVAQTSL
                     VNQLQARTVKRIYEAVVYGLILSGNTIKEPIGRHPHDRIRMAVLESGKTAVTHYRVIE
                     RFKSHTHLRVQLETGRTHQIRVHLAHIHHPIIGDKIYGGRLRLPPKSSVELQTCLKDF
                     PRQALHARSLSLLHPETGVDMHWEVSLPADIQQLLSTLRSVS"
     gene            complement(3258..3602)
                     /locus_tag="PK389_00425"
     CDS             complement(3258..3602)
                     /locus_tag="PK389_00425"
                     /inference="COORDINATES: protein motif:HMM:NF024061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HQY22208.1"
                     /translation="MKPTKPEFEEGKSYKPLTKKFLRKIENFTCTNCNTSVTGNGYTN
                     HCPECLYSKHVDINPGDRLAECGGLMAPVAYFKKNGKESLQHKCTKCGLIKNNKISIN
                     DKITNLTVNLLF"
     gene            3790..4809
                     /locus_tag="PK389_00430"
     CDS             3790..4809
                     /locus_tag="PK389_00430"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQY22209.1"
                     /translation="MLVSFSFSSIPSRILNFLFCLSAFLGCCEPIQAAELSASLKPLQ
                     IGILIPMEHAALRDIVAGFETIVRAHYPNRSIYFNVQSAQGDIKLQRSIIELFVGQQV
                     DMIVPVGTSATQMTLALVKELPIVSLAAQYTEVDRQKRAFKNMTGVLDEISGKKKLAF
                     IKAIMPNIKTITLIYHSGNEKNYAEIAELKTESKVLGITLQTLMIQVLPELETAARTI
                     LPDTEIILIGKDHLLASGIRMLVPIAAKRGIPLMTSDEGTVAEGAAVALGVSERMIGE
                     EGGKLATRVLDGYPIKDLPMQEMQALAVFYNPAAQQQKITVDALQDYARKNHVVLMKC
                     EKLMK"
     gene            4916..6955
                     /gene="pheT"
                     /locus_tag="PK389_00435"
     CDS             4916..6955
                     /gene="pheT"
                     /locus_tag="PK389_00435"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HQY22210.1"
                     /translation="MKVSELWLKTLIDLSLSAAALAEQLTNAGIEVDSLDFDAATQQN
                     VFTLKIPPNRGDCLSIEGIARELSLLNAISYQAIAVPSITQGHSETFRICIDAPALCP
                     RYMGCIVKNIKNTAQTPDWLKERLVLGGIRSLSPVVDILNYVMLELGQPMHAFDLATL
                     ESKLVVRKSIAGERITLLDEREIVLDADTLVVADKSKVQALAGIMGGRYSSVTENTTA
                     VFIECAYFDPIAIRLASSRYGIKTDGAYRFERGVDPFLQQRALARSLQLLQEIAGGSI
                     GPVFEQKNDEALPKQSMIFLKTDQISRLLGMSFPADEVVSILKQGGFQVTLEKEGFKV
                     IVPSFRQDISIPVDLIEELARVHGLHRLPSANLTGALNYAAGGARKMPVERLKALLVD
                     SGYYEAITYSFLDLKTFELFEPDSEALVLTNPISAEMAAMRVSLLPGLIEALATNQRR
                     QVMRTRFFEMGVCFFKAEEKTVEKLMLGGVISGTKYAEQWGIAEHPQDFFDIKRDVEA
                     LLALVGKETILFERAAHPAMHPGQTARIFRNGQPIGLVGVLHPRIMKAFSLEGSISVF
                     ELEMQGLMESALPTFVPFSKFPAIRRDIAILVDKSLYAVEPKSSIEQSAGEFLQSVFL
                     FDVYEGKGIPAGKKSVALGLILQHPSRTLIEAEGNDIVKAVVASLQERFDAILRN"
     gene            6959..7249
                     /locus_tag="PK389_00440"
     CDS             6959..7249
                     /locus_tag="PK389_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HQY22211.1"
                     /translation="MTLTKAEIAEGLQEKFRLTKSVAKEIVEQVFEEMRLSLEAGRSI
                     KLSGLGNFDLRDKKARPGRNPKTGEPKVIAARRVVTFKAGQKLKAKIEKIEK"
     gene            7463..7678
                     /locus_tag="PK389_00445"
     CDS             7463..7678
                     /locus_tag="PK389_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22212.1"
                     /translation="MSVQLLRDEDVTQVVGGLLDASATLVTNVPSVPIADGGGDVSSP
                     VTFLGGKPVTVETPKGLLVIPIRAATI"
     gene            complement(7702..>8055)
                     /locus_tag="PK389_00450"
     CDS             complement(7702..>8055)
                     /locus_tag="PK389_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019496905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQY22213.1"
                     /translation="ISAMPMGMHTWVSEGAKNISVGQRQRIILARAIIHKPKILLLDE
                     ATSALDNITQALILTHLAQLKMTRIVIAHRLSTVKNADCIYVLEKGKIVETGTYTHLM
                     KKPGLFEKLVKRQIL"
BASE COUNT         2229 a         1439 c         1877 g         2510 t
ORIGIN      
        1 ttaagtttaa ccgtcggttt atcgttttgg aaaatggtga gtcttgcgtc ttggggactt
       61 ggttattttg gcatgcctca tattctagtt cgatttatgg cggtaaagtc ggttaaagaa
      121 atcccattgg cgcgtcgcgt ctgtatttca tggatggcat tatctttagt gggagcggta
      181 ttagtgggta ttgtcggcgc aattcatttt tgcgattgcc ctttagggaa taaagaaact
      241 gtttttatgg atttatccga acgtatattt tcgccttgga ttatgggtat tattttagcc
      301 gctattcttt cgtctagtat gtgtgcgatt gattcacaaa tgttagcggc ctctagtgca
      361 ttaacagaag atttttatcg cgcattgttt agacgtaaag catcgcagcg cgaattaatg
      421 tgggtagggc gcattactgt tatatttatt gcaggttttg caatttatct ggccagacag
      481 ccaacgcaga gtattatgga tttggtcgaa tttgcgtggg caggattagg tgcaaccttt
      541 agtgcatcgg ttgtattgtc gttatattgg aagcgtacca cccgaaatgg tattgttgcg
      601 ggtatcattg tcgggagcat gacggcaatt atttggcgat acttggggtt gagcggtatg
      661 atgaacgaaa tcttgcccgg cgttattctg tcggccgttg cgatttatgt aatgagctta
      721 tttgaccaaa aacccagcat cgagattagc aaagaatttg atacggttat taagatggcg
      781 aagaatctat aatgtctgca gttttcatag aagagctgga tacttttata aaaacgagtc
      841 aattgtatct tcttccgtgg ctttactgtt ttgcaggctt gtggggcttt aatattgtaa
      901 attgggtatg cggatcgccg ctcaatatat ttgggattta tccgcgacat ctttttggat
      961 tattaggtat tattttttca ccgattctac ataaaaattt taatcatttg ttttttaata
     1021 gcattccgct atttgcattg ggattagcaa ttttagcaag aggcttgccc atttttctag
     1081 aggtgacgag cgtggtcgtt gtcttgggtg gtttaggggt atggttattt gcgcgttgtg
     1141 cattgcatat cggcgcgagt ggactcgttt caggttattt tggttatatt ttggcaacgg
     1201 cttattttga tcccagtgct atggcattgc ttttggcgat tgtggttgcc tattattttg
     1261 gatccatttt tttagggatc tttccgcaag aagataaaat ttcatgggag agtcatttgt
     1321 ttggtttttt agcaggcgtc gccagcgcct ttcacttaat taattaagtt cttgcaattc
     1381 tttacagtcg gggaaatttg ttcgtagcgt tttcaggaca tcattagcaa gctgttgcat
     1441 gccgagctgt cgatagcatg taaccatgat tgcgagtgcc tcaggaataa cagacgtttt
     1501 ttcaaaatgt tttacaatgt aattggcacg attggttgcc gataaccaag cgccgcgttt
     1561 cacatagtat ttgagaacac tcaattcatg attcgctaat tggtttttta aaaataccat
     1621 acgttgacgc gcatcgggtg catacttgct atttggaaag cgatcgatta attctttaaa
     1681 agcatcaaac gatgctgttg ctgttgacgg atcccgagca cttttatcca gcggtaagta
     1741 acggaatgta aacgaatagt tttgttcaaa ggtgacaaga ccctttaaat aataagcata
     1801 atctacacat ggatgatgtg gattcatacg aataaatcga tcagccgcag aaatggctaa
     1861 agccggctca ttttgtttgt aataagcgtt gattaatccg agctgtgctt tatcggaata
     1921 ttcaccataa gggaaacgag cctctagtgc ctcgaagtct ttggctgctg ctggaaattt
     1981 ttctttcgca acgttttttt ccgcttgcgc ataaatctct gaagcagaca tgcccttgta
     2041 gttatcttta tctttggatt ggcagccaac caataaaagg gctgcactaa cagttataat
     2101 aatacctaag ttttttaaca agaaatcacc caaacctgat caatttacca atgccttatt
     2161 ctaacgcagc ttgggtcttg ctgcaattgg gttattccaa gtaaccgctt gtcagcagca
     2221 ttcaagcttg gtcttgcgtt cttagcggtt atccgataag ctggctttca tactatgagc
     2281 gacttaacag aacatattag actttctgcg ccgatttcga cggaattggc tggaaaacgg
     2341 ttggatcaag ccctggcttt gttgtttcca gcacattcgc gatctcgctt aaaagactgg
     2401 gtagatagtg aaaaagtgac tgtcaacggg gtttttaaac gcccgcgcac caaagtggtc
     2461 gagggcgacc agattgaggt ggatgcagca atagaggttt tgtcggattg ggtgggcgag
     2521 cccattgcgc tcgatattgt ttacgaggat acgcatttat tagtgattaa caagcctgcc
     2581 aacttagttg tacaccctgc cgttgggaat tgggtgggta ccttagttaa cgcgctctta
     2641 catcattgcc ctgctttaag ccaaattcca cgtgctgggg tggtacaccg attagacaaa
     2701 gatacgacag gtcttatggt tgttgcgaaa acgcttgttg cacaaaccag cctggtgaat
     2761 caattgcaag cacgaacagt taagcgcatt tatgaagctg tggtctacgg gttgatatta
     2821 tcggggaata ccattaaaga gcctattggg cggcacccac acgatagaat acgcatggca
     2881 gtgttagaat cgggcaaaac tgcggttacc cattatcgtg ttatcgaaag attcaaatcg
     2941 catacgcatt tgcgtgttca gctagaaaca gggcgcacgc atcaaattcg agtgcatcta
     3001 gcacatattc accatccgat tattggggat aaaatatacg gtgggcgttt acggttacct
     3061 cctaaatctt cggtagaatt gcaaacctgt ttaaaagatt ttccaagaca agcactacat
     3121 gccagaagtc tatcgctatt acatcctgaa acaggtgtag atatgcactg ggaagtttca
     3181 ttgccggcag atatacagca attgctgtct acactacgtt cggtttctta gggagaagct
     3241 tatccctgaa cggtatgcta aaaaagcaag tttaccgtta ggttagtgat tttatcattt
     3301 atagaaatct tgttattttt tattaaccca cattttgtac atttatgctg tagagattct
     3361 ttaccgtttt tcttaaagta agcaacagga gccattagac ctccgcattc tgctaagcga
     3421 tctccagggt ttatgtcgac atgcttacta tacaaacatt caggacaatg atttgtatac
     3481 ccatttcccg taacacttgt attgcagttt gtacaagtaa aattttcaat tttccttagg
     3541 aatttcttgg ttagcggctt gtaagatttt ccttcctcga actcaggttt tgttggtttc
     3601 ataataacct atcctctaaa ttgcggatga ccgcatgtct cttgacaaga ctttcttgcc
     3661 tatgttacca tcagaggact atggtatcca agagcggttt ttttaaagca aaatttggtt
     3721 ggcgttggta aaaccagcga cgtctttttt tctgattttg ttctaaatca atgctcagag
     3781 ggtatcaata tgttagtatc gtttagcttc tcctcaatcc catctcgtat tctaaatttc
     3841 ttattttgct taagcgcatt tttaggttgt tgcgaaccca ttcaggctgc agagcttagt
     3901 gctagtttaa aaccactcca gattggaatt ttaattccca tggagcatgc ggcgcttcgc
     3961 gacattgttg cgggatttga gacgattgta cgcgcacatt atcccaatag aagtatttat
     4021 tttaatgtgc aaagcgcgca aggtgatatc aaattacaaa gaagcattat tgaattattt
     4081 gtggggcagc aagtagacat gattgtgcca gttggaacga gtgcaacgca aatgacactt
     4141 gctttggtaa aagaactgcc gattgttagt ttggcagcgc aatataccga agtagatcgg
     4201 caaaaacgtg catttaaaaa tatgacgggt gttctcgatg aaattagcgg caaaaagaaa
     4261 ttggcattta ttaaagcaat tatgccgaat attaaaacaa tcacattgat ttatcacagt
     4321 ggcaatgaaa aaaattatgc ggaaatcgca gaattgaaga ctgaaagcaa agtgttaggc
     4381 attacattgc agacactgat gattcaagtc cttccggagc tagaaactgc ggcacgcaca
     4441 atcctgcctg atacagaaat cattttgatt ggcaaagatc atttattggc cagtgggatc
     4501 cgcatgttgg tgcccattgc cgccaaacgg ggtattccgt taatgacatc cgatgaagga
     4561 acggtagcag agggtgcggc agttgcgctg ggcgttagtg aacgcatgat tggcgaagaa
     4621 ggtggaaaat tggccaccag ggtgttagat gggtacccta ttaaagattt gcctatgcaa
     4681 gaaatgcaag cgttagctgt attttataat cccgctgcgc aacagcaaaa aataacggtt
     4741 gatgcattgc aggattatgc aaggaaaaat catgtagtat taatgaagtg tgagaagctc
     4801 atgaagtaat cttgaggtca tcttgaggtt atcatatgat tacagtctgt ttccaaaaaa
     4861 taaaagttag atttatggta ggatggtgct gataaacacg tggatttcta tataaatgaa
     4921 agttagcgaa ctttggttaa aaaccttgat tgatctttct ctctctgcgg cagcattagc
     4981 ggagcagtta accaatgcgg gcatagaagt ggatagctta gattttgatg ctgccacaca
     5041 gcaaaatgtt ttcacactga agattccgcc caatcgtggc gactgtttga gcatagaggg
     5101 gatagcacgg gagctctcgt tattaaatgc gatttcttat caggccattg ctgtgcccag
     5161 tatcacgcaa ggccattcag aaacttttcg tatttgtatc gatgcgccgg cgctttgtcc
     5221 gcgatatatg ggatgtattg taaaaaatat aaaaaataca gcacagacgc cagattggtt
     5281 aaaagaacgt ttggtattgg gtggcattcg aagtctttct ccagtggtgg atatcctgaa
     5341 ttacgtgatg ttggaattgg ggcagccgat gcacgccttt gatctagcaa cactagaatc
     5401 aaagctggtg gttcgaaaat ccattgcggg tgaacgcata acgcttttag atgagcgaga
     5461 aattgtatta gatgcagata cattggtagt tgcagataaa tcgaaggtac aagcgcttgc
     5521 gggcattatg ggaggacgct attcttccgt aacagaaaat acaacggcag tatttattga
     5581 atgcgcctat tttgatccga tcgcgattcg gttggcgagc agtcgctatg gcataaaaac
     5641 ggatggtgct tatcgtttcg aacgtggtgt tgatcccttc ttgcaacagc gcgcattggc
     5701 acgttctctt caattgttgc aggagattgc aggcggtagt attggtcctg ttttcgaaca
     5761 aaaaaatgat gaggcactgc cgaaacaatc gatgattttt ttaaaaacag atcagatatc
     5821 tcgactttta ggcatgtcgt ttccagcaga tgaagtcgtt tctattttga aacagggtgg
     5881 ttttcaagta acgctagaaa aagaaggttt taaagtgatc gttccgagct ttagacaaga
     5941 tatttctata cctgtcgatt tgattgaaga gcttgcacga gtgcatggtc tccatcgatt
     6001 gcccagtgca aatctaacgg gtgcgttgaa ttatgctgca ggtggtgcac gtaaaatgcc
     6061 ggtcgaacga ttgaaagccc tattggttga tagcggttat tatgaagcca taacctatag
     6121 ttttttggat ttaaaaacct ttgaactttt tgaaccggat tctgaagctt tagttttgac
     6181 aaatcccatt tcagctgaga tggctgctat gcgagttagt ctgttgccag ggttgataga
     6241 agccttagcg acaaatcaac gcagacaagt gatgcgcacg cggttttttg aaatgggcgt
     6301 ttgctttttc aaggccgagg agaaaacagt agaaaagctc atgttaggcg gggttatatc
     6361 gggcacaaaa tatgcggaac aatggggaat agcggaacat ccacaagatt tttttgatat
     6421 aaaaagggat gtagaggcgt tacttgcgtt agtggggaaa gaaacaattc tttttgaaag
     6481 agcagcacac cctgctatgc atcctgggca aacggctaga atttttcgaa atggacaacc
     6541 cattggactt gtgggggtat tacatcctcg tattatgaag gccttctcat tagaaggctc
     6601 tatatcggta tttgaattag agatgcaggg actgatggag tctgcattgc ccacttttgt
     6661 accgttttcg aaatttccgg ccattcgccg tgatattgcc atattggtag ataaatcgct
     6721 ttacgctgtc gagcctaaat ctagtatcga gcaatctgcg ggtgaatttt tacaaagcgt
     6781 ctttttattt gatgtttatg aaggtaaagg gatacctgct ggtaaaaaaa gtgtggcttt
     6841 aggcttgatt ttacagcatc cttcgcgcac gctaatagaa gcggaaggaa atgatattgt
     6901 gaaagccgtt gtggcttcat tacaagaacg atttgatgca atcttaagga attagaacat
     6961 gacgcttacc aaagccgaaa ttgcagaggg tttacaagaa aaatttaggt taacaaaatc
     7021 ggttgctaaa gaaattgttg agcaggtctt tgaagagatg cgtttaagct tagaggcagg
     7081 tagatcgatt aagctttctg gtcttggcaa ttttgacttg cgtgataaaa aagcgaggcc
     7141 gggtcgcaat cctaaaactg gagaacccaa agttattgca gccaggcgtg ttgtaacatt
     7201 taaggctgga caaaaactaa aagcaaaaat agaaaaaata gaaaaatagc aacaacctaa
     7261 acctaaaaaa caatatagct attaagttat acagtagtgc ttgcactaaa cttatgctga
     7321 tgcaacacat aggtaatgct gttttctatt tgtcaaattt ttattttctg tcgaaaggta
     7381 aaaaaaataa actttttcta tttatttttg tctcaattat taggttaaag aatatttaac
     7441 tgaaattaaa taggagagtg tcatgagtgt acaattatta agagatgaag atgtaaccca
     7501 agtagttgga ggattattag atgcgagtgc aacgcttgtc actaatgtac cgagtgtgcc
     7561 aattgctgat ggcggcggtg acgtctcatc tcctgtaacc tttttgggtg gtaagccagt
     7621 tactgttgag acgcctaaag gtttactagt gattcctatt agggcggcta ccatctaacc
     7681 gtgaggtgag atgccagggc tttataagat ttgtcgtttg acaagttttt caaaaagccc
     7741 tggttttttc attaagtgtg tataagtacc cgtttctaca atttttcctt tttctaatac
     7801 ataaatacaa tctgcatttt ttacagtact gagtcgatgt gcaatgacga tgcgtgtcat
     7861 ttttagttgt gcaaggtgtg tgaggattaa agcttgggta atattgtcta aggctgaagt
     7921 tgcttcatct agtaataaaa tttttggttt atgaataatg gcacgagcga gtataattct
     7981 ttgtcgttgt cctaccgaaa tatttttggc accttcggaa acccaagtat gcatacccat
     8041 tggcattgcg ctaat
//
LOCUS       DAORVT010000010         7435 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_5924_length_7435_cov_3.975203, whole genome shotgun sequence.
ACCESSION   DAORVT010000010 DAORVT010000000
VERSION     DAORVT010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7435
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5924_length_7435_cov_3.975203"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PK389_00455"
     CDS             <1..391
                     /locus_tag="PK389_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647174.1"
                     /note="among the AAA+ ATPases, the YifB protease family
                     belongs to the Helix 2 insert clade; unknown function;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent protease"
                     /protein_id="HQY22214.1"
                     /translation="GRISGPLLDRIDLQIEVPAIPQEELMRHNVIGEKSSIIRARVTE
                     SHQRQLNRQGKTNSRLSVKEIDQHCPLDTASENLLKQAISRLNLSARAYHRILKVART
                     IADLSGIEKINNQHIAEAIQYRRLDKQ"
     gene            complement(685..1554)
                     /locus_tag="PK389_00460"
     CDS             complement(685..1554)
                     /locus_tag="PK389_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22215.1"
                     /translation="MKHNFKKYLMVIGATVLLSSCAGYNRILFLTKTNVGIDVSREPL
                     PTAEVTIARREVAISPTYQDTKNGHDKTLPLFGVFSLSDGLFNPNISSVFAGGAAAVA
                     IASDKKMCKDQNNNNDSCDSITLNEYPDDRGFFARLWKEKPKPEETKGQVKPFYFATD
                     TSYGVKVAWDGTGGPYPTSLKIGYNRTEFAYPPILVSQNAGEFIVKIPTFYASIQNKS
                     RFNLNITDPKTGVSQAQVFATGKAAQEWAERPEVQKAALDMIFPPKAAEGEKSANPVE
                     AKKSEDSSDKSGK"
     gene            complement(1583..1939)
                     /locus_tag="PK389_00465"
     CDS             complement(1583..1939)
                     /locus_tag="PK389_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22216.1"
                     /translation="MLIIYVILGTVMSGCAVYYRDRASGAEHIWGIGHLSMKVAAPYE
                     EKQAVIQRSTLTGIAAGMDNGSLGVSVGHDQREHILIYDQNTVITIQRPPSNDFFYFK
                     IGSMPIEVDASANKPK"
     gene            complement(2282..3694)
                     /locus_tag="PK389_00470"
     CDS             complement(2282..3694)
                     /locus_tag="PK389_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HQY22217.1"
                     /translation="MTNKQWSARHLPAKVLNLPKDETAILALFQRESGKTRLCPKSTC
                     LFPTFAQYLTDGFIRTRMPTSNHEDDAVRKQNTSNHEIDLSPLYGRTQTQTMALRCLS
                     EEQGMKGKLKFQKIQGEEYAPFLYKEFSNEYGIKDEFKVLDVPLGINNILNEKEKISR
                     IFAFGGDRANASPQIALMNTLFLREHNRLASKIEETHPDWNDERVFETARNTNIVLFI
                     KIVIEEYINHISPIFKFHVDPSIAWNASWNKPNWITTEFSLLYRWHMLIPDVMRWGKK
                     EYSVENTILNNVPLLDTGLKQSFIDMSAQIAGQIGALNTAQALVMLEENAIKQGRICN
                     LASYNDYRAYVDLPKLVFKDISKNLNVTNLLEKAYHEADDIEFYVGLFSEDVAPNSPL
                     PSLLRTMVAVDAFSQALTNPLLSEHVWPQGQEVFSKAGWEAINQTNSLCDVVNRNCAA
                     QLSGNEFIGMTRQDWRPEWF"
     gene            4334..5647
                     /locus_tag="PK389_00475"
     CDS             4334..5647
                     /locus_tag="PK389_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22218.1"
                     /translation="MTTMISGCVPQYHIKKTEIRPLYSSNAERVDGLRQALSDSPDSP
                     VRIIVVHGMITSEPKYSENLQQRLAEKLGLILSKREEYPTYIYRGYDFIPSLGPQPFD
                     GVIHLKASEVRKSTWVDPSNLNADRLVIYEVLWAPLRDEVKNHFFACFESRSIDPGKE
                     CSTFSKAERNSDYRTIINGALKDSILVNGFADPMIVLGPIGDILRDDITQAICMIASD
                     TLASSGFNLKQSQNMRCDLSSVIQHHKSDQISKQVAATVLEKTKFFAMTHSLGSFLFM
                     DAQQRFAQTRARKNGEISQLSKDEIQESLLFSFTDHATVFMRANQIALLQLGRFSAEG
                     CRPHNNESECPNRLLRVTKKDDTPLGSLTTYVAFNDVNDLLGFELPPYLAATSLFGTL
                     VNVSVQNPGLNVPFIFKNPGAAHLGHEDNPAVIEAMVDGIVFPEK"
     gene            5722..6975
                     /locus_tag="PK389_00480"
     CDS             5722..6975
                     /locus_tag="PK389_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012544926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HQY22219.1"
                     /translation="MTVDNLKILGMPAEKGRWILVMIGLLINVCVGSIYAFSVFRKPL
                     EALWGITSSQSGYPFMVFLAVFAIAMPFAGSLMARWGPKRTTTLGGLLVGTGWILASF
                     SPNITILTILYGVIGGAGVGIVYGCPISVVAKWFPQKSGLAIGLTVMGFGISALLIAP
                     LIKTMIDNPSIGIPNTFLYLGIAFMLAIMLLAQLLSFPPAGWTPAGGIKTAFAATSAE
                     PIPDFSRQAMLKTRDFYALWAIYTIGCLAGLMAIGIASPVGTEVAKLDTTIAALAVSL
                     FAVFNGLGRPIFGALTDKVGPKHAAIVSFTMILGASLLIFFLGQGSATVYLFAFCVLW
                     MCLGGWLAIAPTATGILFGKKHYAQNYGVVFTAYGAGAILGTLLSGNIKDITGSYLGA
                     FPIVAGLAILGIVITVFGFKPVNDK"
BASE COUNT         2226 a         1506 c         1453 g         2250 t
ORIGIN      
        1 cggacgaatc tccggaccat tactcgatcg catcgacctg caaatcgaag taccggcaat
       61 accgcaagaa gagctcatgc gacacaatgt aatcggtgag aaaagcagca taatccgagc
      121 acgcgtgacg gaatcacacc aacgtcaact taaccggcag ggaaaaacca atagccgctt
      181 gagcgtgaaa gaaatcgacc aacattgtcc tctggatacc gccagcgaga acctgttgaa
      241 acaggcaatc agccgtttga atttatccgc gcgcgcgtat catcgcatcc tcaaagttgc
      301 gcgaaccatc gcggatttat cgggtatcga gaaaatcaac aaccaacaca tcgcggaagc
      361 gattcagtat cgcaggttgg ataagcaata aatttcataa taccacggcg acatttctgc
      421 aactgccaga tctgggtcac cattaaataa taattgtccc acgatttgtt gggtccaagt
      481 tgtgccttta attaagatta cattcttagg agctgctcgg cccggtatcg gcggtgataa
      541 gggtaacttt ggttgccgaa cattcagcca aatgagattc aagttacatt gtttgatgaa
      601 gaaggtcccg agatcagtaa aacgctaccc atgtgagtag cgttaccttc cctgacacct
      661 gcacttgaat agataactaa ggatctattt gccgctctta tcagacgaat cttccgactt
      721 cttagcctca actggatttg ccgacttttc tccctcagct gctttaggag gaaatatcat
      781 atctaatgct gccttctgaa cttctggtcg ttcggcccat tcctgagcag cctttcctgt
      841 agcaaaaact tgtgcttgcg agacgccagt ttttggatcg gttatattta ggttaaaacg
      901 gcttttgttt tggatagatg cgtagaaagt tggaattttt actataaatt caccagcatt
      961 ttgcgaaaca agaattggcg gatatgcaaa ttcagttctg ttgtacccaa tcttcaaact
     1021 tgtcggataa ggaccaccgg taccatccca cgctactttt actccataag aagtatcagt
     1081 ggcaaagtaa aaaggcttca cttggccttt cgtttcttct ggctttggtt tttctttcca
     1141 aagccgcgca aaaaaaccgc gatcgtctgg atattcattt aaagttattg agtcacagct
     1201 atcattatta ttattttgat ccttgcacat ttttttatcg cttgcaattg ccacagctgc
     1261 agcaccaccg gcaaataccg acgaaatatt aggattgaat aatccatctg atagagaaaa
     1321 aactccaaat aaaggcaatg ttttgtcgtg gccatttttt gtgtcttgat atgttggcga
     1381 tatcgccact tctctacgag caatggtgac ttctgccgtg ggaagcggtt ctcggctcac
     1441 atcaattccc acattcgttt tagtcaaaaa taaaatccga ttgtatcctg cacaacttga
     1501 taataagacg gttgcaccta ttaccatcag gtattttttg aaattatgct tcattgttaa
     1561 ccctcctgtt ttattagcgc acttactttg gtttgttggc agaagcgtct acttcaattg
     1621 gcatcgagcc aattttgaaa tagaagaaat cattactcgg tgggcgttga atggtaatca
     1681 ccgtattttg atcataaatt aggatatgtt ctcgctgatc atgcccgaca ctcacgccca
     1741 acgaaccatt atccattccc gcagcgattc cggttagcgt agaacgttga atcactgctt
     1801 gcttctcttc atagggcgcg gctactttca tcgagagatg accaatcccc caaatatgct
     1861 cggcaccaga tgcacgatca cggtagtaca ctgcacaacc cgacataact gtacccaata
     1921 tgacataaat aattaacatg agaatgaacc taacagttaa tgttgtaatt ggcatgattc
     1981 taaacttata agaaaaaaat aaacttcttc tttcacacag agaatgacga tacagaatcc
     2041 cagatagagt gtcaaactca ctgacaaaca ataagaaatt cacagaattt gcacttaaca
     2101 gtagcaaaaa gcattagttt taatccttac tttactatta acgcgataga tactgatgaa
     2161 gcgacttgta tgctaaaaaa tgcaaaaggt ccgactcatt gacacggtcg ttaaagagta
     2221 tgaattgtca taacccaaaa gccaacttaa tcagagcctc ctcaagactc attgcaacat
     2281 atcaaaacca ttctggacgc cagtcctgac gagtcattcc aatgaactca ttgccggaca
     2341 attgagcagc acaatttcta ttaacgacat cacacagact atttgtctga ttaatcgctt
     2401 cccaacctgc cttggaaaaa acttcttgcc cttgtggcca gacatgctca gaaagcaggg
     2461 ggttagtcag cgcttgagaa aaagcatcga ctgcaaccat tgttcttaac aaacttggta
     2521 gtggcgaatt aggtgctaca tcttcggaaa ataaacccac ataaaattca atatcatcag
     2581 cctcatgata agctttttcc agaagattag tgacatttag gtttttagaa atatctttga
     2641 aaactaattt tggaagatct acatatgctc gatagtcatt ataagaagct aggttacaaa
     2701 ttcttccttg cttaattgca ttttcctcaa gcatcacaag tgcttgagca gtattaagag
     2761 cacctatttg accagcaatt tgtgcactca tatcaataaa ggactgcttc aaaccagtat
     2821 caagtaatgg tacattgttc aatatagtgt tctcaacaga atattctttc tttccccatc
     2881 tcattacatc tggtataagc atatgccagc gataaagtaa gctgaattca gttgtaatcc
     2941 aattaggttt gttccaagat gcattccatg cgatagatgg gtcaacgtga aatttgaata
     3001 taggggaaat atgattaata tattcttcga taacgatttt tataaaaaga acaatattcg
     3061 tgttccgtgc tgtctcaaaa actctttcat cattccaatc tggatgtgtc tcttcaattt
     3121 tacttgctaa gcgattatgc tcacgaagaa acaaagtatt cattaaagct atttgaggag
     3181 atgcattagc acgatcacct ccgaatgcaa aaatgcgtga aattttttct ttctcattaa
     3241 gaatattatt tatacctagt gggacatcaa gtactttaaa ttcgtcttta attccatatt
     3301 cattgctaaa ttctttatat aaaaaagggg catactcctc accctggatc ttctgaaatt
     3361 taagtttgcc tttcataccc tgctcttcgc ttaaacaacg aagcgccatc gtttgtgttt
     3421 gcgttctacc gtataaagga ctcagatcaa tctcatgatt tgaagtattt tgcttacgta
     3481 cagcatcatc ttcatggtta cttgtaggca tacgcgtgcg gatgaatccg tctgttaaat
     3541 actgagcaaa agttggaaat aagcaggtag attttggaca gagtctggtt ttgccagatt
     3601 ctctctgaaa taaagctaaa atcgccgttt catcctttgg taaattaagc acctttgctg
     3661 gaagatgtct cgcactccat tgtttatttg tcaatgaagt ccaagatacg tagtcatgta
     3721 ctgtgctcca tgggtgaggg cggtgcctgc aaacgttaac agctctatta gtcgcaaatt
     3781 tattaattaa tctccgtaaa aaaggagata tctcagagat tttgttaaca atagttaagg
     3841 caagggtttc aataaatgtt gggagcttta ttcatattcc ttttttaaag atcaagccaa
     3901 tgtttcaaaa tgcaggtcat ttgatacctg gatctagatt ttaaaaatat agcttaattt
     3961 ttaggataat gctttttgaa ttacgaatca gtgagaaaaa tcacacttct cttttagaga
     4021 tctagttttt ttatttctgg aaacactgat ctattcgatc acatcgaata gatttgcaga
     4081 ggtttgataa aatacattga tattgttaaa aatttgatga taaataccac atcaaagata
     4141 ttcaagccgt cgttgagaaa attgatgccg gaaataaaaa ctgtagtatt tgattcaatg
     4201 tcataatcag gcagtttcat attttactgt gagactgctt gactcttata tctatcaacc
     4261 aattgtcttt attgcaacga aatagatcgt gctatgtttt cataagattc tgattacaat
     4321 cttagtggtt gcgatgacaa ctatgatctc aggctgtgta ccacaatatc atattaaaaa
     4381 aactgagatt cgaccgcttt atagttcaaa tgctgagcgt gttgacggcc ttcggcaagc
     4441 actctcagac tccccggata gtccggtacg aatcatagtg gtacatggga tgattaccag
     4501 cgagcctaaa tacagtgaga atttacagca gcgacttgct gaaaaacttg gattgattct
     4561 gagtaaaaga gaagaatatc ctacttatat ttaccgggga tatgatttta ttccttcttt
     4621 gggcccccaa cctttcgatg gagtaattca cctaaaagct tcggaagttc gaaaatcgac
     4681 atgggttgat ccaagcaatt tgaacgctga tcgtttggtg atttatgagg tgctatgggc
     4741 gccgcttcga gatgaggtaa aaaaccattt ttttgcttgt ttcgaatctc gctctattga
     4801 tcctggaaaa gaatgctcaa ctttttccaa agcagaacgc aattcagatt atcgcaccat
     4861 aattaatggc gccctgaaag atagcatact tgtgaatggt ttcgcagatc caatgattgt
     4921 tttaggcccc ataggcgata tccttagaga tgacataact caggcgatct gtatgattgc
     4981 aagtgatacg cttgcttcga gtggatttaa tttaaagcaa tcgcaaaata tgcgatgcga
     5041 tctttcttca gtcattcaac accacaaatc ggatcaaata tcgaaacaag ttgcagcaac
     5101 agtcttagaa aagactaaat tttttgcaat gacacatagt ctcggtagct ttctgttcat
     5161 ggatgcacaa caacgatttg ctcaaacaag agcaagaaaa aatggggaaa tttcgcaact
     5221 aagtaaagat gaaattcaag aatctttatt gttttccttt actgaccacg ctaccgtttt
     5281 tatgagagca aatcaaatag cattgcttca gctcggaaga ttctctgcag aaggctgtag
     5341 accgcacaac aacgaatctg agtgccctaa tcggctcctt cgggtgacga aaaaggatga
     5401 tacgcctctt ggttcactga caacttatgt tgcattcaat gacgtcaatg atcttttagg
     5461 ttttgagcta ccaccctatc tggccgctac gagtctattc ggaacacttg taaacgtttc
     5521 agtacaaaac ccaggactaa acgttccatt tattttcaag aatcctggcg ctgctcattt
     5581 aggacatgaa gataatccag ctgttataga agcaatggta gacgggatag tattcccaga
     5641 aaagtaaagt caaaaaatat agctcaaatc gaatctgata aaaacctaaa aaacgcattg
     5701 gacaagggct aaactattta gatgactgtg gataatttga aaatattagg catgccagct
     5761 gaaaaaggca gatggatatt ggttatgatc ggattgttga tcaatgtctg tgttggatcc
     5821 atttatgcat tcagcgtttt caggaaacct ctggaggcac tatggggaat aacttccagc
     5881 caaagcggtt acccatttat ggttttcctt gcagtatttg caattgccat gccatttgca
     5941 ggcagcttaa tggcaaggtg gggtccaaaa agaacaacta cgcttggagg cttattggta
     6001 ggaacaggat ggattttggc cagtttttct ccgaatatta ctatcctgac cattttatac
     6061 ggcgtaatag gtggcgctgg cgtcggaata gtttacggtt gtccgatttc agtagtagca
     6121 aaatggttcc ctcaaaaaag tggtctagcc attggtttaa cggttatggg ttttggcata
     6181 tctgcattgt taatcgcgcc actcataaaa acaatgatag ataatccgag tattggaata
     6241 ccgaatacat ttctttactt agggatagca tttatgttgg caatcatgct tttagcccaa
     6301 ttattaagct tcccacctgc cggatggacg cccgcaggag ggataaaaac agcttttgct
     6361 gcaacttctg cagaaccaat tcctgacttt agcagacaag caatgctaaa gactcgcgat
     6421 ttctatgctt tgtgggctat ttacactatc ggatgtttgg cgggactaat ggctattgga
     6481 atagcatccc ctgttggtac agaagtggcc aaattggata cgacaatagc cgcgcttgct
     6541 gtttcattgt ttgctgtatt taacggtttg ggccgaccaa tatttggagc gcttactgat
     6601 aaggtgggcc caaagcatgc agcaatcgtt tcatttacca tgatactggg agcatcttta
     6661 ctgatattct ttctaggaca aggcagcgca acggtgtacc tatttgcatt ctgcgtttta
     6721 tggatgtgcc tgggtggatg gcttgcaatt gcaccgaccg caaccggaat tctttttgga
     6781 aagaaacatt atgcgcagaa ttatggagta gtgtttactg cctatggtgc aggtgcgatc
     6841 ctaggcacac tgctatccgg taatatcaag gatatcaccg gaagctattt gggtgcattt
     6901 ccaatcgttg cgggcctagc aattctcgga atagttatta ctgtttttgg atttaaaccg
     6961 gtaaacgata aataatcgag ctaaaaagat taacacaatc atatgttcgc cttcatttag
     7021 tatcagatcc aaatttaatt cgtcaattgt gctttttatt gacgataaat gcagatccca
     7081 tgcgctcctc accttccgtt ccaaccaaaa atcttgattt aagaactcag atttacaatc
     7141 caaatttgct acttggtgtc aattatttac atcagttact tcatgctaat gtcagacttg
     7201 actgctgtta ttctgctatt cattatgtaa gttttcctac agaaaattta aaccaattca
     7261 attaatctac ataattccta tgttaatggc atggaataag atcatgcaga tttataattt
     7321 gttttctggg aaaccagaca ctttttacag tgctctaatc gactattatg atagttaaaa
     7381 accgatacta ttatcgttcc aggatcaaag aagtgaaatt caggcagatt attcg
//
LOCUS       DAORVT010000011         7262 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_6178_length_7262_cov_3.728181, whole genome shotgun sequence.
ACCESSION   DAORVT010000011 DAORVT010000000
VERSION     DAORVT010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7262
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6178_length_7262_cov_3.728181"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..833
                     /gene="bioD"
                     /locus_tag="PK389_00485"
     CDS             126..833
                     /gene="bioD"
                     /locus_tag="PK389_00485"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HQY22220.1"
                     /translation="MKKNIKGFFVTGTDTDIGKTLIASALLILLKQKHFKTVALKPIA
                     SGCIMTSKGLRSPDAYLLQKYMTEKLAYSEINPFAFLEPISPHIAAKIADQVLSVKII
                     MDACQDILKKQIDYLVIEGIGGWLVPLNAHETTADLAKAFGYPIILVVGMRVGCLNHA
                     LLTIESMRKKSITLAGWVANSMQSEIPYLTDHIETLQRSIPAPLLGIVPHLTHVEPFN
                     PSLAVPYLKQMYHLISF"
     gene            1031..1828
                     /locus_tag="PK389_00490"
     CDS             1031..1828
                     /locus_tag="PK389_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HQY22221.1"
                     /translation="MYQETLELVAESAKNKAKLCSSNLLAYLMHAAMAGIYLGFGVVL
                     VFSLTTSFFQAESPGLSLVMGTTFGIALSLVVIAGADLFTGNTMIMLIGALKGKTTWT
                     DLSKIWFWSYCGNFLGAIIFALIAWKADVLQDASWLLTVATKKMNGSWMSLFLKGILC
                     NWLVVLAVWCSFKLKTEGAKLIMIWWCLLGFVGSGYEHSIANMTLLTLANLLPNDGIH
                     IVSWSGVIHNLIPVTLGNIVSGVLFMGIPYYFISYPESVPKQDLRNY"
     gene            complement(1971..3356)
                     /locus_tag="PK389_00495"
     CDS             complement(1971..3356)
                     /locus_tag="PK389_00495"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HQY22222.1"
                     /translation="MKTYLFYDIETTGLNKAFDQILHFAAIRTDLNFNELKRYELIVK
                     LNPDVIPSPEAMLVHRMKLSDVANGISEYEAIQKIHAWMNEPGTISLGYNTLGFDDEF
                     LRFSFYRNLLPPYTHQYANQCCRMDIYPIVVLYYLFKNSAIAWPEVHGSVSLKLEDIN
                     NANKFVTGRSHHAMVDVESTIALSKQLIKEREMWDYVCGNFDKKIEQVRLKKINTESA
                     LMVLGKFGFKQKYQSQVLPLGEHVHYKNQILFLRLDDKNFTAATKENILESTFVVRKK
                     LGEAGFLFPFTERYTYCLDAERLALANVNKQWLKDNEALLTFVSDYHRHYEYPAVPDI
                     DVDASLYVNGFWTSEEAMFCREFHSAAPDKKSKMIETVQNPNLSALAIRILGRHFPEC
                     LNSGQAEYFKNYIAQINNNATAPIIDYKGAARLTPKIALQKIAEARNENSLDCEQIGL
                     LNNLEFRLHAT"
     gene            3569..4894
                     /gene="miaB"
                     /locus_tag="PK389_00500"
     CDS             3569..4894
                     /gene="miaB"
                     /locus_tag="PK389_00500"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018946621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HQY22223.1"
                     /translation="MTPKTVYIKTYGCQMNVYDSTRILDLLKATHGITGVDNPEEADL
                     LLINTCSIREKPHETVFSELGVWGAFKEKNPEKVIGVGGCVASLEGEAILKRAPYVDL
                     VFGPQTLHRLPNLLSAVTKTRSPQVDVSFPLIEKFDHLPNPRADGPTAFVSIMEGCSR
                     YCTYCVVPYTRGEEISRTFDDVLHEVYVLAEQGVREVTLLGQNVNAYRGKMQSGQIAD
                     LALLISYISEIDNIARIRFTTSHPLEFSDRLIQAYADNPKLANHLHLPVQSGSDRILQ
                     LMKRGHTAEQYKTKIQKLREVRPNISITSDFIVGFPSETEDDFNATLNLVEALQFDQS
                     FSFIYSQRPGTPAANIPDNISNIIKKQRLAILQARLAEQAAELSQNMVGTTEMVLVTG
                     PSKKDPSILCGRTDNNRVVNFEGKVPVGSLVPILITEVVTNTLRGKWVA"
     gene            4961..5968
                     /locus_tag="PK389_00505"
     CDS             4961..5968
                     /locus_tag="PK389_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HQY22224.1"
                     /translation="MSDSSVQVFDITLTPVDNRRLANLCGRFDEHLRQIEHRLRIEIT
                     NRGNTFQLIGPPPAIHIATQVLKALYEETKHSIELSPSKVHLALQQYARTDTTLETEG
                     QAAISTLNTRAGEVRPRSPNQTQYIKNILSHDINFGIGPAGTGKTFLAVASAVAAFEQ
                     EKIRRILLVRPAVEAGERLGFLPGDIAQKIDPYLRPLYDALYEMLGFERVAKLLERQV
                     IEIASLAYMRGRTLNDAFIILDEAQNTTVEQMKMFLTRLGFSSTAVITGDITQIDLPK
                     GKLSGLKQVMGMLQSTPGISFTFFNSSDVVRHPVVQKIIEAYALYDTKHNNAKGNTGE
                     Y"
     gene            5958..6422
                     /gene="ybeY"
                     /locus_tag="PK389_00510"
     CDS             5958..6422
                     /gene="ybeY"
                     /locus_tag="PK389_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011866722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HQY22225.1"
                     /translation="MSIELELQIATLAKTLPHPAQIREWVNLTLYNRVETAELTIRIV
                     DEAESADLNQQYRNKPGPTNVLSFPYDPEPGIATRLLGDIVICAPIVEQEAILENKSL
                     LAHWAHMVIHGLLHLLGYTHDFDSDAAEMENLETELLIRLNFPPPYGDVIVS"
     gene            6419..>7262
                     /locus_tag="PK389_00515"
     CDS             6419..>7262
                     /locus_tag="PK389_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006913181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HQY22226.1"
                     /translation="MSDESQPSQSPTRTWLEKIGQILAGEPRDRNELIEILRDATERR
                     LLDLNALGMIEGILQVPELRVRDIMIPRSHIVVIEQDMLFDQILPIVITTVHSRFPVI
                     GENKDEVLGILLAKDLLSYTFDSTTNKPFNIREVLRPAVFVPESKRLDVLLQEFRRNR
                     NHMAVVVDEYGGVTGLITIEDVLEQIVGDIEDEYDTDDNQLIKKYHENTYAVKALTTI
                     DDFNEFFHTNIDDKDADTIGGLVTREFGHLPKRGESVSVNGYKFKVLRADSRRIHLLQ
                     VMQIK"
BASE COUNT         2247 a         1446 c         1378 g         2191 t
ORIGIN      
        1 aaaagtcttt tatcaaaaaa aaaattatac ggtgtttttg aagagtatga aaaattcagg
       61 aacaaacaag atatgttacc tgcaaggtac gaaacttatt tcatcttagc aacaaaggca
      121 ccttaatgaa gaaaaatatc aaaggttttt ttgttacggg aacagataca gatattggaa
      181 aaacactgat tgcttctgcc ctacttattt tgctaaaaca aaaacacttt aaaaccgtgg
      241 cactaaagcc aatcgcttct ggatgtataa tgacctctaa aggattgcgt agtcctgatg
      301 cctatttatt acaaaaatac atgactgaaa aattagcata ctccgaaatt aatccttttg
      361 ctttcttgga gccaatatca cctcatattg cagcaaaaat tgcagaccaa gtgctatccg
      421 ttaaaatcat tatggatgct tgtcaagata ttttaaaaaa acagatcgat tatttagtca
      481 ttgaaggtat tggtggttgg ctagtccctt taaatgcaca tgagacaaca gcagatttgg
      541 caaaagcgtt tggataccct attatattgg tggttggtat gcgcgtaggt tgccttaatc
      601 acgccctttt aaccatcgag agcatgcgga aaaaatctat aactcttgcc ggttgggtag
      661 ctaattcaat gcaatctgaa ataccctatt taactgatca cattgaaacg ttacaacgat
      721 ctatacctgc tcccttatta gggattgtcc ctcatttgac acatgttgag ccctttaatc
      781 cgtctttggc ggtcccttat ctaaagcaaa tgtaccactt gatttctttt taaaacttta
      841 ttggaaattt gcacctagtg tgcacttgag tagactaggc atacgttaat tagctaacaa
      901 cctgtccgag cgcttaaaat ctaacttttt gatctttaaa ccgtggtatt tcagacatgt
      961 tattatgtta ttatacgtcg ctatcgcatc ggttcgatgt ggataataaa gaaaataaat
     1021 aggaagcatt atgtatcagg aaacattaga attagttgct gaatcggcaa aaaataaagc
     1081 gaaactctgt tcttcaaatt tgcttgctta tcttatgcac gctgctatgg caggcatcta
     1141 tttgggattt ggtgttgtct tagtcttttc attaacaact tcttttttcc aagcagaatc
     1201 tccaggacta agtcttgtta tgggcacaac ttttggcatt gcactttcct tagtcgttat
     1261 tgcaggtgca gatttgttta cgggaaacac catgattatg ctgattggtg cactgaaagg
     1321 taaaacaacc tggactgatc tatcgaaaat atggttttgg tcgtattgcg ggaatttttt
     1381 aggcgctatt atatttgctt taattgcttg gaaagcagat gttttacaag acgccagttg
     1441 gcttttgaca gttgccacaa aaaaaatgaa tggatcttgg atgtcacttt ttttaaaagg
     1501 aattttatgt aattggctcg tggtacttgc tgtatggtgt agttttaaac ttaaaactga
     1561 aggtgcaaaa ctgattatga tttggtggtg cttactcgga tttgttggtt ccggttacga
     1621 gcatagcatc gccaacatga ctttgttaac cttagcaaat ctacttccaa atgacggtat
     1681 tcatatagtt tcttggagtg gagtaataca taatttaata ccagtcacat taggaaatat
     1741 tgttagtggt gttttattta tgggaattcc ctattacttt atcagttatc cagaatctgt
     1801 gcccaaacaa gatttaagaa attattaatg gtttacattt tctgctcttt tagcagcaaa
     1861 aattttatac gcgtgaaaat aaaaaacata tacttttcat agatatttcg catggtttgt
     1921 atgataatgc actctttatc tttaagtcca acaacctcaa tttgtaggtt ctatgtcgcg
     1981 tgcaatctaa actctaaatt attcaataag ccaatttgct cacaatccaa actattttca
     2041 ttccgagctt ctgcaatttt ttgcaatgca atttttggcg ttaaacgtgc agcgcctttg
     2101 taatctatta tcggtgcagt tgcgttattg tttatttgcg ctatataatt tttaaaatac
     2161 tcagcctgtc ctgaatttaa gcattctggg aaatgacgac ccaaaatacg aatagcaagc
     2221 gccgataagt ttggattttg tacggtttca atcatttttg actttttgtc tggtgccgca
     2281 ctgtgaaatt ctctgcaaaa catcgcttct tctgatgtcc aaaaaccatt aacatacaaa
     2341 cttgcatcca catcaatgtc cggcacagcc ggatattcat aatgacggtg ataatctgaa
     2401 acaaaagtaa gcaatgcttc attgtcttta agccattgtt tattgacatt ggcaagcgct
     2461 aaacgttctg catcaagaca gtacgtatag cgctccgtga atggaaataa aaagcctgct
     2521 tcacccagct tttttctaac aacaaaagtt gattctaaaa tattttcttt tgtggccgca
     2581 gtgaaatttt tatcatctaa tcttaaaaat aaaatttgat ttttataatg aacatgctcg
     2641 ccaagaggca aaacctggct ttgatacttt tgcttaaacc caaacttccc tagcaccatt
     2701 aatgcactct ctgtgtttat tttttttaaa cgcacctgtt ctattttttt atcaaaattg
     2761 ccacaaacat aatcccacat ttcacgctct tttataagtt gttttgaaag cgctatagtg
     2821 gattcaacat caaccatcgc atggtgcgaa cgtccagtaa caaacttatt tgcattattg
     2881 atatcctcta gcttaagaga aacacttcca tgaacctcag gccatgcgat cgctgaattt
     2941 ttaaataggt aatacaaaac gacaatagga tagatatcca tgcgacagca ctgatttgca
     3001 tactgatggg tataaggcgg caacaaattt ctataaaaag aaaaacgtaa aaattcatca
     3061 tcaaatccca aggtgttata ccctaaactg atagtcccgg gttcattcat ccaagcgtga
     3121 attttttgta tcgcctcata ttcagaaatg ccattagcaa cgtcgcttaa tttcatccgg
     3181 tgtaccagca tagcttcagg tgacggaata acatctggat taagcttaac gataagctca
     3241 tagcgcttta attcattaaa atttaaatct gttcgaatag cagcgaagtg caatatttgg
     3301 tcaaatgctt tgtttaatcc cgtggtttca atatcgtaaa ataaatatgt tttcaatgtg
     3361 ctgtccctaa atatacctga ctcaataata cacgaatgaa gtgtaaaaac gcatcaatat
     3421 tcaatccgca tattcaataa gaacagattt ttcatgaaat tagttataaa gcgaacattc
     3481 tgtttgcagc ttttcgattg aactcattct gatcctgaag tacaatgcga actgatccta
     3541 aaacaccctt ttaagaatga caccagatat gacgccaaaa acggtttaca ttaaaacata
     3601 tggttgccaa atgaatgtct acgattcaac ccgaatcttg gacttgctga aggcaacaca
     3661 tggcataacg ggtgtagaca accctgaaga agcagatctg ttactgatca atacctgttc
     3721 tattcgtgaa aaaccgcatg agacagtatt ttcggaatta ggtgtttggg gtgcattcaa
     3781 agagaaaaat ccagaaaagg ttattggcgt tggcggttgc gttgctagcc ttgaaggcga
     3841 agccatttta aaacgcgcac cttatgtgga tttagtgttt ggaccacaaa ccttgcaccg
     3901 attacccaat ctgttatcag cggtcactaa aacccgaagt ccgcaggttg atgtctcttt
     3961 tccgctaatt gaaaaatttg atcacttgcc caaccctcgt gcggatggcc ccactgcttt
     4021 tgtttcgatt atggaaggtt gtagcagata ttgcacttac tgtgtcgtgc cttatacgcg
     4081 tggtgaagaa atcagtcgta catttgatga tgtgctacat gaagtttatg tattggcaga
     4141 acaaggcgta cgcgaagtca ctttgctcgg acaaaacgtt aacgcctatc gcggtaaaat
     4201 gcaatctggc caaattgctg atctcgcact gctgatttca tatatctccg aaattgataa
     4261 tatcgcacgt attcgcttta caacatcgca tcctctcgaa ttttctgatc gtttaataca
     4321 ggcatatgcc gataacccaa aacttgcgaa tcatctacat ttgccagtac agagtggatc
     4381 agatcgcatc ttacaactga tgaaacgcgg tcatactgcc gagcaatata aaacaaaaat
     4441 tcaaaaatta cgagaagtac gacccaacat tagtattaca tccgatttta ttgttgggtt
     4501 tcccagcgaa acagaagacg attttaatgc gacactcaat ctggttgaag cattacagtt
     4561 cgatcagtct tttagtttta tttatagcca acgaccggga acaccggcag ccaatattcc
     4621 cgataacatt tcgaacatca ttaaaaaaca acgcttagca atattacaag cgcggcttgc
     4681 cgaacaagca gcagagctca gccaaaacat ggtcggaaca acagagatgg tgttggtgac
     4741 gggtccctct aaaaaagacc cttcaatact gtgtggacgc acagataata atcgggtcgt
     4801 caactttgaa ggtaaggtcc ctgttggaag cctcgtgcca attctgatta cagaagtcgt
     4861 taccaatact ttacgtggaa aatgggtagc ttaactggca attccaggtt atgcttagga
     4921 tacaagatcg gggagacccc gtaaaacggt taaatataat ttgagcgact cttcagtaca
     4981 agtgtttgat attacattaa ctccggttga taaccgccga ttggcgaact tatgcggtcg
     5041 gtttgatgag cacctgcgtc aaattgagca tcgactccgt attgaaatca ccaatcgtgg
     5101 caataccttc caactgatag gaccaccgcc ggcaatccat attgcaacac aggtattaaa
     5161 agcgctttat gaagaaacca aacatagcat agagctttct ccttcaaaag tgcatttggc
     5221 tttacaacaa tacgccagaa cagataccac gctcgaaaca gaaggccagg cagccattag
     5281 caccttaaat acccgcgcgg gtgaagtacg tccccgctct cctaaccaaa cacaatatat
     5341 aaaaaatatt ttaagccatg acatcaattt tggcataggt ccagcaggaa ccggcaaaac
     5401 ttttctcgcg gttgccagtg ctgttgccgc ttttgaacaa gaaaaaattc gtcgcatttt
     5461 gcttgtacgt cctgcggttg aagcaggtga acgtttaggt tttctacctg gtgatatcgc
     5521 acaaaaaatt gatccttact tgagaccctt atacgatgcg ctttatgaaa tgctcggatt
     5581 tgagcgtgtt gcaaaattat tagaacgaca ggtcattgaa attgcttctc tcgcctacat
     5641 gcgtggtcgc acattaaatg atgcttttat tattttagac gaagctcaaa atacaacagt
     5701 agaacaaatg aaaatgtttt taacacgttt gggctttagc tcaactgctg ttataacagg
     5761 agatatcaca caaattgatc tccccaaagg aaaactatcg ggcctaaaac aagttatggg
     5821 aatgctacaa tctacgcctg gcattagttt tacttttttt aattcgagtg atgtcgtcag
     5881 acatcccgtc gtacaaaaaa ttatcgaagc ctacgcgcta tacgatacaa aacataataa
     5941 cgccaaagga aatacgggtg agtattgagc ttgagctgca aattgcaacc cttgccaaaa
     6001 cattaccgca ccccgcacaa attcgtgaat gggtaaatct aacgctatat aatcgtgttg
     6061 aaacagcgga attaacgatt agaattgtag atgaagccga aagcgctgat ctcaatcaac
     6121 aatatcgaaa caaaccagga ccgacaaatg tattatcttt tccttatgat cctgaaccgg
     6181 gtatcgccac tcgtttactc ggcgatatcg ttatttgtgc tccgattgtt gagcaagaag
     6241 ccattctgga aaataaatcg ctcttagcgc actgggcgca tatggtgatc cacggcttat
     6301 tgcatttatt aggatatacc catgattttg acagtgatgc cgccgaaatg gaaaatctag
     6361 aaacagaact tttaattcgg cttaattttc caccccctta cggagatgta attgtttcat
     6421 gagtgacgaa tcacaacctt cacaatcgcc cacccgaact tggttagaaa aaattggtca
     6481 aattctggct ggtgaaccac gcgatcggaa tgaattgatc gaaattttac gagatgccac
     6541 agaaagaaga ttattagatc tgaatgccct cggtatgatt gaaggcattt tacaagtacc
     6601 ggaactgcgt gtgcgcgata ttatgatccc acgttctcat attgtggtta ttgagcaaga
     6661 catgctattt gatcaaatct tacccattgt gattaccaca gtgcattctc ggttccctgt
     6721 tattggcgaa aataaagatg aagttttggg gatcttactc gcgaaagatc tcttgtctta
     6781 tactttcgat tcaaccacaa ataaaccctt taatattcgg gaagtcttgc gtcctgctgt
     6841 gtttgtgccc gaaagtaaac gcctcgatgt attactacaa gaatttcgca gaaatcgaaa
     6901 ccacatggcc gttgtcgtag atgaatatgg tggtgtcaca ggccttatta cgattgaaga
     6961 tgttttagaa caaattgtcg gtgacatcga agatgaatat gacacagatg acaatcaatt
     7021 aattaaaaaa tatcatgaaa atacttacgc tgtaaaagca ctcacaacga tagacgattt
     7081 taacgaattt tttcacacga atatagatga taaagatgcg gataccatcg gcggattggt
     7141 tacacgagaa tttggccatt taccaaaacg cggagagtct gtctccgtca atggttacaa
     7201 atttaaagtg ctaagagccg atagtcggcg cattcatcta ttgcaggtta tgcaaataaa
     7261 ac
//
LOCUS       DAORVT010000012         7258 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_6190_length_7258_cov_4.006942, whole genome shotgun sequence.
ACCESSION   DAORVT010000012 DAORVT010000000
VERSION     DAORVT010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7258
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6190_length_7258_cov_4.006942"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PK389_00520"
     CDS             complement(<1..224)
                     /locus_tag="PK389_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22227.1"
                     /translation="MQIDTIVNRALALDPNAIGQLKKLYGKCLEIFLKDFEKIFFMCF
                     TETGIQFTLTKPEIPEKCDARISGPIKAFF"
     gene            complement(229..975)
                     /gene="ubiE"
                     /locus_tag="PK389_00525"
     CDS             complement(229..975)
                     /gene="ubiE"
                     /locus_tag="PK389_00525"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006034473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HQY22228.1"
                     /translation="MAEETTHFGYQSIATDQKTERVGSVFRSVANRYDLMNDLMSFGL
                     HRFWKRLAIARSNIRPHHTVLDLAGGTGDLTRQVAKLLGKEGLIVLADINEAMLTKGR
                     ERLINAGFIQPIALTQANAESIPFANNSFDRIICAFGLRNVTHPNKALAEMSRVLKPG
                     GQVMILEFSTVNDKMLGTLYNAYSFSILPKLGEWICNDRNSYQYLAESIRVHPNQETL
                     KSMMCEAGFERTEYQNLHAGIVALHTGYKF"
     gene            complement(976..2241)
                     /gene="rho"
                     /locus_tag="PK389_00530"
     CDS             complement(976..2241)
                     /gene="rho"
                     /locus_tag="PK389_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HQY22229.1"
                     /translation="MNLTELRHKSPTELMEIAQTFGIEDVARARKQEVIFAILKAHAK
                     KGEDIYGNGVLELLPDGYGFLRSADSSYLAGPDDIYVSPSQVRRFSLRTGDEIFGKIR
                     PPKDQERFFALLKVDTINFEPPEITRHKIAFENLTPLHANKPLIMQTGDGSTRDITSR
                     VIDLVSPIGKGQRGLIVSPPKAGKTVMLQNIAHSIAVNHPECHLIVLLIDERPEEVTE
                     MSRSVRGEVIASTFDEPASRHVQVASMVIERAKRLVEHKRDVVILLDSITRLARAHNT
                     VIPPSGKVLTGGVDANALHLPKRFFGAARNIEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNLELHLDRRISEKRVYPAINIARSGTRREELLAKPEDLQKMWILRKLLHPMDD
                     LAAMEFLLERLRDTKTNEIFFESMKRAAQ"
     gene            complement(2386..2715)
                     /gene="trxA"
                     /locus_tag="PK389_00535"
     CDS             complement(2386..2715)
                     /gene="trxA"
                     /locus_tag="PK389_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HQY22230.1"
                     /translation="MSTHLQPVTDSSFDTDVLQSTTPVVVDFWAEWCNPCKMIAPLLE
                     EAAVTYAGKIKIVKLNVDENNTTAAKFGIRGIPTLLVFKNGKVAATKVGHLTKSQLSE
                     FLDSHLG"
     gene            complement(2882..4342)
                     /locus_tag="PK389_00540"
     CDS             complement(2882..4342)
                     /locus_tag="PK389_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002708964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HQY22231.1"
                     /translation="MNFKALMNISRILGYLLMPFSATMLPPVFVALWYHEQTLLHFYA
                     ATVTTLALGFLLWFPFRHYKHEIQSREAFLIVVLLWLGLALTGALPFVFFENPHMHYI
                     DAIFETISGLTTTGATILSELDTLPRSILYYRQQLQFIGGGGIIVLSIAILPMLGVGG
                     MQLFRAEMAGPFKEEKLTPRIAQTAKMLWFIYVGLTILCTLSYWAGGMDVFDAIGYSF
                     STISTGGFGTHDENFSKFQNPLLEYICVVFMLLGAVNFSLHFVAFRRGNLLHYWQDLE
                     FKTYIKLWFWMVLLIGSTLFYFHVFEHTESTLTKTFFQVTSFFTTTGFFSADFNHWPT
                     FVPMLLVFLGLFGGCAGSTSGGIKMIRLLILKKQGEREIQRLIHPNGHYIIKLGYKPL
                     QDQTLDAIVGFFCIFVATFLLLLLILIGMGLDPLTAFATLAASISNAGIGLNCSADNF
                     KSFSDPIKMVLSIAMLAGRLELFTLLVLLTPHYWRY"
     gene            complement(4352..5731)
                     /gene="trkA"
                     /locus_tag="PK389_00545"
     CDS             complement(4352..5731)
                     /gene="trkA"
                     /locus_tag="PK389_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016566811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HQY22232.1"
                     /translation="MKIIILGAGQVGTTLAEHLATEHNDITIIDINGERLRSLQKRMD
                     ILTIAGNAAYPSTLKRGGAEDADMLIAVTNNDETNLVACQVAATLFQIPTKIARIRSL
                     HYLAHEELFGAPAFPIDVLISPEQVISNYIRRLIEYPDALQVLDFANQKLQLVAIKTK
                     TGSGPMVLHPLKELPACLGNLDTEIAAIFRQGAPITPTGSTIIEPNDEVFFLAERQNI
                     RSVMSTLIPLKPANQRIIIAGGGNIGTRLATALEKEFEVKVIEHNPLRIEPLVTHLTT
                     GIVLEGDAADKELLLSENIENTDVFCAVTNNDETNIMSALLAKRLGARKAMALITRPS
                     YLDLVEGGDIDVVISPQQVMVGTLLTHIRRGHIVNVHSLRRGTTEAIEMIVDGNKETS
                     KVVGRYIKDVPLPEEASILAIVRKNRVIMGHHNAMIETGDRVIVLLFDKRKIRKVERL
                     FQENNTRII"
     gene            complement(5728..7032)
                     /gene="rsmB"
                     /locus_tag="PK389_00550"
     CDS             complement(5728..7032)
                     /gene="rsmB"
                     /locus_tag="PK389_00550"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004422858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HQY22233.1"
                     /translation="MNTRALATQAIHAVLSNGHSLSHCLPLFKKKCKTTQDAAFLQVL
                     VFGVIRWYPRLEFIANQLLEKRIKQKDSDVLYLITVGLYQLTDTCVSEHAALAETVEA
                     ASVLNKPWAKGLINAILRSYQRKSEVLKQILQSNETAYYAHPQWLIDALKIDWPDQWK
                     NILMANNTLPPLSLRVNLQKNSRENYLSELEKAGFPAAPIPYTHSGIVLKTPMDIKRL
                     PGFDSGWFSVQDGAAQLSAGLLELAPNLRVLDACAAPGGKTTHMLEMEPNLSELIAID
                     ISPLRTSLISENLARLQLHATIITANASNPTTWWDGKLFDRILCDAPCSAAGVIRRHP
                     DIKYLKKPDDLITLFQQQTQLLNALWPLLKPGGILVYATCSIFTKENDACVQTFLHAT
                     QNAYLLPISMACGLSKTVGHQILPGQNDMDGFYYARIGKKTR"
BASE COUNT         2089 a         1441 c         1531 g         2197 t
ORIGIN      
        1 ttgaaaaaag ctttaatagg cccactaatt cgcgcatcac acttttctgg gatttctggt
       61 tttgttaaag tgaattgtat gccagtttct gtaaagcaca taaaaaaaat tttttcaaag
      121 tctttcagga aaatttccaa acatttacca tacaattttt tgagttgtcc tattgcgttg
      181 ggatctaaag caagtgcgcg attcacaatc gtatctattt gcatagggct aaaatttata
      241 tcctgtatgt aaggcaacga tacccgcgtg aagattttga tattccgttc gttcgaatcc
      301 agcttcacac atcattgatt tgagcgtttc ttgattaggg tgtacacgaa ttgactcggc
      361 aagatactgg taactgtttc tgtcattaca aatccattcc cccagctttg gcagtatcga
      421 gaatgaataa gcattataca atgtgcctag cattttatcg ttaacagttg aaaattccaa
      481 aatcatgact tgtccacccg gttttagcac gcgcgacatt tcagctaagg ctttgttggg
      541 atgtgttaca tttcgtaatc caaaggcaca gataatgcga tcaaaactat tatttgcaaa
      601 ggggatagat tctgcgttgg cttgcgttaa tgcgataggc tgtatgaatc cggcattgat
      661 taagcgttct cgacccttgg ttaacatggc ctcattaata tcagctaaca caatgaggcc
      721 ttctttgccg agcagctttg ccacttgtcg tgtcagatca cctgtgccgc cggctaaatc
      781 tagcacagtg tgatgcggtc gaatgttaga tcgcgctatt gctaagcgtt tccaaaatcg
      841 atgcaatcca aaagacataa gatcattcat cagatcataa cgattggcga ccgaacgaaa
      901 gacagaacct acccgttctg ttttttgatc tgtcgcgatg gattgatagc caaagtgcgt
      961 ggtttcttcg gccatttatt gcgcagcgcg tttcatggac tcgaaaaaga tttcattagt
     1021 tttggtgtct cgcaagcgtt ccaacaagaa ttccatagcg gccaaatcat ccataggatg
     1081 taataatttc cgtaaaatcc acattttttg gagatcttcg ggtttggcaa gaagctcttc
     1141 tctgcgggtg ccagagcgtg cgatattaat tgcgggataa acgcgctttt cagaaatacg
     1201 tctatccaga tgtaattcta gatttcctgt tcctttaaat tcttcgtaga ttacttcgtc
     1261 cattttagaa cccgtatcga tcaaagcggt tgcaataatg gttaagctgc caccttcttc
     1321 aatattacgc gcagcaccaa aaaagcgttt gggtaaatga agggcgttgg catcgacacc
     1381 accggtcaga actttaccag agggggggat aaccgtattg tgtgcacgtg ctaatcgtgt
     1441 aatggaatcg agcagaatca cgacatctcg cttatgttct acaaggcgtt ttgcacgttc
     1501 aataaccata cttgccactt gtacatgacg acttgcgggt tcgtcaaaag tactggcaat
     1561 cacttcgcct cgaacagatc gcgacatttc tgtaacttct tctggacgtt catcaatcaa
     1621 tagtacaatt aaatggcatt ccggatgatt gacggcaata gagtgtgcaa tattttgcag
     1681 cataactgtt ttaccggctt taggcggaga tacaataaga cctcgttgtc cttttccaat
     1741 gggagagacg agatctatca cacggctggt aatatctctg gtgctgccat ctcctgtttg
     1801 cataataagc ggcttgttgg cgtgcaaagg ggttaagttt tcgaaggcta ttttatgacg
     1861 tgtaatttcg ggtggctcaa aattgatggt atcaactttt agcaatgcaa aaaagcgttc
     1921 ttgatcttta ggtgggcgaa ttttaccaaa aatttcgtca ccggttctta aactaaacct
     1981 ccgcacttgg ctgggtgata cataaatatc gtcaggtcct gccagataag agctgtctgc
     2041 ggagcgcaag aatccgtaac cgtctggtaa taattcgagt acaccgttgc cataaatatc
     2101 ttcgcccttt ttagcgtgtg ctttcaggat tgcgaaaata acctcttgct ttcgagctcg
     2161 agcaacatcc tcgatgccga aagtttgggc aatttccatg agttctgtag gtgatttgtg
     2221 tcttaattca gttaaattca taaaaaaatg ttttctttag ttgagttgaa gggttgggtt
     2281 gtatggtgat ccatttgtaa ttaggcgaag cacgttactg ctgcttaagg cttttagatt
     2341 aacactaaaa ttgtaaattg aatagtagat catttttttt ttaatttagc ctaaatgact
     2401 gtctaaaaat tcagataatt gagattttgt taaatgtcct accttagtag cagcgacttt
     2461 gccattttta aataccaata aggttgggat ccctcggata ccaaactttg cagctgtggt
     2521 gttattttca tctacattta attttactat tttaattttt ccagcgtatg tcacggcggc
     2581 ttcctctaga agcggagcaa tcattttaca agggttacac cattctgccc agaaatcgac
     2641 cactacagga gttgtagatt gtaatacatc ggtatcaaaa gagctgtctg tcacgggttg
     2701 tagatgtgtg ctcataagat ggtttttgct cctgcgcttt gtttaaaggg ttgtgtttaa
     2761 cacggattgt ttgtaattaa cgcctttagt gtaactcaaa aatttgattt tatgaacagc
     2821 gatgcaatga aaaatttcag aaagtgtaat acaaccgaca taacttatgg ttccctctcc
     2881 ctcaataccg ccaataatgc ggtgttaaca ataccagtaa ggtaaataat tccaaacggc
     2941 cagcaagcat cgcaatgcta agaaccattt taatgggatc actgaaagat ttaaaattat
     3001 cggcggagca atttaatcca atgccggcat ttgaaatact ggcagcgagc gtggcaaaag
     3061 cagtgagcgg atcaagtccc atgccaatta atatcagtaa taagaggaga aaagtagcaa
     3121 caaaaataca aaaaaatcca acaatagcat ctaaggtttg atcttgtaag ggtttgtatc
     3181 caagcttaat aatataatgt ccgttgggat ggatcagtct ttgtatttcg cgttcacctt
     3241 gtttttttaa gattaagagt cgaatcattt ttatgccgcc agatgttgaa cctgcgcatc
     3301 cgccaaataa acccagaaaa actaaaagca taggcacaaa tgtaggccaa tgattaaagt
     3361 ctgcgctaaa aaaaccggtt gttgtaaaaa aactggtgac ttgaaaaaaa gttttagtta
     3421 acgtgctctc cgtgtgttca aagacatgaa aataaaacaa cgtggatcct attagtaata
     3481 ccatccaaaa ccaaagtttt atatacgttt taaattcaag atcttgccaa tagtgtaaca
     3541 aatttcctcg gcggaacgct acaaaatgca aagaaaaatt aacagcaccc agcaacataa
     3601 aaaccacaca aatgtattct aaaagtgggt tttgaaattt gctaaaattt tcatcatgtg
     3661 taccaaaccc gccggtagag attgtgctaa agctatatcc aatcgcatca aaaacatcca
     3721 tgccgcctgc ccaataactc agtgtgcata gaattgttag gcccacataa ataaaccaca
     3781 acattttagc cgtttgtgca atacgtggag tcagtttttc ctctttaaaa gggccggcca
     3841 tttctgcacg aaacagctgc attcccccaa caccgagcat cggtaaaata gcaatgctga
     3901 gcacgataat cccaccgcca ccaataaatt gcaattgctg acgataatat aaaatggaac
     3961 gcggtagcgt atcgagctcc gaaagaatgg tggctcccgt agttgttagg ccagagatag
     4021 tttcaaatat tgcatcgatg taatgcatat gcgggttttc aaagaagaca aagggcaaag
     4081 cacccgttaa agccaatcct aaccagagca gtacgacaat cagaaaagct tctctggatt
     4141 gaatttcgtg tttataatgt cgaaacggaa accacaataa aaaccccaat gctaatgttg
     4201 ttacggtggc agcataaaag tgtaataggg tctgttcatg gtaccagagc gcaacaaaga
     4261 cgggcggcag cattgttgca ctaaaaggca ttaataagta gcctaaaatt ctagagatat
     4321 tcatcaaagc tttaaagttc ataaaaaacc attaaataat acgcgtgttg ttttcttgga
     4381 atagccgttc gacttttcga attttgcgtt tatcgaataa caatacgatc accctatcgc
     4441 cggtttcaat catggcgtta tggtgtccca taataacgcg atttttgcga acgatggcta
     4501 aaatactggc ttcttcaggt aatgggacat cttttatata acgtccgact actttggagg
     4561 tttctttgtt tccatccaca atcatttcaa tggcttcggt tgtgccgcgt ctgagtgaat
     4621 gtacattgac gatatgaccc cgtcgaatat gcgttagtaa ggtgcccacc atgacttgtt
     4681 gtggggaaat cacgacatca atatctcctc cttctactaa atctaaataa gaaggccgtg
     4741 taatgagcgc catggcttta cgtgcgccca aacgttttgc gagcaatgcg gacataatat
     4801 tggtttcatc gttgttggta acagcgcaaa aaacatctgt attttcgatg ttttcactga
     4861 gtaataattc tttatctgct gcatcacctt ctagtacaat gccagttgtt aaatgtgtga
     4921 ccagtggttc aatgcgtaag gggttatgct ctatcacttt tacttcaaat tctttttcta
     4981 aggctgtggc aagacgtgtg ccaatatttc caccacccgc aataatgata cgttggttgg
     5041 cgggcttaag cggtattagt gtgctcatta cagaacgtat gttttgtcgt tctgctaaaa
     5101 aaaagacttc atcgttgggt tcgatgatgg tgctgcccgt tggtgtaatg ggggcacctt
     5161 gcctaaaaat agcggcgatt tcggtatcaa gattccctaa gcaggcgggt aattctttta
     5221 aaggatgtaa aaccatgggt ccgctgcctg tttttgtttt tattgcgacc aattgtaatt
     5281 tttgatttgc aaaatccaaa acctgtaatg cgtctggata ttcaattaaa cgacgaatat
     5341 aatttgaaat gacttgttct gggctaatga gtacatcaat cggaaaagca ggtgctccaa
     5401 aaagttcttc gtgtgcaaga taatgcaaag atcgaatgcg ggcaattttt gttggaattt
     5461 gaaaaagtgt cgcagccact tggcatgcaa ctaaattggt ttcatcattg ttggtaaccg
     5521 caataagcat atctgcgtct tcagcacccc cgcgttttag tgtgctcgga taagcagcat
     5581 ttcctgcaat cgttaaaata tccatgcgtt tttgtaacga tcgtaggcgt tcaccattga
     5641 tgtcgataat ggtaatatca ttatgttcgg tagcaaggtg ctctgccaag gtagtgccta
     5701 cttgtcctgc acctaagata ataatcttca tctggttttt ttcccaatgc gtgcgtagta
     5761 aaagccatcc atatcgtttt gtccaggtaa tatttggtgg ccaacggttt ttgacagccc
     5821 acatgccatc gaaattggaa gtaaataagc attttgtgtc gcgtgcaaaa atgtttgtac
     5881 acatgcatca ttttcttttg tgaagataga acatgttgca tacaccagta tgccgccggg
     5941 ttttaaaagc ggccatagcg cgtttaggag ttgtgtttgt tgttgaaaaa gcgtaattaa
     6001 atcgtcaggt ttttttaaat atttaatgtc tggatgtctt cggataacac cggcagcaga
     6061 acagggagca tcacataaaa tgcgatcaaa caatttaccg tcccaccatg tggttggatt
     6121 gctcgcattt gcggttataa tcgttgcgtg taattgtaga cgcgctaagt tttcagaaat
     6181 caatgatgtg cgcagaggtg atatatcaat tgcaattaat tcagaaagat ttggctccat
     6241 ttcaagcatg tgggttgttt tgccacctgg cgctgcacaa gcatctaata cgcgaagatt
     6301 aggcgcaagc tctaacaatc ctgcagacag ctgcgctgcg ccatcttgaa ccgagaacca
     6361 gccggaatca aagcctggta accttttaat atccatgggt gtttttaata caataccgga
     6421 atgcgtatag ggaatgggtg cagcaggaaa gcctgctttc tcgagttcgc tgaggtagtt
     6481 ttcacgagaa tttttttgca gattaacacg caaactaagc ggcggaagtg tattgttagc
     6541 cattaaaata tttttccact gatctggcca atcaatctta agtgcatcga ttagccactg
     6601 tggatgtgca taataagcgg tttcgttgga ctgtaatatt tgttttagaa cttctgattt
     6661 gcgttgatat gatcggagta tcgcgtttat taaacctttt gcccatggtt tgtttaacac
     6721 gcttgccgct tcgacagttt cggccaatgc ggcatgttcg gatacgcatg tgtcagttaa
     6781 ttgataaagt ccaactgtaa tcaaatacag tacgtcgctg tctttttgtt tgatacgctt
     6841 ttctaataat tgatttgcaa taaattcgag acgtgggtac cagcgaataa cgccaaaaac
     6901 caagacttgc aaaaaagcag catcttgggt tgttttgcat ttttttttaa acaatggcaa
     6961 acagtgactt aaagaatgcc cattgcttaa aaccgcgtga attgcttggg tggccagggc
     7021 acgcgtattc atatagaatg ttttttcgca agacggttta tatgggacat ctatgttata
     7081 taccttaact acatcttcgt gggcagagcc attttccact gtagagcagg aaaatgcaat
     7141 aaccgcgctt gagaatggaa aaatacctta ttttccaaac ctccttcgag tttagcgctg
     7201 ctgaacaggc attgctaacg cccaaggtgc taggcgctgg ctttaaaaat atcagttt
//
LOCUS       DAORVT010000013         7152 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_6364_length_7152_cov_3.836832, whole genome shotgun sequence.
ACCESSION   DAORVT010000013 DAORVT010000000
VERSION     DAORVT010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7152
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6364_length_7152_cov_3.836832"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1497)
                     /gene="parC"
                     /locus_tag="PK389_00555"
     CDS             complement(<1..1497)
                     /gene="parC"
                     /locus_tag="PK389_00555"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008739326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HQY22234.1"
                     /translation="MSKAKMVKVSNDEQQSLKAFTEHAYLDYSMYVILDRALPNIADG
                     LKPVQRRIIYAMSELGLIATAKYKKSARTIGDVLGKFHPHGETACYEAMVLMAQSFSY
                     RYPFVDGQGNWGSPDDPKSFAAMRYTEARLSPYANALLSELEHGTVDWASNFDGTLEE
                     PKLLPARLPNVLLNGTTGIAVGMATDIPSHNLGEIVSALIYCLENPDASIEQLCNIVQ
                     GPDFPTEAEIITPKSDILKMYQSGGGSLRMRAVYTIDEGDIIITALPHLVSGSKVLEQ
                     IAALMQAKKLPLVSDLRDESDHEEPTRLVITPRSNRVDVNSLMAHLFATTDLERTYRV
                     NLNMIGLDGRPRVKNLKEILFEWLIFRQETVRRRLSYRLEKVITRLHVLDGFLIAFLN
                     LDEVIRIIRYEDEPKRMLMAKFGLSDTQTEAILDLKLRHLAKLEENKIRAEQKELMEE
                     RINLEKTLDSDQRLKTVLKKELEQDLKTYGDKRRSPIVVRFDAKVLQEI"
     gene            complement(1490..3382)
                     /gene="parE"
                     /locus_tag="PK389_00560"
     CDS             complement(1490..3382)
                     /gene="parE"
                     /locus_tag="PK389_00560"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HQY22235.1"
                     /translation="MAKKSYTAESIEVLSGLDPVRKRPGMYTDTARPNHLAQEVIDNS
                     VDEAISGYANSIKIVLHADGALEVSDNGRGMPVDIHPQHKVSGIELILTRLHAGAKFS
                     GKDYRFSGGLHGVGVSVVNALSKRLEVWVKRQGQVYHMAFEQGEAVSKLKVIGKCDKN
                     EQGTTVKFWPEPKYFDSSKILSKPLKHLCQAKAVLCPGLTVRLEDHQNNEGPFEWCYA
                     GGLKAYLLEQVGNRAILPEEPFEGLFEGNDEIASFAVVWTPFDPESFQESYVNLVPTA
                     QGETHVNGLRTGLLEAMREFCELRNLLSRGVKLAPEDIWDKINFVLSVKLLDPQFSGQ
                     TKERLSSRQVAAFVSGVVKDSFSLWLNQHVQIATQLAEFVIETAEKRLRQGKMIARKK
                     ITQGPALPGKLADCTLQDLNRCELFLVEGDSAGGSAKQARDRTFQAVMPLRGKILNTW
                     EVASLEVLASQEVHDISVAIGVDPGVETLEKLRYGKICILADADSDGQHIATLLCALF
                     VKHFRSLVEAGHLYVAMPPLYRIDAGKEVYYALDDSEKQGIIDRIVAEKAKAKIVVQR
                     FKGLGEMNPIQLRETTMAPDTRRLVQLKLIKEEDTVNFMDMLLAKKRAADRKQWLESK
                     GNLADV"
     gene            complement(3391..4017)
                     /locus_tag="PK389_00565"
     CDS             complement(3391..4017)
                     /locus_tag="PK389_00565"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQY22236.1"
                     /translation="MTFKSRILGYLAYYITLLLRLTLRVVISKHPEYNSKTQYLFAFW
                     HGKQLLPVFQLQNHHTRRAVLVSPSRDGDMLTVWLKNLGYEVIRGSSRKQNIRALAGM
                     IRKLKAGYSLGFGIDGPIGPIYKVKPGMTHMAQKLRIPIIPVGSAFSRSWVFEKAWDK
                     YEIPKPFAKAVFYLGKPFIIEKEVDLAISNGMLEGLIIQAEITARALL"
     gene            complement(4014..4481)
                     /locus_tag="PK389_00570"
     CDS             complement(4014..4481)
                     /locus_tag="PK389_00570"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008044785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HQY22237.1"
                     /translation="MRIALIAAMSENGVIGNHNALPWHLPEELEYFREKTRDKPVIMG
                     RKTFESIGSKPLPNRLNIILTHALHFTALDCKVVHSVEDALKAAGDCEEIMVIGGAEI
                     YEAFLSVASRIYLTVIHQTCIGDTVFPTVDWFAWKTLSEEKRDGFTLKVFDKR"
     gene            complement(4490..5284)
                     /locus_tag="PK389_00575"
     CDS             complement(4490..5284)
                     /locus_tag="PK389_00575"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HQY22238.1"
                     /translation="MQQYLALMQHVLQHGSKKMDRTGTGTLSVFGYQMRFDLNQGFPL
                     VTTKKVHLRSIIHELLWFLRGDTNIKSLNAAGVTIWDEWADENGDLGPVYGKQWRSWP
                     DSKGGIIDQMSGLIDMLLQNPDSRRLIVSGWNVAEISNMALPPCHCLFQFYVAEGRLS
                     CQLYQRSADIFLGVPFNIASYALLTHMIAQVVGLKLGDFVHTLGDAHLYLNHLEQARE
                     QLNRTPYPLPTMQINPTVNSIFDFQYEDFNVINYQCHPRISAPIAV"
     gene            complement(5306..6097)
                     /gene="lgt"
                     /locus_tag="PK389_00580"
     CDS             complement(5306..6097)
                     /gene="lgt"
                     /locus_tag="PK389_00580"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_820532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HQY22239.1"
                     /translation="MLTFPDINPVALQIGPLAIHWYGVMYVIGFVGVFWSCYVRRTES
                     HSGSAPWERDEIVDLLFYGAMGVIFGGTLGYLLFYQPQVFFDDPLRLFRFWEPGRSFH
                     GGLLGVLLAVFIFGKVHRRHFWAITDFIAPAIPIGIMAGRLGNFINGELWGRVTNMPW
                     GIVFPYAGAMPRHPSQLYEFGLEGLSLFLILNVYAKKTRRTGAVSGLFLVCYGLFRFG
                     IEFFREPDFDQGFVALGWMTKGQILSLPMVMIGLFLLFYSKKYHK"
     gene            complement(6123..6641)
                     /locus_tag="PK389_00585"
     CDS             complement(6123..6641)
                     /locus_tag="PK389_00585"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HQY22240.1"
                     /translation="MIDSSGFRSNIGIILANHAGRVFWAKRVRRDAWQFPQGGMNPDE
                     TPEQTLFRELEEEVGLKEQDVTILGRTDRWLRYRLPTRLIRKTVPICVGQKQLWFLLR
                     LEGDDSLIRLDKTHKPEFDDWQWVSYWFPLRQVVSFKREVYRRALKELAPLLFWRHAE
                     QALGDRPLDYLL"
     gene            6871..>7152
                     /locus_tag="PK389_00590"
     CDS             6871..>7152
                     /locus_tag="PK389_00590"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HQY22241.1"
                     /translation="MGAELSTKLLEAVNNRSNSMVKQLLQDKTIRQIANANDNEALRL
                     AAKNNDKEIVKMLLQLPVVSKNAHAKDNEALVWMIFHSKDDGNGPDYFDE"
BASE COUNT         2169 a         1698 c         1277 g         2008 t
ORIGIN      
        1 aatctcttgt agtactttgg catcgaagcg gacaacaatc ggagaacgtc gcttatcgcc
       61 ataggttttg agatcttgct ctaattcttt tttgagcacc gtttttaagc gctgatctga
      121 atccaaggtt ttttccaaat taatgcgttc ttccatcaat tctttttgct cggcacgaat
      181 tttattttct tctaacttcg caagatgtct taattttaaa tccaatattg cctcggtttg
      241 tgtatcactc aatccaaact ttgccattaa catacgttta ggttcgtctt cataacgaat
      301 aatacggata acttcgtcaa gatttaaaaa tgcaattaaa aatccgtcta acacatgtaa
      361 gcgcgtgata actttttcga gtcgatacga caatcgacgg cgaaccgttt cttgtctaaa
      421 aattaaccat tcaaataata tttcttttaa atttttaacg cgtggtctac catctaatcc
      481 aatcatattt aaattaacgc gataggtgcg ttctaaatcc gttgtcgcaa acaaatgcgc
      541 catcagtgaa ttaacatcga ctcgattaga gcgcggcgta ataaccaagc gtgtgggttc
      601 ttcatgatca gattcatcgc gcaaatcaga cactaaaggt aactttttgg cttgcatcag
      661 cgccgcgatt tgctctaaaa ctttactacc agaaaccaga tgcggcaacg ctgtaataat
      721 aatatcaccc tcgtcgatgg tataaactgc gcgcattctt aatgatcccc cccccgattg
      781 atacattttt aaaatgtcgc tttttggggt aataatttcg gcttccgttg ggaaatcggg
      841 tccctgaaca atgttacaaa gttgttcaat agacgcatct ggattttcaa gacaataaat
      901 taacgctgaa acgatctcgc ccaaattatg ggatggaata tctgttgcca taccaacggc
      961 aatgccggtt gtgccgttaa gcaatacatt tggcaaacgc gcaggcaata atttaggttc
     1021 ttccaaagta ccatcaaaat tcgaagccca atccaccgta ccatgttcta attcgcttaa
     1081 taacgcattc gcataaggag acaatcgtgc ttctgtgtaa cgcattgcag caaacgattt
     1141 tggatcatcg ggtgaccccc aatttccttg accatccaca aagggatatc gataagaaaa
     1201 tgattgcgcc attaatacca ttgcttcata acaagcggtt tctccgtgcg gatgaaattt
     1261 ccctaaaaca tccccaatgg tacgcgctga ttttttatat ttggctgtcg caattaaacc
     1321 gagctccgac atcgcataaa taatacgtct ctgtaccggt tttaaaccat cggcaatatt
     1381 gggcaaagcg cgatctaaaa taacatacat cgagtaatcc aagtaggcgt gctcggtgaa
     1441 tgctttaaga gattgttgct cgtcgttaga gactttaacc atttttgctt tagacatctg
     1501 ctaagttacc tttactttct aaccattgtt ttctatcggc tgcacgtttt ttagccaata
     1561 gcatatccat aaaattgacg gtgtcttctt cctttatcaa ttttaattgt actaagcgtc
     1621 gtgtatcagg cgccatggtg gtttctcgaa gttgtatcgg attcatttca cccaagcctt
     1681 taaagcgttg caccacgatt tttgcttttg ctttttcggc cacaattcta tcaataatcc
     1741 cctgtttttc tgaatcatct agggcataat acacttcttt gcctgcatcg atacgataca
     1801 aaggcggcat tgccacatac aagtgacctg cttccactaa ggatctaaaa tgctttacaa
     1861 ataaagcgca cagcaaggtc gcaatatgtt gtccatctga atccgcatct gccaaaatac
     1921 aaattttacc ataacgcaat ttttctaatg tctcaacgcc gggatcaaca cctatcgcaa
     1981 cagaaatatc atgaacttcc tgagaagcta acacttcaag tgatgcgact tcccaagtat
     2041 ttaatatctt tcctctgagc ggcataacgg cttggaaggt gcgatcgcgt gcttgcttag
     2101 cagatccacc tgctgaatcc ccttccacta aaaacaattc gcatcgattg agatcttgca
     2161 gcgtacaatc ggctaatttt ccaggtaagg caggaccttg cgtaattttt ttacgtgcaa
     2221 tcattttgcc ttgacgcaac cgtttttccg cagtttcaat aacaaattct gcaagctgcg
     2281 ttgcaatttg aacatgttga tttaaccata aactaaatga atcttttaca acacctgaaa
     2341 caaaagcagc tacttgtcga gaagataatc gctctttggt ttgccctgaa aattgcggat
     2401 ccaacaattt aaccgacagc acgaaattaa tcttatccca aatatcttct ggcgctaatt
     2461 taacacctct ggataataaa tttcgaagct cacaaaattc gcgcatggct tctaacaaac
     2521 ccgtgcgtaa cccattaacg tgggtttccc cttgcgcagt tggcaccaaa ttgacatagc
     2581 tttcttgaaa tgattcagga tcgaatggtg tccacacaac cgcaaagctg gctatttcat
     2641 cattcccttc aaataatcct tcaaaaggct cttcgggtaa aatggctcga ttacccactt
     2701 gctctaaaag ataggcttta agaccaccgg cataacacca ttcaaaaggt ccttcgttat
     2761 tttgatgatc ttcaagacga acagtcaacc ctgggcataa tacggcttta gcctgacata
     2821 agtgtttaag cggtttgctt aaaattttgg aagaatcgaa atatttaggc tctggccaaa
     2881 atttaaccgt ggtgccttgc tcatttttat cacattttcc aatcactttc agcttagata
     2941 cggcttctcc ttgctcaaag gccatgtggt aaacctggcc ttgacgtttt acccaaacct
     3001 ctaatcgctt agataaggca ttcacaactg aaacgcctac accatgcaat ccacccgaaa
     3061 aacgataatc ttttccagaa aatttcgcac ctgcatgcaa acgcgttaaa atcaattcta
     3121 taccagagac tttgtgttgt ggatgaatat ccactggcat cccgcgacca ttatcagaga
     3181 cttctaatgc accatcggca tgcagcacaa tttttatgct attcgcatac ccactaatcg
     3241 cctcatccac actattgtcg ataacttctt gcgcaagatg attggggcgt gcggtatcgg
     3301 tatacatgcc cggacgctta cgtaccggat caagcccact taacacctcg atagattcag
     3361 cggtgtaact cttttttgcc ataaaaaatc ctacaaaagt gctcttgctg ttatttctgc
     3421 ctgtattatc aatccttcca gcataccatt actgatggca agatcgactt ctttttcaat
     3481 aataaacggt ttacccaaat aaaaaacagc ttttgcaaaa ggttttggga tctcgtattt
     3541 atcccaggct ttttcaaaaa cccagctacg gctaaatgca ctgccaacag gaataatggg
     3601 aatccgcaat ttctgagcca tgtgtgtcat ccccggtttg actttataaa taggaccaat
     3661 gggtccatcg attccaaagc ccaatgaata tcccgctttc agttttcgga tcatgcctgc
     3721 caacgcacgg atattctgtt tccgagaaga accgcgaatg acctcgtacc ctaaattttt
     3781 taaccaaacc gttaacatgt cgccatctcg agagggactg actagcaccg cccgacgcgt
     3841 atgatgattt tgcaattgaa aaacaggtaa caactgtttt ccatgccaaa atgcaaacaa
     3901 atattgcgtt ttcgaattgt attccggatg tttggatatt accacccgca atgtcaacct
     3961 caataacagc gtaatataat atgcaagata tcctaaaatt cggcttttga atgtcatctt
     4021 ttatcaaata cttttaacgt aaatccatct cgcttttcct ctgatagagt tttccaggca
     4081 aaccaatcaa ctgtcggaaa aaccgtatca ccaatacatg tttgatgtat caccgttaaa
     4141 taaattctag aagcaacgga taaaaaagct tcgtaaattt cagcgccacc gatcaccatg
     4201 atttcttcac aatcacctgc cgcctttaat gcatcctcta ctgaatgcac aactttgcaa
     4261 tctaaagctg taaaatgcaa tgcatgggtt aaaataatat ttaatcgatt tggcaagggt
     4321 ttactaccta ttgattcaaa cgtcttacgt cccataataa ccggcttatc acgagttttt
     4381 tcgcgaaaat attctaattc ttccggcaag tgccacggga gcgcattatg atttccaatc
     4441 acgccatttt cactcatcgc tgcaattaat gcaatacgca tatttttctc tacaccgcta
     4501 taggcgcaga aatgcgtgga tgacattgat aattaatcac attaaaatcc tcatattgaa
     4561 aatcaaaaat tgaattgaca gttggattaa tttgcatagt aggtaatgga tatggcgttc
     4621 tgttcagttg ctctcttgct tgttctaaat gattcaaata gagatgtgca tcgcctaatg
     4681 tatgcacaaa atcaccgagc tttaagccta ccacttgcgc tatcatatgc gttaataatg
     4741 catatgaagc aatattaaag ggcaccccta aaaatatatc ggcactgcgc tgatacagct
     4801 gacaagacaa acgtccttct gcaacataaa actgaaaaag acaatggcag ggcggtaacg
     4861 ccatatttga aatttccgcc acattccaac cactcacaat taatcgacgc gaatccggat
     4921 tttgtaagag catgtcgatt aagccggaca tttgatcaat tatgccaccc ttgctatctg
     4981 gccaagatcg ccactgcttt ccgtagacag ggcctaaatc tccattttca tctgcccatt
     5041 catcccaaat cgtcacgccg gctgcattta acgatttaat attcgtatca ccccgtaaga
     5101 accataataa ctcatgaata atagagcgca aatgcacttt cttagtcgtc accaatggga
     5161 atccttggtt taaatcaaat cgcatttgat atccaaaaac gcttaacgtc ccagtacccg
     5221 tacgatccat ttttttagag ccgtgttgaa gcacatgctg catcaaagct aaatattgct
     5281 gcataccccc tcctgatgat tgatatcatt tatggtattt tttgctataa aataacagaa
     5341 acaatcctat catcaccatc ggcaaagaca agatttgtcc ttttgtcatc caccccaatg
     5401 caacaaaacc ctgatcaaaa tcgggctcgc ggaaaaattc tatcccaaaa cgaaataatc
     5461 cgtaacacac taaaaacaaa ccagatacag cgccagtcct tctggttttt ttagcgtaca
     5521 cattcaaaat aagaaagaga ctcaatccct ctaatccaaa ttcatacaac tgtgaaggat
     5581 gtcgcggcat tgcgcccgca tatggaaaga caatgcccca aggcatgttt gtgacgcgtc
     5641 cccacaactc accgttaata aaattaccca gacgtccggc cattatccca atcggaatcg
     5701 ctggcgcaat aaaatccgtt atcgcccaaa aatgtcgtcg atgaactttg ccaaatataa
     5761 agacagccag aagaacaccc aacaagccgc catgaaaaga acgcccgggt tcccaaaaac
     5821 gaaaaagtct gagcggatca tcaaaaaaaa cctgtggttg gtaaaatagt aaataaccta
     5881 aggtgccgcc aaaaatgacc cccatggcgc cataaaataa taaatcaaca atctcgtctc
     5941 tttcccaagg cgcggatccc gagtgagatt cggtacgccg cacataacaa gaccaaaaca
     6001 caccgacaaa acctatcaca tacataaccc cataccaatg gatggccaat gggcctatct
     6061 gtaatgcaac gggattgata tcagggaaag tgagcatgag agaagtatat cgcaaattgg
     6121 cgctacaaca aataatctaa aggtcgatcc ccaagtgcct gctccgcgtg cctccaaaaa
     6181 agcaaaggcg caagttcttt gagtgcacgt ctgtaaactt cacgtttaaa tgagaccact
     6241 tgccttagcg gaaaccaata actcacccat tgccagtcat caaactctgg cttgtgtgtt
     6301 ttatcaagtc ggatgagact atcatcacct tctaaccgta ataaaaacca taactgtttt
     6361 tgcccaacgc aaatgggcac tgtctttcgt atcaaacgag taggcagtcg atatcttagc
     6421 caacgatccg tacgacctaa aatagtcaca tcttgctctt ttagaccgac ttcttcttcc
     6481 aattctctaa aaagcgtctg ctctggggtt tcatctgggt tcatccctcc ctgtggaaac
     6541 tgccaagcat ctcgacgaac acgctttgcc caaaagacgc ggccagcatg attagcgaga
     6601 ataataccga tattagatcg aaaaccactg gaatctatca cgcacgcctt cccgttttat
     6661 cgtaacaagc agaaatcccc ccgatcattg atgacaaccc aagccttgca aagccttaac
     6721 gatctcggtt gcgctttcaa tatttggtat caggaaacac aagcggttca caggaacact
     6781 cctccaccgc atttgataat gatagcagag ttacggatgt actatacttt taatgaaaac
     6841 tgactaacta tataggctgg agatgataaa atgggcgcag agttaagcac aaaactactg
     6901 gaggctgtaa ataaccgtag taattctatg gtcaagcaat tattgcagga caagacaata
     6961 cgtcagattg ccaatgctaa cgataatgaa gcgttgcgat tggcggccaa gaataacgac
     7021 aaggaaatag tcaagatgct gttacagctc ccagttgtga gtaaaaatgc tcatgccaag
     7081 gataatgaag cgttggtatg gatgattttc catagcaagg atgatggtaa cgggccggat
     7141 tatttcgatg aa
//
LOCUS       DAORVT010000014         7005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_6587_length_7005_cov_3.783741, whole genome shotgun sequence.
ACCESSION   DAORVT010000014 DAORVT010000000
VERSION     DAORVT010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7005
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6587_length_7005_cov_3.783741"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..1010)
                     /gene="hemF"
                     /locus_tag="PK389_00595"
     CDS             complement(57..1010)
                     /gene="hemF"
                     /locus_tag="PK389_00595"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020393528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HQY22242.1"
                     /translation="MDNVERDSVDACAVRQYLLALQRDLCQQFSSEDGKGQFESDPWT
                     RPEGGDGISCVLTDGVVIERGGVNFSHIFGDALPAAALPHHPELDGYQFEAMGVSVVI
                     HPHNPYVPTAHFNVRFFLAKKSGAPSVWWFGGGFDLTPYYPFTEDCTHWHRVAAAACQ
                     PFGEDLYARFKKWADNYFYLKHRKESRGIGGIFFDGLNEWSFETCFAFIRSVGDHFIK
                     AYRPIITKRKTFVFGQAERAFQLYRRGRYVEFNLIYDRGTLFGLQFGGRIESILMSLP
                     PIVRWQYNGYSDLENPENSENPQAKLMKFLEPQDWLGMTVA"
     gene            complement(1015..3330)
                     /gene="topA"
                     /locus_tag="PK389_00600"
     CDS             complement(1015..3330)
                     /gene="topA"
                     /locus_tag="PK389_00600"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HQY22243.1"
                     /translation="MAKNLVIVESPAKAKTLKKYLGKDFEILASYGHVRDLVPKTGAV
                     DPAHDFAMKYQIIEKNAKHLETICAELKNATSLYLATDPDREGEAISWHLYELVKERK
                     ILKDKPVHRVVFHEITKTAVQAAIQKPRDLSMDLVNAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRMIVEREEEIEKFITQEYWTVEADLKSKKHEFSARLVEYAGK
                     KFEQFSVDNEKDAREIENALRLLAKGKLKVEKITKSQRKRNPTAPFTTSTLQQEASRK
                     LGFTTQRTMRVAQQLYEGIDVGEGAVGLITYMRTDSVALAKEAVGEIRDLILARYGES
                     ALPKEPRVYKTKAKNAQEAHEAIRPTMAQLIPDKIKSALTLDQYKLYNLIWKRAIACQ
                     MIHATIDAVAVDLSCGEGNRFRANGSIVSSPGFMQVYLEGVDDAKTTETDGERLLPPL
                     KEGEMVDLIALHADQHFTDPPPRFTEASLVKALEEHGIGRPSTYASIISTLQHRVYVE
                     LESRQFKPTDVGCIVSKFLTQYFTQYVDYDFTAKLEDELDAVSRGEKEWVPLLANFWK
                     PFIDLIHTTEETVKRQDVTHEALDEKCPKCTKPLSIRLGKRGRFIGCTGYPECDYTRN
                     LEEDRETSLLPPEVVQGRLCPSCDSELIIKQGKYSKFIGCSQYPKCKFSESLDKPVDT
                     EVTCPVCQKGSLVKRKSRYGTFFYSCNQYPDCKYAVNSPPVAGPCPTCQWPILMIKTT
                     KRRGTEKVCPQKECTFAEPYGDPAADPVVES"
     gene            complement(3413..4498)
                     /gene="dprA"
                     /locus_tag="PK389_00605"
     CDS             complement(3413..4498)
                     /gene="dprA"
                     /locus_tag="PK389_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020482933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HQY22244.1"
                     /translation="MSFKTEQRRFLLALLRISGMGPVKLSEILKKYTDLAEAFDAAGN
                     CKVIKGIADWSRVDQDLAWMSGKDCHIITQYDALYPPLLKETHGAPPVLFVQGNLKLL
                     ARPQIAIVGSRNPTPIGCENAMKFAHHFSCIGFTVTSGLAIGVDGAAHKGALSGVGNT
                     IAVLGNGLDSIYPAVHRALAREIVEKGGALVSEFPIGTPPAHSHFPRRNRVISGLSVG
                     VLVIEATLNSGSLITAKVALDQGREVFAIPGSIHNPLAKGCHALIRQGAKLVETAEDV
                     SEELGEILKYVIRSKEELNKKPALEKNLSVTEEALLSQVGYDSTSVDVVVMRSGLTAK
                     AVSIMLLELELKGLVTSVPGGYARLLA"
     gene            complement(4518..5363)
                     /locus_tag="PK389_00610"
     CDS             complement(4518..5363)
                     /locus_tag="PK389_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22245.1"
                     /translation="MMLRAGCGVVTLLLISCIAYAGTGTTNDKLNTVKLKPKVRESKI
                     IKPVTTLSLETLRPFIKMSVILEDKKQFLKAPSVIAEDKNRIEGGPGTVLYVLGTKCR
                     EDSAYGIYRAGRSYKHPCTGEKLGFEAISIGSAELIKPGDPAVFDVVTATESIEVGSR
                     LLPSVASSLPSTLTIHPAKPMAAEGFILSVRDGINQAGRNQVVLISLGQREGLVAGDT
                     LDIMQNGRKIVDPTSKGWRKCFVQLPDLRIGSLLVFQTYEKLSLGLILESTEMISLLD
                     KVKSP"
     gene            5472..5978
                     /gene="def"
                     /locus_tag="PK389_00615"
     CDS             5472..5978
                     /gene="def"
                     /locus_tag="PK389_00615"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HQY22246.1"
                     /translation="MSLLNILQFPDPRLKKIATPVAVFDDELLSLITNMFETMYEAQG
                     VGLAATQVNVHKRVIVLDVSNEQNQPLALINPEMITKVGILEREEGCLSFPGVYAKVK
                     RYQDIEIKYRDRNGKLQNLKANGGLLSACIQHEIDHLDGITFFDHLSLLKRELLEKKL
                     DKNRKRAL"
     gene            5975..6904
                     /gene="fmt"
                     /locus_tag="PK389_00620"
     CDS             5975..6904
                     /gene="fmt"
                     /locus_tag="PK389_00620"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020582233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HQY22247.1"
                     /translation="MRIVFAGTPEFAVQPLKALLDSKHSVIAVFTQPDRPAGRGQTLH
                     ASPVKALALSHHIPIYQPILLKNPEEQAKLHALQPDLLIVVAYGLLLPKAVLDIPKFG
                     CINIHASLLPRWRGAAPIQHAILAGDKTTGICTMQMDEGLDTGDVLLKISCTIAPQET
                     SKTLHDKLATLGAAALLETLEKFEHNALVPEKQDPTTATYATKISKTDAKIDWQESAD
                     VIDRKIRAFNPWPVAFAELSGQTIRIWSAKLNLTATVNNKIKPGTIIEIHKDSMDVAT
                     GDGVLTLLEIQLPGGKRLPVAEILHAKKALFQG"
BASE COUNT         2016 a         1667 c         1315 g         2007 t
ORIGIN      
        1 tgaagaataa ttaatctaga atgtttattc tagtataaaa ttgcagttag acaactttag
       61 gcaacagtca tcccaagcca atcctgtggc tctaagaatt tcattaattt tgcttgtggg
      121 ttttctgaat tttctgggtt ctctaggtca gagtagccat tgtattgcca acgaacgatt
      181 gggggtaaag acatcaggat agattcgatg cggccgccaa attgtaatcc aaataaagta
      241 cctctatcgt aaatcagatt aaattcaaca taacgtccgc ggcgatacaa ctgaaaagcg
      301 cgttctgctt gaccaaacac aaatgttttt cgtttcgtta taataggtcg ataggctttt
      361 ataaaatgat cgccaacaga tcggataaat gcaaaacacg tctcgaaaga ccattcgttt
      421 aaaccatcaa aaaaaattcc accaatcccg cgtgactctt ttcgatgttt taaataaaaa
      481 taattgtctg cccatttttt aaagcgtgcg tacagatcct caccaaaggg ttgacaagca
      541 gcagcggcta ctcgatgcca atgcgtacaa tcttctgtaa atgggtaata cggtgttaaa
      601 tcgaacccac ctccaaacca ccaaacagac ggagctcccg acttttttgc tagaaaaaaa
      661 cgcacattga aatgcgctgt tggcacatac ggattgtgtg gatggattac gaccgaaaca
      721 cccattgctt caaactgata tccgtctaat tcggggtgat gaggtaatgc tgcagcgggt
      781 agagcatctc caaatatatg cgaaaaatta acgcccccac gctcgataac aacgccatcc
      841 gtcaatacac acgaaatgcc atcaccgcct tctggtcgtg tccaaggatc cgattcaaac
      901 tgaccttttc cgtcttctga tgagaattgt tgacagagat ctctttgtaa tgccaagaga
      961 tattgtcgaa cagcacaagc atcaacacta tcacgctcta cattatccat atttctaaga
     1021 ctctacgacg ggatctgcgg caggatcacc atacggctct gcaaacgtgc actctttttg
     1081 agggcaaacc ttttcggtac cgcgacgttt agtggtttta atcattaaga tgggccactg
     1141 gcatgtcgga catggtcctg caaccggggg gctgttaact gcatatttac aatcgggata
     1201 ttgattacag gaataaaaga atgtgccata tcgcgacttt cttttcacta atgagccctt
     1261 ttgacataca ggacatgtga cttctgtatc gaccggctta tctaaagatt cactaaattt
     1321 acattttgga tattggctgc aaccaataaa tttactatat ttcccttgtt ttataatcaa
     1381 ttcagaatca caacttgggc ataaacgacc ctgtacaact tcgggtggca acaaagaagt
     1441 ttctctatct tcttctagat tgcgcgtgta atcacattct ggataccccg tacatccaat
     1501 aaagcgcccg cgtttaccta aacgaataga aagcggtttg gtacattttg gacacttttc
     1561 gtctaatgct tcgtgagtaa catcttgtcg tttaacggtt tcttctgttg tgtgaatcaa
     1621 gtcaataaac ggcttccaga aatttgccaa caaaggaacc cactcttttt cgccgcgaga
     1681 aacagcatct aattcatctt ccaacttggc tgtaaaatca taatccacat attgtgtaaa
     1741 atattgcgtt aaaaatttgc tgacaataca cccaacatcg gttggcttaa attgacgact
     1801 ttctagctcc acataaacac gatgctgaag cgttgaaata atagaggcat aggttgaagg
     1861 tcgcccaatg ccatgttcct ctaatgcttt aaccaaagac gcttccgtaa agcgaggagg
     1921 cggatctgta aaatgttgat ccgcatgcag cgctatcaaa tcaaccattt cgccttcttt
     1981 taagggtgga agcagtcgtt cgccatctgt ttctgtggtt ttggcatcat caacaccttc
     2041 taaataaacc tgcataaaac ccggtgagga gacaatagag ccatttgcgc gaaaacgatt
     2101 gccttcccca caagaaagat caacagcaac ggcatcgata gtagcgtgta tcatctgaca
     2161 agcaatcgct cgcttccaaa ttaaattata cagtttgtat tgatcgagcg ttaaagcgct
     2221 tttaatttta tcaggaatca gttgcgccat cgtaggacga atcgcttcgt gtgcttcttg
     2281 cgcattttta gctttggttt tatacacacg cggttctttg ggtaacgcgc tttcaccata
     2341 acgcgccaaa attaagtctc gaatttcacc aacggcttct tttgccaacg caacagaatc
     2401 ggtacgcata taggtaatta aaccaaccgc gccttcacct acatcgatac cttcatacaa
     2461 ttgctgcgcc acacgcatcg ttctttgcgt tgtaaatcca agcttgcgag aagcctcttg
     2521 ttgcagtgtc gaagttgtaa atggcgccgt tggattacgt tttctttggc ttttggtaat
     2581 tttttcgact ttaagcttac ctttagccaa caaccgaagc gcattttcaa tttctctggc
     2641 atctttttcg ttatcaacag aaaactgctc aaacttttta cccgcgtact caaccaatcg
     2701 tgcgctaaat tcgtgctttt tcgatttaag atccgcttct actgtccaat attcttgtgt
     2761 tataaatttt tctatttctt cttctctttc cacaatcata cgaagcgccg ggctttgcac
     2821 acgtcctgca gataatcctc gacgtatttt tttccacagt aaaggcgaca aattaaaacc
     2881 aaccagataa tctaatgctc tacgtgcttg ctgcgcattc accaaatcca tcgataagtc
     2941 tcttggtttt tgaatagcag cttgaaccgc agtttttgtg atctcatgaa acacgacccg
     3001 gtgaacgggt ttatctttta aaatctttct ctctttaaca agctcgtata aatgccatga
     3061 aatagcctct ccttctcgat ccggatcagt cgcgagatat aaagacgtgg cattttttaa
     3121 ctctgcgcaa attgtctcta aatgcttagc atttttttca atgatttgat acttcattgc
     3181 aaaatcatgc gcgggatcga ccgctccggt tttaggaacg agatctcgca catgcccata
     3241 ggaggcaaga atttcaaaat ccttccccag atatttcttt aaggttttag ccttagctgg
     3301 agactctaca ataacaaggt tctttgccat aattttcagt aactctttgt gtatccataa
     3361 tcagggcgga cacggcggtc caccctacaa gttataacta tcaattgcca gattaagcca
     3421 acaaacgcgc ataaccccca ggaaccgagg taacaagccc cttcaattcc aattcgagta
     3481 gcatgataga tacagcctta gccgtcaatc cacttcgcat cacaaccaca tctacagaag
     3541 tagaatcata ccctacctga gataataacg cttcctccgt cacgctgaga tttttttcta
     3601 aagctggttt tttattcagt tcttctttgg agcgtatcac atatttcagt atttcaccca
     3661 attcttcgga gacatcttct gctgtttcga ctaacttagc tccctgtcga attaaagcat
     3721 gacatccttt tgctaatgga ttatggatag atcctggaat ggcaaaaact tctcgcccct
     3781 gatctaaagc cactttagcc gtaatcagtg atccgctatt taacgtcgct tcgatcacca
     3841 acacgccaac acttaaacca ctaataacgc gatttcgacg tggaaaatga gagtgcgcgg
     3901 gtggagtacc aatgggaaac tccgacacta aagctccccc tttttctaca atttcgcggg
     3961 ccagtgcgcg atggacagca gggtaaatgc tatcgagacc attacccaac accgcaatgg
     4021 tgttgcctac acccgacaaa gcacccttat gcgcagcgcc atcaacccca atggcaagac
     4081 cactcgtcac tgtaaagccg atacatgaaa aatggtgcgc aaatttcatc gcgttttcac
     4141 atccaatcgg cgttgggtta cgactcccta caatggcaat ttgcggcctg gccaataact
     4201 ttaggttgcc ctgcacaaat aacaccggag gtgccccgtg tgtttcttta agcaagggtg
     4261 gataaagtgc atcatattgc gtgatgatat gacagtcttt accactcatc caggcgagat
     4321 cttgatcaac acgagaccaa tctgcaattc ccttgataac tttgcagttc cctgctgcat
     4381 caaaggcctc cgctagatct gtgtattttt taagtatttc ggataacttc acagggccca
     4441 tgccggagat ccgcagcaaa gctaatagaa accgtcgttg ttctgtttta aaactcattg
     4501 tttttcttgc cgcttgttta gggactttta accttatcca ataaacttat catttccgtc
     4561 gattctagta tcaatcctaa actcaatttc tcatatgttt gaaacaccaa caagctccca
     4621 atgcggagat ccggcaattg cacaaaacat tttcgccatc ctttagaagt ggggtcgaca
     4681 attttccgac cattttgcat aatgtctaag gtatcacctg ctacaagtcc ttctcgttgt
     4741 cccagactaa taagaaccac ttgattacgc cccgcttgat taatcccgtc tcgaacagaa
     4801 agaataaagc cttctgctgc cataggcttc gccggatgaa tcgttagcgt gctcggcaac
     4861 gatgatgcaa cgcttggtaa tagccttgaa ccaacttcaa tgctttctgt cgcagtcact
     4921 acatcaaata ccgcgggatc tccaggctta attaattctg cgctaccaat actaatcgcc
     4981 tcaaacccaa gtttttcgcc tgtacaagga tgcttatagc ttcgacccgc gcgataaatg
     5041 ccataagcag agtcttctcg gcatttcgtt cccaaaacat ataacactgt tccaggacca
     5101 ccttcaatcc gatttttatc ctccgctatc acagaaggag cctttaaaaa ttgtttttta
     5161 tcctccaaaa taacactcat tttgataaaa ggtcgcagtg tttccaacga gagcgtggtc
     5221 acgggtttaa taatcttcga ttctcgaact ttcggtttta attttacggt gtttaatttg
     5281 tcatttgtcg tacctgtacc tgcataggct atacagctta ttaaaagcag tgtcaccacc
     5341 ccacaacctg ctctaagcat catgcacgct ttcctccaaa aatatttatt cgagaatatg
     5401 ttatagagta tagtgggatt ttgcggcact attaaattag ttgtaaaatt aactttttaa
     5461 aaggtacaac catgtcactg ttaaacatat tacagttccc agatccccga ctaaaaaaaa
     5521 ttgccacacc cgttgctgtt tttgatgacg agctcctgtc ccttatcact aatatgttcg
     5581 aaaccatgta tgaagcccaa ggcgtaggac tcgcagccac gcaagtgaat gtccataaac
     5641 gtgtgattgt gcttgatgtc tctaacgaac aaaatcaacc tctcgcactg attaatcctg
     5701 aaatgattac caaggtcggc atacttgaaa gggaagaggg ctgcctttct tttcccggcg
     5761 tttacgccaa agtaaaacgc taccaagaca ttgaaataaa atatcgtgac cgtaacggaa
     5821 aattgcaaaa ccttaaagcc aatggtggat tgttaagcgc ttgtatacaa catgaaatcg
     5881 atcatttaga tggcattact ttttttgatc atctctcttt attaaagcga gaattgttag
     5941 aaaaaaaatt agacaaaaat cgaaaacgcg cattatgaga atcgtatttg ccggcacgcc
     6001 agaatttgct gttcaacccc ttaaagcgtt actcgactcc aaacattctg tcatcgctgt
     6061 ttttacacag cctgatagac ctgcgggacg cggacaaact ttacatgcaa gcccagttaa
     6121 agcattagca ctctcgcacc atattcccat ttatcaaccg atccttttaa aaaaccctga
     6181 agaacaggca aagctccacg cgcttcaacc cgatctgctt atcgttgtgg cttatggact
     6241 cctattgccc aaagctgttt tagacattcc aaaatttggc tgtattaata ttcacgcgtc
     6301 tttgctgccg cgctggcggg gtgctgcgcc tatccagcat gcaatattag caggagataa
     6361 aacaacagga atttgcacca tgcaaatgga cgaaggattg gataccggcg atgtgctttt
     6421 aaagatatcc tgtactattg cacctcaaga aacaagcaaa acactccatg ataaattggc
     6481 aacgctagga gcagccgctc tactcgaaac actcgaaaaa ttcgaacaca atgcgcttgt
     6541 tccggaaaaa caagatccaa caacagcaac atatgctaca aaaatttcta aaacagacgc
     6601 taaaattgat tggcaagaaa gtgcggatgt catcgatcgc aaaatccgtg cgtttaaccc
     6661 ttggcctgtt gcatttgcgg aattatccgg acaaaccatt cggatctggt ccgcaaaact
     6721 gaatttaact gcaaccgtca ataataaaat aaaacctggc actattatcg aaattcataa
     6781 agacagcatg gatgtcgcaa caggcgacgg cgtactcacg ttattagaaa ttcaattgcc
     6841 cggcggtaag cgtttacctg tcgcagagat attgcatgca aagaaggcgc tgtttcaggg
     6901 ctaatgcgat aatgataagc ttactatatt tagctcaaca atgatgtttt ttaaggtatt
     6961 tttccattct caagcgcggt tattgcattt tcctgctcta cagtg
//
LOCUS       DAORVT010000015         6947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_6684_length_6947_cov_5.223157, whole genome shotgun sequence.
ACCESSION   DAORVT010000015 DAORVT010000000
VERSION     DAORVT010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6947
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6684_length_6947_cov_5.223157"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="PK389_00625"
     CDS             complement(<1..450)
                     /locus_tag="PK389_00625"
                     /inference="COORDINATES: protein motif:HMM:NF013235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HQY22248.1"
                     /translation="MSKKLIAMDNELIRSSYVLDVSELKLIYTALSKLDSSKQIEPRT
                     AIYITKNDYVQLGTNPKNIVREVRYACEKLMKRTLVINTEIGDLYTHWMHHVWLLKSD
                     DLTDLMNNYEDAKKLLRPNIAHQKMVLDILKELKTCDENLVARIEFHD"
     gene            complement(1210..1911)
                     /locus_tag="PK389_00630"
     CDS             complement(1210..1911)
                     /locus_tag="PK389_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002058850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HQY22249.1"
                     /translation="MRHSYHIEKHYIERSGWLRAAVLGANDGIISVTSLVVGIAASGA
                     SSQTLLVTCVAGLISGAASMAAGEYISVKSQQDIEKNDLKMEARELKLHPEHELQELK
                     NIYIQRGLEPTLAEDVAKQLTMHNALDAHARDEIGISDHTSAKPFLAASSSALAFSVG
                     SLFPLISMMLLPEQYLDKGVMLIGVLTLGIMGALASYAGGVSIWRGSVRVMIWGIVAM
                     LFSAWIGSLFNVAVT"
     gene            2256..2873
                     /locus_tag="PK389_00635"
     CDS             2256..2873
                     /locus_tag="PK389_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HQY22250.1"
                     /translation="MAQNTATSSTDLLIRFLHTVIILSFTGAYLTGDAEEWHQVHMAF
                     GYTLGISLILRILWQFLASRFAHTQPSGASRRFNVIKPFVQRYWAQPQQWLSPAFLKA
                     ASSSLFQLSILGVFCLMPLTVLAGFLTDYTHSHSLKEVHELFANLFLATVLLHLTALT
                     LNSVLLKKWLAKRMFWGTQSHSWFTLFASVLSLGVLVAFWFWYFS"
     gene            3031..3324
                     /locus_tag="PK389_00640"
     CDS             3031..3324
                     /locus_tag="PK389_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004973743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HQY22251.1"
                     /translation="MSAVNFNMRLEAELKEQVTPILEDYGLTMPQAFKLFLNQIVKTK
                     AIPLSFDYARETALTPKAAAKLLQSLKEIENGEYTEYETVEEAIKAMSEEAHG"
     gene            3317..3598
                     /locus_tag="PK389_00645"
     CDS             3317..3598
                     /locus_tag="PK389_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HQY22252.1"
                     /translation="MAKRKIIVTSSFKRDIKRRYLELVTAEWAEVLDCLVANNSLPEK
                     YLDHPLKGGAEFKNCRDCHVKPDLVLIYRLVGDDILELHQLDSHSEIFG"
     gene            complement(3710..4210)
                     /locus_tag="PK389_00650"
     CDS             complement(3710..4210)
                     /locus_tag="PK389_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004969149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22253.1"
                     /translation="MSNQNDLDDQLYILLASMKEYREAIADDKKRLEQFYNHVASGVL
                     DKAEKSLENANKKHTGALNNSIQALNEATHRLNLKLIIIFASTFVAVMMVFILAIFLY
                     IPSKDEIDARRADMAVLKKYPLQIEEHDGKTLVRIMTKKPCYSFEPNNVKEKTYDWCQ
                     IDPKKY"
     gene            complement(4207..5178)
                     /gene="mobQ"
                     /locus_tag="PK389_00655"
     CDS             complement(4207..5178)
                     /gene="mobQ"
                     /locus_tag="PK389_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005265044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HQY22254.1"
                     /translation="MAIYHCSTKTVNRSSGRTAVASSAYRAGEKLEDERTGLTHDFTR
                     KDGVAHSEIISNLDIEIDRGELWNLAEKTENRKDARTAREWVIALPDELDADQRKDLA
                     KDFARSLVDRYDVIADLAIHEPSKGGNDKNHHAHIMLTTRKAELDTDNKITLTTKTDI
                     ELSNAKRKSLGMGTTQEDIKQIRETWADLANKALERAGYREKIDHRSYADQNNGLQAT
                     IHEGTKVTQLRRQGIDTEISRFNDNVKQQNTQQLDQQKQQKESVLQRGLNRVDQGFDQ
                     WQKNQESKRLELERQAEMKRQQELDKQRAEQALRKVSQKLSRGGMSL"
     gene            5388..5672
                     /locus_tag="PK389_00660"
     CDS             5388..5672
                     /locus_tag="PK389_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HQY22255.1"
                     /translation="MTKSAENIEKKIEAQLEKLKQLKAQKQAIEARERTKKKEQERKD
                     DTRRKILLGSYLIKKMQANEANKEKILAELNEYLTENRDRQLFDLPDIEA"
     gene            complement(5669..5965)
                     /locus_tag="PK389_00665"
     CDS             complement(5669..5965)
                     /locus_tag="PK389_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004856442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22256.1"
                     /translation="MIKLNNLSTDLKHVTVEYLDIVNYAIARENICGYIFLLSRLSKN
                     AEPTEKMQMESKIQDLIYYRDNLQIEDKDNIQKVLNTLIPEYQAEQNNQTAKKN"
     gene            complement(5962..6891)
                     /gene="repM"
                     /locus_tag="PK389_00670"
     CDS             complement(5962..6891)
                     /gene="repM"
                     /locus_tag="PK389_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="HQY22257.1"
                     /translation="MKNSLVVKDNALINASYNLELTEQRLIMLAIINARESGQGITAD
                     SKLEIHASDYAKLFNVSADASYKALREAVNNLFNRQFSYTAEYKKTGKVGIVRSRWVS
                     RIFYVDDLALLEITFAPDVVPLITRLEEHFTKYEAKQVAHLTSKYATRLYELLIAWRE
                     VGKVPQLELSEFRNRLGLVDSEYTAMSDFKKRVLEPSIKQINEHTDITVTYEQHKKGR
                     IISGFSFKFKQKQQPKVEAKRDPNTPDFFIKMTDAQRHLFANKMSEMPEMGKYSQGTE
                     SYQQFAIRIADMLLEPEKFRELYPILEKSGFQP"
BASE COUNT         1988 a         1312 c         1306 g         2341 t
ORIGIN      
        1 atcatgaaat tcaattctag ctacgagatt ttcatcacaa gtttttagct cttttaatat
       61 atctaaaacc attttttggt gagcaatgtt cggtcttaat aatttttttg catcctcata
      121 attattcatg agatctgtta agtcatctga tttcaataac cacacatgat gcatccagtg
      181 tgtgtaaagg tcacctattt cggtgtttat aactaatgtt cttttcatta gtttttcaca
      241 cgcatagcgc acttctctta ctatattctt tggatttgta cctagttgta catagtcatt
      301 tttagtaatg taaatagccg ttcgaggttc aatttgtttt gagctatcta attttgatag
      361 tgctgtataa attaacttca attcactgac atctagcaca taagaagatc gaattaactc
      421 gttatccatc gctattagtt tttttgacat ttttttcaat taaatgatcg tgataacaat
      481 acttttacct ataaaaggga gtgaagtcaa tatttctccc atctgtgcgt catgttctcc
      541 acctctgtgc gtcatgttct ccacctctgt gcgtcatgtt ctccacctat atcctataaa
      601 accttctgat agcaatggtt ctggatggtc taaaaagctt ttaaaaagat ttaaaaagat
      661 aaaaaggaaa atcaagaaaa aatcaaaggc tatggcactc gcttcgctcg tgtcgtttca
      721 aaagcaaaag ctccaaagtt cgcacccttc gggatgctct gtatctttcg ttattaagtt
      781 aaggggagct tgtaatcagg gaaaaggcag ggacgaaatt tttatgatgc tcaatcgctc
      841 gtagacactc gctcggttct tactggaatg attaaaatat ctaatcaaaa ccgtggaaca
      901 atgatcaaat agtaagcgtg gaacgtgata aatcataaaa aaagccccga ttggagaaaa
      961 ggggctttta aggcttcact atatcattga aaattaaaga aaagatatag gcatttcgta
     1021 taaggtgtat tatgttaatt ttaaaaatct ttgaagcaga tgattgagta tcttagtttt
     1081 gacctctaat caggttagat catatgtaat ataagtatct aaaagttagg agaactcatg
     1141 aataataact actcaaaacc tataataatt tattggatta aaatattgtg atcagataca
     1201 caaagtatat taggtcacag caacattaaa tagtgatccg atccaagcac tgaataacat
     1261 tgcgacaatt ccccaaatca taacccgaac agaacctctc cagatactta caccgccagc
     1321 ataactcgcc aatgctccca taattccgag agttaaaacg ccaatcagca tgacaccttt
     1381 atctaaatat tgttcaggca gaagcatcat tgaaattaaa ggaaataatg aacctacaga
     1441 aaatgcgagg gctgaagaag atgccgctag aaatggcttg gcagaggtat gatctgaaat
     1501 gccaatttca tcacgagcat gtgcatctaa agcattatgc atagttagtt gtttagcgac
     1561 atcttcagct aaagttggtt ctaaaccacg ttgtatataa atatttttta gttcttgcag
     1621 ttcatgttca ggatggagtt ttaactcacg tgcctccatt ttcagatcat ttttttcaat
     1681 atcttgttgt gatttaacgg aaatatattc acctgcagcc attgaagccg ccccagaaat
     1741 taatcccgca acacatgtta ctaataatgt ttgagaagaa gcaccactgg ctgctattcc
     1801 gactactagg cttgttacag aaataatccc atcattagcc cctagtacag ccgcacgtag
     1861 ccaccctgac cgttcaatat aatgtttttc gatatgataa gaatggcgca caatatagtc
     1921 ccttgctgtt atcagaatga ttatagtaaa tattttatct gtaaacctta attgagctag
     1981 acaatagaga atataagtca ctactgatgc atatccatta catttaaaat aaccttgttt
     2041 aataatgggt gttatacgta ttaaaaaaac cgactccaat gttcaggctt cgtataatcc
     2101 atattaagct aatggtagac aaaattaata ttcacatgat cttccaattt taaaattgcg
     2161 ctctaatgag cgcaattttt ttgactgtat ttttaatatt aacctcagct tgagctgcta
     2221 ttctgcaact aaaatacagt caacaaaggt tttttatggc tcagaacaca gcaacatctt
     2281 ctacagattt actgattcga tttctgcata ccgtcattat tttgagcttt actggggctt
     2341 atctcacggg ggatgcagaa gaatggcatc aagtgcatat ggcgtttggc tatacacttg
     2401 gtattagcct gatattaaga attttatggc agtttcttgc ttctcgattt gctcatactc
     2461 agccttcagg ggcgagccgt cgatttaatg tgatcaagcc atttgtgcag cgctattggg
     2521 cgcaaccgca gcaatggctt tccccagcat ttttaaaagc agccagttcc agcctgtttc
     2581 agctgagcat actgggtgtt ttttgcctga tgcccttaac tgtactggca ggctttttaa
     2641 cagactatac gcatagccac agtttaaaag aagtgcatga actattcgca aatctgtttt
     2701 tagctactgt attactacac ttgacagcac tgacactgaa tagtgttcta cttaaaaaat
     2761 ggctagcaaa gcgtatgttt tgggggacac aatcacattc atggttcacc ctgtttgctt
     2821 cagtcctttc actaggtgtg ttggtcgctt tctggttttg gtattttagc taagctttta
     2881 tgagagcaac acagatgaag ctctatttaa cataatggct gttatacgaa attcatttgt
     2941 tagaaaccat ttattacatc aaaagtaatg aaaaagtaaa gaatatgcct tatattatct
     3001 ttactttaat ggtttttgag tgaggaaatc atgtccgcag tcaatttcaa tatgcgctta
     3061 gaagcagagt taaaagaaca agtcacgcct attttggaag actacggttt gactatgccc
     3121 caagcattca aactgttctt gaatcaaatc gttaaaacta aggcgatccc tttatctttt
     3181 gactatgcgc gagaaactgc tttaactcct aaagcagcag ccaagttact tcaatcttta
     3241 aaagagattg aaaatggcga gtacaccgag tacgaaacgg tagaagaagc gattaaagct
     3301 atgtcggaag aagcacatgg ctaaacgtaa gattatcgta accagttctt ttaaacgtga
     3361 tattaaaaga cgttatttag aactggttac ggctgaatgg gcagaagtat tagattgttt
     3421 agttgcaaat aattctttgc ctgaaaaata tttagatcac cccttgaagg gtggtgcaga
     3481 gtttaagaat tgtcgtgatt gtcatgtgaa gcctgattta gtcttgattt ataggcttgt
     3541 tggtgacgat attttagaac tgcatcagtt ggatagccat tccgaaattt ttggatgact
     3601 atcgctcgat gctttgaaag atggctttag ccatgtttca aagtcgggca actttgcgct
     3661 tgctgtaatc tgatcagtaa tgagaaaaac caagcaaagt accgatcaac tagtattttt
     3721 tagggtcgat ctgacaccag tcatacgttt tttctttgac attattcggt tcaaatgagt
     3781 aacacggctt tttagtcata attctgacca atgttttccc atcatgctcc tcgatttgta
     3841 acggatattt ttttagtaca gccatatctg ctctacgtgc atcaatttcg tctttgcttg
     3901 gtatgtataa gaagatagcc aaaatgaata ccatcatcac agcaacaaac gtgctagcaa
     3961 aaataatgat aagttttaag ttgagcctgt gtgtagcttc gtttaatgcc tgaatgctgt
     4021 tgtttaatgc gcctgtatgc ttcttatttg cgttttctag cgatttttca gccttgtcta
     4081 gcactccact agcgacatgg ttgtaaaact gctctaatcg cttcttatcg tctgctatgg
     4141 cttctcgata ttccttcatg gatgccaata aaatatataa ctggtcatct agatcatttt
     4201 gattactcat aatgacattc cgcctctact tagtttttga gataccttgc ggagtgcttg
     4261 ctcggctcgt tgtttatcta attcttgctg tcgcttcatt tccgcttggc gttccagttc
     4321 tagacgtttg ctttcttggt tcttctgcca ttgatcaaaa ccttgatcga cacggtttaa
     4381 accacgctgt aaaacgctct ctttctgctg tttctgttgg tcaagttgtt gggtattctg
     4441 ctgtttcaca ttgtcattga aacggctaat ttcagtgtct atgccttgtc tgcgtaattg
     4501 agtgactttg gtgccttcat ggatggtggc ttgtagtccg ttgttctgat cggcatagct
     4561 gcggtgatct attttttctc ggtagcctgc acgttccaat gctttattgg ctaagtctgc
     4621 ccatgtttct cgaatttgct taatatcttc ttgggttgtc cccatgccaa ggctttttcg
     4681 tttggcgttg ctgagttcaa tatcggtttt ggtggtcagg gtgattttat tgtccgtgtc
     4741 tagttctgcc tttcgggtgg tgagcatgat atgggcgtgg tgatttttat cattaccgcc
     4801 tttactcggt tcatggattg ctaaatctgc gattacgtca tagcgatcaa ctaaggacct
     4861 ggcaaagtct tttgccaagt ccttacgttg atcagcgtct aattcatcag gcagggcaat
     4921 cacccattct ctagcggttc gggcatcttt gcggttctcg gttttttctg ccaagttcca
     4981 aagttcacca cggtcaattt caatatctaa attagagata atttcagaat gggcgacacc
     5041 gtcttttctc gtaaagtcat gggtcagccc tgtgcgctca tcttctagtt tttcacctgc
     5101 acgataggca cttgatgcaa ccgcagttcg tcctgaactt cggttaactg ttttcgttga
     5161 acaatgatag attgccatgc agtcgccttg agatgctttt gcttttaaaa aattgcgtag
     5221 cagttttttg gggttaaggg gatacccctt acgcaaactt tgacttggat gaaatccaag
     5281 tcgtaagtgc gcatttcatg aaaaacacga tagcatatta cccgcttgaa tttcaattca
     5341 taggatctta tgcttgagtg gttttctata ttccgaaatt aaccgttatg actaaatctg
     5401 ctgaaaatat cgaaaagaaa attgaagccc agttagagaa gctgaaacag ttaaaagcac
     5461 agaaacaggc tattgaagca agagaacgta ccaaaaagaa agaacaggag cgcaaggatg
     5521 atactcggcg taaaattttg ctcggttctt acttgattaa gaaaatgcag gcaaatgaag
     5581 ccaataaaga aaaaatactc gctgaactca acgaatattt aacagaaaat agagatcgac
     5641 agctttttga tttacccgac attgaagcct aatttttctt cgctgtttga ttgttttgtt
     5701 cggcttgata ctctggaatg agcgtattta aaaccttttg aatattgtcc ttgtcttcta
     5761 tctgtaaatt atctcgatag taaattaagt cctgtatttt gctttccatc tgcatttttt
     5821 cggtgggttc ggcatttttg gatagtcggg ataataggaa gatataacca catatatttt
     5881 ccctcgcaat tgcatagttt acgatgtcga ggtattcaac ggtcacatgt tttagatcgg
     5941 ttgataggtt atttaatttg atcatggctg aaatcctgat ttttctaata ttggataaag
     6001 ctctctgaat ttttcaggct ctaaaagcat atcagcgata cgaatagcaa attgttggta
     6061 gctttctgta ccttgtgaat atttccccat ttcaggcatt tctgacattt tattggcaaa
     6121 taaatggcgt tgtgcatcgg tcatttttat aaaaaaatca ggcgtgtttg gatctctttt
     6181 tgcttcaact tttggctgtt gtttttgctt aaatttgaaa gaaaaacctg aaatgatccg
     6241 tcctttttta tgctgttcat acgttacagt aatatcagta tgctcattaa tttgtttaat
     6301 tgatggttca agcacacgct ttttaaagtc actcatggcg gtgtattcac tatcaacaag
     6361 acctaatctg tttctaaact cactaagttc tagttgtgga acttttccaa cttcacgcca
     6421 agcaatcagc aattcataaa gcctagtggc gtatttactg gttaggtgag caacttgttt
     6481 agcttcatat tttgtaaagt gttcttccaa tcgtgtgatt aaaggcacta catcaggagc
     6541 aaaagtaatt tctaacaatg ctaggtcatc tacataaaat atacggctta cccagcgaga
     6601 tcgaacaatg ccgactttgc ctgttttttt atattcggct gtataactaa attgacgatt
     6661 aaacagatta tttacagctt ctctcaaagc tttatatgac gcatcggcag atacattgaa
     6721 tagcttggcg tagtcactag catgtatttc cagtttacta tccgcagtga ttccttgtcc
     6781 cgattctcta gcattaatga tagctagcat gatcaaccgt tgttccgtta gctctaggtt
     6841 gtaactggca tttattagag cattatcttt cacgactaaa ctatttttca taagaagaac
     6901 ttattattat ctaattaaag gacaatgtaa tctaatatct ttttaag
//
LOCUS       DAORVT010000016         6516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_7494_length_6516_cov_3.427952, whole genome shotgun sequence.
ACCESSION   DAORVT010000016 DAORVT010000000
VERSION     DAORVT010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6516
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7494_length_6516_cov_3.427952"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="PK389_00675"
     CDS             complement(<1..510)
                     /locus_tag="PK389_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22258.1"
                     /translation="MKNQLGVTIQTLTNLSKKQLVSVKRFVSECKKSDGYMPIFYWSS
                     IETRKNPGINEILCYAENEKLAAYVALYHFDAHEIEITLMIDPAYRNTAFYSLLWEQI
                     KQATTQCPVLITRFVFTLNQASSSFKEFLQGLGACAFDITYKLALTAGKFSKIKAVDD
                     CGLTFRKAVR"
     gene            complement(910..2355)
                     /gene="lpdA"
                     /locus_tag="PK389_00680"
     CDS             complement(910..2355)
                     /gene="lpdA"
                     /locus_tag="PK389_00680"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HQY22259.1"
                     /translation="MDANQKVDVVVLGSGPGGYTAAFRAADLGKKVVLIEREESLGGV
                     CLNVGCIPSKALLHAAKVISEAAEFKEHGIRFGEPAIDLNALRTWKESIINRLTSGLK
                     GLAKQRKVTVIRGEGYFLSANQIVVKNSEGDQIVSFENAIIAVGSRSVQLPFLPNDPR
                     IINSTGALALKEIPKRLLVIGGGIIGMEMATVYHALGAKITVVELMDGLLAGVDKDIA
                     APLYKRTQKQYENILLETKVTKVDATPEGLFVTFEGKNAPKAPERFDIILCAVGRVPN
                     GKNISAEKAGVRVDERGFIPVDKQQRTNIPHIFAIGDVVGNPMLAHKAMPEGRVAAEV
                     IAGKKRFFEPNCIPSVAYTDPEVAWAGVTETEAKAKNIAYEKGVFPWMASGRSLSLGR
                     DEGITKLLFDKETHRIIGAGITGPNAGDLISEIVLAIEMGCDAEDIALTIHPHPTLSE
                     TICLSAEAFEGTITDLYLPKKELQKSSDTNQ"
     gene            complement(2373..3647)
                     /gene="aceF"
                     /locus_tag="PK389_00685"
     CDS             complement(2373..3647)
                     /gene="aceF"
                     /locus_tag="PK389_00685"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HQY22260.1"
                     /translation="MLKEVLVPDIGSYSNVDIIEISVSIGDVIKADETLMTLETEKAT
                     LEIPAPFAGIVKAVHVKVGDKVSEGSLIVSIEGVEEAVPSSSHIPPSSPKIPPNPPLP
                     KGGITTENIPPSPPVKGEIVHAGPGVRRFARELGADLSRIGGTGPKNRILKADLQAYV
                     KRMLSQIQSGNTSPGGGGISVAAWPQVDFAKFGEISKEPLSRIKKLSGSFLHRNWVMV
                     PHITQFDEADITELEQFRKAQQSLAEAQGVKLTPLIFIMKAVVATLKEFPWFNASLDA
                     NGAELILKKYFHVGVAVDTPNGLVVPVVRDVDQKGLFQLAKELGEISQKARSGQLTAQ
                     EMQGSSFTISSLGGIGGTAFTPIINVPDVAILGVSKAQIKPVYDESGEFKPRLLLPLS
                     LSYDHRVIDGALGARFITFLVKQLSDIRRLLL"
     gene            complement(3667..6345)
                     /gene="aceE"
                     /locus_tag="PK389_00690"
     CDS             complement(3667..6345)
                     /gene="aceE"
                     /locus_tag="PK389_00690"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012800646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HQY22261.1"
                     /translation="MRDDHNFDDIDSIETQEWLDALASVLAAAGKERAQFLLKELTTK
                     ALSMGCSVTEGFQSPYINTISADQEAKIPGDAVMERRIRSLIRWNAVAMVVRAGKGKL
                     DPDLGGHIGTFASSATLYDVGFNHFWRAANEKQNGDLLYIQGHSSPGIYARSYLEGVL
                     SESQISNFRQEVDGIGISSYPHPWLMPEYWQFPTVSMGLGPMQAIFQARFMRYLEARG
                     LEKNTGRKVWCFCGDGEMDEPESLGSIGLAAREKLDNLVFVVNCNLQRLDGPVRGNGK
                     IIQELESVFKGAGWNVIKVIWGDLWDPLLAKDKSGLLLKRMEEVVDGEYQNYKAHGGA
                     YTRENFFGKYPELKELVADMSDDDIWRLNRGGHDAQKVYAAYKTASECVGKPTVILAK
                     TVKGYGLGEAGEARNIAHNLKKMTLEQLKQFKDRFDIPIPDDKIADVPFYRPSEDSPL
                     IQYMLAQRKALGGFLPHRRTEADETLEIPPLSIFEGLLKGTGERKISTTTVFVRLLTA
                     LCKDKTVGQRVVPIVPDEARTFGMEGLFRQLGIYSPVGQLYQPVDSEQVMYYKESKDG
                     QILEEGLNEAGSFCSWLAAATAYSTHNKIMLPFYIFYSMFGFQRIGDFAWAAGDSRAR
                     GFLIGGTAGRTTLNGEGLQHEDGHSHILANTIPNCISYDPTFSYELAVIIHNGLHRMV
                     TLKESVFYYITTMNENYEHPDMPEAVEAGILKGLYRFKKSEKPASKQRVQLLGSGTIL
                     REVIEAAKLLEKDFDVAADIWSAPSFNLLGRDGLEADRWNRLHPLETPKVPYITSCLA
                     ETAGPVIAATDYIKQYAEQVRAFIPRAYIVLGTDGFGRSDTRQQLRRFFEVDRYYIAV
                     SALKALADEGLIAKELVVKAMQQYQINPDKPNPLSV"
     gene            complement(6338..>6516)
                     /locus_tag="PK389_00695"
     CDS             complement(6338..>6516)
                     /locus_tag="PK389_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018678350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HQY22262.1"
                     /translation="LADLVVLLKKTYPEIKDSRIAGHSTIAPMRKTDPGSVFDWNLLA
                     ALSMKTEYSEENNA"
BASE COUNT         1902 a         1576 c         1133 g         1905 t
ORIGIN      
        1 tcgcaccgct tttcgaaagg ttaagccaca atcatcaacc gctttaattt tagaaaattt
       61 tccggctgtt aacgctaatt tatacgtaat atcaaatgca caggcaccca acccttgcaa
      121 gaattcttta aaggaagaag acgcctgatt taatgtaaaa acaaagcgcg taatcaaaac
      181 aggacattgt gttgtcgctt gttttatttg ttcccacagt aaactataaa atgctgtgtt
      241 tctatacgcg ggatcaatca ttaaagtaat ttctatttca tgagcgtcaa aatgatacaa
      301 cgctacataa gcagccaatt tctcgttttc tgcataacac aagatctcat taattcctgg
      361 gttcttcctg gtctcaatac tgctccagta aaaaatgggc atatagccgt cagacttttt
      421 gcattccgat acaaatcgct taacgcttac cagctgtttt ttagacagat ttgtaagcgt
      481 ctgaattgtt accccaagct gatttttcat acagttccat atctgacttt acttgaatcg
      541 tagcgcgatt ataccctaat gaggcatggc agggtcaagt gctagcgcac cactcgttat
      601 ttttaagggt gcttgccttg ctatccctag atatagaatt ccagccctcg aaccaacctc
      661 agcaagtgac taaataaagg tcatcaatga acgaacgcaa taaccttgta gacaatctag
      721 ttgagattac taatcttgta ttaaaaaagt acaaaaatat ctcgtggaaa caagaccaaa
      781 aatacaaccg gtgcgaagtt tgtttgcttg atccctgtaa aagaagcctt gttacaaaag
      841 aaactataat acaatatcct agcgctgtac tgcccctctc aaaaaacatt caagcatgtc
      901 tcaaccaatt tattgattag tatccgaaga tttttgcagt tcctttttag gcaaatagag
      961 atcagtaata gttccttcaa atgcctctgc agataaacaa atagtttccg acaatgtagg
     1021 atgcggatga atcgtcaagg caatatcttc ggcatcacat cccatctcaa tagcgagcac
     1081 tatctccgaa atcaaatcgc ctgcattcgg acccgttatt ccagcaccaa taatgcgatg
     1141 cgtttcttta tcgaacaaca acttagtgat cccctcgtca cgccccaaac ttaaagagcg
     1201 accactggcc atccagggaa acacgccttt ttcgtaagcg atatttttgg ccttggcttc
     1261 tgtttctgta acacccgccc acgcgacctc aggatctgtg taggcaacag atggaataca
     1321 attgggttca aaaaagcgtt tttttcctgc aataacttca gcagccactc tcccctctgg
     1381 catggcttta tgcgccaaca taggattgcc tacaacatcg ccgatcgcaa aaatatgcgg
     1441 aatattcgtg cgctgttgtt tatccacagg aataaaacca cgctcatcta cgcgaacacc
     1501 cgctttttca gcactgatat ttttaccatt tggcactcgc cccacggcac ataaaataat
     1561 atcaaatcgt tcgggtgctt ttggcgcatt tttgccttca aaggttacga acaagccttc
     1621 gggagttgca tcaacctttg tcactttagt ttctaacaaa atattttcat attgcttttg
     1681 agttcgctta tacaatggcg ccgctatatc tttatccaca ccggctaata aaccatccat
     1741 cagttccaca acggtaattt tagcgcctag cgcatgataa accgttgcca tttccatacc
     1801 gataattcca ccaccgataa ccaataatcg tttaggaatt tcttttaagg caagcgcgcc
     1861 tgttgaattt ataatacgtg gatcatttgg caaaaatggc aattggacag aacgcgaacc
     1921 cacagcaata atcgcatttt caaatgaaac aatttgatca ccctcgctgt ttttaacgac
     1981 gatctgattg gcagaaagaa aatagccttc gcctcgaatg acagtgactt tgcgttgttt
     2041 ggccaagccc tttaacccac tggttaatcg gttaatgatg ctttctttcc aggttcttaa
     2101 tgcatttaaa tcgattgcgg gttcaccaaa acgaattccg tgttctttaa attctgctgc
     2161 ttctgaaatg actttagccg catgtaacaa cgctttagaa gggatgcaac ctacatttaa
     2221 acaaacgcca cccaaacttt cttcacgttc aattaaaacc acctttttac cgagatcggc
     2281 tgcacgaaac gcagcagtat agccaccggg gcctgagccc aaaaccacta catcgacttt
     2341 ttgatttgca tccatagaca taacattcct ccttataata acagccgacg aatatccgat
     2401 aattgtttca ctaaaaatgt tataaaacgg gcccctaaag ccccatcaat aacgcgatga
     2461 tcatacgaca aagacaaagg taataagaga cggggtttga attctcctga ttcatcataa
     2521 acgggtttta tttgggcctt tgaaacgcct aaaatagcaa catcaggtac attaataata
     2581 ggcgtaaatg ctgttccacc aattccacct aagctggaaa tcgtaaaaga actgccttgc
     2641 atttcttgcg cagttaattg accactgcgg gctttttggc ttatctctcc caattctttc
     2701 gctaattgaa acaagccctt ttgatccaca tcgcgcacca cgggcacaac taaaccattc
     2761 ggcgtatcga cagcaacgcc aacatgaaaa tattttttta aaatcaactc agcaccattt
     2821 gcatccaaag atgcattaaa ccaaggaaat tcctttaacg ttgcgacaac tgccttcata
     2881 ataaaaatca agggtgttaa tttaacaccc tgcgcttccg ctaacgattg ttgtgcctta
     2941 cgaaactgct ctaattctgt aatatctgcc tcatcaaatt gtgtaatatg cggtaccatt
     3001 acccaattgc gatgcaaaaa agaacccgat aattttttaa tacgcgacaa tggctctttg
     3061 gaaatttcac caaactttgc gaagtctact tgcggccatg ctgcaaccga tattccacca
     3121 ccaccaggag aggtgttccc agattggatc tgagacagca tgcgtttaac gtatgcttga
     3181 agatctgctt ttaaaatacg atttttagga cccgttccac cgattcgcga aagatcagcg
     3241 cctaattctc tggcaaaacg tctaacacct ggtcctgcat gtacaatttc tcccttcaca
     3301 ggggggctag gggggatgtt ttctgttgtt attcccccct ttggcaaagg ggggttaggg
     3361 gggattttag gagagctagg ggggatgtgg gaactagagg gaacggcttc ttccactcct
     3421 tctatactta caatcaaact gccctcagaa actttatcac ccactttaac atgtacagct
     3481 tttacgatac cggcaaaagg cgcgggtatt tctagcgttg ctttttctgt ttccaaagtc
     3541 attaatgttt catctgcttt aatcacatcg ccaattgaaa ccgatatttc aataatatcg
     3601 acattcgaat aagaaccgat gtctggaact aaaacttctt ttaacatata tttttaccct
     3661 atgattttag accgatagcg gatttggttt atccggattg atctgatatt gttgcatcgc
     3721 ttttacgact aattctttcg cgataagacc ttcgtctgcc aacgctttta aagccgaaac
     3781 tgcaatataa tagcgatcga cttcaaagaa acgacgcaac tgctgtcgag tatcacttcg
     3841 tccaaaaccg tctgtcccta acacaatata agcccgtggt ataaaggcac gaacctgctc
     3901 tgcatattgt ttaatataat cggttgcagc aatcacagga cccgcggtct ctgctaaaca
     3961 acttgtaata taaggcactt tcggcgtttc caatggatgc aatcgattcc aacgatcggc
     4021 ctccaagcca tcacgcccta aaagattaaa gctgggcgcg ctccaaatat ctgctgccac
     4081 atcaaaatct ttttctaata attttgctgc ttcaatgact tctcttaaaa tagtgcctga
     4141 acctaacaac tgaacacgtt gctttgatgc cggtttttcc gattttttaa aacgatagag
     4201 tcctttcaaa ataccggctt caacagcttc tggcatatcc ggatgttcgt aattttcatt
     4261 catggtggtt atataataaa aaacactttc tttcagcgtc accatgcgat gcagcccatt
     4321 atgaataatc accgccagct cataagaaaa agtcggatca taagaaatac aattgggaat
     4381 ggtattggcc agaatatggc tatgaccatc ctcgtgttgt aatccttctc cgtttaacgt
     4441 tgtacgccca gcagtaccgc caattaaaaa acctcttgca cgcgaatccc ctgcggccca
     4501 agcaaaatct ccgattcgct gaaaaccaaa catggaataa aaaatataaa aaggcagcat
     4561 aattttattg tgagtactat aagcagttgc tgccgctaac catgaacaaa aagatccggc
     4621 ttcgttaaga ccttcttcca aaatttgacc atctttggat tctttgtaat acatcacttg
     4681 ttctgaatcg accggctggt ataactgccc cacaggagaa taaatcccca attgacgaaa
     4741 caagccttcc ataccaaaag ttcgtgcttc gtctggcacg ataggaacca ctctttgtcc
     4801 aaccgtttta tctttgcaca aagccgttag caatcggaca aaaaccgtgg ttgtagaaat
     4861 ttttctctcg cctgtcccct tcaataatcc ctcaaaaatg gaaagtggtg ggatctctaa
     4921 ggtttcgtct gcttctgttc ggcgatgcgg caaaaatccg cctaacgctt ttcgctgtgc
     4981 caacatgtat tgtattaacg ggctatcttc actcggtcta taaaacggga catctgctat
     5041 tttgtcatct ggtataggaa tatcaaaacg atctttaaat tgttttaatt gttcaagtgt
     5101 catttttttc aaattatgcg caatatttcg tgcttcaccg gcttcaccta atccataacc
     5161 ttttacggtt ttggctaaaa taaccgttgg ttttcctacg cattcagaag cggttttata
     5221 agccgcatag actttttgtg catcgtgtcc accccgattt aaacgccaaa tatcatcatc
     5281 actcatatcg gcaacgagtt ctttcagttc tgggtatttt ccaaaaaagt tttctcgcgt
     5341 atacgcacca ccatgcgctt tatagttttg gtattcacca tctacaactt cttccatacg
     5401 ctttagaagc aatccacttt tatctttcgc taataacgga tcccacaagt caccccaaat
     5461 cactttaatg acattccaac ctgcgccttt aaaaacagat tctaattctt gaataatttt
     5521 tccgtttccg cgcacaggtc catctaatcg ctgtaaatta cagttcacga caaaaactaa
     5581 attgtctaat ttttcacgtg cagcaagtcc tatcgaaccc aaagattcag gttcatccat
     5641 ttcaccatca ccacaaaaac accatacttt gcgtcccgtg tttttttcca aacctctggc
     5701 ttctaaatat cgcataaatc gcgcttgaaa aatggcttgc ataggtccta atcccatcga
     5761 aaccgttgga aattgccaat attctggcat taaccaaggg tgcggataag aagaaatacc
     5821 aatgccatcc acttcttgtc tgaaattgct gatttgcgat tcagacaaca cgccttccaa
     5881 ataagatcgt gcatagatcc ccggggatga atggccctgt atataaagta aatcgccatt
     5941 ctgcttctca ttggccgctc gccaaaaatg attaaatccc acatcataca aggttgcgga
     6001 agaagcaaaa gtaccaatat gcccccccaa atccggatct aattttcctt ttccggcacg
     6061 cacaaccata gcaaccgcat tccaacgaat taaagagcga atacgccgtt ccatcacagc
     6121 atcgcccgga attttagctt cttgatccgc tgaaatcgta ttaatgtaag gcgactgaaa
     6181 tccttctgta accgagcatc ccatgctcaa tgcttttgtg gtgagttctt ttagcaaaaa
     6241 ctgtgcacgt tcttttcctg ctgctgccaa tacagaagct aatgcatcga gccattcttg
     6301 tgtctctatg ctatcaatat cgtcaaaatt atgatcatca cgcattattt tcctcactgt
     6361 actctgtctt catgctaagt gctgccaaca aattccaatc aaaaacagac ccaggatctg
     6421 tttttcgcat tggcgcgatt gtactatgtc ccgcaatccg actatctttt atctctggat
     6481 atgtcttttt taacaaaaca actaaatctg ctaaaa
//
LOCUS       DAORVT010000017         6336 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_7844_length_6336_cov_3.982487, whole genome shotgun sequence.
ACCESSION   DAORVT010000017 DAORVT010000000
VERSION     DAORVT010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6336
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7844_length_6336_cov_3.982487"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            362..1390
                     /locus_tag="PK389_00700"
     CDS             362..1390
                     /locus_tag="PK389_00700"
                     /inference="COORDINATES: protein motif:HMM:NF016822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HQY22263.1"
                     /translation="MSLNYVFRPIQESDMPAILEIAEQKGAGFNSLPNDAKVMQAKII
                     QSINSFSEKLDFNKRYYFFVLENSDTQEMMGSASIETNLGYLWPFYKYKISTLVQISK
                     ALNQHKEHQILSLVTEHQGATELGALYLKPAYRGEGRGTFLSRARCLFIAEFLNNFSE
                     KIVADMRGMSNQDEISPFWEAIGHYFADIPYSEASHLKATKGSQFLVDLMPHLPIYID
                     LLPEIAKKVIGKVHKNTAPALHVLEKEGFQFKNHVDIFDAGVTIEAEKLHLKTVRESK
                     KAALVAFKDSVKKEPTMMISNTKHNHFRAVVGALEELREKEIALSTAVAKQLQISVGD
                     KLRYCSLR"
     gene            1407..2873
                     /gene="astD"
                     /locus_tag="PK389_00705"
     CDS             1407..2873
                     /gene="astD"
                     /locus_tag="PK389_00705"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HQY22264.1"
                     /translation="MNGRNFIGGRWTEGTGPGFESENPATGEVIWRGYSADNRQINKT
                     VHFAKSAFQSWSALSLEKRMDYLERFCEELKKNRLLLSTTIALETGKPLWESDMEVGA
                     MINKLPISVKAYQERCRERVETLSSGVSITRHRPHGVIAVLGPFNFPGHLPNGHIIPA
                     LLAGNTVVFKSSELTPKVAEKIFFCWEQAKLPEGVINLIQGGRETGEILSKHPDLNGL
                     MFTGSFKTGHALANVFSEKAGKILALEMGGNNPLVVHEVSDIKAAALQVIQSAYITAG
                     QRCTCARRLIVTKGWNGQEFIEELIKMTNSLKIGPYTDQPEPFMGPLISNISANHVMT
                     AYDLLIHEGAKSLMPLKRIDENLPFLSPGLLDVSAIKVRTDEEIFGPLLQLTWVDDFE
                     AALIEANKTAYGLAAGLLCDNVEYYEYFRHKIKAGIINWNHALTGASSSAPFGGLGHS
                     GNHRPSAYYAADYCVYPVASLEENILKIPDNLPPGIQL"
     gene            2870..4207
                     /gene="astB"
                     /locus_tag="PK389_00710"
     CDS             2870..4207
                     /gene="astB"
                     /locus_tag="PK389_00710"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011919439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HQY22265.1"
                     /translation="MKTSEINFDGIIGLTHHYSGLAFGNLASKANAHKISNPKAAALQ
                     GLKKMHLLMQRGILQAVLPPHERPLLSFLKNLGFTGTDAKILKAVYALDPHLFGAACS
                     ASSMWVANAATVTPSSDSADCRVHITLANLMSNLHRALEAETNLKIFKKIFSDENYFK
                     IHPPLLSQVDFSDEGAANHNRLCQDYGSTGLNIFIYGRSGLQKKEILPKQYPARQTRE
                     ASKAVARLHQLRPENTFFIKQNPMAIDQGVFHNDVISVANQNVFLFHELAFTDMPELV
                     DLLKKKSQDSLYLLPVATADLSLQDAVKSYIFNSQLITLDTGVMALIAPIESRENKNA
                     HQVLLKIMAGKNPISELHFVDCRQSMLNGGGPACLRLRVVLTEAEQKACSARVFLDEA
                     LYQRLVQWIERHYRDRLSLGDLVDPRLLNEIYVALDELTQILKLGTLYNFQKI"
     gene            4257..5204
                     /gene="ubiA"
                     /locus_tag="PK389_00715"
     CDS             4257..5204
                     /gene="ubiA"
                     /locus_tag="PK389_00715"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HQY22266.1"
                     /translation="MFKRSNFSSESKALSEKRVALISFGKSIVKNQKIIFFIRLMRLD
                     KPIGIFLLLWPMLIALWVAGKGKPNPTIVCIFILGAIIMRSAGCVINDLFDRRFDISV
                     ERTKNRPLAVGAISIKEALFVFFTLIFFGFLLVLQLNILTIMMSIIALLLAIVYPLMK
                     RYTHLAQVVLGAAFGWAVPMAFTALTRQIPPEAWLLYFATCCWAIVYDTEYAMADRGD
                     DIKLNLKSTAILFGKADRIMIALFQLCMFLLLIGFGVDLGLGKIYYGSLFLASFFSVY
                     QQYLIKKRIPINCFKAFLNNNFLGGTVFLGLVLDYWLSH"
     gene            complement(5225..6217)
                     /locus_tag="PK389_00720"
     CDS             complement(5225..6217)
                     /locus_tag="PK389_00720"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HQY22267.1"
                     /translation="MKSIQSALFSIILFSLGTFWTGTATSTSRSLQNTVPSVVVSITP
                     FYALVAAVMEGVGAPKLLVKPGASPHEYALRPSDILLLQDAELIFWGGPQLETFLVKP
                     LNTLKAHSEAHHIVEFDNAPGLLLLPIRHSTAWDLHEHEHGNGHTHEVLDNDKSSQDM
                     HFWLNPNNAIILTDIIVHHLAMIDVKHTHIYKKNGTQLKQRLKKLDAHIATELATVKT
                     ISYIVFHDAYQYFEQRYGLKGVGSITLNPEIPPSAKRLNTIRTTIEKSKAQCVFREPQ
                     FQPKLVDSIVVDLGVKTGELDPIGQASQNNSEGYFTLLENLAHAIKQCLSSVQK"
BASE COUNT         1962 a         1060 c         1339 g         1975 t
ORIGIN      
        1 tgacgattta ttttttagca ttacctttct tcttacattt tgcaaaaaaa ttgaaacaaa
       61 aagaacatat ggatatctat gataaaggaa caaaatttaa tttctttaga ttagacacac
      121 attatgcgcc gaccgcgaat cagtttaaat aataaaagat ttgttacgtg cttctaaaaa
      181 cagatagata gcttgtcatt aggtgtttct aaaattattt accaaaaatc attggacagc
      241 tctcatcatt atgtaaaaag atttaaagac cggttattta tacagttata agtctaaaaa
      301 aatgtttaag attggtgttc taatctcaca gaaaaactaa aaaatgatct aagtttaaag
      361 tatgagccta aactatgtat tccgtcctat tcaagagagt gatatgcccg ctattttaga
      421 gatagcagaa cagaaagggg ctgggtttaa ttctttgcct aatgatgcta aagtgatgca
      481 agcaaagatt atccagtcca ttaatagttt ttctgaaaaa cttgatttta ataaacgata
      541 ttattttttt gtactggaga acagtgacac ccaagaaatg atgggttctg ctagcataga
      601 aacgaatctt ggatacctat ggccatttta taaatataaa atatcgacac ttgtacaaat
      661 ttcaaaagct ttaaatcaac ataaagaaca tcaaatattg agtttagtga ccgagcatca
      721 gggtgccacg gaattaggtg cattatattt aaaacctgct tatcgtggag aaggccgggg
      781 tacttttctg tctcgagcac gctgtttatt tattgctgag tttttaaata atttttctga
      841 aaaaatagtt gcagatatgc gtggcatgag taatcaagac gagatttctc cattttggga
      901 agcgataggt cattattttg cagatatacc ttattcagag gccagtcatc taaaagcgac
      961 aaagggctca caatttctcg tagatttgat gccgcattta cctatttata ttgatctttt
     1021 gcccgaaata gccaaaaaag ttattggtaa agtacataaa aataccgcgc ctgccttaca
     1081 tgtgctagaa aaagaaggct ttcaatttaa aaatcatgtt gatatttttg atgcgggagt
     1141 gactattgaa gctgaaaaat tgcatcttaa aacagtacga gaaagcaaaa aagcggctct
     1201 agttgctttt aaagatagtg taaaaaaaga acccacgatg atgattagta atacaaaaca
     1261 caatcatttt cgtgcagtcg ttggcgcatt agaagaacta cgagaaaagg aaatagcgtt
     1321 aagcacagca gtggcaaaac aattgcagat ttctgtaggt gataagttga ggtattgttc
     1381 tcttcgatag agggtgaatt gcaattatga atggtcgaaa ttttataggc ggtcgttgga
     1441 cagaaggaac aggtcctggc tttgaatcag aaaaccctgc aacgggtgaa gtgatttggc
     1501 gcggatatag tgcagataat aggcaaatta acaaaactgt acattttgca aagagcgcgt
     1561 ttcaaagttg gtcggcgctt tccttagaaa aaagaatgga ttatttggaa agattttgtg
     1621 aggaactgaa aaaaaatcgg ttattgcttt caacgacaat cgctttagaa acgggtaaac
     1681 cactttggga atcagatatg gaagtgggtg cgatgatcaa taaattaccg atatccgtta
     1741 aagcctatca ggagcggtgt cgagagcggg ttgaaaccct atcgagcggt gtttcaataa
     1801 cccggcatag accacatggt gttatagcgg tattgggtcc ttttaatttt cccggacatt
     1861 tgcctaatgg tcatattatt cctgcactat tagctggtaa taccgttgtt ttcaaatcca
     1921 gtgaattaac gcccaaggtt gcagaaaaaa tatttttttg ttgggaacag gcaaaattac
     1981 cggaaggtgt tattaacctg atccagggag gcagagaaac tggagagata ctctcgaagc
     2041 atccggattt gaatgggtta atgtttacag gaagttttaa aacgggtcat gcactagcca
     2101 atgtgttttc cgaaaaagca gggaagatat tagcactaga aatgggtggc aataatccat
     2161 tggtggttca cgaagtgagt gatattaaag cagcggcact gcaggttatt cagtccgcct
     2221 atattactgc agggcaacgg tgtacgtgtg caagacgttt gattgtaaca aaaggatgga
     2281 atggacaaga gtttattgaa gaattaataa aaatgacgaa cagcttaaaa attggcccgt
     2341 atacggatca gcctgagcca tttatgggtc cgctgattag caatatttct gcaaaccatg
     2401 tcatgacagc ctatgattta ttaattcatg aaggggcaaa atctttaatg cccttaaaga
     2461 gaattgacga aaatttaccc tttctttccc caggactatt agatgtcagt gctattaaag
     2521 tccgtacgga cgaagaaatt tttgggcctc tgttgcaatt gacttgggtg gacgattttg
     2581 aggcagcatt gatcgaagcc aataaaacag catatggact agctgcggga ttgttgtgtg
     2641 ataacgtaga atattatgaa tattttcggc ataaaataaa agcaggcatt atcaattgga
     2701 accatgcgct gacaggcgca agtagttcgg caccttttgg cgggcttggg cacagtggta
     2761 accatcgtcc cagtgcttat tatgctgctg attattgtgt gtatcctgtc gcaagcttag
     2821 aagaaaatat tttaaaaata ccagataatc tgccgcctgg aattcagtta tgaagaccag
     2881 tgaaattaat ttcgatggca ttattgggct aacacatcat tattcaggct tggcttttgg
     2941 caatttagct tcgaaagcaa atgcacataa aatttcaaat ccaaaagcgg cggcactcca
     3001 aggcttgaaa aaaatgcact tgctaatgca acggggtatt ttacaggcag ttttaccgcc
     3061 gcatgaacgg ccgctattat catttttaaa aaatttagga tttacaggaa cggatgctaa
     3121 aatactgaaa gctgtttatg cattagaccc acacttgttt ggcgctgcat gttctgcatc
     3181 cagtatgtgg gttgcgaatg ctgcaacagt gacgcccagt tcagatagcg cagattgtcg
     3241 agtacatatt acgcttgcta atttaatgag caacctgcat cgcgcattag aagctgaaac
     3301 aaaccttaaa atatttaaaa agattttttc agatgagaat tattttaaga tacatccacc
     3361 cttactttct caggttgact tttccgatga aggtgctgca aatcataatc ggctttgtca
     3421 ggactatggg tcgacaggat taaatatatt tatatatggt cgcagtggcc ttcagaaaaa
     3481 agaaatattg ccgaagcaat acccagcgag acagacgcgg gaagcttcaa aagcagttgc
     3541 gcgcttgcat cagctacgtc cagaaaatac gttttttata aaacaaaacc ctatggctat
     3601 cgatcagggt gtttttcata acgatgtgat ttcagttgca aatcaaaatg tttttttatt
     3661 tcatgaattg gcttttacag atatgcctga attagttgat ttattgaaaa aaaaatcaca
     3721 ggattctttg tatttattgc cggttgcgac ggccgattta agtctgcagg atgcggttaa
     3781 atcttacata tttaatagtc agttaattac ccttgatacg ggggtaatgg cgctgattgc
     3841 acctattgaa agccgtgaaa acaaaaacgc ccatcaagtg ctattaaaaa taatggcagg
     3901 aaaaaatcct atttcagagc tccattttgt tgattgtagg cagagtatgt taaatggcgg
     3961 tggtcctgcc tgtttgcgtc ttcgggttgt tttaacggaa gcagagcaga aggcttgttc
     4021 tgcgcgggta tttttagatg aagctttata tcaacgcttg gtgcagtgga tcgagcgaca
     4081 ttatcgtgat aggttgtctc taggggattt agtggacccc aggctgttaa atgagatcta
     4141 tgtcgcattg gacgagttaa cacagatttt aaaactcggc actctttaca attttcaaaa
     4201 aatatgaaga taataggcat ctagcttttc agataatgat tttagtgagc ggatgcatgt
     4261 ttaagcgcag caactttagt agcgaatcaa aagcattatc tgaaaagcgc gttgcattaa
     4321 taagtttcgg aaaatccatc gtgaaaaatc aaaaaataat attttttatt cgcttaatgc
     4381 gattagacaa acctattggt atttttttgt tattatggcc aatgttaatc gcattgtggg
     4441 ttgcaggaaa aggtaaaccc aaccctacaa tagtttgtat atttattttg ggtgcgatta
     4501 tcatgcgaag tgctggctgt gtaattaatg atttatttga tagacgtttt gatatctccg
     4561 tagaacgcac taaaaatcgc ccattagcag tgggcgcaat tagtataaaa gaagcgctgt
     4621 ttgttttttt tacattgatt tttttcggat ttttactggt tttacagttg aatattttaa
     4681 caattatgat gtctatcata gccctattat tagctattgt ttatccttta atgaaacggt
     4741 atacacattt agcacaagtg gtgttgggtg ctgcttttgg ttgggccgtg ccaatggcct
     4801 ttacggcatt aacacgccag atcccacccg aggcatggtt actctatttc gcgacctgtt
     4861 gttgggcaat tgtttatgat actgaatatg caatggccga caggggtgat gatattaagc
     4921 ttaacttgaa atccactgct attttatttg gcaaagcaga tcgtatcatg attgctctat
     4981 ttcagctctg catgttttta ttattaatag gctttggagt agacttaggt ttaggtaaaa
     5041 tatattacgg tagtttattt ttagctagtt ttttttcggt atatcagcaa tatttaatca
     5101 aaaaaagaat tcctatcaat tgctttaaag catttttaaa taataatttt ctgggtggaa
     5161 cggtattttt gggtctggtg ttagattatt ggctgagcca ttaagggcct gtacaaaaaa
     5221 caagttattt ttgtacagaa ctcaagcact gtttaattgc atgcgctaaa ttctctaata
     5281 atgtaaagta cccttcggaa ttgttttgag atgcctggcc aataggatcc aattctcctg
     5341 ttttaacacc caaatctacg acaatgctat ctactagttt aggttgaaat tgaggctctc
     5401 taaatacgca ttgcgctttt gatttttcaa tggtggttcg aatcgtattt aagcgttttg
     5461 cactaggcgg tatttcagga tttaatgtaa tagagccaac accttttaag ccatagcgtt
     5521 gttcaaaata ttgataggca tcgtgaaata caatatatga aatcgttttg acagttgcta
     5581 attcagtggc aatgtgcgca tctaattttt ttaaacgctg ctttaattgt gtaccatttt
     5641 ttttgtagat atgtgtgtgc ttgacatcaa tcattgcgag atgatgcaca ataatatctg
     5701 ttaaaatgat cgcattgtta gggttaagcc aaaaatgcat atcttgactt gatttatcat
     5761 tatctaagac ttcatgtgtg tgtccgtttc cgtgttcatg ttcgtggaga tcccaagcag
     5821 tgctgtggcg tattggtaaa agtaaaagcc ctggagcatt atcgaattcg acaatatgat
     5881 gtgcctctga atgcgcttta agcgtattta aaggttttac taaaaaagtt tcaagctgag
     5941 gcccccccca aaatatcagc tcagcatcct gaagcaatag aatatccgaa ggccttagcg
     6001 cgtattcgtg cggggaagca ccaggtttaa ctaataattt gggtgctcca acaccctcca
     6061 taacagcagc gactaaagca taaaatggcg ttatactaac gacaacgctt gggacagtgt
     6121 tttgtaaaga tctagaggta cttgtggcag tgcctgtcca aaaggtccct aggctgaaga
     6181 gaatgatcga aaataaagcg gattgtatcg atttcataaa tatgttaaat actcaactta
     6241 aaggatggtc tacctttgcc cttagtaact gctaatcaga tttctgttca gtttggacaa
     6301 aaaacgatct taacagatgt gcatcttacc gtacgt
//
LOCUS       DAORVT010000018         6171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_8236_length_6171_cov_3.490190, whole genome shotgun sequence.
ACCESSION   DAORVT010000018 DAORVT010000000
VERSION     DAORVT010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6171
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8236_length_6171_cov_3.490190"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /gene="tig"
                     /locus_tag="PK389_00725"
     CDS             <1..1281
                     /gene="tig"
                     /locus_tag="PK389_00725"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HQY22268.1"
                     /translation="VENTSTIGRRVTVTVPADRVRLEIVKQTKSVQQNGKIAGFRQGK
                     MPAKMIQDKFGAQIRNEAISKIIETTLPNALLENALEPAGRPEIEEVKNMAEVDKDIT
                     YVVRFEIYPEINLPDFKTIQVEKYEVEITEADVDKAILNLRNQMATWTVVTRPAQMGD
                     KLLVDYTSTLQGKPYENNAGKDVSVELGKNVFIEGFESGLLGAVTGETRECELHFPKE
                     WRIEKLAGQPVHFSIQVKEISEQHLVELDEAFANKIGATGSDLSAIRAKIRGNLEKQV
                     AHAVQETIKNALLSKILTLVDLPLPKALLETEIAALHEDLHRRNGNQAQESCHHHGLE
                     DEAKRRLALSLILQKIIKLENLTLDNTKVRQKISEIAGSFGNAEFIEKMYYESEELLA
                     GIQNSVLVEQAVNLILGQASLISKPITVEDLFKL"
     gene            1314..1913
                     /gene="clpP"
                     /locus_tag="PK389_00730"
     CDS             1314..1913
                     /gene="clpP"
                     /locus_tag="PK389_00730"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010490214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HQY22269.1"
                     /translation="MTDIKATGLVPIVIEQSARGERAYDIYSRLLKERVIFLVGPVED
                     YMANLVIAQMLFLESENPDKDIYLYINSPGGAVTAGMAIYDTMQFVKPDISTLCIGQA
                     CSMGALLLASGAKTKRRSLPNSRIMIHQPLGGFQGQATDIEIHTREILKVRHTLNKIL
                     AKHTGQSLGRLEKDTDRDNFMGPEEACEYGLIDTVITER"
     gene            2022..3305
                     /gene="clpX"
                     /locus_tag="PK389_00735"
     CDS             2022..3305
                     /gene="clpX"
                     /locus_tag="PK389_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HQY22270.1"
                     /translation="MGDKQNGRNDGKLLYCSFCGKSQNEVRKLIAGPSVFICDECVEL
                     CNDIIREEIEEKEGATVTTRLPTPKEINRILDQYVIGQTHAKKVLAVAVYNHYKRLQS
                     GLKLKEDDVELNKSNILLVGPTGSGKTLLAETLARLLDVPFTIADATTLTEAGYVGED
                     VENIIQKLLQKCEYNVEKAQTGIVYIDEIDKISRKSENPSITRDVSGEGVQQALLKLI
                     EGTIASVPPQGGRKHPQQEFLQVDTSGILFICGGAFSGLEKIIRQRSEKGGIGFSAEI
                     SSKDDQKSLSDSLKQVEPEDLIKYGLIPEFVGRLPIVATLEELDENALVQILTEPKNA
                     LTKQYHKLFEMEGIEIEFRESALKKVAQKAMERKTGARGLRSILEYVLLDTMYDLPSL
                     EDVSKVVIDESVVNGDSEPLLIFENTNKPVRQANE"
     gene            3314..5722
                     /gene="lon"
                     /locus_tag="PK389_00740"
     CDS             3314..5722
                     /gene="lon"
                     /locus_tag="PK389_00740"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HQY22271.1"
                     /translation="MSELTVLPVLPLRDVVVYPHMVIPLFVGRKKSIEALEAAVEKSR
                     HILLTAQKEAVDDSPTEKSLYVIGTIANILQLLKLPDGTIKVLVEGIERGRLIDFVHT
                     EPFFLANVESIPAVAVENQEIEVLIRSLLSQFDQYVKVSNKFSSEVIMSLTGIENPVR
                     LVDTIAAHLPLKIQDKQKILEILNFKDRILELIQMIESEVELMQMEKKISGTVKRQIE
                     KNQREYYLNEKRKAIEKELGDLGDESGGKNDIKELLDKIEAAGMSKEAKIEALKEVEK
                     LKMMSPLAAEANVSRNYIDWMLAVPWKYRTKIRGDLLRAEAILNKDHYGLEKVKERCL
                     EYLAVQLRVPKMKGPLLCLVGPPGVGKTSLGQSLARATGRKFVRMSLGGVRDEAEIRG
                     HRRTYIGALPGKILQKLAKVKVKNPLFLLDEIDKMAMDFRGDPASALLEVLDPEQNHA
                     FNDHYLEVDYDLSDVMFVATANSLNIPLPLLDRMEVIRLSGYIEDEKLHIAEQYLIPK
                     EMIANGLKPKELKITDEAIIDIIRYYTREAGVRSLEREISKICRKVVKDLLLSKSVKE
                     MTVDVDRLEHYLGVRRYRFGQAEAQDQIGQVMGLAWTEAGGELLTIEAMAVPGKGKVT
                     STGKLGKVMQESIQAAMTVVRSRGNVLGIADDFYEKHDIHVHVPEGATPKDGPSAGIT
                     MCTALVSVLTGIPVRKDVAMTGEITLRGQVLPIGGLKEKLLAARRAGILNVIIPDENK
                     PELSEIAKEVYEGMTIHAVHWIDEVLELALTRRPTPSDVKADIPSNSLPVKPITKDQV
                     PPVH"
     gene            5816..6127
                     /locus_tag="PK389_00745"
     CDS             5816..6127
                     /locus_tag="PK389_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012604504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQY22272.1"
                     /translation="MIKGRFMNKGQLVDALSSAEDLSKSKAASVVDAFLDVLTKTMAT
                     GEQVAIAGFGIFTVKKRPARVGRNPRTGGDLQIPEAMVVGFKAGKALKDAVNEKKKEA
                     A"
BASE COUNT         1971 a          999 c         1501 g         1700 t
ORIGIN      
        1 gttgaaaata cgagtactat aggacgtcgg gtaactgtta cggttccagc cgatcgtgtt
       61 cgtttagaaa tagttaaaca gactaaatca gtgcaacaaa atggaaaaat tgcgggtttt
      121 cggcagggaa aaatgcctgc aaaaatgatt caagataaat ttggtgcaca aattcgaaac
      181 gaagcaatca gtaaaattat cgaaacaacg ttgccgaatg cacttctcga aaatgcacta
      241 gagcctgcgg gtcgtccaga aatcgaagaa gtcaaaaata tggccgaagt ggataaagat
      301 attacgtatg tggtgcgttt tgaaatatat ccagaaatta atttgcctga ttttaaaacc
      361 attcaagtcg aaaaatatga ggttgaaatt accgaagccg atgttgataa ggcgattttg
      421 aatctgcgta atcaaatggc aacgtggacg gttgttacaa gacctgcaca gatgggtgat
      481 aaattattgg tggattatac cagtacatta cagggtaaac cgtatgaaaa taatgctggc
      541 aaagacgttt ctgtagagtt gggtaaaaac gtttttattg aaggctttga atccggattg
      601 ttaggcgcgg taacgggtga gacgcgagag tgcgagcttc attttccaaa agaatggcgt
      661 atcgaaaaat tggcaggtca acctgtacac tttagcattc aggtgaaaga gatcagtgaa
      721 cagcacttag ttgagctgga cgaagccttt gcgaataaaa ttggcgcgac tggatctgat
      781 ttatcggcca ttcgtgcgaa aattcgaggg aatctcgaaa aacaagttgc acacgctgtt
      841 caagagacca taaaaaatgc actcttatct aaaatattaa cgttagttga cttgccttta
      901 cctaaggcat tgttagaaac agaaattgcc gcattacatg aagatttgca tcgaagaaat
      961 ggcaaccaag cacaggagag ttgtcatcat catgggctag aggatgaggc taaacgacgc
     1021 cttgccttaa gtcttatttt gcaaaaaata attaagttag aaaatttaac actagataat
     1081 acgaaggttc gtcagaaaat ttctgagatt gcaggttctt ttggcaacgc agaattcata
     1141 gaaaagatgt attatgaatc tgaagagttg ttggctggaa ttcagaattc tgtgttagtt
     1201 gaacaagcag taaatttgat tttaggacaa gcatctttga tttcgaaacc tatcacggtt
     1261 gaggacctat ttaaattgta gagacagatc tatcatcaaa aaggggattg caaatgacag
     1321 atattaaggc aacaggattg gtaccgattg ttatagaaca gtccgctcgt ggcgagcgtg
     1381 cctatgatat ctactcacgg cttctaaaag aacgcgtgat ttttttagtg ggccctgtag
     1441 aagactatat ggcgaactta gtgattgccc agatgctatt tttagaatca gaaaatccag
     1501 ataaagatat ttacttatat attaactcgc cgggtggagc cgttaccgct ggtatggcga
     1561 tttatgacac catgcaattt gtgaaacccg atattagcac tctttgtata gggcaggcat
     1621 gcagtatggg agcactcctt ttggcaagtg gtgcaaaaac aaagcgacga tcactaccaa
     1681 attcacggat tatgatccat cagcccttag gtggctttca agggcaagcg actgatatcg
     1741 agatccacac tagggaaatt ttaaaagtcc gccatacgct gaataaaata ctggctaagc
     1801 atacaggcca gagtctgggg cgccttgaaa aagataccga tagggataat tttatgggac
     1861 ctgaagaagc atgtgaatac ggtcttattg atacggtgat aacagaaaga taatcctctg
     1921 aaacgttagc acttttttcg cattcaatct taatgagggc ttgaaaagtg cttttgccgc
     1981 ccttatgtta gacaataacg atcttttatg tgaggtatcc catgggtgac aagcaaaatg
     2041 gccgaaatga tgggaaatta ttatattgct cattctgtgg taaaagccaa aatgaagtta
     2101 gaaagcttat tgctggtccg tctgttttta tttgcgatga atgtgtggaa ttgtgtaatg
     2161 acattattcg tgaagaaata gaagaaaaag aaggagcgac ggttacgacg aggcttccaa
     2221 cgcccaaaga aattaatcgt attttggatc aatatgttat tgggcaaaca catgctaaaa
     2281 aggtacttgc cgttgcggtt tataatcact ataagcgttt acaatccggg ctaaaattaa
     2341 aagaagatga tgtggagttg aataaaagta atatcttgtt agttggacca acgggtagtg
     2401 gtaaaacgct gttagcagag actttagcgc gtcttttgga tgtgcctttt acgatagcgg
     2461 atgcgaccac cttaacagaa gccggatatg ttggtgaaga tgttgaaaat attattcaga
     2521 agttactgca aaaatgtgaa tacaatgttg aaaaagcgca aacgggaatt gtctatatcg
     2581 acgaaatcga taaaatttcg cgtaaatcag aaaatccatc gattacacgt gatgtttcgg
     2641 gtgaaggtgt tcaacaagct ttattaaagc tgatagaagg cactattgct tctgttccac
     2701 cgcaaggtgg acgaaagcac ccgcagcaag aatttttaca agttgacact tcgggtattt
     2761 tatttatttg tggtggcgca ttctcgggtc ttgagaaaat tattcgtcaa cgatcggaga
     2821 aaggggggat tgggttttcg gctgagattt cgagcaaaga cgatcaaaaa tctttaagcg
     2881 atagcttaaa gcaggtggag cctgaagatt taattaagta tggtcttatt ccagaatttg
     2941 taggtcggct accgattgtc gcaacgctag aagaattaga tgaaaatgcc ttagttcaaa
     3001 tattaacaga gcctaagaac gcgttaacaa agcaatatca taagctcttc gaaatggaag
     3061 gaatagaaat tgaatttaga gagagtgcac ttaaaaaagt ggctcaaaaa gcgatggaaa
     3121 gaaagaccgg tgcaagagga ttacgttcta ttttggaata tgtcctatta gatacgatgt
     3181 atgatctgcc ttctcttgaa gatgtctcga aggttgttat tgatgaatct gtggtgaatg
     3241 gtgattcaga gccattgtta atttttgaaa atacgaataa gccggttcga caagccaacg
     3301 aatagaggga tatatgagcg aattaacagt gctgccggta ttgccattaa gagatgtggt
     3361 tgtttaccca catatggtta ttcctttgtt tgtgggtaga aaaaaatcca tcgaagctct
     3421 tgaagctgcg gttgaaaaat cgcgacatat tttattaacc gctcaaaaag aagctgttga
     3481 tgattctcca accgagaaga gtttatacgt gattggaacc atcgcgaata tactgcaatt
     3541 attgaaatta cccgatggta ctataaaagt gttggtagag ggaattgagc gtgggcggct
     3601 gattgacttt gtgcacacag aacctttctt tttagctaat gttgaatcaa ttcctgctgt
     3661 tgctgtagaa aatcaagaaa tagaagtctt gattcgttct ttattgtctc agtttgatca
     3721 atatgtcaaa gtcagcaata agttttcatc agaagttatc atgtctctga cgggaataga
     3781 aaatcctgtg agactggtgg atacaatagc agcgcatctg ccccttaaaa tacaagataa
     3841 acaaaaaatt ctcgaaatat taaactttaa agatcgcatt cttgagctca tacaaatgat
     3901 agagtctgaa gtagagctga tgcagatgga aaaaaagatc agcggtactg taaagcgtca
     3961 gattgaaaaa aaccaacgcg aatattattt aaatgaaaaa aggaaagcga ttgaaaaaga
     4021 actcggtgat ttaggcgacg aaagtggtgg gaagaatgat attaaggaat tgctcgataa
     4081 aattgaagct gctgggatgt ctaaagaagc taaaatagag gctttgaaag aagttgaaaa
     4141 attaaaaatg atgtctccac ttgcagcaga agcgaatgtt tctcgcaatt acatcgattg
     4201 gatgctggca gttccttgga agtatcggac taaaattcga ggagatttgt tgcgtgcaga
     4261 agccatttta aataaagatc attatggtct agaaaaagtt aaagaacggt gtttagaata
     4321 tttggcagtg caactccgtg ttccaaaaat gaaggggcct ttgttatgtt tagtgggtcc
     4381 tccgggtgtt ggtaaaactt ctttaggtca atcgctggcg cgcgcaacgg ggcggaaatt
     4441 tgtcagaatg tctttaggtg gcgttaggga cgaggcagag atccgagggc atcggcgcac
     4501 ttatattggg gcactacccg gaaaaattct tcaaaaattg gcaaaagtga aagttaaaaa
     4561 tcctttgttc ttattggatg aaatagacaa aatggcgatg gattttcgag gtgatcccgc
     4621 ctctgcgtta ttagaagtgt tagatcctga acaaaatcac gcttttaatg atcactatct
     4681 agaagttgat tatgatttat ccgatgtaat gtttgtggca actgcaaatt cactgaatat
     4741 tccactaccg cttttagatc gtatggaagt gatacgattg tccggctata tcgaagatga
     4801 gaagcttcat attgcagaac agtatctgat cccgaaagag atgatagcca atggattgaa
     4861 acccaaggaa ttaaagataa cagatgaggc tattattgat attattcgtt attatacgcg
     4921 tgaagcaggt gttcgcagtt tagagcgaga aatctcaaag atttgtcgta aagttgtgaa
     4981 agatctctta ttatcaaaat ctgtgaaaga aatgacagtc gatgttgatc gattagaaca
     5041 ttacttaggg gttcgacgtt atcgttttgg acaagcagaa gcgcaagatc aaatcggaca
     5101 agtcatgggg cttgcatgga cagaagctgg tggagaatta ctcaccattg aggcgatggc
     5161 ggttcctgga aaagggaaag tgacgagtac agggaaactc gggaaagtca tgcaagagtc
     5221 aattcaggca gcgatgacag tggtacggag tcgtggaaat gtgttgggga tagcggatga
     5281 tttttacgaa aaacacgata ttcatgtgca cgttcctgag ggcgcgacgc caaaggatgg
     5341 acccagtgcg ggaattacga tgtgtactgc attggtttcg gttctcacgg gtatccctgt
     5401 tcgtaaagat gtggctatga cgggcgagat tacgcttcgc ggacaagttt tgcctattgg
     5461 cgggttaaaa gaaaaactgc tggccgccag acgtgccggt attctaaatg tgattattcc
     5521 ggacgaaaat aagccggaat tatcagaaat agcaaaagaa gtttatgaag gaatgacgat
     5581 tcacgccgtc cattggatag atgaagtgtt agagttggca ttgacgcgtc ggccaacacc
     5641 cagcgatgta aaggcagata ttccgtcaaa tagtttgcct gttaaaccga ttactaaaga
     5701 tcaagtgcct cctgtgcact aatgggcttt aaataaattt gacggttggg ttctattgtt
     5761 ggtataaaac ttccagatta gcgtgatgtg tagcctcacc gcattctatt ttttaatgat
     5821 taaggggaga tttatgaata aaggtcagtt ggttgatgct ctaagctctg ccgaagattt
     5881 atctaagagc aaagccgcat ctgttgtaga tgcattttta gatgtattga caaagaccat
     5941 ggctacaggc gaacaggttg cgatagcagg atttgggata tttactgtta agaagcgccc
     6001 tgcacgtgta ggacgtaacc ccagaacagg tggtgattta cagatacctg aagcaatggt
     6061 tgtaggtttt aaagcgggga aggctttaaa agacgcggtg aatgagaaga agaaagaagc
     6121 ggcttaagtt aagtgaatta gggtgcttag ctcagttggt agagcgtcgc c
//
LOCUS       DAORVT010000019         6024 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_8609_length_6024_cov_3.629921, whole genome shotgun sequence.
ACCESSION   DAORVT010000019 DAORVT010000000
VERSION     DAORVT010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8609_length_6024_cov_3.629921"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1402)
                     /locus_tag="PK389_00750"
     CDS             complement(<1..1402)
                     /locus_tag="PK389_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22273.1"
                     /translation="MVRRPGDGVGGGGADDDDLFVGGAGEPNPRRGSVDIEDKVEVDA
                     ATFKQLILAEAPLAIEAVNTAKVALDKAQGEFNAAQATPANADRLEEARRQRDAALLA
                     ADAAIRGISKTVEDVGFHDLEDKLDNEAVEAAHAKSVRLRDFVDAVRRNPNAPVPVPA
                     EPLVVDEPAAPLAAALANATAGYAAASNSNPSTQYVNATGGTSLTGQSRATSENKADR
                     SIAEILEDFKRAIEDHQKEYSVDLKYELSEPKNNSFTVSKDGIELVTVKKNSNKSISY
                     EFSENGLDASMADVILRASKDTVLVLHSSNYKHIDAILSAAADPHNQNKKIKFDPSTE
                     ALVAKLTPAELALYPAIAARLGKGNATKAAADAIKTLDVATKTAADTDAAKVAAIAEA
                     KDAELAVTQATNKVTAAQVRVTEADRVLGTIPVPPAPEPGAMAAPVTEQKTKADEEKA
                     AADVVQAAADVVQAAAV"
     gene            1697..3898
                     /locus_tag="PK389_00755"
     CDS             1697..3898
                     /locus_tag="PK389_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22274.1"
                     /translation="MATTTAYINTQLTTDRKAQADTAEPSLKTRELAPYLASAIRYHY
                     KLEFHATLQKGAAIDFSGLDNKGKNVNPPLIDAILYYNDWAFEELLKKGADINKATKG
                     DFVPLFHAVSHGNLRIVRALIAKGADPSDYRQGTTCLSLALECLRSGTLLEPQSRWRY
                     ENESPDTNNTALKQQISDFFLQENAYTPPKPNQAIPFVAPEQIHVYSAGNLDIIGFLL
                     LKGVPPTMAVSETDRSPLAIVQNPTKYSDIFRSDPKEQLEYIGTETENPNILLFTMTL
                     VNKRFTGTIPSIVVHYDIEPAKKLIEAAALAHSNPQLQSQLDAVHAKLHGATAQFAEE
                     IKGVKEAFNYDEKQEIERFKLFIKGHPNAEEAYNTILSRLKSIHKSAQAVAGGGVVVS
                     EGSLGLVAKVLDVSTELVDAIPLVGAAASKFFGLAASATREVDSRRQTNIYENIAAMV
                     PATGAGRIFESVARKLTMAYLSQFQRLQTKEEARSHMSRPTELVQNTLKGRFISPAER
                     VAAFAIIWMMDDVFNNMHQLERELQAPNGLERLLLNVVTQRKPEQGVETFWNTIVAKL
                     GMNQIPIRTQSGAISAETWHPADFWTMPGVVVQNSSAVQYFVGNKTNVAKFGSRSGSL
                     EDVTALGLTRTTETLLPQASSSTATPSANSRLVTPPSPTVMYAYLNTPQASPSSSTTS
                     SVSTTSGTSTTSSPAASTQAPKPEPKLDNPPAPKRNRSPFGRLRAACLKDT"
     gene            complement(4058..5227)
                     /locus_tag="PK389_00760"
     CDS             complement(4058..5227)
                     /locus_tag="PK389_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HQY22275.1"
                     /translation="MLIRKAFKFRLKTNETVEKKLSQFSGCCRFLWNKALALNLERLR
                     NRQSILRYGELDYWSKLWKRSEEYGFLANCHSQILQQKLKDLDKAFMDAFDKKQPNKR
                     IPRFKKKGLDGGFKYPQGFKICGNQVYLPKIGWLRFFESRRIEGKAKNITISRHAGEW
                     YVSIQTDYEVEQPTHSSSKIVGIDLGVIRFATLSDKTFYKPKNSFKKLKRKLKLSQRK
                     RDRKVKFSKNWKKQNQKINRIHAKIANVRKDYLRWISTRISKNHAMIVMEDLKVRNMS
                     ASAKGDIENPGKNVKAKSGLNKAILDQGWGEFKRQLEYKQIWRGGHLILVNPKHTSQK
                     CSNCDNVCSDNRLSQSEFKCVACGHETHADFNAALNVLRAGHVRLACGDIKQVAA"
     gene            5251..5667
                     /gene="tnpA"
                     /locus_tag="PK389_00765"
     CDS             5251..5667
                     /gene="tnpA"
                     /locus_tag="PK389_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HQY22276.1"
                     /translation="MNHATPIRTGRHCVYLLHAHLVFVAKYRRTIFSKNMLVAMQLIF
                     DKVCQGFESTLIEFEGECDHVHLLVNYPPKVSLSKLINSLKGVSSRRLRQQFSEIHRY
                     YWKRMLWSPSYFAGSCGGVPLSVVKQYIQNQKSSLE"
BASE COUNT         1675 a         1403 c         1158 g         1788 t
ORIGIN      
        1 cgactgccgc tgcttgtact acgtctgccg ctgcttgtac tacgtctgcc gctgcttttt
       61 cttcgtctgc cttcgttttt tgctctgtca ctggtgctgc cattgctcct ggctctggtg
      121 ctggtggtac tggtattgtt cctagcaccc ggtctgcctc cgttactcgt acttgtgccg
      181 ccgttacttt gtttgtcgcc tgtgtcaccg ctagctctgc gtcctttgcc tctgctatcg
      241 ctgctacttt tgccgcatct gtatctgcgg cggtttttgt tgccacgtcg agtgttttta
      301 tcgcgtctgc cgctgctttt gtcgcatttc cctttcctag cctcgctgca atggctgggt
      361 atagcgccaa ttcggctggc gttagctttg ccaccagtgc ttctgttgaa ggatcaaatt
      421 taattttctt gttctgatta tgcgggtctg ccgccgccga taagattgca tctatatgtt
      481 tataattaga actatgtaat acaagcaccg tatcttttga ggctcgtaag atcacatctg
      541 ccatgctcgc gtctaatcca ttctctgaaa actcatatga aatcgattta ttagaatttt
      601 ttttaacagt gacaagttct atgccatctt tgctaaccgt aaatgaattg tttttgggct
      661 ctgataattc atactttaaa tcgacgctat actctttttg atgatcctca attgctctct
      721 taaagtcctc caggatctct gcaatgctac gatcagcttt attttctgaa gtcgctctgg
      781 attgtcctgt aagagaggtg ccaccagtag catttacata ctgagtagag gggttgctgt
      841 tactcgctgc tgcgtatcct gcggtcgcgt ttgccaaagc agcagcaaga ggcgctgcag
      901 gctcatcaac aacaagaggc tcagcaggca ctggcactgg cgcattaggg ttacgacgca
      961 ctgcgtctac gaaatctctg agacgcaccg atttagcgtg agccgcctct accgcttcat
     1021 tgtcaagttt gtcttcaaga tcgtgaaagc caacatcctc aactgtcttg cttatccccc
     1081 taatagctgc gtccgcagcc agcaaagcag cgtctctctg ccgacgtgcc tcttccagtc
     1141 tatcagcatt ggctggtgtt gcctgtgcag cattaaactc accctgtgct ttatcaagag
     1201 ccacttttgc cgtattcacc gcctctatgg caagcggtgc ttcggctaga atgagctgtt
     1261 taaaagtagc agcatcaact tcaaccttat cctcaatatc aacgctaccc ctcctaggat
     1321 taggttctcc cgcacccccg acaaatagat catcatcatc cgctccgccc ccaccaacac
     1381 cgtcacctgg tcttcttacc ataacgcttc ccgcctatca gttagaaatt tagttacccg
     1441 ctctgtaaca cacacacaaa tttgtaagac tttacaaagc accttacaaa tggtgtcggc
     1501 tgtacaaact aaaaccttaa gtacaattta aaagacctac ctttaaccat ttcccctaac
     1561 tagcctggcc aaaaccaaaa atcttgattt gtacccgtac ggcagcgtta taatgaatgg
     1621 cattactaaa gcccatatta ttaagttttg taacttttag cttaaatgca ataatcaggt
     1681 ttaggaggag atacagatgg ctaccacaac agcgtatata aatacccaat tgacaacaga
     1741 taggaaagct caagccgata ccgcagagcc tagcttaaaa acaagggaat tggctccata
     1801 tctagcaagt gcgattcgct accattacaa attggaattt catgccacgc tccagaaagg
     1861 ggcggccatt gatttcagcg gattagacaa taaaggcaaa aatgtgaatc ctcctctcat
     1921 tgatgccatc ctttattata atgattgggc ctttgaagaa ttattaaaaa aaggagcaga
     1981 tatcaacaaa gcaactaaag gcgattttgt tcctttattt cacgctgttt cccacgggaa
     2041 tctccgcata gtccgagcat tgatagcaaa aggagccgac ccgtcggatt atcgccaagg
     2101 aacaacttgt ttgtccttag ctcttgaatg tctaaggagt ggcacgttgc tggaacccca
     2161 atctcgctgg cgatatgaaa atgaatcgcc cgatacgaat aataccgcat taaaacaaca
     2221 aatatcggat tttttccttc aagaaaatgc atacacacct cctaaaccga atcaggcaat
     2281 tccttttgtt gcacccgaac agattcatgt ctattccgca ggaaacttag atattattgg
     2341 ctttttgtta cttaaaggcg tgccgccaac gatggccgtt tctgaaactg atagatcacc
     2401 actggccatt gtccaaaatc caacaaaata ttctgatatt tttcgatctg atcctaaaga
     2461 acaattggaa tacattggca ctgaaacgga aaacccgaac atcctgcttt ttacaatgac
     2521 attagtcaat aagcgattta ccggaacaat accctcaatt gttgttcatt acgacataga
     2581 acccgctaaa aaattaattg aagcagcggc gcttgctcac tcgaatccgc agttgcaaag
     2641 ccaattagat gccgttcatg caaaactaca cggcgcaaca gcgcaatttg cggaagaaat
     2701 taaaggtgtc aaagaggcct ttaattatga tgaaaaacaa gaaatagagc gcttcaaatt
     2761 attcatcaaa ggccatccaa atgcagaaga agcctataac accattttgt ctcgtttaaa
     2821 atccatacac aaaagcgctc aggcggttgc aggcggtggt gtggttgtta gcgagggttc
     2881 actcgggttg gttgcaaaag tgctggatgt cagcacggag cttgtagacg cgataccctt
     2941 agttggcgct gctgcctcga aattctttgg attagccgct tcggctacga gagaagtcga
     3001 cagccgtaga caaaccaata tatatgaaaa catagcagca atggttcccg ccacaggggc
     3061 aggcaggata tttgagtcgg ttgcccgaaa attaacaatg gcgtatctgt cacagttcca
     3121 acggcttcag acaaaagaag aagcccgatc gcacatgagc cgcccaacgg aacttgtgca
     3181 aaataccctt aaaggtcgat ttatttcacc tgctgaacgt gtagccgcat ttgccattat
     3241 atggatgatg gatgacgtgt ttaataatat gcatcagctt gagagggagc tacaagcgcc
     3301 caatggatta gagaggcttt tgttaaatgt ggtgacacaa cgcaaacccg aacaaggcgt
     3361 agaaacattc tggaatacga tagtagcaaa gctcggcatg aatcagatcc caatacgaac
     3421 ccaatccggt gcaatttcag cagaaacttg gcacccagcg gatttctgga cgatgcctgg
     3481 cgttgtggta caaaatagca gtgctgttca atatttcgtg gggaataaga ccaatgtagc
     3541 aaagtttggc tcgcgatcag gaagtcttga agacgtaaca gcgcttggct taacgcgaac
     3601 cacagaaact ttattgccac aggcttcaag ctcaacagca acgccttcgg ctaatagtag
     3661 acttgttacg cccccaagcc caacagtcat gtatgcttac cttaacaccc ctcaagcttc
     3721 acctagctca agcacaactt ctagcgtaag cacaacctct ggcacaagca caacttctag
     3781 tccagctgct tcaacacaag cgcctaaacc ggaaccgaag ctagataatc cacccgcacc
     3841 taaacgcaat agatcacctt ttggaagact gcgagcagcg tgtttaaaag atacgtgagc
     3901 atttaacgca caacgataga ttacttataa tctatcgttg tttgtaaatg ttgagcgcct
     3961 tcgtcaccaa tatgattatc tgacagttga catcctctcc gacctaaagg ccgaagattc
     4021 ctaattccac ccttaggtgt tcaggaggtt cttgggctta agcagcaacc tgcttaatat
     4081 ctccacaagc taaccggaca tgccctgccc ttagcacatt aagtgcagca ttaaagtcgg
     4141 catgtgtttc atgtccacat gccacacatt taaattcact ttgcgaaaga cgattatcgg
     4201 aacaaacatt atcacaatta gaacattttt gtgaagtgtg ctttgggtta actaagatta
     4261 gatgaccgcc gcgccatatt tgtttgtatt caagctggcg tttaaactca ccccaacctt
     4321 gatccaatat cgccttattt aacccagact tcgctttaac gtttttaccc gggttttcaa
     4381 tatcgccttt agcagatgcg ctcatgtttc ttaccttcag atcttccatt actatcatgg
     4441 cgtggttttt gctgatacga gtagagatcc agcgaagata gtctttgcgt acatttgcta
     4501 ttttagcgtg aatccgatta attttttggt tctgtttttt ccaattttta gaaaatttaa
     4561 cttttctatc tctttttctt tgagacaact ttaattttct ttttaacttt ttaaaactgt
     4621 tttttggttt gtaaaatgtt ttatctgaca gagtagcaaa tctaatcaca cctaagtcta
     4681 tacctactat tttacttgag gagtgagtgg gttgttctac ttcataatct gtttgaatag
     4741 atacgtacca ttccccagca tgacgagaaa tagtgatatt ctttgccttg ccttctatcc
     4801 gcctgctttc aaaaaatctt aaccaaccta tttttggaag atagacctgg tttccacata
     4861 tcttaaagcc ctggggatat ttaaaaccac catccaaacc tttcttttta aatctgggaa
     4921 tacgtttatt gggttgcttt ttgtcaaaag catccataaa agctttatct aaatctttta
     4981 acttctgttg caatatttgt gagtgacaat tcgctaaaaa accatattct tcagaccttt
     5041 tccacaattt actccaataa tctaattcac cataacgtaa tatcgattgg cgattacgga
     5101 gcctttctaa atttaatgct aacgctttgt tccacaaaaa acgacagcac ccagaaaatt
     5161 gagacagctt tttttccact gtttcatttg tttttaagcg aaatttaaat gccttgcgta
     5221 tcaacatttt ttttatttta caattggtca atgaatcatg caacacctat tcgaactgga
     5281 agacattgcg tttatctttt acatgctcat ttggtcttcg tcgccaaata tcgacgaacc
     5341 atcttttcaa aaaacatgtt ggttgccatg caacttattt tcgacaaagt ttgtcagggt
     5401 tttgaatcaa cgcttataga atttgaaggc gaatgcgatc atgttcacct tttagttaat
     5461 taccctccta aagtttctct ttctaaactt attaacagtc ttaaaggcgt atcttcacga
     5521 agattaaggc aacaattttc ggaaattcat cgatactact ggaagaggat gctttggtct
     5581 ccaagttatt ttgcaggctc ttgtggagga gtgccactgt ctgttgtcaa acaatacatt
     5641 caaaatcaga aaagttcttt agaatgatta ggaatttaaa aaaattttgc gcctatatcc
     5701 cccacctaaa ggaaggggtc ttggcgcatc gcatgataaa gcgtgggaga aaattaaaaa
     5761 agctatcaaa gcagcaaacc catcggaaat tctggaaatg tccacagatg acaatacaat
     5821 atattttagg cattttaaag caaaaaatct gtttaattac atcgtctcca agcgcagaga
     5881 ataccgctaa ttccaacagt ggcctgttgt ttgtttttca gaggaatttt gtgtgataaa
     5941 tatatggtcg ttttaccatt taaagttact ttttcttcta ctatttttat tgtcccaaat
     6001 ttgggagaaa aaccccgcgc ttcc
//
LOCUS       DAORVT010000020         5906 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_8886_length_5906_cov_3.466929, whole genome shotgun sequence.
ACCESSION   DAORVT010000020 DAORVT010000000
VERSION     DAORVT010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8886_length_5906_cov_3.466929"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PK389_00770"
     CDS             complement(<1..380)
                     /locus_tag="PK389_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007149315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HQY22277.1"
                     /translation="MRAMILAAGRGKRMGTLTDHTPKPLLSIKSKPLIVYQIEALRDA
                     GVEAFVINLGYLGHKIEAALGNGARFGVRIQYSLEDPVLETGGGVVKALPLLGRDPFI
                     VVSSDIYTDFPYKQLPKDPEGLVH"
     gene            complement(381..1361)
                     /locus_tag="PK389_00775"
     CDS             complement(381..1361)
                     /locus_tag="PK389_00775"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HQY22278.1"
                     /translation="MQREQLLDQWLKCVLKTDHFKRVSLAGDASARRYFRVAIEGEHY
                     VVMDAPPPEIPRVFADIAVALEQHGLQVPRVIHADFEHGFLLLSDFGDRLYLSELNEV
                     SADQLYQNAFEALLKIQQCQKKLPLFDEAFLYRQLGIFEEWYLKEHKGFTPSDLKSAQ
                     AILNPIYEIFFEVCRNQPQVFVHRDYHSRNLMILPENSPGILDFQDAMTGPMTYDLVS
                     LLQDCYIAWPRERIQNWVLDFYEKAKTEKLLTGPETYSEFLRWFDLTGLQRHLKNLGI
                     FARLHYRDNKSGYLNDIPQVLKYISETCHRYAELQPLLNFFETLRETAEV"
     gene            1479..3968
                     /locus_tag="PK389_00780"
     CDS             1479..3968
                     /locus_tag="PK389_00780"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HQY22279.1"
                     /translation="MRHILNRFINLVISLVCLPSLVYANGIGNTTPFDDWVCSEQSQT
                     LCHGCYLQEPLPFPGQTDQYLKTLPITITSDQAEFRPDGNSTLTGHVHLTEGNRQLFA
                     NHATIHRDPRKTQAIDFIQADGHVKITEPNMRVDGTSAEVFVEKNTQVIQDATYRLYD
                     RHSRGTATSVTVYDKKNMVLKKATYTTCAPFQKTWFLQAENVDLNKKTGRGRAHHTRL
                     YFYNVPIFYLPYLDFPIDNRRQTGFLFPSYGTSNRSGVEIGAPFYWNIAPNYDATLTP
                     TLYSERGLELKGQFRYLLANSEGELDAAILPNDRKYRTFKTQSLANPFGLPNNDPRIT
                     ALKTNNNREFLRAKHITRFNEHLTTDLLYQTVGDDNYFMDFGNTLGSASTTQLLQQGD
                     LLFQAENWTIRTRLQQYQTLHPFSGPVITDVYKKLPQISLQNSYVDLPCGLEWTTQGE
                     FSHFDHKKDPMTNNAFTVGDRFQLRPGLSLPIGAPGWFVKPRIQADFLGYSLTRDPND
                     RTTLANSNRLLPIFDFDSGLIFEREFSLQKETYIQTLEPRAYYLYVPYRNQNALPIFD
                     TSYPGFDYNQLYRENRFTGLDRLGDANQVTLGLTSRFLSEVTGRERLSLTAGQIFYFK
                     NRQVTICNPKTNSMCLNQEYPNGHTRAQSSFIGLARLNLIDNWSLSGNTEWDPYQKQM
                     DKKAAGIQYQPNERSVVNLGYQFLRRNLVDLNPITGVAQRLDQTDASIAWPLTQNWRV
                     LGRWHYDLHNRRSNETLMGIEHQGCCTAVRLVVSRFLEPFDNTRPNNPRRYAKAVFIQ
                     FVFKGLGGVGNSKVNSTLQRIPGYWWRENEY"
     gene            4061..5059
                     /locus_tag="PK389_00785"
     CDS             4061..5059
                     /locus_tag="PK389_00785"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQY22280.1"
                     /translation="MIRSRSVSLLLACSLGLLSFSLAALPPQKKLPVLDRMIASVNNE
                     AITESELNRQTELLLVRLRQTETPLPSLPVLHKQLLEKMILEKCQLQVAAGEGITIDE
                     KALNQAVTEIASRDNLSIAEMRQFLEEQGISFTQFRETIKTELLLSKIQQKEVGQSIT
                     ISKAEVDHFLASPAGQDQSGTEYHLSHILIALPEEPSSTEVQKTEASADALAKELKHG
                     ADFAKIAIAKSAGQQALEGGDLGWRTTAAIPSAFAKIAPTLRVGEVYGPLRNDGGYHI
                     IKLLAKRSPTQKQQNSTQARNKAMDTLYQRKFDELVVTWLRHLRSEAEIKIYLNET"
     gene            5049..5858
                     /gene="rsmA"
                     /locus_tag="PK389_00790"
     CDS             5049..5858
                     /gene="rsmA"
                     /locus_tag="PK389_00790"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HQY22281.1"
                     /translation="MKPDFSHRPRKRFGQNFLQNPHVIQQIISTVNAQPNDNLIEIGP
                     GLGVLSKFLLAQVNHLNVIEIDRDLASQLAISLGHSQKLTIHTQDVLHFDFNVFETKN
                     LRVVGNLPYNITTPLIFHLLKFESKIQDMHFMLQKEVVDRLCAEPNSKDYGRLSIMAQ
                     YHCDPEYLFSVDPSAFYPSPKVNSAFVRLIPRPFPIKATNITLLQNITRDAFNLRRKT
                     LSNALKPYLALKDFIALDLRPTLRPEVLSVTDFVKISNYIDQRLNAVQEKS"
BASE COUNT         1808 a         1350 c         1100 g         1648 t
ORIGIN      
        1 aaatgcacta agccttcggg atctttgggc aattgtttgt aaggaaaatc cgtataaata
       61 tcgctactca caacaataaa gggatctctt cctaacaatg gcaaagcctt tacaacaccg
      121 cctcccgttt ctagaacggg atcttcaagc gaatactgga tccgaacacc aaatcgcgca
      181 ccattaccca atgccgcttc tattttatga cccaaataac ccagattaat gacaaacgct
      241 tctacaccgg catccctcag cgcttctatt tgataaacaa ttaagggctt gctttttatt
      301 gacaacaagg gcttgggggt atgatcggtt aatgtgccca ttcttttgcc acggcctgcg
      361 gctaatatca ttgcacgcat ctaaacctct gctgtttcgc gaagggtttc aaaaaaattc
      421 aacaatggtt gtaactcggc atatcgatga caagtctctg atatatattt taatacttgt
      481 ggtatatcgt taagatatcc tgacttatta tcgcgataat gcaatcgcgc aaaaatgcct
      541 aaattcttta aatgccgttg taagcccgtt agatcaaacc accgcaaaaa ttcagaataa
      601 gtttctggtc ccgtcagcag cttttctgtt ttcgcttttt cataaaaatc aagcacccag
      661 ttttgaatac gttcgcgcgg ccaagcaata taacaatcct gtaatagcga aaccaaatcg
      721 tatgtcatag gacctgtcat ggcatcttga aaatctaaaa tacccggact gttctctggc
      781 aaaatcatta aattgcgcga gtgataatct cggtgcacaa aaacctgtgg ttgattccga
      841 catacctcaa agaatatttc atatatcgga tttaaaatag cttgggcgct cttcaaatcc
      901 gaaggcgtaa atcctttatg ttctttgaga taccattctt caaaaattcc caattgtctg
      961 tacaaaaaag cctcgtcaaa aagcggtaat tttttttggc actgttggat cttaagcaaa
     1021 gcttcaaaag cattttgata taattgatcg gctgagactt cattcagttc gcttaaatag
     1081 agcctatccc cgaagtccga taacaataaa aacccgtgtt caaaatctgc atgaattacc
     1141 ctaggcactt gtaaaccgtg ttgttctagc gcaacggcaa tatccgcaaa tacccggggg
     1201 atttccggag ggggtgcatc cataaccacg tagtgttcgc cctcaatagc cacacgaaaa
     1261 tagcgtctgg cactggcatc acccgctaaa gacacccgtt tgaaatggtc tgtctttaaa
     1321 acacacttta gccattgatc taaaagctgc tctctctgca tgttagctct ctctctttaa
     1381 taaagatttg cagtctacct agaaaatatg cgatggttta cacatttctt tgagcgaacg
     1441 agggcggaca cggtgatcca cccctacaac attaacctat gaggcatata ctgaaccgtt
     1501 ttattaacct tgtcatcagt ctagtctgtt tgccaagtct tgtttacgca aatggcatcg
     1561 ggaatacgac tccctttgat gattgggtat gcagtgaaca atcacaaaca ctatgtcatg
     1621 gatgttactt acaagaaccg ctccccttcc cagggcaaac tgatcaatat cttaaaacac
     1681 tgcccatcac gattacctct gatcaggctg agtttagacc cgatgggaat tcaacattga
     1741 cagggcatgt gcacttaacc gagggaaacc gtcaactctt tgcaaatcat gcaactattc
     1801 accgcgaccc acgcaaaaca caggccatcg attttataca agcagacgga cacgttaaaa
     1861 taacagaacc gaatatgcga gttgatggta cttccgccga agtgtttgtt gaaaaaaaca
     1921 cgcaagttat tcaagatgca acgtatcgtt tatatgatcg acacagcaga ggtactgcta
     1981 cctctgtcac cgtatacgat aaaaagaaca tggttttaaa aaaagccacc tatacaacct
     2041 gcgctccttt tcaaaaaaca tggtttttgc aggcagaaaa tgttgacctc aataaaaaaa
     2101 cagggcgtgg tcgcgcacac catacacgtc tttattttta taatgtgcct attttttatt
     2161 tgccttacct ggatttccct attgataatc gacgtcaaac cggttttctt tttcccagct
     2221 atgggacttc aaaccgatcc ggtgtagaaa ttggggcccc cttttattgg aatattgccc
     2281 caaattacga tgcaacctta acgcctacgc tttatagcga acggggttta gaacttaaag
     2341 gacaatttcg ctatcttctt gcgaatagtg agggcgaact cgatgcagcc attttgccta
     2401 acgatcgcaa atatcgaaca tttaaaacgc aaagcctagc aaatcctttc ggtctcccga
     2461 ataatgatcc gcgcattacg gcactgaaaa cgaataataa ccgtgaattt ctccgtgcaa
     2521 aacatatcac gcgttttaat gaacatttaa caactgacct actttatcaa acggtaggag
     2581 atgataacta ttttatggat tttggaaata cccttggatc tgccagtaca acacaattac
     2641 tacaacaagg ggatctgtta tttcaagctg aaaactggac aatacgaacc cgcttacaac
     2701 aataccaaac cctacatccc tttagtggac ctgtcatcac ggatgtttat aaaaagttac
     2761 cccaaatcag tttgcaaaat tcctatgtag atcttccctg tggtctagaa tggacaaccc
     2821 aaggcgaatt ttctcacttt gatcataaaa aagatccgat gacaaataat gcctttacag
     2881 taggcgatcg ctttcaatta cgccctggcc tttctttgcc cataggcgct ccagggtggt
     2941 ttgtaaaacc acgcattcaa gcagattttt taggctattc acttacacga gatccaaacg
     3001 atcgcacaac cctggccaac agcaatcgat tactaccaat attcgacttt gattctggat
     3061 taatttttga acgtgaattt agtctacaaa aagagactta catacaaaca ctagaaccac
     3121 gagcttatta tttatacgtt ccttatcgaa atcaaaatgc tttacccatt tttgatacca
     3181 gttatccggg ttttgattac aaccaactct atcgagaaaa tcgttttacg ggtctagacc
     3241 gcttgggaga cgcaaatcaa gtcacactgg gtctcaccag tcgatttctc tctgaagtta
     3301 ccggtcgtga gcgtttaagt ttaacagcgg gccaaatttt ttatttcaaa aatcgtcaag
     3361 tgaccatttg taacccaaaa acaaattcaa tgtgtttaaa ccaggaatat ccgaatggac
     3421 acacacgtgc acaatcttcc ttcattggcc tggctcgcct taatctcata gataactggt
     3481 ctttaagtgg caatacagaa tgggatccgt atcaaaaaca aatggataaa aaagcggctg
     3541 gtattcaata tcaacccaat gagcgttcgg tcgtcaatct cggatatcaa tttctgcgac
     3601 gaaaccttgt tgatctcaat ccgataaccg gggtagcaca acgtttagat caaacagacg
     3661 cctctattgc ttggccttta acgcaaaatt ggcgggtttt aggtcgctgg cattacgatt
     3721 tgcataaccg ccgttcaaac gaaaccctta tgggcataga acatcaaggc tgctgcactg
     3781 ccgtgcgcct cgttgttagc cgttttctag agccttttga taatacacgt cccaacaacc
     3841 cgagacgata tgcaaaggcg gtatttattc aatttgtctt taagggttta ggtggcgtag
     3901 gcaatagcaa ggtcaactct accctacaac gcattcccgg gtattggtgg cgtgagaacg
     3961 aatattaggg tagaatggcg gcatcttatt ttttaactga gagcgttgct tattcaggtt
     4021 atgttctacc acgaaaaagt tagttactgg actttatatt atgattagat ctcgcagtgt
     4081 ctcccttctt ttagcttgca gcctcggatt gttgtctttt tcgttagcgg ctctgcctcc
     4141 ccagaaaaaa ctgcctgtcc tcgatcgcat gattgcttct gttaacaacg aggcgataac
     4201 ggaatcggaa ttaaatcgac aaaccgagct tctattggtt cgactgcgac agacagaaac
     4261 acccttgcct tcgctgcccg tgctacataa acaattatta gaaaaaatga tcttagaaaa
     4321 atgccaactg caagttgcag caggggaagg catcacaatc gatgaaaaag cgcttaacca
     4381 agcggtgacg gagatcgcca gtcgagataa cctttcgatt gcagaaatgc gacaattctt
     4441 agaagaacaa ggcatttcat ttacacaatt tcgtgaaacc attaaaacag agcttttgct
     4501 ttcaaaaatt caacaaaaag aggtcggaca aagtattaca atctcaaaag cagaagttga
     4561 tcatttctta gcttcacccg caggacaaga tcagtccggt acagaatatc atttaagcca
     4621 cattttaatt gccctacctg aagaaccttc gtcaacagaa gtccaaaaaa cggaagcgag
     4681 cgccgatgcg cttgcgaaag aattaaagca tggcgcagat tttgcaaaaa tagccattgc
     4741 aaaatccgca gggcaacaag cgcttgaagg tggagactta ggctggcgca ctacggccgc
     4801 catacccagc gcctttgcaa aaatcgcgcc aacattacgc gtgggtgaag tctatggccc
     4861 tcttcgcaac gacggcggat atcatattat taaactttta gctaaacgat cgcccacaca
     4921 aaaacaacaa aattcgacac aagcgcgaaa taaggcaatg gacacactct atcaacgcaa
     4981 atttgatgaa ctcgtagtga cttggttaag acatttacga tctgaggcag aaatcaaaat
     5041 ttacttgaat gaaacctgat ttttctcatc gaccacgcaa acgttttggg caaaactttt
     5101 tgcaaaatcc gcacgttatt cagcaaatta tcagtactgt taacgcgcaa cctaacgata
     5161 atcttattga aattggacct ggtttaggtg ttttaagcaa atttttattg gcacaagtga
     5221 atcacctcaa cgttatcgaa atcgatagag atctggcaag tcaactcgcc atttctttag
     5281 gtcattctca aaaattgacg attcacacac aagatgtttt gcattttgat tttaatgttt
     5341 ttgaaactaa aaacctgcgg gtagtcggta atctccccta taatattaca acacccttaa
     5401 tttttcactt gctaaaattt gaatcaaaaa ttcaagatat gcactttatg ctacaaaagg
     5461 aagttgttga tagactctgc gcagaaccca atagcaaaga ctatggacga ctcagcatta
     5521 tggcgcaata tcattgtgat cccgaatatt tgttttcagt tgatccgagt gcattttacc
     5581 catcaccgaa agtaaattct gcctttgttc gattaatccc tcgccctttt ccgataaagg
     5641 ctaccaatat caccttgtta caaaatatta cacgcgatgc ctttaatcta cgtcgaaaaa
     5701 cattgtcaaa cgcactaaaa ccttatctgg cattaaaaga ctttatagca ctcgatctca
     5761 ggccaaccct gcgtccagaa gttttatccg tgacagattt tgtcaaaatc agtaattata
     5821 ttgatcaacg attaaatgct gttcaggaaa aatcctaaat gagcacctat gctatcggcg
     5881 atctacaagg ctgttttgat cctttt
//
LOCUS       DAORVT010000021         5856 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_8987_length_5856_cov_3.240303, whole genome shotgun sequence.
ACCESSION   DAORVT010000021 DAORVT010000000
VERSION     DAORVT010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5856
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8987_length_5856_cov_3.240303"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /gene="ybgF"
                     /locus_tag="PK389_00795"
     CDS             <1..401
                     /gene="ybgF"
                     /locus_tag="PK389_00795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HQY22282.1"
                     /translation="TEKSAYEAAYNLVRTKRYTEATSAFQNYLERFKTGEHAANAHYW
                     VGEIYMLEWQKNPTNKTLLDKAADAFSNITSQFPGNPKVPDALLKLGIIENEKGDPMA
                     ARKYFTDIKSRYPGSAAARIAETRLKALSP"
     gene            475..1632
                     /locus_tag="PK389_00800"
     CDS             475..1632
                     /locus_tag="PK389_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006870715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY22283.1"
                     /translation="MEDFLFLQDDFTPEERLIRDEVRRFVDRDVIPIMADAYETAQFP
                     QQFIPQCAALGLLSLKLPIEYGGSGRSAIAYGLVCQELERGDSALRSFISVQNALCMF
                     PIFTFGSEAQKQKFLPKMAAGKLIGCFGLTEPDAGSDPSSLRTQAKKVSGGWLLNGVK
                     RWVTNGTIADCAIIWAHTVEGIRGFIVEKDFPGCTRQEIKKKMSLRASISAELFFKDC
                     FVPESHYLPGTEIGLVAALHCLTEARYGIAWGAIGSAMACYEIALSYCKKRMQFNRPL
                     ASFQLVQKDLVTMFLEIQKALCLNLQLGRLKERGQCTYPMVSLAKLNACREALKIARM
                     ARNLLGANGITSEYAVIRHMINLESVFTYEGTDNIHHLIVGKYITGLDAFC"
     gene            complement(1645..2865)
                     /locus_tag="PK389_00805"
     CDS             complement(1645..2865)
                     /locus_tag="PK389_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HQY22284.1"
                     /translation="MKIAKRAKKVVKLILFFGLITLLSPFILAKLNFYGPSHATTPAS
                     ANTTISSVTLNAPVSYADAVALAAPAVVSIKTTQEVTVDQNPLMQDPMFRYFFGEQNG
                     GQGNGEWNGNEKNKPGNEQPKETLQGLGSGVIISEKGYVLTNNHVIREADTVIVTLAD
                     GRSADGKVVGTDPDSDLAVLKINLDKLPVITLGSSQKLRAGDVVLAIGNPYGLDKTVT
                     LGIISATERAGTDVGILENLLQTDAAINPGNSGGALINSFGQIIGINTVIYSRSGGNQ
                     GIGFAIPIDQATEVMNKLIEGKPIIRGYLGIMMQPINKEIRGYIDYKEGDGVYVRAVV
                     RGSPAQKAGLLPGDVIIKINNTVITDERMALRLVSGLIPGKNYPLEIFRKGEYLSFIV
                     TPSERKPAQPKRKE"
     gene            complement(2956..3204)
                     /locus_tag="PK389_00810"
     CDS             complement(2956..3204)
                     /locus_tag="PK389_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007161581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQY22285.1"
                     /translation="MNKSKPSKPFLREHITSALQDYFSKLEDEPARNIYSLVLNEVES
                     ALLAIVMKEVKGNQSRAAEWLGLNRGTLRKLLVKYDLS"
     gene            complement(3167..4123)
                     /gene="dusB"
                     /locus_tag="PK389_00815"
     CDS             complement(3167..4123)
                     /gene="dusB"
                     /locus_tag="PK389_00815"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HQY22286.1"
                     /translation="MQIGKHKLENNLILAPMAGVTDRPFRQLCKALGVGMAVSEMVSS
                     NSLLRGSQKTLRRANHDGETEPRSIQIAGADPFMMGEAARFNEAEGADIIDINMGCPA
                     KKVCNTLAGSSLLRDEPLVTRILEATVNAVSIPVTLKIRTGWDPSHKNGITIAKIAEQ
                     SGIQALAVHGRTRSCFFKGAAEYETIAAIKDAVKIPVIANGDIETPEKAKAVLEQTKA
                     DGIMIGRAAQGRPWIFKEITYYLKTGELLPEPTPAEIKAILLGHLQNLYQFYGEYTGS
                     MVARKHVSWYSRGQTGSARFRDYFNRLEKPHEQIEAIEAFFT"
     gene            4106..>5856
                     /locus_tag="PK389_00820"
     CDS             4106..>5856
                     /locus_tag="PK389_00820"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQY22287.1"
                     /translation="MFTDLHVCVIKTEWCTITKVRFLFKREHMLPQVPIGVSDFREII
                     ETRDSTGEPYLFVDKSLLIKDVIEDLTKVKLITRPRRFGKTLNMSMLHHFFAKTVHNK
                     PTEHLFKGLKIAEYAECMKHQGQYPIIFLSLKDIKSASFKSAYEDFCKLLSQAYEQHE
                     HLLSDSKLTQRQKNQYNGILNRDAPESDIRSALKDLTFYLYQGYGVKPIVLIDEYDTP
                     IQTAYVKNYYEEMVTLMREFLGAGLKDNSHLDRAILTGVLRVSKESIFSGLNNIKTYS
                     VLNDRYGEYFGFVEDEVSRLLIKTALDSHINDVKAWYNGYQIGDAIIYNPWSIVNYIQ
                     ERGKLSSYWVNTSDNALIKDLWVHSSELFKGQFELLLKDEQIIMLMDEHLALNNLDKS
                     EIAIWTLLAMSGYLKANFIKNGNNESLYYLKIPNKEVKDLYKRLIAEWLSGVNNAMVF
                     NTFLNNLLNGEMKDFEDGLRRILLQTFSAHDIKGKNSEKFYHGFMLGLLSGIDQNHYK
                     IDSNKESGLGRYDIILIPNDTSKLGIILEIKSIDESSSKNLKEAANEAIKQIDEKQYH
                     ITLLQHSIGIIVIPASS"
BASE COUNT         1753 a         1070 c         1261 g         1772 t
ORIGIN      
        1 acactgaaaa atcggcctat gaagcggcat acaacttggt tagaacaaaa cggtataccg
       61 aagcgacatc tgcttttcaa aattatttag aacgatttaa aacgggtgaa catgctgcca
      121 atgcgcacta ttgggtgggt gaaatctaca tgcttgaatg gcaaaaaaat cccacaaata
      181 aaacgttgtt agacaaagca gcagatgctt tttctaatat tacatcgcaa tttccgggta
      241 atcccaaagt accggatgct ttattgaaat tgggtattat tgaaaatgag aaaggcgatc
      301 caatggccgc tcgaaagtat tttacagata ttaaaagtcg ttatccggga tcagcggctg
      361 ctcgtattgc cgagacgcgt ttaaaggcgt tatcgcctta aggccctgag aaaaataact
      421 tgttttttct tagagcttaa gcctcgttat tcgtagcgct tttttggaaa aattatggaa
      481 gattttttgt ttctccagga tgattttaca ccagaagaac gcttaataag agatgaagtg
      541 cgtcggtttg tggatcgaga cgttatccct attatggcgg atgcttatga aactgcacaa
      601 tttccccaac aatttattcc acagtgtgca gcattagggt tgctcagcct taaactgccg
      661 attgagtatg gtggttctgg cagaagtgcg attgcttatg gtttggtgtg tcaagaatta
      721 gaacggggcg atagtgcctt gcgtagtttt atttctgttc aaaatgcctt atgtatgttc
      781 cccattttca catttggatc agaggcacaa aaacaaaaat ttttacctaa aatggcagca
      841 ggaaagttaa taggttgttt tggtttaaca gagcctgatg cgggatctga tccgagcagt
      901 cttcgcacac aagccaaaaa agtttcgggt ggttggttgt taaacggcgt taaacgctgg
      961 gttaccaatg gtacgattgc agattgtgcg attatatggg cacacaccgt ggaagggatt
     1021 cgcggtttta ttgtcgaaaa agattttcca ggttgtacga gacaagaaat taaaaaaaag
     1081 atgtcattac gcgcgtctat ttcagcagag ttatttttta aagattgctt tgtacctgaa
     1141 agccattatc tacccggcac tgagattgga ttagttgcag cactacactg tttaacagag
     1201 gcacgatatg gtattgcctg gggagcgata ggttctgcca tggcctgcta tgaaatcgct
     1261 ttaagttatt gcaaaaaacg tatgcaattt aatagaccac ttgcatcgtt tcaattggtt
     1321 caaaaggatt tggtgacgat gtttttagag atccaaaaag cactttgttt gaatttacaa
     1381 ttgggtcgtt taaaagaaag agggcaatgt acttatccca tggtatcgtt ggcgaagctt
     1441 aacgcttgtc gtgaagctct caaaattgcg cgaatggcca gaaatttatt aggcgccaat
     1501 ggtattacct cagaatatgc ggttattcgg catatgatta acctggaatc tgtctttacc
     1561 tatgaaggga cagacaatat tcatcacctc attgtgggca aatatattac gggattggat
     1621 gctttttgct aaggggcagc gcctctactc tttacgtttt ggttgtgcgg gttttcgctc
     1681 gctcggtgta acgataaagc tcaaatactc acccttgcga aatatttcca gcgggtaatt
     1741 ttttccgggt attaatcccg agactaaacg taaggccatg cgctcatctg taatgacagt
     1801 gttattaatt ttaataatca catcgccagg taataaaccc gctttttgtg cggggctgcc
     1861 acgtacaacg gcacgtacat aaacaccatc gccttctttg taatcgatat agccgcggat
     1921 ttctttattt ataggctgca tcataatgcc caaataaccg cgaataatcg gttttccttc
     1981 gatgagttta ttcatcactt cggttgcttg atcaatgggg atcgcaaaac caataccttg
     2041 atttccgccg gaacggctat agataaccgt attaatacca ataatttgac caaatgaatt
     2101 aatcaatgcg ccgccagagt tgcctggatt aatagcggcg tctgtttgta gtaaattttc
     2161 taaaatacct acatctgtgc cggcgcgttc ggtagcgctg atgatgccta gcgtgacggt
     2221 tttatcaaga ccatagggat ttccgatcgc aagtacaaca tcaccggctc ttaatttttg
     2281 ggaagaaccc agcgtgataa cggggagttt gtccaggtta atttttaata cagccagatc
     2341 gctatcggga tcagtaccga ctactttacc atccgcagaa cggccatctg ctaaagtcac
     2401 aatcaccgta tcggcttctc ggatcacatg attgttggta agcacatagc ctttttcgct
     2461 aataataacg ccagacccta atccttgtag cgtttctttg ggttgctcat ttcccggttt
     2521 gtttttttcg ttaccattcc attcgccatt accttgacca ccattttgtt cgccaaaaaa
     2581 atatcggaac atgggatctt gcatcagcgg gttttgatca actgtgactt cttgtgtggt
     2641 tttaatgctt acaaccgcgg gtgcggcgag tgctaccgca tcggcatacg aaaccggtgc
     2701 gtttagggta acagaagaaa tcgttgtatt tgcagaagcg ggcgttgtcg catgagacgg
     2761 gccataaaaa ttaagctttg caagaataaa gggtgaaagg agagtaatta aaccaaagaa
     2821 aagaataagt ttaacaactt tttttgcgcg tttcgctatt ttcattttca ggctctctct
     2881 ttgaattcag tggcaaggac ttattgtgta actgcaaaag accattatat attaaacaaa
     2941 agggacgtcg gaagtctaag acaaatcgta tttgacgagc aactttctta atgtgccgcg
     3001 attaagaccc agccattcgg cggctcgcga ttggttgccc ttaacttctt tcatcacaat
     3061 ggcaagcaaa gcggattcta cttcatttaa tacaagactg tatatatttc tagccggttc
     3121 atcttccagt ttagaaaagt agtcttgtaa ggcggatgtg atatgttcac gtaaaaaagg
     3181 cttcgatggc ttcgatttgt tcatggggtt tttccaagcg attaaaatag tcacgaaaac
     3241 gtgcgctacc agtttgtccg cgactgtacc aagagacgtg cttgcgagca accatagaac
     3301 cggtgtattc accataaaat tggtataaat tttgaaggtg acctaataag atggctttaa
     3361 tttctgcggg tgtgggttct ggcagcaatt cgcctgtctt aagataatat gtaatttctt
     3421 tgaaaatcca agggcgtcct tgtgcagctc ggccaatcat aatgccatcg gctttggttt
     3481 gttctaaaac cgcttttgct ttttcgggcg tttcgatatc accattggca atcactggga
     3541 tcttaacggc gtctttaatc gccgcaatgg tttcgtattc tgcagcgcct ttaaaaaaac
     3601 aggagcgtgt acgaccatgc accgctaatg cttgaatgcc gctttgttct gcaatttttg
     3661 caatcgtaat accattttta tggctgggat cccagccggt tcgaattttg agcgtaaccg
     3721 gaatagaaac ggcattaaca gttgcttcta agatgcgagt tactaaaggt tcatctctga
     3781 gcaacgaaga accggctaaa gtattgcaca ctttttttgc agggcatccc atattaatat
     3841 caataatatc ggcaccttct gcttcattaa atcgtgcagc ttcgcccatc atgaagggat
     3901 cagcacctgc gatttgtata gagcgaggct ctgtttcgcc atcgtgattg gcgcgtctta
     3961 aggttttctg gctgcctctt aacaaggaat tcgaagagac catttcagaa acggccatgc
     4021 caacgccaag cgctttacaa agttggcgga atgggcgatc ggtaacacct gccatggggg
     4081 caagtataag attgttttct aacttgtgtt taccgatttg catgtttgtg tcattaaaac
     4141 tgaatggtgt actataacaa aagtaaggtt tttattcaaa agagagcata tgctgccaca
     4201 agtgccgatc ggcgtcagcg attttagaga aatcatcgaa acaagagact ccacaggcga
     4261 gccgtatttg tttgttgata aatccttact aattaaggat gtgatagagg acttgacaaa
     4321 agtaaagctt attacgcgcc cgagacgatt tggaaaaaca ttgaatatgt caatgttaca
     4381 tcattttttt gcgaagactg tgcataataa gccgacagaa catttattta aaggcctaaa
     4441 gattgctgaa tatgctgagt gcatgaaaca tcaaggtcaa taccctatca tatttttaag
     4501 ccttaaagat attaaaagtg cctcttttaa atctgcatat gaggattttt gtaaattatt
     4561 aagtcaagca tacgagcaac atgaacactt attatctgac tcaaagctaa cccaacgtca
     4621 aaaaaaccag tataacggca tattaaatag ggatgcgcca gagtctgata tcagaagtgc
     4681 cttaaaagac cttacttttt atttatatca aggttatggt gttaaaccca tcgtgctgat
     4741 tgatgaatac gatacgccca ttcaaacggc ttatgtaaaa aattattacg aagagatggt
     4801 tactttaatg agagagtttt taggagcagg gcttaaggac aattctcatt tggatagagc
     4861 aatattaaca ggtgttttgc gggtatctaa agaaagtatc ttttcaggat taaacaatat
     4921 taaaacatat tcggtactaa atgatcgata cggtgaatat tttggttttg tggaagatga
     4981 ggttagtcga ttattaataa aaacagcttt ggattctcat ataaatgatg taaaggcctg
     5041 gtataatggt taccaaattg gagatgccat tatttataat ccttggtcaa ttgtgaatta
     5101 tattcaagag agaggaaagc taagttcata ttgggttaac acaagtgaca acgcattaat
     5161 taaagatttg tgggttcatt caagtgaact atttaaaggg caatttgaat tactcttaaa
     5221 agatgaacag attataatgc tcatggatga acatctcgca ctgaacaatc tagataaaag
     5281 cgaaattgct atctggacat tattggcgat gtctggatat cttaaagcta attttattaa
     5341 aaacggaaat aatgaatcgc tatattatct aaaaataccc aataaagaag ttaaagattt
     5401 gtataaaaga ttgattgctg agtggctatc gggtgttaac aatgcaatgg tatttaacac
     5461 cttcttaaat aatttattga atggcgagat gaaagatttt gaagacggtt tgcgaagaat
     5521 tctgttgcag acattcagcg cgcatgatat aaaaggtaaa aattctgaga aattttatca
     5581 tggatttatg ttagggcttc tttctggcat agatcaaaat cactataaaa ttgattctaa
     5641 taaagaaagt ggtttagggc gatatgatat tattttaatt ccaaacgata caagtaagct
     5701 tggtattatt ttagaaatta agagtattga tgaaagtagt tcaaaaaacc tgaaagaagc
     5761 tgcaaatgaa gctatcaaac aaattgatga aaaacaatat cacataacgt tgttgcaaca
     5821 tagcattggc atcattgtta ttccagcaag cagttg
//
LOCUS       DAORVT010000022         5801 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_9152_length_5801_cov_3.368604, whole genome shotgun sequence.
ACCESSION   DAORVT010000022 DAORVT010000000
VERSION     DAORVT010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5801
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9152_length_5801_cov_3.368604"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PK389_00825"
     CDS             complement(<1..310)
                     /locus_tag="PK389_00825"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HQY22288.1"
                     /translation="MRYFLPLFLFLVIGGFLWKGLNLNPKEIPSALVGNPAPHFYLGT
                     LENPNHFVSDQIFIGKVTVLNVWATWCVSCKAEHVVWMDLAKKHNLFLVGINYHDDLK
                     L"
     gene            complement(318..2231)
                     /locus_tag="PK389_00830"
     CDS             complement(318..2231)
                     /locus_tag="PK389_00830"
                     /inference="COORDINATES: protein motif:HMM:NF007691.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HQY22289.1"
                     /translation="MIPELGLFSLILAFCLAGLQWSLPWLGLLKHNNFWMQSARPIAL
                     GQSFFISISFLCLLYAFVQNDFSVSYVAYHSNTSLPLVYKISALWGGHEGSLLLWIFL
                     LSTWTTTVSLTTRHLPAPFLARILIVLGMISFGFLLFLLATSNPFVRLLPNYPVDGRD
                     LNPLLQDFGLIIHPPLLYMGYVGFAIPFAFAMSTLWGGDRTLPFAKWMRSWTLLAFSF
                     LTIGIALGSWWAYYELGWGGWWFWDPVENASFMPWIVAIALIHTLMLSDKRQIFQGWT
                     LLLAILVFALSLLGTFLVRSGVLTSVHAFANDPRRGIFLLEFLLVVVSAALTLYSFRA
                     KNFIDNTKVDLISRETAIVISTIFLVVSSAAILLGTLFPLAFDALTNQKLSVGFPYFN
                     AVFIPFIIPVLLLMPLGSMSKWGHNVLGQLLKKLRWIIVSTLFLAVALPLFFAETVPL
                     GVVIGLGLGLWIILGTLKWVFDKIQTKGSVLALSKGAFGMFFSHMGIAVLVIGVTLVS
                     YYQIEREVAITLHQALDILEYKIIFEDLKPIEGPNYVGSRGHFLVFKQGKKEADLFPE
                     KRIFVVQGLRMTETAIDPGFFRDIYISLGEPLKEGGWSVRIYYKPFVRWIWLGALMMA
                     LGGFFSAWGRWLK"
     gene            complement(2228..2689)
                     /gene="ccmE"
                     /locus_tag="PK389_00835"
     CDS             complement(2228..2689)
                     /gene="ccmE"
                     /locus_tag="PK389_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HQY22290.1"
                     /translation="MNTIRKRRLAWVMLLICSSLLAAFFILQALRQNVNLFFTPTEVA
                     TGEAKQKRVIRIGGMVQKGSLVQGKDLNIQFVLTDFAYSVTVQYTGILPDLFKEGQGV
                     VVLGQLDQGQKNLFYADQVLAKHDENYMPPEVGYAIKKGLKNNSITASNQK"
     gene            complement(2826..3578)
                     /locus_tag="PK389_00840"
     CDS             complement(2826..3578)
                     /locus_tag="PK389_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HQY22291.1"
                     /translation="MNRFLHRLGSPQVAYQMAGFCLPWFSIICVLMMIYGVVGGLFLA
                     PPDAEQGDAFRMIYIHVPAAFLSLMIYGIIFVNSVVYWVWHIKVADIVAKCSASVGAT
                     YTMIALFSGALWGKPMWGTWWIWDARLTSELILLFLYLGYMGLRNAIREPKRAAKASA
                     ILAVIGMVDIPIIHFSVEWWATLHQGATLAKFSKPSIAPEMLYPLLAMLGAFILFYAA
                     ILCVKVRLEILKRERKAQWVQEWFRLSRDTAT"
     gene            complement(3583..4251)
                     /gene="ccmB"
                     /locus_tag="PK389_00845"
     CDS             complement(3583..4251)
                     /gene="ccmB"
                     /locus_tag="PK389_00845"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HQY22292.1"
                     /translation="MLKAMQCVIGRDLRLYFQHWGECLIPMLFLILVVSLFPMGLGSH
                     PKLLARIAPAVIWIAVVLSLLMSLESVFRQDYQEGSLAQIVLSPYPLSILLLGKLIAH
                     WMMIGLPLLILAPFLSILLQGSTEGLGVLGLTLVLGIPTLSLIGSIGAALTIGLPNGG
                     ALLAVLVLPIMMPIVIFGTSAVTAATEGVAFYSELYILAALFILALTLGPVALSNAVR
                     ASLA"
     gene            complement(4328..4963)
                     /gene="ccmA"
                     /locus_tag="PK389_00850"
     CDS             complement(4328..4963)
                     /gene="ccmA"
                     /locus_tag="PK389_00850"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF010061.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HQY22293.1"
                     /translation="MLKVSSLGCEWDSRILFSDLTFEVASGSALIVSGANGVGKSSLL
                     KILTGLSLPSSGSISWQDKIFKAGDPYFLSDLLYIGHKIGIQPALTPLQNLEWMIGIS
                     RKSVVKNCRRALEAVDLAAFESVPCEVLSKGQCQRVALARLWLDPPICWILDEPFTAL
                     DETGVSLVQDRFLTHLKHGGILVVATHHKIALNPYPWQEIRLEPVKNNFVF"
     gene            5034..>5801
                     /gene="sohB"
                     /locus_tag="PK389_00855"
     CDS             5034..>5801
                     /gene="sohB"
                     /locus_tag="PK389_00855"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF008745.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HQY22294.1"
                     /translation="MSTFFFEYGLFAAKMITILAITLAAIAGCIILFSTRQQEKESIQ
                     IEKINDKFDNMRDALEETLLSKDEYKALKKDRKKEDKAEEKRIKIQLKKGEQESLRPR
                     LFVLRFDGDMHASEVDSLREAITAILLVAKKEDEVLLILDSSGGLVHNYGLAASELHR
                     IRQRNIPLTVCIDLVAASGGYMMAAVANKIIAAPFAVVGSIGVLAQVPNFFRLLKKHD
                     IDIEQHTAGEYKTTLTMLGENTNKAREKFREELEDTHL"
BASE COUNT         1985 a         1385 c          967 g         1464 t
ORIGIN      
        1 ctaattttaa atcatcgtga taattaatgc ctaccaaaaa tagattatgt ttcttagcca
       61 aatccatcca aactacatgc tctgctttac aactcacaca ccacgttgcc caaacattta
      121 acactgtaac cttcccaata aatatttgat cactgacaaa atgattggga ttttcaaggg
      181 ttcccaaata aaaatgcggc gcgggattac caactaacgc cgatggaatt tctttaggat
      241 ttaaatttaa ccctttccat aaaaaaccgc ctataaccaa gaataaaaac aaaggtaaga
      301 aatatcgcat agtacctcta ttttaaccac cgaccccaag cgctgaaaaa tccacccaac
      361 gccatcataa gagcacctaa ccaaatccat cgcacaaacg gcttgtagta aatacgtaca
      421 gaccagccgc cctctttgag tggctcaccc aaggaaatgt aaatatcccg aaaaaagcct
      481 ggatcaatcg cagtttcagt cattcttaac ccttgtacaa caaatattct cttttctgga
      541 aaaagatctg cttctttctt accctgttta aacaccaaaa aatgccctcg agaaccaaca
      601 taatttgggc cttctatcgg ctttagatct tcaaaaataa ttttatactc taaaatatcg
      661 agcgcttgat gaagtgtgat cgcaacctcg cgttcaatct gataataaga caccagggtc
      721 accccaatca ccaaaacagc tatccccatg tgcgaaaaaa acatgccaaa cgcgcctttt
      781 gataatgcta atacgctacc ttttgtttga attttatcaa acacccattt taaagtgcct
      841 aaaataatcc aaagccctaa tcccaacccg ataactacgc ccagtggtac cgtttctgca
      901 aaaaacaggg gcaatgcaac agctaaaaat aacgtagata caataatcca tcttagtttt
      961 tttaaaagct ggccaagcac attatgaccc catttactca tggaccctaa aggcattaaa
     1021 agtaggacag ggattataaa gggaataaaa actgcattaa aataaggaaa gcccaccgat
     1081 aatttttggt ttgttaatgc atcaaaagcc aagggaaaca aagtgcctaa taatatagct
     1141 gcactactaa caactaaaaa tattgtgctg attacaatcg cggtttctcg agatatcaga
     1201 tcaacttttg tattatctat aaagttttta gccctaaaac tatagagcgt taacgcagcg
     1261 ctcacaacca ccagtaaaaa ctctaataaa aatatgccac gcctgggatc attcgcaaaa
     1321 gcatgtaccg acgttaacac acccgaacgt actaaaaaag tacccaataa acttaaggca
     1381 aataccagaa tcgccaacaa caaggtccaa ccctgaaata tctgccgttt gtcacttagc
     1441 attaacgtgt gaatcaacgc aatagccact atccacggca taaaagaggc attttctaca
     1501 ggatcccaaa accaccaacc tccccatcct aactcgtaat aggcccacca actgcccaaa
     1561 gcaataccga tggttaagaa gctgaatgcg agcaatgtcc aagatcgcat ccattttgca
     1621 aaaggcaagg tgcgatctcc gccccataat gtactcatgg caaaagcaaa aggaatcgca
     1681 aatcccacat aacccatata aagcaacggc ggatgaataa tcaacccaaa atcttgtagc
     1741 aaaggattta aatcacgacc gtctaccgga taattcggca acaatcgcac aaaaggattt
     1801 gaagtcgcta ataaaaataa taaaaaacca aagcttatca tacccaaaac aattaagata
     1861 cgagccaaaa agggtgcagg taaatgccgg gtggttaagc tcactgttgt cgtccaagta
     1921 cttaacaaaa atatccataa caataaagac ccctcatgtc caccccataa cgcgctgatt
     1981 ttatagacta aaggcaaact ggtgtttgaa tgatacgcaa cataactgac agaaaaatca
     2041 ttttgaacaa atgcgtagag taaacataag aagctgattg aaataaaaaa agattgccca
     2101 agtgcaatag gacgcgccga ttgcatccag aaattattgt gcttcaacag ccccaaccat
     2161 ggtaaagacc attgcaatcc tgccaagcag aacgccagaa ttaaagaaaa aagacctaat
     2221 tcgggtatca tttttgattg ctcgccgtta ttgagttatt ttttaaccct ttttttattg
     2281 cataacccac ttcaggtggc atatagtttt catcgtgttt tgccaacacc tgatccgcat
     2341 aaaacaggtt cttttgaccc tgatccagtt gacctaacac aaccacgccc tgcccttctt
     2401 taaagagatc gggcaatatc cctgtatatt gaaccgtcac cgaatacgca aaatcggtta
     2461 gcacaaactg aatatttaaa tctttcccct gcaccaagct gcccttttga accatgccac
     2521 cgattcgaat aacacgcttc tgttttgctt cgcctgttgc cacttcagtc ggtgtaaaaa
     2581 acaaattaac attttggcgt aaagcttgca aaatgaaaaa agcggcgagt aaagagctac
     2641 aaatcaacag cataacccac gctaatcgac gctttctaat cgtattcata acgcattttt
     2701 atctctaaat gattttaata tttttttata cactgttttc cactcttgaa atgcaaaaac
     2761 aacttgtcct cccaaaaggc aaaaagcaaa tatatagcaa gaccaaatat atactgcata
     2821 agagctcatg ttgcagtgtc tctgctaaga cgaaaccatt cttgcaccca ctgtgccttt
     2881 cgttctcgtt ttaaaatttc taaacgaacc ttaacacaca agatcgctgc ataaaataaa
     2941 ataaaagcgc ccaacatcgc caataaggga tacagcattt ctggcgcaat agaaggtttt
     3001 gaaaattttg ccaaggttgc accttgatgt aacgtcgccc accattctac cgaaaaatgt
     3061 ataataggaa tatcgaccat tccaataact gctaaaatag cagaggcttt cgcggctctt
     3121 ttgggttccc gtatggcatt gcgtaatccc atatatccca aatagagaaa taataaaatt
     3181 aattctgagg ttaaacgtgc atcccaaatc caccaagtgc cccacatcgg ttttccccaa
     3241 agtgcaccgg agaataaagc aatcattgta taggttgcgc caacggaagc gctacatttg
     3301 gcaacgatat ccgcaacctt aatatgccac acccaataaa ccactgaatt gacgaaaata
     3361 atgccataaa tcattaaaga taaaaaagca gcaggcacat gaatataaat cattctaaac
     3421 gcatcgccct gttcggcatc cggtggtgct aaaaacaaac cacctacaac gccataaatc
     3481 atcatcagta cacaaatgat gctaaaccaa ggcaaacaaa aacccgccat ttgataagcc
     3541 acttgtggag atcccaatcg atgtaaaaaa cgattcatac cattacgcca aacttgctct
     3601 cactgcattg gataatgcaa cgggccctaa tgttagtgcc aatataaaca acgccgccaa
     3661 tatgtagagt tctgaataaa aagcaacacc ttctgttgct gctgttaccg cactcgtacc
     3721 aaaaataaca atcggcatca taataggcaa aaccaaaacg gccaataatg caccgccatt
     3781 gggtaacccg atggttaaag ctgctccaat gctaccgatt aaactgagcg ttgggatccc
     3841 aagcaccaag gttaacccta aaacccctaa cccttcggtc gacccttgca gcagtatact
     3901 caaaaaaggc gctaaaatta ataagggcag tcctatcatc atccaatgtg caatcaattt
     3961 acccagcaat aaaatagaca acggataagg gcttaataca atttgtgcta agctcccttc
     4021 ctggtaatct tgtctaaata cactttctag agacatgagc aacgataaaa caacggcaat
     4081 ccaaatcacg gctggcgcaa tacgtgctaa taattttgga tgcgatccca accccattgg
     4141 aaacaaactg acaaccaaga tcaaaaaaag catggggatc agacattctc cccaatgttg
     4201 aaaataaagt ctcagatcac gtccaatcac acactgcatg gcttttaaca cagctttgct
     4261 cctcgcctgt caaaaataac tttgtttttt tcgttcgcta aactcgcttc gctcaaataa
     4321 acctcactca aaaaacaaag ttatttttga caggctctag ccgtatctct tgccatggat
     4381 acgggtttag cgcaatttta tggtgtgtag caaccactaa aataccaccg tgcttcaaat
     4441 gcgttaaaaa tcgatcctgc actaaactca cccccgtttc atctaatgct gtaaagggct
     4501 catctaaaat ccaacagatc ggcggatcta accataaacg cgccaatgca acccgttggc
     4561 attgtccctt cgataagact tcgcaaggca ccgattcaaa tgccgctaag tcaacggctt
     4621 ctaatgcacg tctacaattt ttgacaaccg atttacggct aatccctatc atccattcta
     4681 aattttgcaa aggcgttaag gcaggttgaa taccgatttt atgcccgatg tataacaagt
     4741 ctgataaaaa ataaggatct cccgctttaa aaatcttatc ttgccagcta atgctaccgc
     4801 tcgatggcaa cgataagcct gtcaatattt ttaataaact ggatttaccg acgccgttgg
     4861 ctcccgacac aatcaatgcc gaccctgacg caacctcaaa agttagatca ctaaacagta
     4921 ttcggctatc ccattcacag cctaagctgc ttacctttaa cactgcacgc cctcttgcta
     4981 tttatacccg aatcgttaga ctaacacatt caaaagcagc gatggagcac cgtgtgagta
     5041 ctttcttttt cgaatatggt ttatttgctg ccaaaatgat tactattttg gcgattacac
     5101 ttgcggctat cgccggatgt attattttat tctctacaag acaacaagaa aaagaatcta
     5161 ttcaaattga aaaaatcaat gataaatttg ataatatgcg agacgcgcta gaagaaacgc
     5221 ttttatccaa agatgaatat aaagcactga aaaaagacag aaagaaagaa gacaaagccg
     5281 aagaaaagcg gattaaaata caattaaaaa aaggcgaaca ggaatccttg cgtcccaggc
     5341 tgtttgtctt acgatttgat ggcgacatgc atgcttccga agtcgacagc cttcgcgaag
     5401 caattacggc tatcttatta gtcgccaaaa aagaagatga agtattactc atcctcgata
     5461 gctcaggtgg tcttgtacac aattacggtt tagccgcttc agaactccac cgcattcgac
     5521 aacgcaatat ccccttaacc gtttgtatcg atctggttgc tgccagcggc ggttatatga
     5581 tggctgctgt cgcgaataaa attatcgctg cgccttttgc agttgtcggc tctatcggtg
     5641 ttttggcaca agtgcctaat tttttccgtt tgctaaaaaa gcatgatatc gatatagaac
     5701 aacatacagc aggggaatat aaaactacgt tgacaatgct gggtgaaaac accaataaag
     5761 ctcgggaaaa atttcgtgaa gaattagaag atacccatct t
//
LOCUS       DAORVT010000023         5705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_9409_length_5705_cov_3.898761, whole genome shotgun sequence.
ACCESSION   DAORVT010000023 DAORVT010000000
VERSION     DAORVT010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9409_length_5705_cov_3.898761"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..708
                     /locus_tag="PK389_00860"
     CDS             160..708
                     /locus_tag="PK389_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22295.1"
                     /translation="MSKECIYCHSEIHDKAIKCKECDGFQNWRRYLNFSSNILSLLIV
                     LISVLTVSIPVIVDTMRSDVTRIAFNIVEEPKFTAYVENQQDGISGEMFSTVKYNMYF
                     PVVIANSGNKMGFLYRLEASVKDGHGKLDLFSEKENKIKSDSVFTKMITIGLDLKKYD
                     KFLRGWKVSTVDRVRIYVKTLG"
     gene            1083..2027
                     /locus_tag="PK389_00865"
     CDS             1083..2027
                     /locus_tag="PK389_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CBASS oligonucleotide cyclase"
                     /protein_id="HQY22296.1"
                     /translation="MPINNTEFRYYDSNILRLPADKRKEYHEQVDRLINELSKRLQDQ
                     TDIKITKVVKAGSFAKYTILRKTSEDPIDVDVVFYISGHRVDKETLESLSETIYNLLI
                     KLYPSKSVEHFEIQRKAATVKFIKSGLSVDIVPVIEDPSRSGYGWQYDIHDSSKMQTC
                     APCQIKFVRDRKDVDSDFRTLVRLAKKWRNYTELKPLKSFIIELIMAHVLAKNGKDSS
                     IEKRFRDFLLYIAQSELKEVIYFPENGSVYSQFPHPVVIFDPLCNSNNVASRITEKER
                     LEIVDAAKEAWEIANHASSEDDIEIWKELFGPRFRVED"
     gene            2031..2525
                     /locus_tag="PK389_00870"
     CDS             2031..2525
                     /locus_tag="PK389_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22297.1"
                     /translation="MSYSSTATQTYSVNDIETVMRRVTADIVMIASSTGAVSEAKARE
                     WGHDIEVLAKNGYLKAVDLTLFSENSEVKATRYDVNTESGALTTSRPGGVLWPRVNLP
                     YLQIVLIYTQEYNDLARSKMKEKLCVSWVPSSADTSHSTLKSASGRNYVSNAYGMQRE
                     DFSK"
     gene            2522..3418
                     /locus_tag="PK389_00875"
     CDS             2522..3418
                     /locus_tag="PK389_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQY22298.1"
                     /translation="MSQCNIFDSKTILPDSNLATRQKTLLGFDARYEKIHDQLRLLLN
                     LNKLTDWSKKHHGQRIPLCDLVADQYPLVIFYGDVGTGKTATAECIANRLAKESGTED
                     SILFKLSNRVRGSGKVGEMGTLLTQAFQEVVQSAGKNRRAVLIIDEGDSLAASRSQEH
                     SHHEDKVAVNTLIQCIDDLRRLDGRIIVFLCTNRLSVLDAALQRRAAIVEEFLRPNED
                     ERRALLKMDLTGISLTESQIVELVAATGPKNGAPSWTFSDIRTRLYPAALASAFPNQA
                     LNFTHLHDAAKNLRPSPIMEDI"
     gene            3418..4584
                     /locus_tag="PK389_00880"
     CDS             3418..4584
                     /locus_tag="PK389_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011657207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22299.1"
                     /translation="MERSPLFGRRIHIVGSIPKEEKYALAEEVERARQLVETLVKELL
                     RKGATFVVPVDAEKFRESDGLPICFDWLVWDTIEKNLANRPIDAPNPLIVAVKHHKNE
                     EQIPELYQLLWDKFRGSDLVKIESAAHWNMNSKRMEVQARSGDILIAIGGGEGVLFLA
                     NLYHDAGKPVVPLNFKLCPPNTGAQRIYEYALSSSHARRLFQTESETSPHTWINRLDF
                     PNRKDTTERIRDLVALLEDISPPKAFVVRLLNSALPEYPAVQDFFDTVVQPVIEGDLG
                     YKLTVVDGNQAYDYPRIDEEIFAKLHRSSVVIADITGCRPNCFLELGYALGRGLPTIL
                     LAKDGTDHPFDINSFSGHHWKTTGTAEERRREFRKHWEAIKNRPPLVPTEPLIP"
     gene            4581..5132
                     /locus_tag="PK389_00885"
     CDS             4581..5132
                     /locus_tag="PK389_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HQY22300.1"
                     /translation="MNSKREIFISVDVETAGPIPGKYSLLAIGACVVFDDAEIFTCQI
                     KPTTRNAVPEALAVTGLSMEILEQEGLAPAEAMQKFRNWILHVIGSNGTPIFVGFNAA
                     FDWSFINYYFHHYIGENPFGFAALDIKSLYMGFAKCSWKQTKSSQIAASLHPKSSGTH
                     DALQDARYQAELFRLIRPDYSDY"
BASE COUNT         1754 a         1157 c         1196 g         1598 t
ORIGIN      
        1 tgtaacatcc ttggagttct gttaataatt gtacgcttgc aatcccctca ggctcctgac
       61 tcccacttag gaattcttta aatctagttt tgcacttcat gtttgaatcc cccataaaaa
      121 aagaaactat ataatggctt ttgatcaagg gagtaaagaa tgagtaagga atgtatttat
      181 tgtcactccg agatacatga caaagcaatt aagtgcaaag aatgtgatgg atttcaaaat
      241 tggagaagat atttaaattt tagtagtaat atcttgagct tgttgatagt actaatatca
      301 gttttaactg tatcaatacc ggtgattgtt gatacgatga gaagtgatgt gactaggatt
      361 gcttttaata tcgttgagga accaaagttt acagcttatg ttgagaatca acaggatggc
      421 atatcaggtg agatgttttc tactgtcaaa tataacatgt acttcccagt cgtaattgca
      481 aactcaggaa acaagatggg ctttctatat agattagaag cttcagtaaa ggatggtcat
      541 ggaaaattag atctgttttc ggagaaagag aataaaatta aatcagattc agtttttacg
      601 aaaatgatta caattggatt ggatttaaag aaatatgaca agttcttacg agggtggaaa
      661 gtttcaactg tagatcgagt gaggatttac gtgaagactt tgggatagtg aatatatcat
      721 tagcagcaat gaatgaattc ggagaaatat acaaagtcag agagtattct tataacatag
      781 gcaatgtggt agttgctatt caataaatgt ttaagcttaa gccatttaaa aaactctgca
      841 aagctttttg gccactaatt tgatgtattg ctttgcaata agaataaatg ttgagtgaac
      901 ttattttgtg gttaaaaaag catcattaaa attctactga cattattttt cttttggcac
      961 caattttctt cctatttcaa tgttagttta ctagaaccat agaaaaccag atcgtcatct
     1021 acaatacttt tgccattaga ctggataaat aaaaccgaaa aactgaatag ataacaaatt
     1081 aaatgccgat aaacaacacc gaatttcgat attacgatag taatatcttg cgcttgcctg
     1141 ccgacaaacg caaggaatat cacgagcagg tagatcgtct catcaacgaa ctaagtaaaa
     1201 gactgcaaga tcagaccgac atcaaaatta cgaaggtagt aaaggctgga tcgtttgcga
     1261 aatacaccat acttcgtaaa acttcggaag atcccataga tgtcgatgtg gttttctata
     1321 tctctggcca cagggtcgat aaggaaaccc tcgaaagctt gagtgaaaca atttataatt
     1381 tgctgattaa gctttatccc agcaagtctg ttgaacactt cgaaattcag cgcaaggcag
     1441 cgaccgtcaa attcatcaaa tcgggcttga gcgttgatat cgttcccgta attgaagatc
     1501 catccagatc aggttacgga tggcaatacg atatacacga tagctccaaa atgcaaacgt
     1561 gcgccccttg ccaaatcaag tttgtacgtg atcgcaagga tgttgatagt gatttccgga
     1621 cattggtgcg cctagccaaa aaatggagaa attacaccga gcttaagcca ctaaaatcct
     1681 ttatcatcga attgattatg gcgcatgtgc ttgcaaaaaa cggcaaggac agctcgattg
     1741 agaaacgctt tcgcgatttt ctgctttaca tcgctcaatc tgaattgaaa gaggtaattt
     1801 attttccgga aaacggatcg gtatattcac agtttcccca tcccgtcgtc atttttgatc
     1861 ccttatgcaa cagtaacaac gtcgctagtc gcatcacgga aaaagagcga ttggagattg
     1921 tggatgccgc aaaagaagct tgggaaatcg ctaaccatgc ctcttctgaa gacgacattg
     1981 aaatatggaa ggaacttttt ggtcctcgat tcagagtgga ggattgagtt atgagttatt
     2041 caagcaccgc gactcaaact tactcagtta atgatattga aaccgtaatg cgaagagtca
     2101 ctgccgacat cgtcatgata gcgagcagca caggtgctgt ttccgaagca aaagcgcgcg
     2161 aatgggggca tgatattgaa gttcttgcaa aaaacggata tttaaaagca gtggacttga
     2221 cattatttag cgaaaacagc gaagtaaaag caacacgata tgacgttaac acagaatctg
     2281 gcgcattaac cacaagccga cctggaggcg ttctttggcc gcgtgtgaac ctaccttatc
     2341 tccaaatagt tctgatatac actcaggaat acaatgattt agcgcgatcc aaaatgaaag
     2401 aaaaactttg cgtctcttgg gttccctcca gcgcagacac aagccattca acactcaaat
     2461 ccgcttcagg tagaaactat gtcagtaatg catatggcat gcagcgtgag gacttcagca
     2521 aatgagtcaa tgtaatattt tcgatagtaa aacgattctc ccagacagca atttggcaac
     2581 acggcagaaa acgctacttg gatttgatgc tcgttacgag aaaattcatg atcaactgcg
     2641 cttattgctc aaccttaata aactgactga ttggagcaaa aagcatcatg ggcaacgcat
     2701 tcctctttgc gatcttgtag ctgaccaata tccgttggtt atcttttatg gcgacgtagg
     2761 tactggaaaa acggcgacgg ccgagtgtat tgccaatcgc ttagcaaaag aatctggcac
     2821 tgaggactct atactattca aattgagcaa cagagtgcgc ggaagtggaa aagtgggtga
     2881 aatgggcacc ttattgaccc aagcattcca agaagtagtt caatcagcag gaaagaacag
     2941 acgtgcagta ttgattattg atgaagggga ctccctggct gcttctcgtt ctcaggagca
     3001 tagtcatcat gaagacaagg ttgcagtcaa tacattaata caatgcatag atgacttgcg
     3061 taggcttgat ggaaggataa tcgtatttct ctgcacgaac cggctttctg tgcttgatgc
     3121 tgcactacaa agacgagcag cgatagtaga agagttcctt cgaccaaatg aagatgaacg
     3181 acgggcttta ttgaaaatgg atctgactgg aatttccctt accgagagtc aaattgtcga
     3241 attagtagct gctaccgggc caaaaaatgg agcacctagc tggacttttt cagacattcg
     3301 aacccggtta tatcctgcag cattggcttc ggctttccct aatcaagcgc ttaacttcac
     3361 acatcttcat gatgcagcaa aaaatttgcg tccatcacca atcatggagg atatctgata
     3421 gaaagatcac cgcttttcgg ccgtagaatt catattgtag ggagtattcc gaaagaggaa
     3481 aagtacgcgt tagccgagga agtagagcgt gcccgccaac tggtagaaac cttggttaag
     3541 gaattgctcc gtaagggtgc tacttttgtt gttccggtgg atgctgagaa atttcgggaa
     3601 tccgatggtc ttccaatctg cttcgattgg cttgtctggg ataccatcga aaagaatttg
     3661 gcgaatcgtc caatcgacgc accaaatccg ctcattgtcg ccgtcaagca ccacaagaac
     3721 gaagagcaaa ttcccgagct atatcagctc ctatgggata agttccgtgg atcggatcta
     3781 gtcaagatcg agagcgcggc ccattggaac atgaatagca aacggatgga ggttcaagca
     3841 cgttctggtg atattctgat tgcgattggc ggcggtgaag gagtgttatt tttagcgaac
     3901 ctctatcacg atgcgggaaa acctgttgtc cccttgaatt tcaaactttg tccacctaac
     3961 accggagcac agaggatcta cgaatatgct ctgtcaagca gtcatgcacg gcgccttttt
     4021 caaacagaaa gtgagacttc accgcacacc tggatcaacc gacttgattt ccctaaccgt
     4081 aaagatacta ccgaaagaat acgagacttg gttgccctac ttgaagatat ttcgccaccc
     4141 aaggcattcg tagttcggct gcttaattca gctcttccgg aatatcctgc cgttcaggac
     4201 tttttcgaca ctgttgttca gccggtgatc gaaggagatc ttggctataa acttaccgtt
     4261 gtagacggaa accaagcata tgattacccc agaatcgatg aagaaatttt cgctaaactg
     4321 catcgcagca gcgttgttat tgctgacatc actggctgcc gcccaaattg cttcctagaa
     4381 ttgggatatg cacttgggcg aggcctccca actatactcc ttgcaaaaga tggaactgac
     4441 catccttttg acattaactc gttttccggt catcactgga aaacgacagg aaccgcagaa
     4501 gaacgtcgaa gggaatttcg caaacactgg gaagcgatta aaaatcgccc tcccttagta
     4561 cctacggagc cccttatacc atgaattcta aacgtgaaat ttttatatct gtggatgtag
     4621 agactgccgg ccccattcca ggaaaataca gcttgctagc aatcggggct tgtgtcgttt
     4681 ttgatgatgc agaaatattc acttgtcaga tcaagccaac aactcgtaat gcagttccag
     4741 aggctttggc agtaacaggg ctctcaatgg agatattaga acaagaaggc ctagcacctg
     4801 ctgaggccat gcaaaaattt cgcaactgga ttttacacgt aatcggttcc aacggtacgc
     4861 ccatattcgt aggctttaat gctgcatttg actggtcttt cattaactac tatttccacc
     4921 attacattgg cgaaaatccc tttggttttg ctgccttgga cattaaatcg ttatacatgg
     4981 gatttgccaa gtgctcatgg aagcaaacca aatcaagcca gatcgccgcc agtctccatc
     5041 caaaatcaag cggcactcac gacgcactcc aagacgcgcg ttatcaagcg gagttattcc
     5101 gtttaattcg acctgactac tccgattatt aattagcaga tcgtgaaaaa attgtttttg
     5161 agatcggctg aagtgaataa ttattcaata acatcctttt tagcttaagt acttattgat
     5221 tcatatgtgg ctggttcgat agtcaattcc gctgaaagat accatattga tgtttgttgt
     5281 acttaaactg atgtccatat gatgtaccta caaaacttta gtcgaatatc gaaattagat
     5341 gcaattcttt gattttatag cgctgctatg atggctctaa tctatgatcc actaattacg
     5401 aaattaatta actgaataat caatttctca acaacatttt tatctgtatt taatttctat
     5461 gtgctgtcca catagcgtct acatgaataa gatatattta ttaatcgtat tataactatt
     5521 tgtttttatg gtgccgacac caagaatcga actctggacc ttctgaaaat cagacatcag
     5581 ttcattttcc aattaagaaa accataaatc aaatactatt tctaaagctt gtttgatcga
     5641 ttgcaaatat tcgctctcaa gcctaccttg ctttttgcca attcgagaga tatcaactgc
     5701 acgta
//
LOCUS       DAORVT010000024         5676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_9504_length_5676_cov_4.500978, whole genome shotgun sequence.
ACCESSION   DAORVT010000024 DAORVT010000000
VERSION     DAORVT010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5676
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9504_length_5676_cov_4.500978"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="PK389_00890"
     CDS             <1..717
                     /locus_tag="PK389_00890"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001204.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HQY22301.1"
                     /translation="GDLVNRGPQSLETLRFIKNLGSHAITVLGNHDLTLLAVAFNAIS
                     YDPKRYTFGDILAASDRDALIDWLRHRPFIHQDSSLGYTLVHAGLPPQWDLVLAKSLA
                     KEVELILQSENPLLFFENLFGNMPNHWDPTLTGMDRLRFIVNCFTRLRFCNVAGELEL
                     KVKESAENAPAGYLPWFSINTRKSQDLKILFGHWASLEGKCREPNVFPLDTGCVWGNC
                     LTAMRLSDGQRFTEPCQPPT"
     gene            complement(721..1518)
                     /locus_tag="PK389_00895"
     CDS             complement(721..1518)
                     /locus_tag="PK389_00895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020158993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQY22302.1"
                     /translation="MIAFHRIVLVNILDKGQNMPLLSVIIITKNEAHNIEDCLKSVAW
                     ADEIVVFDSGSTDNTVDICRRYTDKVFITDWPGYGAQKQRALEAATGEWVLSIDADER
                     VMPQLKAEILSQIPHTSYDAFEITFSSEYCQKVIRFGDWWHDTQAVLFRRNQARFISL
                     LAHERIEIQGNIGKLKSNIHHLAYPNLDLVLKKMNDYSSLSAKQKQTEGAKGSVLKAV
                     FRGLWTFMRGYIFRLGFLDGKEGFLLAISNAEGTYYKYIKLMYLNKS"
     gene            1646..2449
                     /gene="coxB"
                     /locus_tag="PK389_00900"
     CDS             1646..2449
                     /gene="coxB"
                     /locus_tag="PK389_00900"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HQY22303.1"
                     /translation="MSSSWLSTLLFVLLWASSLPAGADYTFNMTPGITEISRGIHHLH
                     MTIFWICVAIGTGVFGVMFYAIIWHRKSQGVTAAQFHENTMVEIIWTAIPFTILILMA
                     IPAAQLLLKMHNTAESDLSIKIVGHQWYWEYEYLGEDVKIISRLATPQDQINNRDIKG
                     QHYLREVDNPIVVPEKKKIRFLTTSNDVIHAWWMPALGIKRDAIPGFINEAWANIDHT
                     GTYRGQCAELCGALHGFMPIVLEVKTEAEYQTWLSAKKAENIKPTPHTE"
     gene            2456..4042
                     /gene="ctaD"
                     /locus_tag="PK389_00905"
     CDS             2456..4042
                     /gene="ctaD"
                     /locus_tag="PK389_00905"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HQY22304.1"
                     /translation="MSSSVEKHFFDTEHHRPHKGILRWVFTTNHKDIGTLYLWLSFVM
                     FLVGGAMAMCIRAELFKPGLQFFDPGFYNQLTTLHGLIMIFGAVMPAFVGLANWMLPM
                     MIGAPDMALPRMNNWSFWILPFAFTLLLSTLFMSGPAPDFGWTAYAPLSTTYGPKSTD
                     FFIFSIHLMGISSIMGAINIIATILNCRAPDMTLMKMPIFVWTWLITAFLLISVMPVL
                     AGAVTMMLMDRHFGTSFFDAAGGGDPVMYQHIFWFFGHPEVYIMILPSFGILSEIIPT
                     FSRKRLFGYSSMVYATSSIAFLSFLVWAHHMFTTGLPVVGEIYFMLATMLIAVPTGVK
                     IFNWVATMFRGALSFEAPMLFCVAFLIMFTMGGFSGLMLAMVPADYQYHDTYFVVAHF
                     HYVLVPGAIFGIIAAVYYWLPKWTGHMYSETLAKFHFWLSAISINVLFFPMHFLGLAG
                     MPRRIPDYALEFADFNRIASVGGFVFGASQLLFLYIVIKTIRGGAKAPAQVWEGAQGL
                     EWTLSSPPPYHSFITPPSVH"
     gene            4056..4598
                     /locus_tag="PK389_00910"
     CDS             4056..4598
                     /locus_tag="PK389_00910"
                     /inference="COORDINATES: protein motif:HMM:NF003465.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HQY22305.1"
                     /translation="MEKPHKTLIYRLFFIVVLMFGFTFALVPIYNVFCDVTGLNGKVD
                     TKKPGKFVRYKNENALKADRLIVLELDINHHQNIRCEFTAEKPALQVIPGELTHTIYH
                     VKNLTDKKMIIQAIPSISPGFLAKHLKKLECFCFEQQTLEPFESRDLPLRFWLETEIP
                     EDIHRMTLSYTLFDVTESKK"
     gene            4595..5467
                     /locus_tag="PK389_00915"
     CDS             4595..5467
                     /locus_tag="PK389_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HQY22306.1"
                     /translation="MTDDYQRYYVPEQSYWPIIGSIGLFTMIIGGINVLHEHDGWLIF
                     LAGSFVTVFMMFGWFGSVIKESRSGLYSPQLDRSFRWGMIWFIFSEVMFFAAFFGALF
                     YARHFSVPWMGGIGAKPATHALLWDHFRALWPLLQNPDPTKFLGPFEAMGAWGLPALN
                     TVILLSSGATVTFAHLALKNNHRKKLILGLIATVILGILFLSFQAYEYYHAYTKLGLK
                     LNSGIYGSTFFMLTGFHGAHVTIGTIMLIVILVRCIRGHFTPEKHFGFEATAWYWHFV
                     DVVWLMLFIFVYWL"
     gene            complement(5455..>5676)
                     /locus_tag="PK389_00920"
     CDS             complement(5455..>5676)
                     /locus_tag="PK389_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017157720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HQY22307.1"
                     /translation="PSTLATVLIFTMLFCILASLGAGLYYLVKDQGKTKRIVRALSVR
                     IILSLVLFAALLIAFAMGWIAPHGLLQSQ"
BASE COUNT         1558 a         1196 c         1132 g         1790 t
ORIGIN      
        1 ggcgatctgg ttaatcgcgg accgcaatct ttagaaacac tgcgctttat taaaaattta
       61 ggctctcatg ccatcaccgt tttaggaaat catgatttaa cgctcctcgc agtcgctttt
      121 aatgcgattt cttatgatcc caaacgatat actttcggtg atatcttagc agcatctgac
      181 cgagatgctt taatagattg gttacgacat cggcctttca ttcaccagga ttccagttta
      241 ggatacacat tggttcacgc gggactcccc cctcaatggg atctcgtact cgcaaaatca
      301 cttgcaaaag aagtcgaatt gattctgcaa agcgaaaatc cactactatt ttttgaaaat
      361 ttgtttggaa acatgcctaa tcattgggat ccgacattaa caggtatgga tcgactgcgc
      421 tttattgtta attgctttac acggttacga ttttgtaatg ttgcaggtga attagagctg
      481 aaagtcaaag aatctgccga aaatgcccca gcgggttatt tgccatggtt ttctattaat
      541 acacgcaaaa gtcaggattt aaaaatccta tttggacatt gggcctcgct agaaggcaaa
      601 tgtagagaac ccaatgtttt tccgctagat acgggttgcg tgtggggaaa ttgcttaact
      661 gccatgcgtc tatcagatgg tcagagattt actgagccat gccaacctcc aacctagagc
      721 ttaactttta tttaaataca tcaactttat gtacttgtaa taggtgccct cggcattcga
      781 aatagctaat aaaaaacctt ctttgccatc taaaaacccg agtctaaata tatagcctcg
      841 cataaaagtc caaagcccac gaaaaaccgc ttttaaaaca ctaccctttg caccctcggt
      901 ctgtttttgc tttgcgctta aactggaata atcattcatt ttttttaaga ccaaatccaa
      961 attaggataa gcaagatgat gaatattgct ttttaatttt ccaatattgc cttgtatttc
     1021 tattctttca tgcgctaata aagaaataaa acgtgcttga tttcgtcgaa ataaaacagc
     1081 ttgggtatca tgccaccaat caccaaatcg aatcactttt tgacaatatt ctgaactaaa
     1141 agtaatttca aaagcatcat aagacgtatg ggggatctgt gacaatattt ctgccttcag
     1201 ctgtggcata acgcgctcat ccgcatctat cgataatacc cattctccag tggcggcttc
     1261 taatgcacgc tgtttttgcg ccccataccc cggccaatcg gtaataaata ctttatccgt
     1321 atatcgccta caaatatcaa cggtattatc tgtgctaccc gaatcgaaca caacaatctc
     1381 gtccgcccac gcaaccgatt ttaggcaatc ttctatatta tgtgcctcgt ttttggtaat
     1441 aatgataacg cttaataacg gcatattttg acctttatct aaaatattga ctaaaacaat
     1501 ccgatggaac gctatcatat attacaaaat cttgcccaac aacgaaagct gctaacaaat
     1561 cggctaaaac ttagttgacg atactataat atccaaggtt caatagcctt ctatatcctg
     1621 acatttaaga catggagagg tatcaatgtc ctcatcttgg ttaagcaccc tgcttttcgt
     1681 gcttttatgg gcgagtagcc ttcccgcagg ggcagattac acgtttaata tgacgccagg
     1741 cattactgag atcagtcggg gaatccacca tttgcatatg accattttct ggatctgtgt
     1801 tgcgattggt accggtgtat ttggcgttat gttttatgcg attatttggc accgtaaatc
     1861 tcaaggtgta acagccgcgc aattccatga aaacaccatg gtagaaatca tttggacagc
     1921 gatccctttt accatcctga ttttaatggc gattcccgca gcacaattat tactgaaaat
     1981 gcacaatacc gcagaatcgg atttatccat caagattgtg ggacatcaat ggtattggga
     2041 atacgaatac ctcggcgaag atgtgaaaat catcagccga cttgccaccc cccaagatca
     2101 aataaataat agagatatta agggacagca ctacctgagg gaagtcgaca accctatagt
     2161 tgtacccgaa aaaaagaaaa ttcgcttttt aacaacttca aatgatgtga tccacgcctg
     2221 gtggatgccc gccttgggta ttaagcgcga tgccattcct ggatttatta acgaagcctg
     2281 ggcgaatatt gatcacaccg gcacctaccg agggcaatgt gccgaattat gcggagcact
     2341 ccacggattt atgcccatcg tgctagaagt caaaacagaa gcagaatatc aaacctggct
     2401 tagcgcaaaa aaagctgaaa atataaaacc aaccccccat acagagtaat acaccatgag
     2461 tagcagcgta gaaaaacatt ttttcgatac agaacatcac cgtccacata agggcatact
     2521 gcgctgggta ttcacaacaa atcacaaaga tattggtaca ctctatttat ggttaagctt
     2581 tgtcatgttc ttagtgggtg gcgccatggc catgtgcatt cgcgcagaac tttttaaacc
     2641 cggactacag ttttttgatc ccggatttta taatcaatta accacattac acggcttaat
     2701 tatgattttt ggcgccgtta tgccagcctt cgtcggatta gcaaactgga tgctacctat
     2761 gatgattggt gcgcccgaca tggcattgcc ccgcatgaac aactggtctt tttggatatt
     2821 accctttgct tttacgttgt tattaagcac attatttatg agcggcccgg cacccgattt
     2881 tggctggaca gcctacgcgc ctctctctac cacttatggt cctaaaagta ccgatttctt
     2941 tattttttcg atacatttga tggggatttc ttccattatg ggtgccatca atattatcgc
     3001 aaccatttta aattgccgcg cacccgacat gactttaatg aaaatgccta tatttgtctg
     3061 gacctggttg attacggcat ttttgttaat ttcagtaatg cctgtgctgg cgggagctgt
     3121 cacaatgatg ttaatggata gacattttgg caccagcttt tttgatgcag ccggcggtgg
     3181 ggatcccgtg atgtatcaac atatattttg gttttttggt catcccgaag tgtacattat
     3241 gattttgcca agctttggca ttctgtctga aattatcccc acttttagcc gaaaacgatt
     3301 atttggctac agctctatgg tatacgccac cagttctatt gcatttttat catttctcgt
     3361 ttgggctcac catatgttta cgacagggct accggttgtc ggtgaaattt actttatgtt
     3421 ggcaaccatg ttaatcgctg ttccaacggg cgttaaaatt tttaattggg tcgccacgat
     3481 gtttcgcggt gcattgagtt ttgaagcccc catgcttttt tgtgttgcat ttttaattat
     3541 gtttaccatg ggcggttttt cgggcctgat gcttgccatg gtgcctgccg attatcaata
     3601 tcacgatact tactttgtgg ttgcacattt ccactacgtt ttggtacctg gcgccatttt
     3661 tggaatcatt gccgcggtat attattggct gccaaaatgg acaggacata tgtatagcga
     3721 aactttggca aaatttcatt tttggctatc ggctatttct attaatgtct tattttttcc
     3781 catgcatttt ttgggtttag ctggcatgcc aagacggatc cccgactatg ccttagaatt
     3841 tgcagatttt aatcgcattg caagtgtcgg cggttttgtg tttggcgctt ctcagctttt
     3901 gtttttatat attgtgatta aaacgattcg aggcggtgca aaggcacccg cacaggtgtg
     3961 ggaaggtgcg cagggtctag aatggacctt atcttcgcca ccaccttacc atagttttat
     4021 aacaccccct agcgtgcatt aatggacgat cgataatgga aaaaccccac aaaacactta
     4081 tctatcgact gttttttatt gttgtgctga tgttcggttt tacatttgca ttagtcccga
     4141 tttataacgt tttttgcgat gtaacaggcc ttaacggcaa agtcgataca aaaaaaccgg
     4201 ggaaatttgt acgttacaaa aacgaaaatg ctttgaaagc tgatcgcctt attgtgcttg
     4261 aattggatat taatcaccac caaaatatac gttgcgaatt cacagcagaa aagcccgcac
     4321 ttcaggtgat ccccggcgag ctcacacata ccatatacca tgttaaaaat ttaactgata
     4381 aaaaaatgat tattcaggcc atcccgagca ttagtcccgg atttttagca aaacatttaa
     4441 aaaaactaga atgtttttgt tttgaacagc aaacgctgga gccttttgaa tcacgcgatt
     4501 tacctttgcg tttttggtta gagacggaaa ttccagaaga tattcatcga atgactttgt
     4561 cttatacttt atttgatgtc acggagtcaa aaaaatgaca gacgattatc aacgctacta
     4621 tgtgcccgaa caaagttatt ggcccattat cggttccatt ggtcttttta ccatgattat
     4681 cggtgggatt aatgtattgc acgagcatga cggttggctc atttttttag caggttcttt
     4741 tgtaactgtt tttatgatgt ttggatggtt tggcagtgtg atcaaagaaa gtcgcagtgg
     4801 actctacagc ccacaactgg atcgttcgtt tcgctgggga atgatttggt ttattttttc
     4861 tgaggtgatg ttttttgctg ctttttttgg tgcacttttt tatgccagac atttttcggt
     4921 tccttggatg ggtggtattg gcgcgaaacc cgcaactcat gcgttattat gggatcactt
     4981 tagagcactg tggcccttat tacaaaatcc tgaccccacg aaatttttag ggccttttga
     5041 agccatgggc gcttggggac tcccagcttt aaatacagtg atcttattgt cttctggtgc
     5101 aacggttact tttgcacatc ttgcccttaa aaataatcat cgaaaaaaat taatcctcgg
     5161 attaatagca acggttattt taggcatttt atttttatcc tttcaagcct atgaatatta
     5221 tcatgcttat acaaagcttg gacttaaatt aaattcgggc atttacggtt cgacgttttt
     5281 catgttaacg ggttttcatg gcgcacatgt caccattggt acaattatgc tgatcgtaat
     5341 tttggtgcgc tgtatacgcg gacactttac acccgaaaag catttcggat tcgaagcgac
     5401 tgcctggtat tggcactttg tcgatgttgt atggttaatg ttgtttattt ttgtttattg
     5461 gctttgaagt aatccatggg gagctatcca acccatcgcg aatgcaatca ataatgcagc
     5521 aaacaaaaca agggagagaa taatacgcac ggaaagcgct ctgactattc gcttcgtttt
     5581 accttgatct ttgactaaat aatacaaacc agcacctaaa ctcgctaaaa tgcaaaatag
     5641 catggtaaat atcaatacag ttgctaatgt agatgg
//
LOCUS       DAORVT010000025         5630 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_9653_length_5630_cov_3.360179, whole genome shotgun sequence.
ACCESSION   DAORVT010000025 DAORVT010000000
VERSION     DAORVT010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5630
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9653_length_5630_cov_3.360179"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2575
                     /locus_tag="PK389_00925"
     CDS             <1..2575
                     /locus_tag="PK389_00925"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HQY22308.1"
                     /translation="RERKQTAVRELIDAYRLLGHLHADIDPLKRREKVEVPELMLSYY
                     HLTSADLSLTFDAGSLPGMSTRTLQQILDDVKRCYCGTIASEFMHIPDSPERIWVQQQ
                     VEAIGLQNRVSLDVQQRILEYLIAADGLEKYLGAKYPGAKRFSLEGTDSLLVALDTFI
                     QQGGVSGAKEIIICMTHRGRLNVLVNLLGKIPNQLFDEFEGKHNDIKLETGDVKYHQG
                     FSSDVSTPGGNVHLSLEFNPSHLEIVTPVVCGSVKARQERRKDNDHTEVLCIALHGDA
                     AFSGQGVVMETLNMSQTRGFKIGGTVHIVVNNQIGFTTSNPNDIRSTLYCSDVGKMIE
                     VPIFHVNADDPEAVYTVMTLALAYREKFKKDVLVDLMGYRRQGHNEADEPAVTQPVMY
                     ELIRKMPTARQLYADKLIAEKTLSTTEIDNMIKAYRDTLDSRQQAVARNLVNELKRDF
                     SSHWELYVSKDWRTPVNTGVALTTLKKLAESQTTLPEGFVLHPRVQKIIEDRRKMTAG
                     ELPLDWGYAETLAYATLLNEDYLVRLSGQDVGRGTFFHRHVVLHDQRDNAEYMPLSHL
                     SKNQGKFTVVDSLLSEDAVLAFEYGFAKTEPRGLTIWEAQFGDFANNAQVVIDQFISS
                     GEQKWGRLCGLTLYLPHGYEGQGAEHSSARLERYLQLCAEHNMQVCVPTTPAQVFHMI
                     RRQMVRPIRKPLIVLTPKSLLRHKLAVSCLQELETGAFFPVLPEVDEVSPEQVKRVVL
                     CSGKVYYDVLEKRRANNQKEVVILRVEQLYPFPETELKKQLDLYPAALVVVWCQEEPK
                     NQGAWYNIQHHLVAIITQQQQLQYAGRQAAAAPAVGYLHLHQEQQAALVNDALNKGTT
                     V"
     gene            2572..3795
                     /gene="odhB"
                     /locus_tag="PK389_00930"
     CDS             2572..3795
                     /gene="odhB"
                     /locus_tag="PK389_00930"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HQY22309.1"
                     /translation="MSMEIKVPVLPESVADGSIATWYKKVGEKIKRDEHLLDIETDKV
                     VLEVVAPADGFLESILKKEGDTVKSEEVVGLFTAEVVSISEKPSVEKPPVEALISVEQ
                     TSKPQQPLSPAVRQLVKEQSVNLTEVQGTGKDGRITKADVTKIVSQKEAVSKPPSPSS
                     AKKAETSPLELPMGERVEKRVPMSRLRARMAERLLQAQQSAAILTTFNEINMQPVMGL
                     RQKYKETFEKDYGVRLGFMSFFTIAVVEALKRFPIVNASIDGNDVIYHGYHDIGIAVS
                     SPRGLVVPLIRNAELLDMAQIEKKIAEFKEKAQNGQLTIEEISGGTYTISNAGGFGSL
                     MSTPILNPPQSAILGMHKIEERPIVENGIVVIRPMMYVAMSYDHRIIDGTDAVQFLVT
                     VKELIEDPARLLLNV"
     gene            3819..4988
                     /gene="sucC"
                     /locus_tag="PK389_00935"
     CDS             3819..4988
                     /gene="sucC"
                     /locus_tag="PK389_00935"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011398504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HQY22310.1"
                     /translation="MNLHEYQSKQLLAKAGLPVSKGVVAMSVEQALLAVDGLSTKSWV
                     VKAQVHAGGRGKAGGVKQVKNKDELATVARGLLHARLVTAQTNEKGQPINELLIEETC
                     EIDRELYLGAVIDRSLKKIVFMASTEGGVEIEKVAHESPDKILKFPVDPLLGVMPYQC
                     RELASKLQLSEVAAKQLVKIITGLYKLFQEYDLSLIEINPLVLTKQGDLICLDAKLNV
                     DDNALYRQQALREMRDITQEDAREIVAREWELNYIPLEGAIGCMVNGAGLAMATMDLI
                     KLYGGEPANFLDVGGGATEERVKEAFKIILSDSRVKAVLVNIFGGIVRCDFIAQGVIK
                     AVSEVGIQVPVVVRLEGNHAFEASQILEKSGLNIIAAKSLGEAAEKVVSAAGGKK"
     gene            4985..>5630
                     /gene="sucD"
                     /locus_tag="PK389_00940"
     CDS             4985..>5630
                     /gene="sucD"
                     /locus_tag="PK389_00940"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004385340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HQY22311.1"
                     /translation="MSILIDKNTKVICQGFTGQQGTFHSEQAIEYGTRMVGGVTPGKG
                     GQTHLGLPIFDTVREAIHVTEADASVIYVPAPFCKDSIIEAIDAGIRLVVCITEGMPV
                     LDMLQVKHYIRDKAVQVIGPNCPGVITPDACKIGIMPGNIHMSGRVGVVSRSGTLTYE
                     AVKQTTDVGLGQSTCVGIGGDPIPGSSFVDILALFNEDSQTEAIVMVGEIGGSAE"
BASE COUNT         1709 a          972 c         1372 g         1577 t
ORIGIN      
        1 ccgagagcga aaacaaaccg cggtacgtga actcattgat gcctatcgat tattgggcca
       61 tctgcatgcg gatattgatc ccttaaaacg gcgtgaaaaa gtagaggttc ctgaattaat
      121 gctttcgtat tatcatttaa catctgcgga tctttcttta acatttgatg caggatcact
      181 gcccgggatg tccacacgga cattgcaaca aattttggat gatgtaaaac ggtgttactg
      241 tggtacgatt gcttcagaat ttatgcatat tccagattcg cctgagcgaa tttgggtgca
      301 gcagcaggtt gaagcgattg ggcttcaaaa tagagtatcg ttagatgttc aacaacggat
      361 attagaatat ctgatagctg ccgatgggtt agaaaaatat ttgggtgcaa aatatccagg
      421 tgcgaaacgt ttttcattag aaggcacaga tagtctattg gtggcattag atacttttat
      481 tcaacaaggt ggcgtgagcg gtgctaaaga aattattatt tgcatgacgc atcgcggtcg
      541 attaaatgtt ttagttaacc ttttaggaaa aatacctaat caattgtttg atgaatttga
      601 aggtaagcat aatgatatta aattagaaac gggtgatgta aaataccacc aagggttttc
      661 gtcggatgta tcaacgcctg gtggtaatgt acatttgtct ttagagttta atccctcaca
      721 tttagagatt gttacaccgg tcgtttgtgg ttctgttaag gccaggcaag aacgtagaaa
      781 agataatgac cacacggaag tgttatgcat tgcgcttcat ggagatgctg ctttttcagg
      841 ccaaggtgtc gttatggaaa ctttaaatat gtcgcaaacg cgcgggttta aaattggcgg
      901 aaccgtgcat attgttgtca ataatcaaat cgggtttaca accagtaatc caaatgatat
      961 tcgatcaact ttatattgtt cagatgttgg taaaatgatt gaagtaccaa tttttcatgt
     1021 gaatgcggat gatcccgaag ctgtgtatac cgtgatgaca ttggctttag cgtacagaga
     1081 aaaatttaaa aaagatgtgc ttgtcgattt aatgggctac cgtcgacaag gacacaatga
     1141 agcggatgaa cccgctgtca cgcaaccggt gatgtacgaa ttgattcgca aaatgccaac
     1201 ggctcgccag ttatatgcag ataaactgat cgcggagaaa acgttatcaa ccacagaaat
     1261 tgataatatg ataaaagcct atcgcgacac cttagatagc cgacaacaag cggttgcacg
     1321 taacttggtt aatgaactca agcgcgattt ttcgagtcat tgggagctgt atgtttccaa
     1381 agattggcga acgccggtca acacgggtgt tgctttaaca acactgaaaa aattggcaga
     1441 aagccaaact acattgcccg aagggtttgt tttacatccg cgcgttcaaa aaattattga
     1501 agatcggcga aaaatgacag cgggagaact accgttagat tgggggtatg cggaaacgtt
     1561 agcatacgcc actttgttaa atgaagatta tttagtgaga ttgtcggggc aagatgtggg
     1621 tcgcgggaca ttttttcatc gacatgtggt tttgcatgat caacgcgata atgcagaata
     1681 tatgccatta tcgcaccttt ctaaaaatca gggcaagttt acagtggttg attcgttatt
     1741 gtcggaagat gcggtacttg cgtttgaata tgggtttgca aaaacagaac ctcgtggctt
     1801 gactatttgg gaagcgcaat tcggggattt tgcaaataat gcacaagtgg tgattgatca
     1861 atttatcagt tccggcgaac aaaagtgggg caggctgtgt ggtttaacct tgtatttgcc
     1921 acatgggtat gaagggcaag gtgcagagca ttcgtctgca aggctcgagc gatatttgca
     1981 actttgtgca gaacataata tgcaggtgtg tgtgccaacc actcctgcac aagtttttca
     2041 tatgatccgt cgtcaaatgg tacgtcctat ccgaaaaccg ttaattgtac tgacgcccaa
     2101 aagtttattg cgccataaat tagcggtttc ttgtttacaa gaattagaaa caggtgcatt
     2161 tttcccggtc ttaccagagg tagatgaggt ttcgcctgaa caagtaaaac gtgttgtttt
     2221 atgcagtggt aaagtttatt atgatgtact agaaaaaaga cgcgcaaaca accaaaaaga
     2281 ggttgtaatt ctacgtgttg aacagttata tccttttcct gaaaccgagc taaaaaaaca
     2341 gctcgatttg tatcctgccg ctttggttgt tgtttggtgt caagaagaac ccaaaaacca
     2401 aggcgcttgg tacaatattc aacatcattt agttgctatt attacgcaac aacaacagtt
     2461 acaatatgcc ggaagacaag ctgcggcagc gccagctgtt ggctatttac atttacatca
     2521 agaacagcaa gctgcattag taaacgatgc gttaaataaa ggaacaactg tatgagtatg
     2581 gaaattaaag tacccgtatt accagaatct gtcgcagatg gttctattgc cacttggtat
     2641 aaaaaagtgg gtgaaaaaat taagcgtgat gaacatttgt tagatattga aactgataaa
     2701 gtggtactag aggtagtagc gcccgcagat ggatttttag aaagcatttt aaaaaaagaa
     2761 ggcgacactg ttaagtcaga agaagtcgtt ggattattta ctgcggaagt tgtttcaata
     2821 tcagaaaagc catcagtaga aaaaccgcca gtcgaagcac taatttcagt cgaacaaacc
     2881 tctaaaccac aacaaccctt atcaccagcg gtgcggcaat tggttaaaga acaaagtgtg
     2941 aatctcacag aagtgcaagg aacaggaaaa gacggtcgca ttaccaaagc agatgtcaca
     3001 aaaatcgttt ctcaaaaaga agctgtttct aagccacctt cgccttcttc agccaaaaaa
     3061 gcggaaacaa gtcccctaga attgccaatg ggcgaaagag ttgaaaaacg cgtgcccatg
     3121 tctcgcctac gtgcacgtat ggccgaacga ttgttgcaag cgcagcaatc agccgcgatt
     3181 ttaaccacct ttaatgaaat taatatgcag cccgtcatgg gattaaggca aaaatacaaa
     3241 gaaacctttg agaaagatta tggcgtgcgc ttgggtttta tgtctttctt taccattgct
     3301 gtcgtagaag ccttaaaacg ttttccgatc gtgaatgcgt ctatagatgg caatgatgtt
     3361 atttatcatg gttaccacga tattggtata gcggtgtctt ctccaagagg gttggtcgta
     3421 cccttaattc gaaatgcaga attattagat atggcacaaa ttgaaaaaaa aattgctgag
     3481 tttaaagaaa aggctcaaaa tggccagcta accattgaag aaatttcggg tggaacctac
     3541 accatttcta atgcaggggg ttttgggtcg cttatgtcta ccccgatttt aaatccacca
     3601 caaagtgcga tattggggat gcataaaatt gaagagcgac cgattgtcga aaatggcatt
     3661 gttgtgattc gacccatgat gtacgttgct atgtcttatg atcaccgcat tattgatggc
     3721 acggatgccg ttcaattttt agtgacggtt aaagaactca ttgaagatcc ggcgcggttg
     3781 ttgttaaatg tttaatcttc ttatacagga tagccattat gaatttgcat gaatatcaat
     3841 caaaacagtt gctagcgaaa gccggattac cggtttctaa aggtgttgtt gcgatgagtg
     3901 tagagcaagc cttattagcg gtcgatggct tgagtaccaa aagttgggtt gttaaagcac
     3961 aggttcatgc tggggggcgt ggaaaagcgg ggggtgttaa acaggtaaaa aataaagacg
     4021 aattagcgac tgttgcgcga ggccttttgc atgcacgatt ggttactgca caaacaaatg
     4081 aaaaaggtca gcccattaat gagttattaa ttgaagaaac gtgtgaaata gacagagagc
     4141 tgtatttagg tgctgtcatc gatcgcagct taaagaaaat tgtattcatg gcgtctacag
     4201 aaggcggtgt agaaatcgaa aaagtggcac atgagtcacc cgataaaatt ttaaaatttc
     4261 ctgtagatcc tttgttaggg gtgatgccgt atcagtgtcg ggaattagcg tctaaattgc
     4321 aactgagcga ggtagcagct aagcagttgg ttaaaataat taccggttta tacaagctgt
     4381 ttcaagaata cgatttaagt ctcattgaaa tcaacccgtt ggtgttaaca aaacaaggtg
     4441 atctgatctg tttagatgca aagcttaatg ttgatgataa cgccttatat agacaacagg
     4501 cattgcgtga aatgcgcgat attacccaag aagatgcacg cgaaatagta gcgagagagt
     4561 gggaactcaa ttatattccg ttggaaggtg ctattggttg catggtaaat ggtgccgggc
     4621 ttgcgatggc aacaatggac ctgattaaat tatatggtgg agaacccgct aattttttag
     4681 atgtgggtgg aggcgcaacg gaagagcgtg ttaaagaagc ttttaagatt attttatccg
     4741 atagccgtgt aaaggctgtg ctagttaata tttttggtgg cattgtacga tgtgatttta
     4801 ttgcgcaagg ggtcatcaaa gccgtgtcgg aagtgggtat tcaagtgcct gtggttgtaa
     4861 gattagaagg caatcatgca ttcgaagcca gtcaaatact cgaaaaaagt ggactcaata
     4921 ttattgcggc taaatcactc ggagaagctg cagaaaaagt ggtttcggct gcgggaggca
     4981 aaaaatgagt attttaatcg ataaaaatac taaagtgatt tgtcaggggt ttaccggaca
     5041 gcaaggtaca tttcattcag aacaagcgat tgaatatggt acgcggatgg tgggtggggt
     5101 tacgccaggt aaaggaggtc aaacgcattt aggtttaccg atttttgata cggtgcgtga
     5161 agctatacac gtcacagaag ctgatgctag tgttatttat gtgcccgcac ccttttgtaa
     5221 agattctatt attgaagcga tcgatgcggg gatccgttta gtggtttgta ttaccgaagg
     5281 tatgcctgtc ttagatatgt tgcaagtgaa acattatatt cgtgataagg ccgtgcaagt
     5341 cataggacct aattgtccag gtgtgataac gccggatgct tgtaaaattg gcattatgcc
     5401 aggaaacatt catatgtctg gtcgagtagg tgttgtatct cgttctggaa ctctgacata
     5461 cgaggcggtt aaacaaacga cagatgttgg cttaggacaa agcacatgtg ttggcattgg
     5521 cggagatcct attccgggaa gtagttttgt ggatatttta gcgctcttta atgaagattc
     5581 acaaaccgaa gcgattgtga tggttggtga aattggaggg agtgcagaag
//
LOCUS       DAORVT010000026         5586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_9771_length_5586_cov_3.221117, whole genome shotgun sequence.
ACCESSION   DAORVT010000026 DAORVT010000000
VERSION     DAORVT010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9771_length_5586_cov_3.221117"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..891)
                     /locus_tag="PK389_00945"
     CDS             complement(22..891)
                     /locus_tag="PK389_00945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HQY22312.1"
                     /translation="MVYSMTAYASKNVSLSGGFIARWEIRSTNHRYLEVSFKLPPLQG
                     DLEYRLRDNLRKIITRGKVDCFLKLDAVENNKRDLNIDFNLVEQLVVASQKISARFPV
                     NADVAVFDILRMPSVVSLPETDLSALEISLEESFLTALQDLKETRAREGEKLGGYIEE
                     RLVKMHERLKSITNRLPEIEKAMRIKFYEKVHDLQVETDKSRFDQVVANLIQKMDISE
                     ELDRLKAHLTEVERVLKDEKVMGRRLDFLMQELNRESNTVGSKAIDTLTVQDAITFKV
                     LIEEMREQIQNIE"
     gene            complement(893..2107)
                     /locus_tag="PK389_00950"
     CDS             complement(893..2107)
                     /locus_tag="PK389_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HQY22313.1"
                     /translation="MQLKQNYYLFLILVTLMSSVSIFATDTYLPALPDMALHFNCTQT
                     EIQLSFTVFLLGLAGSQLIVGALSDRFGRKKIVISGFVLFTLSSILCANAHTLSQFII
                     FRLLQAIGGGVGSVTSRALVVDRYNRQESVKIFSTIFPIVGSSAAIAPFIGGYLTTFF
                     GWRSNFFMIAIFGLIILFCVFLCLKNKVPSASTKHPPTSKKQGYSDVLGNLEFLGYAL
                     IIAAGFCVFRSYSVETPFVFSRQGYAPEKMGQFYIALAVAYIVGNLFAKKLINKWTVE
                     YVLRVGFSFFIVGGLCMVSSLLIFDNNPYGLIVPMAVITFGNGLLFPVASAAAMTSVR
                     SEHYGIASGLLGAIQCVLAAFCSNWVGELCQGRAVSLSLFIGTLILVGFLSYLLLIVY
                     KPKSDVSLTEDI"
     gene            2229..3014
                     /locus_tag="PK389_00955"
     CDS             2229..3014
                     /locus_tag="PK389_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007228001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HQY22314.1"
                     /translation="MLRIISANVNGIRSAAKKNFFSWLSKQNTDIICLQETRAEVHLL
                     TDPVFFPQGYYTYFCSAEKKGYSGVAIYTRIKPDQVHQDGLGWKEADTEGRYIQADFK
                     NLSVASIYFPSGTSGEARQAIKYDFMAQYEKKLRAQLKKGREYILCGDWNIAHKNIDI
                     KNWRTNQKTSGFLPEERAWLDKVFDTIGYVDAFRVVNQEPDQYTWWSQRSPTARENNV
                     GWRIDYHVVTPGLIKKILSAHIYKEEFFSDHAPLIIDYDLTLK"
     gene            3177..3962
                     /locus_tag="PK389_00960"
     CDS             3177..3962
                     /locus_tag="PK389_00960"
                     /inference="COORDINATES: protein motif:HMM:NF022472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2608 domain-containing protein"
                     /protein_id="HQY22315.1"
                     /translation="MNHYAKMCFLAILIALSTKEVTAEMITVNRMNDVKITVEQLLQQ
                     YQGKEILTAFDIDMTLTQPNNPATFYPALIKHYAIYKKIMGSLTPEQKDLTATLTVFL
                     PVRLVEAETPQIVSAIQDRGVKTIAFTAMLTGKLKDKNQTHNKIEALRYNALNSLGIN
                     FANNFTEKEIYFNDLPSHNNSYPAFYNGILFTNGEQGKNGKGSVLIKFIQRTSMSLKA
                     IVLIDDKKKNLEDVEQFLKNHHPAIKFIGIEYQDTVLTVNPIN"
     gene            complement(4312..5277)
                     /locus_tag="PK389_00965"
     CDS             complement(4312..5277)
                     /locus_tag="PK389_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012460827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HQY22316.1"
                     /translation="MSNTDVLGVDISKATFDVALLANDKVKTKKFNNKNKGFLELIEW
                     LKNKNITTLHVCMEATGNYGDALATYLFELGYTVSVVNPAQIKGFGKSELSRNKTDRA
                     DAQLIARFCRAMAPVSWTPKPQHIRELQAWVRRLEALQNMYYQENNRFGVVDVIVKAS
                     IKKIMGQLEKEIADIKKTIREHIGAHPDLQAKSALLATIPGVGEATISQILAFVGNVE
                     AFKNAKQLAAFVGLNPTQRQSGTSVRGRTHLSKMGDSNLRKALYLPAMTAKKYNPIIK
                     KFCDNLANGGKPKMLIIGAAMRKLIHIIYGVLKSQKPFDANIAMV"
BASE COUNT         1770 a         1250 c          971 g         1595 t
ORIGIN      
        1 caaataggtt tcctcttgct actattcgat attttgaatt tgttcccgca tttcttcaat
       61 aagtacttta aatgtgatag catcttgaac agtcaaagta tcaattgctt tggagcctac
      121 cgtgttagat tctcggttta attcttgcat taaaaaatct aatcgacgac ccataacttt
      181 ctcatccttc aaaacccttt ccacttctgt taaatgcgct tttaatcgat ctaactcttc
      241 tgaaatatcc attttctgaa tgaggttagc gaccacttga tcaaaacgac ttttgtcagt
      301 ttcaacttgt aagtcatgca ctttttcata aaattttatc cgcattgctt tttcgatttc
      361 tgggagccta ttcgttatcg acttcaatcg ttcatgcatt ttcactaaac gctcctcgat
      421 atatccacct aacttttcgc cctctcttgc acgggtttct tttaaatcct gcaatgccgt
      481 cagaaaagat tcttccaaag aaatttccaa ggctgacaaa tctgtctccg gcaaagaaac
      541 gactgaaggc atgcgcaaaa tatcaaaaac agcgacatct gcgttaacag ggaatcgcgc
      601 gctgattttt tgactggcca ccaccaattg ctctaccaaa ttaaaatcaa tattcaagtc
      661 gcgcttatta ttttctactg catccaactt taaaaaacaa tctactttcc cacgggtaat
      721 aattttacga agattgtcac gcaaccgata ttctaaatct ccctgcaaag gtggcaattt
      781 aaaagagact tccagatatc gatggtttgt cgatcggatc tcccagcgtg caataaaacc
      841 accggacaat gatacatttt tactcgcata ggcagtcatg ctataaacca tgtcagatat
      901 cctccgttaa gctaacatct gatttgggtt tataaacaat caataataaa tagcttaaaa
      961 atccaaccaa aataagcgtt ccaataaaca acgacagcga aactgcgcga ccctgacaca
     1021 gctctcctac ccaattgcta caaaatgcgg ccaacacaca ctgtatcgca ccgagcaatc
     1081 cagaagcaat accataatgc tccgatcgca cagatgtcat ggcggctgct gatgccaccg
     1141 gaaataaaag tccattacca aatgtaatca ctgccatggg cacaataagc ccataagggt
     1201 tgttgtcaaa aatgagtaac gaactcacca tgcacaaacc gcctacaata aaaaaggaaa
     1261 aacccactcg tagtacatat tctactgtcc atttgttaat cagtttcttg gcaaataaat
     1321 tgcccacgat ataagcaacc gcaagcgcga tataaaattg tcccattttt tcaggcgcat
     1381 aaccttgcct actaaataca aaaggagtct ctacagaata gctgcgaaac acacaaaatc
     1441 ctgcagcaat aattaaggca taccccaaaa attccaggtt acctaacaca tccgaatacc
     1501 cctgtttttt cgatgtcggt ggatgtttag tggatgcact cggcacttta tttttaaggc
     1561 acaaaaatac gcaaaacaat atgattaacc caaaaattgc aatcatgaaa aaattggatc
     1621 gccatccaaa aaaggtggtg agatagcctc ctataaacgg cgcaatcgcc gcagaagagc
     1681 ctacaatcgg aaatatcgtt gaaaaaatct taaccgattc ttgtctgttg tatctatcta
     1741 caaccaatgc gcggcttgta acagaaccta caccaccccc aatagcttgc agtaaacgaa
     1801 atataataaa ttgtgacaac gtatgtgcat tcgcacacaa aatagaagac agagtgaata
     1861 gaacaaaacc tgaaatcacg attttttttc gaccaaaccg atcggacaaa gcgccgacga
     1921 ttaactgact gcctgccaaa cctaacaaaa acactgtaaa acttaattgg atttctgttt
     1981 gtgtacaatt aaaatgcagt gccatatcgg gcaacgccgg taaataggta tcagttgcaa
     2041 aaatactcac agacgacatc aacgtaacga ggataagaaa aagataataa ttttgcttga
     2101 gctgcacgat ttattccatt aaaatacttt agatataatg aatcattgta ggttgactat
     2161 cacacagtgt caactgatat gtttgctccc cttatctaca ttcatatcca gtcgtaatga
     2221 gcttaagtat gcttagaatt atatccgcta acgtcaatgg catccgatct gctgctaaaa
     2281 agaatttttt tagctggcta tctaaacaga ataccgatat catttgcctg caagaaaccc
     2341 gcgccgaagt ccatctctta accgacccgg tcttcttccc ccaaggctat tacacctatt
     2401 tttgcagtgc cgaaaaaaaa ggctatagcg gcgtcgcgat atatactcgc attaagcccg
     2461 atcaagtaca tcaagacgga ttagggtgga aagaagccga tactgaaggg cgttatatac
     2521 aggccgattt taaaaatctc agcgttgctt ctatctattt cccatcggga accagcggag
     2581 aagctcgcca agccattaaa tatgacttta tggcacaata cgaaaaaaaa ttacgtgcac
     2641 aattaaaaaa agggcgtgaa tacattctct gtggcgattg gaatatcgca cacaaaaaca
     2701 tcgatataaa aaattggcga acaaaccaga aaacttcagg ttttttacca gaagaacgtg
     2761 cttggttaga taaagtgttt gacactatcg gctatgtcga tgcttttaga gtggtgaatc
     2821 aagaacccga tcaatatact tggtggtctc agcgttctcc gactgcgcgc gaaaacaatg
     2881 tgggttggcg tattgattac cacgtggtta cgccgggact gataaaaaaa attctctctg
     2941 cgcatattta caaagaagaa tttttttctg atcacgcgcc gttaattatt gattatgatt
     3001 taacgttaaa ataaagccaa tgaggccaaa tctagactcc gtacagatta tgtatacagt
     3061 acggagtcta gatttgaccc gattgctttt taagctggct agcgttaaaa ttagtgcaaa
     3121 aaacacgatc ccaacgtatt atgtataatt ttacatctaa tagggatact ctcaaaatga
     3181 atcattatgc aaaaatgtgt tttctagcga tattaattgc tttatctacc aaggaagtta
     3241 ctgctgaaat gataactgtt aacagaatga atgacgttaa aattacagtc gagcagttac
     3301 tgcaacagta tcaaggtaaa gagatattaa cagcgtttga tatagatatg acactgacgc
     3361 aaccaaacaa cccagcaact ttttaccctg ctctcatcaa gcattatgct atttacaaga
     3421 aaattatggg cagcttgacc cctgaacaaa aggatttaac agccacatta acagtattct
     3481 tgcctgtaag gcttgttgaa gctgagacac cacaaatcgt ctctgctata caagatcgcg
     3541 gtgtaaagac tatcgctttc accgcgatgc ttaccggcaa attaaaagac aagaatcaaa
     3601 ctcacaacaa aattgaagca ttaagataca acgctctaaa tagccttgga ataaattttg
     3661 caaataattt tacagaaaaa gaaatttatt tcaacgatct tccatcccac aataatagtt
     3721 atcctgcatt ttataatgga atattattca caaatggtga gcaaggcaaa aatggcaagg
     3781 gctctgtttt gattaagttc atacagcgaa catcgatgtc tcttaaagct atcgttctaa
     3841 tagatgataa aaagaaaaat cttgaagatg tcgagcaatt tcttaaaaac catcaccctg
     3901 ctatcaagtt cataggcata gaataccaag ataccgtctt gactgtcaac cccataaatt
     3961 aaaaaatttt cattgtagga ttttatagat gcgttcaaag ccgcattcat ggcggcataa
     4021 tggtaggctt cccgtcaagc ggggtcaagg agctttccct cgcggactcg ggaactcctc
     4081 cttgaccccg ccttccgctt cgcctattgc gctgcctatg ccatgaatga catccttcaa
     4141 tgctaacctt gcaattaaaa aataaaaaaa ttcataaaac aactcagcga aagatttctt
     4201 gttaaaaaaa ctttcagcaa cactctaccc tcgtcggctc tcgcaatttt agcttgccgg
     4261 tcgctcacga tagggttata tttcaatcat cgcttaacgg cggactttta tttaaaccat
     4321 cgcaatattc gcatcaaaag gtttttgaga ctttaataca ccgtagataa tgtggattaa
     4381 ttttctcatg gccgcaccta taattagcat ttttggcttt ccaccattag ctaagttatc
     4441 gcaaaacttc ttaatgatgg gattatattt cttagcggtc atggccggca gatatagtgc
     4501 ttttcttaga tttgaatcac ccatttttga taggtgtgta cgaccgcgta ctgaagttcc
     4561 agactgccgt tgtgtaggat tgagaccaac aaaggctgcc aactgtttag catttttaaa
     4621 tgcctctaca ttacctacaa aagccaatat ctgagaaatt gttgcttcgc caacgccagg
     4681 aattgttgct aataatgcac tttttgcctg taaatctgga tgcgcgccaa tatgctcacg
     4741 aatcgttttt ttaatgtccg caatttcttt ctctagctgc cccatgattt ttttaataga
     4801 tgcttttaca atgacatcta caaccccaaa acgattattc tcttggtaat acatgttctg
     4861 taatgcctca agacgacgca cccaagcttg taattctcga atgtgttgag gttttggagt
     4921 ccaggagact ggagccattg cacgacaaaa acgggcaatt aactgtgcat cagctcgatc
     4981 cgttttgttg cgagatagtt cactttttcc aaaccctttg atttgcgcag gattaaccac
     5041 actaaccgta taacccagct cgaacaaata ggttgccaat gcatcaccat aattaccagt
     5101 agcttccata caaacatgta aagttgttat gtttttattt tttagccatt caatcagttc
     5161 caaaaaccct ttgtttttat tattaaattt tttagttttt actttatcat ttgccaataa
     5221 agcgacatcg aaagtggctt tactgatatc cacacccaat acatccgtat ttgacatatt
     5281 cgcctcctct taaatagtat ttcttagcgc ttggcgagat tttgttcaag accatcctcg
     5341 tttatacagg ctcatcgagt aaaatcgagg cctaagacac cgtccagtct tacagaacaa
     5401 aagtagggac gagttgctaa atcttgatat cagggtcgtt tagccctgag gagcttcacg
     5461 gcttcctcgt cccctcaccc aagtgccccc ggatgattta atcactctcc agtgattgag
     5521 aacaagatac gaggagcttc cctcgcaggc tcgggactcc tccttgacac agcccgccgc
     5581 ttcgcc
//
LOCUS       DAORVT010000027         5530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_9937_length_5530_cov_3.500274, whole genome shotgun sequence.
ACCESSION   DAORVT010000027 DAORVT010000000
VERSION     DAORVT010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9937_length_5530_cov_3.500274"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1781)
                     /locus_tag="PK389_00970"
     CDS             complement(<1..1781)
                     /locus_tag="PK389_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22317.1"
                     /translation="MQSSEQLISELNAPEVKFHLEAPQDLEAPQASPVQVNPQEHLAD
                     IQVPPIHVNPEAFRELLKSLTLPKYHLRGLEKYNPELVQQYRTFRQETYPGLNRQDIQ
                     FLPLLNEIATELLGFIEACNNQLVFKGQASSSTLTGIANELSRFTGSNQLTSIWFFSV
                     LFRTAAALKEASAEELEVFRHAAEGHNNPELIERLFKYRDHEWGADAWSHADALMAIA
                     NTGSRTNIGLSLEVSKKLEEQLDAKFPTQVYYPIMGENKLGITLLLSLYLEQVGLLGL
                     SDKPADAHGVSMSPLIFMAHDLFHIQNNRKSQMDAVFSYAGHAICAHVKESGIADLAV
                     EPVVSIAVQKHITLAHTFQTVLQHFLTSLYPERGLDALNKAIAGFFYSVHEVPKCVSA
                     NTYLCSELEEILNKLCDAGIEHVSSEQSWESPADPFCTSPETGEILLSKEDQTALMQK
                     LTRQRLQQERNSLYFAGIPVEYYSKSDSEKLEYLESLITDVTLENNGRFIDLKITFRD
                     GKTLYDSMPTLFHKWHNADDAIGLLKLARIRIKKPNLQEEAKSLEEQRQLVIQTLIKV
                     QDGLKETINFFRQEALHYATREEPETG"
     gene            complement(2339..3142)
                     /gene="cysQ"
                     /locus_tag="PK389_00975"
     CDS             complement(2339..3142)
                     /gene="cysQ"
                     /locus_tag="PK389_00975"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HQY22318.1"
                     /translation="MLYQDWIPKILSVALEAAEAIVDIYDRNAYQVKKKLDTSPVTEA
                     DLCAHQIIQKGLFSIDPTIPFLSEEGDTTPYEIRSHWSRYWLVDPLDGTLEFIKGSGE
                     FTVNIALIENHSSVLGVVVVPVHRHTYWAIQGEGAYAKLASADPVRIHTNHFPKGSLK
                     VAASRRLNQNTKPGWLALMNRLNQPELLYCGSAWKICLVASGEVDLYPQFGTTSEWDT
                     AAGQCILEAAGGQLVDFVGRPLQYNAKPSLKNSAFCAISDSGLIPICCG"
     gene            3261..3851
                     /locus_tag="PK389_00980"
     CDS             3261..3851
                     /locus_tag="PK389_00980"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HQY22319.1"
                     /translation="MDQKFEFFSDDYVFEGGSPQSDSTRELIVRLDKWLWAARFFKTR
                     AVARLAVEKGNVFYNGERAKPSREIELGATLIIQQGRCQKTVIVRGLSTRRRGTEESI
                     QLFEETEESRLNREQASVFQPDYTQHPSQFQPQLFYPSTPTERTGNYNTVQGEQQRER
                     HAIRFLRRSFNKGESRTPRTTVTHPNYSQADSENLD"
     gene            3852..>5530
                     /gene="priA"
                     /locus_tag="PK389_00985"
     CDS             3852..>5530
                     /gene="priA"
                     /locus_tag="PK389_00985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HQY22320.1"
                     /translation="MNIILRIAIPVPLRQLFDYLPPIEPHAVKLEPGMRVLVPFGRRK
                     TVGIIVEITDHTDHPIEKLKHIQACLDQEPLFPKTILSLIQWASRYYQGALGEVFEIA
                     IPKRLRQEPKELIPKEITLNTKKQAHKKSKKQSTSSLISTTISPDLTLNPAQEKAINS
                     ITHHFGQFKTFVLDGVTGSGKTEVYIRVVQAALHAGKQALLLVPEIGLTPQLLSRFQE
                     RFAVPIAALNSSLTDKQRFLAFENARTGAAPIVIGTRLAAFVPLKNPGVFIIDEEHDA
                     SFKQQEGFRYHARDLLILRGSLEHCPVVLGSATASLETLHNIQAGRFHPLSLPHRAGN
                     AIFPTLQILDVRHKKCVEGLSSELIVAMQEHIANKGQVLLFLNRRGYAPVLMCFACDW
                     VATCKNCDAKMTLHQRSETLQCHHCELTIPLYTECPCCKTKNPKPVGIGTERLEAALQ
                     ENFPTQSIVRIDRDTTRKKGELKKAIDLIQEGKADILIGTQMIAKGHHFPNVTLVGIL
                     DIDHALFSTDFRSIEKMGQLITQVAGRSGRAERPGHVILQTCHPEHPLLKQ"
BASE COUNT         1647 a         1217 c         1092 g         1574 t
ORIGIN      
        1 atacccgttt caggctcctc cctagttgca taatgcaaag cttcttgcct aaaaaagtta
       61 atcgtttctt taagcccatc ttgcaccttt attaaggttt gtataaccag ctgtctttgc
      121 tcttcaaggg acttagcttc ttcttgtaaa ttgggttttt tgatgcggat cctcgccagt
      181 tttaacaaac cgatcgcgtc atctgcattg tgccatttgt gaaagagcgt gggcatcgaa
      241 tcatacaaag ttttgccatc tctaaaagta attttcaaat caatgaatct tccattattt
      301 tcaagagtga catctgtaat caatgactct aaatactcga gcttttccga atccgacttg
      361 ctgtaatact caacaggaat gcctgcaaag tataagctat ttctttcctg ttgcaaccgc
      421 tgtctcgtta atttttgcat aagcgcggtt tgatcttctt tgcttaataa aatttcaccc
      481 gtttctggag aagtgcaaaa tggatctgct ggagactccc aagattgttc agaactcaca
      541 tgctcgatcc ccgcatcaca taatttattg agtatttctt ctaactccga gcacaggtaa
      601 gtgtttgcac taacgcattt cggcacttca tgtactgaat agaaaaatcc agcaatggct
      661 ttattcaagg cgtctaatcc tctctctgga tacaaggatg taagaaaatg ttgtaagacc
      721 gtttggaagg tatgagccaa tgtaatgtgt ttctgaaccg caatagaaac aacaggttct
      781 actgccaaat ctgcaattcc agattcttta acatgtgcgc aaattgcatg tccggcatag
      841 gaaaaaacag catccatttg agactttcgg ttattttgta tatgaaacaa atcgtgcgcc
      901 ataaaaatta atgggctcat agacaccccg tgtgcatccg caggcttatc acttaatcct
      961 aacaaaccca cttgttctaa atagagtgat aacaacaatg taatgcccag tttattttcc
     1021 cccatgattg ggtaatagac ttgagttgga aattttgcat ccaattgctc ttccagtttt
     1081 ttagacacct caagagatag cccaatgtta gttcttgagc ccgtattggc aatagccatc
     1141 agcgcatcag catgactcca tgcatccgct ccccattcat gatctctata cttaaaaagc
     1201 cgttctataa gttcaggatt gttatgtcct tctgctgcgt gtcgaaatac ctcgagttcc
     1261 tctgcagagg cttccttcaa cgccgctgca gtgcggaata agacgctaaa aaaccaaata
     1321 gatgtcagtt gattagaacc cgtaaaacga gataactcat ttgcaattcc agttaatgta
     1381 cttgaagagg cttgtccctt aaagaccagt tgattattgc aagcttcaat gaagccaagt
     1441 aattccgttg caatttcatt taacaggggt agaaattgaa tatcctggcg atttaaccct
     1501 gggtatgttt cttgtctgaa agtgcgatat tgttgtacaa gctcagggtt gtacttctca
     1561 agccctctaa gatgatattt ggggagagtt aaacttttta agagttcccg aaacgcttct
     1621 ggattgacgt gtattggcgg aacctgaata tccgctaaat gctcttgtgg attgacttgt
     1681 acgggtgaag cttggggcgc ttctaaatct tggggcgctt ctaaatgaaa cttaacttct
     1741 ggcgcattta gttcggatat taattgttct gaagattgca tgtaatgtcc cctaacatat
     1801 aagcataata atttgcaact gtatcagaaa cgtcccgcga taaaaagatc taagtttcaa
     1861 agcttgttag ctgaaaattt gttagttaga ttatttgctt taaggctggg tgctacaaaa
     1921 actatccaca aagttatcca cagatattct ggataaaaac aagactcatc gacagcccgg
     1981 aatttaaagt tatttggtta tccaaaactt tattagactg gataacaaaa gttattcaca
     2041 agctacaaag cgacttagac ggtcgttatc caacccatgt tagtcgggta aaaaatgtta
     2101 aggtacggaa tttgttaaaa atttacttta aaaatcatat agataaaaaa taatacctct
     2161 atagagacct attttacgtt ttacagcttg ggttagacga caaatataat atttaggtta
     2221 tgcacagagt tatccacaag ttaaacaacc gattgaccca gcatatccaa ttttattgat
     2281 cttttattga cctcaaaatt tttaacaagt gatttctatt tttgaaggtc aaactcattt
     2341 atccacagca aattggaata agtccgctat ccgaaatagc gcaaaatgct gaatttttta
     2401 gggaaggctt tgcattatac tgtaacggtc tacctacaaa atcaacgagt tgtccacccg
     2461 cggcttccaa aatacactgg cctgcggctg tatcccattc gcttgtcgtg ccaaattgcg
     2521 gataaagatc aacttctccc gatgctacca aacaaatttt ccaagcgctg ccacagtaca
     2581 acaactcggg ttggtttaat ctgttcatta atgccaacca accgggtttg gtattttgat
     2641 ttaatcgccg gctcgctgcc accttcaaag aacccttggg aaaatgattc gtatgaatgc
     2701 gtacaggatc ggcagaagcc agtttggcat acgctccttc accctgaatc gcccaatagg
     2761 tatgtcggtg caccggaaca accacaaccc ccaagaccga cgagtgattc tcgattaagg
     2821 caatattgac cgtgaactct ccgctgcctt taataaattc cagcgtacca tctaagggat
     2881 cgacgagcca ataacgagac caatgcgaac ggatctcgta aggtgtcgta tcaccttctt
     2941 cagagagaaa tggtattgtt ggatcaatgg agaatagtcc tttctgaata atctggtggg
     3001 cgcataaatc cgcttcggtc acaggtgaag tatctaactt tttctttacc tgataggcgt
     3061 tgcgatcgta aatatcgaca atcgcttccg ctgcctcaag tgctacggac aatattttgg
     3121 gtatccaatc ttgatataac atgcatacct agtatatcac cttcggttca gttgactaaa
     3181 aaaatttaat tcgcgctgcc aattttcaat tggttctgct atgatgcaat tccaaattta
     3241 ctacaatagt gttaacaaga atggatcaaa aattcgaatt tttttcagat gactacgttt
     3301 ttgaaggtgg atctccacaa tcagacagta cacgcgagct cattgtacgc ttagataaat
     3361 ggctctgggc agctcgattt tttaaaaccc gtgcggtcgc aaggctggca gtggaaaaag
     3421 gaaatgtctt ttacaatggc gaacgtgcca aacccagtcg agaaatagag cttggtgcaa
     3481 cgctcattat tcagcaaggt cgatgccaaa agacggttat tgtccgcgga ctctctaccc
     3541 gtcgacgcgg tacagaagaa tccatacagc tgtttgaaga aacggaagaa agccgcctca
     3601 atagagaaca agcatctgtc tttcaacctg attacacgca gcatccctca caatttcaac
     3661 cgcagttgtt ttatccttca acgcctacag aaagaaccgg taattacaat acagtgcaag
     3721 gtgaacaaca gagagaacgc catgccattc gatttctacg ccgctctttc aataaaggtg
     3781 aatcccgcac accacgaacc acagtcactc atcccaacta ttcacaggca gatagcgaga
     3841 atcttgattg agtgaacatc atcctccgca tcgccattcc tgttccttta cgacaattat
     3901 ttgattattt gccaccgata gaaccccatg cagtaaaatt agaacccggc atgcgtgtac
     3961 tcgttccttt cggacgccgg aaaacggtgg ggatcattgt agaaatcact gatcacacgg
     4021 atcaccccat tgaaaagctg aaacatatac aggcttgctt agatcaagaa ccccttttcc
     4081 caaaaacaat tttatcgctg atccaatggg cgagccgata ttatcaaggt gccttaggcg
     4141 aagtgtttga aatagcgata cctaaaagac tgcgacaaga acctaaggag ctaatcccta
     4201 aagaaataac gctcaacaca aaaaaacaag ctcacaaaaa atctaaaaaa caatcgacgt
     4261 cctctctcat ctctacgaca atatctcctg atttaacgct gaatcccgct caagaaaaag
     4321 ccatcaatag catcacacat cattttggac aatttaaaac atttgtgctg gacggcgtga
     4381 cgggtagtgg caaaacagaa gtgtatattc gtgtggttca agccgctttg catgcgggaa
     4441 aacaggcgtt attgctcgta cctgaaatcg gattaacccc acaattattg tcgcgttttc
     4501 aagaacgatt tgctgttcct attgcggctt taaattcgtc tttaactgac aagcaacgtt
     4561 ttctggcttt tgaaaatgca agaacgggtg cggcacccat tgtgattggc acgcgcttag
     4621 ccgcttttgt gccactcaaa aatccgggag tttttattat tgatgaagaa catgatgcct
     4681 cgtttaaaca acaggaaggc tttcgatacc atgcgcgcga tttgcttatc ttgcgcggat
     4741 cactcgaaca ctgtccggtt gttttaggga gtgcaacagc ttcgcttgaa acactacata
     4801 atatccaggc agggcgtttt catccactgt ctttaccaca ccgtgcaggt aacgccattt
     4861 tcccaacgct gcaaattcta gatgtgcgtc ataaaaaatg tgtagaagga ttatcttctg
     4921 aactgattgt agcgatgcaa gaacatattg ccaacaaagg tcaagtctta ctctttttga
     4981 ataggcgtgg ctatgcgcct gtgttaatgt gttttgcctg cgattgggtg gcaacctgca
     5041 aaaattgcga tgcaaaaatg acgctacacc agcgttcgga aacgctacaa tgtcatcact
     5101 gcgaattaac aatacctctc tatacagaat gtccttgttg taaaacaaaa aatcccaaac
     5161 ctgttgggat tggtaccgag cgattagagg ccgcattaca agaaaatttt ccaacccagt
     5221 cgattgttcg cattgatcgc gacaccacac gtaaaaaagg tgaattaaaa aaagcaattg
     5281 atctgattca agagggcaaa gccgatatac tgattggtac acaaatgatt gcaaaagggc
     5341 atcattttcc gaatgtgact ttggttggga tcttagatat tgatcacgct ttatttagca
     5401 cggattttcg cagtatcgaa aaaatgggac agcttattac acaagtggca ggacgatcgg
     5461 ggcgcgcaga gcgaccagga cacgttattc tacaaacctg tcatccggaa catcctctac
     5521 tgaaacaatt
//
LOCUS       DAORVT010000028         5518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_9979_length_5518_cov_3.724144, whole genome shotgun sequence.
ACCESSION   DAORVT010000028 DAORVT010000000
VERSION     DAORVT010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5518
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9979_length_5518_cov_3.724144"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..296)
                     /locus_tag="PK389_00990"
     CDS             complement(66..296)
                     /locus_tag="PK389_00990"
                     /inference="COORDINATES: protein motif:HMM:NF015980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22321.1"
                     /translation="MGLLIWCITSIANAGLYVEITGGVAAGIPIAIVPFGVEGSYSDS
                     VEDLSKIIKSDLENSGQFKALSFQSIEEFPTH"
     gene            complement(326..1282)
                     /gene="tolA"
                     /locus_tag="PK389_00995"
     CDS             complement(326..1282)
                     /gene="tolA"
                     /locus_tag="PK389_00995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HQY22322.1"
                     /translation="MKRWALLHNGSIELSPAHYTPYFLASVLMHVLLLVFFIFSLEAS
                     YNDNQLPAKPGEIVQATIVDQTKVDEEVRRLKTAEKQDSQEQALREKQLTQKLEQLKH
                     AREQEQVKLDQLKKDMVIAKQQEQERLAEIKIFKEKEQKQLEDLKREKEAEKKKLAAL
                     DDQRQAEQDRAKQMRQEREKEEKKKSEAKRKATEAKQVAAAKAAKDAAIIAERAAVES
                     QSRITSEVQKVLGMWAERIKSNKREAFGLATDLYCKLAINVLPDGNVKVRLLQSSGNA
                     IYDDLSIKAVYKSEPFELPEDPMVREQIKSFELGLRNDEGSE"
     gene            complement(1290..1703)
                     /gene="tolR"
                     /locus_tag="PK389_01000"
     CDS             complement(1290..1703)
                     /gene="tolR"
                     /locus_tag="PK389_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017445950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HQY22323.1"
                     /translation="MRKHTKRRLVSEINIVPYVDVMLVLLVIFMVTAPLLTQGVQVHL
                     PQAKAEPLPQDQKPPIVVSVDASGHLFLDQSKESIQPGDLAIKVAASIQVDPTRSVLV
                     RGDKNVDYGKVMNAMVLLKRAGVSKVGLMTEHTEE"
     gene            complement(1696..2436)
                     /gene="tolQ"
                     /locus_tag="PK389_01005"
     CDS             complement(1696..2436)
                     /gene="tolQ"
                     /locus_tag="PK389_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007107171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HQY22324.1"
                     /translation="MTPDQSLIGLFLGASLLVKIVMLLLLAVSLVSWTLIFQRWKLFG
                     YALKTVDGFEDTFWSGIELTKLYQQFSAGKRGHHEVSGSESIFLAGFKEFVRLRKQTG
                     IQPAAVMEGSQRAMRVAMAREVERLEMHLPFLATVGSVSPYIGLFGTVWGIMNSFRAL
                     GSVQQATLSMVAPGISEALVATAMGLFAAIPAVIAYNRYSNRLQGLVNRYETFFEEFT
                     SILHRQAYTLPTSSLNSYGVENYTENDA"
     gene            complement(2464..5043)
                     /gene="gyrA"
                     /locus_tag="PK389_01010"
     CDS             complement(2464..5043)
                     /gene="gyrA"
                     /locus_tag="PK389_01010"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HQY22325.1"
                     /translation="MADLAREITPVSIENELKQSYLDYAMSIIVGRALPDVRDGLKPV
                     HRRVLYAMRELGNEWNKPYKKSARVVGDVIGKYHPHGESAVYDTIVRMAQPFSLRYML
                     IDGQGNFGSVDGDPPAAMRYTEVRMAKIAQSLLADLDKETVDFVPNYDESEMMPMVLP
                     AAFPNLLVNGSSGIAVGMATNIPPHNLTEVIDACLQLIENPDLSVDALMKIIPGPDFP
                     TAGIINGRAGIIEGYRTGRGRVHVRARISIETDEKSQKQSIIVTELPFQVNKARLIEK
                     IAELVKDKKIDGITALRDESDKDGMRMVIELRRGEAADITINNLYTQTQLQTVFGINM
                     VALVDGQPRILNLRDLLVEFLKHRRQVVSRRTAFELRKSRDRAHVLEGLGIALANIDP
                     VIQLIKASPTPTEAKAGLMGRMWAPGIVADMLQRAGADVCRPEDLQVEFGLQEKGYRL
                     SAIQAQAILDLRLHRLTGLEQDKILEEFKSIILNIQDLLDILSNQTRLMTVIRDELLQ
                     IKQEFGDKRRTEILSDHLDLSTEDLITEEDRVVTLSHGGYVKAQPLSSYQAQRRGGKG
                     KAATKVKEEDYVVRLLIASTHDTVLCFSSLGKVYWMKVYEIPVASRTSQGRPIVNLLP
                     LQPNERISALLPVRAYTEDKMVFMATANGTVKKVALTEFMRPRSSGIIALELDEGDTL
                     IGVDITDGARDVMLFADTGKAVRFSETDVRTMGRTARGVRGIRLQSGQQMISLIVLKP
                     EGAILIATQKGYGKRTEVEEFSAKGRGGQGVIAIQTSERNGHVVGAVQVFNNDELMLI
                     SDGGTLVRTRVEEISVVGRNTQGVRLINLGEGEKLVGIQRIDEVESLEVAIDAEDNSP
                     VEI"
     gene            5234..>5518
                     /locus_tag="PK389_01015"
     CDS             5234..>5518
                     /locus_tag="PK389_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011174781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HQY22326.1"
                     /translation="MTLSSLIKQRCQQALAQAFPAKVSEIDFAMLDITEATQPKFGHY
                     QFNSAMKLSKILGENPRQIAEKMVKALNALSDGSTRRSTPTFSKIEIAGPG"
BASE COUNT         1557 a         1402 c          967 g         1592 t
ORIGIN      
        1 ttaatacgac cgacaaccaa atcttcgata cctaatcctt gccaataagc atactgcact
       61 tcattttagt gtgttggaaa ttcttcaatc gattgaaaac tcaaagcctt aaattgccca
      121 ctgttttcca aatctgattt gatgattttg gagagatcct caacgctatc agaataagac
      181 ccttctacac caaacggcac aatagcaatg ggtatccctg cagcaacgcc acctgtaatt
      241 tctacataca atccggcatt tgcaatggat gtaatacacc agatcaaaag ccccatgaaa
      301 caaaaaaaac ccattttaat acgcattatt ccgaaccctc gtcatttctt aaacctaatt
      361 caaaagattt gatctgctct ctcaccattg gatcttctgg caactcaaac ggctctgatt
      421 tgtaaactgc tttaatagac aaatcatcat aaatggcgtt cccgctactt tgcaaaagac
      481 gtaccttaac gttaccatcc ggcaaaacat taatggccaa tttacaatac aaatctgtcg
      541 ctaaaccaaa ggcttcacgt ttattgcttt taattctttc tgcccacatt cccaaaactt
      601 tttggacttc gctggtaatg cgactctgac tttcaaccgc agcacgttca gcgataatag
      661 ccgcatcttt tgcagccttc gccgcggcaa cctgttttgc ctcggtggct ttacgtttag
      721 cttcactttt ctttttttct tccttctcac gctcttgtcg catttgtttc gcgcgatctt
      781 gttctgcttg acgttgatca tctaatgcag ccaatttttt cttttctgct tctttctctc
      841 gctttaagtc ttcgagttgt ttctgttctt tttctttaaa aattttgatt tcagctaaac
      901 gctcttgctc ttgctgcttt gcaatcacca tatctttttt taattggtcc aatttaacct
      961 gttcttgttc acgggcatgc ttcagctgtt ctagcttttg agtcaactgt ttttctctga
     1021 gagcttgttc ttggctatct tgtttttcag ctgttttgag tcttctaacc tcttcatcga
     1081 cttttgtttg atcaacaatt gtcgcttgta caatttcacc tggttttgca ggcaattgat
     1141 tgtcgttgta actcgcttcg agactaaaaa taaaaaaaac taataaaaga acatgcatca
     1201 aaacagaggc taaaaaatat ggcgtataat gtgctggaga caactctatc gacccattat
     1261 gcagcagcgc ccatcttttc ataacaaccc tattcctccg tatgctcggt cattaatcct
     1321 actttagaaa cacccgcacg ttttaacaac accatcgcat tcatcacttt gccataatca
     1381 acattcttat caccccgaac caaaacagaa cgagtgggat ctacttgaat gcttgccgct
     1441 actttaatcg ccaaatcccc cggctgtatc gattctttgg attggtctaa aaataaatga
     1501 cccgatgcat caaccgacac aacaatgggc ggtttttgat cttgtggaag tggctctgct
     1561 ttcgcttgtg gcaaatgcac ttgtacacct tgcgttaaga gtggggctgt caccataaaa
     1621 ataaccaaca gcaccaacat aacatccaca tacggaacaa tgttgatctc ggatacaagt
     1681 cttcgcttag tatgtttacg catcattttc cgtatagttt tcgacaccat atgaattcag
     1741 tgaagatgta ggaagcgtat aagcctgtct atgtaaaatg ctcgtaaatt cttcaaaaaa
     1801 agtctcgtaa cggttcacca acccttgcaa tcgattagaa tatcggttgt aggcaataac
     1861 cgcaggaata gcggcaaaca atcccatcgc tgtcgcaacc aatgcttccg atattcccgg
     1921 tgctaccata gaaagtgtag cctgttgcac actgcccagc gctctaaaag aattcataat
     1981 tccccacaca gtaccaaaca atccaatata gggactgact gatccaacgg ttgctaaaaa
     2041 aggcaagtgc atttctaaac gttcaacttc tctggccatc gccacccgca tcgcgcgctg
     2101 tgagccttcc atcacagcag ccggctgaat acctgtttgt tttctcaggc gcacaaattc
     2161 tttaaaaccc gccaaaaaaa tactttcgga tccgctcact tcatgatgtc cgcgtttacc
     2221 tgccgaaaat tgttgataaa gcttcgtcaa ttctatgcca gaccaaaagg tatcttcaaa
     2281 accatcgacg gttttaagcg catagccaaa taatttccat cgctgaaaaa taagcgtcca
     2341 agaaaccaaa gacactgcca ataatagcag catcacgatt tttaccaata aactggcgcc
     2401 taaaaacaaa cctattaacg actgatcagg tgtcattact ttatcccctg cttagcttta
     2461 caatcaaatt tctacgggtg aattgtcttc agcatcaatc gccacttcaa ggctttcgac
     2521 ctcgtctatg cgttgaatgc cgactaattt ttcaccttca cctaaattga tcaagcgtac
     2581 accctgcgta ttgcgaccta caactgaaat ctcttccaca cgggtacgca ccaaggttcc
     2641 gccatcactt atcagcatca gttcatcatt attaaagact tgaactgcgc caacaacatg
     2701 accattacgc tcgctcgtct ggatcgcaat aaccccttgt ccgccacgcc ctttcgcaga
     2761 aaactcttcc acttctgttc gttttccata ccctttttgg gttgcaatta aaatagcacc
     2821 ttcgggcttt aaaacaatta aagaaatcat ctgttgccca gattgcaatc gaataccgcg
     2881 cacgccgcgt gcagtacgcc ccatcgttct cacatctgtt tcggaaaaac gaacggcttt
     2941 accggtatcc gcaaacaaca tcacatctct agcaccatca gtaatatcca cgccaatcaa
     3001 ggtgtctccc tcatctaatt ctagcgcgat aatgccgctg gatcggggtc gcataaactc
     3061 ggttaacgca acctttttga ccgtaccatt ggctgttgcc ataaagacca ttttatcttc
     3121 tgtataggcg cgaaccggca ataaagcgct gatgcgctcg tttggttgta agggcaacaa
     3181 attaacaatg gggcgcccct gagaagtgcg gcttgccacg gggatttcat agaccttcat
     3241 ccaatacact ttcccaagac tagaaaagca taaaaccgta tcatgtgtac tcgcgattaa
     3301 taagcgcacc acataatctt cttctttcac tttggttgca gcttttccct taccaccacg
     3361 ccgttgcgct tgatagctac ttaaaggttg tgctttaacg tatccaccat gcgacaaggt
     3421 tacaactcta tcttcttctg taatgagatc ttctgtagag aggtcgagat gatcgcttaa
     3481 aatttcagtt cgacgcttat ccccaaattc ttgtttgatc tgtaaaagtt catcgcggat
     3541 cacagtcatt aatcgggttt gatttgacaa gatatccaat aaatcttgga tattaagaat
     3601 aatgctctta aattcttcta aaattttatc ctgttcaagg cccgttaaac gatgcaatct
     3661 taaatccaaa atcgcttgcg cttgtatagc cgataatcga tagccttttt cctgcaagcc
     3721 aaattcaacc tgaagatctt cagggcgaca cacatccgca cccgcacgtt gcaacatatc
     3781 tgcgacaata ccaggtgccc acattcgccc cattaaacct gcttttgctt cagtgggtgt
     3841 gggagacgct ttaatcaact gtatgaccgg atcaatatta gctaaagcga tccctaaccc
     3901 ttctaataca tgcgcacgat cacgtgattt tcgcaattca aatgccgtac gacgcgaaac
     3961 cacttgacgc cgatgtttta aaaattcaac caataagtct cgtaaattca aaatgcgcgg
     4021 ttgtccgtca accaacgcaa ccatattaat accaaacacg gtttgtaact gtgtttgggt
     4081 ataaagatta ttaatggtga tatcggctgc ttctccacga cggagctcaa tcaccatacg
     4141 catcccatct ttatcggatt catcgcgcaa cgcggtaatg ccatctatct ttttatcttt
     4201 cactaattct gcaatttttt caatcaatcg cgctttatta acttgaaaag gcaattcagt
     4261 cacaataatc gactgttttt gagatttctc atcggtctcg attgaaatgc gtgcccgaac
     4321 atgtacacgc ccacgtcccg tacgatagcc ttctataata cccgcccgcc cattaataat
     4381 ccccgcggtt ggaaaatccg gacccggaat aattttcatt aatgcatcga cacttaaatc
     4441 aggattttca atcaactgca aacacgcatc gatgacttca gtcagattat gcggaggaat
     4501 attcgtcgcc atacccactg caataccgga actaccattt accaataaat taggaaatgc
     4561 ggccggcaat accattggca tcatctcgga ctcgtcataa ttaggaacaa aatccaccgt
     4621 ttctttatcg agatccgcta ataaggattg tgcaatttta gccatacgga cttctgtata
     4681 acgcatggca gccggtggat cgccatcaac agagccaaag ttaccctgac catcgattaa
     4741 catatatcgc agcgaaaaag gctgcgccat tcgcacaatc gtgtcgtaaa cagcggactc
     4801 tccgtgcgga tgatacttac caatcacatc acccacaaca cgcgcggatt ttttataggg
     4861 tttgttccac tcattgccca attcacgcat ggcatataac acccgccgat gcacgggctt
     4921 taaaccatca cgaacgtcag gtaatgctcg cccaacgata atactcatgg cataatccag
     4981 gtatgactgt tttagctcat tttctatgct aacgggggta atttctctgg caagatctgc
     5041 catggaacaa ctcctaaata cttgttttac agggcgggat tgtaccacgt tcctcttaaa
     5101 aactcacctg ttcttgacta gaaaatttag aaatgatatg atatttttct aactttataa
     5161 tgaattatcc gtaggggagg accgccgtgt ccgcccttgt ttgattgata aggtattaag
     5221 ctaaggatta acaatgacgc tttcaagtct cataaaacaa cggtgccaac aggctttagc
     5281 ccaagctttt ccggcaaagg tctcggaaat tgactttgcc atgctcgaca ttaccgaagc
     5341 cactcaacca aaatttgggc actaccaatt caacagtgca atgaagttaa gcaaaatttt
     5401 gggcgaaaat ccgagacaaa tcgccgaaaa aatggttaaa gcacttaacg ctctgtctga
     5461 cgggtcgaca cggaggtcaa cccctacatt ttcaaaaatt gaaatcgcag gtcccggg
//
LOCUS       DAORVT010000029         5448 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_10185_length_5448_cov_3.536065, whole genome shotgun sequence.
ACCESSION   DAORVT010000029 DAORVT010000000
VERSION     DAORVT010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10185_length_5448_cov_3.536065"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(448..1479)
                     /locus_tag="PK389_01020"
     CDS             complement(448..1479)
                     /locus_tag="PK389_01020"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014978154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HQY22327.1"
                     /translation="MTVKNSSIKDSPIAVLGAGSWGTALAIALAKNGQVVHLWDRVQP
                     LLADISAIRQNARYLPDVPLPDNIVICNTLALALEQVKDILLVVPSHGFAEALQSVKP
                     FLTPAHRIAWGTNGLEPETGHFLNQVAERELGAGRPYAVISGPSFAKEVAIGLPTAVT
                     VASINQKFADELAVRFSQDSFSVYTTDDIIGVQLGGVVKNVLAVAVGISDGVQFGANA
                     RAALITRGLNEMMRLGEVLGARRETLMGLAGCGDVILTCTDNQSRNRRFGLALAEGLT
                     EAEALNQIGQVVEAVYNVGQLCGLAKREQIELPIVEQVFRIMKQGVSPREALKVLFKR
                     APERESLTN"
     gene            complement(1479..2024)
                     /gene="secB"
                     /locus_tag="PK389_01025"
     CDS             complement(1479..2024)
                     /gene="secB"
                     /locus_tag="PK389_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HQY22328.1"
                     /translation="MPKNAQPPVNKAANGQDLHDPKAAVSTPVFNIHSIYVKDLSFEA
                     PHSPQIFNTEWKPKIDFDLQMGSQILSEPESIYEVVLHVTVTIKLGEAAEEKPAFLID
                     IQQAGAFSVQNFTPEATQQLLATTCATVLFPYARETVSNLATRGGFPQLVLPPINFDA
                     MYEHHLKTRDTTAESSVAESH"
     gene            complement(2060..2311)
                     /gene="grxC"
                     /locus_tag="PK389_01030"
     CDS             complement(2060..2311)
                     /gene="grxC"
                     /locus_tag="PK389_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HQY22329.1"
                     /translation="MKKVIVYSTAICPYCIRAKELLESEGIPFEEIRVDLDVARFEEM
                     KRISGQRTVPQIFIGEQHVGGFSDLKALYERGELHDSLR"
     gene            complement(2340..2768)
                     /locus_tag="PK389_01035"
     CDS             complement(2340..2768)
                     /locus_tag="PK389_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQY22330.1"
                     /translation="MEEWVVFITNHWILASLLGVVLTAFIINEIIGSHFGSKQAVSTE
                     RAVQMMNHEEAVVFDVRTEAEFSAGHIVGAEQLAAVSLDKKMSHLQKYLKQPIIIATA
                     TGQDTAKVMTLLQAKGFLQIFVLSGGMQAWKSAGMPVIKS"
     gene            2940..4469
                     /gene="gpmI"
                     /locus_tag="PK389_01040"
     CDS             2940..4469
                     /gene="gpmI"
                     /locus_tag="PK389_01040"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HQY22331.1"
                     /translation="MTSHPIALIILDGFGYSASLKFNAIKSAKTPVWDRLLEECPHTT
                     LDASGLAVGLPNDQMGNSEVGHLTIGAGRVLMSDLMQVSTAIENGHFNQNPVLIAAIE
                     KAKQPGCTLHILGLLSPGGVHSHESHIHALIELAKEKGVSNIVMHAFLDGRDTPPKSA
                     TNSLDALQKICRIGSLSGRYYAMDRDKRWERTKTVYELLTENKAPFTFSSALEALEGA
                     YSRNETDEFVQPTVITPSAPILDNDVVIFMNFRSDRARQLSITLTDPHFSGFKRPVFP
                     KLSEFVTLTEYANTITAKIAFPPTPLNNTLGEYLQNNHCRQLRIAETEKYAHVTFFFN
                     GGHETSFEGEDRILVPSKKVATYDLAPEMSAFELTEKLVAAIHEKKYDVIICNYANAD
                     MVGHTGNFDATVKAIEALDICLGQVIEALKAEGVDAIITADHGNAEMMFDEVTHQPHT
                     AHTLARVPFLYVGRPARINHEHGVLSDIAPTIISLLGQSKPIEMTGSVIMTLDLLATK
                     Q"
     gene            4620..>5448
                     /locus_tag="PK389_01045"
     CDS             4620..>5448
                     /locus_tag="PK389_01045"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HQY22332.1"
                     /translation="MQSRPEKMMTLLKASLIGSALILMPVTVSAEASSKEEKLPIADL
                     QRFTKVIEYIKEYYVKPVSDEVLFENAMRGMLAGLDPHSSYFSMDEFSDLKASTSGKF
                     GGLGVEVMPEEGFIRVISPIDDTPAQRAGIQSGDLIIKLDDTAVKGLTAQEAIEKMRG
                     EKGSKIVLTILRQGENKPLVITVIRDTINVQSVRTKMLDDNFGYVRVSQFQSNSGDEL
                     TRSLQALKKNNGGKLKGIILDLRNNPGGVLDTAVQIADTFLDRDKLKQYDIYSKSIYI
                     "
BASE COUNT         1632 a         1272 c         1041 g         1503 t
ORIGIN      
        1 cctcagcgga ccaacagtat cgtttgttat aggagcaaga ctaggagcag taacttctca
       61 atttcaggtt gagcagtctc atgcacaaac aagcgtgact agcgcaacaa gtatgagaac
      121 cacgggcgca agctctagta ctggtacatc tacaaaccca caataagaga cagttctttt
      181 ttctgcaaaa aaaacccccc aataatgggg ggtttcctca tgcaaaagtt ctatcgtaga
      241 aataacttat tttccacaac gttccttttc cattttgtga catactgtct tcgtatggca
      301 ttttggtgca catttctttt ggcagccaca gccgaccaac cctaacatta ccaaacttac
      361 cataacaact ttagctacat ttttcatctt taacgactcc ccttcagttt taattaaacc
      421 tgtaagatta ggttagacaa ctttttacta atttgtcaaa ctttctcttt caggtgccct
      481 tttaaataac accttcaatg cttcccgtgg cgaaacccct tgcttcataa tacgaaacac
      541 ctgttccact ataggtagct caatctgctc ccgcttcgct aaaccacaaa gctgacccac
      601 attgtacaca gcttcgacga cttgaccaat ctgatttaac gcttcagctt ctgttaatcc
      661 ttctgctaac gctaatccaa aacgtcgatt acgtgattga ttatccgtgc aggttaaaat
      721 aacatctcca caccctgcaa gtcccatcaa tgtctctcgg cgtgcaccta aaacttcacc
      781 taagcgcatc atttcgttaa gaccacgcgt tattaaggcc gctcttgcgt tagcaccaaa
      841 ctgtacgcca tctgaaatgc cgactgcaac cgctaataca tttttgacca ccccacccaa
      901 ttgaacgcct attatgtcat ctgtcgtgta aacgctaaaa ctgtcttgac taaaacgtac
      961 agccagttca tcggcaaatt tttgatttat tgaagcgaca gttactgcgg taggtaatcc
     1021 aatagccact tcttttgcaa agcttggccc cgaaataacc gcgtagggtc gaccagcacc
     1081 cagttcacgc tctgctacct gatttaaaaa atgacctgtt tctggctcaa gcccattagt
     1141 cccccatgca atacgatgcg caggcgttaa aaagggttta acactttgta aagcttctgc
     1201 aaacccatga ctaggtacaa ccaacaaaat atcttttacc tgctctaaag ccaaagcaag
     1261 cgtattacag ataacaatat tatctggcaa tggcacgtcg ggcagatatc gggcattttg
     1321 ccggatggct gatatatctg ctaataacgg ctgaacacga tcccacaaat gcacaacctg
     1381 accatttttt gccaaggcaa tggcaagtgc agttccccag gaacctgcgc ctaataccgc
     1441 aatcggtgaa tctttaatcg aactattttt aaccgtcatt aatgactctc agcaacagat
     1501 gattctgccg tcgtgtcacg tgttttaaga tgatgctcgt acatcgcatc aaaattaatg
     1561 ggtggtaaca ccaattgtgg aaaaccaccg cgcgtcgcaa gatttgaaac agtttcgcgt
     1621 gcgtatggga acaagacggt tgcgcaagtc gtcgccaata attgctgcgt agcctctggt
     1681 gtaaaattct gaacactaaa cgctccagcc tgctgaatat caattaaaaa cgccggcttt
     1741 tcttctgcgg cttctcccag cttgatggtt accgtaacat gtaacactac ttcataaatc
     1801 gactcgggct cggacaaaat ctgagaaccc atttgcaaat caaagtcaat tttaggcttc
     1861 cattctgtgt taaagatctg aggactatga ggtgcttcga aagagagatc tttaacataa
     1921 atactatgga tattaaacac aggcgtactc actgctgctt ttggatcgtg taaatcttgc
     1981 ccattagctg ctttattaac aggtggttgt gcattttttg gcatattgat gcttcctctt
     2041 tatattttga aaaaatgaat taccgaagcg aatcgtgcaa ttcgcctcgt tcgtacaagg
     2101 cttttaaatc cgaaaaacca ccgacatgtt gttcgcctat aaaaatttgt ggcactgttc
     2161 gttgcccact gatccgtttc atttcttcga agcgagctac atccaaatca acccgaattt
     2221 cttcaaatgg aataccttcg ctttccaata attcttttgc acgaatgcaa tagggacaaa
     2281 ttgctgtact gtagacaatg acttttttca tgggttcatc ccttgtttct ccttactgtt
     2341 catgatttta tcacgggcat cccggccgat ttccatgctt gcatcccacc acttaaaaca
     2401 aagatctgaa ggaatccttt tgcttgtaat aacgtcatca cttttgcagt gtcctgtccg
     2461 gttgctgtcg cgatgatgat tggctgcttc aggtatttct gcaagtggct cattttttta
     2521 tcgagtgaga ccgctgctag ctgctctgcg ccaacaatat gtcctgctga aaattctgct
     2581 tccgttcgga catcgaacac aaccgcttct tcatgattca tcatttgcac agcacgctct
     2641 gttgaaacag cctgttttga accgaaatga ctaccaatga tttcattgat aataaatgcc
     2701 gtcagaacaa ctcccagcag ggaagccaaa atccaatgat tagttataaa gacaacccat
     2761 tcttccatat tcaaccaact cttcatttaa atttaataac cgtgatacac gcaccaaaag
     2821 gggagtatac tcgaaatagg cgtttatgac actaaatgat ataaagccct atatgaaaac
     2881 gatgcgtaac cccctagaat actcagaatg catttttgcc ccacggacag agagacgcaa
     2941 tgacatcaca ccccatcgca ttaattatct tagacggctt tggctacagc gcatcactca
     3001 aatttaatgc cataaaatca gcaaaaacac cggtttggga taggcttttg gaagagtgtc
     3061 ctcatacgac cctagacgct tcgggcctgg ccgtcggact accgaatgat caaatgggca
     3121 actccgaagt tgggcattta accataggtg ctggacgcgt actgatgtct gatctcatgc
     3181 aagtttcaac cgcaatagaa aatggtcatt ttaaccaaaa tccggtattg atagcagcga
     3241 tagaaaaagc aaaacaaccc ggttgcacat tacacatttt gggattatta tcgcccggcg
     3301 gcgtgcacag ccatgaatcg catatccatg cactcataga actcgctaaa gaaaaaggcg
     3361 tctcaaacat tgtgatgcat gcatttttgg atggtcgaga taccccgcct aaaagcgcaa
     3421 caaattcttt agatgcatta caaaaaatct gtcgtatcgg atctctcagt ggacgttact
     3481 atgcaatgga tagagataag cgctgggagc gcacaaaaac ggtttacgaa ctcctcaccg
     3541 aaaacaaagc cccctttacc tttagttctg ctctcgaagc cttagagggt gcttacagcc
     3601 gcaatgaaac cgatgagttt gtacaaccca ccgtgattac accatctgca cctattttag
     3661 ataacgatgt tgttattttt atgaatttta gaagcgaccg tgcaagacaa ttaagcatta
     3721 cgttaacaga ccctcatttt tccggcttta aacgccctgt ttttccaaag ctttctgaat
     3781 tcgttaccct tacagaatat gccaatacaa tcactgcaaa aattgctttt ccaccaacac
     3841 cgcttaataa tacgctgggc gaatatttgc aaaataacca ctgtcgacaa ctccgcatcg
     3901 cggaaaccga aaaatatgca catgttacct tcttttttaa tggcggacat gaaacgtctt
     3961 ttgaaggtga agatcggatt ttagtgccat ccaaaaaagt cgcgacttat gatcttgcac
     4021 ccgaaatgag tgcttttgaa ttaacagaaa aattggtcgc ggccattcat gaaaaaaaat
     4081 atgacgtgat catttgtaat tatgcaaatg cagacatggt cggccatacg ggcaattttg
     4141 atgccaccgt aaaggcgatt gaagcattgg acatttgctt agggcaagtt attgaagcct
     4201 taaaggcaga aggcgttgac gcgattatca ctgcggacca cggaaatgca gaaatgatgt
     4261 ttgatgaggt cactcaccaa ccccatacag cccacaccct ggcgcgcgta cctttcctgt
     4321 atgttggccg tcctgccaga attaaccatg aacacggcgt cttatccgat atcgctccca
     4381 cgattatcag cttgttagga caatcaaaac ctatagagat gacaggaagc gtgataatga
     4441 cgctcgactt gctagcgaca aaacagtaag atcattttat tattaggatt cgaagacagc
     4501 agcttctgag aaagcggatt atactttcta aagtttgatt aaaggccaga aaggatagat
     4561 aatctattcg acaaacttat tcaacaaacg caccctgatc ccaaatggag aaattggcta
     4621 tgcaatccag accagaaaaa atgatgacac ttctaaaagc aagcctgatc ggatctgctt
     4681 tgatcctaat gccagtaact gtctctgcag aggcatcctc caaagaagaa aaactgccta
     4741 tcgctgatct ccaacgattt accaaagtca tcgaatatat taaagaatat tacgttaagc
     4801 ccgtctctga tgaagtctta tttgaaaatg ccatgcgtgg catgttggca gggcttgatc
     4861 cccattccag ctattttagc atggacgaat tttccgattt aaaagccagc accagcggta
     4921 aatttggtgg tttaggtgtt gaagttatgc cagaagaagg atttatccgc gtcattagtc
     4981 cgattgatga tacacccgcg caacgtgcag ggatccaatc aggtgatctg attattaaat
     5041 tggatgacac ggcagttaaa ggattaacgg ctcaagaagc gattgaaaaa atgcgtggcg
     5101 aaaaaggcag taaaattgtt ttaacaattc ttcgacaagg cgaaaataaa cctcttgtaa
     5161 taaccgttat tcgagatacc atcaacgtac aaagtgtacg caccaaaatg ttagatgata
     5221 attttggata tgtacgtgtt tcacaatttc aaagtaattc tggtgatgag ttaacgcgtt
     5281 cactgcaagc actcaaaaag aataatggcg gaaaattaaa aggcattata ctggatctcc
     5341 gtaataatcc aggcggcgta ttagacaccg ctgtacagat cgcggatact tttctcgatc
     5401 gcgataaatt aaaacaatat gatatatatt cgaaatcaat atatattt
//
LOCUS       DAORVT010000030         5412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_10309_length_5412_cov_3.342356, whole genome shotgun sequence.
ACCESSION   DAORVT010000030 DAORVT010000000
VERSION     DAORVT010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5412
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10309_length_5412_cov_3.342356"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /gene="truB"
                     /locus_tag="PK389_01050"
     CDS             complement(<1..587)
                     /gene="truB"
                     /locus_tag="PK389_01050"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010598459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HQY22333.1"
                     /translation="MFRNVNRREVQGILLLDKPKGISSNAALQIVKRLFKAKKAGHTG
                     SLDPVASGLLPICFGEATKFSQFLLEADKHYQTTAVLGIRTDTGDIEGNVVSERPVPD
                     LNSVDIETILDRFRGRISQIPSMFSAIKHQGQPLYKLARQGIVIEREARELTIKELKV
                     LELKNNTMRLEIRATKGTYVRTLVEDIGEALGCGA"
     gene            complement(577..927)
                     /gene="rbfA"
                     /locus_tag="PK389_01055"
     CDS             complement(577..927)
                     /gene="rbfA"
                     /locus_tag="PK389_01055"
                     /inference="COORDINATES: protein motif:HMM:NF014130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HQY22334.1"
                     /translation="MPKNFSRSQRVADAVHQCLARVIRGEFKDPRVGMITVLNVEMTP
                     DLKHAKIYVSVLEEDKVSETIKVLNKAAGFFRAQIANNIQLRVVPNPRFIFDDSVIRG
                     NRIAQILESVASVS"
     gene            complement(927..3371)
                     /gene="infB"
                     /locus_tag="PK389_01060"
     CDS             complement(927..3371)
                     /gene="infB"
                     /locus_tag="PK389_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HQY22335.1"
                     /translation="MGPGETVDQEADKPKRITLKRQSSEVITVQRSGRKGTVSVVHKR
                     RHVYVKKDASAETEEVSLQQQETLNIASESAAPPEELSEKIVSETPEALVAESDVASG
                     ISADISADASLASPPELVNAQSVSEGHGVPKKGRSAKELLSDEEKKRTRSKSKIRSEP
                     EEWHKGNIVKVLEEIVTEEVDEIEGEPVVERPVWRKTKLNKNAAARGSKRELRGIVEE
                     IQKKHSFEKPVGPIVRAVLIPETVTVAELAQKMSAKAGDLIKTLMKLGTIATINQPID
                     QDTAVIVVEEMGHTAKRVNDITVEESLQVTESRELMPRAAIVTVMGHVDHGKTSLLDY
                     IRTTKVAAKEAGGITQHIGAYHVRTKRGDITFLDTPGHAAFTAMRARGVQCTDIVVLV
                     VAADDGVMPQTIEAIQHAKAASVPIVVAINKIDKKDADLDRIQNELVTHGLVPETWGG
                     DVMIIPVSAKEGTGVDALLEALGLQAEMLELKAPVAGKATGVVLEARLDKGRGPIASL
                     LVQSGTLKQGDILLAGSEYGRIRAITNELGEQITTIGPAMPIEVLGLSGTPQAGDIFM
                     VVVDERKAKEVATFRQIKQREARMLRQQATKLEGFFDRMKQGESKILNIVLKSDVHGS
                     AEALMEALEGLSTDEVKIKIVASGVGGINESDVSLAMASKAVLIGFNVRADVSARRLM
                     EKEGVDVYYSGIIYDVVDNIKRAINGLLGPQFKENILGLASVRSVFRSAKLGAVAGCM
                     VIEGVVKRGCPIRVLRNNIVIYQGELESLRRFKEDVSEVRNGTECGIGVKNYNDVKEG
                     DNIEVFETVVVARTTV"
     gene            complement(3387..4906)
                     /gene="nusA"
                     /locus_tag="PK389_01065"
                     /pseudo
     CDS             complement(3387..4906)
                     /gene="nusA"
                     /locus_tag="PK389_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006034741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
     gene            complement(4940..5398)
                     /gene="rimP"
                     /locus_tag="PK389_01070"
     CDS             complement(4940..5398)
                     /gene="rimP"
                     /locus_tag="PK389_01070"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HQY22336.1"
                     /translation="MRRHAALENLIAPIVGSLGLTWVGLQYFPQGKRSVLRLFIDKPG
                     GLSVEDCGRVSRQVDTVLSVEKPITGEYTLEVSSPGLDRLLFSLEQCHEQIGKIVSVQ
                     LVAPKAGARNFKGRLERVEGSLISLLTESGDLSFSFDEMSEMRLVPVWSV"
BASE COUNT         1446 a         1437 c          950 g         1579 t
ORIGIN      
        1 tgtgcaccac agcccaatgc ctcaccgata tcttctacaa gcgttcttac gtacgtccct
       61 ttagtcgcac gtatctctaa acgcatcgta ttatttttga gttctaaaac tttaagctcc
      121 ttaatcgtga gttcgcgtgc ttctctctca atcacgattc cttgacgcgc cagtttatat
      181 aaaggttgac cttgatgttt aatggccgaa aacatcgaag gaatctgcga aatacggcct
      241 ctaaaacgat ccaaaatggt ttcaatatcg acgctattta aatctggaac aggacgttcg
      301 ctaacaacat taccttctat atcccccgta tcggttcgta tacccaatac tgcggtcgtt
      361 tggtaatgtt tatctgcttc taatagaaac tgcgaaaatt ttgtggcttc tccgaaacaa
      421 atcggtaaaa gaccactggc aacgggatct aaactacccg tatgacccgc ttttttcgct
      481 ttaaacaagc gttttactat ttgtaatgca gcgttagagg agatcccttt gggtttatct
      541 aacaacaaaa taccctgaac ttctcgccga tttacattac gaaacactgg caacagactc
      601 caaaatttgc gcaatgcgat ttccgcgaat caccgaatca tcaaaaataa aacgaggatt
      661 tggaacgacg cgaagttgaa tgttattggc gatttgtgct ctaaaaaatc ccgcggcttt
      721 atttaaaacc ttgattgttt cggagacttt atcttcttcc aaaaccgaca cataaatctt
      781 ggcatgcttt agatcgggcg tcatttcgac atttaatacg gtaatcatcc ccacgcgcgg
      841 atctttaaat tctccgcgaa tcacacgcgc caagcattga tgcaccgcat ctgcaacacg
      901 ttgagatcgg ctaaaatttt taggcattaa acagtcgtcc ttgcaacaac gactgtttca
      961 aagacttcaa tattatcgcc ttctttaaca tcattataat ttttaacccc aataccacat
     1021 tccgtgccat ttcgaacctc agaaacatcc tctttaaaac ggcgtaaaga ctcaagctca
     1081 ccttgataaa tcacaatgtt atttcttaaa acacggatcg gacacccacg tttaaccaca
     1141 ccctcaataa ccatacaacc cgcaacagca cccagttttg cagaacgaaa aacgcttcga
     1201 acggaagcca gtcccagaat attttcttta aactgtggac ctagcaaccc attaattgca
     1261 cgtttaatat tatcaacgac atcataaata ataccgctat aatatacatc gacaccttct
     1321 ttttccatca accttcgcgc agaaacatcg gcacgcacgt taaatccgat aagcactgct
     1381 ttggatgcca tggctaaaga gacgtccgat tcattaatac caccaacacc cgaagcaacg
     1441 attttaattt taacttcatc cgtggacaac ccttctaagg cttccattaa agcttcggct
     1501 gaaccatgaa catcggattt taatacgata tttaagattt tcgattcgcc ttgtttcatg
     1561 cgatcgaaaa agccttctag tttagtggct tgttgtctta acatacgcgc ttcacgctgt
     1621 ttaatttgtc taaacgtcgc gacttctttc gctttacgtt catcgacaac aaccataaat
     1681 atatcgcccg cctgtggcgt acccgacaaa cctaatacct caataggcat tgcaggacca
     1741 atagtggtaa tttgctcgcc taattcattt gtgatcgcac gaattcgacc atattctgat
     1801 cctgctagca atatatcgcc ttgttttaaa gtaccgcttt gtacgagcag agaggcaata
     1861 ggacctcgac ccttatctaa acgcgcttct aagacaacac ccgttgcctt gcctgcaacc
     1921 ggcgctttta attccagcat ttcggcttgt aaacccagag cttctaataa cgcatcaacg
     1981 ccagttcctt ctttagcaga aaccggaata atcatcacat caccgcccca ggtttctgga
     2041 accaagccat gcgtcactaa ttcattttga atacgatcga gatcggcatc ttttttatca
     2101 attttattaa tcgcaacaac gataggaaca cttgctgctt ttgcatgttg aattgcttca
     2161 atcgtttgtg gcatcacacc atcgtctgca gcaacaacga gcaccacgat atcggtacat
     2221 tgcacgcctc gcgcacgcat tgcggtaaag gcagcatggc ctggggtatc taaaaaggta
     2281 atgtcaccgc gtttagttct cacatgatac gcgccaatat gctgcgtaat accgcctgct
     2341 tctttagccg ctactttagt ggtacgaata taatctaaca gcgatgtttt gccgtgatca
     2401 acatgtccca tcaccgttac aatagccgca cgcggcatta attcccgaga ctctgtaacc
     2461 tgcaaagatt cttcaaccgt aatatcattc acgcgcttgg cagtatgtcc catttcttca
     2521 accacaataa cagccgtatc ttgatcaata ggttgattaa ttgtggcaat cgttcctaat
     2581 ttcattaatg ttttgatgag atcgcctgcc tttgcagaca ttttttgcgc caattccgca
     2641 accgtaacag tttcgggaat taacacggcg cgaacgatag gacctaccgg tttttcgaag
     2701 ctatgtttct tttgaatttc ttcaacaatg cccctcaact ctcgcttact gcctctggca
     2761 gctgcatttt tattcaattt agttttacgc cagacaggac gttcgacgac aggttctccc
     2821 tctatttcgt ccacttcttc tgttacgatc tcttctaata ctttgacgat atttccctta
     2881 tgccactctt cgggttcact gcgaatttta gatttagaac gcgtacgttt tttctcttca
     2941 tcactcaata actcttttgc actgcggcct tttttaggca ccccatgccc ttcgcttaca
     3001 ctctgcgcgt taacaagctc tggaggtgaa gcaagagagg cgtcagcaga tatgtcagct
     3061 gaaatgcctg atgcaacgtc gctttcagcc acaagcgctt caggcgtctc agaaactatt
     3121 ttttccgata attcttctgg gggtgcagca ctctccgagg cgatattcaa agtctcttgt
     3181 tgttgtaaag aaacttcttc tgtttccgca cttgcatctt ttttaacata gacatgacgt
     3241 cttttatgta caacactaac ggtacccttg cgacccgatc gctgtacggt aattacttcc
     3301 gaactctgac gttttaaagt aattctttta ggtttgtcag cctcttgatc aactgtttcg
     3361 cctggtccca tggacctacc ctccaatcat acaaaccaat gtgcgcgtgc tgccataatg
     3421 agctcagcag ctttagattt atccaaatct ggcacatcgg ccagatcgtc taccgcgagt
     3481 tctgctaaat catcacgtgt cattatccca cggtttgcta aaacaaaagc taattccttg
     3541 tccataccgg gaagtgaagc aaatcttcgg cgggtgtttt ttcaattaac ttttcttccg
     3601 ccggtaccgc ttgcgttaac aacgcttgct ttgcacgagt acgcaactcg ttaacaatct
     3661 cttcatcaaa gccttccacc cctaataatt cttgtatagg cacatacgca atctcttcga
     3721 gactcgtaaa tccttcttca attaatactt ctgcaacatc attttcaaca tgtagatgtt
     3781 ccgtaaaaac attgactaac cgctcttgat ccgcggttgt tctctctttg gcttctttgt
     3841 cggtaatcac atttaatgtc caaccggtaa gctggctcgc caatcgcaca ttttgtccac
     3901 tgcgtccaat tgcttgcgat aaatgatcct ctgtaacagc cacatccatc gattgcgcgt
     3961 cttcgtctac aacaatagaa acaatttcag cgggtgccat tgcattcata acaaactgcg
     4021 caggactgtc gtcccataac acaatatcaa cgcgctcacc gcccaattca tttgagacag
     4081 cctgcacacg tgcaccacgc atcccgacac aagcaccaac aggatcaata cgcccatcat
     4141 tggttttaac ggcaatcttc gcacgaatac ccggatcacg tgcagcgctt ttaatttcga
     4201 taataccttc tccaacttcc ggtacttcga gcttgaagag ctctatcagc atctcattac
     4261 aggtacgact taagattaat tgcgggccac gtgcttctgt cacaacatcg actaaataaa
     4321 cgcgcaatct atcacctggc cgaacggctt ctcgaggtaa catatgctct ttggataaaa
     4381 aggcttctac gttggcacct aatttcgcat tgccacccaa ctccaaaata atggcttctc
     4441 ggctcgtctt tttaacaaca ccgatgacaa tctcaccaat ttttttacga aattcatctg
     4501 caacattttc gtgttctgct tcacgcactt ttttcaaaat aacttgttta gcggcttgcg
     4561 ctgcgatacg tccaaattcc acggattcaa taaggaattc gatgacatcg cccgctttag
     4621 ctgtcttaga atgttttaag gcttctgaaa gcggcatttc tcgcaagggg aattctatct
     4681 cgggctgttc cgtgttaggt tcgtcgacga ctgtccaaac acgaaaggtg tcatattgac
     4741 ccgttttacg attgatgcta acgcgtacat ccgagtctac gccttccttt tttttggtcg
     4801 cagaaaccaa tgcagcctca atcgcctgaa atattatctc ttcagaaact cccttttcat
     4861 tagaaagggt ttctacaacc aattttagcg gaatctcttt gctcatataa aagcctcaca
     4921 ctgtctatcg tctgccctat cacacggacc acactggcac caatcgcatt tcactcatct
     4981 catcaaagga aaagcttaag tctcccgact ccgttagaag agaaatcaaa gatccttcca
     5041 cacgttctaa cctgccctta aaatttcgcg cgcctgcctt tggtgcgacc aactgaacgg
     5101 atactatttt gccaatctgc tcatgacact gttccagtga aaacaacaat cgatccaagc
     5161 ctggtgaaga cacttccaag gtgtactcgc ctgtaatcgg tttctctaca ctgagaaccg
     5221 tatccacttg ccgactaacg cgtccgcaat cttccacgct aagcccacct ggcttgtcta
     5281 taaacaatcg caataccgat cgctttcctt gcggaaagta ctgaagacct acccaggtaa
     5341 gccccaaact acccacaatg ggtgcaatca ggttttctaa ggccgcgtgt cgtctcataa
     5401 tcccacttca ac
//
LOCUS       DAORVT010000031         5295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_10687_length_5295_cov_3.656298, whole genome shotgun sequence.
ACCESSION   DAORVT010000031 DAORVT010000000
VERSION     DAORVT010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10687_length_5295_cov_3.656298"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PK389_01075"
     CDS             complement(<1..298)
                     /locus_tag="PK389_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002208527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF188 domain-containing protein"
                     /protein_id="HQY22337.1"
                     /translation="MRVWVDADACPNAIKEILFRAAMRTKTVVTLVSNNFLSTPVSPF
                     IQKMQVPMGFDVADKKIVDSLEKNDLVITADIILADLVVDKGGTALNPRGKLYTP"
     gene            479..1195
                     /locus_tag="PK389_01080"
     CDS             479..1195
                     /locus_tag="PK389_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22338.1"
                     /translation="MADNFFMNEETKDRRTYKDLIQNRTQYLPLSGIVGVVTEFLSYG
                     IKLPVIGRLLMRSFTVVCCAIGRLRRKSDYNGLYDLLVHCLSHNTFRKHEEYRWWYLM
                     RFAVAVAQERQINWLIRDIRLEDNLVLLGSLGPRPLKGYDVAYAFVGYSLWMFERGDV
                     LGAINLIQIAEQADETWGYPEYLHGWYGLFTRDVDSVTHFTRAVHIDWSFLQRMKHDK
                     TCREHPDVLHAVQKRTLVAR"
     gene            1192..2760
                     /gene="gshA"
                     /locus_tag="PK389_01085"
     CDS             1192..2760
                     /gene="gshA"
                     /locus_tag="PK389_01085"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HQY22339.1"
                     /translation="MNSFFLKRLQMLESPDWLQSLSGIKRGFEKECLRVNKQGLISTK
                     PHPKTLGSSLTHPMITTDFSEALLEFITPPTENLNAPYDILNALHLYTNQNLKDELLW
                     SASMPCTLPKETEIPIAEYGSSNLGRLKHIYRRGLAFRYGRIMQTISGIHYNFSLSDN
                     FWQKYYAHSGSSLKKQLFISEQYLGLIRNSLRFGWLLPLLFGASPAVSTSFFAEHQPD
                     LEVHGSDTYVGSFATSLRLSDLGYHNREEMKDKISYNTFSTFLETMRKAVHTSVPQYR
                     RIGVFKDGEYNQLSDCVFQIENEHYALVRPKRVSGREERMMSAILRDGIEYIEVRALD
                     INPFVPIGIEQDMVYLLDAFLIYCLLSDSPLLTDAEDQQIEYNHSQVVERGRQPKLSL
                     LDGHGKPRELVDWAMELLTSIEAVAVLLDKAYGSQAFTNACQIAKKRVLEPDTLPSAQ
                     VLKEMKAEGRSYFDFASHWSVKHQQYFRNQQMLPSELKAYNTLVQNSFVAQKELESTD
                     TLSFDKYLSLYLAT"
     gene            2773..3189
                     /locus_tag="PK389_01090"
     CDS             2773..3189
                     /locus_tag="PK389_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22340.1"
                     /translation="MKVVNIKKTALLLMFLLILDSVFATEQPSLASIADSLVVGTDAV
                     TRLLHFVSIVVGCFLFIMAFSLFRANRLNPKFVPLERPIIYVFLGIILVALPFYGKIF
                     MPTGSTIDLKIEEERAMGVQSHDIDAPLEWGNDYDH"
     gene            complement(3212..4651)
                     /locus_tag="PK389_01095"
     CDS             complement(3212..4651)
                     /locus_tag="PK389_01095"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HQY22341.1"
                     /translation="MQKLLIVIVIYFSNTVFAIETNFPDMGESSETILEAKNQRVIGN
                     MIMQKIHGSDFLVADPIVNEYLHGIAGHFTSQPHLRHINLDIFGVNTPEFNAFAFFGG
                     HIAVHSGLILGVNNESELVAVLAHETAHISQRHLARIMDASRQMMPLTAAEILAAIAV
                     GALGAPEAGIHLATAAMAGHMQSMINFTREHEKEADRVGIQMLSQTEFDPNAMASVFQ
                     CMKKNSQYQTRPPEYLLTHPVFDSRIADAQNRAATFKQKQATDSLSFHLVRARLEIAM
                     EESTHKKIHRLKEKLDSGRYQNKIAAEYAYALSLLKSPKQTNTGLTRLQNLNTQNPHQ
                     WMLELGLAEAEHITGQLDAALNRTKRLMEAYPSNYAIVLQHITLLLQNKQPEVAVKLL
                     VAQRQMHFNDPTLHQMLARAYSLSHQPTDLHRSQAEWHFARGEFKDAFKQLDLALEYA
                     QNKKSVLSKIHQRKEAMQATIEEQEDLKL"
     gene            complement(4695..>5295)
                     /locus_tag="PK389_01100"
     CDS             complement(4695..>5295)
                     /locus_tag="PK389_01100"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY22342.1"
                     /translation="RTFYAKHIILATPPHVSAKLLLSNSESTDLCKELITNLLKFHYQ
                     PISTVYLRYANEINLIKPMIGFINSTLHWMFDRRFAGQPDVLSIVISSSGAHSLLSHA
                     ELVAKIQQELESTCTNLQVFPTLHTAPLDYRVVTEKRAAFSCDIGINDYRPRTSTPLV
                     NLWLAGDYIEPCYPATLEGAIRSGIKAAQLILSKDALFA"
BASE COUNT         1506 a          949 c         1182 g         1658 t
ORIGIN      
        1 taggcgtgta aagcttaccg cgcggattta aagcggtgcc gcctttatct accactaaat
       61 ctgctaagat aatatcggcg gtaataacga gatcgttttt ctcaagactg tctacaattt
      121 ttttatctgc cacatcaaaa cccatgggta cttgcatctt ttgaataaaa ggagatacag
      181 gagtacttag aaaattgttt gatactaaag taaccacagt ttttgtgcgc atcgctgctc
      241 gaaataaaat ttccttaatg gcatttggac aagcatctgc atcaacccat acacgcacta
      301 gcatcccctt cagcttgctg tgcccaaaaa tacctcgctg aaatagagaa agttactttt
      361 gcgcaatgcc tttaacggag caaaatatca ggattccgcc aaaaaatcaa taaaaaaagt
      421 caaaaaaaat agcctgttca tatgttccgt tttctaagcc gttcatgggt cgatgcctat
      481 ggccgataac ttttttatga atgaagaaac aaaagaccgt agaacataca aagaccttat
      541 tcagaatcga actcaatatt tgccattgtc gggtattgta ggcgttgtga ctgaattttt
      601 aagttatggc attaagcttc ctgttatagg tcggctactg atgcgctcat ttacggttgt
      661 ttgctgtgcg atcgggcgtc tgcgcagaaa aagtgattat aatgggctgt atgacttact
      721 tgtacattgt ttatctcata acacctttag aaagcacgag gagtaccgtt ggtggtattt
      781 aatgcgcttt gcagttgccg ttgctcaaga gcggcaaatt aattggttaa tacgggatat
      841 tagattagaa gacaatcttg ttttattagg cagtcttggc ccaaggcctt taaaagggta
      901 tgatgtagcc tatgcctttg tgggatactc gctttggatg ttcgagcgcg gtgatgtttt
      961 aggggctatc aacctgattc aaattgcaga acaagcagac gagacctggg gctaccctga
     1021 atatttacat ggttggtatg gtttatttac acgggatgtt gattcagtaa cgcattttac
     1081 gcgcgccgtg catattgatt ggagcttttt acaaagaatg aaacacgata aaacctgtag
     1141 agaacatccg gatgtgttgc atgcagtaca aaaaagaacg cttgttgcaa gatgaattct
     1201 ttttttttaa aacgattaca aatgttagaa tctcctgatt ggcttcagag tttatcgggg
     1261 ataaaacgtg ggtttgaaaa agagtgttta agggtaaata agcagggact tattagtacc
     1321 aaaccacatc ctaaaacatt gggatctagc ttaactcacc ctatgattac caccgatttt
     1381 tcggaggcct tattagaatt tattacacca cccactgaaa atttgaatgc gccttacgac
     1441 atactcaacg ctttacatct atataccaat cagaacttaa aagatgaatt attatggtct
     1501 gcaagtatgc cgtgtacgct ccctaaagaa acagaaattc cgattgctga atatggttct
     1561 tccaatcttg gacggcttaa gcatatttac agacgtggtt tagcgtttcg ttatggtcga
     1621 attatgcaaa cgatttcagg tattcattat aatttttcgt tgtcggataa tttttggcag
     1681 aagtattatg cacattcagg ctcatccttg aaaaaacagt tatttatatc agaacaatat
     1741 cttggcctga ttcgaaacag tttgcgattt ggttggttgc tacccttatt gtttggtgca
     1801 tcacctgcgg tatcgacttc tttttttgca gagcatcaac cggatttaga agtgcatggt
     1861 tcggatacct atgtgggatc ttttgctacc tcattacggt tgagtgattt agggtatcat
     1921 aatcgagagg aaatgaagga taaaatttcg tataatacgt tttcaacatt tttggaaaca
     1981 atgcgaaagg cggtacatac atccgtacca caatatcgtc gaataggtgt ttttaaagac
     2041 ggtgaatata atcaactcag cgattgcgtg tttcaaattg aaaatgaaca ttatgcatta
     2101 gtgcgcccca aacgagtttc tggtcgcgaa gagcgaatga tgtctgcgat tttacgcgat
     2161 gggatagaat atatcgaagt gcgcgctttg gatattaatc cgtttgtacc gataggtata
     2221 gaacaggaca tggtttatct attagatgct tttttaatct attgtttatt aagtgatagt
     2281 ccattattaa cagatgcaga agaccagcaa attgagtaca atcatagcca ggttgtagaa
     2341 cgtggtcgtc aaccaaagtt gagtcttctt gacggacacg gcaagcctcg tgaattagtg
     2401 gattgggcta tggaattatt gacatcaata gaagctgtag cagtattatt ggacaaggct
     2461 tatggatctc aagcctttac aaatgcatgt cagattgcaa aaaaacgggt tttagagccc
     2521 gatactttgc cgtctgcgca ggtattaaaa gagatgaaag cagaggggag atcttatttt
     2581 gattttgcga gccattggtc tgtaaagcac cagcagtatt ttcgaaatca acaaatgctt
     2641 ccttcagagc ttaaggctta caacacattg gttcagaatt cttttgtggc tcaaaaggaa
     2701 ctagagagta cagacacttt atcgtttgat aaatatttaa gtttatatct tgcaacgtag
     2761 aggatttatg gcatgaaagt tgtaaatata aaaaaaacag cattattact gatgttttta
     2821 ttaatattag acagtgtttt tgcaacagaa cagccaagcc tagcgagtat tgcagattca
     2881 ctggttgtcg gaactgatgc ggtaacacgg ttgctgcact ttgttagcat tgttgttggg
     2941 tgttttttat ttatcatggc attttcttta tttagagcaa atcggttgaa tccaaaattc
     3001 gtccccttag aaaggccgat tatttatgta tttttaggca taattttagt tgctttgccg
     3061 ttttatggca aaatatttat gccaacggga agtacgattg acttaaaaat agaagaagag
     3121 cgtgctatgg gtgtgcaatc gcacgatatc gatgcacctt tagaatgggg aaatgactac
     3181 gatcattgag gaagctattt cgcgcacctt cctataattt taaatcttcc tgttcttcta
     3241 tggttgcttg catcgcttct ttgcgctgat gaattttact taaaacagat tttttatttt
     3301 gcgcatattc caaagccaaa tccaattgtt taaaagcgtc tttaaactcg cctcttgcaa
     3361 aatgccattc ggcttgtgag cgatgcagat cggttggttg gtgagataag ctataagcac
     3421 gcgctaacat ttgatgcaac gtcggatcgt taaagtgcat ttggcgttgc gcaacaagta
     3481 atttgacagc aacttcaggt tgtttatttt gcaataggag tgttatgtgt tgtaggacaa
     3541 ttgcataatt agagggataa gcttccatta aacgttttgt gcgattgaga gcagcgtcga
     3601 gttggcccgt gatgtgttct gcttctgcaa ggccgagctc aagcatccat tgatgtggat
     3661 tttgagtatt caggttttgt aagcgcgtta agcctgtgtt tgtttgtttg gggcttttca
     3721 gtaaagaaag tgcataagca tattctgcgg ctattttgtt ttgataccgg ccgctgtcta
     3781 gcttttcttt gaggcgatgt atttttttgt gcgtactttc ttccatagcg atttcaagac
     3841 gtgcacgaac caaatggaaa gagaggctat cggttgcttg tttctgttta aatgtggcag
     3901 cgcggttttg ggcatcggcg atacgtgaat caaacacagg gtgcgtgagt aagtattcgg
     3961 gcggtcttgt ttgatattgg ctattttttt tcatgcattg aaaaacactc gccatggcat
     4021 tggggtcaaa ttctgtttga gagagcattt gtatacccac acggtcggct tctttttcat
     4081 gttctcgcgt aaaattaatc atgctctgca tgtggcccgc catggcagca gttgctaaat
     4141 gtatgcccgc ttcgggcgcg cccaaggcgc cgactgcgat agccgctaga atttctgcag
     4201 cagttaatgg catcatctga cgactggcat ccatgatgcg tgctaaatgc ctttgtgaga
     4261 tatgggctgt ttcatgcgcg agcacagcga ctaattcgct ttcgttattt acacctaaaa
     4321 tcaaaccact gtgaacagca atgtgtccac caaaaaatgc aaaagcatta aattccggtg
     4381 tgttgacgcc gaaaatatcg agattgatgt gacgaagatg gggttgagac gtaaagtgtc
     4441 cagctatgcc atgcaaatat tcgttaacga tgggatctgc aaccaaaaaa tcgctcccat
     4501 ggattttttg cataatcata ttgccaatga cccgctggtt tttagcttct agaatggttt
     4561 cggaggattc tcccatatcc ggaaaattgg tttcgattgc aaaaaccgtg ttagaaaaat
     4621 aaattacaat aacaataagg agtttttgca ataacttgag caatagagaa gctctgtcat
     4681 ctaaagaaaa aacattaagc aaatagcgca tctttagata gtatcagttg tgctgctttt
     4741 atgccactcc ggattgcgcc ttcgagtgtt gcagggtagc aaggttcgat atagtcacct
     4801 gcaagccaaa ggttaacaag aggggtggaa gttcggggtc ggtaatcatt gatcccaata
     4861 tcacatgaga aagcagcacg tttttctgtt acaacacgat aatcgagcgg tgctgtatga
     4921 agtgttggga atacttgaag attggtgcat gtgctttcga gctcttgttg aatttttgcg
     4981 actaattcag catgagacag taaactatga gcaccgctac tgctgataac aatgcttaat
     5041 acatcgggtt gtcccgcaaa gcgtcggtca aacatccaat gtagggttga atttataaag
     5101 cctatcatag gctttataag attgatctcg ttagcgtagc gtaaatagac agtgctaatt
     5161 ggctggtaat gaaattttaa taaattggtt attaattctt tacagagatc cgtagattct
     5221 gaattcgaca ggagtaattt tgctgaaaca tgcggtggtg ttgctaatat aatgtgcttt
     5281 gcataaaatg tgcgt
//
LOCUS       DAORVT010000032         5288 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_10704_length_5288_cov_3.750812, whole genome shotgun sequence.
ACCESSION   DAORVT010000032 DAORVT010000000
VERSION     DAORVT010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10704_length_5288_cov_3.750812"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PK389_01105"
     CDS             <1..334
                     /locus_tag="PK389_01105"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:NF020720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HQY22343.1"
                     /translation="ELRRLGSGPYTAEQMLSFEILEHILEQEGIEKLESYLLPVDTSV
                     AFMPDLRVSEATSYYLRQGQPVQIPYAPTSGWVRFSSQNGRFLGVGEILEDGRVAPRR
                     LTQEMVSL"
     gene            390..659
                     /gene="rpsO"
                     /locus_tag="PK389_01110"
     CDS             390..659
                     /gene="rpsO"
                     /locus_tag="PK389_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011253110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HQY22344.1"
                     /translation="MSTTSNAKSAIIEKFRRATADTGSPEVQIALLSTRITELTEHLK
                     IHKKDFHSRRGLIAMVNRRRKLMAYLKSENLPRYTKIVQDLSLRG"
     gene            752..2842
                     /gene="pnp"
                     /locus_tag="PK389_01115"
     CDS             752..2842
                     /gene="pnp"
                     /locus_tag="PK389_01115"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007145765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HQY22345.1"
                     /translation="MTVIKKTIPFGDHQLTIETGVIARQATASVVVSLDDTTVLVTVV
                     GLREPKVGQSGFPLRVDYQERAYAAGKIPGGFFKREGRPSERETLISRLIDRPIRPLF
                     PKGFWNEVQIVATVISLNPEIDSDIPAMIGASAALALSGLPFNGPMGAARVAYVDGQY
                     LLNPTQTQLGSSELELVVAGTSHSVLMVESEAHELTESVMLGAVMFGHKQFQPVIELI
                     KSLVTDAGKPAWDWKPAVENIELANEIKAASVTALTEAYQIQDKQARYQSLKTIKNTL
                     IENHSVGSPNSGTPELKIEISKILENLDRSIVRGRILANQPRIDGRNTKTVRPITIQP
                     SWLPRAHGSVVFTRGETQAIVVVTLGAGRDAQLIDSPLGEYKDAFMLHYNFPPYSVGE
                     VGMVGSPKRREIGHGMLAKRAMRAVLPKDSDFPYVLRVVSEITESNGSSSMATVCGAS
                     IALMDAGVPIKSAVAGIAMGLIKENDQFQVITDILGDEDHLGDMDFKVAGTDKGITAL
                     QMDIKIDGITEEIMRVALEQAKEGRLHILGVMNQALAEPRVEMSSFAPQIISFQINPE
                     KIRDVIGKGGATIRSLTEDMDATVDIDDNGTIHIASSDKAAAAEVRRRIDLITAEAEV
                     GKIYEGKVVRITEFGAFVNFLPGKDGLVHISQITDRRIETVDEVLQEGQTVKVKVLEI
                     DRQGRVRLSMKEVE"
     gene            complement(2864..3553)
                     /locus_tag="PK389_01120"
     CDS             complement(2864..3553)
                     /locus_tag="PK389_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HQY22346.1"
                     /translation="MYFSKFITEQHRSARIGWLRAAVLGANDGIISTASLIVGITAAH
                     TPHHSILIAGVASLIAGSMSMAAGEYVSVSSQLDTEQADIERERIELENNLEYEHEEL
                     ANIYISRGLQSSLAKQVAAELMAHDALGAHARDELGITETLKARPLQAAITSAITFSV
                     GAALPLLMVIVAPFTQLIPAVSGISLLSLALLGALASFTGGSNMLTGTMRVTLWGALA
                     LATTTGIGMLF"
     gene            complement(3562..3861)
                     /locus_tag="PK389_01125"
     CDS             complement(3562..3861)
                     /locus_tag="PK389_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22347.1"
                     /translation="MSIEMDGARAILSTYRDVHDTPQTNVSVQTVRSKLQQKNRNLQS
                     ELSTLKEKYLILQHELINRDKKLETLKNQLIDKTLHMTRLEEDFENAIEQLGTKP"
     gene            complement(4055..>5288)
                     /locus_tag="PK389_01130"
     CDS             complement(4055..>5288)
                     /locus_tag="PK389_01130"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HQY22348.1"
                     /translation="IETFQNVCSPAVQEDLKVFKKLLTNPETLAPKSTLSESNKADTL
                     SFIKHYILSNLHLLDSRTAILPNTFMVQKSIMEGEHLKSVVHSSALRNVIMSAHYFDS
                     LEKKFSEEGISKERIEPMVKNAKIYTHELHKAVLEGNISRLLKCVAVHGVDINFPNEQ
                     GMTPLHIAVQEGLTETVKILLTVPLIKLTAINNNGWTPLHIAARLGFSDIADALLTMP
                     TIDPNAVNSDGWSALHWAAWHGFTETVTVLLAAPGINLNLKDRNDTSPLHLAARNGDP
                     DVISVLLSAQGIDLNILDNEQHTPLHLAAIYNHEAAIKVLLKDAHIEVNLADMDGLTP
                     LHWAARNGHLEILNDMLVHPDIIVDCMDNNFMIPMEWATRNGHISITKILKPYTTKHT
                     AIYSFFNKLLMILRLKPV"
BASE COUNT         1527 a         1019 c         1175 g         1567 t
ORIGIN      
        1 ggaattaagg cgattaggat caggtcctta tacggcggaa cagatgcttt cttttgaaat
       61 cttggaacat attttagaac aagaaggtat tgaaaagtta gaaagttatc tattacctgt
      121 cgatacttct gttgctttta tgcctgatct acgcgtttct gaggcgacaa gttattatct
      181 acgacaaggt caacctgtcc agatccccta tgctccaaca tctggttggg tacgctttag
      241 ctctcagaat ggtcgatttt taggcgttgg ggagatattg gaagacgggc gagtggcgcc
      301 tcgacgccta actcaagaaa tggtttcttt ataggggaag atcaggtaaa ctgaacccca
      361 tttgaagctc aatattagga gaaaattgaa tgtcaacaac cagcaacgca aagagcgcga
      421 ttattgaaaa atttcgacgt gcaacagcag ataccggatc ccctgaggtt cagattgctt
      481 tgttaagcac ccgtattaca gaattaacgg aacatcttaa aattcataaa aaagactttc
      541 attctcgccg tgggcttatt gcaatggtta atcggcgtcg taagttaatg gcttacttga
      601 agagcgaaaa tctgccgcgc tatactaaaa ttgttcaaga tttaagtctt cgaggttaat
      661 tactccacat gcctcctcaa ctccctctgt gaagggggca tttgctaagg tggagtgagg
      721 gctttattca atattctcat aaggattaaa cgtgaccgtc attaaaaaaa ccattccatt
      781 tggtgatcat caattaacaa tagagaccgg tgtcattgcg cgtcaagcaa ccgcttcggt
      841 agtggtgagt ctagacgaca caaccgtgtt ggtgacggtt gttggtttgc gggaacctaa
      901 ggtagggcaa agtggttttc cgcttcgcgt tgattatcag gaacgcgcgt atgcggctgg
      961 aaaaattcct ggcggttttt ttaaacgtga aggacgaccc agcgagcgcg aaactttgat
     1021 ttcccgctta atcgatcgac ccattcgtcc gctatttcca aaaggttttt ggaacgaagt
     1081 tcaaattgtg gcgaccgtta tttccttaaa tccagaaatt gactccgata ttcccgctat
     1141 gattggcgct tctgcagcat tagcgctttc tggtttaccc tttaatggcc ctatgggtgc
     1201 tgctcgggtt gcctatgtag acggtcaata tcttttaaat cccacccaaa cccaactggg
     1261 atcttctgaa ttggaattag ttgtggcggg cacttcacat tccgtgttga tggtcgaatc
     1321 tgaagcacat gaattaacag agtctgttat gttaggcgct gtgatgtttg gtcataaaca
     1381 atttcagccg gtcattgaat taattaaatc gttggtaaca gatgctggta agcccgcatg
     1441 ggattggaaa cctgctgttg aaaatattga attagccaat gaaattaaag cagcttctgt
     1501 aacagcttta acagaagcgt atcaaattca agataaacaa gcgcgatacc aaagtttaaa
     1561 gaccattaaa aatacactga tcgaaaatca ttcagtcgga tcgcccaatt ctggaacgcc
     1621 cgaattaaaa atagaaattt caaagatcct tgaaaatctt gatcgttcta ttgttcgagg
     1681 ccgtatttta gcaaaccagc cacgaatcga tggtcgaaat actaaaacgg ttcgtccaat
     1741 taccattcaa ccgagctggt taccgcgtgc acatggttct gttgtattta cacgaggtga
     1801 gacacaagcc attgtggttg ttaccttggg tgcagggcgt gatgcgcaat taatagacag
     1861 ccctttaggt gaatacaaag atgcatttat gctgcactat aattttccgc catattctgt
     1921 aggggaagta ggtatggtgg gtagtcctaa acgtcgtgaa attggacacg gtatgttggc
     1981 taagcgtgca atgcgtgccg tgttgcctaa agattcagat tttccgtacg tgttaagagt
     2041 tgtttcagaa attacagaat cgaatggttc gagttctatg gccactgttt gcggcgcaag
     2101 tattgcactg atggatgcag gtgtgcccat taaatcggca gttgcaggta ttgcgatggg
     2161 cttgattaaa gaaaatgatc aattccaagt catcaccgat attctaggag atgaagatca
     2221 cttgggcgat atggatttta aagttgcagg aaccgacaaa ggcattaccg ctttacaaat
     2281 ggatatcaaa attgatggga ttaccgaaga aattatgcgt gtggctttgg agcaagctaa
     2341 agaaggtcgc ttacatattt taggtgtgat gaatcaagca cttgctgaac cgcgcgttga
     2401 aatgtcttcg tttgcgcctc aaattattag ttttcaaatt aaccctgaaa aaatccgaga
     2461 tgttattggt aaagggggcg caaccattcg ttctttaacg gaagatatgg atgcaacagt
     2521 tgatattgat gataatggca cgattcatat tgcctcttcg gataaagcgg cagcagctga
     2581 agtgcgacgc agaattgatt tgattacagc tgaggcagaa gttggaaaaa tttacgaggg
     2641 taaagtagtt cggattaccg aatttggtgc ttttgtcaat tttctaccag gtaaagatgg
     2701 gttagtgcat atttctcaga ttaccgatcg ccgtattgag acggtggatg aagtcctgca
     2761 agaaggacaa acagtcaaag ttaaggttct tgaaatcgat cgccaaggtc gggtgcgttt
     2821 aagtatgaaa gaagttgaat gacgtgagag gctagcgggt tcttcaaaaa agcattccaa
     2881 taccggttgt tgttgctaat gccagtgcac cccataaagt cacgcgcatt gtccccgtta
     2941 gcatattgga accacctgta aaagaggcta aagcacctag taaagccaga cttaataaag
     3001 atatgccaga aacggctggg atcagctgag taaagggtgc gacaataacc atcaacaatg
     3061 gcaatgccgc tccgacagaa aatgtaatag cagaagtgat ggcggcttga agcgggcgtg
     3121 ccttgagggt ttctgtaata cccaattcat cgcgcgcatg tgcacctaag gcatcatgag
     3181 ccattaattc cgcagcaact tgtttggcaa gactcgattg aagtccacgt gaaatataaa
     3241 tattcgccaa ctcttcgtgt tcatattcta aattgttttc cagttctatg cgttcgcgtt
     3301 caatatcggc ttgttcggta tctaactgcg aactaaccga aacatattct ccagcagcca
     3361 ttgacataga acctgcgatt aggcttgcta cgccagcaat cagtatgctg tgatgaggtg
     3421 tatgcgctgc ggtgataccg acaataaggc ttgctgtaga aataatacca tcgtttgcgc
     3481 ccaacacagc agcccttaac cagccaattc gagcactacg atgctgttct gttatgaatt
     3541 ttgaaaaata cattctttat cttaaggctt ggttcctaat tgttcaatcg cattttcaaa
     3601 atcttcttct agccttgtca tatgtaatgt tttatcgatc agttggtttt ttaaggtttc
     3661 taatttttta tctctattaa taagttcgtg ctgtaaaatg agatattttt ctttgagcgt
     3721 gctgagttcc gattgaagat ttctattttt ttgctgtaat tttgagcgaa cagtttgtac
     3781 ggaaacatta gtctgaggtg tatcatgcac atcacgatag gttgataaaa ttgctctagc
     3841 gccatccatt tcaatagaca tgacatacct cgttgttggt tgaaaaagtt acctctaaaa
     3901 tcagaacgta aaccgataat gaaattaagc ttagtacatg ctcttaaaaa aaatcaagcc
     3961 aataaaataa ataattttag taaacttaat ctaggtcaag ggtgctttgt cgcgctttgc
     4021 aagcaatgac agaggaattt tagaacctta gactctagac aggttttagc cttaggatca
     4081 ttaacaattt attgaagaaa gaatatatag cagtatgttt tgtcgtataa ggctttagaa
     4141 ttttggtaat ggaaatgtgt ccattccgtg ttgcccattc catcggaatc ataaaattat
     4201 tatccataca atccacgata atatcaggat gcactagcat gtcattgagt atttcgagat
     4261 gaccatttcg agccgcccaa tgaaggggcg ttaagccatc catatctgcc agattaactt
     4321 caatatgtgc atccttcagc aaaactttaa tggcagcctc gtggttataa atagcggcta
     4381 gatgaagagg agtatgttgt tcgttatcaa gaatatttag gtcaatgcct tgtgcagaca
     4441 gcaaaacaga aataacatcg gggtctccat tgcgtgcggc tagatgtaaa ggtgatgtgt
     4501 catttcgatc ttttaagtta agatttatgc ccggtgctgc taataagacc gtcacggttt
     4561 cggtaaaccc atgccaagca gcccaatgta aggcactcca accatcgcta ttcacagcgt
     4621 ttggatcaat agtcggcatg gtgagaaggg catcggcaat gtccgaaaac ccgagacgcg
     4681 ccgctatatg tagcggcgtc caaccattgt tgtttatagc ggttagcttt atcagtggaa
     4741 cagtcaacaa tattttgact gtttccgtta atccctcttg aacagcaata tgtaaaggcg
     4801 tcatgccttg ttcgtttgga aaattaatat cgacgccatg tacggcaaca cattttaaga
     4861 gcctggaaat attgccttct aataccgcct tgtgtaattc atgtgtgtaa atttttgcat
     4921 ttttaaccat aggttctata cgttcttttg aaataccttc ttcgctaaat tttttttcaa
     4981 gactatcgaa ataatgtgca ctcatgatga cattgcgtag cgcactcgaa tgcacaacgg
     5041 actttaaatg ttcaccttcc ataatcgatt tttgaaccat aaacgtattg ggcagtatag
     5101 cggttctcga atctaacaaa tgcagattac tcaaaatata atgtttaata aaactcaggg
     5161 tgtctgcttt attgctttct gacaaagtgg atttgggtgc cagtgtttct gggttggtta
     5221 acaatttttt aaatactttc aaatcttctt gtacagcagg agaacagacg ttttgaaaag
     5281 tttcaata
//
LOCUS       DAORVT010000033         5283 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_10721_length_5283_cov_3.781752, whole genome shotgun sequence.
ACCESSION   DAORVT010000033 DAORVT010000000
VERSION     DAORVT010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5283
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10721_length_5283_cov_3.781752"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="PK389_01135"
     CDS             <1..694
                     /locus_tag="PK389_01135"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HQY22349.1"
                     /translation="RFYQASTSELYGKVQETPQTETTPFYPRSPYAAAKLYAYWITIN
                     YREAYGMHASNGILFNHESPARGETFVTRKITKAVAAIAKGSQEPLVLGNIDAKRDWG
                     HARDYAEGMWRILQQETPDDYVLATGETYSVREFVELAFKEVGITIEWRGKGLEELGI
                     NQENGKIVVKISPEFFRPTEVDLLLGDATKAKHKLGWTPTVTFRELVKEMVTADLENE
                     RGTHENAIFTRQ"
     gene            672..1607
                     /locus_tag="PK389_01140"
     CDS             672..1607
                     /locus_tag="PK389_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HQY22350.1"
                     /translation="MLFSLDNKRVYVAGHNGMVGSALVRRLANENCEILTVDRKQVDL
                     RDQTAITAWLAQNKPDVIFLAAATVGGIHANRSKPAQFLYNNLMIASNIIHAAKECGV
                     QKLLFLGSSCIYPRNASQPITEEALLTGSLEPTNEWYAIAKIAGLKLTESYRQEYECD
                     FISCMPTNLYGPLDNYDLENSHVLPALLRKIHEAKHQNQKTVTLWGTGKPRREFLFVD
                     DLADACVYLIKHYSDSKTINIGTGTDLMISELAETIAEVLGFKCEFTYDTSMPDGTPQ
                     KLLDISRLKALNWVAKTNLQTGIKFTYEEYLRRHL"
     gene            1604..3040
                     /locus_tag="PK389_01145"
     CDS             1604..3040
                     /locus_tag="PK389_01145"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HQY22351.1"
                     /translation="MITPIILSGGGGTRLWPLSRTHFPKQYWALVNEETLLQETALRL
                     ASTTQFHQPLVICNQEHRFLVAEQLREINLIPSHIVLETESHNTAPAIAIACLLQADP
                     DELLLVLPADHVIQNPEAFHAAVDSARAVAKQGKIVTFGIQPQRAETRYGYIERGTPL
                     NIHNNKRVFEVSRFIEKPDLDTAEMFLTDKNYYWNSGIFLFKTSTMLAELERLHPAIL
                     AGCKTAIANAKQDLDFLRLDSKAIVSDASLSIDYAVMEHTPHAVVIPVDMEWSDVGSW
                     DALWAISDKDAEGNAVIGDVITHDVQNSYIRADNQLVSVVGLDNVVVVATDDAILVAS
                     KDRVYQIKAVIEQLKLNNRKELNMHSRVYRPWGYYQHIDCGERFQVKRIMVKPDEKTS
                     TQIHYHRSEHWVVVEGIAKVTRGNETLLIHENESVYLPMGMKHRIENPGKIPLHLIEV
                     QSGSYLGEDDIVRIEDAYGRAKLEPNAV"
     gene            complement(3027..3656)
                     /locus_tag="PK389_01150"
     CDS             complement(3027..3656)
                     /locus_tag="PK389_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011713932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HQY22352.1"
                     /translation="MKFSSEQISKCVEKARDQWLDAIELKKQCLTGSYLNTLVSMAEV
                     AVRAMAKGGKLMLCGNGGSAADAQHLAAELLVRLRSHTNRDAIPALSLVLDTSTITAC
                     ANDYGYECLFERMVNGLGRPEDVLLGLTTSGRSVNVIRALKAARERGIATLGFLGGDG
                     GQTIQECDLSIIVPSSDPNRVQEIHITLGHILMDLIEGLMIEKEHVKRH"
     gene            complement(3676..5124)
                     /locus_tag="PK389_01155"
     CDS             complement(3676..5124)
                     /locus_tag="PK389_01155"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HQY22353.1"
                     /translation="MTVQKTKRILVVELNAAEKHFLDKYVAEGKLPVFKKLLQAGKVV
                     KTRVGARIEQLTPWTIWPSIYTGIKPDKHGIIGFGQAFDALQGYYIWDVLNAKGITTG
                     VFGNRISALVPQNPLSLFYVPGVFSAAQSCFPESLSFIQKFFVFIKKNNIHRSVGLAI
                     QLFFQLVMGAIKGVPFSIIKAILLQMRLELVKGMAYQKDRAILRAKCQMDIFKTLYNQ
                     YKPQFSTIHLENIASAQHLYWRAAEPDGFSDELGDLDSFYFENIEMRKSHEILFKDMI
                     LDNFRLVDGFLGQLLPLLDEQTVLVIITGLGQKKRDPVHQIHKPEIRFYNLKHLLNRI
                     DVLNCEILTQANPYITLNFRDTADASLGAFKLSNLNVLGEYPLFKLEQREHQLFLEEN
                     LSPTMWALGADVWVENKSNEMIFPLFNYVKISQLRDQYTADYSSEGWVLFYGNTTAFP
                     GNMDEITSPFDVMEIYPLLLSYFNDKIQGLDQ"
BASE COUNT         1621 a         1155 c         1005 g         1502 t
ORIGIN      
        1 ccgtttttat caagcatcta cttctgagct ttatggaaaa gtgcaagaaa cgcctcaaac
       61 agaaaccacg cccttctatc ctcgcagccc ttatgcggct gccaagctct acgcgtattg
      121 gataaccatc aattaccgcg aagcttatgg catgcatgct tcgaatggca ttttatttaa
      181 ccatgaaagc cctgccagag gcgaaacctt tgtaacgcgt aaaattacaa aagcagtcgc
      241 cgctatcgcg aaaggaagcc aagaacccct cgtgctcggt aatatagatg ccaaacgaga
      301 ttggggacat gcacgggatt atgccgaggg catgtggcgt attttgcagc aagaaacacc
      361 cgatgattat gtgcttgcaa caggcgaaac ctattcagtt cgtgaatttg tagaattggc
      421 ttttaaagaa gtcggcatta cgattgaatg gcgaggaaaa gggctagaag agctgggaat
      481 taatcaagaa aatggtaaaa tagtcgttaa aatcagcccc gaattcttta gaccgacaga
      541 agttgatctc ttattaggcg atgcaaccaa agcaaaacac aagctcggct ggacaccaac
      601 tgtcactttt agagaactgg taaaagaaat ggtcaccgct gatttagaaa atgagcgagg
      661 aacacatgag aatgctattt tcactcgaca ataaacgtgt ctacgttgcg ggccataacg
      721 gtatggtggg tagcgcctta gtacggcgtt tagcaaacga aaattgtgag atactaacgg
      781 tagaccgtaa acaagttgat ctgcgtgatc aaactgcaat tactgcatgg ctcgctcaaa
      841 acaagcctga tgttattttt ttagccgccg cgactgtggg tggcattcat gccaatcgtt
      901 cgaaacccgc tcaattttta tataacaatc tcatgatcgc cagcaatatt attcatgcgg
      961 ctaaagaatg tggtgtacaa aaattattgt ttttgggttc ctcttgcatt tatcccagaa
     1021 atgcttccca gccaataaca gaggaagcac tattaaccgg cagcttagaa ccaaccaatg
     1081 aatggtacgc gattgcaaaa attgcgggac ttaaattaac tgaatcatac agacaagaat
     1141 atgaatgtga tttcatcagt tgtatgccga ccaatttata tggcccttta gacaattatg
     1201 accttgaaaa tagccatgtt ttgcctgctt tattgcgaaa aattcatgag gcaaagcacc
     1261 aaaaccaaaa gactgtcacc ctgtggggga caggcaaacc caggcgagag tttctttttg
     1321 tggatgatct cgcagatgct tgtgtttatt tgataaaaca ctattcagat tctaaaacca
     1381 ttaatattgg aacgggtaca gatcttatga tttctgagct tgccgaaacg attgctgaag
     1441 ttctcggctt taaatgcgaa tttacctacg acacttcaat gcccgatggt acgcctcaaa
     1501 aattgctcga tattagccgt cttaaagctc tcaattgggt cgcgaaaaca aacttacaaa
     1561 ccggcattaa attcacctat gaagagtacc tcaggagaca cttatgatta cacccattat
     1621 tttatccggt ggtggtggca cacgtctttg gccactgtca cgcacacatt tccccaaaca
     1681 atactgggca ttggttaatg aggaaacgct cttacaagaa acagcattgc ggctcgcttc
     1741 gacaacgcaa tttcatcaac ctttggtaat ttgtaatcaa gaacatcgtt tcttagtagc
     1801 tgaacaatta cgagaaatca atcttatacc ttctcatatt gtattagaaa cagaatccca
     1861 taatacagcc cccgctattg ctatagcttg tcttttgcaa gcagatccgg atgaattatt
     1921 attggtattg cctgcagatc atgttattca aaaccccgaa gcttttcatg ccgcggttga
     1981 ctccgcacgc gctgttgcaa aacaaggcaa aattgttact tttggcattc aacctcagcg
     2041 cgcagaaacc cgctatggtt acattgaacg cggtacacct ttaaatatac ataataacaa
     2101 gcgtgttttt gaagtctctc gctttatcga aaaacccgat ctcgataccg cggagatgtt
     2161 tttaaccgat aaaaattatt attggaacag tggcattttt ctttttaaaa cgtctaccat
     2221 gctagcagaa ctagaacgac tacaccctgc aatactggca ggctgtaaaa cagcgatcgc
     2281 caacgctaag caggatctcg attttttaag gctagactct aaagccatcg tttctgatgc
     2341 cagtttatct attgactatg ccgtgatgga acatacccca catgctgttg ttatacctgt
     2401 ggatatggag tggtctgatg tgggatcttg ggatgcacta tgggcaattt cagacaaaga
     2461 tgcagaaggg aatgcggtca ttggagacgt gattacccat gatgttcaga actcctatat
     2521 ccgagccgat aaccaactgg tatcggttgt cggactcgat aatgttgttg ttgtcgccac
     2581 tgatgatgct attttagttg caagcaagga tcgcgtttat caaataaaag cggttattga
     2641 acaattaaaa ctgaacaacc gaaaagaatt aaacatgcat agccgcgtgt atagaccttg
     2701 gggatattac caacacattg attgtggtga gcgttttcaa gtaaaacgca ttatggtaaa
     2761 acccgatgaa aaaacatcca cccaaataca ctaccatcgc tctgagcatt gggttgtggt
     2821 tgaaggaatc gcaaaggtca cgcgaggcaa tgaaacgctt ttgatccacg aaaatgaatc
     2881 ggtttactta ccaatgggca tgaaacaccg tattgagaat cctggtaaaa ttccactgca
     2941 tttgattgaa gtgcaatccg gatcttattt aggtgaagac gatattgtcc gcattgaaga
     3001 tgcttatggg cgggcaaagc ttgaacctaa tgccgtttaa catgctcttt ttcaatcatc
     3061 aacccttcaa tgagatccat aaggatatgc cccaaggtaa tgtgtatttc ttgaacgcga
     3121 ttcggatcgc tagaaggcac aataatcgaa agatcgcatt cttgaatggt ttgaccacca
     3181 tctccaccca aaaaacccag tgtcgcaata ccacgctcac gtgctgcttt taaggcacgg
     3241 atcacattaa cagatcgacc cgacgttgtt aggcccaata aaacatcttc tggtctccca
     3301 agcccattga ccattctttc aaaaagacat tcatagccat aatcgttcgc acaggcagta
     3361 atggttgaag tatcgagcac caaagacagg gctggaatag catctcgatt tgtatgtgat
     3421 cttaatctca ccaataattc ggctgccaaa tgttgtgcat ctgcagccga accgccattg
     3481 ccacataaca tcaattttcc gcccttagcc attgcacgaa cagcaacttc agccatcgaa
     3541 acgagtgtat ttaaatatga acctgttaaa cactgttttt taagctcaat cgcatccagc
     3601 cactgatcgc gagccttttc tacacacttt gatatttgtt cagaagagaa tttcattaga
     3661 atgccccttt tttgtctatt ggtccaaacc ttgaatttta tcattaaaat aacttaataa
     3721 caatggataa atctccatca cgtcaaaagg ggaagttatt tcgtccatat tccctgggaa
     3781 tgcagtcgtg ttaccgtaaa acaaaaccca cccttcactg ctgtaatccg cggtatattg
     3841 atctcttaac tgactgattt taacataatt aaacaatgga aaaatcatct catttgattt
     3901 attttcaacc caaacatctg cacctaaagc ccacatagtc ggtgataaat tttcttctaa
     3961 aaacaattgg tgctcacgtt gttctagttt aaataatgga tattctccca gcacattcag
     4021 atttgacagt ttaaacgccc ccaaacttgc atccgcagta tccctaaagt tcagcgtgat
     4081 atagggattt gcctgagtta aaatctcaca attcaaaaca tcaattctat ttaataagtg
     4141 tttaaggtta taaaatcgaa tttctggttt atgaatttga tgcacaggat ctcgtttttt
     4201 ttgacccagt cccgttataa taaccaagac ggtttgctcg tctaatagtg gcaacaattg
     4261 gcctaaaaac ccatctacca atctaaaatt atctaaaatc atatctttaa ataaaatctc
     4321 atgtgatttt ctcatctcaa tgttttcaaa ataaaagctg tccaagtccc cgagctcatc
     4381 actaaaccca tcaggctctg ctgctcgcca gtacaagtgc tgtgcagagg caatattttc
     4441 caaatgtatt gtcgaaaatt gaggtttata ttgattatac aatgtcttaa aaatatccat
     4501 ctgacattta gctcttaata ttgctctgtc tttttggtac gccattccct ttacgagctc
     4561 caaacgcatt tgcaataaaa tggcttttat aatcgaaaat ggcacccctt taatagcacc
     4621 catgactaac tgaaaaaaca actgaatcgc taatccaaca gaacggtgga tgttattttt
     4681 ttttataaaa acaaaaaatt tctgaataaa gctgagtgat tctggaaaac acgactgagc
     4741 ggctgaaaaa acaccaggca cataaaaaag gcttaagggg ttttgaggaa caagggcact
     4801 tatccgattt ccaaatacac cggtcgtaat ccctttcgcg tttaagacat cccaaatata
     4861 atacccctgc aatgcatcaa acgcttgtcc aaatccaatg ataccgtgtt tatcgggctt
     4921 gatcccggta taaatagaag gccaaatcgt ccaaggtgtt aattgttcaa tgcgcgctcc
     4981 cacacgcgtc tttacaactt tcccggcttg cagcaatttt ttaaaaacgg gcaatttccc
     5041 ttcagccaca tatttatcta aaaaatgctt ctctgccgcg tttaattcaa cgactaatat
     5101 tctcttagtt ttttgtacag tcatcatttt tctcaaattt tgtcatttaa ctagaatttg
     5161 tcagaaacac gcaaaatcgt cacttgatat agactaccgg ctgtattttg ggtatgctct
     5221 tgtcagttaa tactattaat agcagagttc aaaataaatg accattctcg taaccggtgc
     5281 cgc
//
LOCUS       DAORVT010000034         5138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_11269_length_5138_cov_3.536691, whole genome shotgun sequence.
ACCESSION   DAORVT010000034 DAORVT010000000
VERSION     DAORVT010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11269_length_5138_cov_3.536691"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..372
                     /gene="rpsJ"
                     /locus_tag="PK389_01160"
     CDS             61..372
                     /gene="rpsJ"
                     /locus_tag="PK389_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HQY22354.1"
                     /translation="MASQVIKIRLKSYDHRLIDKSAKEIVDTAKRTGAQVRGPVPLPT
                     RHERFTVLVSPHVDKNARDQYEIPEHKRYVEIVEPTEKTVDALMKLDLAAGVDVQIAV
                     S"
     gene            383..1057
                     /gene="rplC"
                     /locus_tag="PK389_01165"
     CDS             383..1057
                     /gene="rplC"
                     /locus_tag="PK389_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HQY22355.1"
                     /translation="MIGVVATKCGMTRIFDAESGASIPVTVAIVEPNRIVQMKTLEND
                     GYIAIQVTRGEVKPSRITKAEAGHFAKANVVAGKDLMEFRAEAEDCKDLTVGSEIGAD
                     FFKVGQKIDVAGLTRGRGFAGCIVRHNFSSQRASHGNSLSHNTPGSIGQNQTPGRVFK
                     GKKMAGQYGNTQRTVQNLEVVRIDLERHVILIKGGVPGAPGGKLIIYPAVKHNIQSNA
                     QSTVAA"
     gene            1068..1688
                     /gene="rplD"
                     /locus_tag="PK389_01170"
     CDS             1068..1688
                     /gene="rplD"
                     /locus_tag="PK389_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HQY22356.1"
                     /translation="MEIAFAVPSHEAPKKGLEVSAITFGRDFNEALVHQVVTAYLAGA
                     RSGTRAQKTRAEVRGGGAKPWRQKGTGRARAGTIRSPLWRGGGRIFAAKPQDHSQKIN
                     KKMYRGAMQAILSELVRQDRLLVVDAIEISQPKTRDLLAKLKALGLKEVLIVTGQWDP
                     NLYLSARNVPKIDVQQAPNVDPISLIKFDKVIMTVDAIRKFEEVFK"
     gene            1685..1972
                     /gene="rplW"
                     /locus_tag="PK389_01175"
     CDS             1685..1972
                     /gene="rplW"
                     /locus_tag="PK389_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010133846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HQY22357.1"
                     /translation="MNEKRLYTIILGPHTTEKAVSVADQIAFRVAKDATKTELKEAVQ
                     KLFSVKVVSVRIVNVMGKTRQFRQRQGERSDWKKAYVRLDKGQAINLDAFQ"
     gene            1990..2823
                     /gene="rplB"
                     /locus_tag="PK389_01180"
     CDS             1990..2823
                     /gene="rplB"
                     /locus_tag="PK389_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019622819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HQY22358.1"
                     /translation="MVTTVVKMKPTSPGRRFVVKIVNPELHKGKPYGPLVVSKQQTGG
                     RNCYGRITAWQRGGGHAQRYRIIDWRRKKEGIPGKIERLEYDPNRSANIALVLYADGE
                     RCYMVAPKGLSVGSEILAGGEAPIAVGNCLPLRNIPVGSVVHCVEMKPGKGAQLARSA
                     GTSVQLLAREGAYATLRLKSGEVRRVLVECRATIGEVGNSEHNLRKLGKAGASRWRNK
                     RPRVRGVAMNPVDHPLGGGEGRTSGGRPPCTPWGIPEGKKTRRNKRTNKLIIRRRKAT
                     K"
     gene            2839..3165
                     /gene="rpsS"
                     /locus_tag="PK389_01185"
     CDS             2839..3165
                     /gene="rpsS"
                     /locus_tag="PK389_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HQY22359.1"
                     /translation="MPRSLRKGPFVDAHLLKKVEQAIATRIKKPIKTWSRRSMILPDM
                     VGLTIAVHNGRQHVPVLINENMVGHKLGEFAATRLFRGHSGDKTAKTVKKGGKDAGGK
                     DKGKGK"
     gene            3175..3504
                     /gene="rplV"
                     /locus_tag="PK389_01190"
     CDS             3175..3504
                     /gene="rplV"
                     /locus_tag="PK389_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015637230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HQY22360.1"
                     /translation="MLEVSAKLSDARLSPQKARLVADLVRGLPVGRALNILSFSPKKA
                     AQILKKLLVSAIANAEHNNALDVDELSVARIFVNEAPRLKRSTQRAKGRGNRIVKRNC
                     HITVVVA"
     gene            3525..4211
                     /gene="rpsC"
                     /locus_tag="PK389_01195"
     CDS             3525..4211
                     /gene="rpsC"
                     /locus_tag="PK389_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HQY22361.1"
                     /translation="MGQKVHPIGIRLGYIRESSSKWFPESGKSGGSFAKILASDLEIR
                     AYAAKKLPEAAGVSQVLIERTGRNVRITLKTAKPGVIIGKKGEDIEKFRADISAKIGF
                     PTDRIHINIEEIRKPELDATLVARSIAQQLERRIMFRRAMKRAVTSALRQGAKGVKVY
                     VSGRLGGAEIARSEIYREGRLPLHTFRADIDYGTAIAKTTYGVIGVKVWIFKGEILTG
                     KPQQSTQEVA"
     gene            4227..4640
                     /gene="rplP"
                     /locus_tag="PK389_01200"
     CDS             4227..4640
                     /gene="rplP"
                     /locus_tag="PK389_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HQY22362.1"
                     /translation="MLQPKRTKFRKQFKGHNRGVATRGNKVSFGEFGLKAMDHARITA
                     RQIEAARRAISRYIKRGGKIWIRIFPDKPITQKPLEVRQGSGKGNVEYWVALVQPGRV
                     LFEIEGVTPELAAEAFRLAASKLPVATELVPREIM"
     gene            4643..4837
                     /gene="rpmC"
                     /locus_tag="PK389_01205"
     CDS             4643..4837
                     /gene="rpmC"
                     /locus_tag="PK389_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019390273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HQY22363.1"
                     /translation="MKAAELVGKSIGELNTTLTTLRRKLFKLRLVKASGELVKTHEIK
                     ETRRNIARVTTMIAKEGKKT"
     gene            4834..5106
                     /gene="rpsQ"
                     /locus_tag="PK389_01210"
     CDS             4834..5106
                     /gene="rpsQ"
                     /locus_tag="PK389_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008488663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HQY22364.1"
                     /translation="MSERLSQGRTEQGVVVSDKMDKTITVRVVRKVKHPLYGKIMTRS
                     TKYHAHDPEGLCKEGDWVVIGESRPISKTKSWVLVERLEKIEREVL"
BASE COUNT         1536 a          890 c         1413 g         1299 t
ORIGIN      
        1 cgtgaaggcg gtcgcaccgt gggtgcaggt gttgtatcca aggtactgga gtaattttaa
       61 atggcaagtc aagtgataaa gattcgttta aagtcgtatg atcatcggct gattgataaa
      121 tctgctaaag agatcgttga tacggctaag cgaacgggcg ctcaagtacg aggtcctgtg
      181 ccattgccta cacgtcatga gcgttttacc gtgttggttt cgccgcatgt cgataagaat
      241 gcgcgtgatc agtacgaaat tccggaacat aagcgttacg tcgagattgt agaaccaacg
      301 gaaaagacag tagacgcgct gatgaaatta gatttagctg ctggcgttga tgttcagata
      361 gctgttagtt aggggtagaa aaatgattgg tgtcgttgcg actaaatgtg gaatgacgag
      421 gattttcgat gcggaaagtg gggcatcgat ccctgtaacg gtagcgatag tggaacctaa
      481 ccgcattgta cagatgaaaa cgcttgagaa tgatggctat attgccattc aagtgactcg
      541 tggtgaagtt aaacccagtc gtattaccaa agctgaagcg gggcattttg caaaggcaaa
      601 cgtggttgct ggtaaagatt taatggaatt tcgtgcggaa gcagaagact gtaaagacct
      661 tactgtcggt agcgaaattg gcgctgattt ctttaaagtt ggtcaaaaaa ttgatgtggc
      721 aggactgacg cgaggacgtg gttttgcggg ttgtattgtt cggcataatt ttagttcaca
      781 gcgagcatct catggtaact cgttgtctca taatacaccg ggctccattg gtcaaaatca
      841 aacgccaggc agagtgttta aaggtaaaaa aatggctgga cagtatggta atacgcaacg
      901 tacggttcaa aatctagagg ttgttcgaat tgatttagag cgacatgtta ttctgattaa
      961 aggcggagtt cctggagcgc ccggcggaaa gcttattatt tatccggcgg tcaaacataa
     1021 tattcaatca aatgctcaat caacggttgc ggcctaagga gatatcgatg gaaatcgcat
     1081 tcgccgtgcc tagtcatgag gcacccaaaa aaggtttaga agtatctgct ataacgtttg
     1141 gaagagattt taacgaagcg ttggtacacc aggtagttac tgcatattta gcaggcgcgc
     1201 gttcgggaac gcgtgcgcaa aaaacgcgtg cagaagtgcg tggcgggggc gctaagccat
     1261 ggcgccaaaa aggaacaggt agagcgcgtg caggtaccat tcgaagtcca ttgtggcgag
     1321 ggggcggtcg catatttgct gcaaagcctc aggatcatag tcaaaaaatc aataagaaga
     1381 tgtatcgagg tgcgatgcaa gccattttat ccgagcttgt acggcaggat agattgcttg
     1441 ttgttgacgc gattgaaatt tcacagccaa aaacacgtga tttgttggcg aaattgaaag
     1501 cattgggatt aaaagaggtt ctgattgtta caggtcaatg ggatccgaat ttgtatctct
     1561 cggcacgtaa tgtaccgaag attgatgtac agcaagcgcc gaatgttgat ccgattagtt
     1621 tgattaaatt cgataaagtt attatgactg tcgatgcgat ccgtaaattt gaagaggtat
     1681 tcaagtgaac gaaaaacgct tatacacaat tatattaggg cctcatacca ctgaaaaagc
     1741 ggtgagtgtt gctgatcaga tcgcatttcg cgttgcaaaa gatgcaacta aaacagaact
     1801 gaaagaagct gtgcaaaaac ttttttcggt taaagtggta tcggttcgaa ttgttaatgt
     1861 catggggaaa acgagacaat ttagacaaag gcaaggtgag agatccgatt ggaaaaaggc
     1921 ttatgttcgt ttggacaaag gccaagcgat taacttagat gccttccaat aaataacagg
     1981 gtattcaata tggtaacaac tgtagtaaaa atgaaaccga cctcaccagg gcgtcggttt
     2041 gttgtaaaaa tcgttaaccc cgaattacat aagggaaagc cttatggacc tttggtcgtc
     2101 tcaaaacaac aaaccggtgg tcggaattgt tatggacgaa ttacggcttg gcagcgaggt
     2161 ggtggacacg ctcaacgtta tcggattatc gattggcgtc gtaaaaagga agggatccct
     2221 ggaaaaattg agcgtttgga atatgatcca aaccgcagtg cgaatattgc attggtactt
     2281 tatgccgatg gtgagcgttg ttatatggtt gcgcctaaag gattatcggt tggttcagaa
     2341 attttagcgg gcggtgaggc acctattgca gttggcaatt gtttaccgct ccgaaatata
     2401 ccggtaggtt cggttgtgca ttgtgtggaa atgaaacccg gcaaaggtgc gcagctggcg
     2461 cgcagtgcgg ggacttctgt tcaattgttg gccagagaag gggcttatgc aacgcttcgt
     2521 ttaaaatccg gcgaagttcg acgcgttttg gtagaatgtc gtgcaaccat tggtgaagtc
     2581 ggaaacagcg aacataattt acgcaagctc gggaaagcgg gtgcaagtcg atggcgtaat
     2641 aaacgtccga gagtgcgagg ggttgctatg aacccggttg atcatccgtt aggcggagga
     2701 gaagggcgta catcgggtgg tcgtccaccg tgtacacctt ggggtattcc agagggtaaa
     2761 aagacacgtc gtaataagcg cacgaataaa ctgattattc gtcggcgtaa agcaacgaag
     2821 taaaaggaga agggttccat gccacgttct ttgagaaagg ggccatttgt agatgctcac
     2881 ttgctaaaaa aagtagagca agcgatagca acgcgtatta agaagccgat taagacttgg
     2941 tctaggcgtt cgatgatttt gccagacatg gtgggtttaa caattgcagt gcataatggt
     3001 cggcagcacg tacctgtttt aattaatgag aatatggtcg gacataaatt gggtgagttc
     3061 gctgcaacgc gactttttcg aggacattcg ggtgataaaa ccgcgaaaac tgtgaaaaag
     3121 ggtggtaaag atgctggcgg taaagacaaa ggtaaaggta agtaggggat tgtcatgctt
     3181 gaagtgagcg ccaaattaag tgacgcaagg ctttcgccgc aaaaagcgcg attggtggct
     3241 gatctcgtga gagggttgcc agtaggtagg gcgttgaata ttttgagttt tagcccgaaa
     3301 aaggcagccc aaatcctgaa gaaattattg gtatctgcga ttgcaaatgc agaacataat
     3361 aacgcgttag atgtagatga gctttcggtt gcacgcattt ttgtaaatga agcaccgaga
     3421 ttaaaacgat cgacgcaacg cgctaaaggg cgaggaaatc gaattgttaa aagaaattgt
     3481 catattactg tagtggttgc gtaattagtt agagagaggt tttcatggga caaaaggtac
     3541 atccaattgg tattcgctta ggatacatcc gggaaagttc ttctaagtgg tttccggaaa
     3601 gtggcaaaag tggcggttcg tttgcaaaaa tattagcaag cgatctcgaa attcgcgcct
     3661 atgcagccaa aaaattacct gaagcggcgg gtgtgagtca ggttttgatt gagagaactg
     3721 gtcgtaatgt tcgcattaca ctcaaaacag cgaagcccgg tgtgattatt ggtaagaaag
     3781 gtgaggatat tgaaaaattt cgagccgaca tcagtgctaa aattgggttt cctacagatc
     3841 gtattcatat caatattgaa gagatccgca agccggaatt ggatgcgaca ctggttgcta
     3901 gaagtattgc acagcagcta gagcgcagaa ttatgtttag acgtgcgatg aagcgtgcag
     3961 tcacaagcgc attacgacaa ggtgctaaag gtgttaaagt gtatgtttct ggtcgtttag
     4021 gcggtgctga aattgcacgt agtgaaatat accgtgaagg tcgtttgcca ctacatactt
     4081 ttagagcgga tattgattat gggactgcca ttgcaaagac cacttatggt gtgattggcg
     4141 tgaaagtgtg gattttcaaa ggtgaaattt tgactgggaa gccacaacaa tctacacaag
     4201 aagttgcgta agccgagggg attatcatgt tacagcctaa gcgaaccaaa tttcgcaaac
     4261 aatttaaagg gcacaaccgc ggggttgcaa cccggggtaa taaggttagt tttggcgaat
     4321 ttggcttaaa agcgatggat catgcgcgta ttacggcgcg tcaaattgag gccgcaagac
     4381 gtgcgattag ccgatatatt aaacgcggtg ggaaaatatg gatacggatt tttccagaca
     4441 agcctattac gcagaagcct ttagaagtcc gtcaggggag tggtaaagga aacgtagaat
     4501 attgggtggc tttggtacaa ccaggtagag tcctatttga aatagaaggg gtgacaccgg
     4561 agttggctgc agaagccttt aggttagcgg cttccaagct tcctgttgca acggagcttg
     4621 ttccacggga gattatgtaa tcatgaaagc agcagaactg gttggaaaat ccattggcga
     4681 gttgaataca acgctaacaa ctttacgacg taagttattt aaattgcgac tagtaaaagc
     4741 gagtggagaa ttagtaaaga cgcatgaaat taaagaaacg cgtcgaaata ttgcgagagt
     4801 aacgacgatg atagcaaaag aaggtaaaaa gacatgagtg agcgattaag ccaaggtcgc
     4861 accgagcagg gagttgtggt cagtgataaa atggataaaa ccattactgt ccgcgttgtt
     4921 cgaaaagtga aacaccctct gtatggtaaa attatgaccc gttccactaa atatcatgca
     4981 cacgatcctg aaggtctgtg caaagaaggg gattgggttg ttattggaga atccagaccg
     5041 atttcaaaaa caaaaagttg ggtactggta gaacggcttg aaaaaataga gcgagaagtg
     5101 ttatgatttc cgactttgac ggaggcagca atgattca
//
LOCUS       DAORVT010000035         5133 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_11287_length_5133_cov_3.394053, whole genome shotgun sequence.
ACCESSION   DAORVT010000035 DAORVT010000000
VERSION     DAORVT010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11287_length_5133_cov_3.394053"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..418
                     /gene="ruvX"
                     /locus_tag="PK389_01215"
     CDS             2..418
                     /gene="ruvX"
                     /locus_tag="PK389_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HQY22365.1"
                     /translation="MIGFDFGLRHIGVAIGQSVTQTATPLTTLAARDGVPNWLEIEGI
                     LEKWHPDDLIVGIPLHLDGTTQPMTFCARRFMNKLRTRFKIPTHAVDERLSTWEAKTR
                     AHTKIIHHPSKAKAHINEMHAYAAVVLIEQWMVDQQ"
     gene            complement(480..1181)
                     /locus_tag="PK389_01220"
     CDS             complement(480..1181)
                     /locus_tag="PK389_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22366.1"
                     /translation="MIKNTILKSFAVTLVLLATTAEAWTVSEWETDSAQFEKRETPLT
                     TDEKASGDYRQALKYRDNGEFLHAERLFKTVLAEMPTHYAARAELATLYLKRNQLDET
                     EMLLSEGLRLDENNPDFLRLMAVIYDKREEPDKALALLVKIKDSRKDKNYVAFLGHIY
                     QQTGRFALARQQYYRLLQEEPENALWLLGVSLALDAEGQKEVALEGYIKITADGNMDP
                     HVLQYIQKRIKALKG"
     gene            1527..2441
                     /locus_tag="PK389_01225"
     CDS             1527..2441
                     /locus_tag="PK389_01225"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HQY22367.1"
                     /translation="MIKRVKIIGLVGLLLGLSFPAWGLTFDWPAGTDIVGEIQTTTVR
                     KGESLGDIGRRFDVGVYEMIEANPRLDPWGPTVGAIVVIPTQFILPNVPRVGLVLNLA
                     EMRLYYFDLDNRQVTTHPLGIGKKGCPTPLGTTTVINKRKDPPWIPPDSIRKEHIAKN
                     DPLPAFVAGGVPENPMGRYALYLGAELGAGAYRIHGTNASGGIGVRGTHGCMRLLPED
                     IENLFYKVRIGTPVRIIHEPFKIGWHEGHLYLEAHEPCSEPKFAGSDSLSRLEKMIRN
                     AIESTHLVNWSSAKMGAKKANGYPIRID"
     gene            2631..>5133
                     /locus_tag="PK389_01230"
     CDS             2631..>5133
                     /locus_tag="PK389_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22368.1"
                     /translation="MKTPYGIAKMLSSDSYVRRPAVETKEKEHIAPASQVVRNSGLYH
                     FVATLRSRSIASPLDGFKVIPLETVSKEYEGDQVYDYTRKAYVVNFPALAPKDFLDEK
                     DCFELKYTHFIEHHLDIDALFAPGSSSAAQNRFPYCHYTQNFQTPDGSYITVHLHISR
                     NGKIEHFRVKSVRKITGADGETFENIENYPENILKQLQPWVEKHAEIALQLIKKLNQQ
                     KLDEILAIHDKINKSDHVLSGLFLELHNDTARRKYLRVAKKLTENINKLNKLSDSGYI
                     DVRNRHPEQICSHINAGKFTPQTVDSIQEDSAEIPEAVSEEPTAKEHKLAPSSKHSQK
                     PEDKFKQKIDAQRKQLLTELEGKEKQLDKLQVKKTLSAAELIARNQVADELCSITLSL
                     YCLSKNDQVTIKRLNNVEGKLANVPSLLQEFEKLAKLGELDKVSEIFPHIESSLNASF
                     LIDLLDDVIWKKNPKHLEKQIEVCNFLHKKSDLYRVAFEKVNQTFNAREDDNLALSQL
                     VLTSDKQNKLAFEMLLRQGVEPNAIGGIFGETLIPQTFTTLMTFQENPDIYYIQTLLA
                     YGASTELPKNYFNNLSYAYHHVRSDRVQHINPNPNVDPATLFEYKMLSNATSILYVAC
                     RYSPTKELIALLAPKSSLQGLLLSLAYFSFARKIEMQFLSTSTEAGLQVSVTGIECEN
                     KAVLETLQGTNDVPASMFLLNIADESDESLKGIMEIMVGEIKRKMLEHTPEEIQKLKE
                     DLMSLTKEERDTNDFFEKRRSYSACLYLHAFNPKPCIADKKDMVATFVGLVSTFLKFL
                     DTSNINRIQRLTQIQGYCVWAQELADAVITREPKFA"
BASE COUNT         1632 a         1011 c          987 g         1503 t
ORIGIN      
        1 tattattggc tttgattttg gcttacgaca tatcggcgtt gccattggac aatccgttac
       61 tcaaacagca acccctctca caacactggc agcgcgtgat ggcgttccta actggctcga
      121 aattgaagga attttggaaa aatggcatcc cgatgatctt attgtgggca ttccactgca
      181 tttagatgga acaacccaac ctatgacatt ttgtgcaagg cgttttatga ataaactgcg
      241 cacacgattt aaaattccaa cccatgcggt ggatgaacgc ttaagcacct gggaagccaa
      301 aacgcgtgca cacactaaaa ttatacatca tccctccaaa gcaaaagcgc atataaatga
      361 gatgcatgct tacgcagcgg ttgttttgat tgagcagtgg atggtggacc aacaataatt
      421 gattctttca aaaccgaaca ccaacaagtt gatctgtgtc tgaacttgct cccttttttt
      481 tatcctttaa gcgctttaat tcgcttttgt atatattgca gcacatgagg atccatattg
      541 ccatctgcag taatttttat atacccctcc aatgccactt ctttttgccc ttccgcatct
      601 aaggcaagcg acacgcctaa taaccacaat gcgttttcag gctcttcttg cagcaaacgg
      661 tagtattgct gtcttgccaa cgcaaaacga cctgtttgct gataaatatg ccccaaaaat
      721 gccacataat ttttatcttt tcggctatct tttatcttaa cgagtaaagc caaggcctta
      781 tcgggttctt ctcgtttatc gtagatcact gccatgagcc ttaaaaaatc aggattattt
      841 tcatctaacc taagtccttc ggataaaagc atttcggttt catctaattg atttcttttt
      901 aaatataaag tagccagctc tgctctggcg gcataatgtg ttggcatttc tgccaacacc
      961 gttttaaaaa gcctttctgc atgtaaaaat tcgccattgt cccgatattt taaagcttgt
     1021 ctataatctc ctgaggcttt ttcatctgtt gttaatggcg tttctcgctt ttcaaattgt
     1081 gccgaatccg tctcccactc ggagactgtc caagcctctg ccgtggtagc caataaaaca
     1141 agagtaactg caaagctttt tagaattgta ttttttatca tgtttgacaa tctcctgacc
     1201 gattatcctg tgccttgggt tattatctac ggtttcatga gtagatacca actacgcctt
     1261 ctttttggtt tttttgtgtt ttacaactaa aattccattc caaaatgctt tcctctttct
     1321 tttccacgct attcattgta gtatcgctgc taactagcgc aagactcttg aaaaagggtc
     1381 tgaagggacc gagtcttgat gatacggcct tcgaaatgaa tcattaaact aacttgatta
     1441 gagtgaatca aaggctataa caatcaagat ctgtctcatt tctgccgaca cataaactaa
     1501 gtgcggaaag agcgggatta aaaccgatga taaaaagggt caaaataatt ggattagtag
     1561 gcttattgtt aggtctaagc tttcctgcct gggggttgac gtttgactgg ccagctggca
     1621 ccgacattgt cggcgaaata caaaccacaa ccgttcgtaa aggagaatcc ttaggcgata
     1681 taggtagacg ttttgacgtt ggagtctacg aaatgataga agccaaccca agactagatc
     1741 cgtgggggcc gaccgtcggc gcgattgtgg tcatacctac gcaatttatc ttacctaatg
     1801 tgccgcgtgt gggcttggtt ctaaatctcg ctgaaatgcg cctgtattac tttgatctag
     1861 acaaccgtca ggtgacaact catccattgg gcattggcaa gaaaggatgc cctacccctt
     1921 tgggtacaac tactgtgatt aataaacgga aagatccgcc atggatccct cccgattcaa
     1981 ttcggaaaga gcatattgca aagaatgatc cattaccagc cttcgttgct ggcggtgtgc
     2041 ccgaaaatcc tatgggtcgc tatgcgcttt atttgggagc agaactcggc gccggtgcgt
     2101 atcgaattca tggaacaaat gcgtctggtg gcatcggtgt acgtggaaca catggttgta
     2161 tgcgtctatt gccagaagat attgaaaatc ttttctataa agtacgcatt ggtacacctg
     2221 tacgaattat tcacgaaccc tttaagattg gctggcacga aggacatttg tacttagaag
     2281 cccatgaacc ttgctcagag ccaaagtttg cgggttcaga tagcttatca cgattagaga
     2341 aaatgatacg aaacgctatt gaaagcacgc atctcgtgaa ttggagcagt gccaagatgg
     2401 gtgctaaaaa ggcaaatgga taccccatta ggattgatta gttttccttt atcttttttt
     2461 gcgtgtaatt actattaaaa aatagcttga tttttttacc taacttttat attgtgatta
     2521 cggtagctga aatcaaggtt ctgttctaca gtgcaataag ctctggaata acctcggaaa
     2581 gttgtcccaa aaatcattta taacgactaa ttaattttgg agaatgttca atgaagactc
     2641 cttatggaat agccaaaatg ttatcatcag attcttacgt tagacgacca gcagtagaaa
     2701 ctaaagaaaa agagcatatt gctcctgcat cgcaagtcgt tagaaatagc ggtttatatc
     2761 attttgttgc gacactccgc tcaaggtcaa tagcgagccc cttagatggt tttaaggtaa
     2821 taccattaga aacagtttca aaagaatatg agggagatca ggtttacgac tatactcgaa
     2881 aggcttatgt cgtaaatttt ccagcattag ctccaaaaga ttttttagac gaaaaagatt
     2941 gctttgaatt aaaatatact cattttatag agcatcattt agacatagat gcactttttg
     3001 cacccggttc ttcatctgca gcacaaaatc gatttcccta ttgccattat acacaaaatt
     3061 ttcaaacacc tgatggcagt tatattacag tgcatctaca cattagtaga aatggtaaaa
     3121 tagaacattt tcgagtaaaa tccgttcgaa aaataacagg agcagatgga gaaacatttg
     3181 agaacattga aaattacccc gaaaacattt tgaagcaatt gcagccttgg gtggagaagc
     3241 acgctgaaat tgccttgcaa ctaatcaaaa aattaaacca gcaaaaactt gatgaaatat
     3301 tagctattca tgataaaatt aataaatctg accatgtatt aagtggctta tttttggaat
     3361 tacacaacga tacggcaaga cggaaatatt tgcgagtggc taaaaaactt actgaaaata
     3421 ttaataaatt gaataagcta agtgatagtg gctacatcga tgttagaaat cggcaccctg
     3481 aacagatatg ttcgcacatt aacgcaggta aattcacgcc acaaacagtg gactcaatcc
     3541 aagaggactc ggcagaaata cctgaagcag tgagcgaaga gcctaccgcg aaagaacata
     3601 agctggcacc ttcttcaaaa cactcccaaa aacctgaaga taaattcaaa caaaaaattg
     3661 atgcgcaacg caaacaattg ctaacagagc ttgaggggaa agaaaaacaa ctagacaaat
     3721 tgcaggtaaa aaaaacgcta agtgcagcag aattgattgc ccgtaatcaa gttgccgatg
     3781 aattgtgctc tattacattg tctctttatt gtctttcaaa aaatgatcag gttacaatta
     3841 agcgtcttaa caacgtcgaa ggaaaattag caaacgtccc aagcttgtta caagagtttg
     3901 aaaaacttgc gaaattgggt gaacttgata aagtttcaga aatctttcct catattgaaa
     3961 gtagcttaaa tgcttccttt cttattgact tactagatga tgtcatttgg aaaaaaaatc
     4021 cgaaacattt agaaaagcaa atagaagttt gcaacttttt acataaaaaa tcagatttat
     4081 atagagtagc atttgaaaaa gttaaccaaa ctttcaatgc acgagaggat gataatctcg
     4141 ctttatctca attggtccta acatctgaca aacaaaataa gctggctttt gaaatgttat
     4201 tacgtcaggg tgttgaacca aatgcgattg gtggcatttt cggcgaaaca ttgattccac
     4261 aaacattcac gactctaatg acgtttcagg aaaatccaga tatctattat atacaaactt
     4321 tattagcgta tggcgcttct acagaattac caaagaatta ttttaacaat ttgagttatg
     4381 cgtatcatca tgtcaggagc gacagggtgc aacatataaa cccaaatcct aatgttgacc
     4441 ccgcaacgct ttttgagtat aaaatgttat caaatgcaac ttctatttta tatgtagctt
     4501 gccgatattc tccaaccaag gagttaatcg cgcttttagc acccaagagc agtctgcaag
     4561 gcttacttct ttccctagct tatttttctt ttgcaagaaa aatagagatg caattccttt
     4621 cgaccagcac tgaagctggc ttacaggtat ctgtcactgg catagaatgt gaaaataaag
     4681 ccgtccttga aaccttacaa ggtacgaatg atgtccctgc ttctatgttt cttttaaata
     4741 ttgcagatga aagcgatgaa tctcttaaag gaatcatgga aattatggtc ggggaaatca
     4801 agcgtaaaat gttagaacat actccagaag aaatacaaaa gttaaaagaa gacttaatga
     4861 gtttaacaaa agaggaaaga gatacaaacg atttttttga aaaaagaaga tcgtatagcg
     4921 cttgcttgta tttacatgct tttaatccaa aaccatgcat tgcggataaa aaagacatgg
     4981 ttgcaacttt tgtaggttta gtaagcactt tcttaaaatt cttagatact tctaatataa
     5041 atagaattca aagattaaca caaatccagg gctattgtgt ttgggctcag gaacttgcag
     5101 acgcggtaat cacaagagaa ccaaaatttg cac
//
LOCUS       DAORVT010000036         5091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_11446_length_5091_cov_3.397339, whole genome shotgun sequence.
ACCESSION   DAORVT010000036 DAORVT010000000
VERSION     DAORVT010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11446_length_5091_cov_3.397339"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="PK389_01235"
     CDS             <1..641
                     /locus_tag="PK389_01235"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HQY22369.1"
                     /translation="EGLTRNLPAFARFLDSLVFSNGMLVNYSNIARECAIDAKTVKEY
                     YQILIDTLVGYFVEPYTKKKGRDVISATPKFYLFDVGVASFLAKQTISALKGSEAGNA
                     FEHFILMELMGYRGLNDLDFDINFWRTKTKLEVDFILDGGRIAIEVKIDDNVKKQDLR
                     GLVAFCDEHKPEQAYVVSRDARPRKMTTVAGREILILPWEIFLKKLWNKEII"
     gene            complement(744..1040)
                     /locus_tag="PK389_01240"
     CDS             complement(744..1040)
                     /locus_tag="PK389_01240"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HQY22370.1"
                     /translation="MPDFWVYILYCKNNHFYVGYTTDLIQRYRLHSKGKAAKYTRSFP
                     PCYLAQIWPIYGSKKQAMQLERILKKMQRSQKKVVVESPEILERLILNQSIELD"
     gene            complement(1068..1313)
                     /locus_tag="PK389_01245"
     CDS             complement(1068..1313)
                     /locus_tag="PK389_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017838943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HQY22371.1"
                     /translation="MIKAALGSLQATVLTVEDQSAHHAGHAGAQSGGGHFALTIVSDT
                     FEGKTAVRRHQMIYEALGDLMKEDIHAISINAKTSHE"
     gene            complement(1344..2264)
                     /locus_tag="PK389_01250"
     CDS             complement(1344..2264)
                     /locus_tag="PK389_01250"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQY22372.1"
                     /translation="MKKLSLGYVELSLAQCCIGINIILGKTLIPEIPIFALLLLRFTL
                     GFVFVGTYQMFGNFSLVRTELSRLSPKDWLIIFTQALCAGFLFNVLILYGLEHTTATV
                     TGIINSAVPALVAVFSFIILHEKLTLRIGIAILLCIVGIAILGFGKAIPTDSNQNELL
                     GILLVLAAIVPEALFTVLAKLLKKPPSPLTTTLLINFFNMILFIPFAITAEWGQVKDA
                     SMLTISQIFLYGLTGGILFFVFWYRGLSCISANTAALFMGIMPISTCILAYFVLNEIL
                     GLDDLIGMLLVIISIIIGTRHPMKKHKVKE"
     gene            complement(2314..2865)
                     /locus_tag="PK389_01255"
     CDS             complement(2314..2865)
                     /locus_tag="PK389_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_819923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HQY22373.1"
                     /translation="MKFLFDFFPIVLFFIAFKMGGIYIATITVIVASAAQVLWSRYKT
                     GRFEKLPLITLGTIVVLGGATLFFKNELFIKWKPTALYWVLALALVISQFIGNKPLMQ
                     RLLEQNVVLPTKIWHQLNLSWALFFAAMGIANLYVVYHFNTDTWVNFKLFGTLGLTLV
                     FIVLQGFYMAKYFKQPVSVKNQE"
     gene            3088..4125
                     /gene="trpS"
                     /locus_tag="PK389_01260"
     CDS             3088..4125
                     /gene="trpS"
                     /locus_tag="PK389_01260"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HQY22374.1"
                     /translation="MNSLNSKNSHFRRSRVVSGIRTTGALHLGNYHGALKNWVNLQSK
                     YDCFFFAADWHALTTEYEHPERIAQYTSEMILDWLGAGLDPESCTFFVQSHVPEHAEL
                     QVLLGMLTPLSWLERVPSYKELRSKLKEKHLATYGFLGYPVLQSADILMYKPEFVPVG
                     EDQVSHIELTREIARHFNHLYEERLQKPFFPEPKALLTETAKMPGLDGQKMSKSYHNT
                     IGLREDPKSVEHKIKTMPTDPARVRRTDPGEPEKCPVWRFHQVYSSKETQEWVQTGCR
                     TAGIGCLECKKPVIAAVLEEQAVIINRVEPYAKNPGLVQDIIKEGCKVARQAAGQTLT
                     EVREVMGLLKT"
     gene            4215..4943
                     /locus_tag="PK389_01265"
     CDS             4215..4943
                     /locus_tag="PK389_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008285093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HQY22375.1"
                     /translation="MDLYIPPDAMEVVLEIFEGPLDLLLYLIKKHNLDILNIPVASIT
                     RQYMEYVEVMSVLRLELAADYLEMAALLAEIKSRMLLPRPVEEAGQDLDPRAELVRRL
                     QEYERIKTAAENLDQLPRMERDWIPVSADVSKLELVRPHPSVELKEVLLSFKEVLRRA
                     DLQTMHMIQREVLTVREKMSRILSNLEGEAFLDFVSFFDMSEGRLGIVVTFLAILELV
                     RETMIELVQSEPFGPIYIRAAGCQ"
BASE COUNT         1602 a          897 c         1115 g         1477 t
ORIGIN      
        1 ccgaagggtt aacacgaaat cttcccgcgt tcgcacgttt tttagattct ctagttttta
       61 gtaatggtat gcttgtcaat tattccaata ttgccagaga atgcgcaatt gatgcaaaaa
      121 ccgttaaaga atattaccaa attctcattg atactttagt cggttatttt gtagagccat
      181 atacgaaaaa aaaagggcga gatgttatta gcgctacacc gaaattttat ttgtttgacg
      241 tgggggttgc tagctttttg gcaaaacaaa caatttcagc gttaaaagga agcgaagcag
      301 gcaatgcatt tgagcatttt attctaatgg aattaatggg atatcgcggg ttaaatgatt
      361 tggattttga tattaatttc tggagaacta aaacgaaatt agaagtcgat tttatcttgg
      421 atgggggtag aattgcaata gaggtaaaaa ttgatgacaa tgtaaaaaag caagatttaa
      481 gaggtcttgt tgctttttgt gatgagcata aacccgagca agcatatgtg gtttcaagag
      541 atgcccgccc acgaaaaatg acgactgtgg caggtagaga aatcttaatc ttaccgtggg
      601 aaatattttt aaagaagtta tggaacaaag aaataattta aatggataga acaagaacac
      661 ataaccctct ccggcgttcg cgggagaggg tggcgctaag cgccgggtga gggaggtgag
      721 gggaagggaa cggtatggag cgtctaatca agctcaatgc tctggtttag tatgagtcgc
      781 tctaaaattt cggggctctc aacaacaacc tttttttgcg atcgctgcat tttttttaag
      841 atccgttcaa gttgcatcgc ctgtttcttt gagccataga tgggccatat ttgcgctaag
      901 tagcacggtg gaaaacttcg ggtatatttt gctgctttgc cttttgaatg taaccggtat
      961 cgctgaatca aatctgttgt atagcccaca taaaaatgat tatttttgca gtataaaata
     1021 taaacccaaa aatcaggcac gttaattacg atggcttaac tatttgatta ctcatgggac
     1081 gttttggcat taatactaat ggcatgaata tcttccttca tgagatcacc taatgcctca
     1141 tagatcattt gatgtcgtcg cacagctgtt ttgccttcaa aggtatcgct gacgattgtc
     1201 agggcaaaat ggccgccacc actttgcgcg cctgcgtgac ccgcatgatg ggcgctttga
     1261 tcttccactg ttagtacggt agcttgcaag cttccgagcg cagctttaat cattgtgatt
     1321 ctatctttag cgtgtttcat aaattattcc ttgaccttgt gttttttcat gggatggcgt
     1381 gtgccaataa taattgaaat aataactaaa agcataccaa ttaaatcatc caaacccagt
     1441 atctcattta gcacaaaata ggctaagata catgtgctga taggcataat gcccataaat
     1501 agtgctgcag tattggcaga tatgcatgat aagccgcgat accaaaatac aaaaaatagg
     1561 attcccccgg tcaggccata gagaaaaatt tgagatattg ttaacataga agcgtctttc
     1621 acttgtcccc attcagcagt aatcgcaaac ggaataaata agatcatatt aaagaaatta
     1681 atcagtagcg tcgtggttaa aggagacgga ggttttttca ataattttgc taaaacagtg
     1741 aataatgcct cgggaacaat ggcagcaaga accaacagga tgcctaaaag ttcattttgg
     1801 ttcgaatccg ttggaatggc ttttccgaaa cccaatatcg ctatacctac aatacataac
     1861 aatattgcaa ttcctatcct cagcgttaat ttttcatgaa gaatgatgaa tgaaaaaacg
     1921 gcaactaaag caggtaccgc gctattgata atcccagtga cagttgcagt tgtgtgttct
     1981 aatccataca ggataagcac attgaataaa aatccagcac ataatgcttg ggtaaagatg
     2041 attaaccagt ctttagggga cagtctgctc agttctgtgc gtactaaaga aaaatttcca
     2101 aacatttgat aggttcctac aaaaacaaac cccaatgtaa aacgaagtaa taacaatgca
     2161 aaaatcggaa tttcagggat taaggttttt cctaatatga tattaatacc aatacaacat
     2221 tgtgctaagg aaagttctac atatcccaga gaaagctttt tcatgaccaa tctaccaaag
     2281 aaaacacgct agacttcctt agacttcctt tttttattcc tggtttttta cgctaaccgg
     2341 ttgtttaaaa tactttgcca tatagaatcc ttgcagcaca ataaaaacca gcgttaatcc
     2401 gagcgtgccg aataatttaa aattaaccca ggtatcggta ttaaaatggt aaacaacata
     2461 gaggtttgca atacccattg ctgcaaaaaa gagtgcccag cttagattga gttgatgcca
     2521 aattttagtg gggagcacta cgttttgttc caataagcgt tgcatcaggg gtttgtttcc
     2581 aataaattga ctaatcacta aggcgagcgc aagtacccaa taaagagcag taggtttcca
     2641 tttaatgaat aactcatttt tgaaaaacag cgttgcgcca ccgagcacca caatggtgcc
     2701 taatgtgatg agcggtagtt tttcaaatcg ccctgttttg taacgagacc acaatacttg
     2761 tgcagcactt gcgacaatga cggttattgt tgcaatataa ataccaccca ttttaaaagc
     2821 aataaagaaa agtacgatgg ggaaaaaatc aaataaaaat ttcatggttt atccctaaat
     2881 ttagtgtaaa atcaaacaac tcatttcgat tttagtgtat caagtttaaa cgtgcttgga
     2941 tagcaatcga aagaaaaaat aactaggtat aaaaacagct tttttgggtt tttggtgtta
     3001 aacttgcttg aatggcaata tcttcgtaaa gtgtcactat tctttcgtta tttggtttat
     3061 cagtgtaaat aggtttcgtg tgcaatcatg aatagtctta acagtaaaaa cagtcatttc
     3121 agacgctcta gagtcgtttc gggtattcga acaaccggtg cattgcattt gggtaattat
     3181 catggcgctt taaaaaactg ggtaaactta cagtcaaaat atgactgttt cttttttgcc
     3241 gccgattggc atgcgttaac cacagaatac gaacatccag agcgcatcgc acaatatact
     3301 tcagagatga tcttggattg gctgggtgct gggcttgatc cggaaagttg tacttttttt
     3361 gtgcaatcgc atgtgcctga acacgctgaa ttgcaagtac tgctgggtat gctaacgccg
     3421 cttagctggc tagaacgtgt gccgagttat aaagaattgc gcagtaaatt aaaagaaaag
     3481 catctggcaa cttatggttt tctaggatat cccgtattac aaagcgctga tattttaatg
     3541 tataaacccg aatttgtgcc cgtaggcgaa gatcaagttt cacatataga attaactcga
     3601 gaaatcgcga gacattttaa tcatttatat gaagaacgtt tacaaaagcc gtttttcccc
     3661 gagcccaaag cgcttttaac cgaaaccgct aaaatgccgg gtttagatgg tcagaaaatg
     3721 tcgaagtctt atcataatac gattgggcta agagaagatc cgaaatcggt ggaacataaa
     3781 attaaaacca tgccaacgga tcctgcgcgc gtcaggcgaa cagatccggg agagccggag
     3841 aagtgtccgg tttggcgctt tcaccaagtg tattcttcaa aagagactca agaatgggtg
     3901 caaacgggtt gcagaacggc aggcattggc tgtttagagt gtaaaaaacc cgtgatagct
     3961 gccgttttag aagagcaggc ggtgattatt aatcgtgtag aaccgtacgc gaaaaatcct
     4021 ggattggtgc aggatattat aaaagaaggt tgcaaagtgg cgcggcaagc ggccggacaa
     4081 acattgacgg aagttcgtga agtcatggga ttattaaaaa catgatgaat gaaatcatta
     4141 acgaatctgc tttaacacca gaagttgcaa cctgttatgg gtttgtacga ggcgaagcgg
     4201 ttacgcattt gcctatggat ctctatattc cgccagatgc aatggaagtt gtgctcgaga
     4261 tctttgaagg gccattggat ctgttgttgt atctgattaa aaaacataat ttagatattt
     4321 taaatattcc cgttgcatct attacacgac aatacatgga atatgtagaa gtgatgtctg
     4381 tgcttcgatt agaattggcg gccgattatt tagaaatggc ggctttgtta gcagaaatta
     4441 agtctcgcat gttattacca cgtcctgtag aggaagcggg acaagattta gaccctcgcg
     4501 cggaattagt ccgtcgtttg caagaatatg agcgtattaa aacagctgct gagaatttag
     4561 atcagctgcc tcgcatggag cgagactgga taccggtaag tgctgatgtg tcaaaattag
     4621 aattggtacg accccatcca tcagttgaat taaaagaagt tttactttcg tttaaagaag
     4681 ttttaagacg agcagatttg caaaccatgc atatgatcca gcgagaagtg ttaacagttc
     4741 gagaaaaaat gtcgcggata ttgagcaatt tagaaggtga agcattttta gattttgtga
     4801 gtttttttga tatgtcagaa gggcgtttag gtattgtggt tacttttctg gctattttag
     4861 agttggtaag agagaccatg atagaattag tgcaaagtga accatttgga ccaatctata
     4921 taagggcagc tggatgtcaa tagaaatcaa tttacaggcg ttattagagg ccattttgtt
     4981 agcggcagca aagcctttaa cagaagagca gttattgaat ttgtttgagg aagaagaaag
     5041 accgtctatc gatgtattac gcgaagcctt aggggatttg caaaatgcgt g
//
LOCUS       DAORVT010000037         5053 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_11613_length_5053_cov_3.683473, whole genome shotgun sequence.
ACCESSION   DAORVT010000037 DAORVT010000000
VERSION     DAORVT010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11613_length_5053_cov_3.683473"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="PK389_01270"
     CDS             <1..627
                     /locus_tag="PK389_01270"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HQY22376.1"
                     /translation="PQTKQAKIQKNNVACKQVPLFGRLRKNFNLTTQFTGMQNTPEVQ
                     KHFKKFNQSKSLFLKTTMLASSDLCYILEQVEKRKLPAELVLLPMLESNFQSQAISNR
                     GAVGLWQFMPATGRQFGLKQRSGYDGRKDVKASTHAALNYLEYLHKKFNRDWMLTLAA
                     YNAGEGTVERAIKRNKKAGKPTSFWFLALPKQTREYVPKFLALTQMIR"
     gene            678..1001
                     /locus_tag="PK389_01275"
     CDS             678..1001
                     /locus_tag="PK389_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQY22377.1"
                     /translation="MSIKTIDPRTLKAWLEKDEAFVIDVREPHEYHLAHIPGATLIPL
                     ATLSAEGLPDHGGKKRVLHCQHGKRGALACERLVIVDPRLEIYHLEGGLAAWLNAGYK
                     VSQSS"
     gene            complement(998..1642)
                     /locus_tag="PK389_01280"
     CDS             complement(998..1642)
                     /locus_tag="PK389_01280"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HQY22378.1"
                     /translation="MSVEFEKLIAGYSRFREKFKSGSHLLYKHLSEQGQRPSAIVIAC
                     SDSRVDPAMILDCDPGDLFMIRNVANLVPPYEDDHHYHGTSAALEFGVCHLKIAHIIV
                     LGHSQCGGIQSLLETNTVDPNGFIAKWMQLAKLSTRIQMSDDFLPIAHQAELCEKESI
                     SGSLDNLMTFPWIQKRVKENKIILHGWYFNLSNGTIDYFDQTTRQFLPLTQEQC"
     gene            1833..2744
                     /locus_tag="PK389_01285"
     CDS             1833..2744
                     /locus_tag="PK389_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006341999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQY22379.1"
                     /translation="MKNTLRPRYQTIMAVNDISFQVKAGERVAFIGPNGAGKSTTIKI
                     LTGIMQSTSGRVKVLGSDPAADRKCLAYQIGAVFGQRSQLWYHLPVQETLTLLAAIYD
                     IPKSEYQQRLKILAEKFNVTSLLTKPVRQLSLGERMRCEIIASFLHKPAVLFLDEPTI
                     GLDITAKAIIRDLILKQSEQEGVTLLLTSHDTDDIEKVCDRVIIIDQGQLLLDNSLHN
                     LKTQYIKKRVLSIVTEEERIICKLPGILVLEQSPHHLKIEIDIAQHAVESVVADLLEN
                     YTLRDLTIEGPPLEAIIQMLYANGKNK"
     gene            2728..3492
                     /locus_tag="PK389_01290"
     CDS             2728..3492
                     /locus_tag="PK389_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22380.1"
                     /translation="MAKTSNKYWASIDIAIRHQLAQRGQLVGRFFLYVVIVYLFHQVF
                     SSVQAPASRLWYLAITEWIVLSAPPLAFQLADDINSGQIAYFMLRPMHFLNLRLMACI
                     GAFTVRFVLLGLSCVGLGICLTGYFPTDVLTWLIGICFGILSVLLYNAILILIGLCAF
                     WLRDIKTLIYLNLTATLCLGGLIVPLSFYSPLVKTISFCTPYPWILWWPAEFMTGGHV
                     NVSLALTSWSVWMLLLWSVILFLYKKGIQSFVIEGG"
     gene            3493..4272
                     /locus_tag="PK389_01295"
     CDS             3493..4272
                     /locus_tag="PK389_01295"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HQY22381.1"
                     /translation="MFLLKFVWLTICTNLKLSFALRGAFFITLVVTITKQLLFLIAWS
                     FFFGRYKVVQGWTFEHMILAYGILCFAMGFVEAFFYGIKELPQLIETGHLDTFLLQPK
                     SIILNIALSKGDVSALGEVVTGILLVAYSGYFRSDLLIVVLLMVVSTFCMFALLLYLS
                     CIAFFMRNASDFIKELNLNAIIVATQPNAAYSGAFKIFTLTILPVAFLSYFPVEYFRT
                     GLWYYLLITFVGTGVFFGVAYGLFTCGLKRYESGNMMFTRQ"
     gene            complement(4335..4703)
                     /locus_tag="PK389_01300"
     CDS             complement(4335..4703)
                     /locus_tag="PK389_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007799021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY22382.1"
                     /translation="MLNPNLVLFYVKDPIESASFYEKIFEQQPVAAFPTYVAFRFSND
                     FTFSLWSTQAKDFVSLGSGNRSELAFMVSDENKVRSLRDRWGKLGVVIEQDLHEAVFG
                     LTFVALDPDGHRIRVCIPDK"
     gene            4785..>5053
                     /locus_tag="PK389_01305"
     CDS             4785..>5053
                     /locus_tag="PK389_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HQY22383.1"
                     /translation="MNRTERLLTLLKILRRYRKPVSGQKLADELNISIRTLYRDIVTL
                     QLQGADIEGEPGVGYILRPGFFLPPLMFSQTEMEALMLGILWVST"
BASE COUNT         1453 a          917 c         1080 g         1603 t
ORIGIN      
        1 ccgcaaacta aacaggcaaa aatacaaaaa aataatgtgg cctgcaagca agttcctctt
       61 tttggaaggt tgcgtaaaaa ctttaatctt acaacgcaat ttacggggat gcagaataca
      121 ccggaagttc aaaaacattt taaaaaattt aatcagtcaa aaagcttgtt tctaaaaaca
      181 acaatgcttg caagctcgga tttgtgttac attcttgaac aagtcgaaaa acgtaagttg
      241 cccgctgagc ttgtgttgtt gcccatgctc gagagcaatt ttcaatcaca agcgatttcg
      301 aatcgaggtg ccgttggatt atggcaattt atgcccgcta caggtagaca atttggttta
      361 aaacaacgct caggatacga tggtcgcaaa gatgttaagg cttccacgca tgccgcttta
      421 aactacctgg aatatttaca taaaaaattc aatcgcgatt ggatgctgac actggcggcc
      481 tacaatgcag gagaaggcac tgttgaacgc gccattaaac gtaataaaaa ggcagggaaa
      541 ccgacgtctt tttggtttct agcgcttcca aagcaaacac gcgagtatgt gccaaaattt
      601 cttgcattaa cgcaaatgat acgctagttt aatcaggtta gatttttttt ccgaaagata
      661 ggaagttgaa taccgttatg tcgatcaaaa caatcgatcc aagaaccctt aaagcctggc
      721 tagaaaaaga tgaagcattt gtgatagatg tacgagaacc acatgagtat catcttgcac
      781 acattcctgg cgctacttta atcccacttg cgacattatc tgcagagggg ttacccgatc
      841 atgggggaaa aaaaagagtg ctgcattgtc agcatggtaa gcgaggagct ctggcgtgtg
      901 agaggcttgt tatcgtagat cccaggttgg aaatctatca cctggagggt gggttggccg
      961 cctggctaaa cgcgggatat aaagtatcac aaagctctta gcattgttct tgagtgagcg
     1021 gcaaaaattg tcgtgttgtt tgatcaaaat aatcaatggt tccattggat aaattaaaat
     1081 accatccgtg caggattatt ttgttttctt ttactctttt ttgaatccat gggaaagtca
     1141 ttaagttatc gagtgagcct gagatcgact ctttttcgca aagttcagct tgatgtgcga
     1201 taggcagaaa atcgtcggac atttgtatac gagtggagag tttagctaat tgcatccatt
     1261 tagcgataaa gccgtttgga tcaactgtat ttgtttctaa gagagactgt attccgccac
     1321 attgactatg acctaataca ataatatgtg caatttttaa atgacagaca ccaaattcta
     1381 aggcggcgct ggttccatgg taatggtgat catcttcata agggggaact aaatttgcga
     1441 cgtttcttat cataaataaa tcacccggat cgcagtctag aatcatcgcg ggatctacgc
     1501 gagaatcaga acaagcaatc acaattgcgc ttgggcgttg accttgttct gataagtgct
     1561 tgtataataa atgagagccg cttttaaatt tttctcgaaa acgagaatac cctgcaatta
     1621 atttctcaaa ttcaacagac atattctatc ctatattgat tctgtcattt cgttgggcca
     1681 aaggccgata ttttagcaca gttcaggagc ctaagttatt gcttttatat gaccgtctag
     1741 ataagatatg cccaatgaac gccatcctag ttgaaaattt acgaaaatgc tttttaacac
     1801 ccttaccttc ggaagggtct tttttacaaa aaatgaaaaa cactttgcgt ccgcgttatc
     1861 agacgatcat ggcggttaac gacatttcct ttcaggtgaa ggcaggagag cgtgttgcct
     1921 ttattgggcc gaatggagcg gggaaatcca cgacgattaa aattttaaca ggcattatgc
     1981 agtcgaccag tggacgcgtt aaagtattag gtagcgatcc tgcggcagac agaaaatgcc
     2041 tggcttatca aattggagca gtgtttggac aacgttctca attatggtat cacttacccg
     2101 tccaagagac tttaacattg ttagccgcca tttatgatat tcctaaaagc gaatatcagc
     2161 agcgcttaaa gatactggct gaaaaattta atgttacttc actattgacc aagccggtgc
     2221 gccaattatc attgggtgaa cgtatgcgtt gtgaaattat cgccagtttt ttacataagc
     2281 ccgcagtctt gtttttagat gaacccacga ttggtttgga tattacagcc aaagcgatta
     2341 ttcgtgattt gatactcaag caatctgagc aagaaggcgt cacattgcta ttaacctcgc
     2401 atgatactga tgatattgaa aaagtctgtg atagggttat tattattgat caaggacaat
     2461 tactgctgga taacagtctg cataatttaa aaacacaata tattaaaaaa agagtgcttt
     2521 ctattgtaac tgaagaagaa agaattattt gtaaattacc cggtatatta gtattagagc
     2581 aatctccaca tcatttaaaa attgaaatag atatcgctca gcatgccgtc gagtcggttg
     2641 ttgccgactt gttagaaaat tataccctcc gagacctcac gatcgaagga ccaccgttag
     2701 aagccatcat tcagatgcta tatgcgaatg gcaaaaacaa gtaataaata ttgggctagc
     2761 atcgatattg ctatacggca tcaattggcg caacgtggtc aattggtggg tcgttttttt
     2821 ttatacgttg tgattgtcta tttgtttcat caagtcttta gtagtgtgca agcgcccgcc
     2881 tctcgattat ggtatttggc gattacagag tggattgttt tatcggcacc tcccttggcc
     2941 tttcagcttg ccgatgatat caatagcgga caaattgcat attttatgtt aagacccatg
     3001 cattttttaa atttgcggct aatggcatgt attggcgctt ttacggttcg ttttgtttta
     3061 ttggggctga gctgcgttgg tttgggtatt tgtttgacag gctattttcc aacggatgtt
     3121 ttaacctggc tcattgggat ctgcttcggt atactgagtg tactgcttta taacgcaata
     3181 ctcatattaa taggattatg tgctttttgg ctgagagaca ttaaaacgct catttattta
     3241 aacctaaccg caacattatg tttgggtgga ttaattgttc cactttcatt ttattctccc
     3301 ttagttaaaa ccattagttt ttgtacgccg tatccttgga tattatggtg gccagcggaa
     3361 tttatgacgg gtggacatgt gaatgtgtct ttggcgttga cgagttggag cgtttggatg
     3421 ctgttgttat ggagcgtgat tcttttcttg tataaaaaag gcattcaatc atttgtgata
     3481 gaaggaggat aaatgtttct gttaaagttt gtatggttga ccatctgtac gaatcttaag
     3541 ctttcttttg ccttgcgagg cgcttttttt attacattag ttgtgacgat tacaaaacaa
     3601 ttactttttt tgatagcgtg gagttttttt ttcggtagat ataaagtggt ccaaggctgg
     3661 actttcgaac acatgatttt agcgtatggg attttatgtt ttgcaatggg gtttgtcgaa
     3721 gctttttttt atggcataaa agagctgccg cagcttatcg aaaccggcca cttagatact
     3781 tttttgctgc aacccaaaag cattatattg aatattgcct tatctaaagg tgatgtttcg
     3841 gcattgggag aggttgttac aggtatttta ttagtcgcat attcaggata ttttagatcg
     3901 gatttactga ttgtggtgtt attgatggtt gtttcgacat tttgtatgtt tgcattattg
     3961 ttgtatttaa gttgcattgc cttttttatg cgaaacgcct ctgattttat taaagagctg
     4021 aatttaaacg cgattattgt ggcaacacaa cccaatgcag catatagcgg tgcgtttaaa
     4081 atatttacct taactatttt gcctgtcgca tttttaagtt attttcctgt tgagtatttt
     4141 agaacggggc tttggtatta tttattgata acgtttgttg gtacaggtgt attttttggc
     4201 gtagcctatg ggttatttac atgcggctta aagcgttatg aatcgggaaa catgatgttt
     4261 acgcgtcaat gagtgtgtgg attattttaa atcccccttt tttcaaaggg gaggcttaac
     4321 ttttttcgtc tgtgctactt gtcgggaata caaacacgaa tacgatgacc atcgggatct
     4381 agggcgacga aagttaatcc aaatactgcc tcatgcagat cttgctctat aaccacgccc
     4441 aattttcccc aacgatcacg aagagaacgc actttattct catctgaaac cataaaagcc
     4501 aattctgatc gattgcccga tccaagcgaa acaaaatcct ttgcttgtgt cgaccagagg
     4561 ctaaatgtga aatcattaga gaacctgaag gcaacatagg ttggaaaagc ggccactggt
     4621 tgttgttcaa aaattttttc ataaaaagat gcgctttcta tcggatcttt tacataaaag
     4681 agcacaagat ttggatttaa catgactata ttctcctgcc attaaggtat catcgtaata
     4741 atatacaata tgacactgcc aaaaaatggc agtatcatat atttatgaat cgaacagaac
     4801 gccttttgac attactcaaa atcctaagaa gatatagaaa gccagttagt gggcaaaaac
     4861 ttgcggatga gttaaacatc agtattcgca ccctgtatcg cgacatagtg acgcttcaat
     4921 tgcaaggtgc tgacatcgaa ggagaacctg gcgttggcta catacttcgg ccaggctttt
     4981 ttttaccacc attgatgttc tcgcaaacag aaatggaagc actgatgctt gggattcttt
     5041 gggtatctac att
//
LOCUS       DAORVT010000038         5021 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_11746_length_5021_cov_3.099678, whole genome shotgun sequence.
ACCESSION   DAORVT010000038 DAORVT010000000
VERSION     DAORVT010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5021
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11746_length_5021_cov_3.099678"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PK389_01310"
     CDS             complement(<1..540)
                     /locus_tag="PK389_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HQY22384.1"
                     /translation="MSEAPVEHLSGSVERVTFHNEDSGFCVLKVKVKGQRDLVTVIGS
                     SGTVGPGEYIDCSGTWVNNREYGLQFKADHLRIVLPSTLEGMEKYLGSGLVKGIGPHF
                     AKQLIQAFAMQVFDIIENTPDRLMELSGIGISRRDSVVKSWQDQKMIREIVVFLQSHG
                     IGTARAVRIYKTYGQQSIAK"
     gene            complement(550..975)
                     /locus_tag="PK389_01315"
     CDS             complement(550..975)
                     /locus_tag="PK389_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22385.1"
                     /translation="MFDMVLAGVYRCVDKNGIAEFRDRGCELASGEDDFLPYVYKPTD
                     PNIVLEKEGELHNTQKATQKQLLLQEKKMRLEARQKKTAEKAAALAERRLMRCEKTSE
                     KIKQIETELRTGCKIRRSNTLKKQLIHAQKMQKHYCTPP"
     gene            complement(1035..1727)
                     /gene="ung"
                     /locus_tag="PK389_01320"
     CDS             complement(1035..1727)
                     /gene="ung"
                     /locus_tag="PK389_01320"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007801219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HQY22386.1"
                     /translation="MSKDYIECSMLEESWRYALGTEFDKPYMRQLRLFLRQEKDQKKI
                     IYPKTSDVFHAFALTPLAQVKVVILGQDPYHGPGQAHGLCFSVQPGVRLPPSLVNIYK
                     ELQADLGIPPAQHGCLVNWAKQGVLLLNSVLTVEQGRAASHQGHGWEQFTDRVIEILN
                     ETMEGIVFLLWGAYAQRKGQFIHANRHCVLKTTHPSPFSAHQGFLGCKHFSKTNQYLQ
                     SLKKEPIDWRLA"
     gene            1746..2426
                     /gene="mutH"
                     /locus_tag="PK389_01325"
     CDS             1746..2426
                     /gene="mutH"
                     /locus_tag="PK389_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008109258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HQY22387.1"
                     /translation="MLKLTPPKTEAELLSRCYQLSGITFSALAQRYHQKLPSSLTQAK
                     GLIGQLIEYALGASAGNLPEPDFPNLGIELKTIPIDANGHPRETTYVCTAPINLKAEI
                     ESWEDSRVRKKLARVLWVPVEADPKIPLPDRCIGNAQLWSPDQHTETTLRQDWSELTH
                     MLHLGQIDNLSAKIGTYLQIRPKAAHSRILNPGINEHGEEILINPKGFYLRTSLTQKI
                     LQYCYCKA"
     gene            2558..3751
                     /locus_tag="PK389_01330"
     CDS             2558..3751
                     /locus_tag="PK389_01330"
                     /inference="COORDINATES: protein motif:HMM:NF017347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HQY22388.1"
                     /translation="MPIETNTKNLKPAAGNASETKIVITTGLALFAMFFGAGNILFPL
                     YLGANAGQNIGVITLGFLIAGVGVPFLGLIATSLFNGSYHAFFARLGKTPGFLLITFL
                     IIIIGPLGAIPRTEVTTFHALQPYLPHFLDNNAIFSLFYCGLVFLLAYREAKVVEILG
                     LYLSPVKIVSFTALIILGFISVEPAMTSPASTFQAFNKAIMNGYNTMDLLGAFFYCAV
                     AFKSIKQAIHHNPALNPTTLTLRSSIIGAGITGAVYIGFMWIAHNHAASLQGIPEEKM
                     ISAISMLVLGKFGAIFVCVSVSFACVATALALADVCTIYLYNEVFHKRVSKIACLSTV
                     MILTYLMTNIGFQGILSFTVPILNIVYPALIVLCICNILHKWKGIKMVKLPVLLTILI
                     FVVIC"
     gene            3874..>5021
                     /locus_tag="PK389_01335"
     CDS             3874..>5021
                     /locus_tag="PK389_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22389.1"
                     /translation="MLSQAHIDALAKSKKIISARMLYNKVQLKFLLDPKKSKLASALF
                     NISYPQFEGTEQFIKATFVEVRNYNIYIEYIETCRTVAAFDDLYFQFLIESRSQLSNV
                     DRVNKRILKSKRIPGMKKSQSIEESETFFEDNTELKYYKEIFDILRKHISQCSYEFTQ
                     HFNFSYTTIEKNPILQIDYSPLGGALFTIVKKLNKRLRSESIASFTEDQSHQYSTFKD
                     NYVVIQRILKSKFYPENLPAHALDSCESFKGYKKVRYLFDLCTDFLYKGNQLLQPLPT
                     VLPAGEVQEDVKIIVESLNALVIEQEPCAEALTETAPPVVVHVQENITQALQKELLQR
                     VSNFTETKQKSDTSSELRGINPALQFIAALSYEESATLLRWATKAPHL"
BASE COUNT         1510 a         1063 c          887 g         1561 t
ORIGIN      
        1 tttagcaatc gattgttgtc catacgtttt gtaaattcta acagcacgag cagtgccgat
       61 cccatgcgat tgtaaaaaga caacgatctc gcggatcatt ttttgatctt gccaggattt
      121 tacaacactg tctcgacgag aaatacctat accagacaat tccattaagc gatcaggggt
      181 gttttcaatg atatcaaaaa cctgcatagc aaatgcttgg attaactgtt tcgcaaagtg
      241 tggtccaata cccttcacta aacccgagcc taaatatttt tccataccct ctagtgttga
      301 aggcaaaaca atgcgtaaat gatccgcctt aaattgcaaa ccatactcac gattgttcac
      361 ccaagttcca ctacaatcaa tatattcacc tggacctacc gtgccagagc tgccaataac
      421 cgtgactaaa tctcgttgac ctttgacctt tacttttaaa acgcaaaaac ccgaatcttc
      481 attatgaaac gttactcgtt ctaccgaacc acttaaatgt tctacaggtg cttctgacat
      541 cattttccct tacggcggcg tacaatagtg tttttgcatt ttttgagcat ggatgagttg
      601 ttttttaagc gtattagatc gtctaatttt acagcctgta cgtaactccg tctctatctg
      661 ttttattttt tcactggttt tttcacaacg catcaaacga cgttcggcta aagccgcagc
      721 tttttcggct gttttttttt gacgcgcctc taatcgcatc tttttttctt gcagaagcag
      781 ctgtttttgc gttgcttttt gtgtgttatg cagctcccct tctttttcga gtacaatatt
      841 cggatcggtt ggcttataca cataaggcaa aaaatcatct tcccctgaag cgagttcaca
      901 gccccgatca cgaaattcag cgataccatt tttatcaaca caacgataga ctccggccaa
      961 taccatatca aacattaaaa ttccaaaaat taaaatcaaa aattttaaat gggaatactt
     1021 ttttcttgat accatcacgc caaccgccaa tcaatcggct cttttttcag gctttgtaaa
     1081 tactgatttg ttttagaaaa atgcttgcac cctaaaaaac cttgatgtgc tgaaaaaggt
     1141 gaaggatgcg tggtttttaa aacacaatgg cgatttgcat gaataaattg cccctttctt
     1201 tgtgcataag caccccacaa caaaaagaca atcccctcca ttgtttcgtt taggatttct
     1261 ataacccgat cggtaaattg ttcccatccg tgaccttgat gcgaagcagc gcgaccttgt
     1321 tcaacagtta atacactgtt taataataat acgccttgct tcgcccagtt gaccaaacaa
     1381 ccgtgttgcg ctggtggaat acctaaatcc gcttgcaatt ctttataaat attcactaaa
     1441 gaaggtggga gacgtacacc cggttgtacc gagaaacaaa ggccatgtgc ttgacctgga
     1501 ccatgatagg gatcttgtcc caaaataacc actttgactt gtgctaaagg tgtcaatgca
     1561 aaggcatgaa aaacatcact tgttttaggg taaataatct tcttttgatc tttttcctga
     1621 cgtaaaaaca aacgcaactg ccgcatatag ggcttatcaa actctgtgcc caaagcgtac
     1681 cgccaacttt cctctaacat agaacactct atataatcct tactcattcg tatattatca
     1741 caatcatgct taagttaact cctccaaaaa cagaagcaga actcttatcc cgctgctatc
     1801 aacttagcgg cataacgttt tctgcgttag ctcagcgtta ccaccaaaaa ttaccgtcct
     1861 ctcttacaca ggcaaaaggt ctgattggac agcttattga atacgcactc ggcgcaagtg
     1921 caggcaacct gcctgaaccc gattttccta atctaggcat tgaacttaaa acaattccta
     1981 ttgatgccaa tggccatcct cgggaaacga cttatgtctg taccgcacca atcaacttaa
     2041 aagcagaaat cgaatcttgg gaagattctc gagttcgaaa aaagctcgct cgcgttttat
     2101 gggtgcctgt tgaggcagac cccaagattc ctttacctga tcgctgtatt ggcaacgcac
     2161 agctttggag ccctgatcag cacaccgaaa ccaccttacg ccaagactgg agcgagctca
     2221 ctcacatgtt acacctgggt caaatagata atctatccgc taaaataggg acgtatttac
     2281 aaattcgacc caaagccgct catagtcgca ttttaaatcc gggtattaat gaacacgggg
     2341 aagaaatctt gataaatcct aagggttttt atttaagaac ctcgttaaca caaaaaatat
     2401 tacagtattg ttattgcaag gcttaagcac acaaaaaggc taaaaattag tttgaaaact
     2461 ttattatttt ttttatttac cctcgcaata ttggcaaaaa ccataaaagt cgtcatactg
     2521 ggttcttttt ttagttttaa gcaagtggaa ttacaatatg ccaatcgaaa caaatacaaa
     2581 aaacctaaag cctgctgctg gcaacgccag cgaaaccaag attgtgatta caacaggtct
     2641 tgccttattt gcgatgttct ttggtgcagg caatatccta tttcccctgt acttaggcgc
     2701 taacgcggga caaaacattg gcgtcatcac gctcggattt ttaattgccg gtgtcggcgt
     2761 acctttttta gggcttatcg caaccagttt atttaacgga agttatcacg ccttttttgc
     2821 tcgtttaggg aaaacgcccg gctttttgtt aattactttt ttgattatta ttataggccc
     2881 attaggcgca ataccgcgta cggaggtaac gacttttcat gccttgcaac cttatttgcc
     2941 acactttttg gataataatg ctatttttag ccttttttat tgtggtctcg tttttttact
     3001 ggcttaccga gaagccaaag tcgtagaaat tctgggttta tatttaagtc ccgttaaaat
     3061 tgtttcattt accgcattaa ttattttggg ttttatctct gtagaaccgg caatgactag
     3121 cccagccagt acatttcaag cttttaataa agccattatg aatggctata acaccatgga
     3181 tctcttaggc gccttttttt attgcgccgt tgcctttaaa tctataaaac aagcgataca
     3241 ccataatccg gcgctcaatc caaccaccct aaccttaagg tctagtatta ttggtgcggg
     3301 aattaccggt gctgtttata ttggatttat gtggattgcg cataatcatg ccgcaagctt
     3361 acaagggatc cccgaagaaa aaatgatcag cgctatttct atgctggtgc ttggaaagtt
     3421 tggtgcgata tttgtttgtg tatctgtctc ttttgcctgt gttgcaacag cgctcgcgct
     3481 ggctgacgta tgcactatct acctttataa cgaagtcttt cataaacgcg tttctaaaat
     3541 tgcctgcctt agcacggtga tgatcttaac gtatttgatg acgaacattg gctttcaggg
     3601 tattctgagt tttacagtgc ctattttgaa tatcgtctat cccgctttaa ttgttttgtg
     3661 catttgtaat attctgcata aatggaaagg cataaaaatg gtgaaactac ccgttttgct
     3721 aaccattctg atttttgtgg tgatttgtta gttattttta ttggttagtt ttaaatgttg
     3781 atcgcatgtt tgtgtgcgtg atagcattaa cttaattatt atagcggaga gacaaaaatg
     3841 cccggcaatt acacgtatta cgatccaaat caagtgctgt cgcaagcaca tatcgatgct
     3901 ttagcaaaaa gcaaaaaaat aatatcagcc cgaatgcttt acaataaagt tcagcttaaa
     3961 tttttgttag atccaaaaaa atcgaagcta gccagtgctc tctttaatat ttcataccct
     4021 caatttgaag gaacggaaca atttataaaa gcgacatttg tagaggttcg gaattataac
     4081 atatatattg aatatataga aacgtgtaga actgtagctg cctttgatga tttatatttt
     4141 cagtttttga ttgaatcaag atctcaacta tctaacgtag atagagtgaa taaacgtatc
     4201 ttaaaaagta aaagaattcc tggtatgaaa aaaagccaat cgatagaaga gtctgaaacc
     4261 ttcttcgagg ataatactga gctcaaatat tataaagaaa tttttgatat attaagaaaa
     4321 catatttcac aatgctctta tgaatttaca cagcacttta attttagtta cacaactatc
     4381 gaaaaaaatc ccattctaca aatcgactat tcccccttgg gaggagccct attcacaatt
     4441 gttaaaaaac tgaacaaacg tttgcgttca gaatcgatcg caagctttac tgaagatcaa
     4501 tcgcatcaat attctacatt taaagataac tatgttgtaa tccagcgtat acttaagtct
     4561 aaattttatc cagaaaatct tcctgcccat gcacttgatt cttgtgaatc ttttaaaggt
     4621 tataaaaaag tacgttatct tttcgatctc tgtacagatt tcttatataa aggaaatcaa
     4681 ctgctacagc ctttaccaac cgtactgcct gctggggaag ttcaagaaga tgtaaaaatt
     4741 atagttgaat ctttaaatgc acttgtcatc gaacaagagc catgtgccga agcattaact
     4801 gagactgctc caccagttgt tgttcatgtg caagaaaaca taacccaagc actacaaaaa
     4861 gaattattac aacgtgtatc gaattttaca gaaacgaagc aaaaaagcga cacgtcctca
     4921 gaattgaggg ggattaatcc cgcattacaa tttattgcag cgcttagtta tgaagaaagc
     4981 gcaacacttt tacgttgggc aacaaaagca cctcatctgg a
//
LOCUS       DAORVT010000039         5014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_11772_length_5014_cov_3.481347, whole genome shotgun sequence.
ACCESSION   DAORVT010000039 DAORVT010000000
VERSION     DAORVT010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11772_length_5014_cov_3.481347"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PK389_01340"
     CDS             <1..531
                     /locus_tag="PK389_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQY22390.1"
                     /translation="QQGVFYKVIDEYVYFYLKWIAPEKQALLGFDSSNEYFTEKIGTP
                     GYYNWMGYTFESICYKHIPQIRAALGISSGARVGTWRHIPPKGSKEQGVQIDLLFDRK
                     DDTVNICEIKCTEKPFVIDKLYYYSLLNKINVYKFETKTTKQLFVVFVSATGLKKNTY
                     ANEIVDRVVTLEDLFK"
     gene            complement(602..1486)
                     /locus_tag="PK389_01345"
     CDS             complement(602..1486)
                     /locus_tag="PK389_01345"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011270763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HQY22391.1"
                     /translation="MTQLDITLDLAGKLIREQFPEYAHLSITSVEKQGHDNRTYRLGN
                     DMLIRMPTAESYALKVPKEQALLPKLALHLTVNIPVPIKMGIPSKDYPYPFSIYQWLE
                     GKSIDLLVLDDDCQEKLAFDLAKFLKALQSIDDVDGPTPGQHNWWRGDPVGVYDKGTR
                     EQIAKLAGVIDADKAMNLWERACKTRWNKPPVWIHGDFAIGNMLLQDGRLSAIIDFGG
                     MALGDPACDLVIAWTFLKGKAREIFVKEIALDEDTWLRARGWALWKGTFELCQIEDKN
                     SPTSRLQKTIISNVLEES"
     gene            complement(1483..2571)
                     /locus_tag="PK389_01350"
     CDS             complement(1483..2571)
                     /locus_tag="PK389_01350"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY22392.1"
                     /translation="MKKMNIQFEKASARHIDTIFGWLSEVYVQAFWDNTQAHKDDILN
                     FIGGRTQPSSYADGKYVYWIASDNGYPFAMFMTIQATLEDHVNDIKLMYLSKTGHSYG
                     IEYMIGDQGYFDKGYGAKTLSEFLVFFRREFDPKADTFLIDPASDNPRAKHVYMKAGF
                     EHVADFIMSGEVSGAGKPHHLLIKKFALDVQLLPATKIIADQNIVIRLATSVDIEHMV
                     AMSYQKRRDYEKAQPQFWKWAGVDAEVLQAKWFSELIARDEHVILVATDSNEKIMGFI
                     IGRLMPAPAVYNPGGLTLMIDDYCIENEHLWGSVGIKLINEIKVIAKAKQAVQILVVC
                     GAHDKVKSQFLKNRGLAVASEWHVGSIV"
     gene            complement(2978..3493)
                     /locus_tag="PK389_01355"
     CDS             complement(2978..3493)
                     /locus_tag="PK389_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011477151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22393.1"
                     /translation="MKHGFIVCDNQLINNPIFTLLNYDGFSNIPEFGWNAIGRIRTVI
                     LDFMAMEHNHNYVLTNCLYEDEGDRNCYLQVEAMALKRDSIFIPVKLLISEEENLKRI
                     MEPSRRARWKSIDPQDVCQRKELLHVEHPHLLTLDVSALSAVQAVIEILEHVFNLKNS
                     NDGKYYITNRL"
     gene            complement(3568..4215)
                     /locus_tag="PK389_01360"
     CDS             complement(3568..4215)
                     /locus_tag="PK389_01360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012151742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HQY22394.1"
                     /translation="MTFFKSYRESIIIKDHIKARHIIVGEYSYYSGYYHDTSFEDCVM
                     YLDAADDHTGANEIDRLIIGKFCSIATGVKFMMGGTQGHNYEWIANYPLDFLDDDFDN
                     YTSVAPKGYKRKGDTIIGNDVWIGAEAMIMPGVQIKDGAVIGARSLVTKNVGAYEIWG
                     GNPVRLIKKRFKDDEIEKLLKIKWWGWELEKLKRHLPLIRSSDVNALWRAHQENA"
     gene            4463..>5014
                     /locus_tag="PK389_01365"
     CDS             4463..>5014
                     /locus_tag="PK389_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY22395.1"
                     /translation="MNYEKQFDVIVVGGGHAGTEAALAAARMGANTLLLTHNIATVGE
                     MSCNPAIGGIGKGHLVKEIDAMGGAMGRAADLGGIQFRILNQSKGPAVRATRAQADRV
                     LYKQAIRSIVENQPNLAFFQQAVDDIIVEGVDVKGVVTQGGICFFAKAVVLTTGTFLG
                     GKIHIGTRQHAAGRAGDPPSIALA"
BASE COUNT         1527 a         1029 c          904 g         1554 t
ORIGIN      
        1 cagcaaggtg ttttttataa agtgattgat gagtatgttt acttttatct gaaatggatt
       61 gctcccgaaa agcaagcttt attagggttt gatagtagca acgaatactt cactgaaaaa
      121 attggcaccc caggatatta taattggatg ggatatacat ttgaatctat ttgttataaa
      181 catatcccac aaattcgcgc tgcattaggt attagctctg gagcaagagt aggcacttgg
      241 cgtcacattc ccccaaaagg cagcaaagaa cagggagttc aaattgattt gttatttgac
      301 aggaaagatg acaccgttaa tatatgtgaa attaaatgca cagaaaagcc ttttgtaatt
      361 gataaactct actattattc tttattaaat aaaataaatg tctacaagtt tgaaactaaa
      421 acaacaaaac aactttttgt tgtctttgtt tctgcgacag ggcttaaaaa aaatacttat
      481 gccaatgaaa ttgttgacag agtggttact ttggaagatt tatttaaata aagatgaaaa
      541 gtttgatctc gtggttacat taatggattc tgtttcatat atctatacca acagtgattt
      601 tttaagattc ctctaaaaca tttgaaataa tcgttttttg taatctagaa gttgggctat
      661 ttttatcttc tatttggcaa agctcaaacg ttcctttcca caaagcccaa cctcgcgctc
      721 gaagccaagt atcttcatct agcgcaatct ctttgacgaa tatctcacgt gcttttcctt
      781 tgaggaaagt ccaagcgatc acaagatcac aggcaggatc acctaaggcc atgccgccaa
      841 aatcgataat ggcagataac ctgccatctt gcagtagcat attaccaatt gcaaaatcgc
      901 catgaatcca tacaggcggc ttattccatc tggttttgca agcgcgttcc catagattca
      961 tcgctttatc agcatcaata acacccgcta gttttgctat ttgttcacga gtacctttgt
     1021 cataaacgcc cacagggtca ccacgccacc aattgtgttg gcctggcgtt ggtccatcaa
     1081 catcgtctat gctttgtagc gcctttaaaa actttgctaa atcaaatgca agcttctctt
     1141 gacaatcatc atccaaaacg agaaggtcga tgcttttgcc ttcgagccat tgatatatcg
     1201 aaaatggata tggatagtcc ttcgaaggta tacccatttt tattggaaca ggaatattga
     1261 cagttaagtg aagcgcgagc ttcggtaata aggcttgctc tttaggcact ttcaaagcat
     1321 aagattccgc agttggcatg cggattaaca tatcgtttcc taaacgatat gtgcggttat
     1381 catggccttg tttttcaacc gaggtgatgg acagatgagc atactctgga aattgttccc
     1441 tgatgagttt acctgcaaga tcaagggtga tatcaagttg ggtcatacaa tcgatcccac
     1501 atgccattct gaagcaacag ctaatcccct attttttaga aactgacttt taaccttatc
     1561 gtgagcacca caaacaacga gaatttgtac agcttgttta gctttggcta taaccttaat
     1621 ttcatttatc agttttatgc caactgatcc ccataaatgt tcgttttcaa tacaataatc
     1681 atcaatcatg agtgttaatc cgccagggtt atagactgct ggtgctggca ttaacctgcc
     1741 aataataaaa cccatgattt tttcgttact atcagtggcg acgagaataa catgttcatc
     1801 acgtgcaatt aattcagaaa accatttggc ttgcaacact tctgcatcaa cgccagccca
     1861 tttccaaaat tgcggctgtg ctttttcata atcacggcgt ttttgataag acatagcgac
     1921 catatgctca atgtcaaccg aagtcgccaa gcgaattacg atgttttgat ctgcaataat
     1981 ttttgtggca ggcaatagct gaacatcgag cgcaaatttt ttaattaaca aatgatgcgg
     2041 tttacctgca ccagaaacct cgcctgacat tataaaatca gcaacatgct caaaacctgc
     2101 tttcatataa acatgcttgg ctcttgggtt atcgcttgct ggatcaatta aaaaggtgtc
     2161 cgctttagga tcaaactccc ttctaaaaaa aaccaaaaat tccgataacg ttttagcacc
     2221 ataaccttta tcaaaatacc cttgatcgcc aatcatatac tcaataccat acgaatgacc
     2281 tgttttagat aggtacatta atttaatatc gttaacatgg tcttccagtg ttgcttgaat
     2341 tgtcataaac atggcgaatg gatatccatt atcactggct atccaataaa catacttacc
     2401 atccgcataa gaagaaggct gcgtacgtcc accaataaaa tttaaaatat cgtccttatg
     2461 cgcctgagta ttatcccaaa acgcttgaac gtagacctca gaaagccaac cgaaaattgt
     2521 atcaatgtgg cgagcactcg ctttttcaaa ctgtatgttc atttttttca tatataaaat
     2581 catcaataaa agtcaccaaa tcatcatgat gaaaggctac tggaacattt tggtttttga
     2641 tcttttcttg gtaccaatga aatttcgcgt ggctcccacc taaaaataca acaactctca
     2701 taaaacgcca tcacaatcaa agattatcag ctttttcata tcattacact ctcaaatgaa
     2761 ttgttagaag gtgagttaat ccactgttaa accaattttc tcagcggctc tgctctaaaa
     2821 tctatttctg atattttatc taaaggcatc cacaacaatt cgatatgcgc ttcagtagat
     2881 ctaacgtttt acaaagcaaa atttggcctt gatcaataat cacgcctcga cttaaaacat
     2941 gaatattatt ttttgtcttt atcgtccgcc tcctgcacta tagtctgttg gtgatataat
     3001 attttccatc attgctattt tttagattga atacgtgttc taaaatttcg attactgctt
     3061 gaactgcaga caaggcactc acatctaatg tcagcaaatg cggatgctct acatgaagca
     3121 gctcttttct ttgacagaca tcttgaggat caattgattt ccatctcgct ctcctggaag
     3181 gctccataat acgttttaaa ttttcttctt ctgaaataag taatttcacc ggaataaaaa
     3241 ttgagtcacg ttttaacgcc atcgcttcta cttgtaaata acaatttcga tcaccttcat
     3301 cttcgtataa gcaattggtt aaaacgtaat tatgattgtg ttccatcgcc ataaaatcaa
     3361 gaatgacagt gcgaatgcgt cctattgcat tccaaccaaa ctcaggaatg ttactaaaac
     3421 catcataatt cagcaaggta aaaatgggat tattgatgag ttgattatca caaacaataa
     3481 aaccgtgttt catcaattct tagctaatgg tatattttcc agttcctgga tgaccaaata
     3541 gataaatact aatggtagta atattcatca cgcgttttcc tgatgcgctc tccaaagtgc
     3601 attaacatca gaggatctaa ttagcggtaa atgtcgcttt aacttttcta attcccaacc
     3661 ccaccattta atctttaaaa gtttttcgat ttcatcgtcc ttaaaccgct ttttgattaa
     3721 ccgcactggg tttccacccc aaatctcata agctcctaca tttttggtaa ctagactgcg
     3781 tgccccaata accgcaccat ctttgatttg aaccccgggc ataatcattg cttcagcgcc
     3841 aatccaaaca tcattaccaa taattgtatc gcctttacgt ttataacctt tgggtgctac
     3901 tgatgtgtag ttatcaaaat catcatccaa aaaatccagc ggatagttcg caatccattc
     3961 ataattatgg ccttgtgttc cacccatcat aaattttacg cccgtggcga tagaacagaa
     4021 tttaccgata atcagtcgat cgatttcatt tgcacctgtg tgatcatctg ctgcatcaag
     4081 atacatcaca caatcttcga aagatgtatc gtgataatag ccggaatagt aagagtactc
     4141 gccgacaatg atgtgccttg cttttatatg atctttaata ataattgact cgcgataact
     4201 tttgaaaaac gtcataggtt tatctcttta atgcgtgcat acataataga cattatgatt
     4261 gcacaaaata tcgttttatg tcaaaatata gttcaacaag aaggtaacag gtcaataaca
     4321 cgcaacatgt tgttttataa cctgtagctt ttcagataat tgttttggtg agcggatgca
     4381 tgtttgagcg aagcgagttc agcagcgaat caaaataatt atctggcaag ctctgttttt
     4441 gcagggacta ataggaaaat aaatgaatta cgaaaaacag tttgatgtaa ttgttgtggg
     4501 tggtggccat gcgggtaccg aagccgcatt ggctgcggcc agaatgggcg ctaataccct
     4561 attattaaca cataatattg caaccgttgg agaaatgtct tgtaaccccg ccattggagg
     4621 gattgggaaa ggacatttag tgaaagaaat cgatgcgatg ggtggcgcga tgggacgtgc
     4681 tgcagattta ggcgggattc aatttagaat cttaaatcaa agcaaaggac ctgcggtacg
     4741 tgcaacacgt gcacaagcag atcgggtttt atataaacaa gcgattcgat cgattgtcga
     4801 aaaccaaccc aacctcgcat tttttcaaca ggccgttgat gatattattg ttgagggtgt
     4861 tgacgtgaaa ggggttgtaa cacagggcgg catttgtttt tttgcgaaag cggttgtgtt
     4921 aacgaccggt acctttttgg gtggaaaaat tcatattggt actcggcaac atgctgccgg
     4981 gcgtgcaggc gaccctcctt ctattgcatt ggcc
//
LOCUS       DAORVT010000040         5011 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_11790_length_5011_cov_3.524415, whole genome shotgun sequence.
ACCESSION   DAORVT010000040 DAORVT010000000
VERSION     DAORVT010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11790_length_5011_cov_3.524415"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PK389_01370"
     CDS             <1..279
                     /locus_tag="PK389_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22396.1"
                     /translation="GIVDFSVEYGFFESSDTSLEGTSEKFQYYAAAEVKPEQWALVRM
                     VRLKIETKGETYHGISQKNHCWEYAFAIRNGHRVSSGIGVAISDIPYR"
     gene            287..1075
                     /locus_tag="PK389_01375"
     CDS             287..1075
                     /locus_tag="PK389_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22397.1"
                     /translation="MGQMILTAKTSASFQKQLFVGIEAENQLLEAEFKVKKNPERHLS
                     SAIGFVADHLEYGCQEGVQIFKVSVPPLESFVAVRMNRAQMPPLLNKADLLAKAGQPL
                     SLLAAVDLKQLGTVDRLYATDQNYIWSIDLTNRHNGQPYHQYRLAGLMEAKYLSNLHV
                     VPDAEGNGVCLYFLSEFTQKKGLYMIKDLLPIVNSGESLQHALSAVMLIAEGDYRAFF
                     VRFGRIVLIPTDPKCKPKALALPTHQHALVDDQLGRIAWRKIKK"
     gene            1385..2821
                     /locus_tag="PK389_01380"
     CDS             1385..2821
                     /locus_tag="PK389_01380"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HQY22398.1"
                     /translation="MKKRSTTLKKIGLTLAGLGAVMSTGAVAANNMQVKSEGGIMIYN
                     PSDSAYWFSVGGRLNLDETVFSGGRLDKTNQFASGGNIRRAFLKFAGGVGDYISYNLT
                     LDFSRPNLTQAGQFDGRGSNGHSVDFEDAYINACSEFTGAIGVANLRFGQFTPPTSID
                     DWGNHGTNNDTMFLESSLATSAFAIPTKVYGLWFDATAIDMFMLSASVFQPRQNHFNG
                     ASATNASTNGGLANNNNYGDTGRSDRLGAALRLTFAPVHTCDTVYHFGLMGRYQSMNN
                     SAQGNPIGQYNLFQTTPEARARQTGQLVTTGNVRGRSYNVVNGEALAMWGPATVEGEY
                     YQAIVQRIPTGGTALDVGTTRGNPRFHGWHIEAAYVLTGEHRVYDFQTGTLRNPKPCD
                     KMGAWEIAARYSFVNMVDKNIYGGSEHNTTLGLNWFVNEHVKLSANYIRANIYPNVSV
                     RSNNNVVTASSAAKRQLDIVGLRLAVAF"
     gene            2956..3684
                     /locus_tag="PK389_01385"
     CDS             2956..3684
                     /locus_tag="PK389_01385"
                     /inference="COORDINATES: protein motif:HMM:NF016236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HQY22399.1"
                     /translation="MSKNSCVKFFKLLGILLAVTHLIGCGKSPSVDRFEMLNRSTYSF
                     NKNVDRFLIKPISRAYEAIIPKPIQIIVGNFFQNLSEIPNIGNDILQGNFHYAQRDTA
                     RFLVNSTWGLGGLFDVAGKKGLERHHQDFGLTLAKWGYTESSYLVLPILGPSTIRDGV
                     GLGVTYQMGVSPHLKSVALRNNLMLLYYIDTRASLLKVEPLIGEAVDEYVFIRDAYLQ
                     RRKYLISGEKEETATEATLEGPPE"
     gene            3689..4885
                     /locus_tag="PK389_01390"
     CDS             3689..4885
                     /locus_tag="PK389_01390"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HQY22400.1"
                     /translation="MRTMMQLPLTANILVIDDDPILRKSIKDYFEDSGFTVIEAENGQ
                     VGLEIFKNQKPDLIVTDLQMPVLGGLEVLSAVTKASPETPIVVISEAGGLNDVIKALR
                     IGAWDYLTKPIVQIPVLEHAVCRALERSRLIEENRIYKRELELANQALKKSLDILEED
                     QEAGRSVQMKLLPEQGVIFGEYQFTHNVAPSLYLSGDFVDYFKISDKKFGFYIADVSG
                     HGSSSAFVTVLLKSAMGQILTRYKAGQDETILEPDKVLAKLGLEIHSAKLGKYLTMVC
                     GVVDLESHELTYSIGGHYPNPVLLENGKARFLEGKGFPVGIMKNAMYQRYTTHLNADM
                     RLLLFSDGIAEILPEKDMEAKEALLLSMVTEGKGSVAHFIEQLGLENKKNLPDDVTIL
                     SLVKGA"
BASE COUNT         1464 a          899 c         1169 g         1479 t
ORIGIN      
        1 ggtattgttg atttttcggt ggaatatggt ttttttgagt cgagtgatac gtcgcttgag
       61 ggcacctctg aaaaatttca atattatgcg gctgcggagg taaaacccga acaatgggcg
      121 ttggttcgca tggtgcgttt aaaaatagaa actaaaggtg aaacttatca tgggatctct
      181 caaaaaaatc actgttggga atatgccttt gcaatccgaa acgggcatcg tgttagttct
      241 ggtattggtg ttgctattag cgacatccct tatcgttagt cataagatgg gacaaatgat
      301 attaaccgct aaaacttcag caagttttca gaaacaactg tttgtcggga ttgaggctga
      361 gaatcaattg ttagaagccg aatttaaggt taaaaaaaat ccagagaggc atctgtcaag
      421 cgctatcgga tttgttgctg atcacctaga atatggctgt caggaagggg tgcagatttt
      481 caaagtgtct gtgccgccct tagaaagttt tgttgctgtt agaatgaata gggctcaaat
      541 gcctccgttg ttgaacaaag ccgatctgct cgccaaagct ggccagccgt tgtctttact
      601 ggcggctgtt gatttaaaac agttgggcac agtggatagg ctttatgcga cagatcaaaa
      661 ttatatatgg tctatcgatc tcacaaacag gcataatgga caaccctatc atcaatatcg
      721 attagcagga ctaatggaag caaagtacct aagcaatcta catgtggtgc cagatgcaga
      781 agggaatggt gtctgtttat attttttaag cgaattcact caaaagaaag ggctatatat
      841 gatcaaagat cttttgccca tcgtaaacag tggagagtct ttgcaacatg cattatctgc
      901 ggtcatgctg attgccgaag gggattatcg tgcctttttt gtgcgatttg gtcgaattgt
      961 gttgataccc acagatccaa agtgtaaacc aaaagcatta gctttgccta cccaccaaca
     1021 cgctcttgtt gacgaccaat tgggaagaat cgcttggcga aaaataaaaa aatgagaaaa
     1081 aaaacaatat aggtcatatt atgctgtgtt atttaaaaaa tgatttgcga agcttttgta
     1141 attagagctt gtttattcat aaaaattttg tttaaatcga tggtgtggac gttagcagaa
     1201 agctatagtt gacaagctgt tttaaagaca gtaagttaga gccgtaatgt cgcaattggc
     1261 atttatgcaa aagatctgct agcatctaag caatagcgga ctaatcacga ttgagtggat
     1321 tgaatacgta aattaaattg ggttcattag aaccttaacg cgtgtctagt taggagaaaa
     1381 gttcatgaag aagcgttcaa caacattgaa aaaaatcggg ttaacccttg caggcttagg
     1441 ggcagttatg tccacgggtg cagtagcggc aaataatatg caggtcaagt cagaaggcgg
     1501 tatcatgatc tacaatcctt cagacagtgc ttactggttt tcggtaggtg gtcgtttgaa
     1561 tctggatgag accgtatttt caggtggaag gcttgataaa acaaatcaat ttgccagtgg
     1621 cggcaatatt cgccgagcat tcttaaaatt tgcaggtggt gtgggcgatt atatctctta
     1681 taacttaacg ctggattttt cacgaccaaa cttaacacag gctggccagt tcgatggacg
     1741 tgggtcaaat ggtcattctg ttgattttga agatgcctat atcaatgctt gctctgaatt
     1801 tacaggcgct attggtgttg ccaaccttcg gtttggtcaa tttacaccgc ctacgagtat
     1861 tgatgactgg ggtaatcatg ggacaaataa cgatacgatg ttcttagagt cttctctagc
     1921 aaccagtgca tttgcgattc caacgaaagt atacggcctt tggtttgatg caactgctat
     1981 tgatatgttt atgttaagcg cgtctgtttt tcaaccaaga caaaaccatt ttaatggtgc
     2041 cagtgcaacg aatgctagca caaatggtgg gttggcgaac aacaacaatt atggggatac
     2101 aggtcgttct gatcgcctag gtgctgcgtt acgcttaact tttgcaccgg ttcacacctg
     2161 cgatacggtt taccacttcg gattaatggg tcgttatcaa tccatgaaca attctgctca
     2221 gggtaatccg attggacaat ataatttgtt tcaaacaaca ccagaagcaa gagcacgtca
     2281 aacaggacag cttgttacaa cgggcaatgt tcgaggcaga agttataatg ttgtgaatgg
     2341 tgaagctttg gctatgtggg gacctgcaac ggttgaaggt gaatattacc aagccattgt
     2401 tcagcgtatt ccaacaggtg gaacagcgct cgacgtaggc acaacacgag gcaacccacg
     2461 tttccatggt tggcatatag aggctgctta tgtcttaact ggcgagcatc gtgtatatga
     2521 ttttcaaacc ggaacgcttc gaaatcctaa accctgcgat aaaatgggtg catgggaaat
     2581 cgctgctcga tacagcttcg tgaatatggt agataaaaat atttacggtg gtagcgagca
     2641 taacacgaca cttggtttga attggtttgt taacgagcat gttaagcttt cggccaacta
     2701 cattcgtgca aacatttatc ctaatgttag cgtgcgctca aacaataacg ttgttactgc
     2761 tagtagtgca gccaaacgtc aattggatat cgttgggtta cgcttagcgg tagcattcta
     2821 attcgacggt ggggagcccc cgctacttca aaattaaaaa agccctgctg tttggtgggg
     2881 cttttttttg tattcaataa gtaaaaaagt agacaatgac tggtagaatt tttagattta
     2941 tcatacactg ttagtatgtc taaaaattcg tgtgtgaaat tttttaagtt gttggggatt
     3001 cttcttgccg ttacccattt aatcggatgc ggaaaatctc cttcggttga tcgctttgaa
     3061 atgttgaatc gttcaactta tagctttaat aaaaatgtag atcgcttttt gataaagccc
     3121 atctccagag cttacgaagc aattattcct aaacccatac agataatagt tggcaatttt
     3181 tttcaaaatc ttagtgaaat tcccaatatc ggtaacgata tccttcaagg aaacttccat
     3241 tatgcgcagc gcgatactgc gcgttttctg gtgaatagta cctgggggct aggcggatta
     3301 tttgacgttg caggcaagaa aggactagaa aggcaccatc aagattttgg tctcacatta
     3361 gcaaaatggg gttatacgga atctagttac ttggttttgc ccattttagg ccccagtacc
     3421 atcagagatg gtgttggctt aggggtaacg taccagatgg gggtttcacc tcacttgaaa
     3481 tccgttgctt tgcgtaacaa tttaatgctt ctctattata ttgatacgcg agcttcttta
     3541 ttaaaagtcg agcctttgat tggcgaggcc gttgatgaat atgtctttat tcgagacgca
     3601 tacctacaac gccgtaaata tctcatcagt ggcgaaaaag aagaaacagc aacagaggca
     3661 acattagagg gtcctcctga gtaaactaat gcgcacaatg atgcaactgc ctttaacagc
     3721 gaacatcctg gttatagatg acgatcctat cctgcgcaaa agtataaaag actattttga
     3781 agacagcggt tttaccgtta tagaagcaga aaatgggcaa gttggactcg agatttttaa
     3841 aaaccaaaaa cccgatttaa tcgttaccga cttacaaatg ccagtattag gcggattaga
     3901 ggttttgagc gctgttacta aagcatcgcc ggaaacaccc attgtcgtca tttctgaagc
     3961 gggtggttta aacgatgtga ttaaagcgtt aagaattggt gcatgggact atttaaccaa
     4021 accgatcgtg cagatacccg tgctagaaca tgcagtttgc agggcattag agcgttcgcg
     4081 gttaatcgaa gaaaatagaa tttataagcg ggaattggaa ctggctaatc aggcgctaaa
     4141 aaagagttta gatattttag aggaagatca agaagcaggt cgcagtgttc agatgaagtt
     4201 attgccagag caaggcgtta tatttggtga atatcagttt acgcataatg tggcgccttc
     4261 tttatatctt agtggcgact ttgtagacta ttttaaaatc agtgataaaa aatttgggtt
     4321 ttatattgca gatgtgtcgg gtcatggctc ttcttccgca ttcgtaacgg tcttattaaa
     4381 aagtgcgatg gggcaaatat taacgcgtta taaagcgggt caagatgaga caatattaga
     4441 accggacaaa gtactcgcaa aattgggttt agagattcac tctgccaagc taggtaaata
     4501 tttaaccatg gtttgtggtg tcgtggattt agaaagtcat gaattgactt atagtattgg
     4561 tggccattat ccaaatcccg tgttgttgga aaatggcaag gcgcgatttt tagagggaaa
     4621 aggattcccc gttggcatta tgaaaaatgc gatgtaccag cgttatacca cgcatttgaa
     4681 tgccgatatg cgtttactgt tgttttcgga tggcattgcc gaaatactgc cagaaaaaga
     4741 catggaagcg aaagaagctt tactgttgtc tatggtgact gagggcaagg gcagtgttgc
     4801 gcattttatc gaacagttag gtttagaaaa taaaaaaaac ttgccggatg atgtaacgat
     4861 actctcgtta gtaaaagggg catagctttt acaccaatgc catctcagac ttaataagtg
     4921 tattttttgt cttgggctgc actgtctttt ctttttcttc atggatccaa attttctttt
     4981 cgtcttctaa acgatcatct agaatcgctt g
//
LOCUS       DAORVT010000041         5010 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_11794_length_5010_cov_3.515641, whole genome shotgun sequence.
ACCESSION   DAORVT010000041 DAORVT010000000
VERSION     DAORVT010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11794_length_5010_cov_3.515641"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /gene="gmk"
                     /locus_tag="PK389_01395"
     CDS             <1..534
                     /gene="gmk"
                     /locus_tag="PK389_01395"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HQY22401.1"
                     /translation="LSDVSISVSYTTRKMRPQEQEGVDYHFVSLDKFKTMLKQGDFLE
                     HAKVYGHFYGTSRLSVFDVLRQGLDVILEIDWQGAKQVRTQFFETKSIFILPPSLEIL
                     EARLQKRHSDNALIVKERMNEAREQISHYKEFDYLVFNDKFEDALEDLKSIVRSQRVY
                     WRRSAALRAATITSLTS"
     gene            615..833
                     /gene="rpoZ"
                     /locus_tag="PK389_01400"
     CDS             615..833
                     /gene="rpoZ"
                     /locus_tag="PK389_01400"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007112267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HQY22402.1"
                     /translation="MARITIEDCLEKVGNRFKLVLIAARRARKLALGGTEPIVPWEND
                     KATVVALREVAAGLSTEMTVEEEKVESI"
     gene            820..2924
                     /locus_tag="PK389_01405"
                     /pseudo
     CDS             820..2924
                     /locus_tag="PK389_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495388.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
     gene            2943..4151
                     /locus_tag="PK389_01410"
     CDS             2943..4151
                     /locus_tag="PK389_01410"
                     /inference="COORDINATES: protein motif:HMM:NF005602.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HQY22403.1"
                     /translation="MKLDEYLSNLDAETEPMVERIMQWANINTSSFNVPGLERMAAVL
                     TQEFSVLECEGNAFSLPPIEHVDSMGISKRIDIGPALRFWKRPEAPVQVLLVGHMDTV
                     FELEHPFQVAIRKDERVLQGPGVTDMKGGICIMLEALKLFEKSPNASQLGWEVIINPD
                     EEIGSFGSGPLLEERAKAHHVGLLFEPAMDDQGTLAGDRKGCGNFVFIVHGRAAHAGR
                     AFDQGRNAIAMLAEVITQVDALNGQRDGVTLNVGHVQGGGATNVVPPLAICRLDVRIK
                     QTDDHAWVLDHLNAITAAANQKEGFKTELKGSFNRKPKALVGKTKALYDLLAEVGQEL
                     GDTISWKPSGGCCDGNNLSDAGLPNIDTLGVCGGNIHSDEEYLLVDSLVPRAKLTAAL
                     LLRLSEGRFN"
     gene            4186..>5010
                     /locus_tag="PK389_01415"
     CDS             4186..>5010
                     /locus_tag="PK389_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22404.1"
                     /translation="MCVKKILNALNIIVMIVFSSNIFAIEWRNKIQILKTSPVKSTIF
                     MRDFLISKGQKANFDNKVTLITLEDGTKAVFKPVLQHDLGDAHAEVAAFKAAQFLGFP
                     EIPPTIIRKIKNEIGSLQLYVEPTVDSLQPGVYEETLKKVSPEDLANLKLFYFVFGQW
                     DTGPQNLIIRKELDGIHFVAIDNSGIKNRQYVHYGELPFVRICYSDVLDTKDWHLPFP
                     YEKVKILNNTRFEKLSEIFGDKFPKKILKNLSKYTAPLPYAIYKNSLWIQFHKADPA"
BASE COUNT         1558 a          830 c         1162 g         1460 t
ORIGIN      
        1 ctttcggatg tctctatttc tgtttcgtac acaacccgaa aaatgcgacc tcaagaacaa
       61 gagggagtag attatcattt tgtgagtttg gataaattta agaccatgtt aaaacaggga
      121 gactttttag agcacgcgaa agtttatggc catttttatg ggacatcccg attgtcggta
      181 ttcgatgtgc ttcgtcaagg gctagacgtt attttagaaa ttgattggca gggtgcaaag
      241 caggttcgta cacaattttt tgaaaccaaa agcatcttta ttttgccacc ctcgcttgaa
      301 attctagaag cgcgtttaca aaagcggcat tcagataatg cactgattgt taaagaacgt
      361 atgaatgagg caagagaaca aatatcccat tataaagaat ttgattactt ggtttttaat
      421 gataaatttg aggatgcttt agaggactta aagtcaattg ttcgatcgca acgagtgtac
      481 tggcgtcgga gtgctgccct tcgagctgct acaataacct cattaactag ttagataaat
      541 tatactgagg ttgtcgtata aaaaagggaa tatggtgtat gatggtgatc tattaaattg
      601 attgaggttt taaaatggca cgtattacga tagaagactg tttagagaaa gtgggcaata
      661 gattcaaatt ggttttaata gccgctaggc gtgcaagaaa attggcttta ggcggcacag
      721 agcctattgt gccctgggaa aatgataaag ctaccgttgt cgcgctccgg gaagtggccg
      781 caggactttc tactgagatg acggtcgagg aagagaaggt tgaatcaatt tgatgaactt
      841 tgtcaaaaac tgaaaggcta tttagcagaa gaccaagttg tagaaatcag caaagcctat
      901 ctagtctctg caaaagcgca tgagggacaa caacgctatt cgggtgagcc ctatatttcg
      961 catccggttg ctgttgcaag aatccttgcc gacatgaaca tggatacaaa aagcattatt
     1021 gcagcgatat tgcacgatgt cttagaagat accctcatcg ataaagcgac tattatcgaa
     1081 caatttggtg aagaggtggc agagctcgta gacggggtta gcaaacttga acaaattaat
     1141 tttgagagtc gcgtagaagc acaagccgaa aatttacgta aaatgatgtt ggctatggca
     1201 cgtgatatcc gtgttatctt gataaagctc gctgatcgtt tacataatat gagaacccta
     1261 gaagcaatgc cactcgataa gcgtcggcgc attgctaaag aaacactcga aatttacgcg
     1321 cctatcgcga atcgtttggg tatgaataat tttcgaattg aatttgaaga tatcggtttt
     1381 tcatttttat atcctgttcg gtatcgcgtt cttaaagatg ctgttcgaaa agcgagaggg
     1441 aatcgcaaag aactcattca agatttagaa ttcaatatca aaaatagatt aacacaagaa
     1501 aatatcgccc cttttgttat catggggcga gagaaacatt tgtacagtct ttataagaaa
     1561 atgcgaaaaa aagatctttc gttgtcagag atcatggatg tctatgccat taggatctta
     1621 gttgagaagg tagatgattg ctatcgtgtt ttgggtgttg ttcataatac ctataggcct
     1681 gtgcatggca gatttaaaga ttatattgct attcctaagg ccaatggtta ccaatcactt
     1741 catacaaccg tattagggcc ctatggcgtg cctatagaaa tacaaattcg cacaaccgac
     1801 atggatcgta tggcagaaaa tggaattgct gcgcattggt tatataaatc tacagaaggt
     1861 gttgcgggaa cagcagaaat gcgtgcgaga gaatggctca aaggcatttt ggaaattcag
     1921 aaaaatactc gaaattccat ggaatttatt gaacatgtta aagtcgatct ttatccggac
     1981 gaggtttacg ttttcactcc tggtggagat atcctttcgt tacccagtgg tgctaccgcc
     2041 gtagattttg cctatgctgt tcatacagat gttggaaatg cctgtattgc ggttaaaata
     2101 gacagacggt tagtaccctt aagttcaaga attaatagtg gtcagacggt agaaattatt
     2161 acagcgatgg gtgcacatcc taatcccgct tggctaagtt ttgtcgctac cggaaaagcg
     2221 cgtagtaaca ttcgccattg gttaaaaacg caacaagtga gtgaggccag aatactgggc
     2281 aagcgcctaa ttgaacgcgc attggtttcg ttatcgctgt cgttagaaga agtttacgaa
     2341 aaccggatta agacagtttt gcaagaatta agtttagaaa actttgatca gttatgtgaa
     2401 gaagtgggtt taggtaatca gcttgcaccg ttagttgtaa ggcgtttagt gtctgatttg
     2461 cccgtggaag agtctcatct tgtttatccg ttagcgattc gtggcacaga agggatgggg
     2521 tttcttatgc aaaatgttgc agacccattc ctggagatat tattgcagga tttttaagtg
     2581 cagggcgagg aattgtaata cacagagaag gatgcaaaaa tcttgccgaa aaacagaagt
     2641 cgcctgaaaa atatattttt attcaatggt cagataaggt gataggcgaa tttcaaattg
     2701 aattgcgtat tgaagtttta agtgagcgag gcgttttagc aatattagca aataaattat
     2761 ccgagaatga ggccaacatt caaaatgtac atattgatga aagagatagc cgtcatcata
     2821 ccattacatt tttgttaagt attcaaaatc gcaatcattt agcaaagtta attcgacaat
     2881 tgagacaaat agaaattgta acacatgttg ttcgagtgaa ataaaaaaag ggggtattca
     2941 gcatgaaatt agacgaatat ttatcgaatt tagatgctga aacagaaccg atggtagagc
     3001 gaattatgca atgggcaaat atcaatacga gttcttttaa tgtgccagga ttagaacgta
     3061 tggcagctgt attaacacaa gaattttctg tattggaatg cgaaggtaat gcgttttcac
     3121 taccgcccat agagcatgtg gattccatgg gtatcagcaa gcgtattgat ataggacctg
     3181 cactacgatt ttggaagcgg ccagaagcac ccgttcaagt gttgttggtg ggacatatgg
     3241 ataccgtatt tgagttagaa catccttttc aggttgcgat ccgaaaagat gaacgtgttt
     3301 tgcaagggcc gggcgtgaca gatatgaaag gcggcatttg tattatgtta gaggctttaa
     3361 aattgtttga aaaaagccca aatgcgagtc aattgggatg ggaagtcatt atcaatcccg
     3421 atgaagaaat tgggtccttt ggatcaggtc cacttttaga agagcgcgcg aaggctcatc
     3481 atgtaggatt gctttttgag ccagcgatgg atgatcaagg tactttagcc ggcgatcgca
     3541 aagggtgtgg taattttgtt tttattgtgc atgggcgcgc tgcacatgca ggacgcgctt
     3601 ttgatcaagg tcgcaatgcg attgcgatgt tggccgaggt tattacacaa gtcgatgcgt
     3661 taaatggtca gcgcgatggt gtaacgctta atgttggtca cgtacaaggg ggaggcgcta
     3721 ccaatgttgt tccaccattg gcaatttgtc gtttagatgt tcggatcaaa caaaccgacg
     3781 atcatgcttg ggtattagat catttaaatg cgattacagc cgctgcaaat caaaaagaag
     3841 gctttaaaac agaattgaaa ggcagtttta atcgaaaacc aaaagcgctt gtaggcaaaa
     3901 caaaagcact ctatgactta ctcgcagaag ttggccaaga attgggtgat accatttctt
     3961 ggaaacccag tggcgggtgt tgcgatggca ataatttatc ggatgcaggc ttacctaata
     4021 ttgatacctt gggtgtttgt gggggtaata ttcatagcga tgaggagtat ttattggtgg
     4081 atagcttggt gccaagagca aaactaactg cagcgttgtt gttgcgctta agtgagggaa
     4141 gatttaacta acatagagcg ctcttaaata aaaaagaaaa caagtatgtg tgtaaaaaaa
     4201 atattgaatg ctttaaatat tattgtgatg attgtttttt catcaaatat ttttgccatt
     4261 gaatggagaa acaagattca aatattaaag acatcgcctg ttaaaagtac tattttcatg
     4321 cgcgattttt taatttcaaa gggacaaaaa gcaaattttg ataataaagt cacgctcatt
     4381 actttggaag atggaactaa agcggtgttt aaacctgttc tccagcatga tttaggagat
     4441 gcacatgcag aggttgcggc ttttaaggct gcgcagtttc ttggttttcc ggaaattcca
     4501 cccacaatca ttcgaaaaat taagaatgaa attggatcct tacagcttta tgttgaaccc
     4561 acggtcgata gtcttcagcc gggggtatat gaagaaactt taaaaaaagt ttcaccagaa
     4621 gacttagcca atcttaagtt gttttatttt gtttttggcc aatgggatac gggcccacaa
     4681 aaccttatca tcaggaaaga attggatggt attcattttg tagccattga taacagcggg
     4741 attaaaaacc gccagtatgt tcactatggt gaacttcctt ttgtaagaat ttgttatagt
     4801 gatgttctgg atacaaaaga ctggcatttg ccttttccgt atgaaaaagt gaaaatcctc
     4861 aataacacac gttttgaaaa gctttcagaa atttttggcg ataaatttcc aaagaaaata
     4921 cttaaaaatt tatcaaagta tacggctcca ctgccgtatg ctatttacaa aaatagtctt
     4981 tggatacaat tccataaggc tgatcccgct
//
LOCUS       DAORVT010000042         4988 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_11893_length_4988_cov_3.215488, whole genome shotgun sequence.
ACCESSION   DAORVT010000042 DAORVT010000000
VERSION     DAORVT010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11893_length_4988_cov_3.215488"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..1689
                     /gene="atpA"
                     /locus_tag="PK389_01420"
     CDS             148..1689
                     /gene="atpA"
                     /locus_tag="PK389_01420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HQY22405.1"
                     /translation="MILNPSEISELIKENIKNYNVAPIVKNVGKIVSLADGIVRIHGL
                     ETVQYGERLSFEGNHFGIALNLERDSVGAVVLGSTEDLAEGQKVWCTGEILKVPVGEA
                     LLGRVVDALGRPIDDRGAIEASEMSPIEKVAPGVIWRQSVSQPLQTGLKSIDTMVPIG
                     RGQRELIIGDRQTGKTAIAIDTIINQKNSGIKCIYVAIGQKASQVASVVRKLEEHGAM
                     KHTIVVAATAAESAALQYIAPYSGCTMGEYFRDKGEDALIIYDDLTKQAWAYRQVSLL
                     LRRPPGREAYPGDVFYLHSRLLERASRVNEAYVERATEGRVKGRTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIYLETDLFNAGVRPAINAGLSVSRVGGAAQTKIIKKLGGG
                     VRLALAQYRELAAFAQFASDLDEVTRKQLERGQRVVELLKQKQFSPLTVAEMAVSLIS
                     SEKGYLDDISLKKIVDFERDLLQHVRLHHMALLQRINQEQTLSPEIELALISVVEEFK
                     AAWII"
     gene            1689..2558
                     /gene="atpG"
                     /locus_tag="PK389_01425"
     CDS             1689..2558
                     /gene="atpG"
                     /locus_tag="PK389_01425"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005893190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HQY22406.1"
                     /translation="MLDAKEIRTKVSSIKNTQKITRAMQMVAASKMRKAQDRMHASRP
                     YAEKVKVVISHLAQGRLEYKHPYLIEREPKRVGFMVVSTDRGLCGGLNANLLKVTVQD
                     MRTWHEKETPIDICSIGKKAESFFKRMGGNIVSSVSNLGNKPSILDVIGTVKVMLDLY
                     NEEKLDRIYLVYNKFINTVIQKPMLEMVLPIVDTTPSIENNGKFSWDYLYEPDAKGLL
                     NALLVRYIESLVYQGIVENIACEQAARMIAMKNASDNASELIDELQLMYNKARQASIT
                     RELSEIVAGAAAV"
     gene            2568..3950
                     /gene="atpD"
                     /locus_tag="PK389_01430"
     CDS             2568..3950
                     /gene="atpD"
                     /locus_tag="PK389_01430"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HQY22407.1"
                     /translation="MDTGKIVQIMGAVVDVEFSKGLIPKIYDALRVEEADLTLEVQQQ
                     IGDGVVRTIAMGPSEGLCRGLSVTNTGAPITVSVGKKTLGRIMDVLGRPIDEKGPIGS
                     ETFLPIHRSPPAYSEQAANEALLETGIKVIDLICPFAKGGKVGLFGGAGVGKTVNMME
                     LIRNIAIEHSGYSVFAGVGERTREGNDFYHEMKESGVLDKVSLVYGQMNEPPGNRLRV
                     ALTGLTIAEQFRDEGRDVLLFIDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEMGV
                     LQERITSTKKGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRQIAELGIYPAID
                     PLDSTSRQLDPNVIGEEHYDVARGVQGTLQRYKELKDIIAILGMDELSDEDKRMVARA
                     RKIQKCLSQPFFVAEVFTGSSGKYVSLKETIRGFAGIVAGEYDHVPEQAFYMVGTIDE
                     VLEKAKTLTV"
     gene            3963..4388
                     /locus_tag="PK389_01435"
     CDS             3963..4388
                     /locus_tag="PK389_01435"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HQY22408.1"
                     /translation="MNISFHLDIVSAEAAIFSGLVEKLFVSGEMGELEILFNHAPLLT
                     SLKPGIVWVVKQNGQEEVFYISGGMLEVQPKATTILADTAIRAKDVDEAEALEAKKRA
                     EQILSGHTKEFDYVQAQSQLLEAVAQLKALKKFREKHRS"
     gene            4561..4914
                     /locus_tag="PK389_01440"
     CDS             4561..4914
                     /locus_tag="PK389_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017292543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HQY22409.1"
                     /translation="MKKEIRFYQTNSGKRPFIDWLEAQKDKVVRAQVNARLARLAVGN
                     YGDTKSIGKGIQEARIHYGAGYRMYFAELDNEIILLLLGGSKNTQEKDIKKAKEYWQK
                     FMEGIELCEKNTLKV"
BASE COUNT         1520 a          852 c         1210 g         1406 t
ORIGIN      
        1 gaaaaatatt ttgcgcgaaa ggtgaccatg gcgtgttcta tcaatacgag tctcttaggc
       61 ggctttttag ctaaggccgg caattttgag atagatggtt ctattcgtgg atctttaaca
      121 aatcttaaaa tggctatggg tgattaaatg attctaaatc cgtccgaaat cagtgaatta
      181 attaaagaga atattaaaaa ttataatgtg gcacctattg ttaaaaatgt cggtaaaatt
      241 gtgagtcttg ccgacggtat tgtgcgtatc catggtttag aaactgtaca atatggggaa
      301 cgcttatcat ttgaaggaaa tcattttggg attgcgctta atttagagcg tgattcggtc
      361 ggtgctgtcg tgttgggttc tacagaagat ttagcagaag gtcaaaaggt ttggtgtaca
      421 ggcgaaattt taaaagtccc tgtaggagaa gcgttattag gacgcgtggt agatgcttta
      481 gggcgaccca ttgatgatcg cggtgcgatt gaggcgagcg agatgtcgcc catagaaaaa
      541 gttgctcccg gcgttatttg gcgacaatcg gttagccaac ccttacaaac aggtcttaaa
      601 tcaatcgaca ccatggtgcc tattggtaga ggacagcggg aattaattat tggtgataga
      661 caaactggaa agacggccat tgccattgat accatcatta atcaaaaaaa ttctggtata
      721 aaatgtattt atgttgccat tggtcaaaag gcatcgcagg ttgcatcggt ggttcgaaaa
      781 ctagaagaac atggcgccat gaagcatacc attgtggttg cagcaacagc cgccgaatct
      841 gctgcgctgc aatatattgc gccctattcg ggttgtacca tgggcgaata ttttagagat
      901 aaaggtgaag acgcgttaat tatttatgat gatttaacca agcaagcatg ggcctatcgt
      961 caagtttctt tgttattacg tagaccgccg ggtcgagaag cgtatcccgg tgatgtattt
     1021 tatttgcatt cgcgtttatt agaacgggcg tcacgcgtga atgaagccta tgttgagcgt
     1081 gcaacagaag gtcgtgtaaa aggacgtaca ggttcgctga ctgcgttacc gattatcgaa
     1141 acacaagctg gggatgtttc cgcttttgtg cctacgaatg ttatttcaat tacggatggg
     1201 cagatttact tagagacaga tttatttaat gcgggcgttc ggcctgcgat taatgcaggg
     1261 ctttctgtct cgcgtgtggg tggtgcagct caaactaaga tcattaaaaa attgggtggt
     1321 ggcgtaaggc ttgcgctggc gcaatatcga gagttggctg ctttcgcaca atttgcttcc
     1381 gatttagatg aagtcacacg taaacagtta gagcgaggac aacgcgttgt tgagctatta
     1441 aaacaaaaac aattttcacc gctgactgtt gcagaaatgg cggtctcttt aatatcctca
     1501 gaaaaaggct atttagacga tatttcttta aagaaaatcg tcgattttga gcgtgactta
     1561 ttgcaacatg tacgcctgca tcatatggca cttttacaac gaatcaatca ggaacaaaca
     1621 ttaagtcctg agattgaatt ggccttaata agcgtggtcg aagaatttaa agcggcctgg
     1681 attatataat gttagacgca aaagagatcc gcacaaaagt tagcagtatt aaaaatacgc
     1741 agaaaattac gcgggcaatg caaatggttg cggccagtaa aatgcgaaaa gcacaagatc
     1801 gcatgcatgc gtcacgacca tatgcagaaa aagtcaaagt tgttattagt caccttgcgc
     1861 aaggtcgatt ggaatataaa cacccctatt taatcgaacg agaacccaaa cgggttggtt
     1921 ttatggtcgt ctcaaccgat agagggcttt gcggaggctt gaatgctaat ttattaaaag
     1981 tgacggtgca agatatgcgc acttggcacg aaaaagaaac acccattgat atttgtagta
     2041 ttggtaaaaa agctgagagt ttttttaagc gcatgggagg caatattgtc tcctctgtct
     2101 caaatctcgg aaataagccc agtattttag atgttattgg tacagtaaaa gtgatgcttg
     2161 acttgtataa cgaagagaaa ttagatcgca tttacttggt ttacaataaa tttatcaata
     2221 cagtgattca aaagcccatg ttagagatgg tgttacctat cgttgatacg acacccagta
     2281 ttgaaaacaa cggaaaattt tcttgggatt atttatacga gcccgatgcc aaaggtttgc
     2341 ttaatgcttt gttggtgcgt tatatagaat cattagtcta tcagggtatc gtcgaaaata
     2401 ttgcttgtga gcaagcagcc agaatgattg cgatgaaaaa tgcatcggat aatgcttccg
     2461 aattaattga tgaacttcaa cttatgtata ataaagccag gcaagcttca attacccgag
     2521 aattatcgga aattgtagca ggtgctgcag cggtttaggg ggaataggtg gatacgggaa
     2581 aaattgtaca aattatgggt gctgtcgttg atgtagaatt ttctaaaggt ttaattccta
     2641 aaatttatga cgccttaagg gttgaagaag cagatctgac tttagaggta caacaacaga
     2701 ttggcgatgg tgttgttaga acaattgcga tgggtccttc cgaagggttg tgtcgtggtt
     2761 taagcgtaac gaatacgggg gcgccaatta cagtttcggt cggtaaaaaa accttgggtc
     2821 gaatcatgga tgtcttagga cgcccaattg atgaaaaagg gccgattggt tcagaaacat
     2881 ttttacctat tcaccgttcg ccacccgcct atagtgaaca agcggcaaat gaagcgttac
     2941 tcgaaacggg tatcaaagtg attgatctga tttgtccttt tgctaaaggt ggaaaagtcg
     3001 gattgtttgg tggtgcaggt gttggcaaaa ccgttaacat gatggagttg attcgaaata
     3061 ttgcgataga acatagcgga tattctgttt ttgcaggtgt gggagaacgt acgcgcgaag
     3121 gtaatgattt ttatcacgaa atgaaagaat caggtgtttt agataaagtg agtctggttt
     3181 atggtcaaat gaatgagcct ccgggcaatc gcttacgggt tgcgctaaca ggcttaacca
     3241 ttgcagaaca atttagagac gaaggccgtg atgtgctgtt gtttatcgat aatatttatc
     3301 gctatacctt ggcaggaacc gaagtttctg ccttattggg tcgaatgcct tccgccgtag
     3361 gatatcaacc cacactggcg gaagaaatgg gtgtattaca agaacgtatt acttctacga
     3421 aaaaaggctc tattacttcc attcaagcgg tttatgtgcc ggcagacgat ttaaccgatc
     3481 cttctcctgc aacaaccttt gcgcatttag atgcgacagt tgtgttgtct agacaaattg
     3541 cagagctggg catttatccg gctatcgatc cgctagattc aacgagcaga caattagatc
     3601 ccaatgtgat tggtgaagaa cattacgatg ttgccagagg ggtgcaaggt acgctgcagc
     3661 gttataaaga actcaaagat attattgcca ttttgggtat ggatgaactt tcagacgaag
     3721 ataaacgtat ggttgcacgc gcacgcaaga tccaaaaatg tctgtcgcag cctttcttcg
     3781 ttgcagaagt atttacaggt tcttctggaa aatatgtctc tctgaaagaa actattcggg
     3841 gttttgcggg tattgtagcc ggcgaatacg atcatgttcc cgaacaggca ttctatatgg
     3901 ttgggacaat tgatgaagtt ttggaaaaag caaaaacatt aacggtgtaa cagcgaaatt
     3961 ttatgaatat tagttttcat ttagatattg ttagtgctga agcagcgatt ttctcaggtc
     4021 tcgtggaaaa gctctttgtg agtggcgaga tgggcgaact cgaaatttta tttaatcatg
     4081 cacccttgtt aacttcgtta aagccaggca ttgtgtgggt ggtaaagcaa aatggtcaag
     4141 aagaagtttt ttatatttcc ggtggaatgc tagaagtaca gcccaaagcc acaactattt
     4201 tagcagacac ggccattcgc gccaaagatg tagacgaggc agaagcgtta gaagcaaaaa
     4261 aacgcgccga gcaaattttg tctgggcata ctaaagaatt tgattatgtg caggcgcaaa
     4321 gccaattgct cgaagcggtc gcgcaattaa aagcactcaa gaaattccga gaaaagcatc
     4381 ggagctagaa gactttagcc ccacaataca cctacctttt gtttatctta tagaccagtt
     4441 agctgtaaat aaaatcagtt tgtttttgta taaaataagt gtagcctaca agctacattt
     4501 tgtatgcaag gaggcttatg aaaaggtttt taacccaatt cggatgattc ggagtgttct
     4561 atgaaaaaag aaataagatt ttatcaaacc aacagcggaa aacgtccatt tattgactgg
     4621 ctcgaagctc agaaagataa agtagtacgc gctcaagtaa atgctcgttt agcaaggttg
     4681 gcggtaggaa attatggaga caccaaatca attggaaagg gtatacagga agcaagaatt
     4741 cattacggtg ccgggtatag gatgtatttt gctgaattgg ataatgaaat tatactattg
     4801 ttactaggtg gttcaaagaa cactcaagaa aaagatatca agaaagcaaa agagtattgg
     4861 caaaaattca tggaggggat cgaattatgc gaaaaaaaca cattaaaagt ctgagtggca
     4921 attacgaaga atggctgatt gaatctttac gtgataaaga agaggctgct atctttttac
     4981 aagttgct
//
LOCUS       DAORVT010000043         4974 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_11961_length_4974_cov_4.973572, whole genome shotgun sequence.
ACCESSION   DAORVT010000043 DAORVT010000000
VERSION     DAORVT010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11961_length_4974_cov_4.973572"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PK389_01445"
     CDS             <1..235
                     /locus_tag="PK389_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HQY22410.1"
                     /translation="FEAKQAITRSFPLEISNNQKITDVVARMGFYQLPLDSLTTYQKQ
                     VEAVTISEIKSAFQRRINPEKLGLIIVGTATSP"
     gene            235..819
                     /gene="rsmD"
                     /locus_tag="PK389_01450"
     CDS             235..819
                     /gene="rsmD"
                     /locus_tag="PK389_01450"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HQY22411.1"
                     /translation="MLNEVRIIAGKWRGRKIQFPTVAGLRPTPNRIRETLFNWLAPYI
                     HGAHCLDLFTGSGALGFESLSRGAAHVLCLEKDRDAYEMLRQNAKRLNADLMEIQEQD
                     ALVWITTQCQQALKLTTPFDIIFVDPPYALKALSKCFALLENQAWLTINTLIYFESDV
                     PIELLTLPDTWAILKKKKAGSVYYYLTQKIKDQA"
     gene            complement(838..1212)
                     /locus_tag="PK389_01455"
     CDS             complement(838..1212)
                     /locus_tag="PK389_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22412.1"
                     /translation="MRVVTQNRELPEAIIPVPLHIDRLRKRGFNQALELLWPIHKKTK
                     IPVVFDACVRIRKTTAQAQLDKTRRKRNLKSAFQIVKPIPFEHVAIMDDVVTTGSTVN
                     ALTQVLKEAGVTTVDIWCICRA"
     gene            1429..2865
                     /locus_tag="PK389_01460"
     CDS             1429..2865
                     /locus_tag="PK389_01460"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HQY22413.1"
                     /translation="MGTINQQSVREEAERIKTEFDRLSVDKKINSETKMLIRSMFMLI
                     NLLIAIFLEKTTKKNNKNSSKPSSQTEKDESSLTNHGTNSKGKPESNTTADNTRTVES
                     ITIAEVTQCDVCGQDLSNIAHQHVERRTKIDIIFEKVIEHIDAHVKLCPDCGSTVKGV
                     FPANMHGPLQYGNGLKAYVINLLICQMISLNRVQKLIKSMIGEILSEATLLKFILRLH
                     LALAQWEADSIELLLISPAMNVDETSLRVDKKNHWIHVYSAGDVTLKLLHPRRGTEAI
                     NDINIIPRYSGTIIHDCWSSYLTYNDCDHGLCGSHLVRELTCIIESNGYNWAVNMKEL
                     LLETCKIVSKRKRKKLSEGEYANLQKRYRNIITRGEKELPAIPPKPNGKRGKMAKSDA
                     HNLWERLRDYEYSVLLFAKKSEVSFTNNRAERDLRMSKVKQKVSGCFRKELYAQAYCR
                     ISSYLQTMATKGYNPLIAIQMALVGESI"
     gene            3243..4118
                     /gene="bioC"
                     /locus_tag="PK389_01465"
     CDS             3243..4118
                     /gene="bioC"
                     /locus_tag="PK389_01465"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: protein motif:HMM:TIGR02072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HQY22414.1"
                     /translation="MHLSQNAHKPKLALARHFSRAAHTYNQAAIIHQEVGMRLLDRLD
                     LINLDPDVILDLGSGTGFFASHLTQKYPKAHIIKLDIAENMLRFSNKKHFAKHTSVCG
                     DGDYLPLKNHSIDFVFSNCVLHWFVNPQKVLAEIRRVLKPEGLLLFSTFGPDTLQELR
                     ESFSIIDQNTHVNAFMDMHHIGDMLIQTQVLDPVMDMEMITLTYQNLMHLIQDLKDTG
                     ENYVKREKSIGLSPKGTVDKLINAYEHYRTREGFLPATFEVIYGHAWCSGSTTLHQAD
                     EEGIIHFPIADLQIL"
     gene            complement(4130..>4974)
                     /locus_tag="PK389_01470"
     CDS             complement(4130..>4974)
                     /locus_tag="PK389_01470"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HQY22415.1"
                     /translation="FYTDLYARQNKRGGAWILGCKTRMQYPDGQRQLPAVYLVANFSS
                     TQTGGSVLLTHEEVITLFHEFGHCLHYLLTTVDYPSISGGNGVSWDAIELPSQLLEYW
                     CWEEEALALFSEHIQTKDPLPHDLFLKLKATKNFQIGLRLMRQLEFSLFDFVLHKTFS
                     ADLSKNMDIQKILDHVRSKTAVMPVPKFNHYQNTFSHIFGGGYAAGYYSYLWSEVLAA
                     DVFEQFTADNQVFNKDVSKKFVETILSKGGAEECMDLFVAFCGRAPKIDALLKEYDID
                     RIFE"
BASE COUNT         1592 a         1041 c          921 g         1420 t
ORIGIN      
        1 cttcgaagcc aaacaagcca ttacccgttc ttttccgcta gaaattagca acaatcaaaa
       61 aataaccgat gtcgtagccc gaatgggttt ttatcagctg ccgctcgatt cgctgacaac
      121 gtatcaaaaa caagtcgagg ctgtcaccat ctccgaaata aaatccgctt ttcagcgccg
      181 tattaaccca gaaaaattgg gattaattat tgtaggcacc gcaacatcgc cttgatgcta
      241 aacgaagttc gaattattgc gggaaaatgg cgcggtcgta aaattcaatt tccgaccgtt
      301 gcgggattac gcccaacgcc taatcgtatt cgcgaaacgc tttttaattg gctagcgccc
      361 tatattcatg gtgcacactg cctcgattta tttacgggca gtggtgcgtt gggatttgaa
      421 tctttatcgc gcggcgcagc acatgtgtta tgtctagaga aagatcgaga cgcttatgaa
      481 atgcttcgtc aaaatgccaa gcgcttaaat gcagatctca tggaaataca agaacaagat
      541 gcactcgttt ggataacaac gcagtgccag caggctttaa aactgactac accttttgat
      601 attatctttg ttgatccacc ctatgcctta aaagccttgt cgaagtgctt tgcattactt
      661 gaaaatcaag catggcttac aatcaacacc ctgatttatt tcgaatcgga tgtccctata
      721 gaattactaa ctttgcccga tacctgggca atcttaaaaa agaaaaaagc aggtagtgtt
      781 tattactatt taacacagaa aatcaaagat caagcttaaa aaccttgccg ctttctctta
      841 cgccctacaa atacaccaaa tatcaaccgt tgtaacaccg gcttctttta aaacttgcgt
      901 taaagcattc acagtgcttc ctgtggtcac cacatcatcc ataatcgcaa catgctcaaa
      961 gggtattggt tttacaattt gaaacgcgct ttttaaattt cgtttacggc gcgttttatc
     1021 taattgcgct tgtgctgtgg tctttcgaat gcgcacgcaa gcgtcaaata caacgggaat
     1081 ttttgttttt ttatgtatcg gccaaagtaa ttccaaagct tgattaaatc cccgttttcg
     1141 taagcgatca atatgcaatg gcacaggaat aatggcttca ggcaattcac gattttgagt
     1201 aactactcgc acccctccaa tgaaaatatt tttctaaaaa cacttgacac gtatttttga
     1261 tacaattttt gtttttttgt aatccctacc taaagatagt cctaaatcga ttatggtgat
     1321 cctacaagat cttaaacagc cgcttgggtc tttaaaaatg gatttggatg atttgttgca
     1381 atggtgtgca tttacgaaag ccgaaaaaat gcatgttatc attgtcgcat gggtaccatt
     1441 aatcaacaat cagtacgaga agaagccgag cggataaaaa ccgagtttga tcggctctct
     1501 gtagataaaa aaataaacag tgaaaccaag atgcttatcc gaagcatgtt tatgcttatt
     1561 aatttgctga tagctatttt ccttgaaaag actacaaaga aaaataataa aaactccagc
     1621 aagccatctt cacaaaccga aaaagatgaa tcctctctta caaatcatgg aactaacagt
     1681 aaaggtaaac cagaatctaa taccacggcc gacaacacga gaaccgtaga aagtatcacg
     1741 atagccgaag tgacgcaatg tgatgtttgt gggcaagact tgagtaacat cgcccaccaa
     1801 catgttgaaa gacgaactaa aatcgatatc atctttgaaa aagtaattga gcatattgat
     1861 gcacatgtta agctttgtcc tgactgtggt tcaactgtta aaggggtttt ccccgcaaac
     1921 atgcatggtc ccttacaata tggcaatgga ttaaaagcat atgtcataaa tctattaatt
     1981 tgccaaatga tatctctaaa ccgtgttcag aaactaatca aatcgatgat tggggaaatt
     2041 ctttctgaag caacactttt gaaattcatc ttgcgtcttc atctagcatt agcccaatgg
     2101 gaagctgatt caatagaact gctgctgata agtccggcta tgaatgttga tgaaacctct
     2161 ttgagagtcg acaaaaagaa ccactggatc catgtttatt cggctggcga tgtaacactt
     2221 aagcttttgc acccaaggcg cggaacagaa gcaataaatg acattaatat tattccgcga
     2281 tatagcggaa cgattatcca tgattgctgg tcatcttatt taacatacaa tgactgtgat
     2341 catggtctgt gtggctctca cttggtacgt gaacttacgt gcataatcga atctaacgga
     2401 tataattggg cagtcaatat gaaagagctt cttcttgaga cgtgcaaaat agtatcaaaa
     2461 agaaaaagga aaaagctcag cgaaggggaa tatgctaatt tgcagaagcg ctacagaaac
     2521 atcattacgc gtggagaaaa agaattaccc gccattcccc ctaagcccaa cggaaagcgt
     2581 ggcaaaatgg caaaatcaga tgcccataat ctgtgggaac gattaagaga ttatgaatat
     2641 tctgtcctac tattcgctaa aaaatcagag gtgtcattta cgaataatcg tgctgaaagg
     2701 gatcttcgaa tgtctaaagt gaaacagaaa gtatcaggtt gctttagaaa agagttatac
     2761 gctcaggcgt attgccgaat ctcaagctac cttcaaacaa tggctactaa aggatacaat
     2821 ccgttgatcg caattcaaat ggcacttgtg ggagagtcta tctaaaggga gtgagcagtt
     2881 acgattttga taccattgcg cttccacctg gtccgccaac agttcgccca ttacacgacc
     2941 ataagccaat tttttaccaa actttaatcc catcaccaat ttcgtaatgg gtggctgata
     3001 actaaacaac gaacacaagc gatcgaattt cggtggcaac tctctacaac tttcgcaatc
     3061 tatagaatct attccaggcg ttaaatagag cccacactta aagcagcgat cgctagacca
     3121 aggcaagacc attttgcaaa ctgaacataa atcttgcccg ctggtcgtat aggcttcgca
     3181 aaaacaacaa ataaatggta ataaccattg ttctactttt tttaacatgc gacaattatc
     3241 ctatgcattt atcacaaaac gctcacaaac ctaaattagc acttgcacgg catttcagcc
     3301 gcgctgcaca cacgtataat caggccgcta tcatacacca agaagtgggg atgcgtttac
     3361 tcgatcgtct cgatttaatt aacttagatc cggatgtgat attggatttg ggtagtggga
     3421 ctggtttttt tgcctcacac ttaacccaaa aatatcctaa agcacatatt ataaaactag
     3481 acattgccga aaacatgttg cgtttttcca ataaaaaaca ctttgctaaa cacacttctg
     3541 tttgcggaga tggtgactat ttgccactga aaaatcacag tatcgatttt gttttttcta
     3601 attgtgtttt acattggttt gtcaatccgc aaaaagtctt agcagaaatt cgccgcgtct
     3661 taaaaccaga aggattattg ttgttttcga ccttcggccc tgatacttta caagagctac
     3721 gcgaaagttt ttcgatcatt gatcaaaata cacatgtgaa tgcctttatg gatatgcatc
     3781 acattggcga catgctcatt caaacccaag tgcttgaccc ggtcatggat atggaaatga
     3841 taacactcac ttaccaaaac ttaatgcatc tgatacagga tctgaaagat accggagaaa
     3901 actatgttaa aagagaaaaa tccatcggac tgagccctaa aggcactgtc gataaattaa
     3961 ttaacgcgta tgagcattat cgcacgagag agggctttct accggcaact ttcgaggtta
     4021 tttatggaca tgcttggtgt tctggaagca ctacactgca tcaagcggac gaagaaggta
     4081 ttattcattt tccaattgca gatttacaaa ttctctaatt aatgcgcatt cactcaaata
     4141 ttctatctat atcatattct ttcaacaatg catctatttt aggagctctg ccacaaaagg
     4201 caacgaataa atccatacat tcctctgcgc cgccttttga taaaatcgtt tctacaaatt
     4261 ttttcgaaac atctttatta aatacttgat tatcggctgt aaactgctca aatacatctg
     4321 ctgctaaaac ttctgaccac aaataactgt agtatccggc cgcataaccg ccaccaaaaa
     4381 tatgcgaaaa cgtattttga tagtgattaa atttaggcac aggcataacc gctgtttttg
     4441 atctaacgtg atctaaaatc ttttgtatat ccatgttctt actaagatct gcgctaaatg
     4501 ttttgtgcaa aacaaaatca aacagtgaaa attctaattg gcgcattagt cttaaaccaa
     4561 tctgaaagtt tttggtggct tttaacttta aaaatagatc gtgtggtaag ggatctttcg
     4621 tttgaatgtg ttctgaaaat aatgccaagg cttcttcttc ccaacaccaa tattctagta
     4681 attgactggg tagttctata gcatcccaag aaaccccgtt cccaccagaa atactcggat
     4741 aatcgaccgt tgttaaaaga taatgcaaac agtgtccaaa ttcatgaaac aatgttatca
     4801 cttcttcatg ggttaataaa acagatccac ctgtttgggt cgatgaaaaa ttcgcaacaa
     4861 gatagaccgc aggcaattgt cgttgaccat ccggatattg catgcgcgtt ttacaaccca
     4921 aaatccaagc gccacctctt ttattctggc gagcatatag atcggtataa aaat
//
LOCUS       DAORVT010000044         4961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_12012_length_4961_cov_3.618426, whole genome shotgun sequence.
ACCESSION   DAORVT010000044 DAORVT010000000
VERSION     DAORVT010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12012_length_4961_cov_3.618426"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            625..2295
                     /locus_tag="PK389_01475"
     CDS             625..2295
                     /locus_tag="PK389_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY22416.1"
                     /translation="MGRARSLFSYFVKLIIDKITLFSHDKALIFSALSTTLIAWICLW
                     LLIEFGFRLYDFLSARVYPRFQADVRKAMFEYTLSHSHQFFSDQFAGSIGSKISRMSD
                     AILSVLTIFFTIFFPVVLAFCINIVILYHAKPLFSYIMAGWFALHILITYFFTKKCAL
                     KSAIHSAAVTQLNGKIVDAFSNIANIRLFAKKRHEMHYLSTYQNQEIKTSYNLLHYNA
                     IMKVFLGALSQGFVFIMIGLGLYAWQKDLITNGELALILTSLSLIGLAWYMGMHLIKV
                     YEDIGTCKEALTLIQTPHDISDVPHAKPIDISRGEIVFDNVSFHYTRNHNLFRDKTIT
                     LHAGQKVGLVGYSGSGKTTFVNLILRLFDIESGRILIDGQDIKQITQDSLRDQIALIP
                     QDTTLFHRSLIENIRYGRLDASDEAVMAASKKAHCDEFIQHMEHGYDTLVGERGIKLS
                     GGQRQRIAIARAILKDAPILILDEATSSLDSMTEKFIQKSLKYLMHNRTTIVIAHRLS
                     TLADMDRILVFKRGQIAEDGTHKQLLAANHHYAQLWNMQADGFLPESP"
     gene            complement(2321..3094)
                     /locus_tag="PK389_01480"
     CDS             complement(2321..3094)
                     /locus_tag="PK389_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQY22417.1"
                     /translation="MNANDENESLLNIFLEEAKDLLDAISATLEKWSKDLNNKSYFAD
                     LRRDLHTLKGGARMVNQPALSLLAHETESFCDALLASRIPVDRQAYEKVCANQDQMLH
                     ILVGLFPTTFVVSTKNPIKQVNEKMKMPVTTKKPAVVLIVDDSVTIRTVTKNFLEHHN
                     FIVVTAKDGLEAFEKLAQTRPNIILLDVEMPRMDGFQFAEKMRNDLQYADIPIVMITF
                     CIGAEQKKQAEVLGIKKFMAKPYQESELLETINALLVRA"
     gene            3239..4195
                     /gene="gshB"
                     /locus_tag="PK389_01485"
     CDS             3239..4195
                     /gene="gshB"
                     /locus_tag="PK389_01485"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008290969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HQY22418.1"
                     /translation="MLERPQMGVIMDPLDTIHPEKDTTLALLAEADRRGFALYYMELD
                     ALFLQYGIAWGNCRALELHLDKNPWYSLRAPVSRPLDQFDVLLMRKDPPVNLQYLYST
                     YILEHAESNGVLIVNKPQSLRDANEKLFATWFPQCMPPTLVSSSQSILKDFVETHECA
                     VFKPLDSMGGHNIFKCQRDDPNLTMIIDFLTQKESLYVMAQRFVPEITEGDKRILLID
                     GEPIPYALARIPAKNEFRGNLAAGGRGEGRELTERDWWICSQIGPLLREKGLIFVGID
                     VIGEYLTEINVTSPTCVKELEKAFEIDIAGKIIDSIMEKLKG"
     gene            4203..4769
                     /locus_tag="PK389_01490"
     CDS             4203..4769
                     /locus_tag="PK389_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005963206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HQY22419.1"
                     /translation="MFTSLKNHLLIAMPSLMDPHFFRSVTYLCEHTEGGAIGIIINQP
                     LLDLRLGEVLEQMGLRTDYPEIAGRLVFSGGPMQKERGFILHGNGTDTTWESTLKISD
                     TISLTTSPDILKSISANQGPTHTLIALGFAHWEAQQLEKEMSQNAWLYGPANFDILFH
                     MAIENRWRAASALMGVDVDRLSSDIGHA"
     gene            4762..>4961
                     /gene="ruvX"
                     /locus_tag="PK389_01495"
     CDS             4762..>4961
                     /gene="ruvX"
                     /locus_tag="PK389_01495"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HQY22420.1"
                     /translation="MPETPKKLEKIIIGFDFGLRHIGVAIGQSVTQTATPLATLTARD
                     GVPNWSEVQEILEKWHPNDLVVG"
BASE COUNT         1437 a         1029 c          975 g         1520 t
ORIGIN      
        1 tgatcttcaa aatgtcgatg gaaattttaa aaaaattatt gaccaacaaa aagttatttt
       61 aaattttgac taaaacaggg tatgcctttc agaattcagc ttcaatcccg tttgacaacc
      121 acaaccgcat ttaagaaatc ctactgacat cctctgctga tcccttcaca aatctcgcaa
      181 tctttatagc aaaagtccta cataaaatag ggttcaaaag agtttatttt aaacagcttg
      241 ccatatacac tgattcctgt tgattggtaa acagaatctt gacaagtgca aggatgcacg
      301 gaatgggcag tgatgtccgg ccacgcccaa cgatcacagg gagtgttcgt tgggcgacca
      361 cacttctaac aaataagtct tttccaagaa tatgatgaat acgattcaac aatttttcgt
      421 tcgaataaag ttaacgagtg ctatataaaa acatagtatt gtagtcaact aaaaacactg
      481 ttaggatcag tagctctaac cccttatttc gtccttaaca ggaattgcaa tgtctacgct
      541 accgcacact ttgccaagct tcttttggcg ctttattaaa ccgcagcgtg gccgttttct
      601 attcttgata tacggaaacc tcggatgggc cgtgcaagaa gcctcttttc ctattttgtt
      661 aaactgatta tcgataaaat tactttgttc tcacatgaca aggcacttat tttttcggca
      721 ctgagcacca ccttaattgc ttggatttgc ctttggttat tgatagagtt tgggtttcgg
      781 ttgtatgact tcctttctgc tcgcgtttac ccgcgatttc aagccgatgt acgaaaagcg
      841 atgtttgaat ataccctttc gcattcacac caattttttt ctgatcaatt cgctggcagc
      901 attggcagta aaatttcgcg catgtctgat gcgatcttaa gtgtcttaac gatttttttt
      961 accatttttt tcccagtcgt tctggccttt tgtattaata ttgtcatttt ataccatgcc
     1021 aaaccactct tttcttatat tatggcggga tggtttgcat tacatatatt gatcacctat
     1081 ttcttcacaa aaaaatgtgc tctaaaatct gccattcatt ctgctgccgt cacacaactc
     1141 aatggcaaaa tagtggatgc tttttcgaat atcgcaaata ttcgattgtt tgccaaaaaa
     1201 cgccacgaga tgcattatct ttcgacctat caaaatcaag aaatcaaaac atcgtacaac
     1261 ttgttacact ataacgcgat tatgaaagtg tttttaggtg ctctcagtca aggttttgtt
     1321 ttcattatga ttggattggg gctttatgcc tggcaaaaag acttaatcac aaacggggaa
     1381 ctggctttaa tcttgacttc gctcagctta atcggcttgg cctggtacat gggcatgcat
     1441 ttaattaaag tgtatgaaga tataggcact tgtaaagaag ccctgacatt aatacaaacg
     1501 ccacatgata ttagcgatgt cccccatgca aaacctattg atatctcgcg tggagaaatt
     1561 gtgtttgaca atgtttcttt ccactatact cgaaaccata acctttttag agataaaacg
     1621 attacactgc atgcaggcca aaaagtcggt ttggtaggat actcgggctc gggaaaaacc
     1681 acttttgtca atcttatttt aagattgttt gatatagaaa gcgggcgtat tttaattgac
     1741 ggccaagata ttaaacagat aacacaagat tcactgcgtg atcagattgc cttaattcca
     1801 caggatacca cacttttcca tcgctctttg atagagaaca ttcgatacgg tcgtctcgat
     1861 gcttctgatg aagcggtaat ggctgcctct aaaaaagcac attgcgacga atttattcag
     1921 catatggaac acgggtacga tactttagtc ggagagcgcg gcattaagct ttcgggtggt
     1981 cagcgtcaac gcattgcaat tgcgcgtgcg atattaaaag atgcaccgat tttaatttta
     2041 gatgaggcaa cctcttcatt ggattcgatg acagaaaaat ttattcaaaa aagcctcaag
     2101 tatttaatgc acaaccgcac cacgattgtg atagcacatc ggctttcaac gcttgcagat
     2161 atggatcgca ttttagtatt caagcgcggt cagattgctg aagatgggac tcacaagcaa
     2221 ttgcttgccg caaatcatca ctacgcacaa ctgtggaata tgcaggcaga tggattttta
     2281 ccggaatcac cctaatgcta aacgtcaaag ttatttttgc ttaagccctt actagcagcg
     2341 cattaatggt ttctaacaac tctgattcct ggtaaggttt agccataaac ttttttatac
     2401 ccaggacttc agcctgcttt ttctgttccg cgccaataca aaatgtaatc atgacaatcg
     2461 gaatatctgc atactgaaga tcattacgca ttttttctgc aaactgaaag ccgtccattc
     2521 ttggcatttc gacatcaagc aaaataatat tgggtcgcgt ttgtgctagt ttttcgaatg
     2581 cctctaagcc atctttagca gtaacaacga tgaaattatg atgttctaga aaatttttag
     2641 tcaccgtacg aatagtcaca gagtcatcga caatcaaaac aacagccggc tttttagttg
     2701 ttactggcat tttcattttt tcattgacct gttttatggg atttttcgtt gacacaacga
     2761 atgttgttgg aaatagccct actagaatat gtagcatctg atcttgattg gcgcatactt
     2821 tttcgtatgc ttgtctatcc acaggaattc tgctcgctaa aagcgcatcg caaaaagact
     2881 ctgtctcgtg cgcgagcaaa cttaaggcgg gttgattaac catgcgcgca cctcctttca
     2941 aggtatgcag atcgcgcctt agatcagcaa aataggattt attattcaaa tcctttgacc
     3001 atttctctaa agttgcagaa atagcatcta acaaatcttt ggcttcttcc aaaaatatgt
     3061 tcagcaacga ttcattttca tcatttgcat tcatgtcatg ttatccgttt gtcgttccta
     3121 ttcttgcaag gttgtttgac ctaaaaccaa taccgaatca atccttttct gttactgaat
     3181 tttgagatat tatcagaagt atagcttagg ttcatgcctt taatctaaag gattattcat
     3241 gcttgaaaga ccccaaatgg gcgtaattat ggatcccctc gataccattc accctgaaaa
     3301 ggacacgacc ttggcgcttt tggcagaggc tgaccgtcgg ggatttgcgc tttattacat
     3361 ggagctcgat gctctttttt tacaatatgg cattgcatgg ggaaattgcc gagctttaga
     3421 actacatttg gacaaaaacc cctggtattc gctccgagcg cccgtcagta gaccgctgga
     3481 tcaattcgac gtattgttaa tgcgtaaaga tccacccgtc aacctacagt acctctattc
     3541 gacctatatc ttagagcatg ccgaatctaa cggcgtctta attgttaaca aaccacaaag
     3601 cttaagagat gcaaatgaaa aactttttgc aacgtggttt cctcaatgca tgccgccgac
     3661 actggtcagc tcttctcaat ccatcttgaa agattttgtt gaaacacatg agtgcgccgt
     3721 ttttaagcct ttagacagta tgggtggaca caatattttc aagtgtcaac gggatgatcc
     3781 aaacctaacc atgattatcg attttctcac acagaaagaa tcactttatg tgatggctca
     3841 acgatttgtg ccagaaataa cggagggcga taagcgaata ctcttaatcg acggagaacc
     3901 cattccttat gcgcttgcca gaattccagc taaaaacgaa tttcgcggaa atttagctgc
     3961 gggtggccgt ggggaaggtc gagaattaac tgagcgtgat tggtggattt gctctcaaat
     4021 tggcccattg ttgcgcgaaa aagggctgat ctttgtcggc attgatgtga ttggcgaata
     4081 tttgactgag atcaatgtaa caagcccaac ctgtgttaaa gaattggaaa aggcatttga
     4141 gatcgatatt gcaggaaaaa ttatagattc tatcatggaa aaattaaaag gataatcaat
     4201 atatgttcac ttcacttaaa aatcatttac taattgccat gccttcttta atggatccgc
     4261 atttttttcg atctgtaact tatctttgtg aacatactga gggtggcgct atcggcatta
     4321 tcattaacca accactgtta gacttaagat taggcgaagt tttagaacaa atgggcttaa
     4381 gaacagatta ccctgaaatt gcaggtcgtt tggtatttag cggtgggccc atgcaaaaag
     4441 aacgagggtt tattttgcat ggaaatggaa ctgacacgac atgggaatcc acgctgaaaa
     4501 ttagcgacac aatatcgctc acaacctctc ctgacatttt aaaatcgatt tcagctaatc
     4561 aaggtccaac gcatacatta attgcactcg ggtttgcaca ttgggaagct cagcaattgg
     4621 aaaaagaaat gtctcaaaat gcatggttat atggtccggc taattttgat attttatttc
     4681 atatggccat tgaaaatcgg tggcgagctg cctccgcctt aatgggcgtt gacgtagatc
     4741 gcctctcgag tgatattgga catgcctgaa acccctaaaa aactcgaaaa aattattatt
     4801 ggctttgatt ttggcttacg acatatcggc gttgccattg gacaatcggt tacccaaacg
     4861 gcgaccccgc ttgcaacact gaccgcgcgt gatggtgttc ctaactggag cgaggttcaa
     4921 gaaattttgg aaaaatggca tcccaatgat cttgttgtag g
//
LOCUS       DAORVT010000045         4918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_12181_length_4918_cov_3.763109, whole genome shotgun sequence.
ACCESSION   DAORVT010000045 DAORVT010000000
VERSION     DAORVT010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12181_length_4918_cov_3.763109"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1342)
                     /locus_tag="PK389_01500"
     CDS             complement(<1..1342)
                     /locus_tag="PK389_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22421.1"
                     /translation="MHNGQKNKDNAEQKNDQPQVNALVTTTAATTSTCLTRSARHSIS
                     PRALTAEITTCTQATAELIALSRDLYSLLATEGPNIARIPEILTRRLSTLFPFAAGTD
                     MLSKIQSATTQLMTFGVNPALALHGVKKAMGAGVGKIDQGKHVLASAIHSAGGPASDA
                     LDLYHTCAGLYSFYNTYTELNRLYASILFCSQRVYTSAQELAELTGTNEQWGMINEVF
                     LEYWDQPGFDFNAFINQTAEQFKIMLDPLKGLIVLYETMEPVRMATYLAITMRAEPNA
                     ESQLDQVTYLLTNACYNTALDNLSVVSKGAGDLLGRELLGIAVNAGTTAALTTCAITV
                     TGMNFPITIAILATCITNPSKRDALITYAKSLIMNPQVGETKSDTGVRATVLKQLEAL
                     KNRGNASLSPLAENISPKINQLMKKCLDASGAVVGNITENAIRTLMRDSSLTPFL"
     gene            complement(1990..2691)
                     /locus_tag="PK389_01505"
     CDS             complement(1990..2691)
                     /locus_tag="PK389_01505"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQY22422.1"
                     /translation="MIFRNFLFYLGLVPATLLFAILGTLFFFLPFKARYYLITRWSHF
                     FIFWAKITCGLRYKVTGKENIPTKNAIIFANHQSMWETIFMQVLFPTQSWVLKKELLK
                     IPVFGWGLALLDPIAIDRKQFNSVKALIQQGFERLQKGRWVVIFPEGTRVAPNMNRPY
                     SRSGAALAEATGYPILPVAHNAGLYWPRGFFIKKPGTIQVVIGPIIESKEKTATVLNK
                     EAEHWIRETLETIVK"
     gene            complement(2688..3245)
                     /gene="gmhB"
                     /locus_tag="PK389_01510"
     CDS             complement(2688..3245)
                     /gene="gmhB"
                     /locus_tag="PK389_01510"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HQY22423.1"
                     /translation="MKPLVILDRDGVINVESPDFIKTPEEWHPIPGSLEAIVKLNHAG
                     YWVAVATNQSGVGRGLFDLAMLESIHQKMRDALAKLGGHVDLVLFCPHLPDSHCECRK
                     PKPKLMNDIATHFKIDLKKTPVPAIGDSLRDLIAAQTAGCKPLLVLTGNGQRTHENLP
                     ADLKQIAVYSDLASAIDALLMSKAP"
     gene            complement(3242..>4918)
                     /gene="glyS"
                     /locus_tag="PK389_01515"
     CDS             complement(3242..>4918)
                     /gene="glyS"
                     /locus_tag="PK389_01515"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HQY22424.1"
                     /translation="LTEALNALPFGRRMRWGTQTDSFVRPIHWIVLLWDHKVIQTEIF
                     GVKSSNLTYGHRFHHPEALPISDPKDYESVLNKGFVIANFEKRREKILSEITRITTEK
                     KINAIIEDTLLDEVTGLVEWPVVLLGSFDKAFLDVPREALITSMQTHQRCFPVEDQSQ
                     KLLPHFLLVSNINSKNPAAVVNGNECVIAARLADAVFYYRLDKEIPLHQRLIDLKQVR
                     FQWGLGTLWDKSERLAKLAMLIAPKIHADPLYTERAGLLCKTDLLTQMVGEFPELQSI
                     MGCYYALHDSEPVAVAMAIEDHAHPRFAQDTLPTTPEGAALALADRLDSLVGIFGLGN
                     RPTGDKDPFALRRQALGILRILIEKQCDCDLNDLLHVAKEQYAHQAITLSEPEVISQV
                     LDFCFERLRALYQDKGINPRVFEAVFAKRITNILDFDKRIQAINYFITLKEAESLAAA
                     NKRVQNILSKCDTDIFRDLTVQNDLLTENAEKTLWLQLAAKEKEVAPLLQVGNYTEAL
                     KSLATLREPIDQFFTDVMVMVDDLTLRNNRLRLLHRLRVLFLEIADISLL"
BASE COUNT         1411 a          974 c         1015 g         1518 t
ORIGIN      
        1 ttaaaaaagg cgtcaaacta ctatctcgca ttaaagtgcg tatcgcattt tcagtaatat
       61 ttccgacaac agcaccgctc gcgtcaagac actttttcat cagttgatta attttgggcg
      121 aaatattctc agccaagggg cttagcgaag cattgccgcg atttttgagt gcttccaact
      181 gcttcaatac ggtagcgcga acgcctgtat ccgattttgt ctcaccaact tgtggattca
      241 tgatgaggct ttttgcataa gtgatcaaag cgtctctttt gcttggattt gtaatacaag
      301 tagccaatat agcaattgta atcggaaaat tcatgccagt gactgttatt gcacaagtcg
      361 ttaatgcagc tgttgtgccg gcgtttactg ctataccgag tagctctctg cccaagagat
      421 cacccgcacc tttagaaaca acactgaggt tatctagagc agtattataa caagcattcg
      481 ttagcaaata ggtgacttga tctaactgtg attctgcatt aggttctgca cgcatcgtga
      541 tagctaaata cgttgccatt cgaacaggtt ccattgtttc gtataaaaca atcagtcctt
      601 ttaaggggtc aagcattatt ttaaactgtt ctgcagtttg gttaataaaa gcattaaaat
      661 caaatccggg ttgatcccaa tactcaagaa acacttcgtt tatcatgccc cattgctcgt
      721 tagtacccgt taattctgcg agctcttgtg cgcttgtgta gactctttga gaacaaaata
      781 gaatagaagc atataaccta ttgagttctg tgtacgtgtt ataaaaacta tataagcctg
      841 cacaggtatg ataaagatcc aatgcatcag aagcgggtcc tcccgcacta tggattgcgc
      901 ttgctaagac atgttttcct tgatctattt ttccaacacc cgctcccatt gcttttttaa
      961 cgccatggag tgctaaggct ggattaactc caaaggtcat taattgtgtg gttgcagatt
     1021 gtattttaga aagcatgtct gtgccagcag caaatggaaa taatgtagat agccgccttg
     1081 ttagtatctc ggggatgcgt gcaatattag ggccttcggt tgccagtaag ctatacagat
     1141 cccgactgag tgcgattaat tcagctgtgg cttgcgtaca ggtggtaatt tctgctgtca
     1201 gtgctcgagg actaatgcta tggcgagccg agcgtgttaa gcatgtggag gttgttgctg
     1261 ctgtggttgt gactagagca ttcacttgag gctgatcatt tttttgttca gcgttatctt
     1321 tatttttttg accattgtgc atatcaatcc ccttgtatct tattaaatgg cagctaaatt
     1381 cagagcggtt ttcgcattat tttcgtgcag ttacacgatg tttaatttat gtctgtgaga
     1441 ttttagaaaa tctaaaggcg agcgatacta tcaacatatt caatctccga gacattaaca
     1501 tttttattac ttctaagtat gacgctaaca cacttaaaat tcaagataga taggattttt
     1561 attttcaccc taatttttaa agtatttttt agtgcatata caactagaga aaacacggtt
     1621 aaagtgtttt atttttctca taaatgccgc agaagaacat ttttattact tttaaatatg
     1681 gcgataaaat atttagattt taatcctgtg caataagggt gttcatatat atcattagca
     1741 cacaatatgt aataaaaaaa acgatagatt gtattatttt atttataaac aacctatcgc
     1801 ttaacaattg catcattgta cttatgaata acctatcatc gaacgtgtgc atgtcaatct
     1861 cctgtattgc atgaacttat tttctatttt acagaaagca aattaagttt ctacccgtgc
     1921 gatcaagggg atgttgaaaa taaatttcta attgacaatt tagggtaggt caaattatta
     1981 gctgggtgtt cattttacga tagtttccag cgtttcccta atccaatgtt ctgcttcttt
     2041 atttaatacg gttgctgttt tttctttaga ctcaataata ggcccaatca caacctgaat
     2101 cgttccgggt tttttaataa aaaatccgcg cggccaatac agtcctgcat tgtgtgcaac
     2161 aggcaatata ggatatcccg tggcctctgc aagcgctgcg ccgctgcgag aataaggacg
     2221 gttcatattg ggagcaacgc gggtgccttc tgggaaaata acaacccagc gacctttttg
     2281 taaacgctcg aaaccttgct gaattaaagc cttaacagaa ttaaattgtt ttctgtcaat
     2341 cgcgatcggg tcgagtaatg caagccccca tccaaaaaca ggaattttta ataattcttt
     2401 tttaagtacc caggattgtg ttggaaacaa tacctgcata aaaatggttt cccacatgga
     2461 ttgatgattt gcaaaaatga ttgcattttt tgtgggtata ttttctttcc ccgtaacctt
     2521 atagcgaaga ccacaggtta ttttggccca aaagataaaa aaatgcgacc aacgggtaat
     2581 taaatagtac cgtgctttaa agggcagaaa aaaaaacagc gttccaagta tggcaaaaag
     2641 aagtgtggct ggaacgagac ccaggtaaaa cagaaaattt cgaaatatca cggtgctttg
     2701 ctcatcaaca gtgcatctat ggcggatgct aaatcagaat aaacggctat ttgtttgaga
     2761 tcggcgggaa ggttttcatg tgtgcgttgt ccattgcctg ttaaaaccaa aagtggtttg
     2821 catccagctg tttgagcagc aattaaatct cgcagagagt ctccaatggc gggaacaggt
     2881 gttttcttga gatcaatttt aaaatgtgtt gcaatatcat tcatgagctt aggctttggt
     2941 tttcggcatt cacagtgtga gtcgggcaga tgtgggcaaa atagcacaag atcaacgtgc
     3001 ccccccaact ttgctaaagc gtctcgcatt ttttgatgta tggactctaa catcgcgaga
     3061 tcaaacaacc cacggcccac tcccgactgg ttcgttgcaa ctgcaaccca ataacccgca
     3121 tgattgagtt ttacaatggc ctctaggctg ccgggaatgg ggtgccattc ttcaggggtt
     3181 ttaataaaat ctggcgattc aacattaata accccatctc tgtctaaaat gacaagcggt
     3241 ttcataataa ggaaatatcc gcgatttcta aaaataagac tcgcaaacga tgcagtaatc
     3301 ttaagcgatt attacgtagc gtgagatcat caaccatcac cataacgtct gtaaaaaatt
     3361 gatcaatggg ctctcttaat gttgccaagg attttagggc ttctgtgtaa ttcccgacct
     3421 gcaagagtgg cgcaacttct ttttctttag cggccaactg aagccataat gttttttcag
     3481 cattttctgt caacaaatca ttttggaccg ttagatctct aaagatatct gtatcacatt
     3541 tggataaaat attttgtaca cgcttattgg ctgctgccaa actttcggct tcttttaagg
     3601 taatgaaata attgatagct tgtatgcgtt tatcaaaatc taggatattg gtaattcgtt
     3661 ttgcaaagac agcttcgaaa acacgcgggt taatgccttt atcttggtat aaggctctta
     3721 ggcgttcaaa acagaaatcc agcacctgcg agataacttc tggttcggat agcgtgattg
     3781 cttggtgtgc atactgttct ttagcaacat gcagcaaatc gttgagatca cagtcacact
     3841 gtttttcgat caaaatacgt aaaataccca gtgcttgacg tcggagtgca aatggatctt
     3901 tgtctcctgt tggacgattg cccagcccaa agattccgac taaagaatcc agccgatccg
     3961 cgagcgcaag tgcagctccc tctggtgttg tgggcaaggt atcttgcgca aaacgaggat
     4021 gtgcatgatc ttcaatcgcc attgcaacag caacaggttc ggaatcatgt aaggcatagt
     4081 agcaacccat aatgctttgt aattctggaa attcaccgac catttgtgtt aataaatcgg
     4141 ttttgcagag taaccctgcg cgttcggtat acaagggatc cgcgtgaatt ttaggcgcaa
     4201 tcaacatggc aagttttgca aggcgttctg atttatccca taaagtgcca agcccccatt
     4261 ggaagcggac ttgttttaaa tcgataagac gctgatgcaa ggggatttct ttgtcaaggc
     4321 gataataaaa aacagcatca gcaagacgcg ctgctataac gcattcgttt ccattaacaa
     4381 ctgcggctgg atttttactg ttgatattac ttaccaataa aaagtgtggt agtaatttct
     4441 gagattgatc ttcaacgggg aaacagcgtt gatgtgtttg catagaggta attagcgctt
     4501 ctctgggaac atctaaaaaa gccttatcaa aagagccgag taatacaaca ggccattcaa
     4561 caagacctgt cacctcgtcg agcaaggtat cctcaataat ggcattgatt tttttttctg
     4621 tcgtgattct cgtgatttca gaaagaattt tttcgcgtcg tttctcaaaa ttggcgatga
     4681 caaagccttt gtttaaaaca gattcatagt cttttggatc agaaataggc agcgcttctg
     4741 gatgatgaaa gcggtgccca taagttaaat tgctgctttt aacgccgaat atttcggttt
     4801 gtataacttt gtgatcccat aaaagcacaa tccaatgaat gggtcgtaca aaactgtctg
     4861 tctgtgttcc ccaacgcata cgtctgccaa aaggtagcgc atttaaggct tctgtgag
//
LOCUS       DAORVT010000046         4918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_12182_length_4918_cov_3.224347, whole genome shotgun sequence.
ACCESSION   DAORVT010000046 DAORVT010000000
VERSION     DAORVT010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12182_length_4918_cov_3.224347"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /locus_tag="PK389_01520"
     CDS             complement(<1..1182)
                     /locus_tag="PK389_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQY22425.1"
                     /translation="MSNITLSLYMAIGLLIAVSFVYLIWLYIHFKKLLAVLSTTIEKV
                     VHFSNADPLTGLPNRTQFFENLKNKIALPREKHQKLALFFLDVDNFRGIKDSLGYEIG
                     DGLLQAIAKRLSISFSNKRELFARLGSDQFTIMIDELDTQNSIDDEARRILALLAQPF
                     LIQGHEIRTTASIGISVYPEDSENIESLLKFAGIARHQAKKIGSNTFSYYTLAMNTEI
                     ATKRTIEVHLRHAIANKELFVYYQPKVDVRTRKIVGAEALLQWINPELGRISPAQFIP
                     LAEQTGLIINIGNWALKEACVQTKKWHDQGFNDFVIAVNLSAYQFKMGDIAEQVASAL
                     WDSQLNANFLELELTESLVMDNMEKSLLMLRVLKSMGIKISIDDFGTGYSSLNHIREM
                     PL"
     gene            1310..4069
                     /gene="polA"
                     /locus_tag="PK389_01525"
     CDS             1310..4069
                     /gene="polA"
                     /locus_tag="PK389_01525"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HQY22426.1"
                     /translation="MPVIPKNPFILVDGSSYLFRAYHALPPLTTSKGLPTGAIYGVVN
                     MLHKLIKTYHPEKMAVVFDSKEKNFRHALYEPYKANRTVMPDELQQQIEPLHAIIQAM
                     GLPLVVIPGIEADDIIGTLAEQAKTNGIFTLISTGDKDFAQLVCEEIFLVNTMSEDIF
                     DRARVIEKFEVPPEQIIDYLTLIGDTVDNIPGVPKVGPKTAVKWLQTYGSLQAVVAHA
                     LDIPGKVGENLRDALEQLPLFKELVTIQVHLPVEHKVADFVLQKREIEKLKNLFSELE
                     FKNWLKDLNRSEAENKSETTKELQATKEDLKNNNTEKRVENLAIYETILEKTVFEKWL
                     KKLENSELFAFDTETTSLDYMLAEIVGLSFAVTSGEAAYLPVGHNYMGAPLQLNREWV
                     LEQLRPVLEDKKRFKVGHNLKYDSEVLANHGIMLQGIAFDTMLESYVLDSTATRHDMD
                     TLAERHLHYKTISFSEVAGKGAKQITFNQVPLESAAFYAAEDADVTLKLHQKFWPLLQ
                     QLPKQKNVFETIEMPLVPVLVRMERGGVLIDSQCLAAQSVVLGKRLAEIETEAYQMAG
                     QSFNINSPKQLQEILYTQLKCPVLEKTPTGQPSTAESVMQQLAEDYPLPKIILEYRSL
                     SKLKSTYADKLPQQINAKTGRVHTSYHQAITSTGRLSSSDPNLQNIPIRSPLGRKIRS
                     AFVAPAGYKIVAADYSQIELRIMAHMSQDQGLLKAFSLGDDVHKSTAAAVFGVSLEAV
                     TSDQRRSAKAINFGLMYGMSAFGLAKQLGISRGDAEKQVERYFACFPGVRVFMEEIRK
                     RALEEGFVETLTGRRLYLPDLKSSYMATRRAAERAAINAPMQGTNADIIKLAMIQLDQ
                     WILQNSLDMRMIMQVHDELVFEVSEKILDKALSHIKTIMETILPLSVPLKVGMGVGDN
                     WDEAH"
     gene            complement(4151..4762)
                     /gene="yihA"
                     /locus_tag="PK389_01530"
     CDS             complement(4151..4762)
                     /gene="yihA"
                     /locus_tag="PK389_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016855872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HQY22427.1"
                     /translation="MSILNYNQIAFLKSANSKAGLPNDSGAEVAFGGRSNAGKSSALN
                     KITGIHRLARTSKTPGRTQLINFFQLADPEKRLVDLPGYGYAKVPDSIKKHWQTIVSQ
                     YLTERKSLKGLILLMDIRHPLKAQDWQMIEWSLAHQLPTHILLTKADKLGRGAARNTL
                     LMLKKELLGYEEFISSQLFSMLSGEGIVEVQEKLDEWLQAEHA"
BASE COUNT         1458 a          910 c         1046 g         1504 t
ORIGIN      
        1 aagaggcatc tccctgatat ggttgagtga tgaatatcct gttccaaaat catcaatgga
       61 aattttaatg cccatgcttt ttagaacacg tagcatcagt aaagattttt ccatattgtc
      121 catgacgagg ctttcagtta actcgagttc tagaaaatta gcgtttaatt ggctgtccca
      181 aagtgcgctt gcaacttgtt ctgcaatatc tcccatttta aactggtaag ccgaaaggtt
      241 aacggcaatc acgaaatcgt taaacccttg gtcgtgccat tttttagttt gcacacacgc
      301 ttcttttaaa gcccaattac caatgttaat aataagccct gtttgttcgg caagtggaat
      361 aaattgtgcc ggagatattc tccccagctc agggttaatc cactgcagca gcgcctctgc
      421 gcccactatt ttgcgtgttc taacatctac tttgggttga taataaacaa agagttcttt
      481 attggcaatg gcatgtcgta aatgcacttc tattgttcgt tttgtagcta tttcggtatt
      541 cattgcgagt gtataataac taaatgtatt gctgccgatt tttttggctt gatgacgtgc
      601 gatgccagca aattttaaca aagattcaat gttttcgctg tcttcgggat aaacactaat
      661 tccaatagaa gcagttgttc ttatttcatg cccttgtatt aaaaaaggtt gcgctagtaa
      721 agccaatatt cttctggcct catcgtctat gctattttgt gtgtcaagct catcaatcat
      781 aattgtaaat tgatcgctgc ctaggcgcgc gaataattct cgtttatttg aaaagctaat
      841 gcttaaacgt ttggcaatgg cttgtagtaa tccatctcca atttcgtaac ctaaagaatc
      901 tttgatgcct cgaaaattat caacatctaa aaagaaaagc gcaagctttt gatgtttttc
      961 tctcggtagt gcaatcttat tttttaagtt ttcgaaaaat tgcgttcgat tgggcaagcc
     1021 cgttaaagga tccgcattag aaaaatgcac gactttttca atggtagtgc tgagcactgc
     1081 taataatttc ttaaagtgta tgtagagcca aattaaatag acaaatgaaa cagcaatcag
     1141 aagacctatt gccatatata aacttaaggt gatattactc attgtctatt cctgagttct
     1201 tatgaccaag atggattaat tcgcaagagt ctgttcaatg actactttat aagtatagta
     1261 gagataggca taatgtgtta ttgatattcc taaaatagag tcttaaacaa tgcctgttat
     1321 tccaaaaaat ccgtttatct tggtggatgg ttcttcctat ttatttcgcg catatcatgc
     1381 gctcccgcct cttaccactt ctaaaggtct gccaacaggc gcgatttatg gggtggttaa
     1441 tatgttacac aaacttatca aaacctatca ccctgaaaaa atggccgttg tgtttgacag
     1501 taaagagaaa aattttcgac atgcgctcta cgagccctat aaagccaatc ggactgtgat
     1561 gcccgatgaa ttgcagcaac aaatagaacc attgcatgcc attattcaag cgatgggttt
     1621 gccattggtg gttattcccg ggattgaagc ggatgatatt attggcacgc tggcagagca
     1681 ggcaaaaacg aacggcatct ttactttaat ttctacaggt gataaagatt ttgcacaact
     1741 ggtgtgcgaa gaaatttttt tagttaatac catgtcagag gatatttttg atcgtgctcg
     1801 cgtgatagaa aaatttgaag taccacccga acaaatcata gattatttaa cattaatagg
     1861 cgacaccgtg gataacatac ccggtgtgcc aaaagtgggg ccaaaaaccg ccgttaaatg
     1921 gctgcaaact tatggcagtt tgcaagcagt tgttgctcat gccttggata tccctggaaa
     1981 agttggcgaa aatctccgcg atgctttaga gcagcttccc ttgtttaagg agcttgttac
     2041 gattcaagtc catttacccg ttgagcataa agtggctgat tttgttctcc aaaaacgaga
     2101 gatagaaaaa ttaaaaaatt tattttcaga gctggaattt aaaaactggt taaaagactt
     2161 aaatagatct gaagcagaaa ataaatcaga gacgacaaaa gagctgcaag caacaaaaga
     2221 agatttgaaa aataataata ctgaaaagcg cgtagagaat ttagcaattt atgaaacaat
     2281 tttagaaaaa accgtttttg aaaaatggct gaaaaaactt gaaaattcag aattgtttgc
     2341 ttttgataca gaaacaacca gtttggatta tatgttggca gaaattgtag gattgtcttt
     2401 tgccgttaca tcgggcgaag ctgcttatct tccggtagga cataattaca tgggtgcgcc
     2461 actgcaatta aatagagaat gggtattgga acaattacgc cccgtgttag aagataaaaa
     2521 acgattcaaa gtagggcata atttgaagta tgatagtgaa gtgttggcga atcatggcat
     2581 tatgttacag ggtatcgctt ttgacaccat gctggaatct tatgtgctag atagcacggc
     2641 aacgcggcat gatatggata ctttagcaga acgtcatttg cactataaaa caatctcttt
     2701 ttcagaagtg gctggcaaag gtgccaaaca aatcacattt aaccaagtgc ctctagaaag
     2761 cgctgctttt tatgcggctg aagatgcaga tgttacacta aaattacatc aaaaattttg
     2821 gccattactg caacaattgc caaaacaaaa aaacgttttt gaaacaattg agatgccatt
     2881 ggtacccgtt ttggttcgta tggagcgcgg tggcgtttta attgattcgc aatgcttggc
     2941 tgctcaaagt gtcgtcttag gcaaacgctt ggcagaaata gaaacagaag cttatcagat
     3001 ggcgggtcag tcttttaata ttaattcgcc taaacaacta caagaaatat tatacacaca
     3061 attaaaatgt cctgttttag aaaaaacgcc taccggccag ccttctaccg ccgaatctgt
     3121 tatgcagcaa ttggcggaag attatcctct gccgaaaatt attttagaat atcgcagttt
     3181 aagtaaatta aaatccacgt atgcagataa gctacctcaa cagataaatg caaaaacagg
     3241 acgtgtgcat acttcgtatc atcaagcgat tacttcgaca ggacgacttt cttccagtga
     3301 tcctaattta caaaacattc cgattaggtc cccgctgggc agaaaaattc gcagcgcttt
     3361 tgttgcgcca gcgggttaca aaatagttgc tgcggattat tctcaaattg aattgcggat
     3421 tatggcgcat atgtctcaag atcaaggatt attaaaagcg tttagtttgg gagatgatgt
     3481 tcataaaagt actgctgccg ctgtctttgg tgtaagttta gaggcggtaa cgtctgatca
     3541 acgacgcagt gccaaagcga ttaattttgg tttgatgtat gggatgtcgg cttttggttt
     3601 ggcaaaacag ctgggtattt cgcgagggga tgcagaaaaa caggtagagc gatactttgc
     3661 ttgttttcca ggtgttaggg tatttatgga agagattcgg aaacgtgcac tggaagaggg
     3721 ttttgtagaa acgttaacgg gtcggcgttt atatcttccc gacttaaaat catcctatat
     3781 ggcgacacgt cgtgcagcag aacgtgccgc aattaatgcg ccgatgcaag gcaccaatgc
     3841 cgatattatt aaattagcca tgattcaact agatcagtgg attttacaaa atagcttgga
     3901 tatgcggatg attatgcagg tgcacgatga attagtgttt gaggtatcag aaaaaatatt
     3961 ggacaaagca ctgtctcata ttaaaacgat tatggaaaca atcctgccgc tttcagtgcc
     4021 tttaaaagta ggaatgggtg tgggagacaa ttgggatgag gcgcattagg ctctgcgcaa
     4081 aaataattgt aactatgtct gtttttacac tctcgaattg gagtctgtcc attatttcag
     4141 gagacttctt ttatgcatgt tcagcctgca accattcatc taatttttct tgcacttcca
     4201 cgatgccttc tccgcttagc attgaaaata actgagaaga aataaattct tcataaccca
     4261 gcagttcttt tttaagcatt aacaaggtat ttcgcgcagc gcctctaccg agtttatctg
     4321 cttttgttaa taagatatgt gttggcaatt ggtgtgcaag agaccactca atcatttgcc
     4381 agtcttgtgc tttaagtgga tgtcgaatat ccatcagtaa aataagacct ttaagacttt
     4441 ttctttctgt tagatattga gacacgatag tttgccagtg ttttttgata gaatctggca
     4501 ctttggcata accataacca ggtagatcta ccaggcgttt ttcaggatcc gccaactgga
     4561 aaaagttaat caactgagtt cttccaggcg ttttgctggt tctggcaagg cgatgaatgc
     4621 ctgtaatctt gtttaaggca ctggatttac cggcgttaga gcgtccgcca aatgcaacct
     4681 cagccccgct gtcgtttggt agccctgctt tgctgttggc actctttaaa aatgcgatct
     4741 ggttgtagtt taatatgctc attttgggta ttatgataac aggtttatag gaactaagtg
     4801 atgcatatct ggataagatt agcgattttt ttgggaatgg gtgtggcgtt tgccggtagc
     4861 atgcccgaga ttctccccga tattgctgct gggcgtgaga aatcccaggt gtgcgttt
//
LOCUS       DAORVT010000047         4907 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_12225_length_4907_cov_3.337593, whole genome shotgun sequence.
ACCESSION   DAORVT010000047 DAORVT010000000
VERSION     DAORVT010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4907
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12225_length_4907_cov_3.337593"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..752)
                     /locus_tag="PK389_01535"
                     /pseudo
     CDS             complement(126..752)
                     /locus_tag="PK389_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005962786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
     gene            complement(745..1710)
                     /gene="mltB"
                     /locus_tag="PK389_01540"
     CDS             complement(745..1710)
                     /gene="mltB"
                     /locus_tag="PK389_01540"
                     /inference="COORDINATES: protein motif:HMM:NF024798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HQY22428.1"
                     /translation="MLSNKFRFLQVFLFTCIFTLISAHAAALPAKTIQQNKSTSFAKR
                     KEVQQFIQSMHKKHGLNEKNLNAIFSEFGSENKVLRLMSKQYEALPWYRYRDTVITEK
                     RIQEGVQFWREHKDLLKKAEAKFGVPSEIIVAILGIETSYGKITGNYPVLQALATLAF
                     DYPRRAAFFRGELENFLLLVNEGSLDPVTTRGSFAGAIGTPQFMPSSYRRYAVDFSGT
                     GKRDLIHDMADVIGSVGNYMKKNGWKKNEKIIATLRLQEKTHPLQKGKLIVLEKNAHH
                     DKEEWIGLDNFNVILRYNRSTHYAMAVYQLSQKIRILKTRAKNND"
     gene            complement(1749..2843)
                     /gene="rodA"
                     /locus_tag="PK389_01545"
     CDS             complement(1749..2843)
                     /gene="rodA"
                     /locus_tag="PK389_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005348281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HQY22429.1"
                     /translation="MFRSNRSFISRLHLDWPLLIGIILLSALGILILYSAKPEMDVIQ
                     KQGLRLMLACGIMLFIAQIPPRFFSSIAPWLYGIALMMLISVLVMGHVSKGGQRWLDL
                     WFVRFQPAELMKLAVPLMIAKYFNDHSLPPKVSMLLIPLVLILVPTLLIAKQPDLGTA
                     LLILSSGLLVIFFSGLSWKILFSLASLVTLCLPTLWFFMRDYQRQRILVLLDPERDPL
                     GSGYQIIQSKIAIGSGGLYGKGWLNGTQSNLEFLPERTTDFIFAVFSEEFGLVGCLVL
                     LLLYLYIIIRGIYISVQAQNTFGRLLAGSLMMTLFVYVFVNIGMVTGILPVVGIPLPL
                     VSYGGTSLVTLMASFGILMSIHTHRTLLAK"
     gene            complement(2830..4770)
                     /gene="mrdA"
                     /locus_tag="PK389_01550"
     CDS             complement(2830..4770)
                     /gene="mrdA"
                     /locus_tag="PK389_01550"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HQY22430.1"
                     /translation="MRNIHTLKDHFKESQLYLNRAIIAAVLVFIVLTTLTFRLVYLQI
                     SQHDVYKTLSLNNQVRIVPITPTRGLIFDRNGVLLAENMPAFSLELTPERTPNIDDTL
                     KIINDIMPLTDTEQQQFYKQLKYKRRNESIPIRIKLSEEEVAKFSVEKHRVPGVDIVA
                     RLIRHYPFRESLAHALGYIGPISEKDLTQINTANYRGTYNIGKIGVEKFYEDVLHGTT
                     GYQHIETDAKGRTIRVLNRIPPVSGKDLYLSLDNDLQHAATEALKDLKGAIVAIDPNN
                     GEVLALVSNPAFDPNLFTQGIDVQTYAALQNSPDRPLFNRAIRGQYPPGSTVKPMVAL
                     QGLELGTVTPEFQIFDPGWYKLNGVGRMYRDWIYFARRHGHGTVDMEKAIAQSCDTYF
                     FTLAHKLGVKHLHNIFTRFGLGKITHIDTTGEASGLAPSADWKKRVYKDDWYPGDTLN
                     IGIGQGLLLATPLQMAQVAAILANRGQGFEPRFAMAIGVDETQKPIKQNTKAIPKITL
                     KKDANWDIIIKGMQKVVQPGGTANRISHGLTYTVAGKTGTSQVFNLKQHEKYEVNKIK
                     AYLRDHSWFIAFAPVEKPQIALAVLVENKYKKTGADVARIVLDSYFHSKSAMATSDKI
                     DDADDESEVGGEENSGEENVSE"
BASE COUNT         1404 a          979 c          992 g         1532 t
ORIGIN      
        1 tgatttaaat ttaagttgca taatagtaag ctcctgacaa aaattcgaaa tcctacaccc
       61 tgtgatttta gactattacc cttgttctgt ataggttttt aatagcgttt ttcccttttt
      121 tgctctgttt ttttcaaaag tgaactagcg cacaaagctt gctgtttttt agggattttg
      181 ccgccgtggt cacgtggatc tatcgattca acttctacgc gagcggtgcc ttttccgagt
      241 accccgagtt tagctgccgc gacataagat agatcaataa gcctattatc atgaaaggga
      301 ccatgatcgt ttactttaac gataacaact tttccatttt ctaaatttgt gacctttaca
      361 taggtaggaa gagggagagt ggggtgtgca gctgtcatgg caaacatatt atagggttcg
      421 ccactagatg ttttacgacc atgaaattgt gtaccatacc aagaagcgtg tccacgggca
      481 cgatagcctc tgctggttgg cataacgcta tatcgctttc ttaaagcaac gtaagtattt
      541 gttttgccct ttttaaaacg attgccatag cggcttaagg gttcaacttt gggtaccgca
      601 tcgggggttc ttaaaagatt tcgtggaata tgtttaggag gaccatcttt ttttttctta
      661 acacgcgtgc tacaacctga caatgttatt gtgacgcaga taagtgtaag cagaagtaat
      721 ttccaaaagg cagtgtgttt gtatttaatc attatttttt gcccttgttt ttagtattcg
      781 aattttttgg cttagttgat atactgccat agcataatgg gtgcttcgat tatatcgcag
      841 aatgacatta aaattatcaa gtccaatcca ttcttctttg tcatgatggg catttttttc
      901 gagtacaatc aatttgcctt tctgtaaggg atgggtcttt tcttgcaggc gcagcgtagc
      961 aataattttt tcattttttt tccatccatt ttttttcatg taattgccta cactcccgat
     1021 tacgtctgcc atgtcatgga tcaagtctct ctttcctgtg ccagaaaaat caacagcata
     1081 tcgccgataa ctacttggca taaattgtgg cgttccgatt gcacctgcga aagaaccacg
     1141 ggttgtaacg gggtcaagtg aaccttcatt cactagcaat aaaaaatttt ctaattcacc
     1201 acgaaaaaaa gctgcacggc gtgggtagtc aaaggccaga gttgctaaag cttgtaacac
     1261 gggataatta cctgtgattt tgccataaga agtttcgatc cctaagattg caacaataat
     1321 ttctgaaggc acgccaaatt ttgcttccgc tttttttaat aagtctttat gttctctcca
     1381 aaattgaacg ccttcttgga ttcgtttttc tgtgataacc gtatctcgat atcgatacca
     1441 aggcaacgct tcatattgtt tagacatcag acgcaacact ttgttttccg atccaaattc
     1501 actaaaaatt gcatttaagt ttttttcgtt aagaccatgt tttttgtgca tgctttgaat
     1561 aaattgttgt acctcttttc tcttggcgaa agaggtactt ttattttgct gaatcgtttt
     1621 agcgggtaag gctgctgcat gagcagaaat tagcgtgaat atacaagtga agagaaatac
     1681 ttgtaaaaat ctgaatttgt ttgatagcac tgctaatctc ctattcttaa tatctgatca
     1741 aaaataactt attttgccaa taaagttcga tgggtatgaa tagacatcaa tataccaaaa
     1801 cttgccatga gcgttaccag ggatgtgcct ccataactta ctaagggcag tgggatccca
     1861 acgacgggca atatacccgt taccatgcct atattaacaa acacataaac aaataatgtc
     1921 atcatgaggc tgcctgccaa taatcttcca aacgtatttt gtgcttgtac gctaatatag
     1981 attcctcgga tgataatgta taagtacaaa agtagcaaga caagacatcc aaccaaccca
     2041 aattcttcgc taaatacggc aaaaataaag tcagttgtgc gttcgggtaa aaattctaaa
     2101 ttggattgag tgccatttaa ccagcctttt ccataaagac cgccagaacc aatggcaatt
     2161 tttgattgaa taatttgata tccgctgcct aaagggtcgc gttctggatc cagtaacact
     2221 aatatgcgtt gacgttggta atcgcgcata aaaaaccaca gtgttggtag acacagtgtg
     2281 acgaggctcg cgagagaaaa cagtattttc caggagagtc ccgaaaaaaa gataactaat
     2341 aatccactgc ttaaaatcaa taatgcggta ccgaggtcag gttgtttagc gatcagcagt
     2401 gttggaacga gaattaagac taaagggatg aggagcatag aaactttagg tggcaaacta
     2461 tggtcattaa aatattttgc gatcatcagt ggaaccgcca gtttcattaa ctcagcaggt
     2521 tgaaagcgca caaaccataa atcgagccat cgttgtccgc ctttgctaac gtggcccatc
     2581 accaaaaccg atatcaacat cattaatgca ataccgtata accaaggtgc aatgctgcta
     2641 aaaaatcggg gtggaatttg cgcaataaag agcataatgc cgcaagcaag catgaggcgt
     2701 aatccttgtt tttgaatgac atccatttct ggttttgcac tatacaaaat aagaatgccc
     2761 agagcgctaa gcaagataat gccgatcagc aagggccagt cgaggtgaag tctagatata
     2821 aaactgcggt tactccgaaa cattttcttc tcccgaattt tcctctcctc ctacctcgct
     2881 ttcatcatct gcatcatcaa ttttatctga agttgccata gcagatttgg aatgaaagta
     2941 actatcgagc acaatacgtg ccacgtctgc acctgtcttc ttatatttat tttcaactaa
     3001 aaccgctaag gctatttgag gtttttcgac aggtgcgaat gcaataaacc aggaatgatc
     3061 tcttaaatat gctttaattt tgttcacttc atatttctca tgttgcttta aattaaaaac
     3121 ctggctcgta ccggttttac cggcaaccgt gtacgtgagt ccatggctaa tgcgattagc
     3181 ggttccacca ggttgtacta ctttttgcat gcccttaata ataatgtccc aatttgcatc
     3241 tttttttaaa gtaattttcg gtattgcttt agtattttgt tttatgggtt tttgggtttc
     3301 atcaacacca attgccattg caaaacgtgg ttcaaagccc tgaccacgat tcgccaggat
     3361 tgcggctact tgcgccattt gtaacggtgt tgcaagcaat agtccctggc caataccaat
     3421 attcaatgta tcgcctggat accaatcatc tttatagaca cgttttttcc aatccgcact
     3481 gggtgcaagc ccagaggcct caccggtggt atcaatatga gtgatttttc caagcccaaa
     3541 acgtgtaaaa atattatgaa gatgcttaac gcctaatttg tgtgccagcg taaaaaaata
     3601 agtgtcgcag ctttgtgcta tggctttttc catatcgaca gtgccatgtc catgacgtct
     3661 tgcaaaataa atccaatctc ggtacatgcg tcctacaccg tttaatttat accagcccgg
     3721 atcaaatatt tgaaactccg gggtaacagt ccctaactca agtccttgca atgccaccat
     3781 gggtttaacg gttgaaccgg gtgggtattg tccgcgaata gcacgattaa ataaaggcct
     3841 atcgggagag ttttgtaatg cagcataagt ttgcacatcg atgccttgtg taaagagatt
     3901 gggatcaaag gcaggattgc tgacaagtgc gagtacttcg ccattatttg ggtcaatagc
     3961 aacaatggcg ccttttaaat ctttaagcgc ttccgttgca gcgtgttgta aatcgttatc
     4021 taaagataaa tataaatctt ttccagagac gggtggaata cggtttaata cccgaatcgt
     4081 gcgacctttt gcatcggttt caatatgttg atagccggtt gtgccatgaa gcacatcctc
     4141 gtagaacttc tctacgccaa tttttccgat attgtaagtg ccacgataat ttgcggtatt
     4201 aatttgcgtg agatcttttt cactgatagg ccctatatag cctaaagcgt gcgctaaact
     4261 ttctctaaag gggtaatgac gaattaagcg tgcaacaata tcgacaccag gcacacggtg
     4321 tttctcaacg gaaaatttcg caacttcttc ctctgataat ttgatgcgaa tggggatgct
     4381 ttcatttcgc cgtttgtatt ttaattgttt ataaaattgc tgttgctcgg tgtcggtgag
     4441 tggcataatg tcgttaatga ttttcaatgt atcatcgatg ttgggggttc gttcaggtgt
     4501 taattcgagg ctaaaggcag gcatattttc ggcgagcaaa acaccattgc gatcaaagat
     4561 aagcccgcgg gttggggtaa taggtacaat gcgcacttgg ttatttaaag aaagtgtttt
     4621 gtacacgtca tgttgagata tttgtaagta aactaaacga aaagttaaag ttgtcaaaac
     4681 aataaaaaca agaacagcgg cgatgatcgc gcgattgaga tagagctgac tttctttaaa
     4741 gtggtctttt agggtatgta tatttcgcaa atcatgcacc atcttcacac gggataagac
     4801 gataccatca tgccaaaatt gtatgggttt ctcaatggta atcgagaaat gtcagctaga
     4861 ttccttaaat taatagcaag ctgcttttac atctacatcc ttttgct
//
LOCUS       DAORVT010000048         4888 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_12312_length_4888_cov_3.334575, whole genome shotgun sequence.
ACCESSION   DAORVT010000048 DAORVT010000000
VERSION     DAORVT010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12312_length_4888_cov_3.334575"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="PK389_01555"
     CDS             complement(<1..579)
                     /locus_tag="PK389_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HQY22431.1"
                     /translation="MQIKTLNFEQAKILIIGDVMLDQYWHGDTSRISPESPVPVVHVN
                     RVVERPGGAANVALGVAALGAHATLLGLVGKDAAGSALSNALTAAGVKQLLEILPEHA
                     TITKLRVLSRHQQMIRLDTESPFLESSAKTALHDQYIRILEDVDLVILSDYGKGTLSE
                     SSALIAKARAKNIPVIVDPKSHDYSVYRGASII"
     gene            complement(569..1402)
                     /locus_tag="PK389_01560"
     CDS             complement(569..1402)
                     /locus_tag="PK389_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006035793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQY22432.1"
                     /translation="MNKPLSFKVSVIVTTYNWSHALRAVLDALVAQKTTHPFEIIVAD
                     DGSGAETAGVIRTLKNRVSIPILHIWHTNEGFRAAAIRNKAILAAEGDYIIFLDGDCI
                     PRLNFITRHLELLEAKTFIVGNRVLLNRAFTVSALAEMLPLHRWPLWRWFMAYLKGDC
                     NRVLPFLTQPFMCLRLKTKSRWKGAKGCNLGMWKQDLLNINGWEENFTGWGYEDSDLV
                     IRLLRSGVQRKNGRFYIPVIHLWHPENDRTQERHNWTKLKARETDTQINAEKGLSQYA
                     N"
     gene            complement(1395..2639)
                     /locus_tag="PK389_01565"
     CDS             complement(1395..2639)
                     /locus_tag="PK389_01565"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HQY22433.1"
                     /translation="MSAILEAHIESNKINAFQNRCDLFSKQCAVLLGFFVPVSTFATQ
                     VVLFLLLASWFLAGSLKEKGQFIVNHPVARITFLFFGVFVIGTLYSQASWVDSLWMLE
                     KIGKLLYLPFLLPIMQEKKWRRAGMLAFLSAMLLTLILSLLKIYAGLPIVINPRFSGA
                     AIFKDTIFTNLMMAFASFMVGHLFLNQTHFLKRIGLGILLATLVFYILFMGTGRSGYV
                     VFSVLWLLFALQRSSLKGVAIGFLSLAILLSFAYFNSPVFQTRCLDVLADFDQHTAGN
                     SDNSIGQRLEFLNHTWQLSKERLWFGHGTGSFKEIYQNYSAMNHLTRTQNPHNEYINT
                     LFQLGLFGVTVFVGFFVVLFKWSFALPRSEKWFAQGIILAIATGCLANSWLMDFTSGY
                     FFVMILVFCFGALNLKKDFQCE"
     gene            complement(2649..3698)
                     /gene="waaC"
                     /locus_tag="PK389_01570"
     CDS             complement(2649..3698)
                     /gene="waaC"
                     /locus_tag="PK389_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010450381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HQY22434.1"
                     /translation="MKNILIIKTSSLGDIIHTLPALSDAKKYYPEASFEWVVEDAFQE
                     VPLWHPAVKRIIPVALRRWRKHSWNALKNGEIQTFIKGLRQQKYDAIIDAQGLLKSVS
                     LAVLARGGDRIGFSFRSAREGLSSLFYQKRVVVPWGEHAVTRARALFAKGLEYSLPDT
                     VADYGIDLSRLPALETQNPYYVFLHGTTWSTKHWPEAYWIALAKSIVDAGFGVQLLWG
                     NATERVRANRIQSAVPKILVASRKHTLSEITAILARARGIVTVDTGLGHLAAALGVPT
                     VSLYGPSDPTQSGTLGLNQIHLAADFKCAPCFKRICTYTGEKRVEPACFATVTPEKVL
                     RSLIVGVDRCVDPNC"
     gene            complement(3699..3896)
                     /locus_tag="PK389_01575"
     CDS             complement(3699..3896)
                     /locus_tag="PK389_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007144507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HQY22435.1"
                     /translation="MDKKAKDFNNIGQHVVLHADLPLSCPMPKMASWDAHPRVYLSIL
                     ETGEATCPYCGALYRLENGES"
     gene            4139..4804
                     /locus_tag="PK389_01580"
     CDS             4139..4804
                     /locus_tag="PK389_01580"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HQY22436.1"
                     /translation="MAKHRKGQHAHRISPNSRRSKSNASGPWRWLMMGMLIGILGITA
                     IFLLVTGKSLDKFNTLFNSNPLIRQSNTTPLKSTNQKNSRKPKSTEARHSKIHAPQKE
                     STRFEFYQLLPGMEVPLPEATTETASNTAKPTLAKTEKKLAAAQYLVQAGNFRTLVNA
                     ESLKERLHLQGLSAKIQKIDAEDGEWFRVTLGPFPSETIALSHKSRLAKEKIPGILIL
                     QRQ"
BASE COUNT         1582 a         1171 c          876 g         1259 t
ORIGIN      
        1 gataatactc gcgccccgat acacagaata atcatgcgat ttaggatcga caataaccgg
       61 aatatttttt gcgcgtgctt ttgcaattaa cgctgaagat tctgataacg tccccttgcc
      121 ataatcagag aggatcacca gatcgacatc ctctaagata cgaatatatt gatcatgcag
      181 tgctgtttta gcactcgatt ctaaaaaggg actttctgta tcgagtcgaa tcatttgttg
      241 atggcgactt aaaacacgca attttgtaat agttgcatgt tctggcaata tttctaatag
      301 ctgtttgaca cctgctgctg ttaatgcatt cgataaagca ctcccagcgg catctttacc
      361 cacaagcccc aacaaagtgg catgggcgcc caatgcagca acgcctaaag caacgtttgc
      421 ggcaccacct gggcgctcga ccacacgatt cacatgtaca accggcacag gcgattctgg
      481 tgaaatacgc gaagtatcac catgccaata ttgatctaac atgacatcac caataatcaa
      541 tatttttgct tgttcaaaat tgagtgtttt aatttgcata ttgacttaac cctttttctg
      601 catttatttg tgtatctgtt tctctggctt ttaacttagt ccaattgtga cgttcctggg
      661 ttctatcatt ttcagggtgc cacaagtgaa taaccggaat ataaaaacga ccatttttac
      721 gttgcacgcc agagcgcaat agacgaatca caagatcaga atcttcatat ccccatcctg
      781 taaaattttc ttcccagcca ttaatgttca acaaatcctg cttccacatc ccgagattac
      841 accctttagc acctttccaa cgcgattttg tctttaaacg tagacacata aaaggctgcg
      901 ttaaaaacgg caacacgcga ttacaatctc ccttaagata tgccataaac caacgccata
      961 aaggccagcg atgtaaaggc aacatttctg caagtgctga taccgtgaaa gcacgattta
     1021 ataaaacgcg attgccaaca ataaacgtct tcgcttccaa taattccaaa tgacgtgtta
     1081 taaaatttaa tcgagggata caatcgccat ctaaaaaaat aatataatcg ccttctgctg
     1141 ccaaaatggc tttatttcta atagccgcag cacgaaagcc ttcgttggtg tgccaaatgt
     1201 gtaaaattgg aatagacaca cgattcttca gcgttcgaat cacccctgca gtttcagctc
     1261 ccgaaccatc atcggccaca ataatttcga aaggatgcgt tgttttttgt gcaaccaagg
     1321 catccaacac cgccctaagc gcatgagacc aattataagt tgtgacaata acagaaacct
     1381 tgaaggataa tggcttattc acattgaaaa tcctttttta agtttaaagc accaaaacaa
     1441 aacacaagga tcatcacaaa aaaataacca gacgtaaaat ccatcaacca ggaatttgcc
     1501 aaacaccctg tcgctattgc caagatgata ccttgcgcaa accatttttc tgaacgcggt
     1561 aatgcgaaac tccatttaaa caagacaaca aaaaacccaa caaaaactgt tacaccaaag
     1621 agccctaatt gaaataaggt gttgatgtat tcattatgtg ggttttgagt gcgtgttaag
     1681 tgattcatcg ctgaataatt ttggtagatc tctttaaaac tgcctgtgcc atgcccaaac
     1741 caaagccgct cttttgataa ttgccacgtg tgatttaaaa actccaagcg ttgcccgata
     1801 gaattatccg aattccctgc tgtatgctga tcgaaatctg ctaaaacatc taaacatctt
     1861 gtctgaaaaa caggcgaatt aaagtacgca aaactcaaca atattgccaa actcaaaaag
     1921 ccaatcgcaa cacctttaag acttgaacgc tgtaaagcaa agagcagcca caaaactgaa
     1981 aaaacgacat aaccagaacg ccctgtgccc ataaaaagta tgtaaaacac taaagttgcg
     2041 agtaaaatgc ccagcccaat gcgttttaaa aaatgtgttt gatttaaaaa aagatgccct
     2101 accataaaac tagcaaaagc catcattaaa tttgtaaaga ttgtatcttt aaatattgcg
     2161 gcccctgaaa aacgcggatt tatcacaatc ggtaatccag catagatttt caacaggctt
     2221 aaaattaaag tcaacaacat tgcgcttaaa aaagcaagca ttccggcacg acgccacttt
     2281 ttttcttgca tgatcggcaa caaaaatgga agatataata acttaccgat cttttctaac
     2341 atccacaaac tgtctaccca tgatgcttgc gagtataaag tgcctatgac aaagacgcca
     2401 aagaacaaga atgtgatccg agcaacaggg tggtttacga taaactgacc tttttctttt
     2461 aagctccccg ctaaaaacca agaagccagc agtaaaaata aaaccacttg cgtcgcaaac
     2521 gttgaaacag gtacaaaaaa tcctaataat accgcacact gtttgctaaa tagatcgcac
     2581 ctgttttgaa aagcgtttat tttattagat tctatatgcg cttcaagtat tgcactcatc
     2641 tctatctcct aacaattagg gtcgacacag cgatcgaccc ctacaattaa agaacgcaac
     2701 actttttcag gcgtaaccgt tgcaaaacaa gcaggttcca ctcgcttttc tccggtatag
     2761 gtacaaatcc gcttaaaaca aggtgcacac ttaaaatctg ctgctaaatg aatttgattt
     2821 aaccccagcg tccccgattg cgtaggatcc gatggcccat aaagcgacac ggtcggcaca
     2881 cctaaagcag ccgccaaatg acccaagcct gtatcaaccg ttacgatacc tcgcgcgcgt
     2941 gctaatatcg cagtaatttc acttaaggta tgttttctgg acgcgacaag aatttttggc
     3001 acagcgctct gaatccgatt tgcacgtacg cgttccgttg cattacccca taataattga
     3061 accccaaatc ctgcatccac aatacttttt gctaatgcaa tccaataggc ttcgggccaa
     3121 tgttttgtgc tccaagtcgt gccatgcaaa aaaacataat acggattttg cgtttccaaa
     3181 gccggcaaac gcgacaaatc aataccataa tcggccactg tgtcgggaag gctatactcc
     3241 aaaccttttg caaacagtgc tctcgcacgt gtcaccgcat gttctcccca tggaacaacg
     3301 acacgtttct gataaaataa actcgaaaga ccctcgcgcg cagatcgaaa agagaatcca
     3361 atacgatcac cacctcgcgc taaaaccgcc aatgaaacgc ttttcagcaa tccttgcgca
     3421 tctataatgg catcatattt ttgttggcgt aagcctttaa taaatgtttg aatttcgcca
     3481 ttttttaggg cattccaact gtgcttacgc caacgtcgta gcgcaaccgg aataatacgt
     3541 ttaacagcag gatgccaaag aggcacctct tgaaaagcat cttcgacgac ccactcaaaa
     3601 gacgcttctg gataatattt tttagcgtca ctgagcgcgg gcaacgtgtg aataatatcg
     3661 ccaagtgatg atgtctttat aattaatatg tttttcaact aactctcgcc attttctaaa
     3721 cgatacagtg cgccgcaata aggacaagtc gcctcgccgg tctccaaaat ggagagatag
     3781 acccgagggt gcgcatccca agacgccatt ttgggcatcg ggcaagacaa gggtaagtcc
     3841 gcgtgtaaaa cgacatgctg acctatgtta ttaaaatcct tagctttttt atccatacgg
     3901 cagctccccc ctgtatcacc ttaagcaact gacctattct accataaatc ttagggcgaa
     3961 ggcctctaaa atatcgcttt tcagataatc attttgattc gctgctgaac tcgctttgcg
     4021 gaaactcgca tccgctcact aaaataatta tctgcaaggc tataacgctg gaacatctgt
     4081 tacaataaag aaacctggaa attttgaaaa cacttagagt tatttaaaaa acttttatat
     4141 ggcaaaacat cgcaaaggcc aacacgcaca tcggatatct cctaacagca gacgttcaaa
     4201 atcgaatgcc tcgggtcctt ggcgctggtt aatgatgggc atgttgatag gcatcctcgg
     4261 cattactgcc atctttctat tggtgacagg caaatcattg gataaattca acaccctgtt
     4321 caactctaac ccattaatcc gacaatcgaa caccactccg ctcaaaagca cgaaccaaaa
     4381 aaattcgcgc aaacccaaat ccacagaagc gcgtcattca aaaatccatg cgcctcaaaa
     4441 agaatctact cgttttgaat tttatcaact gctgcccggt atggaggtgc ccttaccaga
     4501 agcgacaact gaaacggcca gcaatacggc taaacccacg ctggctaaaa cagaaaaaaa
     4561 actggcagca gcgcaatact tagtgcaagc aggcaacttt cgtacactgg tgaatgcgga
     4621 gtccttaaaa gaaagattac atttacaagg gctctctgcc aaaatccaaa aaatagacgc
     4681 tgaagatggc gagtggttta gggtcacact cggaccattt ccttcagaaa caatcgcatt
     4741 aagccataaa agccgtcttg ccaaagaaaa aatcccaggc attttaatat tacaacgtca
     4801 atagtctaat taataaggag gcaaaccact tggaacagtt tagaggtaca acgatccttt
     4861 gtgtacggcg aaataatcac gttgttat
//
LOCUS       DAORVT010000049         4778 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_12820_length_4778_cov_3.750159, whole genome shotgun sequence.
ACCESSION   DAORVT010000049 DAORVT010000000
VERSION     DAORVT010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12820_length_4778_cov_3.750159"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..1454
                     /gene="ffh"
                     /locus_tag="PK389_01585"
     CDS             84..1454
                     /gene="ffh"
                     /locus_tag="PK389_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HQY22437.1"
                     /translation="MFESLAERLTRAFKHLSGGARLKEEHITATLKEVRKALLEADVA
                     LSVVKNFIHAVQIKAIGQTVNTQLSPQQHFLKLVNDELVHVMGDANVGLQLNAVPPVV
                     ILMAGLQGSGKTTTIAKLAKYLKESQRKSVAVVSADIYRPAAIDQLRTLATEVGTLFI
                     ESDPSEKPVDIVERGILQAKKQQQDVLIIDTAGRLHIDTAMMDEIKMLARVANPTEIL
                     FVVDSMTGQDAVNTARVFNEALPLTGVILTKTDGDARGGAALSIRQVTGKPIKFIGVG
                     EKISALEPFHPERMASRILGMGDVLSLIEELDAKVDKEKAEKLAKKLQKGHGFDLNDL
                     RDQIKQMLAMGGMASIMDKLPGMGALPQNIKAKVNDKEIIRSLAVLDSMTVHERRRPT
                     VIANSASRIRRVAAGAGSDVQAVRKVLKQHAEMEKMMSKMSAKGGMARMMSSLKGRLP
                     PGMMPR"
     gene            1720..2094
                     /gene="rpsL"
                     /locus_tag="PK389_01590"
     CDS             1720..2094
                     /gene="rpsL"
                     /locus_tag="PK389_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HQY22438.1"
                     /translation="MSTVNQLVRKGRYRAKERTKVPALAGCPQRRGVCSRVYTQTPKK
                     PNSALRKVCRVRLTNGSEVTCYIIGEGHNLQEHSVVLIRGGRVKDLPGVRYKVVRGHL
                     DTSGVKDRKQSRSKYGAKRAKG"
     gene            2101..2589
                     /gene="rpsG"
                     /locus_tag="PK389_01595"
     CDS             2101..2589
                     /gene="rpsG"
                     /locus_tag="PK389_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003369032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HQY22439.1"
                     /translation="MPRKSIVTRRRIIADPVYGSQLLAKFINHIMTRGKKSVAEQIVY
                     GAMNMIESRLHRDPLAVLEKALDHIQPMVEVKSRRVGGATYQVPIEVNAERAMALAMR
                     WLVKFSRERGEKGMMHRLAGEIMDAADEEGGGHGKGGAVKKREDTHRMAEANKAFSHY
                     RW"
     gene            2602..4704
                     /gene="fusA"
                     /locus_tag="PK389_01600"
     CDS             2602..4704
                     /gene="fusA"
                     /locus_tag="PK389_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HQY22440.1"
                     /translation="MKRTIPIEQYRNIGIIAHIDAGKTTTTERILYYTGASHKIGEVH
                     DGAAVTDWMVQERERGITITSAAITCYWKGMGAQYAQHRINIIDTPGHVDFTIEVERS
                     LRVLDGACVVFSAVDGVEPQSETVWRQANKYSVPRIAFINKMDRIGADVFRVLEQMKK
                     RLGANPVLMQIPIGSEEHFEGVVDLVRMKAIYWDDSSQGMTFELRDIAPDMLAECEAW
                     REKLVEAASDASEELTDKYIGGEQLTEEEIRAALRIRTLKSEIVPVLCGSAFKNKGVQ
                     AMLDAVIDYLPAPIDVPPIQGILHDKDNTPGFRAPSDTEPFSALAFKVMTDPHVGTLT
                     FFRVYSGVLKSGDTVLNTVRDKRERIGRILLMRANDREEIKEVRAGEIAAAVGLKNTS
                     TGDTLSDPDQLITLERMEFPEPVISVAVEPKTKADQEKMGVALSKLAQEDPSFRVRTD
                     EETVQTIISGMGELHLDIIVDRMRREFKVEANIGKPQVSYRETIRKAVEQEGKYIRQT
                     GGRGQYGHVWLKIEPGALGSGVQFVNEIVGGTVPKEYIPAIEKGVREQAENGALAGYP
                     VVDVKIILFDGSYHDVDSNEMAFKIAGSQGFKAGALKASPVLLEPIMKVEVITPEEYM
                     GDVVGDLSRRRGTILGMDESVSGKHISSEVPLSEMFGYVTDLRSMSQGRATSTMEFLK
                     YAETPASIAQAIVEKRAG"
BASE COUNT         1383 a          868 c         1233 g         1294 t
ORIGIN      
        1 atttgagtat aatcttcgca tgaagaaccg tgtaagacaa ctacgctcga tatagacgat
       61 cgattataga ggataaaaca caaatgtttg aaagtttagc tgaacgccta acgcgtgcct
      121 ttaagcactt aagcggtggg gcacgcctaa aagaagagca tattaccgct actttgaaag
      181 aggttagaaa agcattatta gaagcggatg ttgctttaag tgttgtgaaa aattttatac
      241 acgcggtaca aataaaagcg attggtcaga cagtcaacac acagctttcg cctcagcaac
      301 actttttaaa gcttgtgaat gacgagttgg tacacgttat gggtgatgcc aatgtgggac
      361 ttcaactgaa tgcagttccg cccgttgtta ttttaatggc agggctacag gggtcgggga
      421 aaaccacgac cattgctaaa ttggccaaat acttgaagga aagccaacgc aaatctgtgg
      481 ccgtggtgag cgccgatatt tatcgtcctg ctgcgataga tcagttaaga actttggcaa
      541 ccgaagtcgg tacgctgttt atcgagagcg atcccagcga aaaacccgtt gatattgtgg
      601 aacgcggtat tttacaggct aaaaaacagc agcaagatgt tttgattatt gataccgctg
      661 gtcgtttgca tattgataca gcgatgatgg atgagatcaa aatgcttgcc agagtcgcca
      721 acccaaccga aattctattt gtagtggata gcatgacggg tcaagatgct gtgaatacgg
      781 cacgtgtttt taatgaggca ttacccttaa ccggcgttat tttaaccaaa accgatggcg
      841 atgccagagg tggtgctgcg ctatcgattc gccaagttac tggaaaaccg attaaattta
      901 taggtgtggg tgagaaaatc tctgctctgg aaccttttca tcccgaaaga atggcttctc
      961 gtattttggg catgggggat gttttatcgt taattgaaga acttgatgcg aaagtagata
     1021 aagaaaaagc cgaaaaactt gccaaaaaac tccaaaaagg gcatggattt gatctaaacg
     1081 atttgcgcga tcaaataaaa cagatgttag cgatgggtgg catggcgagc attatggata
     1141 aattacccgg catgggtgct ttgccgcaaa acataaaagc aaaagtgaat gataaagaaa
     1201 ttattcgttc gctggcggtg ttggattcga tgacagtgca tgaacgccga cggccaacgg
     1261 tgatcgcgaa ttcagcgtca cgcatacgtc gtgtcgctgc gggtgcaggc agcgatgttc
     1321 aagcagtgag gaaggtgcta aaacagcatg cagaaatgga aaaaatgatg agcaaaatgt
     1381 ctgccaaagg gggcatggca cggatgatga gcagcttgaa gggtcgcttg cccccaggaa
     1441 tgatgccccg gtaacgcccg gtaacgtgtg atcctttttt cttccattaa cgacaaagct
     1501 gtgttatctt aaccgctctt gccaaaaggc aatggtaaaa acaatgggtt gcatatatat
     1561 acggcgtaac cctaaaggat tagtcaaaac cttattgaca tgaggttttt gtacttgtaa
     1621 gatccagact cctcaataaa ggaagtttct ggacttttat tttgttttgt aggtagggct
     1681 ggaccgctgt gtcgcccaga actggagcag ttagaattta tgtcaaccgt taatcaattg
     1741 gttcgcaaag ggcggtatcg cgctaaagag agaacgaaag tacctgcttt ggctgggtgt
     1801 ccgcaacgtc gtggtgtttg ttctcgggta tatactcaaa cgcctaaaaa gccaaactca
     1861 gcattgcgta aggtatgtcg ggtgcgctta accaacggtt ctgaagtgac ctgttacatt
     1921 attggtgaag gtcataattt acaagaacac tcggttgttt tgattcgggg aggccgcgta
     1981 aaggatcttc ccggtgtacg atataaagtt gtccgtgggc acctcgatac gagtggtgtt
     2041 aaagatcgaa aacagagtcg ttctaaatac ggtgcaaaac gcgctaaagg gtagagaaag
     2101 atgccaagaa aatcaattgt tacaagacgc cgcattattg cggatcccgt ttatgggagt
     2161 cagctattag ctaaatttat taatcatatt atgacgcgtg gaaaaaaatc tgttgcggaa
     2221 cagattgttt atggcgcaat gaatatgatt gagtcgcgct tgcatcgcga tccgttggcc
     2281 gtattagaaa aagcattgga tcacattcaa ccgatggtcg aagtaaaatc ccgtcgggtg
     2341 ggtggtgcta cttatcaggt gcctatcgag gttaatgcag aacgcgctat ggctttggct
     2401 atgcgttggt tagtgaaatt ttctagagaa cgcggcgaaa aaggcatgat gcacaggctc
     2461 gctggcgaaa ttatggatgc tgctgatgaa gaaggcggtg gtcatggaaa agggggtgcc
     2521 gttaagaagc gtgaagacac gcatcgaatg gcagaagcaa acaaagcatt ttcacattat
     2581 cgttggtaat ttaagagtat gttgaaacgc actataccta ttgaacaata tcgtaatatt
     2641 ggcattatcg cgcatatcga tgctggcaaa acgacgacta cagagcgtat tttatattat
     2701 acgggtgctt cccataaaat tggtgaagtg cacgatggcg ctgcggtgac agattggatg
     2761 gtgcaagagc gtgagcgggg tattaccatt acttctgctg cgattacctg ctattggaaa
     2821 ggtatgggtg ctcagtatgc acaacatcgt attaatatta ttgacacacc tggacacgtt
     2881 gattttacca ttgaagtgga gcgttcgttg cgtgtgctcg atggtgcgtg cgttgtgttt
     2941 tctgctgtgg acggtgtaga gccacaatct gaaacagttt ggcgtcaagc gaataaatac
     3001 tcggtgccgc gtattgcttt tattaataaa atggatcgta tcggcgcaga tgtatttcgc
     3061 gtgttggaac aaatgaaaaa acgcttaggc gcaaatcccg ttttaatgca aattccgatt
     3121 ggctctgaag aacatttcga aggcgttgtc gatcttgtgc ggatgaaggc aatttattgg
     3181 gatgatagtt ctcaaggtat gacttttgaa cttcgcgata tcgcaccaga tatgcttgcc
     3241 gaatgcgaag cgtggcgtga aaaattagta gaagcggcaa gcgatgcttc cgaagaattg
     3301 acggataaat atattggtgg tgagcaatta acagaagaag aaattcgagc tgcactccgt
     3361 atacgtactt tgaaatcaga aattgtccct gttttatgtg gatctgcctt taaaaataaa
     3421 ggcgtacaag caatgttgga tgccgttatt gattatctac cggcacctat tgatgttcca
     3481 cctatccaag gtatcttgca tgataaagac aatactcctg gattccgcgc gccttctgat
     3541 acagaacctt tttctgcatt agcttttaaa gtgatgacgg atccacatgt gggtactttg
     3601 acttttttcc gagtttattc gggtgttctg aaatcaggtg ataccgtatt aaatacggtt
     3661 agagataaac gtgaacgcat tggtcgaatc ttattgatgc gtgccaatga tcgcgaagaa
     3721 attaaagaag tgcgagcagg agagattgct gcagcggttg gtttgaagaa tacatctact
     3781 ggggatacat taagtgatcc tgatcagtta attactttgg aacgtatgga atttccggaa
     3841 cctgttatat ctgtagccgt tgaaccaaaa acaaaggccg accaagaaaa aatgggcgta
     3901 gcgttaagta aattagcgca ggaagatcct tctttccggg ttcgaactga tgaagagact
     3961 gtgcagacga ttatttctgg tatgggagag ctgcatctcg atattattgt ggatcgcatg
     4021 cgtcgtgaat ttaaagtcga agccaatatt ggtaaaccac aagtttctta tcgtgaaacc
     4081 attcgcaaag cggttgagca agaaggaaaa tacattcgcc aaacaggtgg tcgcggacaa
     4141 tatggccatg tctggttaaa aattgaaccg ggcgctttag gttcgggtgt tcaatttgtg
     4201 aatgaaattg tgggtggtac tgtacctaaa gaatacattc cagccattga aaaaggtgtt
     4261 cgtgaacaag cggaaaatgg tgcgctagcc ggttaccctg ttgtagacgt caagattatt
     4321 ttatttgatg gttcttatca tgatgtcgat tcaaacgaaa tggcgtttaa aatcgcaggt
     4381 tcccaaggtt ttaaagccgg tgcattgaag gcatctccgg ttttattaga gcccattatg
     4441 aaggttgaag taattacacc ggaagaatac atgggtgatg ttgttggcga tctcagccga
     4501 cgacgcggca ctattcttgg gatggatgaa agtgtatcgg gaaaacatat tagttcagaa
     4561 gtgcctttat ctgagatgtt cggatatgta actgatttac gatcgatgtc tcaggggcgt
     4621 gcaacgtcta cgatggaatt tttgaaatat gcagaaacac ctgctagcat tgcgcaagca
     4681 attgttgaaa agcgggcggg ttaattttag gaggatatgg cagtggcaaa aggtaaattt
     4741 gagcgagatc taccccatat taacgtggga accattgg
//
LOCUS       DAORVT010000050         4748 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_12969_length_4748_cov_3.001278, whole genome shotgun sequence.
ACCESSION   DAORVT010000050 DAORVT010000000
VERSION     DAORVT010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12969_length_4748_cov_3.001278"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1629)
                     /gene="nuoL"
                     /locus_tag="PK389_01605"
     CDS             complement(<1..1629)
                     /gene="nuoL"
                     /locus_tag="PK389_01605"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HQY22441.1"
                     /translation="METIKGLSLAVVLFPLGAALIAGLLGRQIGVKGAHSVTIFAVTV
                     SFILSLYLLYVLYSGQSTSFNFKLYTWGRSAHTDFQVGFLIDRLTVLMMSVVTFVAWV
                     VHIYTVGYMYQDPGYQRFFCYISLFTFGMLTLVMSNNFLQLFFGWEMVGLMSYLLIGF
                     WFNKKTAVAANLKAFIVNRIGDFGFILGIAAVFYCFGTLDYAIVFEQVSNIVSMKQAF
                     AFSADITLPAITFICLALFMGAMGKSAQIPLHVWLPDSMEGPTPISALIHAATMVTAG
                     VFMIARLSPLFEYSDVALNFILVIGSLTCLLMGLVGIVENDIKRIIAYSTLSQLGYMM
                     TAMGVSAYPIGIFHLSTHAFFKALLFLGAGSVILALHHEQDIWKMGGLRRKMPITYTT
                     MLVGSLALVGFPLFSGFYSKDLIIEAVKLSHLPAAGFAYYIVLGSVFITALYTFRLFF
                     VVFHTQSRRDKERAHHTQESPAIIWVPLVLLAIPSFIAGAFLVEPLFQNFFQGAIVMH
                     KLHDGFAVLDREFNAMGGGVIGMTSHGLRSLPFCL"
     gene            complement(1648..1953)
                     /gene="nuoK"
                     /locus_tag="PK389_01610"
     CDS             complement(1648..1953)
                     /gene="nuoK"
                     /locus_tag="PK389_01610"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HQY22442.1"
                     /translation="MIALSDYLIVSAILFGIGVAGLFINRKNVIILLLCIELILLSVS
                     TNFIAFSHFLNDMMGQVFVFFILTVAAAESAIGLAILVVMFRQKKTIDVEEMNTLKG"
     gene            complement(1950..2558)
                     /locus_tag="PK389_01615"
     CDS             complement(1950..2558)
                     /locus_tag="PK389_01615"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HQY22443.1"
                     /translation="MQNIDKIQPLIFYAFSTLAIFAASMVVISKNPVRAVLFLVLTFF
                     ATAGLWMLLEAEFLAVTLVLVYVGAVMVLFLFVVMMLDVELTPVREGFARFLPLGLSV
                     ATIVVIGLVFAVGAKQFGIDMVPAPAAHGANYSNIEQLGILLYTRFLYPFELAGVLLL
                     VAIIAAISLTFRGRRGTIAPNPAGQLKVRKEDRVTLVEGVGI"
     gene            complement(2542..3033)
                     /gene="nuoI"
                     /locus_tag="PK389_01620"
     CDS             complement(2542..3033)
                     /gene="nuoI"
                     /locus_tag="PK389_01620"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005220216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HQY22444.1"
                     /translation="MAKRLKQYLKSLLLWELLLGLKLTGRHFFATKVTIQYPEETTPY
                     SPRFRGIHALRRYANGEERCIACKLCEAVCPALAITIEAAPRSDGSRRTTLYEIDLFK
                     CVYCGFCEESCPVDAIVETGISNYHFENRGENILTKEKLLAIGDRYESEIAAGRVQDA
                     EYR"
     gene            complement(3053..4075)
                     /gene="nuoH"
                     /locus_tag="PK389_01625"
     CDS             complement(3053..4075)
                     /gene="nuoH"
                     /locus_tag="PK389_01625"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005769061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HQY22445.1"
                     /translation="MLDQFLTLGWILLKIFAIVLALMLIAAYLSLAERRFIGFMQDRI
                     GPNRVGFYGLLQPIADGVKLLFKEIILPTRSNHYLFMVAPILAIGPAFAAWAVIPFDQ
                     GFVLANINVGLLYLLAMTSLAVYGIVIAGWASNSKYAFLGALRSAAQIISYEIPMGFA
                     LVGVLIAAGSMNLSDIVLAQRGGILCWYWLPLLPLCVIYWISAVAETNRLPFDVAEGE
                     SEIVAGFHVEYSGIAFSIFFLTEYANMTLVSALVTLLFFGGWLSPFEGIPLLAAWFFW
                     VPGIVWLMLKVSFFLFSFLWLRATFPRYRYDQIMRLGWKIFIPITLIWIVVVTIMVKA
                     NVKPWF"
     gene            complement(4068..>4748)
                     /locus_tag="PK389_01630"
     CDS             complement(4068..>4748)
                     /locus_tag="PK389_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQY22446.1"
                     /translation="AGAWLAGCVPHRLPGGETIKSEIGLNAAQMWDKKLKAYLLLNIE
                     PDFDCFNGSKASQALASADCVVAITPFESLALLEVADLLLPMAPFSETAGTFVNCEGK
                     WQSFKAATLPMNDSRPAWKILRVLGNLLDLEDFKYESAEEIAALVKAKTTEMAAPSAN
                     NNHNSPLDLRKAFSSIKPFASRELQNILTSISPVGLYAADGLVRRAKSLQETLRLKVE
                     MELEMKDA"
BASE COUNT         1645 a         1116 c          794 g         1193 t
ORIGIN      
        1 taagcaaaaa ggcagcgaac gaagaccatg cgatgtcatg cctatcacac caccacccat
       61 cgcgttaaat tctctatcca acaccgcaaa tccatcgtgc agtttgtgca taacaatcgc
      121 accttgaaag aaattttgaa acaaaggctc tactaaaaat gcacctgcaa taaaagaagg
      181 aatggctaat aatactaagg gaacccaaat aatggcagga gattcctgtg tatgatgagc
      241 cctttcttta tctcgacgcg attgcgtatg aaatacgaca aaaaataatc taaaggtata
      301 cagtgctgta ataaaaacac tacccaaaac aatataataa gcaaaccctg ctgctggtaa
      361 atgagataat tttaccgctt caataattaa gtctttagaa taaaatcctg aaaataacgg
      421 aaaaccaacc aaggccaggc tacctaccaa catggttgta taggtaatcg gcatttttcg
      481 tctcaaacca cccattttcc aaatatcttg ctcgtgatgc aaagcaagaa taacagaacc
      541 ggctccgaga aataataacg ctttaaagaa tgcatgtgtg cttaagtgga atatcccaat
      601 gggataagcc gaaacgccca ttgccgtcat catgtaccct aactgagaca aagtagagta
      661 tgcgataatc cgtttaatat cgttctcgac aatacccact aaacccatta agagacaggt
      721 caaagatcca atcactaata taaaatttaa ggctacatcg gaatattcaa ataatggcga
      781 caatcgcgct atcataaaga ctccagctgt taccatggtc gcagcatgga tcaaggcaga
      841 aatcggggtt gggccttcca tagaatcggg caaccacaca tgtaagggta tttgcgcaga
      901 tttacccata gcgcccataa acaaggcaag acagataaat gttatggcag gcagcgttat
      961 gtccgctgaa aatgcgaatg cttgcttcat cgacacaata ttagaaactt gttcaaaaac
     1021 aattgcgtaa tctaatgttc caaaacaata gaatacggca gcaataccca atataaaccc
     1081 aaaatcacca atacgattaa caataaacgc ttttaaattc gcagcaacag ccgttttttt
     1141 attaaaccag aatccaatga gcaaatacga cattaagcca accatttccc atccgaaaaa
     1201 taattgtaaa aaattattgc tcatcacaag tgttaacatg ccaaaggtaa aaagtgagat
     1261 ataacaaaaa aatcgctggt aacccggatc ttgatacata tagccaactg tataaatatg
     1321 cacaacccaa gcgacaaagg tcacaacaga catcattaaa actgtcagcc gatcaattaa
     1381 aaatccaact tgaaaatctg tatgtgcact tcgaccccag gtatataact taaaattaaa
     1441 tgaagtgctc tgtccgctat ataaaacata taaaagataa agcgatagga taaaagacac
     1501 agttacagca aaaatagtaa ccgaatgtgc ccctttaacg ccaatctgtc gtcccaataa
     1561 gccagcaata agtgcggctc ccaagggaaa cagtacaaca gctaaactaa gcccttttat
     1621 cgtttccata ttagcctttt tcctgagtta cccttttaaa gtattcattt cttcaacatc
     1681 gatcgtcttt ttctgtctaa acatgacgac caaaatcgca agccctatgg ctgattctgc
     1741 tgcagcaacg gttaaaataa agaaaacaaa aacctgaccc atcatgtcat ttaaaaaatg
     1801 tgaaaacgca ataaaattgg tgctcacaga cagcaatatc aattcaatgc acagcaataa
     1861 aataataacg ttttttcgat taataaaaag tccagccaca ccaataccaa atagaatagc
     1921 actgacgatc agataatctg ataatgcaat catataccca ccccctccac cagcgttact
     1981 ctgtcttctt ttcgtacttt cagctgacct gctggattgg gcgcaatcgt acctcgtctt
     2041 cctctaaagg ttaaactaat tgctgcaata atagcgacta aaagtaaaac acccgccaat
     2101 tcaaatgggt acagaaagcg cgtatacaac agtatcccta actgctcaat attactataa
     2161 tttgcgccat gtgccgctgg tgcaggcacc atatctatac caaattgttt tgcacccaca
     2221 gcaaaaacca acccaatcac cacgatggtc gcaacagaaa gtcccagtgg caaaaatcga
     2281 gcaaaacctt cacgaacagg cgttaattcc acatccaaca tcatcaccac aaacaagaac
     2341 agtaccataa cagcacctac gtatactaac actaaggtta ctgctaaaaa ctccgcttct
     2401 aataacatcc aaagaccggc tgtcgcaaaa aaagtcaata ctaaaaagag taccgctcgt
     2461 accggatttt tagaaataac caccatactg gcggcaaaaa tcgcaagtgt ggaaaatgca
     2521 taaaatatca gaggttgaat tttatcgata ttctgcatct tgtaccctac ctgctgctat
     2581 ttctgattcg taacgatcac caatggccaa caatttctct ttggtcaaaa tattctctcc
     2641 acggttttca aaatgataat ttgaaattcc cgtctctaca atagcatcca ctgggcagga
     2701 ttcttcacaa aaaccacaat acacgcattt aaataaatcg atttcgtata aagttgtacg
     2761 tctagaaccg tcacttctgg gtgctgcctc aatggtaatg gctaatgcag gacaaacggc
     2821 ttcgcacagt ttacaggcaa tgcaccgctc ttcgccattc gcatagcgac gtagggcatg
     2881 gatccctcta aaacgaggag aatagggcgt agtctcttcg ggatactgta tggttacttt
     2941 ggttgcaaaa aaatgcctac ctgttaattt taaacccaat agcaattccc acagtaacaa
     3001 gctttttaaa tattgtttga ggcgttttgc tattttcaag atctatcctc tatcaaaacc
     3061 atggtttaac gttcgctttt accattattg ttacaacgac tatccaaatc aatgtaatag
     3121 gtataaaaat tttccaccct aatcgcataa tttgatcata acgatatcgt ggaaacgttg
     3181 ctcttaacca caaaaacgaa aacaaaaaga aactcacttt taacattagc caaacaatac
     3241 ccggaaccca aaaaaaccat gctgcaagca gcggtattcc ctcaaatggc gatagccatc
     3301 caccaaaaaa gagtaaagta accaatgccg acaccaatgt catgttggca tattctgtta
     3361 agaaaaaaat ggaaaaagca atacctgaat attccacatg aaagcctgca acaatttctg
     3421 attcgccttc tgccacatca aagggcaagc gattggtctc cgcaacagca gaaatccaat
     3481 agatgacaca taatggcaat agcggtaacc aataccaaca gagaatcccg ccacgctgcg
     3541 caagcacgat atccgataaa ttcatgctac ccgcagcgat taaaacaccc accaatgcaa
     3601 accccatggg gatttcataa gagataattt gagctgccga tcgcagagcg cctaaaaacg
     3661 catatttaga attagaggcc catcctgcaa taacaatgcc atatactgcc aaagacgtca
     3721 tagccaataa atataataat ccgacattaa tattggctaa tacaaatccc tgatcaaacg
     3781 gaataactgc ccatgctgcg aaggcgggac caattgctaa tatcggtgca accataaaaa
     3841 gataatgatt cgaacgagtg ggcaaaataa tttctttaaa gagtaatttt acaccatcgg
     3901 caataggctg caacaatccg taaaaaccga cgcgattagg tcctatgcga tcttgcataa
     3961 accctataaa tcgacgctcc gctagcgata aataagccgc aattaacatc agcgccaata
     4021 caatggcaaa tatcttgagt aagatccaac ccaaggttaa aaattgatca agcatctttc
     4081 atctctaatt ccatctctac ttttaaacgc aatgtctctt gcaatgattt tgcccgccga
     4141 actaacccat cggccgcata aagaccaacc ggcgatatcg atgtaaggat attttgcaac
     4201 tctcgcgatg caaatggctt gatgcttgaa aatgcttttc tcaaatctaa aggcgaattg
     4261 tgattattat tggcactcgg tgctgccatt tcagtcgttt ttgcttttac taacgctgca
     4321 atctcctctg cactttcata tttgaaatct tctaaatcca ataagttgcc tagtactctt
     4381 aagattttcc aagccggtcg actatcattc atcggcaagg tagccgcctt aaaagattgc
     4441 cattttcctt cgcaatttac aaatgtaccc gcggtttcgc taaagggtgc catcggcaac
     4501 aaaagatcgg caacttctaa taacgctagg ctttcgaatg gcgttatagc caccacacaa
     4561 tctgcggagg ctaatgcttg actcgcttta gaaccattaa agcaatcaaa atcgggctcg
     4621 atatttaaaa gcaagtaggc ttttaatttt ttatcccaca tttgtgctgc attcaatccg
     4681 atttcgctct tgattgtttc accacccggt aaacgatggg gtacgcatcc tgccaaccat
     4741 gcacctgc
//
LOCUS       DAORVT010000051         4704 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_13198_length_4704_cov_3.555173, whole genome shotgun sequence.
ACCESSION   DAORVT010000051 DAORVT010000000
VERSION     DAORVT010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13198_length_4704_cov_3.555173"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2032
                     /locus_tag="PK389_01635"
     CDS             <1..2032
                     /locus_tag="PK389_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006035698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV secretion protein IcmB"
                     /protein_id="HQY22447.1"
                     /translation="KSTLATILSFAHSNNRLIGNAVELLRSLEVNTDDAIVKLQVTAS
                     TWAKVGDEKLLRLRVAELAKAIQGWGYCETSEISGDPFAGMMSNALGISANSIATPSV
                     APLSDVTFMLPFTRPASLWTTGAVLFRSPDGKPWPYQPGSTQQTTWIDLMYARPGSGK
                     SVLSNALNLALCLSGGIQRLPNIAIVDIGPSSSGLVSLIKEALPKDKQHLVAYHRMRM
                     SSDMAINPFDTQLGSRFPTPQERAFLVNFLTLLATPVGQENAYDGITDMSGMIIDELY
                     KSLTDTGNPRPYTPNLEALVDKVLPELNIAVDSRTTWWEITDALFKTGHTHEAGIAQR
                     YATPLLADAASICRNQTVADLYGSIKAPTGESIIDAFGRMISGAIREYPILSQPTAFD
                     LGEARVVSLDLDEVAKTGGEAADRQTAIMYMLARYILARHYYLTEDVLKDLPELYQAH
                     HKQRILDIREDPKRIVFDEFHRTSKAKAVRDQVIVDMREGRKWKVQVALISQSLEDFD
                     PVMVEFATSVFIMDAGPKQAIERSTKVFGLSDTARLALETRVHGPRAGGATFLAQFST
                     KQGVNTQLITSTIGPIELWAFNTTSEDARIRNALYEKIGASETRKFLASMYPSGSAAR
                     VVEERMGELKDKSGLISDDSSQSVVEELIKELHERYKKSAASAALSVPTSEN"
     gene            complement(2422..>4704)
                     /locus_tag="PK389_01640"
     CDS             complement(2422..>4704)
                     /locus_tag="PK389_01640"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQY22448.1"
                     /translation="DLANITLKMTPEGRVTLGSVASIVVSEKTTFLKIRENGQEIVGL
                     GVIPKPQANPLEVVGRLHAFVKDLKKTMPPSMSAVVSFDATRPFAAAFVEMRHTLWEA
                     IALVGIIVTLSLASVRAALLPMVTVPLCLIGSFALMWVLGFSINPVTLLALVLAVGLV
                     VDDAIVVVENIYRHMEEGLSAFKAALVSMKEISFAVIVMTITLIAVYLPLVFQVDESA
                     ILFREFAWTLAGSVLISGFVALTLTPALGGKFLKHSKKVVFWERLATRYRGWLAIALR
                     HPVKICVLLLVIAGLGAIGFQRLPSELMPVEDEGILTGNINADNTVSELVREDWFKRV
                     EAVLATIPESERILTGVWQDQWMWWTVILKPNTERIRTSFDIAKELRPRLKEIVGPDV
                     QLSDSRNSTGDDSFKIVVQYAGTSQRLVDAVNNIMAETRKLPGFDSIFSQQTFEKPRL
                     SVKIDRALAAELGVSISAIEETLYTFLSGTKAADFNFDGLDYDVQVRAPLEYRSELNS
                     LNAYFVAGAEGQWVPLGSLVTLKEILEPNAIKHEERMRSASIYMSLQPGISLDKAMQT
                     LDPIIKKHLPPDARHRFGGKAEQYRESRNAMWITFGLALAFIYLVLAALFESFIHPFI
                     VLLTVPLSITGAVWAVNFIGGTNNAYTVIGLVTLMGLITKHGILIVDFANRLRTQNYA
                     LMEAVLMAATSRLRPVLMTTFAMICGAIPLIFSEGSGAIARKDIGWVIIGGMLMGTVF
                     SLFVIPVAYSLMARKKTQQL"
BASE COUNT         1383 a         1113 c          911 g         1297 t
ORIGIN      
        1 aaagtcaacg ctggcaacca ttttaagttt tgcacacagc aataatcgct taatcggcaa
       61 tgccgttgaa ttattgcgtt ccttagaagt caacacagat gatgctatcg tcaaactaca
      121 ggttacagca tcaacctggg caaaagttgg ggatgaaaaa ttattgcgtt tacgtgtcgc
      181 agagctcgcc aaagccattc aaggatgggg atattgcgaa acctctgaga tctcgggcga
      241 tccgtttgcg ggtatgatgt ctaatgcatt agggatttct gccaacagca tcgccacccc
      301 ctcggtcgcg cctttatcgg atgttacgtt tatgctgcct tttacacgcc ctgcttctct
      361 ttggacaaca ggcgcagtat tatttcgatc gccagacgga aaaccctggc cctatcaacc
      421 aggttccacc caacaaacca cctggatcga tttaatgtat gcaagaccgg gttcgggtaa
      481 atcggtttta tccaatgctt taaaccttgc cttatgtctc tctggtggta tacaacgctt
      541 accgaatatt gccattgttg atataggccc ctcttcgagt ggtcttgttt ctttaattaa
      601 ggaagcatta cctaaagata aacaacattt agtggcatat catcggatgc gcatgagctc
      661 tgacatggcc attaacccat ttgatacaca actgggttca cgttttccaa ctccccaaga
      721 acgcgccttt ttagtgaatt ttttaacttt actcgcaacc cctgttggtc aagaaaatgc
      781 ttacgatggc atcacagata tgtcgggcat gattatcgat gaactttata agtcgcttac
      841 cgatacagga aatccacgcc cttatactcc taacctggaa gcgttggtag ataaagtgct
      901 accggaatta aatattgctg tcgatagccg taccacatgg tgggaaatta ccgatgccct
      961 gtttaaaaca gggcacacgc acgaggccgg cattgctcaa cgttatgcca ctcccttatt
     1021 ggcagatgcc gcttctattt gtcgtaacca aacagtagct gatctctacg gctctattaa
     1081 agcacctact ggagaatcca ttattgatgc gtttggtcgt atgatttctg gcgctattcg
     1141 cgaatatccc attttatcgc aaccaactgc attcgattta ggcgaagccc gtgttgtgtc
     1201 tttagatctc gatgaggtgg caaaaacagg tggtgaggct gctgatagac aaaccgctat
     1261 tatgtacatg ttggcccgct atattttggc acgtcattat tatctgaccg aagatgtctt
     1321 aaaagactta cccgaattgt atcaggcaca tcataagcaa cgaattttgg atattcgaga
     1381 agatcccaaa cgcattgtgt tcgatgaatt ccatcgtact tctaaggcaa aagccgttcg
     1441 agatcaagtc atcgtcgata tgcgcgaagg tcgaaaatgg aaagtacagg tcgctttaat
     1501 ctcgcaatcc ttagaagatt tcgatcccgt gatggtggaa tttgccactt ctgtgtttat
     1561 tatggatgcg ggccccaaac aagcgattga acgatccaca aaagtttttg gtttatcgga
     1621 taccgcacgg cttgctttag aaacacgtgt tcatgggcct cgtgcaggtg gtgccacttt
     1681 cttagcacaa ttctctacca aacaaggcgt gaacacgcaa ttaattacca gcaccattgg
     1741 tcccatcgaa ctctgggcat ttaacactac ctcagaagac gccagaattc gaaatgcctt
     1801 atacgaaaaa attggtgcat ccgaaacgcg aaaattctta gcaagcatgt acccctcagg
     1861 ctctgctgcc cgcgtggttg aagagcgcat gggagaactc aaagacaaaa gtggcttaat
     1921 ttcagatgat agcagtcaaa gtgttgtgga agaattgatc aaagaacttc atgaacgcta
     1981 taagaaaagc gcggcttcag ccgcgctttc cgtacccact tcagaaaatt aagtaaataa
     2041 tactcttggg ggaagtatgt gaagtggtgc tggttgacga tttgcttcta tcttgcgcag
     2101 caacttgctt ttctcgtaca agaactctag cgtttttctt gtatccgctt gcggatcagt
     2161 cagttgagct cgtatcttgt gatccaatcg atctagcctt gcattctgtt gtgcagtaaa
     2221 catcttcaat ctcctatgtt atttattcaa ttttgcaaca aggaaattat aaccatacac
     2281 atttaaatta aacaaattta tttttagcgt gtatatacaa tattattgat tttttcagtg
     2341 tttttttatg attcactctt taagggtttg taagactctt gacttttctc tgcacgttta
     2401 gaacctaaaa atagccagtt tttatagttg ttgtgttttt tttcttgcca ttaacgaata
     2461 agcaacgggg ataacgaata acgagaacac tgtgcccatt aacataccgc caataataac
     2521 ccagccaata tcttttcggg caatcgcacc ggaaccctcg ctaaaaataa gcgggatagc
     2581 gccacagatc atcgcaaaag ttgtcattaa gacaggtctt agacgggagg ttgcagccat
     2641 taataccgct tccatgagcg catagttttg tgtacgcaat cggttagcaa aatcaacaat
     2701 taaaataccg tgtttagtaa taagtcccat gagcgtaacc aatccaatca cggtataagc
     2761 attgtttgta cccccaataa aattaaccgc ccacacagcg cctgtaatag aaagcggcac
     2821 ggttaataac acaataaagg ggtgaataaa actctcaaat agcgccgcca atactaaata
     2881 gataaaagcc aaggctaatc caaaggttat ccacatggcg tttcgcgatt cacgatattg
     2941 ctctgccttt ccaccaaaac gatgtcgcgc atcgggtggc aaatgttttt tgataatcgg
     3001 atctaaagtt tgcatggctt tatctaaaga aatacccggt tgcaaagaca tataaataga
     3061 agcactacgc atccgttcct catgtttaat cgcattcggt tctaatattt ctttcaatgt
     3121 caccaagcta cctaagggaa cccattgccc ctctgctccc gcgacgaaat aggcatttaa
     3181 actatttaat tccgaacgat attccaaggg tgctctaact tgcacatcgt aatccaagcc
     3241 atcaaaatta aaatcagccg cttttgtgcc tgataaaaaa gtgtataacg tttcttctat
     3301 tgcactaatg ctaacgccca actctgccgc taatgcacga tcaatcttaa cgcttaaacg
     3361 cggcttctca aaagtttgtt ggctaaagat tgaatcaaaa ccgggtaatt ttcgtgtttc
     3421 cgccatgata ttgttcaccg catctactaa tcgctgagaa gtgccagcat actgaacaac
     3481 gattttaaaa gaatcatctc ccgttgaatt ccgactatcg gacagttgca catcaggacc
     3541 cacaatttcc ttaagacgcg gtctcaactc ttttgcaata tcaaaactgg tacgaatccg
     3601 ctccgtgttc ggttttaaaa taactgtcca ccacatccac tgatcttgcc aaacccctgt
     3661 taaaatacgt tcactttcag gtatggtcgc taatacagct tcaactcttt taaaccaatc
     3721 ttctcggacc aattctgaaa cagtattatc tgcattgatg ttcccagtca aaataccttc
     3781 atcttcgact ggcattaatt ccgaaggcaa cctttgaaat ccaatagccc ctaatccggc
     3841 aataacaagc aataatacgc aaattttaac cgggtgtcgt aaggcaatgg ctaaccaacc
     3901 gcgataccgt gttgcaagcc tctcccaaaa aacaactttt ttagaatgct ttaaaaactt
     3961 tccacctaat gccggtgtta gtgttaaagc aacaaaccct gaaattaaga cgcttccggc
     4021 taaagtccag gcaaattcac gaaacaatat ggcggattca tcgacttgaa aaaccaaagg
     4081 taaatagacg gcaatcaagg taattgtcat cacgatgact gcaaaactaa tttctttcat
     4141 gctcaccagt gcggctttaa aagcagacaa cccctcttcc atatgcctat aaatattttc
     4201 aaccaccaca atggcatcat caaccaccaa accgaccgca agtactaatg ccaataatgt
     4261 cacgggatta atactaaacc ccaacaccca catcaatgca aaagatccga ttaaacataa
     4321 gggtaccgtt accatgggca acagcgctgc acgaacggaa gccaaagaga gcgtgacaat
     4381 aatacccact aatgcgatgg cctcccacaa tgtatggcgc atctcaacaa aagcagctgc
     4441 aaaaggtcgg gttgcatcaa aactcacaac tgcgctcatg gagggaggca tggtcttctt
     4501 taaatctttc acaaaagcat gcagccgacc cacaacctct aaagggttag cttgcggctt
     4561 tggaataacg cccaacccga ctatttcttg cccattttcg cgtattttta aaaaggtcgt
     4621 tttttcagac actacaatgg aagccacact gcccagtgtt acacgccctt cgggcgtcat
     4681 ttttaaggta atattcgcaa gatc
//
LOCUS       DAORVT010000052         4638 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_13514_length_4638_cov_3.038621, whole genome shotgun sequence.
ACCESSION   DAORVT010000052 DAORVT010000000
VERSION     DAORVT010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13514_length_4638_cov_3.038621"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PK389_01645"
     CDS             <1..378
                     /locus_tag="PK389_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HQY22449.1"
                     /translation="ETLLPLSQEVGDALLLYLKKARPRVAFNHVFLCLNAPYRCLHFS
                     NSISAIVRAALSHAGITNPPSRGANLLRHSMATTMLRNGATLENVSAVLRHRSLDMTS
                     YYAKVDVNMLMQITQPWPEGAPC"
     gene            372..1286
                     /locus_tag="PK389_01650"
     CDS             372..1286
                     /locus_tag="PK389_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010967848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HQY22450.1"
                     /translation="MLNKTIDRYIKMQQTMGFKYRIQACLLRHFGEFAKKNGDVYVRC
                     KTVIDWARLAPSPAQRRNRLLTVRRFAIAMKAEDDRHEIPAPDIFGRETFKRKIRHIL
                     SSEELKLLLTAASQLKPQKTLRPLTYSTLFALLAAAGIRISEALALNIQDISDDGLII
                     RNTKFRKNRLVPLHPSTQQGIQRYLKYRIRHGGIDPALFISSKGTRLSYSTVISVFLQ
                     LVRSIGLRDKPGDSGVCIHDLRHRFAVRSLEQCSGNRDVVSRHMLALSTYLGHAHLSD
                     TYWYLQATPILMKQIAKKQEVFYRRNQS"
     gene            1283..1807
                     /locus_tag="PK389_01655"
     CDS             1283..1807
                     /locus_tag="PK389_01655"
                     /inference="COORDINATES: protein motif:HMM:NF014898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HQY22451.1"
                     /translation="MTNLANYLSIFLREYLPSERGASPHTCETYAYTFQLLVCFAARQ
                     AKIQPSKLTLEAINASLIVAFLKHLEEERKNTPRTRNARLAAIKAFFRFLEYRLPSSL
                     DQSRRIHAIPSKKMDEKLVDYLIREEMQTLLDAPDLQQASGIRDRAILYLAYSAGLRV
                     SELVNLRLDQLQRI"
     gene            complement(1872..2831)
                     /locus_tag="PK389_01660"
     CDS             complement(1872..2831)
                     /locus_tag="PK389_01660"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HQY22452.1"
                     /translation="MEESRIPFEVIEKDFWVVWILEKLFSLSDLKDHLTFKGGTSLSK
                     VYRIIQRFSEDIDLSIEKEFFGFDKDNDPEKAVSKKKQRAALDNLSTTCSEYVQNDFL
                     MALRFAISEKLQTSEGWHLEIDAADPDGQTLQFEYPSTTAKNGYIRPSVKIELGARSE
                     HWPVGNHKIQSYTKTILKEKITEPEVWVKVLDAKRTFWEKATILHQYAHLPAEKKLPA
                     RISRHFYDFFCLLNSDIKASALAEPTLLDRVATHKSIYFASAWASYGTARKGSLVLSP
                     PLTLHDDLEKDFGLMKDMFFGSIPEWSTILKDIRDFETKFNEK"
     gene            complement(2884..3492)
                     /locus_tag="PK389_01665"
     CDS             complement(2884..3492)
                     /locus_tag="PK389_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006342583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HQY22453.1"
                     /translation="MAESIVYNVKNRIIEHGRGWCFTPKHFLDLDSVTGVRSALSRLE
                     KAKVIRRLRQGLYDYPREHPSLGVLPPKAEEVAKAISEKNGIQVQPSGAYAANLIGLS
                     EQVPGRVVFLTNGPSKKVKIGKQEILFRTTTEKNMYPAGTKVGLVIQAFRNLGKENID
                     NIAQARTRKFLFGTSKSELIKNLKYSPQWIRVLIFKIMDVTI"
     gene            3820..4296
                     /locus_tag="PK389_01670"
     CDS             3820..4296
                     /locus_tag="PK389_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22454.1"
                     /translation="MNLTINTPGIVGHEGHYKSAALTVTAQSLMNKGELFIDALRLHC
                     NIVNRGKIVARNATLKRMPGDAEALTLDNTDGVIDIQKRLILQQGRIKNTRGKFCSET
                     LRGNTTGALNNQHGWIQVRKRARLNLRGGLQNSHGAIVQHLGEDHPRYLRSGGPNQ"
     gene            4293..4484
                     /locus_tag="PK389_01675"
     CDS             4293..4484
                     /locus_tag="PK389_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22455.1"
                     /translation="MIKLTVLQAFHAFYSYILADYESKGDLWIVDGVLSDADTNLKIA
                     CHSGGPTAELKKNYQLGCR"
BASE COUNT         1323 a          930 c          956 g         1429 t
ORIGIN      
        1 gagacgttat taccattgtc acaggaggtt ggtgatgctt tgttactata tttaaaaaaa
       61 gctcgaccaa gagtcgcttt taatcatgtt tttctttgtt taaatgcgcc ttatcgttgc
      121 ttgcattttt ccaatagcat ttctgcaatt gtgcgtgctg cgctttctca cgcagggatt
      181 accaatccgc cttctcgtgg cgcaaattta ttacgacatt caatggcaac gactatgttg
      241 cgaaatggcg ctacgcttga aaacgtttct gccgtattgc gacatcgttc tttagatatg
      301 acaagttatt atgccaaagt ggacgttaac atgttaatgc aaattacaca accatggccg
      361 gagggcgcac catgctaaac aaaacgattg atcgttatat caaaatgcaa caaacaatgg
      421 ggtttaagta taggatacaa gcctgtttac tgcgccattt tggagaattc gctaagaaaa
      481 atggcgatgt ttatgtacgc tgtaaaacag tgattgattg ggcaaggttg gcaccttcac
      541 cagcacaaag gcgtaatcga ttgctaacag ttcgtcgatt tgcaattgct atgaaagctg
      601 aagacgaccg tcatgaaata ccggcccctg atatatttgg acgcgagacg tttaaacgaa
      661 aaatacgcca tattttatca tcggaagaat taaaacttct actgactgct gcttcacagc
      721 ttaaacccca aaaaactctt cggcctctga catattccac cttatttgcc ctattggcag
      781 ccgctggtat tcggattagt gaagcattag cacttaatat tcaagatatt agtgatgatg
      841 gtcttattat tcgtaacaca aaatttcgaa aaaatcgcct tgttcccttg cacccgtcta
      901 cgcaacaagg tattcagcgt tatctcaagt accgtatcag gcacggcgga attgatcccg
      961 ccctttttat ctcgagtaag ggtactaggt tatcttactc tacggtgatt tctgtgtttc
     1021 ttcaattagt gcgatcaatc ggactacgtg ataaaccggg agattcagga gtatgtattc
     1081 atgatctcag acatcgtttt gcagtaaggt cacttgagca atgcagtggt aatcgtgatg
     1141 ttgtatcgcg acacatgctt gcattaagta cttatcttgg acatgctcat ctgtcagaca
     1201 cctactggta tttgcaggct acacccatat tgatgaaaca aatcgctaaa aaacaagagg
     1261 tattttatag gagaaatcag tcatgacaaa tcttgccaat tatcttagta tttttttacg
     1321 ggagtactta cccagtgaaa gaggggctag cccgcacacc tgtgaaacat atgcctatac
     1381 gtttcaatta ttagtgtgtt ttgcggcacg tcaagctaaa atacaacctt caaagttaac
     1441 acttgaggct atcaacgcat ctttgattgt tgctttttta aaacatttag aggaagaacg
     1501 caaaaataca ccgcgcacga gaaatgcgag gcttgctgca ataaaggcat tcttccgttt
     1561 cttggaatac cgattgccat cttctttaga tcaatcacgc cgtatccatg ctattccttc
     1621 taagaaaatg gatgagaaac tggttgatta tttaattcgg gaagaaatgc aaaccttact
     1681 ggatgcccca gatctgcaac aggcttctgg tatacgtgac agggccatat tgtatcttgc
     1741 atactctgcc ggactgcgag tatcagaatt ggttaatcta cgtcttgatc agcttcagcg
     1801 gatctgaaaa gtacagaaat atatctgcgc actgactcaa tgcgatatgc ggaatggcaa
     1861 ttataggttc attatttttc attaaacttt gtttcaaagt ctctaatgtc ctttaatatc
     1921 gtgctccact ccggaatact tccaaaaaac atatccttca tcaaaccaaa gtccttttct
     1981 aagtcatcat gaagcgttaa aggtggcgat agcactaagc tgccttttcg ggccgtgcca
     2041 tagcttgccc aggcagatgc gaaatagatg cttttatgag tcgcaactct atctaaaagt
     2101 gtaggctctg cgagagcact agccttgata tctgaattta acaggcagaa aaagtcgtaa
     2161 aaatgtctgg aaattcgggc tggcagtttt ttctctgccg ggagatgcgc gtattggtgc
     2221 agaatggttg ccttctccca aaaagtacgt ttagcatcaa gaactttaac ccaaacttca
     2281 ggttccgtga tcttctcttt aagtatggtt ttcgtatagc tttgtatttt gtgattaccc
     2341 acaggccaat gttcggagcg agcacctagt tcaatcttta ctgatggacg aatataacca
     2401 tttttagccg tagtacttgg atactcaaat tgtagcgttt gaccgtctgg atctgcagcg
     2461 tctatttcaa ggtgccagcc ttcagaagtc tgcaattttt cagatatagc aaatcttaag
     2521 gccataagaa agtcgttttg aacatactca gaacatgtgg tagaaagatt atctaacgct
     2581 gctcgctgtt ttttctttga cacagctttt tcgggatcat tatctttatc gaatccaaaa
     2641 aattcttttt caattgaaag atcaatatcc tcagaaaacc gctgaataat gcggtaaact
     2701 tttgatagag aagttccacc tttaaatgtc aggtgatcct ttaaatcaga aagtgagaac
     2761 agtttttcta agatccatac aacccaaaag tccttttcga tgacttcaaa tggaattctt
     2821 gactcttcca ctgtgacatc aaaaatggtt cgcgttcagc tacagagagt agatgcaatt
     2881 cagtcatatt gtgacatcca taattttaaa aataagcacc cgaatccatt gcggtgaata
     2941 ttttaagttt ttaataagtt cacttttgct tgtgccaaaa agaaattttc gtgttctggc
     3001 ctgagcaata ttgtcgatat tttcttttcc tagatttcta aatgcctgaa tcactagacc
     3061 aactttagtg ccggcaggat acatattttt ttctgttgtt gttctgaata aaatttcctg
     3121 ctttcctatt ttaacctttt ttgatggtcc gtttgttaaa aaaacaacac gcccagggac
     3181 ctgttccgac aaaccaataa ggtttgctgc atatgcccca gatggctgaa cttgaatccc
     3241 atttttttct gagattgctt ttgcaacttc ttccgctttt ggtggaagca cacctaacga
     3301 tggatgctca cgaggataat catacagccc ttgtctaaga cgtcgaataa ccttggcttt
     3361 ctccaggcgt gatagggcgc ttcgaacgcc agtgacacta tctaggtcaa gaaaatgctt
     3421 aggtgtaaag caccatcctc taccatgctc aataatcctg ttttttacat tatatacaat
     3481 cgattcagcc attttttaga tacctcattg ccacaaaata tagcaacttt ttgtggcaat
     3541 gtctagttta gggaaggtac aaccggtaga gggttgactc agcttgttag ctcagaaggt
     3601 cgtgcaggac tttttttctt aactccattg acactttatc cgttatttag cggatatcta
     3661 ttccgatttc tttttgttta gaatgcatga ggcatgcatg gcttttgcct taaaaattcg
     3721 cttaaaaaaa gtgtcatagc tatatcaacc ccgaaaggcg cattgtcttt taaggtgagc
     3781 gctaggggta atattgagct tttgtctgtg cccttagact tgaatttaac cattaataca
     3841 cctggaattg tcggtcatga aggccattat aaaagcgctg cattaaccgt tacggcacag
     3901 tctttgatga ataaaggcga attgttcatc gatgctctgc gccttcattg taatatcgtt
     3961 aaccggggta aaattgtagc gcgtaacgca acacttaaac gaatgcctgg tgatgcggaa
     4021 gcgctgacat tagataatac agacggcgtt atcgatattc agaaaaggct tatcttacaa
     4081 caaggcagaa taaaaaatac ccgcgggaaa ttctgttctg aaacgctgag aggtaatacg
     4141 actggggcgt taaacaatca acacggctgg atacaggtac gaaaacgtgc gcgcttgaat
     4201 cttcgtggcg gtttgcaaaa cagccatggg gctattgttc aacatttggg ggaagatcac
     4261 cctagatatt tgcgttcagg aggacctaat caatgatcaa gttgacagtg ttacaagcat
     4321 ttcatgcttt ttacagctat attttagcag attatgaatc aaagggtgat ttatggatag
     4381 tagatggcgt tttaagcgat gcagatacga acctgaagat tgcgtgccat tctggaggac
     4441 cgacggctga attaaaaaaa aattatcagt tgggttgtcg atgacattat cggttggatt
     4501 gtaatgcata ttagttgttg tcgccttgaa ttgaaatatg tacgctggta ttggaaacca
     4561 ttatggattc ttgggggggt gctaaccatt atcgatgaaa ttggccgaat cgaagtatgg
     4621 cgttggctcc gaaaattt
//
LOCUS       DAORVT010000053         4635 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_13529_length_4635_cov_3.152838, whole genome shotgun sequence.
ACCESSION   DAORVT010000053 DAORVT010000000
VERSION     DAORVT010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13529_length_4635_cov_3.152838"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /gene="mnmE"
                     /locus_tag="PK389_01680"
     CDS             complement(<1..1074)
                     /gene="mnmE"
                     /locus_tag="PK389_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HQY22456.1"
                     /translation="MEQNDADTIVAEATPRGRGGVGVVRISGSKTTEIAQKILGRLPS
                     VGEAKHLLFLDKNNEGIDRGIALLFKAPHSFTGEDVLELQGHGGPVIMDLLVQAAIDA
                     GARNAEPGEFSLRAYLNNKIDLCQAEAIADLINASSKEAAKSALRSLQGVFSDAIQTL
                     VEKLIKLRMHVEAAIDFPEEEIDFLKDDRICVRLKALMAELETIQEKAFQGSLRSDGA
                     RVVIVGHPNAGKSSLLNALSGTDAAIVTDIPGTTRDVLREQIVIDGLLVQLVDTAGLR
                     VGADPVEKEGIARALKEVSLADHVVLVVDGATTTETQPTLLFSEWIAVFPKNIGMTVL
                     MNKIDKWGAMPSLNKKSDHTVIHL"
     gene            complement(1061..2737)
                     /gene="yidC"
                     /locus_tag="PK389_01685"
     CDS             complement(1061..2737)
                     /gene="yidC"
                     /locus_tag="PK389_01685"
                     /inference="COORDINATES: protein motif:HMM:NF002352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HQY22457.1"
                     /translation="MTTQNIRFLLFALIVLIGMLLYNAWKQESVTIPIISHNKSGTQF
                     ENQTRMDDKEIPEVGQGKSVLEQAPFVSQTYSLDREIQIKTDVLNVKIDKLGGDITQL
                     ILTTYAEDENNFGQGFVLFDNSDKRYYVAQSGLTGEQGPDKRGVGRAKYETHQASYQL
                     EDNQTDLIVDLKTTAGTAVDVIKRFVFHRGSYIVDVEYLIYNKGKEDYKGSFYARLKR
                     KAGKDTSGGFLGVQTFTGAAIHTPETPYKKISFKDIADKPLEESVQGGWAAMVEHYFV
                     SAWVPSSGVTSQYQTNSLEHDIYSVGFVESVLTVGGGEEKSIKAQLYAGPEITEDLGK
                     LAPGLELAVDYGILWPICQPIFWLLKKAFQLTSNWGLAIILTTIIIKALFYKLSASSY
                     RSMGNMRKLQPKLEALKQRFGDDKQKYSQGMMELYKKEKINPLGGCLPVLVQIPVFIA
                     LYYVLLGSVELREAPFYFWITDLSGKDPYYVLPILMGLSMLVQQKLNPAPPDPVQAKV
                     MMLMPVVFTVLFLNFPSGLVLYWFVNNVLSIAQQWFITRRIELSLRHGTK"
     gene            complement(2747..2983)
                     /gene="yidD"
                     /locus_tag="PK389_01690"
     CDS             complement(2747..2983)
                     /gene="yidD"
                     /locus_tag="PK389_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007160132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HQY22458.1"
                     /translation="MARFNRTMAKGMIFIIRFYQYCISPLIGNHCRFYPSCSAYALEA
                     LARFGFLKGFFLATRRLLRCHPGCVGFDDPVPLA"
     gene            complement(2947..3312)
                     /gene="rnpA"
                     /locus_tag="PK389_01695"
     CDS             complement(2947..3312)
                     /gene="rnpA"
                     /locus_tag="PK389_01695"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HQY22459.1"
                     /translation="MQSKKLTFSKNERLQTPLEFQAVFREPIRARSAYFTMLARPNER
                     DGARLGVIVAKRIIRRSVRRNAIRRLIRESFRINQCQLTGLDVIVLIRCALPKEPNAL
                     FLDCLVQQWQDLIAQWQRA"
     gene            complement(3315..3449)
                     /gene="rpmH"
                     /locus_tag="PK389_01700"
     CDS             complement(3315..3449)
                     /gene="rpmH"
                     /locus_tag="PK389_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011253345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HQY22460.1"
                     /translation="MKRTYQPSKICRKRKHGFLVRSRTKGGRLVLKNRRQKGRKKLAA
                     "
     gene            3979..>4635
                     /locus_tag="PK389_01705"
     CDS             3979..>4635
                     /locus_tag="PK389_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22461.1"
                     /translation="MPITKENAKAAIRRFFCPIDQGYPSLTQMCFVETFEGKEGKETA
                     ELYGFTRDSIGKWISEGKNKGRVNNPATGLDFKEIAPSLISGEEIFSLVLAIVQASGH
                     LPAVIEEDEEFADFSKTRDYQKALEHGKTLLNFVVNFGEGVDLQAKIQSLSVRNLNGG
                     AAQIRLLEETLKFKLSDFENYNEMGNELPDDLPPPIEAKVDVLIPPQAVMRFAPMLDP
                     F"
BASE COUNT         1364 a         1026 c          913 g         1332 t
ORIGIN      
        1 aagatggata accgtgtgat ctgatttttt gttcaagctc ggcattgctc cccatttatc
       61 gattttattc atcaaaaccg tcataccgat atttttagga aaaaccgcta tccactctga
      121 aaataatagc gtaggctgtg tttctgttgt cgttgcacca tccactacca acaccacatg
      181 atctgctaaa gaaacttctt taagtgcgcg tgcaatgcct tctttttcca caggatcagc
      241 acccactctt aaacccgcgg tatcaaccaa ttgcaccaac agtccatcga tcacaatctg
      301 ctcacgtaac acatcgcgcg ttgtgcccgg tatatcggtt acaatagcag catctgtacc
      361 cgataacgca ttcaaaagac tggattttcc ggcgtttggg tgaccgacaa tcacaacccg
      421 ggccccgtct gaccttaatg atccttgaaa agctttttcc tgaatcgttt ctaattctgc
      481 cattaatgct ttcaacctca cacaaatgcg atcgtctttt aaaaaatcaa tttcttcttc
      541 tggaaaatcg atagccgcct caacatgcat gcgaagctta attaattttt caaccagtgt
      601 ttgaatagca tccgaaaaca ctccctgcag agatcggagc gcacttttag ccgcttcttt
      661 tgagcttgca ttaatgagat cggcaatggc ctctgcttgg caaagatcaa tcttattgtt
      721 taaataggca cgtaacgaaa actcaccagg ctcggcattc cgtgcgcccg cgtcgatcgc
      781 tgcctgcact aagagatcca taatcacagg tccgccatga ccttgtaact ctaaaacatc
      841 ttcaccggta aaagaatgtg gcgctttaaa taaaagtgct atgccgcgat cgatgccttc
      901 gttattttta tctaaaaata gaagatgttt ggcctcaccg accgagggca aacgccctaa
      961 aattttctgt gctatctctg ttgttttaga gccagaaatg cggacaacac ccacgccacc
     1021 tcggcctctg ggtgttgctt ctgcaacaat agtatcggca tcattttgtt ccatgacgca
     1081 atgagagttc tattcttcgt gtaataaacc attgctgtgc gatagaaagc acattattaa
     1141 caaaccaata taaaaccaat ccagatggaa aatttaaaaa gagcacggta aaaacaaccg
     1201 gcatcagcat catcacttta gcctgcactg gatcgggtgg cgctgggttt agcttttgtt
     1261 gcaccaacat actgagcccc atcaagatag gcagaacata atagggatct ttacctgaca
     1321 gatctgtaat ccaaaaataa aatggtgcct ctcttaactc cacgctaccc agtaatacat
     1381 aatacaacgc aatgaatacc ggaatttgaa cgagcacagg caaacaaccg cctaacggat
     1441 ttattttttc ctttttgtag agttccatca tgccttggct atatttttgc ttatcatcac
     1501 caaatcgttg ttttaaagct tcgagctttg gctgcaattt tcgcatattg cccattgagc
     1561 gataactgct ggcagaaagc ttataaaata aggctttaat gataattgtg gttagaataa
     1621 tcgcaagtcc ccaattgctc gtcaactgaa aagctttctt taacaaccaa aaaatgggct
     1681 gacaaatagg ccacaaaata ccgtaatcga cagctaattc aagacccggc gctaattttc
     1741 cgagatcttc tgtaatttca ggtcctgcat ataattgagc tttaatactt ttttcttctc
     1801 ctccgccaac cgttaataca gactctacaa atccaacact gtaaatatca tgttctagcg
     1861 agttggtttg gtactgactg gttacaccgg atgagggcac ccaagcacta acaaaataat
     1921 gctcaaccat agccgcccaa ccgccctgta cagattcttc taatggtttg tcagcaatat
     1981 ccttaaaact gattttttta tacggtgttt cgggtgtgtg aattgcagcg ccggtaaagg
     2041 tttgtacacc cagaaacccg ccactggtat cttttccggc ttttcgtttt agacgcgcgt
     2101 aaaagctgcc tttataatct tctttgcctt tattatagat aagatattcc acatcgacga
     2161 tatacgaccc gcgatgaaaa acaaagcgct taatgacatc aacagctgtt ccggcggtag
     2221 ttttaagatc aacaattaaa tcggtttgat tgtcttcaag ctgataactc gcctgatgtg
     2281 tctcatattt tgctctaccg accccccgtt tatctggccc ttgttcgcca gttaatccag
     2341 attgtgcaac ataatagcgt ttatccgagt tgtcgaataa aacaaaaccc tgtccaaagt
     2401 tgttctcgtc ttctgcatag gtagttaata ttaattgtgt aatatcaccg cccagtttat
     2461 caatttttac attcaataca tccgttttaa tttgaatttc gcgatcgaga gaatatgttt
     2521 gggaaacaaa aggcgcttgt tcaagaaccg attttccttg tccaacttca gggatctctt
     2581 tgtcatccat tcgggtctga ttttcgaact gtgttcccga tttattgtga gaaataattg
     2641 gtattgtcac agactcttgc ttccaggcgt tataaagcaa catgccgatt aaaacaatca
     2701 gtgcaaatag taaaaaacga atattttggg ttgtcacatc aactccttac gctaagggta
     2761 cgggatcatc aaagcccacg cagcctggat ggcatcgcag cagtcttcgt gttgctaaaa
     2821 aaaagccttt taaaaatcca aatcgagcca atgcttcgag agcatacgca gaacagctcg
     2881 gataaaaacg acagtgatta ccaatcaagg ggcttataca atactgataa aatcgaataa
     2941 taaaaatcat gccctttgcc attgtgcgat taaatcttgc cattgttgta ctaaacaatc
     3001 taaaaagagt gcgttgggct ccttgggtag cgcacaccgt attaacacaa tcacatcgag
     3061 gcccgtcaat tgacattgat ttatcctaaa actttctctg atcaatcgcc gaatcgcatt
     3121 gcgacgtact gagcgacgta taattcgttt agcaacaatc acaccgagcc ttgcgccatc
     3181 acgctcgttt gggcgagcga gcattgtaaa gtatgctgat cttgcacgta ttggctctct
     3241 aaagacagct tgaaattcca gaggcgtttg taagcgttcg tttttagaaa acgttagttt
     3301 tttagattgc aaccttaagc ggccagcttt ttacgaccct tttggcgccg attttttaag
     3361 acaagacgtc cacctttggt tcggcttcta accagaaatc cgtgctttcg cttacggcaa
     3421 attttgctcg gttgatatgt tcttttcatg acggctcccg aaatcacttt gtttagcagt
     3481 aagtcggtca ctttatagga tgttgattcc ggtgtcaaat atcgtatcgc cttgcttcat
     3541 tgggtgtaca gccccaaagc gtactagcga tgtcgcctga tcaatcataa taaaagaata
     3601 agaaagatct taaagcttat tatggtgatt actgagtaca gaatctgttg atatcatcta
     3661 atttctgttt taaatcaaat ggctgcgcac caagaaaccc cttggataat cacagtataa
     3721 cggaccgaat agattgtgga taaagtacat ggctttaaaa ttacccgtaa agcttaagtt
     3781 atccacaggc tatgcggcac cttatcagca ggagatgcac agatttagga attgattgac
     3841 ccgctaatgg ctagcatcgc tagggttaaa aaagaacatc actaaagagt gtgatagcac
     3901 gggttgcgac catatttaat tttatgataa agtctcttta ctaagttagt aaaacaaaat
     3961 atattattgg aggttgacat gcccattaca aaagaaaatg ccaaagccgc gattagacgc
     4021 tttttctgcc caattgacca agggtatcca agcttaacgc aaatgtgctt tgttgagacg
     4081 tttgaaggca aagaaggtaa agagacggca gaattatatg gttttacgcg cgacagtatc
     4141 ggaaagtgga tcagtgaagg taagaataag ggcagagtaa ataaccctgc aactgggtta
     4201 gatttcaaag aaatagcgcc atctttaatt tctggtgagg aaatatttag cttggtttta
     4261 gctatagtcc aggcttcagg ccaccttccg gctgttattg aagaagatga agaatttgca
     4321 gacttttcta aaacccgaga ttatcaaaaa gcgttggagc acggaaaaac acttctaaac
     4381 tttgtggtga acttcggaga gggagtggat ctacaagcaa aaattcaaag cttaagtgtg
     4441 cggaatctga acggcggtgc agcacaaata agattattgg aagagacctt gaaatttaaa
     4501 ctgagcgact ttgaaaatta taatgaaatg ggcaacgaat tgcctgacga tttacccccg
     4561 ccgatagaag caaaagtaga cgtgcttata ccgcctcaag cggtgatgcg ctttgcgcca
     4621 atgctggatc ctttt
//
LOCUS       DAORVT010000054         4618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_13620_length_4618_cov_2.942143, whole genome shotgun sequence.
ACCESSION   DAORVT010000054 DAORVT010000000
VERSION     DAORVT010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13620_length_4618_cov_2.942143"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            276..1241
                     /gene="trxB"
                     /locus_tag="PK389_01710"
     CDS             276..1241
                     /gene="trxB"
                     /locus_tag="PK389_01710"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HQY22462.1"
                     /translation="MQNTKHCRLLILGSGPAGYTAAVYASRANLNPVLITGMEKGGQL
                     TTTTDVDNWPGDIEGLTGPALMDRMEQHAKRFNTEIIFDHIAEVKLSSKPFELIGDSK
                     KRYTADALIIATGARAKYLGIPSEQAFMGKGVSACATCDGFFYKNQGVAVIGGGNTAV
                     EEALYLSNIAGHVTLIHRRDKFKAEKILITQLMKKVETGNITILWNTVLDEILGDETG
                     VTGIRVTEVTSKSQQDIALQGVFIAIGHVPNTSIFESQLNMFNSYITVQGGSAGNATA
                     TNIPGVFAAGDVADHVYRQAITSAGSGCMAALDAEKYLDAMQVAS"
     gene            1294..1509
                     /gene="infA"
                     /locus_tag="PK389_01715"
                     /pseudo
     CDS             1294..1509
                     /gene="infA"
                     /locus_tag="PK389_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
     gene            complement(1563..3173)
                     /locus_tag="PK389_01720"
     CDS             complement(1563..3173)
                     /locus_tag="PK389_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005772589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HQY22463.1"
                     /translation="MSIHIIPPFDKGGPGGFPARCTFAIISHPDAGKTTITEKLLLYG
                     GAIQLAGTVKGRKASKHATSDWMALEKQRGISVTTSVMQFPYKNRIINLLDTPGHEDF
                     SEDTYRTLTAVDSALMVIDAVKGVEPRTIKLLEVCRLRDTPIITFINKLDRDSREPVD
                     LLDEIERILNIRCAPVTWPIGMGKHFKGIYHLEFDSICLYQPGKNERIQAGQVIQGLN
                     NPELDRLIGVESAKEFREELSLVQGASHGFDKDAYLKGELTPVFFGSALNNFGVQELL
                     DAFANYAPSPQSRETETRSVEATEAKFTGFVFKIQANMDPQHRDRIAFLRICSGKYEQ
                     GMKMRHVRLAKEIQISNALTFMAGERDRVMEAWPGDIIGLHNHGTIQIGDTFTQGENL
                     KFIGVPYFAPELFRAVRPKDPLKAKALQKGLIQLSEEGATQVFRPINDNRIILGAVGE
                     LQFDVVAHRLQYEYNVDCVYDTINVSTARWVRCDNPKKLEEFERKVIGNLALDGGNNL
                     TYLAPTMVNLNLTIERWPDITFVATREH"
     gene            complement(3170..4198)
                     /locus_tag="PK389_01725"
     CDS             complement(3170..4198)
                     /locus_tag="PK389_01725"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQY22464.1"
                     /translation="MSNSFLVIVGLGLFLGSAALVRLFCYYAVKQGLIAIPNARSSHQ
                     VATPLGAGVVFVLLWFLCLLVGNVYHLFSVHVLLLFVPSTLVVSLVGFWDDHRELSAR
                     KRLLVQVLVAIFCVIMLGDVTALHLSRYTTLTLGWAGLVLTVLSIAWSINLYNFMDGI
                     DGLAGVEALFVFGIGGFFFWHVGAIPLALLTWTLVATVAGFLVWNWPKASVFMGDVGS
                     YFLGFLVGLFSLIADHLYHIPITVWIILYGVFWFDTTMTLIRRFIRHENLAMAHREHA
                     FHRLHRAGYSNTQVLWGVIALNTLLACIAIWAFKTDSFKWGFGLTLVILTAVYLKIEQ
                     RLPMAKTQ"
     gene            complement(4284..>4618)
                     /gene="murD"
                     /locus_tag="PK389_01730"
     CDS             complement(4284..>4618)
                     /gene="murD"
                     /locus_tag="PK389_01730"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="UDP-N-acetylmuramoylalanine--D-glutamate ligase;
                     involved in peptidoglycan biosynthesis; cytoplasmic;
                     catalyzes the addition of glutamate to the nucleotide
                     precursor UDP-N-acetylmuramoyl-L-alanine during cell wall
                     formation; Derived by automated computational analysis
                     using gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HQY22465.1"
                     /translation="GLADTISGKWVLIAGGVGKNADFSPLMSVVQKHCKAVILIGEAT
                     ETLYSLLHGVVNCVRASNMTDAVHVAANCVKAGDGVLLSPVCASYDMFKNFEERGNAF
                     KAAVKKLP"
BASE COUNT         1429 a         1092 c          876 g         1221 t
ORIGIN      
        1 aagtgattaa agcaacacat aaaaaaatag ccaaggtcga cagcaagata aaactaccct
       61 cttgtaaccg acgccatagc accaggcgcg tagcagctgt caatccaggt ttagatctgg
      121 ctttagattc tggttttgaa agggtttttc gctttttctt catgctaacg ctgtcatcca
      181 catttatata cgacttatca tctatttaat cataaacact ataccgcttt tttgcagctt
      241 agcgtatatt accttgattt gaagctgaga tctttatgca aaataccaaa cactgtcgat
      301 tactcatttt aggctctggt cccgctggtt atactgcggc tgtgtacgcc tcgcgtgcca
      361 atttaaaccc cgtgctgatt acgggtatgg aaaaaggcgg acaattaacc accaccaccg
      421 atgttgataa ctggccaggg gatatcgaag gtctaacagg tcctgcttta atggatcgca
      481 tggaacaaca tgccaaacgt tttaatactg aaatcatttt tgatcacatc gccgaagtta
      541 agttaagtag caaacccttt gaacttatcg gggattccaa aaagagatac accgcagatg
      601 cgcttattat tgcaacaggt gccagagcca aatacctcgg cattccctca gagcaggctt
      661 ttatgggcaa aggcgtctcg gcttgtgcaa cctgtgatgg ctttttctat aaaaaccaag
      721 gtgttgctgt tattggcggc gggaataccg ctgttgaaga agctctctac ctatcgaata
      781 ttgcgggtca tgttaccctt attcaccgtc gtgataaatt taaagcggaa aaaatcttaa
      841 tcacacaatt aatgaaaaaa gttgagactg gaaatatcac tattttgtgg aacacagtgc
      901 tcgatgagat tttgggcgat gaaaccggtg tgacgggcat tcgtgtaaca gaagtcacaa
      961 gcaaaagcca acaagatatt gcacttcagg gcgtttttat tgccattggc cacgtgccga
     1021 atacctccat atttgaaagt cagctcaata tgttcaatag ctatatcacc gtacagggtg
     1081 gttctgcggg caatgccact gcaacgaata tcccaggcgt atttgccgcc ggagatgtcg
     1141 ccgatcatgt ctatcgacaa gcgattacct ctgctggctc tggctgcatg gcggctttgg
     1201 atgctgaaaa atatcttgat gcaatgcagg tagcatctta gactgacatc tgctaatatc
     1261 tcttcattat ttattgaggt ttgaagctta attatggcca aagaagaagc gattgaaatg
     1321 gaaggtacgg tcatcgaaaa cctacccaac acacatttta gagtacaact agaaaacgga
     1381 catatggtga ttgcacacat ttctggccgt atgcgtaaaa attatattcg aattttaatg
     1441 ggcgatcgcg tcacggttca actaacccct tatgatctca caaagggacg cattactttt
     1501 agaaaccgtg aggccaaaga aattaaagag gccgaagccg ctgccacaga acccaagaaa
     1561 gattaatgct cccgcgttgc aacaaacgta atatccggcc aacgttcgat ggttaaatta
     1621 agattcacca tcgtgggtgc taaataggta aggttattgc caccgtctaa agccagattg
     1681 ccgatcactt ttcgttcaaa ttcctctaat tttttcggat tatcacatcg aacccatcgc
     1741 gcggttgaaa cattaatcgt gtcgtaaaca cagtcaacat tgtattcgta ttgtaaccga
     1801 tgcgcgacga catcaaattg caattcgcca acagcgccca gaataatacg attatcgtta
     1861 atgggtctaa agacttgggt agcgccttct tctgataact ggatcaggcc tttttgtaag
     1921 gcttttgctt ttagtggatc tttaggtcga accgcacgga ataactccgg cgcaaaataa
     1981 ggcacgccaa taaatttaag attctcaccc tgcgtaaagg tatcgccaat ttgaatggtg
     2041 ccatgattat gtaacccaat gatatcgccc ggccatgctt ccatcacccg atcgcgctcc
     2101 cctgccataa aggttaaggc atttgaaatt tgaatctctt tagccaatcg cacatggcgc
     2161 attttcatgc cttgttcgta ttttccagag catatccgta aaaaagcaat acgatctcga
     2221 tgctggggat ccatattcgc ctgaatttta aaaacaaaac ctgtaaattt tgcttctgtt
     2281 gcctcaacac tgcgtgtttc tgtttctcgc gattgtggcg atggcgcata gtttgcaaaa
     2341 gcatctaaga gttcttgcac accaaaatta tttaaagccg aaccaaaaaa tacaggagtt
     2401 agctcccctt ttaaataagc atctttatca aatccatggc ttgcaccttg caccaaagat
     2461 aattcctctc gaaattcttt ggcactctct acaccaatta aacgatctaa ttctggatta
     2521 ttaagacctt gaatgacctg ccctgcttgt atgcgttcgt ttttaccagg ttggtaaaga
     2581 caaatactat caaattctaa gtgataaatg cctttaaaat gcttccccat accaataggc
     2641 caagtcacag gtgcacagcg aatatttaat atgcgttcta tttcatctaa aagatctaca
     2701 ggttctcgac tatcgcgatc taacttatta ataaaagtaa taatcggtgt atcccgcaag
     2761 cgacagactt cgagtaactt aatcgttcga ggttctaccc cctttacggc atcaattacc
     2821 attagtgcag aatcaacagc cgtcaaggtc cgataggtat cttcagaaaa atcttcatga
     2881 cctggcgtat ctaacaggtt gatgatgcga tttttatacg gaaactgcat cacagatgtc
     2941 gtgaccgaaa tccctcgctg cttttctagc gccatccaat cggatgttgc atgtttgctg
     3001 gctttacgcc ccttcactgt accggcaagc tgtatagcac ctccatacaa caacagtttt
     3061 tcagtgatcg ttgtttttcc cgcatcgggg tgagaaataa tggcgaaggt gcagcgggca
     3121 ggaaatcccc ccggcccccc tttgtcaaag gggggaataa tgtgtatgct cattgtgttt
     3181 tcgccattgg taatcgttgt tcaattttca aataaaccgc cgttaaaata accaaagtta
     3241 aaccaaaacc ccacttaaaa gagtccgttt taaaagccca tatcgcaatg caagctaaaa
     3301 gcgtatttaa tgcaataacg ccccatagca cttgcgtgtt cgaataacct gctctgtgca
     3361 atcgatgaaa agcatgctct cgatgcgcca tggccaagtt ttcatgtcgt ataaaccttc
     3421 taattaaagt catcgtggta tcaaaccaaa aaacaccata caaaataatc caaactgtga
     3481 tagggatatg atacaaatga tccgcaatca acgaaaacaa tcccaccaaa aaccctagaa
     3541 aatagctacc cacatcaccc ataaaaacac tggctttggg ccaattccac actaaaaaac
     3601 ccgcaactgt tgcaacaagt gtccatgtta acaacgcaag gggaatagca cctacatgcc
     3661 aaaaaaagaa tcccccaatc ccaaaaacaa ataaggcttc caccccggca agaccatcta
     3721 tcccatccat aaaattataa agattaatag accaagcaat acttaatacc gttaaaacca
     3781 aacccgccca acccaaggtg agtgttgtgt agcgcgataa atgaagcgca gtcacatctc
     3841 ccagcataat cacacaaaaa atggccacca atacttgcac cagtagccgc tttcgtgccg
     3901 atagctctct gtgatcatcc caaaatccaa ccagtgaaac aacaagcgtg cttggtacaa
     3961 acaaaagcaa aacgtgtacc gaaaataaat ggtatacatt gccaacaagc aggcaaagaa
     4021 accacaataa aacaaacaca acacccgctc ccagcggcgt tgctacctga tgcgacgatc
     4081 gggcattcgg aatagcaata agaccttgct ttacagcata gtaacaaaat aaacgcacca
     4141 aagcggctga tcctaaaaat agccctaggc caacgatcac caaaaatgag ttactcaact
     4201 tgttttatct tcggctttaa ataatccata agggatttta acatagattg aaagggtgga
     4261 tacagcagtt ctgccctata tatttagggt aattttttaa cagcggcttt aaaggcattg
     4321 ccccgctctt caaaattctt aaacatatcg tagctcgcac aaaccggcga taacaaaaca
     4381 ccgtcgccag cttttacgca attagcagcg acgtgaaccg catctgtcat attacttgca
     4441 cgtacgcaat taacaacgcc atgcaataaa ctatataatg tttccgttgc ttcaccaatt
     4501 aaaataacgg ctttgcaatg tttttgaaca acggacatca aaggcgaaaa atcagcattt
     4561 tttccaacgc cacctgcaat caatacccac tttccagaaa tcgtatccgc caatcctt
//
LOCUS       DAORVT010000055         4616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_13633_length_4616_cov_2.966674, whole genome shotgun sequence.
ACCESSION   DAORVT010000055 DAORVT010000000
VERSION     DAORVT010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13633_length_4616_cov_2.966674"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PK389_01735"
     CDS             <1..378
                     /locus_tag="PK389_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22466.1"
                     /translation="KFSHYKVSLQAGLWDKASLEYLLRSGESPWAFEILGSIRAHGYP
                     KAFLSLVNDEPIHYINASHLGHIVPEAIAYTKAQNIGFDPGNFPRLGKFNWQISKRTW
                     LERWKKLSGFLKHPELFYEFDAS"
     gene            complement(401..2038)
                     /locus_tag="PK389_01740"
     CDS             complement(401..2038)
                     /locus_tag="PK389_01740"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY22467.1"
                     /translation="MGTSSQAHATAEPSVKQNNLILYNILVAEIAASRGMRSIALHYY
                     LKAIQQTNDPGVAEASTLLAISLEAPNEALISAECWAKEDPLNLQAQLITMTLLISQS
                     VDKALPYLTRVIELDPKQIDHSIMEVQSRLSESSAKNLKEALNKIALARPNDPYAHLI
                     AAESAVEQRDMNSATQWVDSALNQKPDLTSAIELKARLIRYQKNSDTPAIRFIKKQLE
                     IFPKNNELRFFYANVLLDTAQTEAAKTELHQLTEDSKYGGSALLILADIYLKEKQWKE
                     ASNALKKAKKFPDAMDGAEYLLGQIDELQDNKSAAVERYSNVTKGPYQIPAVLRAIAL
                     LKETQSYKEAIYLIHNSNPSTFEEQKQLLLMEIDLLNESKTPDEAMQLANDILIKLPL
                     DEDVLYSRAVTAIKLKKWEMAETDLKQILKQNPNNANALNALGHALSFNNHRHGEALE
                     FINQALSLSPNNPLFMDTLGWAYYQSGNYHQSVHYLKIALDLSENPKIAAHLGEALWI
                     NNQKDEAKTILKKALETNKDDEELLNTLKRLQIELKN"
     gene            2199..3464
                     /gene="hemA"
                     /locus_tag="PK389_01745"
     CDS             2199..3464
                     /gene="hemA"
                     /locus_tag="PK389_01745"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HQY22468.1"
                     /translation="MALMVVGVNFQTATIDIRERVALDLQTGVEIAHWLIAEGVAREV
                     LIISTCHRTEIYCEGLTHADLFGMIIGRLGLSIETIEPFSYKHLDLAAVMHLMRVATG
                     LDSMIVGESEILGQLKKAYTAFANASTIGKYLGRLFQTTFAIAKDVRTQTGIGVNPVS
                     VAYVAARLAQHIFPDLAKASVLLVGAGDLIRLIAQHLIAMGVQKIGVANRTEANAARL
                     ALLFNAEYFCLEKMKEHLFEADIVITGCASALPIISEKKVASALSQRESKPMFIIDLG
                     VPRNVECEVGALDNVHLYCIDDLQKMVEKNKRFRHDAAESAEIMIQIAAVRFMDWLQA
                     QDSFKTLCIFRQKFEQIRDRLCQDALQRLHLGEAPDRVVKRLASDLTSRFLHEPTRRL
                     REAGLDREEALLTLTRDLFELNCETFYTK"
     gene            3442..4533
                     /gene="prfA"
                     /locus_tag="PK389_01750"
     CDS             3442..4533
                     /gene="prfA"
                     /locus_tag="PK389_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011760675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HQY22469.1"
                     /translation="MKLSIQNKLEFLIERHQELSNLLSSASIIQDQARFRALSKEYSE
                     LEPLVLGFRAYQVAKQNVESTTEMLKDPDLDIRSMAQEELKEALLLLKIQEETLYRLL
                     LPKDPNDHRNVFLEIRAGTGGDEAALFAGDLFRMYTRFVENQGWGTEILNQSFGEHGG
                     YKEVIVRISGKNVYAKLKFESGAHRVQRVPATESQGRVHTSACTVAILPEIEAIESFD
                     IDPSDLRIDTYRASGAGGQHVNRTDSAVRITHLPTGTVVECQDERSQHKNKARAMSVL
                     QSKLLVAEQTKQRLAQAETRRNLVGSGDRSERIRTYNFPQGRLTDHRINLTLYQLPSI
                     MEGNLSPVIDPLIREYQADLLSMMSDEGL"
BASE COUNT         1274 a          820 c         1052 g         1470 t
ORIGIN      
        1 aaattctcac actataaagt ttcgttacaa gcaggattgt gggacaaggc ctctttagaa
       61 tatttattac gttcgggaga aagtccttgg gcttttgaaa ttttgggttc gattcgcgcc
      121 catggctatc ctaaagcatt tttaagcctt gtgaacgatg agcctataca ttatatcaat
      181 gcttcacatt taggacatat tgtaccagaa gcgattgcgt atactaaagc acaaaatatc
      241 ggttttgatc cgggcaactt tccacgttta ggtaaattta actggcagat cagcaaacga
      301 acatggctag aaagatggaa aaagttatca ggttttttga aacatccaga attgttttat
      361 gagtttgatg cttcgtgaat acgcatgcag atttagaaat ctagtttttt aattcgatct
      421 gtaatctttt taaagtattt aacaattctt catcatcttt atttgtctct aacgcttttt
      481 tcaaaatagt ttttgcctca tctttttgat tattgatcca gagtgcttca ccgagatgtg
      541 ctgcaatttt agggttttcg cttaaatcga gtgctatttt caaatagtgg acggactgat
      601 gataattgcc cgattgataa taagcccaac ccaacgtatc cataaatagc ggattattgg
      661 gggaaagact taaggcttga ttgataaatt ccagagcttc gccatgacga tgattgttaa
      721 aagataaggc atgtcctaat gcatttaagg catttgcatt attcggattt tgttttagga
      781 tttgttttaa atccgtttct gccatttccc attttttgag tttgattgcg gtaacagcgc
      841 ggctatataa gacatcttcg tcaagtggta gtttaatcag aatatcgttg gctaattgca
      901 tggcctcatc cggtgtttta ctttcgttaa gcagatctat ttccatgagc agaagctgtt
      961 tttgctcttc aaaagttgaa gggttactgt tgtgaataag ataaatcgct tctttataag
     1021 attgcgtttc ttttaataaa gcgattgcac gcagtaccgc tggaatttga tagggtcctt
     1081 ttgtgacatt cgaataccgc tctactgcgg cgcttttgtt atcctgcagt tcgtctattt
     1141 ggcctaatag gtattcagca ccatccattg catctggaaa tttttttgct ttttttaaag
     1201 cattagacgc ttctttccat tgtttttctt ttaaatagat atctgccaaa atcagaagtg
     1261 cggatccgcc atatttagaa tcttcagtta attgatgtaa ttctgtttta gcagcttctg
     1321 tttgtgctgt atcaagtaag acattggcat aaaaaaatcg gagttcatta ttttttggga
     1381 atatttcaag ttgttttttg ataaagcgta tggcgggtgt atcagaattt ttttgataac
     1441 gaatcaagcg tgctttaagc tctattgcac tcgttaaatc aggcttttgg tttaaagccg
     1501 agtcaaccca ctgcgttgcg ctgttcatat ctcgttgttc tacagcactt tctgcggcaa
     1561 tgagatgcgc ataaggatcg ttcggtcgcg ctaatgcgat cttatttaaa gcttccttga
     1621 gattttttgc gctactttca gacagtcgac tttgaacttc catgatggaa tgatcaattt
     1681 gtttgggatc aagttcaatg actcttgtga gatagggtaa ggctttatca actgactggc
     1741 taataagaag cgtcattgta attaattggg cctgtagatt aagcggatct tccttggccc
     1801 aacattctgc actgattaac gcctcgttag gcgcctcaag tgaaatcgct aacagggtag
     1861 atgcttcggc aacgccggga tcatttgttt gctgtattgc ttttaaataa taatgtagcg
     1921 cgatggatct catcccgcga ctggcagcaa tctctgccac aagaatatta tacagaatca
     1981 ggttattttg ttttactgaa ggttctgcag tcgcgtgcgc ttggctacta gtccccatcg
     2041 tcaaacagga gattaccaga tatactgcca caatccatag tagatgggaa agacgcggat
     2101 ttttaaaatt ttttgaaaga cgatacatta gtttatccaa atttgttctc tagataacac
     2161 atcggcttag gggtagaaat ctaaaggatt tgatgtaaat ggctttaatg gtagtaggtg
     2221 ttaattttca aacagcgacc atcgatatac gagagcgcgt tgcgcttgat ctgcaaacgg
     2281 gtgttgagat agcgcattgg cttatagccg aaggcgttgc gagagaggtt ctgattattt
     2341 caacgtgtca tagaactgag atatactgcg aagggctgac acacgcagat ctatttggga
     2401 tgataatagg tcggctgggc ttatctatcg aaacaataga gccgtttagc tacaaacatt
     2461 tagatcttgc cgcggtgatg catttaatgc gcgttgcgac cggtttagat tcgatgattg
     2521 tgggagagtc ggagatttta gggcaattaa aaaaggctta tactgctttt gcaaatgcca
     2581 gtacgattgg aaagtattta ggacgtttat ttcaaacgac gtttgccatt gccaaggatg
     2641 ttcgaactca aacgggcata ggggtgaatc ccgtctcggt tgcttatgtt gcggctcgcc
     2701 ttgctcagca tatttttccg gatttggcga aagcctctgt gttgttagtc ggtgcaggcg
     2761 atttaattcg attgattgcg caacatctga tagcaatggg agtgcaaaaa ataggggtag
     2821 caaaccggac agaagccaac gcagcgcgtt tggctttgtt atttaatgct gagtattttt
     2881 gtttagaaaa gatgaaggaa cacttgtttg aagcagatat tgtgattaca ggatgtgctt
     2941 ctgccttacc cattataagc gaaaaaaagg tggcatctgc gctgagtcaa cgcgagtcta
     3001 aacccatgtt tatcatagat ttaggtgttc cgcgaaatgt agaatgtgaa gtgggcgcat
     3061 tagacaatgt gcatttatat tgtatagatg atttgcaaaa aatggtagaa aaaaataagc
     3121 gttttaggca tgatgctgca gaatcagcgg aaatcatgat acaaattgcg gcggttcgat
     3181 ttatggattg gctacaagcg caagattctt ttaaaacgct ttgtatcttt cgacagaaat
     3241 ttgaacagat tcgcgatcgc ttatgtcaag atgctttaca gcgtcttcat ttgggagaag
     3301 cgcctgatcg tgttgttaaa cgtttagcca gtgatttaac aagtcgattt ttgcatgagc
     3361 cgacgcgtcg tttgcgagaa gccgggttgg atcgcgaaga agcattatta acattaacac
     3421 gagatttatt tgagttaaac tgtgaaactt tctatacaaa ataagctaga atttttaatc
     3481 gaacgtcatc aggaactgag taatctcttg agctcagcct cgatcattca agatcaagcg
     3541 cgttttcgtg cattgtctaa ggaatattca gaattagagc ctctggtttt gggttttcgt
     3601 gcgtatcaag tcgctaagca aaatgtggaa tcaacgacag aaatgttaaa agatcccgat
     3661 ctcgatatac gatcaatggc acaagaagaa ttaaaagaag ctttactgtt actcaaaatt
     3721 caagaagaaa cgttatatcg ccttctgtta ccaaaagatc cgaatgatca tcgaaatgtc
     3781 tttctcgaaa ttcgtgcggg gacaggggga gatgaagcgg cgttgtttgc aggtgattta
     3841 tttcggatgt atacccgttt tgtagaaaat cagggttggg gcaccgaaat tttgaatcag
     3901 agttttgggg aacatggggg ttataaagaa gtcattgttc gcatttctgg caaaaatgtc
     3961 tatgctaaat taaaatttga atcgggtgcg catcgtgttc agcgtgtgcc tgccacagaa
     4021 tctcagggcc gtgttcatac ctcagcttgt accgttgcca tattgccgga aattgaggcg
     4081 atagaatctt ttgatatcga tcccagtgat ttgcgtattg atacctatcg cgcctcgggt
     4141 gcgggtggac agcatgtgaa tagaacagat tctgctgtac gcattaccca tttaccgacc
     4201 ggaacggtgg tcgagtgcca agacgaacgt tctcaacata aaaataaagc gcgtgcgatg
     4261 agtgttctgc aatctaagtt attggttgcc gaacaaacga agcaacgttt agcgcaagca
     4321 gaaacaagac gaaatttggt gggtagtggg gatcgttcag agcggatccg tacttataat
     4381 tttccgcaag ggcgattaac cgatcatcgt attaatttaa cgctgtatca attgcccagt
     4441 attatggaag gcaatctatc tccggtgatc gatcctttaa ttcgcgagta tcaagcggat
     4501 ttattgtcga tgatgagtga tgaaggcttg tagtgattag tattcacagt attttgtcta
     4561 ttgctttaaa gtcgagcaat ttgacatccc tagaagctga agtattatta gcccat
//
LOCUS       DAORVT010000056         4585 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_13782_length_4585_cov_3.045695, whole genome shotgun sequence.
ACCESSION   DAORVT010000056 DAORVT010000000
VERSION     DAORVT010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13782_length_4585_cov_3.045695"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="PK389_01755"
     CDS             complement(<1..1038)
                     /locus_tag="PK389_01755"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQY22470.1"
                     /translation="MKNYDVVIIGAGAAGLMCAIEAGKRQRSVLVLDHANKPGKKILM
                     SGGGRCNFTNLNIISENYISHNPHFCKSALSRYTAQDFIYMVKKHQIKFYEKTLGQLF
                     CENKSQEILNMLLQECRDTHVDIRLNTTINKIEQQAEQGFNIRCHSDVQEEIQCQSLV
                     VATGGLSVPTLGASPFGYKIAEQFNIKVWPTRAGLVPFTLHPLDKEKYALLSGVSVKC
                     TARVAQKSFSEKLLFTHRGLSGPAILQISSYWQPGETVAINLLPEIDLLFSLKKAQQE
                     HPKKQLHTIMGEYLPKRVVESFLESKLANKTLKTLSLRELETVVKHFCAWSLKPNATE
                     GYRTAEVTLGGV"
     gene            1423..1716
                     /locus_tag="PK389_01760"
     CDS             1423..1716
                     /locus_tag="PK389_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005345154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HQY22471.1"
                     /translation="MYAFIEKLKSLPFVDEIWLFGSRARGDHRERSDIDLAIICPHAT
                     EQDWFQIMDIIENADTLLKIDCIRFEKSRISQELYGNILKERKVIYAKNTDQV"
     gene            1691..2083
                     /locus_tag="PK389_01765"
     CDS             1691..2083
                     /locus_tag="PK389_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014409893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HI0074 family nucleotidyltransferase
                     substrate-binding subunit"
                     /protein_id="HQY22472.1"
                     /translation="MLKTQIKFEKFCKALATLEAIYLKPVMDDRANIDATIQRFEFTF
                     ELGWKFLKDYFAEKGVILNYPKEIIQEAFAVGLINNESLWLQMLFDRNMTSHTYNEQL
                     ADEIYHRIKNYVPEFKKIIVSGARNEEK"
     gene            2097..2987
                     /locus_tag="PK389_01770"
     CDS             2097..2987
                     /locus_tag="PK389_01770"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HQY22473.1"
                     /translation="MNFQYSTLFNAPLSLATYTLLACAILSLWIKRDYAVWATLFVVA
                     TICGIISNRLQWPALLFMMLFAGLCYFTFHARHLKLKITCGIFVYIMSILLWYHQIPG
                     FANWQIVNDTLLSADALPFSMYLNFDKPLVGLLILGFGTLPLLKSKKDGYSVFIKTVP
                     IAIIGTFVIAGLAYAFGYIKFEPKWDNFFIVWFTSNLFLTVIAEEVLFRGFVQKYLSL
                     AFAKIWFGSGLALLIASILFAVVHTGGLTYIFLAFVSGILYGWVYIKTNRIEASILTH
                     LFLNSVHILLLTYPGQASIT"
     gene            3107..>4585
                     /locus_tag="PK389_01775"
     CDS             3107..>4585
                     /locus_tag="PK389_01775"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HQY22474.1"
                     /translation="MNIIRSTSVIVFAMLSFFTSVEARWATPDDLDIITESYNRDLQI
                     NKDGTYVETSDLTLVATKESGKDRLVSFPLVYNAQSSKLVVLEAKTICNGIEYPVDLK
                     EIEDKPLASSPQGFDQNRQLLIAFPEMALNAKIFIKYQTTVTEVPVPGFFSTQYIYGA
                     DAYEKNSKVHVRSAIPFFVEVNDPEKVLTITEKQSNKQYEMDIILIKPIIKVAIDEQN
                     MSLDGKLLPWVTISTLDQWPKLGAMLIPQYEEKINQTLPPLFETIAIEAEKKTTTIEK
                     INTVTSKLSETITYMGDWRTIKGAYIPRPLAEIVKTKIGDCKDFSAATVAILRRLGIA
                     SNVALVYRGWEPSRSPNHLPTLNQFNHAFVMVEDKDKILWIDPTNFSSYAQGIYPDVA
                     NRDALVLKPLAPSLLHTQALDLKNSNITLSEKISMSKEAFDTAKVEGELSLSGTVVLP
                     LTGADLRFSKEIISRAIINTVANESRVVNWHVGDYNLASRIVD"
BASE COUNT         1377 a          755 c          936 g         1517 t
ORIGIN      
        1 aacaccccca agcgtgactt ctgcggtacg gtaaccttct gttgcattgg gctttagcga
       61 ccaggcgcaa aagtgtttta ccacagtttc cagttctctc aaagacaatg ttttcaatgt
      121 cttgttggct agttttgact ctaaaaacga ctccacgacg cgtttaggca aatattctcc
      181 catgatagta tgaagctgct ttttaggatg ttcttgttgt gcttttttca agctgaacaa
      241 cagatcgatt tccggtaaca ggttaatggc gacagtttcg ccaggctgcc aatatgatga
      301 gatttgtaat atggcgggac cgcttaaacc gcgatgggta aacaatagtt tttcagaaaa
      361 acttttttgt gcgacacgag cagtgcattt tacagaaacg ccagaaagca gcgcatattt
      421 ttctttgtct agtggatgta aggtgaaagg cactaagcca gcacgtgttg gccagacttt
      481 aatattaaat tgttctgcta tcttatagcc aaaggggctt gctcctaaag tgggtacaga
      541 caatccgccg gttgcaacga ctaaggattg acattgaatc tcttcctgaa catccgaatg
      601 gcaccgtatg ttaaaaccct gttcggcttg ctgttctatt ttatttatgg ttgtatttaa
      661 gcgaatatcg acatgggtat ctctacattc ctgtaataac atatttaaaa tttcttgtga
      721 tttattctca caaaaaagtt gtcccaatgt tttttcataa aatttaatct gatgtttttt
      781 aaccatgtag ataaaatctt gagccgtgta tcgactgagt gcggatttgc aaaaatgtgg
      841 attatgcgaa atataatttt ctgaaataat attgagattc gtgaagttgc atctgccacc
      901 gccggacatc agaatcttct tgccaggttt gttcgcatgg tcaagcacta aaacagatct
      961 ctgtcgcttt cccgcttcaa tggcacacat taaccccgcg gcgcctgcgc caataataac
     1021 gacatcatag tttttcattg aaaaatatta acacagtttt ctttctggat gctagtgtta
     1081 aggttttatc tgatcgcaag ttgagggcat agttgtggtg cagcgaatat tttttctaat
     1141 aattttggat tgacatttaa ctgcgaatat attttagttt tccagaagtg gctttgtggc
     1201 agttgctgca aggtggcgct ggtatgcatg atgcaatatt gtcaaaatta acccaatttt
     1261 ttgaaaggaa atgtattgtt aaacaaaaat aatagttgag gcatttttat tttttatata
     1321 tactgctaat attatgaagg atttgatata aagagagtat gtgttagatg ctgtgcgagt
     1381 ttatgatgtg aaaaatctgt tgttgaaaag gatctgagac agatgtatgc atttattgaa
     1441 aagttaaaat ctttgccgtt tgttgatgaa atatggcttt ttggttcgcg tgccagaggg
     1501 gatcatcgag agcgttctga tattgattta gctattattt gtccacatgc aacagaacaa
     1561 gattggtttc agatcatgga tatcatagaa aatgcagata cgctattgaa gatagattgt
     1621 attcgttttg aaaaatcacg aattagtcaa gaactttatg gcaatatatt gaaagagaga
     1681 aaagtgatat atgctaaaaa cacagatcaa gtttgagaaa ttttgtaagg cacttgcaac
     1741 actagaagca atttatttga aacctgttat ggacgatagg gctaatattg atgcaacaat
     1801 acagcggttc gaatttactt ttgagcttgg atggaaattt ctaaaggatt attttgcgga
     1861 aaaaggtgtc attttaaatt atcctaaaga aattattcaa gaagcttttg cagttggcct
     1921 gataaataat gagagtttgt ggcttcagat gctattcgat cgtaacatga cttcacatac
     1981 gtataatgag caacttgcag atgaaatata tcataggatc aaaaattatg tccctgaatt
     2041 taaaaaaatt attgtatcag gcgcccgtaa tgaggaaaaa taacgagatt cactgaatga
     2101 actttcaata ttcaacatta tttaatgcac cgctgtcctt agcgacttac accttgttag
     2161 cctgtgccat tttatctttg tggataaagc gtgactatgc tgtttgggcc acgctttttg
     2221 tagttgccac aatatgcgga ataatcagta atcgactaca gtggcccgca ttattgttta
     2281 tgatgttatt tgcggggctc tgttatttta cttttcatgc cagacatctt aaattaaaaa
     2341 tcacttgtgg catatttgta tatattatga gtattttgct ttggtatcat cagattcccg
     2401 gttttgctaa ttggcaaatc gtgaatgaca cattgttgag tgcggacgct ctgccgtttt
     2461 caatgtattt aaattttgac aaaccccttg ttggtctttt gatattaggt tttggtactt
     2521 tgcctttatt aaagtccaaa aaagacgggt atagcgtttt cattaaaaca gtgcctatag
     2581 caatcatagg tacttttgtt attgcaggat tagcctatgc ctttgggtat ataaaatttg
     2641 aacccaaatg ggataatttc tttattgtgt ggtttacaag taatttattt ttgacagtga
     2701 tcgcagaaga agttttgttt agaggctttg ttcaaaaata tttgtctctt gcctttgcga
     2761 aaatttggtt tggcagtggg cttgcactat tgattgcctc gatattattt gcagttgtgc
     2821 acacgggtgg tttgacttat atattcctgg cttttgtttc gggaatttta tacggctggg
     2881 tttatataaa aactaatcgc atcgaagcga gtattttgac acatctattt ttaaattctg
     2941 ttcatatttt attattgact tacccaggtc aggcaagcat tacatgaaaa aataaaaaag
     3001 cagggtagat tttttagtat tcaaattgac cagattgctt tcttcttgtt aaaatcttaa
     3061 agcttttgag cctattgtct ttaactaaga aatggaagtt atgttaatga atattattcg
     3121 tagcacctcg gttattgttt ttgccatgtt gagttttttt acttctgtcg aagcgcgttg
     3181 ggcaaccccg gacgatttag atatcatcac ggaatcttat aatcgggatt tacagatcaa
     3241 taaagatgga acatatgtag aaacaagcga tttaacatta gtggcgacca aagaaagtgg
     3301 aaaagataga ttggtttctt ttcccttagt ttataatgcg cagagctcta aattagttgt
     3361 gttagaggct aaaaccattt gcaatggtat tgaatatcct gttgatttaa aagagataga
     3421 agacaaacct ctggccagta gtccacaggg ttttgatcaa aatcgacaat tgttaattgc
     3481 ttttccagaa atggccttaa atgcaaaaat ctttataaaa tatcaaacta cagtaacaga
     3541 agtgcctgtg ccaggctttt tttcaacgca atatatttat ggtgctgatg cctacgagaa
     3601 aaatagcaag gtacatgtta ggtctgcgat cccatttttt gtagaggtga atgatcctga
     3661 aaaagtcctc actattacag aaaagcagtc taataaacaa tatgaaatgg atattatctt
     3721 aataaaaccg attattaaag tagccataga tgaacagaat atgtcgcttg atggaaaatt
     3781 actcccctgg gtgactattt ctacattgga tcaatggcca aaattagggg caatgttgat
     3841 tccacaatac gaagaaaaaa taaatcaaac attaccgccg cttttcgaaa caattgccat
     3901 tgaggccgaa aaaaaaacaa cgactatcga aaaaattaat accgtgacct caaaattatc
     3961 agaaacgatt acctatatgg gagattggcg tacaattaag ggtgcctata tcccgaggcc
     4021 gttagcggag attgttaaaa caaaaattgg cgattgcaaa gatttttctg cagcaacagt
     4081 ggcaatctta agacgcttag gcattgcatc caatgtggct ttggtttatc gcggttggga
     4141 gccaagtcga tctccaaatc atcttccaac tttaaatcag tttaaccatg cttttgtgat
     4201 ggttgaagat aaggataaga tactttggat agatccgacc aattttagta gttatgcgca
     4261 ggggatttat cctgatgttg ccaatcgcga tgccttagtg ctgaaacctt tagcgcctag
     4321 tcttttgcat acacaagcac tcgatctcaa aaattcaaac ataacgcttt cggaaaaaat
     4381 ttctatgtca aaggaagcgt ttgatactgc taaagtagag ggtgaattgt cgctttcggg
     4441 caccgttgtc ttgccattga caggtgctga tctgaggttt tctaaggaaa ttattagtcg
     4501 agcgattatt aatacggtag ctaacgaaag tcgtgttgtt aattggcatg taggtgatta
     4561 taatttagca tcgcgcatcg tcgat
//
LOCUS       DAORVT010000057         4549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_13996_length_4549_cov_3.586115, whole genome shotgun sequence.
ACCESSION   DAORVT010000057 DAORVT010000000
VERSION     DAORVT010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13996_length_4549_cov_3.586115"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="PK389_01780"
     CDS             <1..869
                     /locus_tag="PK389_01780"
                     /inference="COORDINATES: protein motif:HMM:NF015688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HQY22475.1"
                     /translation="KLPFLNDKQRLQALEDTRRKQQEEQKKRAKQTQLSEVQQKQLAA
                     LMTSMEAQSKKAFDDWAAITPQAFVQGDKKDTKDDTGSTARSTGGDGKGGNATGTVLI
                     KAGTILFAVLDTAVNTDEPGPVMATIVQGEFQGAKIIGTTQMSSQPGSDRPEKVTLNF
                     ATMSTPSLPKSLPIQGVAIDTDTARTALASDVDHHYLLRYGTLFASSFMNGYAKVITS
                     GGTVQTNSGTGATSTTTPKLSGRKEVFAALGEVGKKFGDATSTYFSTPNTITVNAGTG
                     IGLLILADVTAQ"
     gene            893..1861
                     /locus_tag="PK389_01785"
     CDS             893..1861
                     /locus_tag="PK389_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22476.1"
                     /translation="MNDERKSLDTEQVDESFNEDFDFGEGETQSRPMETTNTNGHNKK
                     IIGFIFAIVLIVVLVMGYRVYSPKLLGSKNPLNTATLTKKPAPSTTINPIHAPKNVTE
                     TPEETKKQSDEFAAAFSGITSETVPAEPKPATVSVPEHLPPLLTPTTSHSPEPEADVA
                     KLHEALNKLNTQIDSILSQVKYLDAYSREVSDNLNKLNESISTMDDKLLALTNTTSTL
                     SKDVGTVKSEVGQVREVLREDGLDINPASLSKGRQGRLEKEGQVAIAEPEYTVHAVVP
                     GRAWLKSSKGQIVTVAEGDSIGNYGKILVIDAANGVVLTSSGVAFR"
     gene            1848..2489
                     /locus_tag="PK389_01790"
     CDS             1848..2489
                     /locus_tag="PK389_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22477.1"
                     /translation="MLSVKLAYLGSLLYFVSENVYAAPDIQTILNNVSRIIVPLTAMV
                     LIVSFVAGVYIIIHALTMMKKFGNMSMQAQPGELGGPLVQLVVGAVLIYLPSSTDTLA
                     NSLFGTSNSIFGSNSSSVNYSNAGYGSELLGYVASNSFAEQWASMANTLVLYIQFVGF
                     LSFVKGWFILSKSGGHGTQQGTVAKGITHILGGIVAINFVAASNIIYNTIFGT"
     gene            2530..2898
                     /locus_tag="PK389_01795"
     CDS             2530..2898
                     /locus_tag="PK389_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein IcmD"
                     /protein_id="HQY22478.1"
                     /translation="MKLKRSIVITTVVALVALAPSILLAADITDIAKTVTTQATAIAK
                     LLSVTAYVAGVGFALAGVLQFKTHKENPQQTPLSKPVVMIVVAACLLFLPTILTIAGA
                     SLFGASPISSAPSGAGLGGT"
     gene            2898..3512
                     /gene="icmJ"
                     /locus_tag="PK389_01800"
     CDS             2898..3512
                     /gene="icmJ"
                     /locus_tag="PK389_01800"
                     /inference="COORDINATES: protein motif:HMM:NF038221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmJDotN"
                     /protein_id="HQY22479.1"
                     /translation="MDLLPLTLRAERGNWQRFMNRKANKAFAVVRDKVFARDDFSCRY
                     CGFQIKQFQEVLNIDQNYDNNQLDNLVTACHFCAQCFFLDSVGLDGKSGGTIIYLPEI
                     SQADLNHFSRTLFCSMLRDPPYKGKLQAAYLSLMDRAKPVEEVFGPHTQNPIIFGQTI
                     IDTGLTAAQLKHPIFSELKLLPTRKYYKTETEYWKSTVFANIPL"
     gene            3531..>4549
                     /locus_tag="PK389_01805"
     CDS             3531..>4549
                     /locus_tag="PK389_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein IcmB"
                     /protein_id="HQY22480.1"
                     /translation="MSIIDPVLDSIDGVLAWLSTSLGQLAESYCDLETADGEETLVGR
                     DGSLVSILKIDGATFLIGPDEFTRMHDTLTQSLQTCLSRPGHAIQVFFQHDKDTITHE
                     LNHILAPARQTAKQLGLSLDDLFSERVQELRRYCASESMYLVVWSLPSSLTKAQQEQS
                     IKDRTERAKTVKIPLIRNAQNFMKAIPELRDGHDSLVRSLVNDFNLMGLLTKLLDVHA
                     ACHAIRQSVDPDFTDPKWQPYLPGDAIPVRETKNFAGDISDVLWPSLAKQLIPRDGEI
                     LNLRTARLGDRIYATVSIDLFPKELAAFGVLFNRVLNAQIPWRISFLISGGGIQALGL
                     KSTLAT"
BASE COUNT         1354 a         1008 c          962 g         1225 t
ORIGIN      
        1 aaaaattacc cttcttaaac gataaacaac gtcttcaagc ccttgaagac acgcggcgca
       61 aacaacaaga agagcaaaaa aaacgggcta aacaaacgca attaagcgaa gtacaacaaa
      121 aacaacttgc tgcgttaatg acaagtatgg aagcacaatc caaaaaggct ttcgatgatt
      181 gggccgccat taccccacaa gctttcgtgc aaggcgataa aaaagacact aaagacgata
      241 ccggtagcac tgcacgcagc acgggcggtg acggcaaagg cggaaatgca accggtacgg
      301 tactcattaa ggcgggaacc attttatttg cagttttaga cacggctgtc aacacggatg
      361 agccaggtcc tgtgatggca actatcgtac aaggtgagtt tcaaggtgct aaaatcattg
      421 gtacaacaca aatgtcctca caacccggca gcgatcgtcc agaaaaagtg acccttaatt
      481 ttgcgacaat gagcacacca agtttaccca agagtctgcc cattcaaggt gtcgccattg
      541 atacggatac cgcacgaaca gcgctggctt ccgatgtaga tcaccactat ctcttacgat
      601 acggcacatt gtttgcttca tcatttatga acggatatgc caaagtcatt acttccggag
      661 gaaccgtaca aaccaatagc ggaacaggcg caacaagcac cacaacaccc aaattaagcg
      721 gtaggaaaga agtgtttgca gccttaggtg aagtgggcaa aaaatttgga gatgcaacct
      781 ctacttactt ttcaacgccc aataccatta cggttaatgc aggaacagga ataggtttat
      841 taattttggc ggatgttaca gcgcaataat atgtaaatag ggactgacag tcatgaatga
      901 tgaacgcaaa tcgctcgata ccgagcaagt ggatgagagt tttaacgaag acttcgactt
      961 tggggaaggt gaaacccaaa gtcgtcccat ggaaaccaca aacacaaatg ggcacaataa
     1021 aaaaatcatt ggctttattt ttgccatcgt tctgattgtt gtgcttgtca tggggtatag
     1081 agtctacagc cccaagcttt tgggttctaa aaatcctctc aatacggcga cgttaacaaa
     1141 aaagcccgcc cctagcacaa caattaaccc aatacatgcg cctaaaaatg taaccgaaac
     1201 acctgaggaa acaaaaaaac aatctgatga atttgcagca gctttttcag gtattacctc
     1261 agagactgtt cctgctgaac caaaacccgc aactgtttct gtacctgagc atctacctcc
     1321 cctccttacc ccaacaacat cacactcgcc cgaaccggaa gcagatgtgg caaaacttca
     1381 cgaggcgctt aataagttga atactcaaat tgactctatt ttgagccaag tcaaatattt
     1441 agatgcttat agtcgagaag tgtccgataa tctaaataag ctcaatgaat ccatcagcac
     1501 aatggatgat aaattactgg ccctaacaaa taccacttct acattgtcta aagatgtagg
     1561 aacggtaaaa agtgaagtgg gacaagtgcg agaagttctc agggaagatg gactcgatat
     1621 aaacccagct tctttatcaa aaggaagaca aggacgtcta gaaaaggaag gtcaggtagc
     1681 cattgcagaa cccgaatata cggttcatgc cgtggtgccg ggtcgtgcgt ggcttaagtc
     1741 ttctaaaggt caaatagtaa ccgttgccga aggagattca attggtaact atggcaaaat
     1801 tctcgttatt gacgcagcga atggggtggt tctcacaagc tcgggcgttg ctttccgtta
     1861 agctcgccta cctcggatct ctgctttatt tcgtttctga aaacgtatac gctgctccag
     1921 acatacaaac cattctaaac aatgtcagca gaattattgt tcccttaacg gcaatggtgc
     1981 tgatcgtttc gtttgtggcg ggtgtctata taattatcca cgccctcacg atgatgaaaa
     2041 aatttggtaa tatgtcaatg caagctcaac cgggtgaatt gggtggccct ttggtgcaac
     2101 tcgttgtagg ggctgtctta atctatttac cctcctctac cgacacatta gcaaattctc
     2161 tttttggaac cagcaattct atttttggct ctaattctag cagtgttaat tatagcaatg
     2221 caggctatgg ttcggaactg ttgggctatg tggccagcaa cagttttgca gaacaatggg
     2281 cttctatggc aaataccttg gtgttataca ttcaatttgt agggtttttg tcgtttgtca
     2341 aaggatggtt tattctatct aaatcgggag gacatggcac gcaacaaggg actgttgcca
     2401 aaggcataac gcatatcctc ggtgggattg ttgcaataaa ctttgttgct gcgtcgaata
     2461 ttatctataa cacgattttt ggtacataat aagtgtatgg agatgttttg aataaaatag
     2521 gaggtcatga tgaaattaaa gcgtagtata gtcatcacaa cggtagtcgc gctcgttgca
     2581 cttgccccgt ccattttgct agcggcagat attaccgata ttgcaaaaac ggttacaaca
     2641 caagcaacag ccattgctaa actgctcagc gtaaccgctt acgtggcagg tgttggtttt
     2701 gcactggcag gcgttttgca atttaaaacc cataaggaaa acccacaaca aaccccatta
     2761 agcaaaccag tagtcatgat cgttgttgcc gcatgtttgt tgttcttacc taccattctg
     2821 acgatagcag gtgcttcgct gtttggagca tcacccatct cgtcagcacc cagtggggct
     2881 ggactaggcg gaacttaatg gacttactac ctctcacact tcgtgcagaa cgcggtaatt
     2941 ggcaacgctt tatgaaccga aaggcgaata aagcgtttgc cgttgtaaga gataaagttt
     3001 ttgcccgaga tgactttagc tgccgctact gcggttttca gatcaaacaa tttcaagaag
     3061 tcttgaatat tgatcagaat tacgataata atcagctgga taatttagta accgcttgcc
     3121 acttttgtgc tcagtgcttt tttttggatt ctgtcgggct cgatggcaaa agtggtggta
     3181 cgattatcta tttgcccgaa atcagtcagg ctgatcttaa tcatttttcc cgtactcttt
     3241 tttgcagcat gttacgtgat cctccttaca agggaaaatt acaggctgct tacttaagct
     3301 taatggatcg tgctaaaccc gtagaagagg tctttggacc ccatacccaa aatcctatta
     3361 tctttggtca aacgattatt gacaccggtt taacagcagc acaactgaaa cacccgattt
     3421 tttctgaact caagctgcta ccgacacgta agtattataa aaccgaaacg gaatactgga
     3481 aatccacggt ctttgcaaat attccactat agaaggtcta taatttaacg atgagtatta
     3541 ttgatcctgt attagatagc attgatggcg tactggcatg gctcagcaca tctctaggcc
     3601 aactggccga atcctattgt gatttagaaa cggcagatgg tgaagaaaca ctggttggcc
     3661 gagatggatc actggtttcg attttaaaga tcgatggtgc aaccttctta ataggacctg
     3721 atgaatttac acgaatgcac gacactttaa cacagtcctt acaaacatgt ttatctcgtc
     3781 cgggacacgc cattcaagtc ttttttcaac acgataaaga cactataacc catgaactta
     3841 atcatatact tgccccagca cgtcaaacgg ctaaacaact cggtctttca ttagacgatc
     3901 ttttctcaga acgcgttcag gaacttcgtc gatattgtgc ttcagaatcc atgtatctcg
     3961 tcgtgtggtc actcccctct agtttaacga aagcacaaca agaacaatcc attaaagatc
     4021 gtacagaacg cgctaaaacg gtaaaaatac cgcttattcg aaatgcgcaa aattttatga
     4081 aagccattcc tgaattaaga gatggtcatg actctcttgt gcgctctttg gtgaatgact
     4141 ttaatctaat gggtttgctc acaaaattgt tagatgtcca tgccgcttgc cacgccattc
     4201 gtcaatcagt tgaccctgat tttacagatc ccaaatggca accttatctg cctggtgatg
     4261 cgatccccgt ccgagaaact aaaaattttg cgggagatat atcggatgta ctctggccat
     4321 cactcgcgaa acaattaata ccgagggatg gcgaaatctt aaatcttcgc accgcacgtt
     4381 taggtgatag aatctacgct acggtttcta ttgatttatt tccaaaggaa cttgctgcct
     4441 tcggtgtttt atttaatcgt gttctcaatg cccaaattcc atggcgaatt tcctttttaa
     4501 tctcaggcgg tggcatacaa gctttgggat taaagtcaac gctggcaac
//
LOCUS       DAORVT010000058         4547 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_14007_length_4547_cov_2.876002, whole genome shotgun sequence.
ACCESSION   DAORVT010000058 DAORVT010000000
VERSION     DAORVT010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14007_length_4547_cov_2.876002"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..1136)
                     /gene="prmB"
                     /locus_tag="PK389_01810"
     CDS             complement(201..1136)
                     /gene="prmB"
                     /locus_tag="PK389_01810"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017377163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HQY22481.1"
                     /translation="MELTTLNVVLKWAEQSFKAAKLYYGHGTNNAWDEAVALALYVLK
                     LPPDVDACVGERVLTAHEKEIYLKLVNQRITTRTPVPYLTHEAWFAHQKFYVDERVII
                     PRSPMAELILNGFQPWLGKHMVHRVLDLCTGSACIAIACAKVFENAIVDAIDISEDAL
                     DVARKNCVLQNCESRVNLIHADLFSGCAGKRYDIIISNPPYVDSAEMRNLPPEYHWEP
                     RLALAAGSDGLAIVKQILREAPRYLTEKGLLFVEVGNTEEALKNQYPDFLGTWLEFEN
                     GGQGVFLLYAEDKSCWQGFNHLWVWFFNMDDVSYD"
     gene            1269..2486
                     /locus_tag="PK389_01815"
     CDS             1269..2486
                     /locus_tag="PK389_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22482.1"
                     /translation="MAHSDRHILENNRDFTYPLSAAHKFIHLYYHLKRPNAEQRTILA
                     TAYYTLSTQNNISCHRQKMHVNSAITLLKNITLQKRDNLWGSQIARAYFKRAELLEAK
                     EAFSLATNDYVDTIAVLEQFESEQILDEADRLLIAQSAISIADLIVNEQIDDKALNLN
                     HPLFYINKALEYLRAFPEPNDDILSTYAYAHQIAGISLSVFHFEESKEAFRVALLMAF
                     KTETIGISPLLTDIYTCLGLLYEQQYESCPIKKAPEYLLDHAMIYFGLALLFSPKETE
                     QDDEDVLTLDSFFDMIYRVLDPYISPLSHQLTCDLIDALIYAYYCVVNKSLPNKILQD
                     ELNQDDTLNNFAQHLYWLVSEAYRKQNPRAALNFLEILTHEAYENTLYAEDILSIITQ
                     KKSNNVYYLTSKK"
     gene            complement(2524..2733)
                     /locus_tag="PK389_01820"
     CDS             complement(2524..2733)
                     /locus_tag="PK389_01820"
                     /inference="COORDINATES: protein motif:HMM:NF024879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HQY22483.1"
                     /translation="MRNKMIVLTLVCVFLSGCAGMSRNESTGAAIGGVAGAVIGGSNG
                     GALGAGIGAAAGGLLGGAVGHTMDH"
     gene            2973..3974
                     /locus_tag="PK389_01825"
     CDS             2973..3974
                     /locus_tag="PK389_01825"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HQY22484.1"
                     /translation="MKILVTGGLGFIGSHIVEYHLNKNDAVYVVDNLSTGSLDNLSLF
                     QNNPDFQFDKADILTWQNMEKAVAWADRIYHMAAVVGNLRVIQEPINVIASNIAGTER
                     LFNTITKIGNTPRVILASTSEVYGKSSKSTMQEEDDLIIENPVHLHSNYAISKLSDEI
                     IGLSYDRINNIPTTIIRFFNTIGPRQTGTYGFVVPKFIAQACKGEPISIYGDGSQTRS
                     FGDVRDVVVALDLIAENIRTRYNILNVGVDHEITIKALANLVRERANSKSDIIYVPYK
                     KIYGMEFLDIMHRRPDLTKLHKLIQFKHKWTLEKTVDNLIGIFNENSLKPQLKNKNL"
     gene            complement(3971..>4547)
                     /locus_tag="PK389_01830"
     CDS             complement(3971..>4547)
                     /locus_tag="PK389_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22485.1"
                     /translation="IAFKKGQDMYAFSPRSVSLKTLQKYNRMTVSLQPYPQKYLDIGL
                     EYDKFSDKNTLKVLSLYPHATVLASQYLDIDIGPYATWQNFSLQDPVNGYYDQRSYQM
                     YQALSHFTIKQNENINYVLTFGAGAQKDETMLKPGFAGDIALRAIYGIYADWYLNISA
                     SATARNSSSLLYGSGTKYRIYAIEALLTKRF"
BASE COUNT         1449 a          970 c          813 g         1315 t
ORIGIN      
        1 ataaaaataa cattctcttt tttcagtcgc ccaactcgcc ctactcagac aacggtgctt
       61 tcaaaagaaa atattatttt tatccagacc ttaatataaa aattgctatg ccgactacca
      121 caatcagaat taaagccagg aatccaacag ttgtaaagct acgacttaaa tttgcttttg
      181 agaatgcttc aggattgcct ttaatcgtac gatacatcat ccatattaaa aaaccaaacc
      241 cacaaatggt taaaaccctg ccaacatgac ttgtcctccg catacaataa gaatacaccc
      301 tgaccaccat tttcaaattc taaccacgta ccgaggaaat ccggatattg gtttttaagc
      361 gcttcttctg tattacccac ttccacaaac aaaagtccct tttcggtgag ataacgaggt
      421 gcttctctca ggatctgttt tacaatcgct aaaccatctg atccagcggc taatgccaaa
      481 cgtggctccc agtgatactc gggtggtaaa ttccgcattt ccgcgctatc gacatagggt
      541 gggttagaga tgataatatc ataacgtttt cctgcacatc ccgaaaagag atcagcgtgg
      601 atcagattaa cacgtgattc acaattctgt aatacacaat ttttacgcgc gacatctaag
      661 gcatcttcag aaatatcaat cgcatcgaca atcgcgtttt cgaaaacttt tgcacaagcg
      721 attgcaatac aagcgctccc cgtacataaa tctagcacac ggtgcaccat atgtttaccc
      781 aaccacggct gaaatccatt taaaatgagt tccgccatgg gcgatcgcgg aataatcacc
      841 cgttcgtcga cataaaattt ttggtgtgca aaccaagcct catgcgtcaa ataaggcacc
      901 ggggttcttg tcgtaatacg ttgattaacc agttttagat agatttcttt ttcatgagca
      961 gtcagtaccc gctctccaac acaggcatcg acatcgggag gtagcttcaa aacatataaa
     1021 gccagagcca cggcttcatc ccaggcattg ttcgttccat gcccataata gagctttgcc
     1081 gccttaaaag actgctctgc ccactttaaa accacattta gggttgttaa ttccacaaag
     1141 acctcttaac atagagccag ggcggacaca atgatacgcc cccgcgattg ggtaaaaggc
     1201 cattttccgc tcaaagggtt tttggatcaa tgggttagaa gcattaagtt aaataactgg
     1261 ggtgtttcat ggcgcacagc gatagacata tattagaaaa caatcgggat tttacctatc
     1321 cgctttccgc tgcccacaaa ttcattcatt tgtactatca cttaaagcga cccaacgcgg
     1381 aacaacgtac cattctcgca actgcgtatt acacgctctc tacacaaaac aatatttctt
     1441 gccatcgaca aaaaatgcat gtgaattcgg ctatcacgct tttaaaaaat attactcttc
     1501 agaagagaga caatctgtgg ggctcgcaaa ttgcgcgcgc ttattttaaa cgcgcagagt
     1561 tattagaagc caaagaggcc ttttcactgg caaccaatga ctatgtcgat actattgctg
     1621 tgttagagca atttgaaagt gaacagatcc tcgatgaggc tgatcgtctc ttaattgcgc
     1681 aatccgcgat ctctattgca gatcttatcg tcaacgaaca aattgatgat aaagcgctta
     1741 atttaaacca ccccttattt tatattaata aagctttaga gtatttacgc gcattcccag
     1801 aaccaaacga tgatatttta tcgacgtatg cttatgcaca ccagattgcg ggtatttctt
     1861 taagcgtttt ccactttgaa gaatctaaag aagcgtttcg tgttgcgtta cttatggcgt
     1921 ttaaaacaga aactattggg ataagcccgc ttttgaccga tatttatacc tgtttagggt
     1981 tgttgtacga acaacaatac gaaagttgtc ctatcaaaaa agcgcctgaa tatttgctgg
     2041 atcacgccat gatttatttt ggcctggcgc ttttgtttag tcccaaagaa accgaacagg
     2101 atgatgaaga cgttttaaca ttagattctt tttttgatat gatataccgc gttttagatc
     2161 cttatatttc tcccctttcg catcagttaa catgtgacct aatcgatgct ctgatttatg
     2221 cttattactg cgttgtgaat aagagcttac ccaacaaaat attacaagat gaactaaacc
     2281 aagatgatac gctcaataac tttgcacaac atctctattg gttggtctct gaagcttatc
     2341 gtaaacaaaa tccaagagct gcattaaatt ttctcgaaat tctgacacat gaagcatatg
     2401 aaaacacgct atatgcagaa gatattttaa gtatcattac acaaaagaag tcaaataatg
     2461 tttattattt gacttcgaaa aaataaaaga aagctatgcg aattgagcgg gtttttgaag
     2521 gtattaatga tccatcgtat gcccaacagc accgcccaag agaccacctg ctgcagctcc
     2581 tatgcctgca ccaagagcac cgccgttaga tccgccaatg actgcaccgg ctacaccacc
     2641 aattgcagca ccggttgatt cgttccgact cataccagca caaccactta agaatacgca
     2701 gactaaagtc aagactatca ttttattacg catttttata aatcctatac ttataaacag
     2761 tatttaaaac accactccaa atgcggagct ccagatatga gacaccacaa aaaacaaaaa
     2821 gtttaaatgc tatattgata aaaagaacag aagataaatc catttcgcaa taggtcagcc
     2881 cttgggaata ttttttataa attagctata gttttgccaa ataatttttt tgttcgctat
     2941 ccttagatta atcgcattaa aaggaaggat caatgaaaat tttagtaacc ggtggacttg
     3001 gttttatcgg ctctcacatc gttgaatatc atttaaacaa aaatgatgcc gtttatgtcg
     3061 ttgataatct cagtacaggc agcctcgata atctgagtct gtttcaaaat aaccccgact
     3121 ttcaatttga taaggccgat atcctgacct ggcaaaacat ggaaaaagcg gtggcttggg
     3181 cagatcgcat ttaccatatg gctgcggttg tcggtaatct cagagtcata caagaaccta
     3241 ttaatgttat tgcgagtaac attgccggca cagaacggtt atttaatacc attaccaaaa
     3301 taggaaatac cccgagagtg atactggcat ccacatccga agtctatggt aaaagctcta
     3361 aatcaacgat gcaagaagag gatgatctta tcatcgaaaa ccctgtgcat ttacattcaa
     3421 attacgctat cagtaaacta agtgacgaaa ttattgggtt atcttatgat cgcatcaata
     3481 atatacccac cacaattatc cgttttttta ataccattgg tcccaggcaa acaggcactt
     3541 atggatttgt cgttccgaaa tttatcgcac aagcttgtaa gggcgaacct atctctatct
     3601 atggagatgg cagtcaaacc agaagctttg gcgatgttcg agatgttgtc gtggctttag
     3661 acttaattgc cgaaaatata aggacacgat acaatattct taatgtggga gtagatcatg
     3721 aaattacaat aaaagcctta gccaatcttg ttcgagaacg cgcaaacagt aagtcggata
     3781 ttatttatgt accctataaa aaaatttatg gaatggaatt tctagacatc atgcatcgaa
     3841 ggccggattt aacaaaactt cacaaactta ttcaattcaa gcacaaatgg actctcgaaa
     3901 aaaccgtcga taatctgatt ggtattttta atgaaaattc attaaaaccg caattgaaaa
     3961 ataaaaatct ttaaaatcgt ttagttaaca acgcttcgat agcgtatata cgatatttag
     4021 tgcctgagcc atacagcaga gagctactat tacgtgccgt cgcagaagca ctaatattta
     4081 agtaccaatc cgcgtaaata ccataaatag ccctcaaggc gatatctccc gcaaacccag
     4141 gtttcagcat tgtttcatct ttctgagctc ctgcgccaaa ggttaacaca taattaatgt
     4201 tctcattttg tttgatcgta aagtggctca gcgcttgata catttgataa gaacgttgat
     4261 cataataacc attcacaggg tcttgcaagg aaaagttttg ccacgttgca tagggaccaa
     4321 tatcaatatc aagatattga cttgctaaaa cagtcgcgtg cggataaaga gaaagcactt
     4381 tcagcgtgtt tttatctgaa aatttatcgt attcgaggcc aatatctaaa tatttttgtg
     4441 gatatggttg taaacttaca gtcattcgat tatatttttg taaagttttt aacgatactg
     4501 atcggggtga aaacgcgtac atatcttgtc cttttttaaa agctatt
//
LOCUS       DAORVT010000059         4533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_14073_length_4533_cov_3.072800, whole genome shotgun sequence.
ACCESSION   DAORVT010000059 DAORVT010000000
VERSION     DAORVT010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14073_length_4533_cov_3.072800"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PK389_01835"
     CDS             <1..396
                     /locus_tag="PK389_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005964976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HQY22486.1"
                     /translation="RVPGHSENRTTEISEISRSLKALARELNVPVVVLSQLNRGLEQR
                     PDKRPMMSDLRESGAIEQDADLIVFIYRDEVYNKDSNDNKGTAEIIIGKQRNGPIGSV
                     KLTFLGQFARFENYSADSRAMEGFYGART"
     gene            380..1459
                     /gene="alr"
                     /locus_tag="PK389_01840"
     CDS             380..1459
                     /gene="alr"
                     /locus_tag="PK389_01840"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005301951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HQY22487.1"
                     /translation="MARALRAFINVGALQQNLAKIRARVPHQKIMAMVKADGYGHGLV
                     NVAVALKDADAFGVACLEEAVVLRAAGVKNRIVLMSGFYDGSELPLILELNLELAIHH
                     ERQVHDLKMFKTGVETAIPIWIKINTGMNRLGFRCEQVRTVFETLSQCPLVKHQGFIT
                     HFASSEEVDNAETAAQIIRFFQTVETLPGEKSLANSAAILGIPASYADWVRPGILLFG
                     VSPYSGRVGRDEDLTAVMTLQSELIAIQSLKAGEAVGYGGEYVCKKDTRIGVVAGGYG
                     DGYPRLMPSGTPVLVNGRPVPLVGRVSMDMLTVDLGNQPEAKIGDPVQLWGPDLPVER
                     VAAAVGTSPYELLTRLAKRVPFEMC"
     gene            1609..1893
                     /locus_tag="PK389_01845"
     CDS             1609..1893
                     /locus_tag="PK389_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012981705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HQY22488.1"
                     /translation="MNIRPLHDRVIIERLDESVTPGGIFIPANASEKPQRGKVVAAGL
                     GKYENGVLRKLDVKVGDQVLFGKYSGTEVKVNGKDLLVMREEDIMGVLEG"
     gene            1957..3615
                     /gene="groL"
                     /locus_tag="PK389_01850"
     CDS             1957..3615
                     /gene="groL"
                     /locus_tag="PK389_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017846111.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HQY22489.1"
                     /translation="MAAKVLNFSGDARQRLLQGVTVLASAVKVTLGPRGRNAIIQKSF
                     GAPLVTKDGVTVAKEIELEDPIANMGAQMVKEVASKTSDVAGDGTTTATVLAEAIVKE
                     GQKLVAAGVNPMDLKRGIDKATTAVVAELKKLSVPCKDSKAIEQVGSISANSDHSVGK
                     LIADAMEKVGKDGVITVQEGATFTDELEFVDGMQFDRGYISPYFVNKQENMTCELENP
                     YILLVDKKISNIRELLPVLEATAKSGRALFIIAEDVEGEALATLVVNNMRGIVKVCAV
                     KAPGFGDRRKAMLQDIAILTKSKVISEEIGLTMEATTIEDLGSAKSIVVTKENTTIVG
                     GNAKSTEIESRIKELRQQVEDTTSDYDREKLQERIAKLSGGVAVIKIGAGSEVEMKEK
                     KARVEDALHATRAAVEEGVVPGGGVALVRTLPTLLKLKGDNEDQNHGITIIIRALEAP
                     LRQIVSNAGGEPAVVIERVKSESGNFGYNAASGEYGDMVKMGILDPTKVTRTALQQAA
                     SIAGLLITTECTISEAPKSEKESAGGMGGGMGGMGGMGGMGDMM"
     gene            3716..4456
                     /locus_tag="PK389_01855"
     CDS             3716..4456
                     /locus_tag="PK389_01855"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HQY22490.1"
                     /translation="MQKRILVWLILLLCFVQMPSFADDAEKKTDNSETKTIPKKQDLQ
                     HFDKINLSGVGNLYIKQTDQENFTMEADEALLPLIKVSVKDKTLYIDLADALSHLKAN
                     IKYYLNIKTVNSIIASGLTHIFIPNGLQTDALKLSISGFGSEAHININVKQLTAKVDG
                     GAKVEASGVAGAQMVEIHGAGEFDGTKLAGDIATVSIDGSSIAKTNISDKLSVDISGD
                     AELKYCAKPDITQKISGNGRISRLESGC"
BASE COUNT         1310 a          778 c         1167 g         1278 t
ORIGIN      
        1 cgggtgccag gacatagtga aaaccgcact actgagattt ccgagatttc acgctcttta
       61 aaagcgttag cacgcgagtt aaatgtgcca gtggtggtgt tatcgcagtt aaaccgagga
      121 ctcgaacaac gccctgataa acgtccgatg atgtctgatt taagggaatc gggtgcaatc
      181 gagcaagatg ccgatttaat tgtttttatt tatagagacg aagtttataa caaagatagc
      241 aatgataata aaggaactgc agaaatcatt attggcaagc aaagaaatgg tccgatcgga
      301 tccgtaaagt taaccttttt gggccagttt gcgcgttttg aaaattattc agcagatagt
      361 agagccatgg agggtttcta tggcgcgcgc acttagagcc ttcattaatg ttggagcgct
      421 tcaacagaat ttagcaaaaa ttcgtgcacg tgttcctcat caaaaaataa tggctatggt
      481 gaaggcggat ggttatggtc acggtcttgt aaacgtcgcc gttgctttaa aagatgctga
      541 tgcttttggc gttgcttgtc ttgaagaagc tgtagtattg cgtgcagcag gagttaaaaa
      601 ccgtattgta ctcatgagtg gtttttatga tggatctgaa ttaccactga ttcttgaatt
      661 aaatttagag cttgctattc accacgaaag acaagtgcat gatctcaaaa tgtttaagac
      721 aggtgtagag accgcaatac cgatttggat taagattaac acgggtatga atcgtttggg
      781 gtttcggtgt gagcaagtgc gtactgtttt tgaaacctta agtcaatgtc cgcttgtaaa
      841 acatcaaggt tttatcacgc attttgccag ttcagaagaa gtggataatg cggaaactgc
      901 agctcagatt attcggtttt ttcagacggt tgaaacgtta ccgggtgaaa aaagtttagc
      961 caattctgca gcgattttag ggataccggc ttcttatgca gattgggtgc gtcctggcat
     1021 tttactcttt ggcgtatcgc cgtattcggg tcgcgttggg cgagatgaag atctcactgc
     1081 tgtgatgacc ctccaatccg agctgattgc cattcaatcg ctgaaggcgg gagaggcggt
     1141 tggctatggt ggggaatatg tgtgtaaaaa ggacacccga atcggtgtcg tcgcaggggg
     1201 ctatggcgat gggtaccctc gtttgatgcc aagtggaaca ccggtgttgg tgaatgggcg
     1261 tccggtgcct ttagtagggc gagtttcgat ggatatgcta acagttgatt tagggaatca
     1321 gcctgaagcg aagattgggg atcccgttca gttatggggg cccgatttgc cggtcgaaag
     1381 ggttgccgca gcagtcggta caagtccata tgaattattg accaggctag ctaaacgtgt
     1441 gccttttgaa atgtgttaaa tttggtatac ttgccgtact gcccttgatt ttctggaagg
     1501 gagaccccac atacctggta gggatcgatt tatatgccga tccgctgggt tcgttgaaca
     1561 actgactttt tttaacgttg aatcggagga agagaaaaaa tgagcaatat gaatattcgt
     1621 cccttacatg atcgggtgat tatagaacgt ctagacgaaa gtgtaacgcc tggaggcatt
     1681 ttcatccctg ctaatgcaag tgaaaaacca caacgcggta aggttgttgc tgcaggtctt
     1741 ggaaaatatg aaaatggcgt tttgcggaag ctcgacgtga aagtggggga tcaagtgcta
     1801 ttcggtaagt attctggtac tgaagtaaag gttaatggta aagatctctt ggttatgcgt
     1861 gaagaagaca ttatgggtgt tctggaaggc tagagatttt atgcacaaga gtgcattttt
     1921 gaacaataaa caattgtata cagaggagta aataatatgg ctgctaaggt attaaatttt
     1981 agtggagatg cgcgtcagcg tttgttgcag ggtgttacgg tattagccag tgcggttaag
     2041 gtaacgcttg gaccccgtgg tcgaaacgcc attattcaaa aatcttttgg cgctccgctt
     2101 gttacaaaag atggcgtaac ggttgctaaa gaaattgaac ttgaagatcc cattgcgaat
     2161 atgggtgctc aaatggttaa agaagtggca tctaaaacat ctgatgttgc tggcgatggt
     2221 acaacaacgg caacggtatt agcggaagcc attgtaaaag aaggacaaaa acttgttgct
     2281 gccggtgtaa atccaatgga tcttaaacgt gggatcgata aagcaactac tgctgtggtt
     2341 gcagaactta aaaagctttc tgtaccttgc aaagatagca aagcgattga acaagtgggt
     2401 tctatttctg cgaactccga tcactccgtc ggtaagctaa ttgctgacgc gatggaaaaa
     2461 gtcggtaaag atggcgttat tacggttcaa gaaggtgcta cctttacgga tgaattggaa
     2521 tttgttgatg gtatgcagtt tgatcgcggt tatatctcac cttattttgt gaataagcaa
     2581 gaaaacatga cctgcgaatt agaaaatcct tacattttgt tagtggataa gaaaattagc
     2641 aacattcgtg aattgctacc tgtattggaa gcaactgcga aatcgggtcg cgctttattc
     2701 attattgctg aagatgtaga aggcgaagca ttagcaacat tggttgtgaa taatatgcgt
     2761 ggcatcgtta aagtctgtgc ggttaaagcg cctggttttg gtgatcgtcg taaagccatg
     2821 ttgcaggata ttgctattct gacaaaatct aaagtgattt cagaagaaat cggattaacg
     2881 atggaagcca cgacaatcga agatctgggt tctgctaaga gtattgttgt cactaaagaa
     2941 aacacaacca tcgttggtgg taatgctaag agcacagaaa ttgaatcgcg catcaaagaa
     3001 ttgcgtcagc aagtagaaga tactacttcc gattatgatc gcgagaagtt gcaagagcga
     3061 atcgctaaat tgtccggtgg cgttgcagtt atcaagatag gcgcaggctc tgaagtcgaa
     3121 atgaaagaga aaaaagcacg tgtagaagat gcattgcatg caacacgtgc cgctgttgaa
     3181 gaaggtgttg tgcctggcgg tggtgtggct ttagtacgca ctttgccgac gttgctgaaa
     3241 ttaaaaggtg ataatgaaga tcaaaatcat ggtattacga ttatcattcg ggcgctagaa
     3301 gcacctttac gtcaaatcgt ttcgaatgca ggtggcgaac ctgctgtggt tatcgaacgc
     3361 gttaaatcag aatccggtaa ctttggttac aatgcggcct ctggtgaata cggtgacatg
     3421 gtaaagatgg gtattttgga tcctaccaag gttacacgta ccgctttaca acaagcagct
     3481 tccattgccg gcttattaat tactacggaa tgtactatct cagaagcacc aaagtctgag
     3541 aaggaaagtg ctggtggtat gggcggcggc atgggtggca tgggcggaat gggtggtatg
     3601 ggcgatatga tgtaatatcg tctctatttc gcttaccttc tccggactct tatttgagag
     3661 tccggaagag ggagcccgca aggggccagt gagagctaga gttgagggaa gcgcaatgca
     3721 aaaacggatt ttagtttggc tgattctttt attatgtttt gtacaaatgc cttcttttgc
     3781 agatgatgca gaaaaaaaga cagataattc agaaacaaaa accatcccta aaaagcaaga
     3841 tctacagcat tttgataaga ttaatttgtc gggtgtcggc aatttatata ttaagcaaac
     3901 tgatcaagaa aattttacca tggaagcgga tgaagcgctg ttaccattga ttaaagttag
     3961 tgttaaagac aaaaccttat atattgatct cgcggatgct ttaagtcatc taaaagctaa
     4021 tatcaagtat tatttaaata ttaaaacagt caatagtatt atagcgtccg gtttaacgca
     4081 tatttttatt ccgaatggtc ttcaaacaga tgccctgaag ctttcgatta gtggattcgg
     4141 gagcgaagct catatcaata tcaatgtaaa gcaattgaca gctaaagttg acggtggggc
     4201 aaaggttgaa gcaagtggtg ttgcaggtgc gcaaatggtc gaaattcatg gagcaggaga
     4261 attcgatggc actaaacttg cgggagacat tgcgacagtt tccattgatg gatcaagcat
     4321 tgctaaaacc aatatctcgg ataagttgtc ggtagatatt tcaggtgatg cagagcttaa
     4381 gtattgtgct aagccagaca ttactcaaaa aatatccggc aatggtagaa tatcgcgttt
     4441 agagtcgggt tgttagcctc atcaatacgc cgcatttaac catatttgat ctggtttttt
     4501 aatttgtcgt ataaatagcg gatgtattca ttt
//
LOCUS       DAORVT010000060         4483 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_14319_length_4483_cov_3.810072, whole genome shotgun sequence.
ACCESSION   DAORVT010000060 DAORVT010000000
VERSION     DAORVT010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14319_length_4483_cov_3.810072"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PK389_01860"
     CDS             <1..552
                     /locus_tag="PK389_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HQY22491.1"
                     /translation="VKGYTPVASGICIALFDIGGFVGVLLAGWGSDKWFNQKRIPLIV
                     LSSFALVFAIGGLWYLEPQQIILTYFWVTLVGVLVYIPQMLVGLAAAECVTKSAAGTA
                     NGFVGFCAYLGVAASGYPLSIIIETWGWYGFIATLAICSALCVLMLLPIWSMKAGIYY
                     TENKETSAAPLESRIETETGATA"
     gene            complement(549..1841)
                     /gene="gltA"
                     /locus_tag="PK389_01865"
     CDS             complement(549..1841)
                     /gene="gltA"
                     /locus_tag="PK389_01865"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HQY22492.1"
                     /translation="MTNDKAELHVPGKEVISLPILSGTLGPNVMDVTSLNRFGIFTFD
                     PGFQSTAACKSKITFIDGDMGVLLHRGYPIAALAEESDYLEVCYLLLNGELPNKMQKD
                     NFINQIQHHTMLHEQFSSFFNGFPRNSHPMAIMCGAVGALSAFYHDSLDIKNINHRDL
                     AALRLIAKMPTIAAMAYKYSLGQPFVYPLNNLSYTENFLHMMFSIPSEPNKIDPILVR
                     AMNRIFTLHADHEQNASTSTVRLAGSSGANPFACISAGIAALWGPAHGGANEAVINML
                     QEIGDVSRIPTYLARAKDKKDTFRLMGFGHRVYKNFDPRAKVMRQTCYEVLEALKMHD
                     NPLFQLALKLEEIALQDPYFIEKKLYPNVDFYSGMILKAIGIPTNMFTVIFAMARTVG
                     WIANWNEMLSEPDLKIGRPRQLYTGPLERAFVPIEKRS"
     gene            2012..2401
                     /locus_tag="PK389_01870"
     CDS             2012..2401
                     /locus_tag="PK389_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22493.1"
                     /translation="MKRFVFLAVTLFLGLPVLAGVDGSSSQVVQESTSATKQSEKQDT
                     PEEKAYKAQMIKMDDAIAKKIAEIQKKQKEIETEVYLASTPPLVAERIILETQLRELQ
                     MARDKIQTQESSRKMTEDLKNSTSRSL"
     gene            2476..2847
                     /gene="sdhC"
                     /locus_tag="PK389_01875"
     CDS             2476..2847
                     /gene="sdhC"
                     /locus_tag="PK389_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015045452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HQY22494.1"
                     /translation="MKRRPINLNFFTIRFPVTAWVSVAHRLSGGFLFLMIPGLLWILQ
                     ESLASKTRFQSLGMFFSHPVAVIGLWLLLAALFYHWVVGLRHLLMDIHIGESKIGGRI
                     GAWLVICISLGFILLLGYKLW"
     gene            2841..3188
                     /gene="sdhD"
                     /locus_tag="PK389_01880"
     CDS             2841..3188
                     /gene="sdhD"
                     /locus_tag="PK389_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007017030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HQY22495.1"
                     /translation="MVEPAASLGRSGLQDWLIQRISAVILAIYTAFLVIYFVMHPILQ
                     YEQWHALFESKVMKYASALVLLSLLSHAWIGIWTVITDYVKPCALRLSIEIFFIIDLL
                     ICLMWGIRIIWSI"
     gene            3192..4483
                     /gene="sdhA"
                     /locus_tag="PK389_01885"
                     /pseudo
     CDS             3192..4483
                     /gene="sdhA"
                     /locus_tag="PK389_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205725.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
BASE COUNT         1230 a          795 c         1055 g         1403 t
ORIGIN      
        1 gtcaaaggtt atacgcctgt tgcatcgggt atttgtattg cgttgtttga tatcggtggg
       61 ttcgttggcg tgttattggc gggttgggga tcagataaat ggtttaatca aaagcgtatc
      121 cctttaattg tgttaagttc ttttgcattg gtttttgcga taggtggatt gtggtatttg
      181 gaaccgcaac aaattatatt gacgtatttt tgggtaacgt tagttggtgt attagtttat
      241 attcctcaaa tgttggttgg cttggcggca gcggaatgcg ttactaaaag cgctgcgggt
      301 actgcgaatg gttttgtagg gttttgcgct tatttaggcg tggctgcctc gggctaccct
      361 ttaagtatca ttattgagac ttggggttgg tatggcttta ttgccacctt ggcgatttgt
      421 tctgcgcttt gcgtattgat gttattaccc atttggtcga tgaaggcggg tatatattat
      481 accgaaaata aagaaacatc cgcagcacct ttagagagcc ggattgaaac cgagacagga
      541 gcaactgctt agcttctttt ttcgattggg acaaacgcgc gttctagagg tcctgtatag
      601 agttgacgtg gacgtcctat tttgagatcg ggttcactta gcatttcgtt ccaattggcg
      661 atccatccta ctgttctcgc catcgcaaaa atgacggtaa acatattcgt gggaatgcca
      721 atcgctttta aaatcatccc tgaataaaaa tctacattgg gatacagttt tttctcaata
      781 aagtaaggat cttgtagggc aatttcttcc agtttaagtg ctaattgaaa cagcgggtta
      841 tcgtgcattt ttaaggcttc taacacttcg taacaggttt gccgcatgac cttagcgcga
      901 ggatcaaaat ttttatatac tcgatgcccg aaacccatca atctaaacgt atctttttta
      961 tctttagcgc gtgcaagata cgttgggatc cgactgacat ctccaatttc ttgtagcata
     1021 ttgatgacag cttcgttggc gccaccgtgt gcaggacccc aaagcgctgc aataccggca
     1081 gagatacagg caaatggatt agctcctgaa gagcctgcaa gacgcactgt agaagtagac
     1141 gcattttgtt cgtgatctgc gtgtaatgta aaaatacgat tcatcgcacg tactaaaata
     1201 ggatcaatct tgttaggttc agaaggtata gagaacatca tatgtaagaa attttcggtg
     1261 taacttaaat tattcagagg gtatacgaag ggttggccca gcgaatattt ataagccatc
     1321 gctgcaatag taggcatttt agcaattaaa cgtaatgccg ctaagtctcg atgattgatg
     1381 tttttaatgt ccagagaatc atgatagaac gcggataaag caccgactgc accacacata
     1441 atagccattg gatgtgaatt acgtggaaac ccattgaaga aactactaaa ttgttcatgc
     1501 agcatcgtgt gatgttgaat ttggttaata aaattgtctt tttgcatttt gtttggtagc
     1561 tcaccattca agagcaagta acacacttcc agataatcag attcttctgc caatgcggct
     1621 atcgggtacc cacggtgtaa caaaacgccc atgtcgccat caataaacgt aattttggat
     1681 ttgcaggctg cggttgattg aaacccggga tcaaaggtaa atatgccaaa tcgattgaga
     1741 ctcgtgacgt ccatgacgtt gggtcctaat gtgccagaca agataggtaa ggaaatcacc
     1801 tctttaccgg ggacatggag ttctgcttta tcgttggtca tatggtattt cctattaaga
     1861 caatatctaa atattactct agtatagaaa aaattaaaca aaatttacaa aatttgcata
     1921 aaatcgcttc atgttcacaa gttctggact taacttattt ttatactata ctgaagccat
     1981 taagataaga ccatggcgtg agggaattgt gatgaaacgt tttgtttttt tagcggttac
     2041 acttttttta ggattgcccg tattggcggg tgttgatggc tcttcgtcac aagtggtgca
     2101 agaatctaca tcagcaacaa aacaatcaga aaaacaagat acacccgaag aaaaggctta
     2161 taaagcgcaa atgattaaaa tggacgatgc gattgccaaa aaaattgcag agatccaaaa
     2221 aaagcaaaaa gaaatcgaga ccgaagttta tctggcctct acaccgccat tggttgctga
     2281 gaggatcatt ttagagacgc aactccgtga attacaaatg gctcgagata aaatacaaac
     2341 gcaagaatcc tcaagaaaaa tgacagaaga tctgaaaaac agcacatcac gttcgctata
     2401 gagttaactg ttttggagtc ctaaataatt tagggctata ctctagttat agggttttgc
     2461 taaggaacaa ggattgtgaa acgtagacct atcaacctga atttttttac catacgtttt
     2521 cctgtgaccg cgtgggtttc tgtggctcac cggttatcgg gcggattcct ttttttaatg
     2581 atccctggct tgctctggat attgcaagaa tcgctggcat caaaaacgcg ttttcagagc
     2641 ttagggatgt ttttcagtca tcctgttgct gttataggtc tatggttact tttagccgca
     2701 cttttttatc attgggttgt ggggcttcgt catctattaa tggatattca tattggcgag
     2761 tcaaaaatcg gggggcgcat tggtgcttgg cttgtcattt gtatttcttt gggttttatt
     2821 ctgctgcttg gatataaatt atggtagagc ctgccgcctc tttaggacgt agtggtttac
     2881 aagattggtt aatacaacgt atttcagctg tcattttagc gatttatacg gctttcttgg
     2941 ttatttattt tgtgatgcat ccgatattac agtacgaaca atggcatgcg ttatttgaat
     3001 ctaaagtgat gaagtatgcc agtgcattgg tattgttaag cctcttgtcg cacgcgtgga
     3061 ttggtatatg gacggtcatt acagattatg ttaagccatg tgctctcaga ttatcgatag
     3121 aaatcttttt tatcatcgat ttattgattt gtttgatgtg gggaatacgc attatttgga
     3181 gcatataaga tatgacattg gctgaacaat cttttgatac gttaattatt ggtgccggtg
     3241 gtgctggcat gcgcgcggcg ctccaactgg ctgaacatgg aaaacgtatt gcggttattt
     3301 ctaaagtttt cccaacccga tcgcataccg tctctgccca aggtggtatt aatgcagcat
     3361 tgggtaatat gagcgaagat gattggcatt ggcatatgtt tgatacggta aaaggctcag
     3421 attatttagg agatcaagat tgcatagaat atatgtgtcg tgtgggtcct gaagttgtct
     3481 atgaattaga acatatggga ttgcctttct ctcgcttaga aaatggaaag atctatcaac
     3541 gtccgtttgg cggtcaatcc acacaatttg ggacgggtgt gcaagcagca cgtacttcag
     3601 cagcagcaga tagaacgggc catgcgttgt tacatacttt gtatcaacaa aatctaaaag
     3661 caaaaacaaa tttttttaac gaatggtatg ccttaaattt ggtgaaagat ttggatggca
     3721 gaattgcggg cgttgttgca ttatcgattg agacaggcgc acttttattt ttacgcggtc
     3781 ggtctgttgt attggcaacc ggtggcgcgg gtcggatcta ccaataaaca acgaatgcct
     3841 taattaatac tggcgatgga ttaggcatgt gtgttagggc aggatttcca ttgcaagata
     3901 tggaaatgtg gcaatttcat cccacaggat tgtctcatgt gggtgtatta atttcggaag
     3961 gttgtcgggg agaaggtggc tatctcatca ataaggatgg ccaacggttt atggaacgtt
     4021 acgcaccaca tgcaaaagat ctggcatcca gagatgttgt ttctcgcgct attgcggtgg
     4081 aaattcggga aggtcgtgga tgtggcaaag aaggtgatca tgttttatta aaactggatc
     4141 atttaggcaa agatcttatt ctagaacgcc tcccgggtat tcgcgaatta tcgatgacat
     4201 ttgctaatat tgatcccatc aaagatccca ttccggttgt accaacgtgt cattatatga
     4261 tgggaggtat tcctactaat aaatttggac aagtgctcac cgaaaataaa tctggagaac
     4321 atttaccggt tctcggttta tatgccgttg gcgaatgtgc gtgcgtttct gtgcatggtg
     4381 ctaatcgatt gggtggaaat tcattattag atttggtggt gtttggtcgt gctgctggat
     4441 tacatatttt ggaacatata aatgacgacg aattaaccgg aca
//
LOCUS       DAORVT010000061         4443 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_14565_length_4443_cov_3.425934, whole genome shotgun sequence.
ACCESSION   DAORVT010000061 DAORVT010000000
VERSION     DAORVT010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14565_length_4443_cov_3.425934"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..662)
                     /gene="pgsA"
                     /locus_tag="PK389_01890"
     CDS             complement(102..662)
                     /gene="pgsA"
                     /locus_tag="PK389_01890"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003291085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HQY22496.1"
                     /translation="MWRSIPNLLTLLRISLIPIFVLMFYLPFEWHYIASAIIFAIAGV
                     TDWLDGYLARKLHQVSPLGEFLDPVADKLIVAVALVLLVQSHASAWLAIPAAVIVGRE
                     IVISALREWMAELGKRQHVGVKGVGKFKTAAQMVAILLLLSEDPKLAGWITSFGYILI
                     YAAAFLTLSSMVVYLRLAWDDLIAEG"
     gene            complement(690..2504)
                     /gene="uvrC"
                     /locus_tag="PK389_01895"
     CDS             complement(690..2504)
                     /gene="uvrC"
                     /locus_tag="PK389_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007146356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HQY22497.1"
                     /translation="MSALEPFLANLTTLPGVYAMLDAKGVVLYVGKAKNLKKRVTTYF
                     RRALDTKTQQLVKQIKSIEVTVTRNEREALLLENNLIKELQPRYNIIFRDDKSYPYLK
                     LTVDAVFPRLSFHRGPKGEGKVYGPYPSVREARVALNLLQSIFRLRQCDEVFFRNRTR
                     PCLQYQIKRCSAPCVGYITAENYKKEVELARLFLEGKNKNVIEKLIHQMTEASSEKAF
                     EQAARIRDQIALLRTIQEHQIIMGNMKDVDVLGIYAEGNIACVHVLVIREGRMLGSRQ
                     YFPNKTALILPEEDMLQTLLEAFILQHYKMDIPKTILLPFALKNRESVSVLLGERRLG
                     SVNIATAVKGDRLRWVKMALTSARLALISRVKQTRDLTPRFLALQESLQLEFIPEKLV
                     CFDASHTLGEATVVSCVVFDTHGPSKPEYRRFNIRAKTGGDDYAALKEGLTRHFTRLK
                     SEGKTLPDVLLVDGGKGQLNIAKAVLEECQVFGVVLLAVAKGPERKAGLETVYLSSND
                     KVIQLQLNAAALLLIQQIRDEAHRFAITAHRKRIAHSRSTSTLERVPGMGSKRCQSLL
                     QYFGGLPEILQASIEELAKAPGISQGLAGRLYEILHGQ"
     gene            complement(2593..3237)
                     /gene="uvrY"
                     /locus_tag="PK389_01900"
     CDS             complement(2593..3237)
                     /gene="uvrY"
                     /locus_tag="PK389_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018298674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HQY22498.1"
                     /translation="MINVLVVDDHDLVRSGIVRLLSDIAGITVVGEANTGEEAVKIAR
                     EKRPNVVLMDIRMPGIGGLEATRKIVHQNPEIKVIALTIYENEPFPTKLLQAGASGYL
                     TKGALIDEMVLAIRTVYAGNRYLGPEIAQQMALKSVAENEQSPFEQLSERELQVMIMI
                     TAGQSVQTIAEKLCVTPKTINSYRYRIFEKLNVQSDVELTLLAMRHGILETETT"
     gene            complement(3294..3382)
                     /locus_tag="PK389_01905"
     tRNA            complement(3294..3382)
                     /locus_tag="PK389_01905"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3346..3348),aa:Ser,seq:tga)
     gene            3567..4244
                     /locus_tag="PK389_01910"
     CDS             3567..4244
                     /locus_tag="PK389_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020565193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HQY22499.1"
                     /translation="MQPNIDTVRHAQSGMVIQTNSVIRNTYILLSMTLLFSAFTAWLA
                     MLTNATPMGLMVLPIYFGLLFLTQSLRNSVWGIASIFAFTGFMGYTLGPILNFAIQGF
                     SNGTQIIMASLGLTGLIFFALSGYALTTRKDFSYLGGFLFVAATIGMMASIVGLFFPV
                     PLLYLGVSSAFVLIASGTILFQTSQIIHDGERNYIMATIALYVSIYNLFISLLQLFML
                     LAGRRNN"
BASE COUNT         1270 a         1072 c          810 g         1291 t
ORIGIN      
        1 aactcgcgac cccaaccttg gcaaggttgt gctctaccac tgagctattc ccgcactaag
       61 gatgcattct atcgaaacat gcgtgcgcat tcaagctctg attatccttc agcgataaga
      121 tcgtcccaag cgaggcgcag atacacaacc atagacgaaa gcgttaggaa tgcggcggca
      181 tagatgagga tatacccaaa agaagtaatc caacctgcta attttggatc ctcagaaagc
      241 aacaaaagaa tcgcgaccat ttgcgccgcc gtcttaaact tgccaacccc cttcacaccc
      301 acatgctgac gcttgcctaa ttctgccatc cactctcgca gcgcagaaat aacaatttca
      361 cgtccaacaa taacggcagc gggaatcgct agccacgcag aggcatgcga ttgtaccaat
      421 aacactaatg caacagcgac aattaattta tctgcaacag gatctaaaaa ttcccctagc
      481 ggtgacactt gatgaagttt tctggctaaa tagccatcta gccaatctgt aacgcctgcg
      541 attgcaaaaa taatggctga agcaatatag tgccattcaa agggcaggta aaacatcaaa
      601 acaaaaatag gaattaaaga aatccttaac agggttaata aattcggtat acttcgccac
      661 ataaggttga ttctcgctta ttaaataaat tattgtccat gtaaaatttc ataaagacgc
      721 cctgctaatc cttgactaat ccctggtgcc ttagctaact cttcaatgct cgcctgcaaa
      781 atttcgggca accccccaaa atactgtaaa agactttgac aacgcttgct acccatgccc
      841 ggcaccctct ctagtgtaga cgtgcttctg ctatgcgcaa tgcgcttgcg gtgcgcagta
      901 atcgcaaaac gatgtgcctc atcgcgtatc tgctgaatta aaagtaaggc agcggcattg
      961 agctgcaact gaataacttt gtcatttgaa gacagataaa ctgtctcaag ccccgctttt
     1021 ctttcgggac ccttggcaac cgccaacaaa acaaccccaa aaacctgaca ttcctctaaa
     1081 accgcttttg caatattaag ctgaccctta cctccatcca ctaacagcac atcaggcaaa
     1141 gttttaccct ctgattttaa ccgggtgaaa tgccgagtca acccctcttt taaagccgca
     1201 taatcatcgc cccccgtttt tgcccgaata ttaaaccgtc gatactccgg tttactaggc
     1261 ccatgagtat caaataccac acaagatacc actgtggctt ctcctaaggt atggctagca
     1321 tcaaaacaca ctaatttttc tggaataaat tctaattgca gagattcctg taaagccaaa
     1381 aatcggggag tcaaatcacg agtttgtttt acgcgactga ttaatgccaa tctcgcgctc
     1441 gtaagcgcca ttttaaccca acgcaaacga tctcctttaa ccgctgttgc gatattaaca
     1501 gaccccaatc gccgttcacc caaaagtacc gacacactct ctctattttt taatgcaaac
     1561 ggtaacaaaa ttgttttggg aatatccatt ttataatgtt gcaaaataaa agcttctaac
     1621 agggtttgca acatatcttc ttctggcaag atcaatgctg ttttattcgg aaaatattgc
     1681 cgactgccca gcattcgccc ttctcgtatc acaagcacat gtacacacgc aatattacct
     1741 tcggcataaa tacccaaaac atcgacatcc ttcatattac ccataataat ttggtgttct
     1801 tgaatggttc taagcaaggc aatttgatcg cgaattcgag cagcttgttc aaaggctttt
     1861 tcgctactcg cctctgtcat ctgatgaatt agtttttcaa taacgttttt attttttccc
     1921 tctaaaaaca atcgagcaag ctcaacctct tttttataat tttctgcggt aatataccct
     1981 acacaaggtg cagaacagcg ttttatttga tattgcaaac aaggacgcgt tcgatttcta
     2041 aaaaaaactt catcgcattg acgcaaccga aaaatactct gtaataaatt taaggcgact
     2101 cgtgcttcac gcacactcgg ataaggacca taaactttac cctccccttt cggaccccgg
     2161 tgaaaagaaa gtcgcggaaa tactgcatcc actgttagtt taagataagg ataagactta
     2221 tcatctcgaa aaataatatt ataacgcggc tgcaattctt taatgagatt attttctaat
     2281 aataatgcct cgcgttcatt gcgtgtaacg gtcacttcta tggatttaat ctgtttaacc
     2341 agctgttgtg ttttagtatc taaggcacgc cgaaaatatg tagtaactct ttttttaaga
     2401 ttttttgctt ttccgacgta aagcacaaca cccttagcat ctaacatagc ataaacaccc
     2461 ggaagggttg ttaaattggc taaaaaaggt tctagtgcac tcatagcttt tcagagactt
     2521 gttttggtaa caggatccat gcttgagcga agcgagttca gcagcaaatc aaaataagca
     2581 tctgaaaagc tatcaggtcg tctccgtctc taaaatacca tgccgcatag ctagcaaagt
     2641 taattctaca tcgctttgta catttaattt ttcgaatata cggtatcgat agctgttaat
     2701 ggttttaggc gtcacacaca atttttcggc aatcgtctga acactttgcc cagcggttat
     2761 cataatcatc acttgcaatt cacgttcaga aagttgttca aacggagact gctcgttttc
     2821 ggcaaccgat ttaagcgcca tctgttgggc aatttccggg cctaagtaac gattacccgc
     2881 atagacagtc cgaatcgcca aaaccatctc atctatcaaa gcaccctttg ttaaataacc
     2941 actcgcaccc gcttgcagca attttgtcgg aaacggttcg ttctcataga ttgttaaggc
     3001 aataacctta atttctggat tctgatgaac tattttgcgt gtcgcttcca gtcctccaat
     3061 acccggcatt ctgatatcca ttaatacaac attcggacgc ttttcccgag caattttaac
     3121 cgcttcttct cctgtgttcg cctcgccaac gactgttatt ccagcaatat ccgacagcaa
     3181 tcgaacaata cccgaccgca ccaaatcatg gtcatctact accagtacat tgatcacgct
     3241 taggatctcc tgtgtttgca ttaagactgt aactgctatc agctcgtcaa ggctggcgga
     3301 gaggcaggga ttcgaaccct gggaggggga aaccctcaac ggttttcaag accgccgctt
     3361 taaaccgctc agccacctct cctgaacgag cattataatc aagcatactc taaaagacga
     3421 gagacttatt gattttgtta gttttctaaa cttatatttg cgtgcattta atcttatctc
     3481 taagagattt atttgaaggg ctccctgtaa aaaacacgaa cttgtggtac aattcgagac
     3541 agagagagat ttaggagata aaggatatgc aacccaatat tgataccgtc cgacatgctc
     3601 agtctgggat ggtcatacaa actaattccg ttattcgaaa tacctacata ctgctttcga
     3661 tgacactact ttttagcgca ttcacggctt ggcttgctat gctaaccaat gccaccccca
     3721 tgggtttaat ggtattgcct atctattttg gactattgtt cctcactcaa tctcttcgaa
     3781 atagcgtatg gggcatcgct tccatctttg cctttacggg atttatgggc tacacattag
     3841 ggcccatctt gaattttgcg atccaggggt tcagtaatgg tacgcaaata attatggcct
     3901 ctttggggtt aaccgggctt atcttcttcg ctttatccgg atatgcactc actacgcgta
     3961 aagactttag ctacttaggg ggatttttgt ttgtagcggc aacaataggg atgatggcca
     4021 gcattgttgg tctcttcttt cctgtacctt tgctctatct tggtgtctcg tctgcttttg
     4081 tattaattgc ttccggcacg attttattcc aaaccagcca gatcattcat gatggcgaac
     4141 gcaactatat tatggcgacg attgcgttat atgtgtctat ttacaacctg ttcattagcc
     4201 ttttgcagct gtttatgctg ttggctggcc gtcgcaacaa ctaattctcc tagggttaac
     4261 tccccttcaa aagaacttca tccctcctca tggagggatt ttttttattc aacatttaaa
     4321 aaaattaagc taactttcct tcttctaagg tgtttgacgc acgttaggat ccgttattga
     4381 ctcacattaa aaaatcagac atactttcag acagagtttt taaggtagag ggctcaaatg
     4441 cat
//
LOCUS       DAORVT010000062         4435 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_14594_length_4435_cov_3.663014, whole genome shotgun sequence.
ACCESSION   DAORVT010000062 DAORVT010000000
VERSION     DAORVT010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14594_length_4435_cov_3.663014"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..1523
                     /locus_tag="PK389_01915"
     CDS             183..1523
                     /locus_tag="PK389_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22500.1"
                     /translation="MDTIGNIHTKSWNTLLFFILSALVISSIAVLFWPGYMSPDSIGQ
                     FNQSLANNYSDHHPPMMSFCWKMFNYIYTGSQVYFLFQLLLLFSSVCIFQTIVKESWL
                     KFAIALLPFVPNVAAYSGAIWKDISFAYAFLLAGMLLTKSNILSQKLTLPKIILILLL
                     LIYGVGVKYQAQFVLPVMGLWLGLTLTDSKLNVKAILIGVATTIFVIFFVFLFNKSLV
                     HVEKEQHAWQMVKLYDLAGISVRVGKNLLPEFVTSQENFSMDSVKKLYSPERVDELMK
                     DWYPGAPLSRAKDEKERNLLWSTWMSAVVHHPIAYFSHRFSLWFNMVSKSPIKSVNQL
                     SNFDNVPAKTKFVLRHVGDTFFSLIRELSRFVLMLPFVFAYLFWGGYLYKKNSPYGLA
                     LLMMNLAGLSLLLALFIFSMAADLRYIYLSTCFLFFSHPIAFLALRALLPGAPR"
     gene            1783..1896
                     /locus_tag="PK389_01920"
                     /pseudo
     CDS             1783..1896
                     /locus_tag="PK389_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002840721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
     gene            1889..2593
                     /locus_tag="PK389_01925"
     CDS             1889..2593
                     /locus_tag="PK389_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22501.1"
                     /translation="MSEAVVSESVSEFDELCQAYITELYRNVSGFISYQPGEKETAQL
                     EKVFLTYGEILFPSFNKVIQRMDINENDVFYDLGSGAGKSALQFFLKTPVKKVIGIEA
                     NKLRSDSCLKIYKQVKREFPELFEGDRILKCLPSNFLEVDISDATIIYICSTCFNTEL
                     LSDIGKIVDKCPNIKYLISIPSVTYDYASIPCKLPIEEVVDIECSWGHTKFYLYTLSP
                     TSPVDPATLPSYFVKK"
     gene            2648..4000
                     /locus_tag="PK389_01930"
     CDS             2648..4000
                     /locus_tag="PK389_01930"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY22502.1"
                     /translation="MIKSKFNNWISTYLNRRMLVVFMLGFSSGLPLALIGGTLQAWFK
                     TAGASMILIGFTTLIGQPYSYKYLWAPLLDKYAPTAMVDRRRCWMFMTQLLIIGAIIA
                     MAMCNPQAMVHVFKWDVPVLFLLGLFLSMCSATQDTAIDAYRIEILKHDERGLGAALG
                     IEGYRLAMIASGGFALILADKIGWQATYLWMAGLMSIGVIATAIAPPVQQVEKSVGSL
                     PALIVISFKDFLLRKKAWLILLLIVLYKLGDAFSHALSSTFLLDLDFSLTEVGMINKV
                     LGVVATLIGIFVGGLFMTRVGLYRALVYFGILAALTNLTYMVLALVGKNFELACGAVF
                     IENICAGMGTAAFIALIMSLCNKNYTATQFALLSSLTALGRTYVGPVSGYLVQTYGWA
                     IFYGFSAAVAIPGLLLLIYLRKEIERRDVDSTAESVLVESSTRDTVVNEVSQFSSVKV
                     "
     gene            4254..>4435
                     /locus_tag="PK389_01935"
     CDS             4254..>4435
                     /locus_tag="PK389_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22503.1"
                     /translation="MDRELRVDIYNYFSIEILENIGIEKPNQTQIDKVENALKKTIKA
                     LNNKLTNGSKSRMDFL"
BASE COUNT         1253 a          729 c          939 g         1514 t
ORIGIN      
        1 tatatacatt tttaaccttt ttttaagtgt tttttggttt tgttttgttt ggctatgatt
       61 aaagtattca cattgctatc caaatttgat tccggttgct tatattgtta ctatgtgtca
      121 ctcaaaaggt tttagattta aaatatggga aactaacttt tagtgttcgc ggtgagaaat
      181 gcatggatac aataggcaat atacacacta aatcttggaa tacgctttta ttttttattt
      241 taagtgcttt agttatcagc agtattgctg ttttattttg gcctggatat atgtctccag
      301 atagcattgg gcaattcaac caaagtttag caaacaatta ttcagatcac cacccaccga
      361 tgatgtcatt ctgttggaaa atgtttaact atatttatac gggatctcaa gtatattttc
      421 tgtttcagct tttgcttctt ttttcctctg tttgcatttt tcaaacgatt gttaaagagt
      481 cgtggttaaa attcgctata gcactgcttc cttttgttcc aaatgttgct gcatatagtg
      541 gagcgatatg gaaagatatt agttttgcat atgcatttct gcttgcgggg atgttgctta
      601 caaaatcaaa tatactttca caaaaactga cacttcccaa aattattctc attttgttgc
      661 tgttgatata tggggttggt gttaaatatc aagcacaatt tgttctgcct gtgatgggcc
      721 tttggttagg gttaacgctt accgattcaa aattaaatgt caaagcaata ttgataggcg
      781 ttgccacaac aatctttgtt atttttttcg tatttctttt taataaatca ttagtgcatg
      841 tagaaaaaga gcagcacgct tggcaaatgg taaaacttta tgatttagca gggatcagtg
      901 tgagggtggg gaagaattta ttgcctgaat ttgtgacatc gcaagagaac ttttctatgg
      961 atagtgttaa aaaattatat tctcctgaaa gagtagatga attgatgaaa gactggtatc
     1021 caggggctcc tctttcacga gccaaggatg aaaaagaaag aaacttgtta tggtccacat
     1081 ggatgagtgc ggttgtccat catcctatcg cttatttttc tcaccgtttt tctctgtggt
     1141 ttaatatggt ttctaaaagc ccaataaaat cagtaaatca gctgtcaaat tttgataatg
     1201 tccctgcaaa aactaaattc gtgttgcgac acgtcggtga tacatttttc tcgttgatac
     1261 gagaactctc tcgttttgtg ttgatgcttc cctttgtatt tgcatatctt ttctgggggg
     1321 ggtatttata caagaaaaat agcccttatg ggcttgcact cttaatgatg aatcttgcag
     1381 ggctatcttt gttgttagcc ctctttattt tttctatggc ggctgatttg aggtacattt
     1441 atttaagtac gtgttttctt ttcttttcgc atccgattgc gttccttgca ctgcgtgcat
     1501 tgttgccagg agcaccgcga taatctatta ttaaacaaaa gttacctcta aagcactctg
     1561 tacaaatatt ggagttgtat tataataacc gaaggtgtag attcataagt gacaaggatg
     1621 acttcttagg attgttttta aaagactgta ttctcaatta actaggttta aaaaatgagc
     1681 aataaagagt ttgagccgcg tgccgctgtt atcgaaaaaa taaggatgct tgtggcgcat
     1741 aaaaagaaag cgcgtggatt aagtcaatca cctgcctcac ccgtatctgt gcctgtcgtg
     1801 ataggtgcca aaacaggcag gaatgatcct tgtccttgtg ggagtgggaa aaaatacaaa
     1861 aaatgctgtg cgttaaaagg agcttctgat gagtgaggcg gtagtgagtg aatcggtttc
     1921 agaatttgat gaattatgtc aagcgtacat tacagaactg tatcgcaatg tctccggttt
     1981 tattagttac caaccgggcg aaaaagaaac tgcgcaactc gaaaaagtgt ttttaaccta
     2041 cggtgaaatt ctttttccaa gctttaataa agtgatacag cgcatggata ttaatgaaaa
     2101 tgatgtcttt tatgatttag gttcgggggc cggaaaatca gcattacaat ttttcttgaa
     2161 aacgcctgtt aaaaaagtca tcggtatcga agccaataaa ctacggagtg actcatgctt
     2221 aaaaatatat aagcaggtta aaagagaatt tccagaattg tttgaaggtg atagaatact
     2281 aaaatgcttg ccgtctaatt ttttggaagt ggacatcagt gatgccacaa tcatttatat
     2341 ctgttctact tgctttaata cagagctttt gtcagatatc ggcaagatag ttgataaatg
     2401 ccctaatatt aaatacctga ttagtattcc gagtgttact tacgattatg caagtatccc
     2461 gtgcaaattg cctattgaag aagtcgtgga tattgaatgc tcttgggggc atactaaatt
     2521 ttatttgtat acgctttcgc ctacaagccc agttgatcca gcaacactcc cgtcttattt
     2581 cgttaagaaa taatgccttt aatttttggc ttcgctgctg caatttgtat ccgatcactg
     2641 gatattcatg ataaaaagta aatttaataa ctggatttct acctacttaa acaggcgcat
     2701 gttagtcgtt tttatgttgg gtttttcgtc cggcttacct ttggccttga ttggtggaac
     2761 tttacaagcg tggtttaaga ctgctggtgc tagcatgatc ttgattggtt tcactacttt
     2821 aattggtcaa ccgtattcct acaaatacct ttgggcaccg ctgttagata aatatgcacc
     2881 cactgcaatg gtcgatcgtc gacggtgttg gatgttcatg acgcagctat tgattattgg
     2941 tgcaatcatt gcgatggcaa tgtgtaaccc gcaagcgatg gttcatgtat ttaaatggga
     3001 tgttcccgta ttgttcttgt tgggtttatt tttgagcatg tgctcagcaa cccaagatac
     3061 cgctattgat gcttaccgta tcgaaatttt aaaacatgac gaacgcggtc tcggtgcagc
     3121 gctcggtata gagggttata gattagcgat gatcgcctct ggcggttttg ctttaatact
     3181 ggcggataaa ataggttggc aagcaacgta tttatggatg gcaggattaa tgtcaatcgg
     3241 tgtgattgcc actgcgattg cgcccccggt gcagcaagtc gaaaagtctg ttggtagttt
     3301 gccggctttg attgttattt cgtttaaaga ctttttgttg cgtaaaaaag catggttaat
     3361 tttattgtta attgtattgt ataaactcgg agatgccttc tcacatgcat tatccagtac
     3421 atttttgtta gatctagact tcagtctgac tgaagtcggt atgattaata aagtattggg
     3481 tgtggttgct actttaattg gtatctttgt gggcggctta tttatgactc gagtgggctt
     3541 gtatcgtgca ttagtatatt ttggtatttt agcggccctt acgaatttaa cctacatggt
     3601 actcgcgtta gtcggtaaga attttgaact cgcctgtggt gcagtcttta ttgaaaacat
     3661 ctgtgctggt atgggtacag cagcatttat tgctttaatt atgagtcttt gtaacaagaa
     3721 ttataccgcg acgcagtttg cgttattgtc ttctttaacg gctttaggac gaacgtatgt
     3781 ggggcccgtt tcgggttatc tggtacaaac atatggttgg gcaatatttt atgggttttc
     3841 tgcagctgtg gcgattccgg gcttgttgtt attaatctat ttgcgcaaag aaattgaaag
     3901 acgtgatgtc gatagcactg cagaatcggt acttgtggaa agttcgacac gagataccgt
     3961 tgtaaatgag gtatcgcaat tcagcagtgt caaagtgtga gaaaaaaggt ttttttctaa
     4021 agtttagaac gattagcagt gaacttagtc gttgtactgt attaaataaa acatctggaa
     4081 aatagtggag cagtgcaaat ctaggcatac cgcgtactat aacgaattac ttgtatgata
     4141 caccctcgtc gcatctaaag aattattgat aataaatagt tattacgata ttatttgtcc
     4201 gtaaagcaag ttataattgt cccattaaga ttaacactga caaggagcgt actatggatc
     4261 gagagttacg agtagatatc tacaattatt tctcaataga gatattggaa aatataggta
     4321 tagaaaaacc taatcaaact caaatcgata aagtagaaaa cgcacttaaa aaaacaataa
     4381 aagctttaaa caacaagctc actaatggat cgaaatcgcg gatggatttt ttgat
//
LOCUS       DAORVT010000063         4340 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_15193_length_4340_cov_2.403267, whole genome shotgun sequence.
ACCESSION   DAORVT010000063 DAORVT010000000
VERSION     DAORVT010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15193_length_4340_cov_2.403267"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..1198
                     /gene="murG"
                     /locus_tag="PK389_01940"
     CDS             98..1198
                     /gene="murG"
                     /locus_tag="PK389_01940"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HQY22504.1"
                     /translation="MTKQRILIMAGGTGGHVFPALAVAERLLSQGWDIHWLGTRAGLE
                     ASIVPKANIAIHYVSVKGLRKNGILGWCLAPLRLLWALFQSLCVIHQLKPDVVLGMGG
                     FISGPGGLAAWLLRCPLVVHEQNAIVGLTNRALSHLANRVLEAFPGAFNAKVKAIYTG
                     NPIREVLLAAPAPTLRFTERRLKEPTEPLRLLVLGGSQGAVALNEVCPLAIQKMPIEK
                     RPVVWHQTGVKNEAVTKTKVAYDSAGISARVEPFIEDMAAAYGWADLVLCRSGALTVT
                     ELATVGVGSILVPYPFAVDNHQTANGRFLEKADAAKIIDQSCLSAENLAGLLLELFSD
                     NDRLFKMAEAAFSVAKRDALTEVVRYCENTHL"
     gene            1442..2854
                     /gene="murC"
                     /locus_tag="PK389_01945"
     CDS             1442..2854
                     /gene="murC"
                     /locus_tag="PK389_01945"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005332111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HQY22505.1"
                     /translation="MEQKNQELNMRRIRRIHLVGIGGAGMGGIAEVLLNLGYTVSGSD
                     PSKNTMVDHLKKLGIRVSSTHDAKNVVDADVVVVSSAVSQQNVELVCARERHIPIVQR
                     ATMLGELMRFKEGIAIAGTHGKTTTTSLITSLMAEGGLDPTFVIGGRLNSLGRNAKLG
                     AGRYFIAEADESDASFLQLMPMVAVVTNIDRDHLENYQQDFSILKKSYLQFLHRLPFY
                     GLAVVCLDDREIQTILEEIARPLITYGFSQNADVRAIDFIQKVNQSEFTVLRKSKPPL
                     MITLNLPGQHNVLNALAAIAVATEEGISDEAIQRGLLKFEGVGRRFQLLGEITHHNKK
                     ALMIDDYGHHPREIQVVLEALRAGWNDRRLVMVFQPHRYTRTKALFEDFVSILSLVDV
                     LILLDVYSAGEVAIAGADSRTLAFSIRSRGRVDPIFIADKRELSGVLATVLEDNDVLL
                     MQGAGDISALATELAVNAKK"
     gene            2867..3796
                     /gene="murB"
                     /locus_tag="PK389_01950"
     CDS             2867..3796
                     /gene="murB"
                     /locus_tag="PK389_01950"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012570461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HQY22506.1"
                     /translation="MRGLLKFNEPLAMHTSWHIGGPADKYFRPADTKDLAEFLKTSVK
                     DNEPITWLGLGSNVLISDSGIRGVVIHTLAQAGAGAITVSEPESNTEKVLIWVDAGVT
                     CAKLAKYCAKEGLLGGEFFAGIPGTLGGALAMNAGAWGGETWRLVKQVEVINRYGEQT
                     IRSSNMYKIKYRSVERIRDNFQNNVPEKLHEEWFIAGCFEFERGDSAIATRKVKALLQ
                     ERTAKQPIGVFSCGSVFKNPEGNHAGKLIEASQLKGFTIGGAQISPKHANFILNLGYA
                     KAQDVLALIKHIQAVVLKDHQICLEPEVRILGF"
     gene            3990..>4340
                     /locus_tag="PK389_01955"
     CDS             3990..>4340
                     /locus_tag="PK389_01955"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HQY22507.1"
                     /translation="MSVSRMPMVSIFLPLAIILVSFLSLVVWSRHSDRFPLRTVEIRS
                     SLHRVQETEIQASVLPFFDKGFFWLNVQAVQACLKRIPWVQTADIRRVWPDRLLVSVQ
                     EKTAQAKWGEKGVLS"
BASE COUNT         1209 a          734 c         1119 g         1278 t
ORIGIN      
        1 ggttgtggga ttattagccg ttggcatttt gctccgcatt gaatttgaat tgtcgaataa
       61 aaggcaaaaa gcttaataag ttgttagaat gatcagcatg actaaacagc gtattttaat
      121 tatggcgggt ggaacaggtg ggcatgtgtt cccggcgctt gctgttgccg aacgcttgct
      181 aagccaaggg tgggatatac actggcttgg cacaagagcc ggtctagaag cgagcattgt
      241 acccaaagcc aatattgcga ttcattatgt ttcggtaaag ggccttagaa aaaacggtat
      301 cttaggctgg tgcttagcac ctctcaggtt actatgggcg ctttttcaat ccttatgcgt
      361 tattcatcaa ctaaaaccag atgtcgtgtt aggcatgggc gggtttatct ccggtcccgg
      421 tggccttgcg gcttggttgt tgcgatgtcc tttagttgtt catgaacaaa acgccattgt
      481 gggtctgaca aatcgtgcat tgtcacattt ggccaatcgt gtgctagaag ctttcccggg
      541 tgcttttaat gcaaaagtga aagcgattta tacgggcaat cccattcgtg aggtgctttt
      601 agcagcacct gcgccaaccc tgcgttttac agaacgtcgg ttgaaagaac cgacagaacc
      661 cttacggctg ttagtgttgg gtgggagtca gggtgccgtt gccttaaatg aagtttgccc
      721 tctggctatc caaaaaatgc cgattgaaaa gcgaccggta gtgtggcatc aaacaggcgt
      781 taaaaacgag gccgttacaa aaacaaaagt ggcgtacgat agcgcaggga tttcagcacg
      841 ggtcgaacct tttattgagg atatggcagc tgcttatgga tgggcagatc tggttttatg
      901 tcgatcgggt gcgctgacgg tgacagaatt agcaacggtg ggtgttggta gtattttagt
      961 gccgtatccc tttgcggtgg ataatcatca aacagcgaat ggacgttttt tagaaaaggc
     1021 ggacgcggca aaaataatag atcaatcatg cttaagtgcc gaaaatttag ctggattatt
     1081 actggaatta ttttcggata atgatcggtt attcaaaatg gcagaagcgg cttttagtgt
     1141 tgcaaaacga gatgcgttaa cggaagtggt aaggtattgt gagaatacac atctctaaga
     1201 ttcgataatt ttcttagtcc aattatacat tgtttctata atctaacgaa cttctatttg
     1261 tctcgtttta accagtatgt gtagccttaa tacacttgct ggttaaaatc aaacattttc
     1321 aataaaatcc ttccagttta ttactcgcta aaatgctaaa ctggcgttct tagatcaggc
     1381 atactaaact gttagaatga ccagtatgac taaatggcgt attttagaga gggtgaaagg
     1441 catggaacaa aaaaatcagg aattaaatat gcggcgtatt cgtcgaatcc atttggtagg
     1501 tattggtggc gctggcatgg gtggcattgc tgaagtctta ttaaatttag ggtatacggt
     1561 gagtggttct gatccctcaa aaaacaccat ggttgatcat cttaaaaaat tgggcattcg
     1621 tgtttcgagt acgcatgatg ctaaaaatgt agtagatgcg gatgttgtgg tggtgtctag
     1681 cgcggtctct caacaaaatg tcgaattagt gtgcgcgcgt gagcggcata ttcccattgt
     1741 gcaacgcgcg accatgttag gtgagttaat gcgttttaaa gaaggtattg cgattgcggg
     1801 tacgcatgga aaaaccacga ccaccagcct gattacaagt ttaatggcgg aaggtggact
     1861 tgatccgact tttgtaattg gtggacgact gaatagctta ggaagaaatg ccaaattagg
     1921 tgcggggcgt tattttattg cagaagctga cgaaagtgat gcttcttttt tgcagctgat
     1981 gccgatggtt gctgtggtaa ccaatattga tcgagatcat ttagaaaatt atcaacagga
     2041 tttttcgatt ttaaaaaaat cgtacttgca atttttgcat cgcttgcctt tttatggttt
     2101 ggcagttgtt tgcttggacg atcgggaaat tcaaaccatt ttagaggaaa ttgcgagacc
     2161 gttaattact tacggttttt cacaaaacgc agatgtgcgt gccattgatt ttatacaaaa
     2221 agtgaatcaa tcggaattta ctgtgttgag aaagagcaag cctcctttga tgatcacttt
     2281 gaatttaccc gggcaacata atgttttaaa cgcgttggct gcgattgctg ttgcaacaga
     2341 agaagggatt tcggatgaag cgatacaacg cgggctttta aagtttgagg gtgtagggcg
     2401 gcggttccaa ttattgggag aaataacgca tcataataaa aaggctttaa tgatcgatga
     2461 ttatggtcat catccacgcg agatccaagt tgttttagaa gcgttacgtg caggttggaa
     2521 cgatagaagg ctcgtgatgg tttttcaacc gcatcgttat acccgaacca aagcgttatt
     2581 tgaagatttt gtttccattc tatcgctggt agatgtatta attttattgg atgtgtattc
     2641 tgctggcgaa gtagccattg cgggtgcaga tagtcgaacg ctggctttta gtattcgaag
     2701 tcgtgggcgt gttgatccta tttttattgc agacaagcgc gagctttcgg gggttttagc
     2761 cacggtatta gaagacaatg atgtgttgtt aatgcaaggc gcgggtgata ttagtgcgct
     2821 tgcaacggaa ttggctgtta atgcaaaaaa gtgagagagg gtaggtatga gaggactctt
     2881 aaaatttaac gaaccattag caatgcatac gtcttggcat attggtggcc ctgctgataa
     2941 atattttcgc ccggcagata cgaaagatct cgcagaattt ttaaaaacaa gcgtaaaaga
     3001 taatgagcct attacttggt taggattagg tagtaatgta ttaatttcgg atagtggcat
     3061 tcgcggtgtg gtgatccata ccttagcaca agcgggtgct ggcgctatta ctgtttctga
     3121 gcccgaaagc aatacagaga aagttttgat ttgggtagat gcgggcgtaa cgtgtgcaaa
     3181 attggcaaaa tattgtgcaa aagaagggct tttgggtggt gaattttttg cggggattcc
     3241 gggaaccctg ggtggtgctc ttgccatgaa tgcgggtgcc tggggcgggg aaacttggcg
     3301 tttggtgaaa caggttgaag tgattaatcg ctatggtgag cagacaatac gttcttcgaa
     3361 tatgtataaa attaagtatc gatctgtcga aaggatacgc gataattttc aaaataatgt
     3421 gcctgaaaaa ttacacgaag aatggtttat tgcgggatgc tttgagttcg agcgcggtga
     3481 ttcggctatt gcaacacgaa aagtaaaagc actcttgcaa gaacgtacag caaagcagcc
     3541 tattggtgtt tttagttgtg gatccgtctt taaaaatcca gaagggaatc atgcaggtaa
     3601 attaatagag gcttctcaat tgaaaggttt tacaattggc ggggcacaaa tttcaccaaa
     3661 acatgctaat tttattttaa acttgggcta tgcaaaagcc caggatgttt tggcgcttat
     3721 aaaacatatt caagccgttg ttttaaaaga tcatcaaata tgcctggaac ccgaagttcg
     3781 gatattagga ttttaaatta gctggaaagc agaaattgtt aatttgccaa ctaatcgtag
     3841 cttgcattgt attaagttgt catacatatt tattaatggc atagccagaa aaggtgtcta
     3901 cgaggcagaa atcaagtaca atgccccatt tagttgtgcg caaggaagct taaataaggc
     3961 agggaagatg taggagaggc gaataataaa tgtctgttag taggatgcct atggtatcca
     4021 tttttttacc actagcaata atactggtca gcttcttgtc gcttgtggta tggtcgcgac
     4081 attccgatcg gtttccattg agaacggtgg aaattcgctc gtccttgcat cgcgtccaag
     4141 agacagagat acaggcgagt gtattacctt ttttcgataa aggttttttt tggttaaatg
     4201 tacaagcggt gcaggcctgt ttaaagcgga taccttgggt tcaaaccgcg gatattagac
     4261 gtgtatggcc ggatcggctt ctggtttcgg tgcaagaaaa aacagcacag gcgaaatggg
     4321 gagaaaaagg ggtgttgagt
//
LOCUS       DAORVT010000064         4338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_15206_length_4338_cov_2.983189, whole genome shotgun sequence.
ACCESSION   DAORVT010000064 DAORVT010000000
VERSION     DAORVT010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15206_length_4338_cov_2.983189"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /gene="rplQ"
                     /locus_tag="PK389_01960"
     CDS             complement(<1..314)
                     /gene="rplQ"
                     /locus_tag="PK389_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HQY22508.1"
                     /translation="MRHRKAGRRFKRTSSHRKAMFQNMINSVIRYEMIKTTLPKAKDL
                     RRVLEPFVTLAKIDTIANRRLAFDRLRDKESVSKLFAEVGPRFKERKGGYIRILKCGN
                     RKG"
     gene            complement(333..1319)
                     /gene="rpoA"
                     /locus_tag="PK389_01965"
     CDS             complement(333..1319)
                     /gene="rpoA"
                     /locus_tag="PK389_01965"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HQY22509.1"
                     /translation="MQRAVTEFLKPRLVDVQQVGDQRAKVVVEPLPRGFGHTLGNALR
                     RVLLSSMPGCAVIEAYIEGVLHEYTTKEGVQEDIIDILLNLKGIEFTLEGREEVELKL
                     NKQGEGPVLASDFELPHDVKIINPNYVIAHMTKAGDLKMTIRVVRGRGYEPISMRQNP
                     EEETKSIGWLQLDASFSPVRRVTYVVENARVEKRTDLDRLVLDLETNGTIEPEEAIRW
                     AASILVDQLSVFVELKGEKQAEPLRLRTEIDPLLLRSVDDLELTVRSANCLKAENIFL
                     IGDLIQKSEVELLKTPNLGKKSLTEIKDILTNKGLSLGMQIENWPPLSLQQD"
     gene            complement(1333..1959)
                     /gene="rpsD"
                     /locus_tag="PK389_01970"
     CDS             complement(1333..1959)
                     /gene="rpsD"
                     /locus_tag="PK389_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018139892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HQY22510.1"
                     /translation="MARYLGPTCRVQRRLGTDLGLKSRVREIASKCKLATPPGMHGAK
                     KTRRTTDHGVQLCEKQKFRYMYGVLERQFRKYYTEAARRKGATGENLFQLLECRLDNV
                     VYRLGFGCTRAEARQLVRHKAILVNDKIVNIPSCAVKAGDVITVREKSKTQVRVQDSL
                     RLAEGAGFPEWVDVDVSIMKGTFKRIPDRSELPTEFNEQLVVELYAKK"
     gene            complement(1977..2411)
                     /gene="rpsK"
                     /locus_tag="PK389_01975"
     CDS             complement(1977..2411)
                     /gene="rpsK"
                     /locus_tag="PK389_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HQY22511.1"
                     /translation="MAKASTAKASSKASGSKPTAAVRTRKKVKRTVTTGTVHVFASFN
                     NTIITVSDSQGNTIAWQTAGAAGFKGSRKSTPYAAQMAARKAVLQAKEFGLKTVDVNV
                     RGPGPGRESAVRAVGENLMILRIIDKTGVPHNGCRPSKERRV"
     gene            complement(2428..2820)
                     /gene="rpsM"
                     /locus_tag="PK389_01980"
     CDS             complement(2428..2820)
                     /gene="rpsM"
                     /locus_tag="PK389_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010646232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HQY22512.1"
                     /translation="MAVRLSGIILPPNKHIRIALRSVYGIGSHRAMAICKKVGIEPSR
                     KVSDLKDEYTTVLQEAVNEYVVEGDLRRRVAMDIKRLKDIKCYRGRRHTLGLPVRGQR
                     TKTNARTRKGRKRQSQMAMKPQSTEAKK"
     gene            complement(2823..2939)
                     /gene="rpmJ"
                     /locus_tag="PK389_01985"
     CDS             complement(2823..2939)
                     /gene="rpmJ"
                     /locus_tag="PK389_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HQY22513.1"
                     /translation="MKVRASVKKMCDGCVVVRRERVVKIVCKKTSKHNQRQG"
     gene            complement(3067..4332)
                     /gene="secY"
                     /locus_tag="PK389_01990"
     CDS             complement(3067..4332)
                     /gene="secY"
                     /locus_tag="PK389_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006704131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HQY22514.1"
                     /translation="MDAIPPWSDEIAAVLLIVFGLVSFLSLLNVSDGNNTIAAAWSNA
                     LRGLFGYGSAIVCGGLFALGIMPYISASIIIQLLTVVWPTLEQLKKEGQVGRKKISQY
                     TRYSTLALATLQAVGMARYLANSGIALDPNLTYYFIVTLTLVSGTMFLMWLGEQITER
                     GVGNGISMIIFAGIVSGLPSAVIGTLEKVSQGEMQAIGLVLLCVMLMAIIAFVVFIER
                     GQRRITVNYAQRQQGRKMYAAQKSHLPLKLNMAGVIPPIFASSFILFPATIAQWFGKT
                     PGFEWLTALSLALSPGQPCYILLFAAAIIFFCFFYTALVFNPKETAENLKKSGGYIPG
                     IRPGDQTAQYIDKVMTRLTIVGAIYITLVSLLPEFLVMGWNVPFYFGGTSLLIIVVVV
                     MDFMAQVQAHLMSHQYESLMKKANLKGSR"
BASE COUNT         1233 a         1089 c          742 g         1274 t
ORIGIN      
        1 cctttacgat tgccacattt taaaatgcga atatatccac cttttcgttc tttaaaacgc
       61 ggaccgacct ctgcaaataa ttttgaaacg ctttctttat ctcgcaaacg atcaaatgct
      121 aaacggcgat tcgcaatagt atcgatctta gccaatgtca caaaaggctc aagtacacgc
      181 cttaaatctt tggctttcgg taaagttgtt ttaatcatct catagcgtat cacagaatta
      241 atcatattct ggaacatcgc ctttcgatgg ctggatgtgc gtttaaacct tctacctgct
      301 tttctatgac gcataaatat aacccttgtt gcttaatctt gttgtaaact taacggtggc
      361 caattttcta tttgcatgcc aagcgaaagg cctttgtttg ttaaaatgtc tttaatttca
      421 gttaaagatt ttttgcccaa attaggggtt ttcaacagct cgacttcgga tttttgaatg
      481 agatccccaa tcaaaaagat gttctccgct tttaaacaat tcgccgaacg aacggttagc
      541 tcaagatcat caaccgatcg taacaataac ggatcaattt ctgttctcaa acgaagcggc
      601 tctgcttgct tctcgccttt taattctaca aaaacggata attgatcaac caaaatactc
      661 gcagcccaac gaatcgcttc ttcgggctca atggtaccat tcgtttctaa atccaaaaca
      721 agtctgtcta aatcagttcg tttttccaca cgcgcatttt caaccacata ggtaacacgc
      781 cgaaccggac taaatgaagc atccagttgc aaccacccaa tggatttcgt ttcttcttct
      841 ggattctgac gcatgctgat gggctcatac ccccgccctc tcacaacacg aatagtcatt
      901 ttcaaatcgc ctgctttagt catgtgcgcg atcacgtaat taggattgat aatcttcaca
      961 tcatgcggca attcaaaatc agaagctaaa acaggacctt cgccttgttt atttaattta
     1021 agctcaactt cttctcgacc ttccaatgta aattcaatac ctttcagatt tagcaaaata
     1081 tcaataatat cttcttgtac accttctttt gtcgtatatt catgaagcac accctcaata
     1141 tatgcttcaa tgactgcaca tcccggcata gatgaaagca aaacacgcct taacgcattc
     1201 cctaaggtat gtccgaaacc acgcggaagt ggctctacca cgactttagc gcgctgatct
     1261 cctacttgtt ggacatcaac taatctgggc tttaaaaatt ctgttacggc acgttgcatg
     1321 aattgctcct cactacttct tcgcgtaaag ctctacgacc aactgttcat taaattcagt
     1381 aggtaattcg cttctatcag gaatacgttt gaatgtgcct ttcattatac tgacatccac
     1441 atccacccat tctggaaaac ctgccccttc agctagccga agggaatcct gaactcgtac
     1501 ttgtgtttta gacttttctc gtactgtaat gacatctccc gcttttaccg cacaagaagg
     1561 aatattaaca attttatcat tcaccaatat cgctttatga cgcactaatt gacgtgcttc
     1621 ggcacgcgta catccaaatc ctaagcggta aacaacgtta tctaaacgac attctaataa
     1681 ctgaaataga ttttcacccg tagcgccttt gcgtctagcg gcttctgtat agtatttacg
     1741 gaattgtcgc tccaacacac cgtacatgta tctgaatttt tgtttctcgc aaagctgtac
     1801 accatgatcg gtcgtacgtc gcgtcttttt tgctccatgc atacccggcg gtgtagccag
     1861 cttacactta gaagcaattt ctcgaacacg actcttcaaa cctaaatcgg ttcctaatct
     1921 tctttgtact ctacacgtag gccccaagta tcttgccatt tcattatcct caaatattaa
     1981 acacgacgct ctttcgatgg tctgcaccca ttatggggaa cacccgtttt atcaataatt
     2041 cgcaatatca ttaaattttc acctaccgcg cgaacagcag attctctgcc aggcccaggt
     2101 cctcgaacat tcacatccac cgtttttaaa ccaaattctt ttgcttgcaa aacagctttt
     2161 cttgctgcca tttgcgctgc atatggcgtg cttttacgag atcctttaaa cccagcagca
     2221 ccggcggttt gccatgcaat cgtattccct tgagaatccg atacggtgat aatggtatta
     2281 ttaaaagatg caaacacatg cacagtgcct gtcgtcactg tacgttttac tttcttacgc
     2341 gttctcactg cagctgtggg tttagagccc gaagctttcg atgatgcctt tgctgttgat
     2401 gcctttgcca tttacaaatt tcctacgcta tttttttgcc tctgttgatt gcggcttcat
     2461 agccatttga ctctgtcgct tacgtccttt tcgagtacgc gcatttgtct tagtacgttg
     2521 tcctcgaacc ggtaaaccca atgtatgacg acgaccccga taacatttaa tatcttttag
     2581 tcgcttaata tccatagcaa cccgacgacg cagatctcct tctacaacat actcattcac
     2641 tgcttcctga agcactgttg tatattcatc ttttaaatcg cttacctttc ttgaaggctc
     2701 gatacccact tttttacaaa tagccattgc tctatggcta ccaataccat aaacactgcg
     2761 caacgcaatt cgaatatgtt tattgggcgg aagtataatt cctgatagtc gtacggccat
     2821 aattaaccct gcctttgatt atgtttgctt gtcttcttgc agacaatctt gactacccgt
     2881 tcgcggcgga caacaacaca tccgtcgcac atttttttaa ctgatgctct tactttcatc
     2941 ctcgtcctca aatagccgtc gcataaatag atgacaattc tacgcctata aatatataaa
     3001 ggctagccct aaaggagtga acacataaac aattacttga gttatgtatc cgcccctact
     3061 gaaaaattat cgcgaacctt ttaggttggc tttcttcata agtgattcat attgatgaga
     3121 catcaggtgt gcttgcacct gagccataaa atccatcaca accactacaa taatcaacaa
     3181 agaagtacct ccaaaataaa aaggtacatt ccatcccatt actaaaaatt cgggcaataa
     3241 agaaaccaat gtaatataaa ttgctccaac aatcgttaaa cgcgtcataa ccttgtcaat
     3301 atactgcgct gtctgatcac caggtcgaat cccgggaata tacccacccg attttttcaa
     3361 gttctcggcg gtttcttttg ggttaaatac caatgccgta taaaaaaagc aaaagaagat
     3421 aatcgctgct gcaaataata aaatataaca gggctgtccg ggcgataatg caagactaag
     3481 tgctgttaac cactcaaaac ccggtgtttt tccaaaccat tgtgcaatag tcgctggaaa
     3541 caaaataaaa ctcgatgcaa atatcggcgg aataacgccc gccatattga gctttaaggg
     3601 caaatgactc ttttgtgccg catacatttt tcgaccttgt tgtcgttgcg cgtaattaac
     3661 cgtaatacga cgctgcccac gttcaataaa aacgacaaaa gcgataatgg ccattaacat
     3721 tacgcataac agcacaagac cgatagcttg catctcaccc tggctcacct tctctaaagt
     3781 accaataacc gcactgggta atcctgagac aatacccgca aaaataatca ttgaaatacc
     3841 attacctacc ccacgttcgg ttatttgctc tccgagccac attaagaaca tcgtgccgct
     3901 aacaagcgtt aaggtaacga taaaataata agtaagattc ggatccaagg ctattcccga
     3961 attggcaaga tatcgtgcca tgcccacagc ttgcagtgtt gctaaagcga gggttgaata
     4021 acgcgtatat tgcgaaatct ttttgcgtcc tacctgccct tctttcttta actgttccaa
     4081 ggttggccaa accactgtta acaactgaat aataatggag gcggaaatgt agggcataat
     4141 ccctaacgca aataaaccgc cgcatactat cgcgctgccg tatccaaata gcccacgcag
     4201 cgcatttgac cacgcggcag cgatggtgtt gttaccatct gaaacattca acagcgatag
     4261 aaatgatacc agcccgaaca cgatcagcag aactgcggcg atctcatcgc tccatggcgg
     4321 aatcgcatcc atcactga
//
LOCUS       DAORVT010000065         4302 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_15431_length_4302_cov_3.700494, whole genome shotgun sequence.
ACCESSION   DAORVT010000065 DAORVT010000000
VERSION     DAORVT010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15431_length_4302_cov_3.700494"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /gene="traT"
                     /locus_tag="PK389_01995"
     CDS             complement(<1..399)
                     /gene="traT"
                     /locus_tag="PK389_01995"
                     /inference="COORDINATES: protein motif:HMM:NF017619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HQY22515.1"
                     /translation="MLFLKARATFKAILLISALTLLLGCGATHTAINKRKLDVQTKMS
                     STVFLDPMTSDKKTVYLQIRNTSDKSELDLEHPVAEVMLAKGYTRVMAPEQAQILLQT
                     NILQVGRSDLRAADHALNQGFGAALGGAVAG"
     gene            587..847
                     /gene="rpmB"
                     /locus_tag="PK389_02000"
     CDS             587..847
                     /gene="rpmB"
                     /locus_tag="PK389_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003117157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HQY22516.1"
                     /translation="MSRVCQVTGKAVMAGNNVSHSNRKTRRRFLPNLQSHRFWIPGQV
                     PGKGRFIKLVVSHKGMRTIDKLGIEAVLAALSAREKKAVGDN"
     gene            860..1015
                     /gene="rpmG"
                     /locus_tag="PK389_02005"
     CDS             860..1015
                     /gene="rpmG"
                     /locus_tag="PK389_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020401238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HQY22517.1"
                     /translation="MARKSINVRLVSESGDFYTTSKNPKMAHKLKLRKFDKKTRKHEW
                     FEEKKIK"
     gene            1022..2026
                     /gene="birA"
                     /locus_tag="PK389_02010"
     CDS             1022..2026
                     /gene="birA"
                     /locus_tag="PK389_02010"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:NF008847.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HQY22518.1"
                     /translation="MTRTLKTLIQILSDGEFHSGERLGKQLSLTRSAIWKLIKQLACW
                     DLEIESVTNKGYCIPGGLTLLETAKISSFIKKSQKSQLEQIEIFDSLPSTNDYLLNVK
                     STNASPEAPKKNRACFAEKQTQGKSRRGRTWVSPFAKNIYLSLLWHFEKDPSELSGLS
                     LAIAIATVKTLHAFGIAKDFGIKWPNDVLWENHKLIGILIELSGEANDVCNAVIGVGL
                     NVNMPNSALYDRNISQPWTDLQRLSNQKIDRNKLAGILLNNLIATAALFQKQGLTAFL
                     PQWQKLDLTLNKPVTIITNTMELQGTGRGINEHGHFLLETKLGQIKHFASGEVSLRLS
                     "
     gene            2148..4184
                     /locus_tag="PK389_02015"
     CDS             2148..4184
                     /locus_tag="PK389_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HQY22519.1"
                     /translation="MLLTKLRTFLLGKTLNPTKPEARRSMLLVAFLAWVGLGADGLSS
                     ACYGPEMAFLALGQHPHLALYLAAAIALTVFVIALGYNQVIELFPSGGGGYKVATQLI
                     GPYAGLTSGVALIVDYVLTVCVSIASCVDALFSLFPLEAQSHKLITEIALLVLLGFLN
                     LRGIKESIKILIPIFLTFFITHTLLILYGILKHSERLTHLIPDTYHETVNLNHEAGWI
                     FILALFLRAYSLGGGTYTGIEAVSNNVNRLSPPRVTTGKWAMGYMAFSLSFIAGGIIL
                     LYLLWDVVPTAGLTLNAVIFKTIMSNWIWHDIDIGSFLFLLIMASEAGLLFVAANTGY
                     IGGPAVLSNMASDSWFPAQFRHLSSRLVTQNGIILIGITALLILLWTRGHLQLLIIMY
                     SINVFLTFSLSLCGLSRHWFKARKTEKNRILKLCLSLFGLMLTSSILIVTIFEKFEEG
                     GWVTLLVTGLLIFTCLFIKRHYTRFNAAMKKIDTQFKQTDIQKITPLPLDPHQPTAIF
                     FVGEHVGIGMYSLKKVLKLFPNQYKNFVFLSVGEFNGQTYDPEANQQDPEADIKEMRT
                     KVVRRLSYFVNYCHIRNLPAVDYQTSDVDVIDGLTRLAKLKSRRYPNCIFFAGTLIFE
                     RENWINHSLHNHTAKLVQERLQSEGMYMMLLPIRMADILKEEAEEAIYTRPHFK"
BASE COUNT         1286 a          917 c          833 g         1266 t
ORIGIN      
        1 ccccgcaaca gcgcccccta gtgcagcgcc aaaaccttgg ttcaaggcat gatctgcagc
       61 tcggagatcc gaacgcccaa cctgtaagat attggtttgc agtaaaatct gtgcttgttc
      121 gggtgccata acacgcgtat agcctttagc taacattact tccgccactg gatgttctaa
      181 atctaactca gatttatccg aggtatttcg aatttgtaaa tagacagttt ttttatctga
      241 agtcataggg tctaaaaaca cggtcgaact catttttgtt tgaacatcga gttttcgctt
      301 attgattgcc gtgtgtgtgg cgccacatcc gagcaaaagt gtaagcgcac ttattaaaag
      361 gatcgcctta aatgtcgctc tcgccttcaa aaacaacatt aatcttcctc cttacttggc
      421 atcaacaacc gttagagaca actgtaagtt attagctcgg agtatacctg gctcatacta
      481 aggtggaaag aggcgctgat actgcttgat ctttgtgcct tgccttggta taaatgcgct
      541 aagattatcg attaatcaat attgtatagc attttgggga cttatcatgt ctagagtttg
      601 tcaagtaact ggaaaagccg ttatggctgg aaacaacgtc tcgcattcta accgaaaaac
      661 gcgcagacgg tttttgccta atcttcaaag ccatcggttt tggatcccag gacaagttcc
      721 aggaaaaggt cgctttatta aattggttgt ctctcataag gggatgcgca ccattgataa
      781 gctcggcatc gaggctgttt tagccgcctt atctgctcgc gaaaaaaaag cagtaggcga
      841 taattaggag ttccccaaca tggcaagaaa atctattaat gtacgactcg tctctgaatc
      901 tggcgatttt tataccacca gcaaaaatcc aaagatggcg cataaattaa aattacgtaa
      961 atttgataaa aaaacacgta aacatgaatg gtttgaagaa aagaaaatta agtaatttgt
     1021 cttgacccga accctcaaaa cgctaattca aatactgagt gacggcgaat tccattctgg
     1081 agaacgcttg ggtaaacagc tctcgttgac gcgtagtgct atctggaaac tcattaaaca
     1141 gcttgcgtgt tgggatcttg aaatagaatc cgtcaccaat aaaggctatt gcataccagg
     1201 cggtctcacc ttactcgaaa cagcgaaaat ttcatccttt ataaaaaaat cacaaaaaag
     1261 ccaactggaa caaattgaga tattcgacag cctaccctct acgaatgatt atttattaaa
     1321 tgtaaaatca acaaatgcga gccccgaagc acctaaaaaa aatcgcgcgt gctttgcaga
     1381 aaaacaaacg caggggaaaa gccgacgtgg acgcacatgg gtatcccctt ttgctaaaaa
     1441 tatctactta tctctactgt ggcattttga aaaagatcct agcgaattat ctgggttaag
     1501 tcttgctatc gcaatagcaa cggttaaaac attgcatgca tttggtattg ctaaagattt
     1561 tggaatcaaa tggcccaatg atgtgttatg ggaaaatcat aaacttatcg gcatcttgat
     1621 cgaactttct ggtgaagcca acgatgtctg caatgccgtc atcggcgttg gcttgaatgt
     1681 caacatgccg aattcagccc tttatgatcg caatatctcg caaccctgga cagatctaca
     1741 aagactctca aatcaaaaaa ttgatcgcaa taaattagcg ggcatactgt tgaataatct
     1801 tattgcaacc gctgctctct tccaaaaaca aggactgaca gcctttttgc cgcaatggca
     1861 aaaattagat ttgaccttga ataaacctgt gaccattatt accaatacga tggaattaca
     1921 aggaacaggg cgcgggatca atgaacatgg tcacttcctg ctcgaaacaa agctcggaca
     1981 aattaagcac tttgccagtg gagaagtctc gcttcgactg agctaaatgc tctgaattta
     2041 ctgacaaatg aagcatacac gaccttttac aaaaacaaaa ataagctact attaaataat
     2101 gccataatcc tacagaaaat ttttgaaaag ggagtgcttg ttttagcatg ttactcacaa
     2161 aactccgaac atttttgctc ggaaaaacac tgaaccccac aaagccagaa gcgcgccgta
     2221 gtatgttgct ggtggctttt ctggcatggg taggtctagg cgcagatggt ctttcttcgg
     2281 cgtgctatgg acctgagatg gcctttttag cgctaggaca acacccgcac ttagcgctat
     2341 atcttgctgc tgccatcgcc ttaaccgttt ttgtgattgc attgggttac aaccaagtca
     2401 ttgaactctt tccctcgggc ggcggcggct ataaagtcgc gacacagtta attggtccgt
     2461 atgcgggcct cacctctggt gttgccttga ttgtagatta tgtattaacc gtctgtgttt
     2521 cgatcgcaag ctgtgtggat gcactcttta gtttgtttcc tctagaggca caatctcata
     2581 aactaataac cgaaattgct ttattagtac tattaggatt ccttaatctt cgtggtatta
     2641 aagaatctat taaaatactg atccccattt ttttgacttt ttttataacc cacacgcttc
     2701 tcattcttta cggaatactg aaacattcag aacgcttaac acatttaatt ccagacacct
     2761 atcacgaaac tgtcaacctt aaccatgagg caggctggat attcatttta gcactgtttt
     2821 tacgtgctta ttcgctcggt ggcggaactt atactggtat tgaagcggta tctaacaacg
     2881 ttaatcgcct atcaccacca cgggttacca ccggaaaatg ggccatgggt tatatggcat
     2941 tttcgctttc ttttattgcc ggtggcatta ttcttttata tttattatgg gatgttgtac
     3001 caacagcagg tcttacttta aatgctgtta tatttaaaac cattatgagt aattggatat
     3061 ggcatgacat agatattggg tcatttctat ttcttttaat catggcatct gaagctggac
     3121 tgttatttgt cgctgctaac accggctaca ttggaggacc tgcagttctt tcaaatatgg
     3181 cttcagattc ttggttccct gcgcaatttc gtcatctatc ctctcgatta gtgacacaaa
     3241 acggcattat cctgattggc ataaccgcct tattgatctt gctgtggaca aggggacact
     3301 tacaactttt aattattatg tatagcatca atgtgttttt aacttttagt ctctctttat
     3361 gtggactttc acgccattgg tttaaagcca gaaaaacaga aaaaaatcga atattaaagc
     3421 tctgcctttc gttatttggt cttatgctca cttccagcat tttaatagtg accatttttg
     3481 aaaagtttga agaaggcgga tgggtaaccc tattagtaac cggacttttg atttttacat
     3541 gtctctttat taagcgacac tatacacgat ttaatgctgc aatgaaaaaa atagataccc
     3601 aatttaagca aaccgatatt caaaaaatta caccgctccc acttgaccca caccaaccca
     3661 ccgccatttt ttttgtggga gagcatgttg gcattggcat gtatagcctt aaaaaagtac
     3721 taaaattatt tccaaatcaa tataaaaatt ttgttttctt aagcgtcggc gaattcaatg
     3781 gacaaaccta tgatcccgaa gccaatcaac aagatccaga agcggatatc aaagaaatgc
     3841 gcacaaaagt tgttcgtcgc ttaagttatt ttgtcaatta ttgccatatt cgaaatttac
     3901 ctgcggttga ttatcaaacc agcgatgttg acgtaataga cggcttaact cgactcgcta
     3961 aactaaaaag tcgacgatat ccaaactgta ttttctttgc cggcactttg atttttgagc
     4021 gcgagaattg gattaaccat tcgttacata atcatactgc aaaactcgta caagaacgat
     4081 tacagtctga aggcatgtat atgatgcttc tacctattcg catggcagat atcctaaagg
     4141 aagaagcaga ggaggcaatt tatacacgcc cccattttaa ataacgcaca gccaaagcaa
     4201 gcaccacaat tttaacgcaa cctggaataa taaaagggat taatccacct ttaatgtgct
     4261 gcgagcctcc agcatagtca ctgcaggcaa cgttttgtct tc
//
LOCUS       DAORVT010000066         4230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_15876_length_4230_cov_3.030898, whole genome shotgun sequence.
ACCESSION   DAORVT010000066 DAORVT010000000
VERSION     DAORVT010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15876_length_4230_cov_3.030898"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PK389_02020"
     CDS             <1..320
                     /locus_tag="PK389_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22520.1"
                     /translation="LDACLVEKSFILQHVSEPDRLIMASTEATGVSLIVIFMSGFLFS
                     FFSSLSFWVSTLISIFLSILSQIYMIYKTHKDPYFFNVWMAALSCKKTKNYFKLSDNH
                     YGD"
     gene            343..2709
                     /locus_tag="PK389_02025"
     CDS             343..2709
                     /locus_tag="PK389_02025"
                     /inference="COORDINATES: protein motif:HMM:NF015118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22521.1"
                     /translation="MTSLVMLAFAIVIGMVFSVTLRTFFFPEPKVANLGDVLPLVAII
                     QDKVILGKRGSCSAIIKINGIDSGSKSAGEIEALLTTKQHWLDKMAEQGANFKIITLR
                     REYKHKVDSGDVPDLLRDIHEKWMENFEKTYSNTHYIVLTVYPNQRKDVFSFFKKELP
                     EVNTGLLKEQVALTLDGLNSYKPEVMHNDGEWSEIMAFLNELATAKKQSIRPKKYDIN
                     DALSHRMTFLKNSEIIKYDETQFAKIISLSKWDKVVSSEMLKEIQALSGKLEILQIFK
                     GYGKIKSLMKLNYQRKQKQLPFQNTYIEDEYRLAVEEVEAGRSSLYDYQLSVIVFAED
                     LKQLEALESQVKRIMMGYGITPATERLAKEEVYKSQFPGMDKMIRPTHPLSCNLSYLF
                     NFDSEPSGLEKCDWGEGPLRPFKTVSGSAYSLQFHVVEDKESVGHSLVVAPTRGGKTT
                     LFQHLIGGALRHPNLRAFIFDRLNGTRIFTESIGGNYVDLSDNEIPLNPFVCKDSIPN
                     RQFLKNFLLMLAKCNDDESANDAALAVEQILTVPVEERILKHCYANIAKKGSLFAMGL
                     RKWVLDDTLSPWFNGSKIGQNGKISAFDALDLSSNRLTAFEMTRIQQTPETAAAVTTY
                     LMHRIREIGRGAFPHLIFIDETAPMLEDATFSKSVDVLLREHAKLRGAVSLCFQDTTA
                     INSVILNQCQTWFLFPNHAANKEQYKMFDLTDFEWDYIKGFNRISKELKRSVLLKKPQ
                     ESVILNIDLSSLGNLLQLYRSGSEPVKIVRELQKQWGVNQWVDKYLSL"
     gene            2792..3490
                     /locus_tag="PK389_02030"
     CDS             2792..3490
                     /locus_tag="PK389_02030"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HQY22522.1"
                     /translation="MSHLKGFILLAALFVTSSAFAEDGMYVSLGAGLGKVSKVKSNQD
                     FPLVLKSPGVFATPITEMTLDFKNAIRINGAIGYKTGDFRVEFEPSYLKAKYKKIDQK
                     PYPLIAAKDLSGYVRSLSGFMNGYYDFPVSDTLVPYVGAGLGYTRIKNHLEHAGLVDF
                     NGGGQAVNLPTLKFNMSDNSLAYQAIAGFMVNLTNQFALTVDYRFSSTTKKMLAFNDK
                     LKSHVATLGLMYKF"
     gene            3589..>4230
                     /locus_tag="PK389_02035"
     CDS             3589..>4230
                     /locus_tag="PK389_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22523.1"
                     /translation="MKKMYQMLLMVVLFPAAQPGRCGGTPMHDPLSYARAFEILKAAK
                     TQIEETKRVQEELAGTKRLIGNFKEEAQNLRGELLNWRTYYDKIDTLDANEFSAMSWL
                     GLDRTIPARNAPEAFRLIDDKLFKTNSPEIKREDYTDTMNTLKYEREKLARKSIVTGI
                     VVAEKSKSNLNESKKKIIMATDKSIGASDLISVMKTQNEILGVIASELVQSREL"
BASE COUNT         1366 a          718 c          883 g         1263 t
ORIGIN      
        1 gcttggatgc ttgcctagtg gaaaaatcat ttattttaca acatgtttca gagccagata
       61 ggttgatcat ggcaagcaca gaggcgactg gtgtttctct tattgttatt tttatgagtg
      121 gtttcctctt cagttttttt tcaagtcttt ctttttgggt aagcaccctt atttcaattt
      181 ttttatctat cctgtcacag atctatatga tttataaaac gcataaagat ccgtatttct
      241 tcaatgtctg gatggcggcg ttatcttgta aaaaaactaa aaattatttt aagctaagtg
      301 ataatcatta tggagattag tttattatgt ataaaaatct tcatgacaag tttggtcatg
      361 ttagcttttg ctattgtgat tggtatggtt tttagcgtga ctttaagaac tttctttttt
      421 ccggaaccta aagttgcaaa cttgggcgat gttctgccgc tagtcgctat cattcaagac
      481 aaggttattt tgggaaagcg ggggtcctgt tcagcaatca ttaagattaa cggcattgac
      541 tccggctcta aatcggctgg agaaatagaa gccttgttga caaccaaaca acattggctt
      601 gataaaatgg ctgaacaagg cgctaatttt aaaattatta ctttaaggcg agagtataag
      661 cacaaagtgg atagcgggga tgttcctgat ttattgaggg atattcatga gaaatggatg
      721 gaaaattttg aaaagaccta ttcaaacact cattatattg tgctaaccgt ttacccaaat
      781 cagcgaaaag atgtttttag tttctttaaa aaagaattgc cagaagttaa tactggactt
      841 ttaaaagaac aagtcgcctt aactttagac ggattgaata gttacaagcc ggaagttatg
      901 cataacgatg gcgaatggag cgaaattatg gctttcttaa atgagttagc tacggcaaaa
      961 aaacaaagta ttagacctaa gaaatatgat attaatgacg cgctcagtca tagaatgaca
     1021 tttcttaaga atagcgaaat aattaaatat gatgaaactc aatttgccaa aattatctca
     1081 ttatctaagt gggataaggt tgtaagctca gaaatgttaa aggaaataca agccctatcc
     1141 ggcaagcttg aaatcctcca aatatttaaa ggttatggga aaattaaatc gttaatgaaa
     1201 ttaaattacc aaagaaaaca aaaacaactg cctttccaaa atacctatat tgaagacgaa
     1261 tatagattag cagttgaaga agtggaggcc ggtagaagca gcctatatga ttatcagcta
     1321 tcagtcatcg tatttgcaga agacttaaaa caattagaag cacttgagag ccaggttaaa
     1381 cgaatcatga tgggatatgg gattacccca gcgaccgaaa gactggccaa agaagaagtc
     1441 tataaatcac agtttccggg catggacaaa atgatacgcc caacccaccc gttgtcttgt
     1501 aacctttctt acctttttaa ttttgatagt gagccaagcg gtttggaaaa atgcgattgg
     1561 ggtgaggggc ctttaagacc ctttaaaaca gtatcgggta gcgcttattc actacagttt
     1621 catgttgtag aagacaaaga gtcggtaggt catagtttag tcgtcgcacc cacccgaggc
     1681 ggaaaaacaa cgctatttca acatttgatt ggtggggcat taagacatcc gaatttaaga
     1741 gcctttattt tcgataggct aaatggaaca cgtattttta ctgagtctat tggcggaaat
     1801 tatgtcgatt taagcgataa tgagatccca ctcaatcctt ttgtttgtaa agactccata
     1861 cctaatcgcc agttcctaaa aaacttttta ttaatgttgg caaagtgtaa cgacgatgaa
     1921 agtgctaatg atgcagccct tgcagttgaa caaattttaa cagtgcccgt cgaggaaagg
     1981 atcttaaaac attgctatgc taatatcgcc aaaaagggtt ctctatttgc catgggtttg
     2041 agaaaatggg tactggatga cactttaagc ccttggttca atggctctaa aataggtcaa
     2101 aacggcaaga tttccgcttt tgatgccttg gatttaagct caaatcgttt aaccgccttt
     2161 gaaatgacac gcatacaaca aactccagaa accgcagcgg cagtaacgac ttacctcatg
     2221 catcgtattc gggaaattgg caggggtgca tttccacacc ttatttttat tgatgaaacg
     2281 gcacccatgt tagaagatgc aaccttttca aaaagcgttg atgtgttact tcgagaacat
     2341 gcaaaactga gaggtgcggt ttctctttgt ttccaagaca ctacggcaat caattcagtc
     2401 attttgaacc aatgtcaaac atggtttttg ttccctaatc atgctgccaa taaagaacaa
     2461 tataaaatgt tcgatttgac agactttgaa tgggattata ttaagggatt taatcgcatc
     2521 tctaaagaat taaagcggtc ggtattactt aaaaagcctc aagagtccgt cattctaaat
     2581 attgatttat cctctcttgg taatttatta caactgtatc gttcaggctc tgagcctgtg
     2641 aaaattgttc gagaacttca gaaacaatgg ggggtaaatc agtgggtgga caaatattta
     2701 agcctttgaa atattatgat tgtatttgac caagcaagca ttttagaatg attacgatta
     2761 gttaattttt ttatcataag ggagaaatgt tatgagtcat ttgaaaggtt tcattttatt
     2821 agcagccctg tttgtcacga gtagcgcatt tgccgaagat ggcatgtacg tatcgctggg
     2881 tgcaggtctc gggaaggtaa gcaaagtaaa atctaatcaa gattttccgt tagttttaaa
     2941 atcacccggt gtttttgcga cacccattac agaaatgact cttgatttta aaaatgccat
     3001 aaggattaat ggagccattg gttataaaac aggagatttt agagtagaat ttgagcctag
     3061 ttacctcaag gctaaataca aaaaaattga ccaaaaacct tatccgctga tagctgctaa
     3121 agatctctca gggtatgtgc gctctctatc aggctttatg aatggatatt atgactttcc
     3181 cgtgagtgac accctggttc cgtatgtagg agcgggattg ggatacaccc gtataaaaaa
     3241 tcatttagaa catgcgggct tagtagattt taatggaggt ggtcaagcag ttaatttgcc
     3301 cactttgaaa tttaacatgt ctgataattc tttggcatat caagcgatcg caggttttat
     3361 ggttaattta accaaccagt ttgctttaac agtagattat cgtttttcat ctacgacaaa
     3421 gaaaatgctc gcatttaatg acaaactaaa aagtcatgtt gcaacattag gattgatgta
     3481 taaattttaa tgtgattggg ctttgatgga atatgcgtta gcccttaacg aatggcatca
     3541 tttatttcat caaagctttt tgattatgta aaggagtgga cccctaaaat gaaaaaaatg
     3601 tatcaaatgc tcctgatggt ggttttgttt cctgccgccc aacctgggag gtgtggtggg
     3661 acgccaatgc acgatccttt aagttacgcg cgggcgttcg aaatcctaaa agccgcaaag
     3721 acacagatag aggaaaccaa aagagttcaa gaagagttag ctggaactaa gcgacttatt
     3781 ggcaatttca aagaagaggc tcagaattta agaggcgaat tgctgaattg gcgcacctat
     3841 tatgacaaaa tagacacctt ggacgctaat gaattttctg ccatgagttg gttggggttg
     3901 gataggacga ttcctgctag aaacgcacca gaggcattta gactcattga tgataagtta
     3961 tttaaaacaa acagtcctga aattaaacgc gaagattata ccgacaccat gaatacgttg
     4021 aaatatgaac gcgagaaatt agccagaaaa tcgattgtga cgggtattgt ggtagctgag
     4081 aaaagcaaaa gcaatctgaa tgaatcaaag aaaaaaatca tcatggctac agacaaatct
     4141 ataggcgctt ccgatttaat aagcgttatg aaaacacaga atgaaatcct gggtgtcatt
     4201 gcaagcgaat tagttcaaag tcgggaatta
//
LOCUS       DAORVT010000067         4218 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_15969_length_4218_cov_3.975979, whole genome shotgun sequence.
ACCESSION   DAORVT010000067 DAORVT010000000
VERSION     DAORVT010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15969_length_4218_cov_3.975979"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="PK389_02040"
     CDS             complement(<1..831)
                     /locus_tag="PK389_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HQY22524.1"
                     /translation="MKLAIVYTRASLGIQAPLVTVEAHISGGLPAFTIVGLPETAVKE
                     SKDRVRSAILNSQFDFPSRRITINLGPADLPKEGGRFDLPIALGILAASQQIPSDCLN
                     EYEFAGELALSGELRSIQGALPVALGARKAERILVLPIMNATEAALPGDNTIYAANTL
                     LEITEHLCRRKQLHPFKQALPSSTDSDRTEYDLCDVRGQMHAKRALEIAAAGGHSLLM
                     IGPPGTGKTMLASRLPGILPSLTLEETLEVIAIHSLIPNSEPALQWGRRPFRAPHHTA
                     S"
     gene            complement(958..2943)
                     /locus_tag="PK389_02045"
     CDS             complement(958..2943)
                     /locus_tag="PK389_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005002052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HQY22525.1"
                     /translation="MEQFQYILILLGCAVSVVAVFRRIHLPPILGYLFVGMLVGPGGF
                     AFIPSIQDLSFLAEFGVVFLMFTIGLEFSFARLLTMRRALLGLGGIQVLLCTMVAMLG
                     GWYFGLAPKTAFVAAGALALSSTAVVIKQLAEQHELHSVHGRLSISILLFQDLAAVLF
                     LILIPALASKQETALWIPLCFALVKGVGVCVVLSFLGQRLLRPLFHEVAKSHSNELFM
                     MAALMVVLGAAWLTHHLQLSMALGAFLAGLMLGETEFRHQIEIDIRPFRDVLLGLFFI
                     TIGALLRIDTLPANGLWVLIILASLIVFKTLLIMGLARFVGAVSLGKAFRTGLILAQG
                     GEFGFVILTVAINNKLIDPIQSQVVVAAVVLSIVAAPVLIRYNKAIGQFLFKQDTVPP
                     PEEIKDVQLSEHTAELKDHVIICGFGRVGQILARFLEQENIPSVALDLDPMRISSSAL
                     AGEHAFFGDARRLEVLAAAGLARARMVVISFADEPAALETLRHIRSLRLDVPVFVRTR
                     NDSNLKTFQEAGATEVVPESLEASLMLASHLLLMLGVPASRILFKIRAIHSDRYRVIQ
                     GFFKGTDDPTLLEEADSARMSLHAITVNENAYAIGKTLREISDPESLLAIKVLTRGTT
                     RYPEPDPEIVIQAGDVLVLFATPEALYLLGEKILRGI"
     gene            complement(3001..3207)
                     /locus_tag="PK389_02050"
     CDS             complement(3001..3207)
                     /locus_tag="PK389_02050"
                     /inference="COORDINATES: protein motif:HMM:NF016280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HQY22526.1"
                     /translation="MTLKYLNLIKDELGDGFRRGFQVALQKCDLVTREEFDIQTEVLA
                     KTREKLEKLTRQITMLEENLKNTR"
     gene            3295..3861
                     /locus_tag="PK389_02055"
     CDS             3295..3861
                     /locus_tag="PK389_02055"
                     /inference="COORDINATES: protein motif:HMM:NF020972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQY22527.1"
                     /translation="MINRIKYALAMAFRHRKALSAYRRDKTLQGQSILTLDLMLFRTT
                     GIKIIALDFDGVLASHGATEIDLEIRNWLNRCIHIFGAGHVFILTNKPSRERSDYFSK
                     HFSGVEFIFPKRKKPYPDSILSILQKTGVSPSELLVVDDRLLTGILAAIIAGVRGYYM
                     TKPLVDLHNRRLSELFIMSLRKLERWVF"
     gene            complement(3913..>4218)
                     /locus_tag="PK389_02060"
     CDS             complement(3913..>4218)
                     /locus_tag="PK389_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HQY22528.1"
                     /translation="SALLVVWTIVNIVAWFEHWDPYPFILLNLFLALQAAYTGPVIMM
                     TQNRQAARDRLEAHNDYLVNIKAEQEIRTLLKHLNAQSQAIEELKGMVKSLQKNDLR"
BASE COUNT         1246 a          911 c          924 g         1137 t
ORIGIN      
        1 agaagcggta tgatgtggtg cgcgaaaagg tcttctgccc cactgtaaag caggttcgct
       61 gtttgggatt aatgaatgta tcgcgatgac ttctaaggtt tcttcgagtg ttaatgaagg
      121 caaaataccc ggaagcctac tcgccagcat agttttgccg gttccaggtg gtccaatcat
      181 cagtaaactg tgcccacccg ccgctgcgat ttctaaggcg cgcttagcat gcatttggcc
      241 gcgaacatcg cataaatcat attcggtacg atcgctatct gtgctggagg gcagcgcttg
      301 tttaaagggg tgcaactgtt ttcgtcgaca gagatgttcg gtaatttcta ataaagtgtt
      361 ggcagcataa atcgtgttat ctcccggtag cgccgcctcg gtcgcgttca tgatcggcaa
      421 aaccaaaata cgctcggctt ttcgagcacc cagtgcaacg ggtaatgcgc cttgaataga
      481 acggagttct ccggacaatg ccagctctcc tgcaaattca tattcattta aacagtcgct
      541 tggaatttgt tgagaggccg ctaaaatacc taaagcaatg ggcagatcaa agcgtccacc
      601 ttctttgggt aaatcagctg gacctaaatt aatcgtaatg cgacgcgagg ggaagtcaaa
      661 ttgactattt aaaatagcag aacgcacgcg gtctttgctt tctttcacag cggtttcggg
      721 caaacctaca atcgtaaaag cgggtagtcc acctgaaata tgagcctcta ccgttacaag
      781 cggggcttgt ataccaagag aagcgcgtgt ataaacaatt gctaatttca taccagttgt
      841 tatacgcgaa attaataggg ttacaatccg ctaaatagcg gatgtcgcgc tttttgaaga
      901 gcaggattgc ctgaagaaaa aaataaagat ttcgaactat gacatatatt tgaggagcta
      961 gatcccgcgg agaattttct ctcccaataa atagagcgct tctggcgtag caaacagaac
     1021 gagtacatcg cctgcttgaa tcactatttc gggatcaggt tccggataac gtgtagtgcc
     1081 acgcgttaac actttgatcg ccaagaggga ttcgggatcg ctaatttcac gaagggtttt
     1141 tccaatcgca tatgcatttt cattcaccgt gatggcgtgc aagctcatac gcgcactgtc
     1201 tgcttcttcg agtaaagtcg ggtcatccgt ccctttgaaa aatccctgaa taacgcgata
     1261 gcgatcagaa tgaatggcac gaattttaaa taaaatacgc gatgcgggaa cacccagcat
     1321 taagagtaaa tgcgaagcca gcattaaact cgcttctaac gattcgggca cgacttctgt
     1381 tgcaccagct tcttggaagg tttttaaatt agaatcgttt cgcgttcgaa caaaaacagg
     1441 cacgtccaac cgaagcgaac gaatatgcct gagggtttct agtgcagcgg gctcgtctgc
     1501 aaaactgatc accaccatgc gtgctcgagc gagcccggca gcagccaata cttcgagtcg
     1561 tctggcatca ccaaaaaaag cgtgctctcc ggctaaagca gaagaagaaa ttcgcatggg
     1621 gtccaaatct aatgcaaccg atggaatatt ttcttgctct aagaaacggg ctaaaatttg
     1681 cccaacacga ccaaatccac aaataatcac gtgatctttt agttctgccg tatgctcaga
     1741 cagctgtaca tctttaattt cttcaggtgg aggaaccgtg tcttgtttaa acaagaactg
     1801 cccaatggct ttgttgtatc gaatcaaaac cggcgcggca acgatactca atacaaccgc
     1861 ggcaaccacc acctgacttt gtatgggatc aatcaattta ttgttaatgg caaccgttag
     1921 aataacaaat ccaaattcac caccttgcgc taatattaag ccggtacgaa aggcttttcc
     1981 caaagaaaca gcgccaacaa aacgagccaa gcccataatt aataatgttt taaagacgat
     2041 aaggcttgcc aaaataatga gaacccaaag tccgtttgcc gggagtgtat caattcgcag
     2101 taaagcgcca atagtaataa agaaaagccc taacaataca tctctaaacg gccggatatc
     2161 aatttcgatt tggtggcgaa actcggtttc acctagcatt aaacccgcca agaatgcacc
     2221 aagcgccatg gataattgta gatggtgtgt tagccaggca gcgccaagga caaccatgag
     2281 tgctgccatc ataaataatt cgtttgagtg ggattttgca acctcgtgga ataagggtct
     2341 taataatcgt tggccgagaa aactcaagac tacgcaaaca ccaacaccct ttactaatgc
     2401 aaaacacagt ggaatccaga gtgctgtttc ttgtttgctt gctaaagcgg ggatcagaat
     2461 caaaaataaa acggcagcta aatcttggaa tagcaagatg ctgatagata gtctgccatg
     2521 taccgagtgt aattcgtgtt gctcggctaa ttgtttaatg acaacagccg ttgaagagag
     2581 cgctaatgcg cctgcagcaa caaacgctgt tttgggcgct agtccaaagt accatcctcc
     2641 taacattgca accatcgtac ataaaagaac ttgaatacca cccagcccta ataaagcccg
     2701 tcgcatcgtc aaaagtctgg cgaacgaaaa ttcaagacca atggtaaaca tcaagaagac
     2761 aacgccaaat tcggcgagga aactgaggtc ttggatgctg ggaataaacg cgaagcctcc
     2821 tgggccaacc agcatgccga caaaaagata accgagaatg ggcggaaggt gaatacgacg
     2881 aaacaccgca acaacactca ctgcgcatcc cagtaaaatt aaaatatatt gaaattgttc
     2941 catggactta gtttatcgca tttaagcttt tttcgaaagg cgctgggatt ctttatttaa
     3001 ttatcgagtg tttttaagat tttcttcaag cattgtaatt tgtcgtgtca gtttttcaag
     3061 tttctcacgg gttttagcta agacttccgt ttggatatca aattcttcgc gtgtgacaag
     3121 atcgcatttt tgcagcgcca cttgaaaacc tcgacggaat ccgtcaccca actcgtcttt
     3181 gataagattt aaatacttca gtgtcatgtt ggtattgtat gaggtctaat cccattttta
     3241 tacctgatta taaaaacagc ttcttttggt gctaaaaacg ctctatatta agttatgata
     3301 aaccggatta agtatgcctt agcgatggcc tttagacatc gaaaagcgtt gagcgcttac
     3361 aggcgcgaca aaacacttca gggtcagtca attttaacct tagatctcat gttgtttcga
     3421 actacgggca taaaaataat cgcactcgat tttgatgggg tcttagcatc acacggtgcc
     3481 acagaaattg acttagaaat tagaaattgg ctgaatcgtt gtattcacat ttttggtgca
     3541 gggcacgtct ttattttaac aaacaaaccc agtagagaac gctcagacta tttttcgaag
     3601 cattttagtg gtgtggaatt tatttttccc aaacgaaaaa agccctatcc cgatagtatt
     3661 ctgtctattt tacaaaaaac aggggtgagt ccaagcgaat tgttggtcgt ggacgatcgt
     3721 ttattaaccg gcatcttagc cgcgattatc gcgggcgtac gtgggtatta tatgactaag
     3781 ccattggtcg atttacataa ccgacggcta tcagaattgt ttataatgag cttacgtaaa
     3841 ttagagcgtt gggtattcta atgaatatcg aattccaata tgcttctatg aaggcggttt
     3901 ttgaacacga ttttatctaa gatcattttt ttgtaaagat tttaccattc ctttgagttc
     3961 ttcaatcgct tgactctgtg catttagatg ttttagcagg gtgcgaattt cttgttcggc
     4021 tttgatattg actaaatagt cattatgggc ttccaatcga tcgcgtgcag cttgtcgatt
     4081 ttgagtcatc ataatcacgg ggcctgtata agcagcctgt aatgctaaaa aaagattgag
     4141 cagtatgaag gggtatggat cccagtgttc aaaccaagcg actatattta caattgtcca
     4201 cacaaccaat aaagcgga
//
LOCUS       DAORVT010000068         4217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_15977_length_4217_cov_3.572561, whole genome shotgun sequence.
ACCESSION   DAORVT010000068 DAORVT010000000
VERSION     DAORVT010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15977_length_4217_cov_3.572561"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PK389_02065"
     CDS             complement(<1..313)
                     /locus_tag="PK389_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016205646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQY22529.1"
                     /translation="MISLNGIEKVIKKGNRVVHTLKGITLNIEQGEIFGILGHAGAGK
                     SALIRTINFLDPPSRGSVVIDSCDLSTLSATGIREARRQIGMIFEHFNLFNTRTVFEN
                     IA"
     gene            complement(399..1154)
                     /locus_tag="PK389_02070"
     CDS             complement(399..1154)
                     /locus_tag="PK389_02070"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HQY22530.1"
                     /translation="MSNLFFNLPSIQRGIIGHRGMSAQAPENTLAGFKKAAQFGLSWV
                     EFDTQRCASEEWVVFHDEKLDRTTNGTGLVIDTPYQTLKTLDAGSWFHPQFKNERVPT
                     LQDTLSCLADLKLHPNIELKVFEQPLSIEAIADFLNVLHNAWPKDLPPPLVSSFDLKS
                     LKILRSLDNKLPLGYIVRNPTENTLDKILEAGFDSLHCDNQNFSPALLDKATSKSIAL
                     PVLVYTVNDPNRMKTLLQKGVTAVFSDVTDVII"
     gene            1285..2574
                     /locus_tag="PK389_02075"
     CDS             1285..2574
                     /locus_tag="PK389_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HQY22531.1"
                     /translation="MSLTAVSSYLNYRFVKLPKTIGLTIITLVLSFFIAVSGKFGWDV
                     EGFAAHLLDGIGFHETFLHGTLGFLLFAASLQVNMMELSKHKTIIVLLATVSVILSTF
                     MIGFGTYGLTQLLDIPIPLEYCLVFGALISPTDPITALGILKTANAPKGLEMKIAGEA
                     LFNDGMGIVLFVVLLGLAVGGESQWTLSSAMIFFLRQGIGGVALGFVMGVLTARLLRT
                     IDNSEVAISLTLGIVAGGYAFAHSVMEVSGPICMAVAGLSVGNLLHDGNMSRHTLQRL
                     NAFWELLDDILNAILFVTIGLEFLSLSFRMDTAIASIGVIIIAMMARWLSVMLPVASV
                     SMFRKFSPNVVLIMTWGGLRGGISIALALTLPLGPIRDFILVITYAVVLFSIMVQGLT
                     LGGLVRRITKLKAFQEEYSKELRVSRVTLNMTKDALE"
     gene            2571..>4217
                     /locus_tag="PK389_02080"
     CDS             2571..>4217
                     /locus_tag="PK389_02080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22532.1"
                     /translation="MKLTPNRRLGFYLRSIPLNAWLEALYTDLCLADPTFPYRLSSNE
                     EAVVWEEIIAQSEVGKSLLKVSSTARLAQDAWLLSVQWRLPQKTLFQSEDNVAFSEWV
                     NDYQVRCKKEHWVDSATFVDQLIHAIDQKKLDLPVEMTLIGFEELTPQQTLFFEKVKA
                     AGSRVKSESLVSKFGNLRRLAVTNPEEELRCAVLKAKQWLNHHPEARIGIVVPALTEQ
                     RALVIRILEEAFTPVFFNVAAPVALRLYPLVDSALLVLRFFMEIPLEKFSRFLRSPFF
                     NNASLEQNIRAALDVMIRGFSETAFTLESAIKKMEVSMQQEPTLANFTGLADLQKLVL
                     MRTKIRGKQKAPFWRDQFLEILNCVGWPGDRQLTSEELEIIAQWQKVLMDYVSMERVL
                     GEHRYVDAVSHLQRLLQNTVYSPSAMGETRGSAPIQVLGILEALGMPFDYLWVTGLYR
                     EAWPPDPAPNPLIPLSLQRQYDLPRSSAARELKMARQFTERLSQGGKEVVFSYPSMIE
                     DRSVSVSPLIADLPEVTDRELGIDFDLPNLSNVHEITSSFF"
BASE COUNT         1129 a          760 c          985 g         1343 t
ORIGIN      
        1 aagcaatatt ttcgaaaaca gttctcgtgt tgaataaatt aaaatgctcg aagatcatgc
       61 cgatctggcg acgtgcttct cttattcccg ttgcagagag cgtgcttaaa tcacagctat
      121 cgataactac cgagccgcga gaagggggat ctaaaaaatt gattgtgcgg atcaaggcgc
      181 ttttaccagc gccggcatgg cctaggatac cgaaaatttc cccttgctcg atatttaagg
      241 taatgccttt cagtgtatga acaacacgat ttcctttttt gatgactttt tcgattccat
      301 tgagtgagat catagaatta tctctgaaat ttgaaccatt tcacttgagt ttaacaatag
      361 cgtgtaaaga caaaactttc aacgatttct atcgtgtctc agataataac atcagttaca
      421 tcgctgaata cggcagtaac acctttttgc agcagcgtct tcattctgtt cggatcgtta
      481 acagtataaa ccaaaacggg taaggctatt gattttgagg tggctttgtc tagaagtgct
      541 ggcgagaaat tttggttatc acaatggagc gaatcaaagc ccgcttctaa aatcttatcc
      601 agtgtgtttt cagtgggatt tcgaacgata taccctaagg gtaatttgtt atctaatgat
      661 cttaaaatct taaggctttt cagatcgaag ctcgaaacca agggtggtgg aagatccttt
      721 ggccacgcat tgtgcaagac atttaagaag tctgcaatgg cttcgataga gagtggttgt
      781 tcaaatactt ttagctctat gttagggtgc aacttaagat cagccaaaca agacaatgtg
      841 tcttgaaggg taggaacacg ttcattttta aactgggggt gaaaccagct gcctgcatcg
      901 agcgttttaa gtgtttgata gggcgtatca ataaccaatc ctgttccatt tgtcgtgcga
      961 tcgagctttt catcatgaaa gaccacccac tcctcactcg cgcagcgttg cgtgtcgaat
     1021 tccacccaac ttaaaccaaa ttgcgcagct tttttaaagc cagcgagcgt attttcgggt
     1081 gcttgagcac tcattccgcg atgtccgata attccgcgtt gaatggaggg aagattaaaa
     1141 aataagtttg acactaaact ttctccattt ttttccgttt aatttaggaa agatgtttgt
     1201 tacagtgtag gggactttta tggtttattg ctaataacat gattaggggt tagcgtgcgc
     1261 tcagagcaag tagtagggat tcttatgtct ctgacagcag tttcaagtta cctgaattat
     1321 aggttcgtga aactgccgaa aaccattggc ttaacgataa ttaccctcgt tttgtctttt
     1381 tttattgctg tgagtggtaa attcggatgg gatgtagaag ggtttgccgc acatctcttg
     1441 gatggtattg gattccacga aacatttctg catggcacct tgggatttct cttatttgcg
     1501 gcctcgctgc aagtcaatat gatggaatta tctaaacata aaactattat cgtgttatta
     1561 gcaacagtca gtgtgatttt atcgacgttt atgattggtt ttggcactta tggtttaaca
     1621 caacttttag atattccgat tccgctcgag tattgtttgg tgtttggcgc tttaatttca
     1681 ccgaccgatc cgattaccgc cctaggtatc ttaaagactg ctaatgctcc gaaaggactc
     1741 gaaatgaaaa tagcgggaga agccctcttt aatgatggta tgggcattgt gttatttgtg
     1801 gtgttattgg gcttagcagt tggtggagaa agtcaatgga ctctttcaag tgctatgatt
     1861 tttttcttac gacaaggtat tgggggtgtt gcattaggtt ttgtgatggg cgtattaact
     1921 gcgcgtttat tgcgaaccat cgataactcg gaagttgcga ttagtttgac attaggtata
     1981 gtggccggtg gttatgcctt tgcacattct gtgatggaag tttcgggacc tatctgtatg
     2041 gcagtagcgg ggctctctgt cgggaactta ttgcacgatg gaaatatgtc taggcatact
     2101 ttacagcgcc taaatgcttt ttgggaatta ttagacgata ttttgaatgc catattgttt
     2161 gtaacgatag gacttgaatt tttaagcctg agttttcgaa tggataccgc tatcgcatcg
     2221 ataggtgtga tcattattgc aatgatggcg cgttggttaa gtgttatgct tcctgttgct
     2281 tctgtatcga tgtttcgtaa gtttagcccc aatgtcgttt taattatgac ttggggtgga
     2341 ttaagaggag gcatttcgat agctctcgct ttaacgttgc ctttgggtcc tatccgcgat
     2401 tttattctgg tgattactta tgcagttgtt ctattttcaa ttatggttca aggtttaacg
     2461 ctgggtgggt tggttcgacg tattacaaag ctaaaagcat ttcaagaaga gtattctaaa
     2521 gagttacgtg tttctagagt tacacttaat atgacgaagg atgcgttaga atgaagttaa
     2581 cgcctaatcg gcgtttgggg ttttatttac ggagtattcc gcttaatgcg tggttggaag
     2641 ccctatatac tgacttgtgt ttggcggatc caacctttcc ttatcgatta tcaagcaacg
     2701 aagaagcggt tgtttgggaa gaaatcattg cgcagtctga agtcggtaaa tccttattaa
     2761 aggtatccag tacggcacgt ttagcccaag atgcctggtt gctgtcggtt cagtggcgtt
     2821 taccacaaaa aacgctgttt caatcggaag ataatgtggc cttttctgaa tgggtaaacg
     2881 actatcaggt gcggtgtaag aaagagcatt gggttgattc tgcaacgttt gtcgatcagt
     2941 taattcatgc tatagatcaa aaaaaattgg atttgcctgt cgagatgact ttgataggtt
     3001 ttgaagaact aacaccacag cagacacttt tttttgaaaa agtcaaagca gcaggctcgc
     3061 gtgtaaaatc agagtcgctt gtctcaaagt ttggaaactt aagaagactt gccgttacca
     3121 atccagaaga agaattaaga tgtgcggttt taaaagcaaa gcaatggtta aatcatcatc
     3181 cagaagcgcg tattggtatt gttgtgccag ctttaacaga acaacgagct ttagttattc
     3241 gaatactaga agaagctttt acacctgtgt tttttaatgt ggcagcgcct gtcgcattaa
     3301 gattatatcc gctcgttgat tcagctttat tagtattgcg tttttttatg gaaattcctt
     3361 tagaaaaatt cagtcgtttt ttacgctcgc ctttttttaa taatgcgtca ctagaacaaa
     3421 atattagagc tgccttggat gttatgattc ggggtttttc tgaaacagcc tttacactgg
     3481 aaagcgcaat taaaaaaatg gaagtgagca tgcagcaaga accaacgctt gccaatttta
     3541 caggattggc ggatttgcag aaactggtat tgatgcgaac aaaaatacgt gggaaacaaa
     3601 aggcaccttt ttggcgcgat cagtttttag agatactgaa ttgcgtaggt tggccaggtg
     3661 atcgccaatt aacatctgaa gaattagaaa taatagcaca gtggcagaag gttttgatgg
     3721 actatgtttc tatggaacgt gtgttaggag aacatcggta tgtggatgca gtttctcatt
     3781 tgcagcgatt actgcaaaat acggtgtatt ctccgagtgc aatgggggaa acacgcggta
     3841 gtgcgcccat tcaagttttg gggattttgg aagcacttgg aatgcctttt gattatcttt
     3901 gggtaaccgg cttgtatcgt gaagcatggc cgccggatcc cgcgcccaat cccttgatac
     3961 ccctatcgct acagcgtcag tatgatttac cacgtagtag cgctgcccga gaattaaaaa
     4021 tggctcgaca atttacggag cgattatcac aaggcggaaa agaagttgtt ttcagctatc
     4081 cgtcgatgat tgaagaccgt tctgtttctg tcagtccttt aatagcagat ctcccagaag
     4141 tgacggatag agaactaggg atagattttg atttgcctaa tctaagcaat gtgcacgaaa
     4201 taacttcctc ttttttt
//
LOCUS       DAORVT010000069         4160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_16359_length_4160_cov_3.467722, whole genome shotgun sequence.
ACCESSION   DAORVT010000069 DAORVT010000000
VERSION     DAORVT010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16359_length_4160_cov_3.467722"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /gene="murD"
                     /locus_tag="PK389_02085"
     CDS             complement(<1..977)
                     /gene="murD"
                     /locus_tag="PK389_02085"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HQY22533.1"
                     /translation="MNNNTDTVIIGLGETGFSCVQHYAAQGIPIIVMDSRENPPKLAA
                     LKKSYPHIPVYTGVFPKALLAKAKTVVLSPGISQDHPDLIHSFLPQAEIIGDIELLAR
                     STTAPVVAITGSNGKSTVTTLVGEMAKAAGIRVGVGGNLGTPVLALLKEKPDLYVLEL
                     SSFQLETTHSLKPVVATVLNICPDHLDRYASVEAYQAAKHRIFKNCEAIVYNREDPQL
                     PGIMGSDPIMKTISFGTDAPLENHYGLIKNKEVLCLARGKELLLPASKLKISGLHNLA
                     NALAALALGESIALPMASMLSVLQSFTGLPHRAEWVADYLGVPWINDSKG"
     gene            complement(1044..2126)
                     /gene="mraY"
                     /locus_tag="PK389_02090"
     CDS             complement(1044..2126)
                     /gene="mraY"
                     /locus_tag="PK389_02090"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008045924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HQY22534.1"
                     /translation="MLIWLAEYLTQYYRAFSVFEYITLRAIVASLTALIITLFVGPPM
                     IRWLAQYKVGQVVRNDGPQSHFSKAGTPTMGGAMIILAIAIATLLWSNLRNPYVWIVL
                     TVMVGFGMIGWCDDYRKLMRRNSKGLSARLKFLLQSIVGLAAAIFLYMKATTPAETSL
                     LIPFFKNIVVPLGFFYIILTYLVIVGTSNAVNLTDGLDGLAIMPIVLVGGALGIFAYV
                     TGHVKFATYLSVPYIPGVEEITVFCGALVGAGLGFLWFNAYPAQVFMGDVGSLGLGAA
                     LGIIAVLVRQELVLFIMGGVFVAETVSVILQVGSFKLTGRRIFKMAPIHHHFELKGWP
                     EPRVIVRFWIITVVLVLFGLATLKLR"
     gene            complement(2120..3523)
                     /gene="murF"
                     /locus_tag="PK389_02095"
     CDS             complement(2120..3523)
                     /gene="murF"
                     /locus_tag="PK389_02095"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HQY22535.1"
                     /translation="MSTLTLAFAAESMGAANISNIPPSPPVKGGANNLIFKDVKTDSR
                     QVSPGTLFIAIPGAKVDGHDFIHEAEARGAVGVVISRPIDTTLPTLLVPDTVFALGQL
                     AKAYRQSFQIPMVALTGSAGKTTTKEMLTHILAEQGSVLSTQGNLNTEIGVPLTLLGL
                     LPTHQYAVIEMGARKQGDIAYLMRLATPRVALITNAGIAHQEVFGSKSAIAKAKGEIF
                     ECLQSQGIALINADDTYADYWRGLLKAGQHIISFGMTHSADVFGKNIILEPTLSQFEL
                     NTDIGTITIQLQIPGLHMVQNAIAAAAAARALNISLLKIKTGLEKFVSVAGRLQFKKG
                     LKGVSIIDDTYNANPISIRAALTVLSKLPGRQIFVMGDMVEMGEDAEKLHHEMGLQAK
                     SLGIDKMFGVGPMTALAIQAFGQNAMHFSDKSLLIQALNSEISSDTTILIKGSRFMRM
                     EEVVFALTTNCQETHTC"
     gene            complement(3511..>4160)
                     /gene="murE"
                     /locus_tag="PK389_02100"
     CDS             complement(3511..>4160)
                     /gene="murE"
                     /locus_tag="PK389_02100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramyl-tripeptide synthetase"
                     /protein_id="HQY22536.1"
                     /translation="IRATVKTPWGEGELRSHLLGLFNVSNILAALTAVCLQGVPLNAV
                     LKAIKAISTAPGRMMRFGGNTEMPLVIVDYAHKPDALALVLEALRGHCVGELWCVVGC
                     GGDRDRGKRPVMAAIGERLSDHLYLTQDNPRTEDPTQIMAEMIQGLKNPTKVIVEYDR
                     AKAIDSAIKNAKAEDIILVAGKGHETYQIIGTEKMPFSDKLVVEAALERRMLCLL"
BASE COUNT         1243 a          989 c          781 g         1147 t
ORIGIN      
        1 cctttggaat cgttaatcca aggcacccca agataatcag caacccactc tgcacgatgt
       61 ggcaatcctg taaaagattg taataccgat aacatcgatg ccatgggcaa tgcgatgctt
      121 tcgccgagtg ccaatgccgc taaggcatta gcaagattat gtaagcccga tatttttaat
      181 tttgatgcgg gtaataacag ttcttttcct ctggctaagc acaacacctc tttgttttta
      241 attaaaccat agtgattttc taaaggcgca tccgttccaa agctaatggt tttcataatg
      301 gggtcagacc ccattattcc cggcaactgc ggatcttctc ggttataaac aatcgcttca
      361 caatttttga aaatgcgatg ttttgcggct tgataagcct cgaccgaagc ataacgatcg
      421 agatgatcag gacaaatatt taaaaccgtt gcaacaacgg gttttagcga atgcgttgtt
      481 tctaattgaa agctcgataa ttcaagtaca tataaatctg gtttttcttt taaaagtgcc
      541 aatacgggcg taccgaggtt tccaccaacg cctacacgaa tacccgcggc tttcgccatt
      601 tctcccacca acgttgtcac cgtacttttt ccatttgatc cggtaatggc aacaacggga
      661 gcggtcgtac ttctcgctaa taactcgata tcgccaatga tctctgcttg aggtaaaaat
      721 gaatggataa gatcagggtg atcttgagaa attcccggac ttaaaaccac tgttttggct
      781 tttgctaata gcgctttagg aaatacgcct gtataaacgg gaatatgtgg atagcttttt
      841 tttaatgctg ctaatttagg tggattttcg cgtgaatcca tcacgataat aggaataccc
      901 tgtgcagcat aatgttgaac gcaagaaaag ccggtttcgc cgagaccgat aatgactgtg
      961 tctgtgttat tattcattta tccctcatcc gccctgcgga caccttctcc caaagggaga
     1021 aggagaaacg taaataagct aaattagcga agtttcagtg ttgctaatcc aaataaaacc
     1081 aataccacgg taataatcca aaaacgtaca attacgcgtg gttctggcca cccttttaat
     1141 tcaaagtgat gatgaatagg tgccatttta aaaatacggc gaccggttaa tttaaatgat
     1201 ccaacttgta atatcacaga cacggtttct gccacaaaga caccacccat aataaataat
     1261 actaattctt gtcgcactaa aacagcaata atgccaagtg ctgcgcctaa accgagcgaa
     1321 cccacatctc ccataaagac ttgcgcagga tacgcattaa accataaaaa tccaagacct
     1381 gcaccaacca atgccccgca aaaaacggta atttcttcga ccccgggaat ataaggaaca
     1441 gataaatacg tcgcgaattt cacatgtccc gtcacgtagg caaaaatgcc caatgcaccg
     1501 ccaactaaca caatcggcat aatggctaat ccatccaagc catccgttaa attaacggca
     1561 ttactcgtgc caacgatcac aagatatgtc aaaataatat aaaaaaaacc cagtggcaca
     1621 acaatattct taaaaaatgg gattaacaaa ctggtttcgg ctggtgttgt ggctttcatg
     1681 tataaaaaaa tagctgctgc taaacccact atcgattgta acaaaaattt caaccgcgca
     1741 gataaacctt tagaattgcg tcgcattaat tttcgatagt cgtcgcacca gccgatcatg
     1801 ccaaacccaa ccataacggt taacactatc cacacatagg gattacgcaa attactccag
     1861 agcagtgttg caatagcaat cgcaaggatg atcatggcgc cacccatcgt tggggtaccg
     1921 gctttactaa aatgactttg aggaccatca ttgcgtacga cttgtccaac tttatattgc
     1981 gccaaccagc gaatcatcgg aggtccaacg aataaagtaa taatcaatgc cgtcagtgat
     2041 gccacaattg cccttagcgt aatatattca aacacgctaa atgcgcgata atactgggtt
     2101 aaatactctg caagccaaat taacatgtgt gcgtttcctg gcaattcgtt gttaatgcaa
     2161 aaaccacttc ttccattcgc ataaaacgcg aacctttaat taaaatcgtt gtatctgaac
     2221 tgatttccga gttcaatgcc tgaattaata aagatttatc agaaaaatgc attgcattct
     2281 ggccaaatgc ctgtattgct aatgccgtca tgggacctac accaaacatt ttatcaatac
     2341 ctaaactttt agcctgaagc cccatctcat gatgcaattt ctctgcatct tctcccatct
     2401 caaccatatc acccatcaca aaaatctgtc tacccggtaa ttttgacaat actgtcaacg
     2461 cagcacgtat agaaatcgga tttgcattat aggtatcatc gataatcgat acgcccttta
     2521 accctttttt aaattgtaaa cgcccagcaa ctgacacaaa cttttctaat cctgttttaa
     2581 tcttcaacaa agaaatattc aatgcacgtg cagcagctgc tgcggcaatg gcattttgta
     2641 ccatatggag acccggtatt tgcagctgaa ttgtgattgt accgatatcc gtattcaatt
     2701 caaattgtga taacgtgggt tctaagataa tattttttcc aaaaacatcc gccgaatgcg
     2761 tcataccaaa gctaataata tgttgacccg cttttagcaa accacgccaa taatctgcgt
     2821 atgtatcatc tgcattaatc agtgctatac cctgtgattg taagcattca aaaatttcgc
     2881 cttttgcttt tgcaatagcc gatttactgc caaaaacctc ttggtgtgca ataccggcat
     2941 tggtgatcaa tgcaacacgg ggtgttgcta agcgcattaa ataggcgatg tcaccttgtt
     3001 tacgcgcccc catttctatc acagcatact gatgcgttgg caataaacct aagagcgtta
     3061 aaggcacacc gatttctgta tttaaattgc cttgggtgga taataccgaa ccttgttctg
     3121 ccaaaatgtg tgttaacatt tctttagtag ttgttttacc cgcactgcct gtcagtgcca
     3181 ccatgggtat ttgaaaagac tgtctatagg cttttgcaag ctgccctaag gcgaaaaccg
     3241 tatctggtac caacaaggtt ggtaatgttg tatcaattgg acgactaata acaacaccca
     3301 cagcaccacg cgcttcggct tcgtgaataa aatcatgacc atcgacttta gcacccggaa
     3361 tcgcaataaa taaagtgcct ggtgatactt gacgagaatc tgttttgaca tctttaaaaa
     3421 taagattatt agctcccccc ttcacagggg ggctgggggg gatgttggag atgttggcgg
     3481 cacccatgga ttcggctgca aacgctaaag ttaaagtaga catagcattc tccgttctaa
     3541 tgctgcctct acaacaagct tatctgaaaa tggcattttc tctgttccga taatttgata
     3601 ggtctcgtgc cctttgcctg caaccaggat aatgtcctca gcttttgcat ttttaatagc
     3661 cgagtcaatc gctttagcac gatcatattc aacaatcact ttagtcgggt ttttaagtcc
     3721 ttgtatcatc tctgccataa tttgcgtggg atcttccgtt ctcggattgt cttgggttaa
     3781 atataagtga tcacttaagc gttcgccaat ggctgccatt acagggcgtt ttccgcgatc
     3841 gcgatcgcca ccacagccaa cgacacacca caattcaccc acacaatgtc cccgcaaggc
     3901 ttctaaaacc aaagctaagg catccggctt atgcgcataa tcgacaatga ccaaaggcat
     3961 ctctgtattt ccgccaaagc gcatcatccg cccaggagcc gtactgatcg ctttaattgc
     4021 ttttaatact gcattcagcg gaacaccctg taaacagacc gccgtcagcg ctgccaaaat
     4081 attgctgaca ttaaaaagtc ctagcaaatg agagcgcaat tcaccctcgc cccaaggcgt
     4141 ttttacagtg gcacgaatgc
//
LOCUS       DAORVT010000070         4151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_16430_length_4151_cov_2.824951, whole genome shotgun sequence.
ACCESSION   DAORVT010000070 DAORVT010000000
VERSION     DAORVT010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16430_length_4151_cov_2.824951"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1397
                     /locus_tag="PK389_02105"
     CDS             <1..1397
                     /locus_tag="PK389_02105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQY22537.1"
                     /translation="LKKMTSKTEYNVIVIGAGMAGLSAAQHLADLGHTVLVLEAKNRV
                     GGRIHTQEMQDGLPIEMGANLLLNPGTTEQPNPLLPLIRHLNLTTMPIDSIPSTTFKA
                     EGYFENATKLINEAKRFPWKTWPSLGEVLKIQDLNFHSHLKLNSTEFIGKQALIHMIE
                     QQTGTLPHNVSLLELMHFGTDATFYDSDGFRGSAEATFINGGYQQLPEAMAKKAIATG
                     KVKILLSTPVEAVHYHNSDTTQVIATNGEKYTAKRILCAVPIGVLQNQSIHFFPKLSP
                     EKQQLIKHLKVGFHNKVVLEFKKVFWPIDTHFLFPGSKHYHHFPEYLNLFHFSNQKTP
                     ALVCNFYASEACFKENTDDHIVEKALLPLQEAYGKTAIALKNAFVTHWESDPYALGST
                     SCYGLQCESRELRNFEKPEQGGLFFAGAHTIANKNRETVQGAYISGIRAALDVNHHLK
                     NAELRGRTQRSAPT"
     gene            complement(1407..2150)
                     /locus_tag="PK389_02110"
     CDS             complement(1407..2150)
                     /locus_tag="PK389_02110"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQY22538.1"
                     /translation="MLNMSKHWKRLRVWWDSPLGLAFLKSEEEQVKKMLPQLFGYHLL
                     VLGEPVFLQCVQDSAITHRVAIHPSASFSETCSTLRSRHDKLPVISDGVDLIYLAQCL
                     AFIKNPHEVVREAFRVLIPEGHVIISNFNPWSVWGLWRWCLHYIKRVPWDGHFISVTR
                     LKDWLALLGFDVLEVRRYFFRPPISHPVILQRLEWLEKLGRWCWPFFGGGYVLLAQKR
                     IMTLTPIRPFFVTERTVLVPSGIEPVRSE"
     gene            2366..3310
                     /locus_tag="PK389_02115"
     CDS             2366..3310
                     /locus_tag="PK389_02115"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HQY22539.1"
                     /translation="MSTLIISSNLCGCDRTNNTQNISKKQKTPAENHKQLWNRIRDNY
                     DIASKEIETHKQPQIQKYVKHYTEKEKALTKMSTQATPYLFHIVEELEQREMPGELAL
                     LPMIESAFRPEATSNRGAAGLWQFIPSTGRYFGLKQNAWYDERRDIRTSTSAALDYLA
                     FLHETFDHNWMLALAAYNAGEGTVRRAIKKNLKLGKPTSFWDLQLPKETREYVPKFLA
                     LAEIIGQPEKHDILLPDIENKPYFSPVFFNKSLNFQQAANLAKIHITELKRLNPGYKR
                     SHIHTKASPEFLLPIANKAIFEYNFANKDKKVKSEHEH"
     gene            3300..4115
                     /gene="ttcA"
                     /locus_tag="PK389_02120"
     CDS             3300..4115
                     /gene="ttcA"
                     /locus_tag="PK389_02120"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HQY22540.1"
                     /translation="MSIEVSDIIEKPLQVFRSKVEKKLLHYTGKAIADYKLIETGDRV
                     MVCLSGGKDSFTLLSLLKLLRMRSGNKFELFSFTLDQAQPGWIDTALRAFLDKQGVPY
                     EILNRDTYSIVKEKIPEGKTYCGLCSRLRRGIIYRYAEENGFNKIALGHHRDDLIRSL
                     LMSMMYNGEIRSMPPKLLSDSKKNIVIRPMVYCQEKDIITYAKERKYPIIPCNLCGAQ
                     ENLVRVKIKKLIDELALDNPKVPSNLLSALQNIRPSQLMDHDLWDFKGLIREA"
BASE COUNT         1366 a          900 c          774 g         1111 t
ORIGIN      
        1 tactgaaaaa aatgacgagt aaaacagaat acaatgtaat cgtgattggt gcgggtatgg
       61 cgggactctc ggccgcacaa catctcgcag atctgggtca taccgttctc gtgctagagg
      121 ccaaaaatcg cgtcggtgga cgtattcata cgcaagaaat gcaggatggt ctccccatag
      181 aaatgggagc caatctgctg ttaaatccag gtacaacaga acaaccaaac cctttgctac
      241 cgttaattcg tcacctcaat ctaacaacca tgcccatcga ttccatccct tcaacaacat
      301 ttaaggcaga aggctatttt gagaatgcga cgaaattgat aaatgaagcc aaacgattcc
      361 cttggaaaac atggccttct ttaggagaag ttctaaaaat acaagactta aattttcaca
      421 gccaccttaa actaaacagc actgaattta tcggcaaaca agcgcttatc catatgatcg
      481 aacaacaaac tggcactttg ccacataacg tctcgctttt ggaattgatg cactttggta
      541 cagatgcaac attctatgat tctgatggtt ttagaggatc tgcagaagcg acatttatca
      601 acggtggcta tcaacaatta cccgaagcta tggcaaaaaa agcaatagcg acaggcaaag
      661 ttaaaatctt gttgagcaca cctgtagaag ctgtgcatta tcataatagt gatactacac
      721 aggttatcgc gacaaatggc gaaaaatata ccgcaaaacg tatattgtgt gctgttccta
      781 tcggtgtttt acaaaatcaa agtattcatt tttttcctaa actctctcct gaaaaacaac
      841 aattaataaa acatctaaaa gtcgggtttc ataataaagt tgttttggaa ttcaaaaaag
      901 ttttttggcc aatcgacacc cactttcttt ttccaggtag caagcattat catcactttc
      961 ctgaatacct taatttattt catttttcaa atcagaaaac acctgcctta gtctgcaact
     1021 tttatgcatc cgaagcctgt tttaaagaaa acacagacga tcatatcgtt gaaaaagcat
     1081 tgttaccgct gcaagaggct tatggcaaaa cggctatcgc tttaaaaaat gcttttgtaa
     1141 cccattggga atctgatcct tatgctttag gcagtacttc ttgttatggt cttcagtgtg
     1201 aaagtagaga actgaggaat tttgaaaaac cagaacaggg cggattattt tttgcaggag
     1261 cacacacgat tgccaataaa aatcgagaga ctgttcaagg tgcttatatt tcaggcatac
     1321 gcgcagcact tgatgtcaac caccatttga aaaatgcgga actacgaggg cggacacagc
     1381 gatccgcccc tacctaaaac tatccattat tcggatcgta caggctctat cccactcggc
     1441 acgaggactg tgcgctctgt tacaaaaaaa ggtcgaatcg gtgttaaagt cattatccgt
     1501 ttctgtgcta ataacacata tcctccacca aaaaatggcc aacaccagcg tcccagtttt
     1561 tctagccatt ctaaacgctg taatataaca gggtgtgaaa tgggtggtct aaaaaaatag
     1621 cgtctgactt ctaaaacatc aaatcccaat agcgccaacc aatcttttaa gcgtgtaacc
     1681 gaaataaaat gaccatccca aggtacacgt ttaatataat gcagacacca tcgccaaagt
     1741 ccccaaacac tccatgggtt aaaattagaa ataatcacat gaccttcagg gatcaaaaca
     1801 cgaaacgctt cacgcacaac ttcatgggga ttttttataa atgccaaaca ctgcgccaaa
     1861 taaatcaaat caacgccatc cgaaataacc ggtaatttat cgtgtcttga tcttaaggta
     1921 ctacaggttt cggagaagct tgcagaggga tggatcgcaa cacgatgtgt tatggcgctg
     1981 tcttgtacgc attgtaaaaa aaccggctcc cccaatacca acagatgata tccaaacaat
     2041 tgaggcagca tttttttgac ttgctcttct tccgacttta aaaaagccaa tcccaaagga
     2101 gagtcccacc agacgcgtag acgtttccaa tgcttagaca tattcagcat acttgctttt
     2161 tcccctgacc tcacccggcg ctacgcgcca ccctcgtggc cggtttgtat cctaattgta
     2221 actaaagcca atttatatag cacgcgcagc gtacattgct ggctgatcat ttacccggtt
     2281 gacgaaatct gcttaagccc gtaatttaac tctgatgata gattttcaaa acaagagaat
     2341 taagtttctt aactatatat ttatattgtc taccttgatc atcagctcaa acctctgtgg
     2401 atgtgatcgc acaaacaata cgcaaaatat ttccaaaaaa caaaagacgc ctgctgaaaa
     2461 tcataaacaa ctttggaatc ggatccggga caattatgac atagcctcta aagagataga
     2521 aacacacaaa caacctcaaa tccaaaaata tgttaaacac tatacagaaa aagaaaaagc
     2581 attaacaaaa atgagtaccc aagcaacgcc ttatttattc catatcgtag aagaacttga
     2641 acaacgcgag atgccgggcg aattggcttt attgcccatg atagagagcg cttttcgacc
     2701 agaggcaact tctaaccgag gcgctgcggg tctttggcaa tttatcccga gtacaggaag
     2761 gtactttgga ttaaaacaaa atgcttggta tgatgaacgt cgagacatta gaacttcaac
     2821 cagtgctgct ttggattatc tcgcgttttt acacgaaaca tttgatcata actggatgtt
     2881 agcattagct gcttacaatg caggcgaagg cactgtccgt cgtgccatta agaaaaactt
     2941 gaaactcgga aaaccgacct ctttttggga tctccagtta cccaaagaaa cacgagaata
     3001 tgtccctaaa tttttagcct tggctgaaat cattggacaa cctgaaaaac acgatatcct
     3061 actacccgac atcgaaaaca agccctattt ttcacctgtt tttttcaata aatccttaaa
     3121 ttttcaacaa gcggcaaact tagcaaaaat tcacatcaca gaattaaagc gcttaaaccc
     3181 cggttataaa cgttcgcaca ttcacaccaa agcctctcca gagttcttat tacccattgc
     3241 gaataaagca atatttgagt acaattttgc aaacaaagac aaaaaggtca aatctgaaca
     3301 tgagcattga agtttcggac atcattgaaa agccccttca agtatttcgt tctaaagtcg
     3361 aaaaaaaatt gctacattat acaggcaagg caattgcaga ctataagcta attgaaacag
     3421 gcgatcgagt gatggtctgt ctgtcgggtg gaaaagattc ttttacgcta ttgtctctgc
     3481 tgaagctgct ccgaatgcgg tctggcaata aatttgaatt attttctttt acactagatc
     3541 aagcacaacc tggatggatc gataccgcac ttcgcgcttt tttagataaa caaggtgtcc
     3601 cttatgaaat tttaaatcga gacacctact caatcgttaa agaaaaaatt ccagaaggta
     3661 aaacctattg cgggctttgt tcgcggctcc gacgcggtat tatttatcga tacgcagaag
     3721 aaaatgggtt taataaaatc gcgcttgggc atcatcgcga tgatctgatc cgttcgttat
     3781 taatgtcgat gatgtacaat ggtgaaattc gctctatgcc accgaaacta ttaagcgata
     3841 gcaaaaaaaa tattgtgatc cgtcccatgg tttactgcca agaaaaagat attattacct
     3901 atgcaaaaga gcgaaaatat ccgattattc cgtgtaatct ttgcggtgca caagaaaatc
     3961 tcgtgcgcgt aaaaattaaa aagttgatcg atgaattggc actagataat ccaaaggtgc
     4021 ccagcaattt gctaagcgca ctgcaaaata ttaggcctag ccaattgatg gatcatgatc
     4081 tatgggattt taaagggttg ataagagaag cttagcctct ctgcaataaa aatttattgt
     4141 ggcgaaggtg c
//
LOCUS       DAORVT010000071         4129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_16595_length_4129_cov_3.624202, whole genome shotgun sequence.
ACCESSION   DAORVT010000071 DAORVT010000000
VERSION     DAORVT010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16595_length_4129_cov_3.624202"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="PK389_02125"
     CDS             complement(<1..291)
                     /locus_tag="PK389_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22541.1"
                     /translation="MSRKGKNLGFVRDFESGKRDDDLQLLSTIEKQVQGVLGEVQYIT
                     ETIETHKKVAPVEDIEAKIKKSYEKIKKLMNSDNADLNMALEGDAKRRTSGGI"
     gene            complement(456..827)
                     /locus_tag="PK389_02130"
     CDS             complement(456..827)
                     /locus_tag="PK389_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQY22542.1"
                     /translation="MAIKKLKKSNKAGNQKQLLGRKKQTRIEILRSIADNTGLAKVQI
                     ESVFSALAVLLHAHMEKRGSGEFTVPAMGIKVRRVKKKATKSRTMVSPLTGQEVLIPG
                     KPARMAVKLSALKPLKLAVLE"
     gene            complement(904..3198)
                     /locus_tag="PK389_02135"
     CDS             complement(904..3198)
                     /locus_tag="PK389_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020410977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HQY22543.1"
                     /translation="MLNNTIITRQIAVELNVRPEQVESAVQLLDAGDTVPFISRYRKE
                     ATGGLTDSDLRMLEERLAYLRELKERRVAILKSIEEQGKLTPELALAIQGADSKARLE
                     DLYLPYKQKRRTKAQIAREAGLEPLAQALLDDPTLEPEKTAEAYLNVDLNIPDIKAAL
                     EGARFILMEQFSEEANLLGLLRDKLWAEGTLTSQVVAGKEEAGEKFRDYFQYSELLKK
                     VPSHRMLAILRGRHETFLQVTIACDATGFEQMIARQFKVEDLGRKSDAWLLETVRWAW
                     RVKLYIQLELELVSRLRERSEAEAIQVFATNLRDLLMSAPAGSRATLGLDPGFRTGVK
                     VAVVDATGKVVQHSTIYPHAPKNAWDESIEVLATLCRLHKVELISIGNGTASRETDQL
                     AIDLIKKYPDLHLKKIVVSEAGASVYSASKLASEEFPDLDVSIRGAVSIARRLQDPLA
                     ELVKIDPKAIGVGQYQHDVSQSRLAHSLESVVEDCVNAVGVDVNTASVPLLTRVSGLN
                     PTLAKNIVAFRDDHGAFKNRQELTKIPRLGPKTFEQAAGFLRILAGENPLDASAVHPE
                     AYPVVEKVLLKTQKNMVDIIGNKQFLSTLRPEEYTDEQFGVPTVTDIFLELEKPGRDP
                     RPEFKAAAFNQTVQTLNDVKPGMILEGVVTNVANFGAFVDIGVHQDGLVHISALADKF
                     VQNPRDIVKAGDIVTVKVLEIDVKRKRIALTLRLHDEARAHPTASRDNASKEVQRFSV
                     AKKQTEIKNSLGTLLEAAFKKKES"
     gene            complement(3200..>4129)
                     /gene="glmS"
                     /locus_tag="PK389_02140"
     CDS             complement(3200..>4129)
                     /gene="glmS"
                     /locus_tag="PK389_02140"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020190289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HQY22544.1"
                     /translation="CGTSYHAGMIGRLWLENLAGISCHVEIASEFRYRKRVLLPNTLL
                     ICISQSGETADTLAALRSTQGENYCGVLSICNVPDSSLVRESALVFLTNAGPEIGVCS
                     TKAFTTQLVALFLLTLVLARRRGLEREKEILLIQQLVELPDIIQGCLASAKAIQTIAT
                     LFQNKSHALFVGRGLQYPIVIEGALKLKEISYIHAEAYPAGELKHGALALVDKKMPVV
                     VVAANDGLLEKLKSNIQEVRARGGELLIFTTKSSGIEEAPGIHVLEIPEVPELLGPIV
                     YTVPLQLLAYYVAVLKGTDVDQPRNLAKSVTVE"
BASE COUNT         1106 a         1012 c          757 g         1254 t
ORIGIN      
        1 aataccaccc gaagtgcgcc gcttggcatc gccttctaat gccatattga gatcagcgtt
       61 atcggaattc attaattttt taattttttc gtaagacttt tttatttttg cttcgatatc
      121 ttctacgggt gcaacttttt tgtgcgtttc tatggtttct gtaatatatt gcacctcacc
      181 cagcacacct tgcacttgct tttcgatagt gctaagcagc tgaagatcgt cgtctcgctt
      241 ccccgactcg aaatcacgca caaaccctaa atttttccct tttcgagaca ttgcactata
      301 ctctcttaaa cgccttctgc cagctcatat tctaccatat tgacaagaat atattattat
      361 taggcaagca attctggcaa gagttcagga gctaaaccca gcaatccaca ttaaataaac
      421 gccaaaacgt gcaatgtccc aaattttagc gcctcttatt ctaatacggc caatttcaaa
      481 ggtttcaatg cgcttaattt aaccgccatt cgcgcaggct tgccagggat cagtacttct
      541 tgcccagtta aaggactgac cattgttcgt gacttcgtcg cttttttctt aacacggcgc
      601 accttaatgc ccatcgcagg cactgtaaac tctcctgatc cgcgcttttc catatgcgcg
      661 tgtaatagca ccgcaagcgc cgaaaacacc gattcaattt gcactttagc caagcccgta
      721 ttatcggcaa tcgaacgtaa aatttcgatt cgtgtttgtt tttttcttcc taacaattgc
      781 ttttgattac ctgctttgtt tgattttttc aattttttaa tcgccatacc actctccaat
      841 gactttattg tagaaacagt cggcgattat gcctatctct cgcaaggata tcaagtcgtg
      901 tttttaggat tcttttttct taaatgcagc ctcgagcaat gtccccaaag aattttttat
      961 ttcagtttgt ttttttgcaa cagaaaaacg ctgaacttct ttggacgcgt tatctcgaga
     1021 tgccgttgga tgcgctcgtg cttcatcgtg taatcttaaa gtcagcgcaa tacgcttgcg
     1081 ttttacatct atctctaaaa ctttgacagt tacaatatca cctgccttaa caatatcgcg
     1141 tggattttgt acaaacttat ctgccaacgc agaaatatgc accaatccat cttgatgaac
     1201 acctatatcg acaaatgcac caaaattagc aacattagtc acaacgcctt ctaaaatcat
     1261 tcccggtttt acatcattca aggtttgtac ggtttgatta aaagccgctg ccttaaactc
     1321 tgggcgtgga tctctgccag gtttttcgag ttcaagaaaa atatcggtta ccgttggcac
     1381 tccaaattgt tcatctgtat attcttcagg acgcaatgtg cttaagaact gcttattgcc
     1441 aataatgtca accatgtttt tctgggtttt taacaaaacc ttttctacaa cgggataggc
     1501 ttcaggatga accgctgaag cgtctaatgg gttttctccc gcaagtatcc gtagaaatcc
     1561 agccgcctgt tcgaatgttt taggccccaa tcttggaatc ttcgtgagtt cttgtcgatt
     1621 tttaaatgcg ccatgatcat ctcgaaatgc cacaatattt tttgctaatg ttggatttaa
     1681 acctgagaca cgcgtcaaca acggcacaga ggcagtattg acatcaacac ctaccgcgtt
     1741 cacgcaatct tcaaccaccg attcgagtga atgcgccaag cgactttggc taacatcgtg
     1801 ttgatattgc ccaacgccaa tggctttagg atcgatttta actaattcag ccaacggatc
     1861 ttgtaatcgt cttgcaatag aaaccgctcc acgaatactg acatccagat ctggaaattc
     1921 ttctgaggcc agtttagaag cagagtaaac agatgcgccc gcttcagaaa ccacaatttt
     1981 tttaagatgt aaatctgggt attttttaat gagatcaatc gccaattgat cagtctcgcg
     2041 agaagctgtt ccatttccaa tgcttatcaa ttctacttta tgtaaacggc acagcgttgc
     2101 caagacttca atggattcgt cccacgcgtt tttaggcgca tgaggataaa tagtactgtg
     2161 ttgtacaacc ttgcctgtgg catccaccac agccacttta acgcccgttc taaagcccgg
     2221 atctaatccg agcgttgcgc gactacctgc aggcgcactc attaataaat cgcgtaaatt
     2281 cgttgcaaaa acctgtatgg cctcggcctc tgagcgctct cttaaacgac taacaagctc
     2341 taattccaat tgtatatata gcttaacacg ccatgcccaa cgaaccgttt ctagcaacca
     2401 cgcgtcgctt tttcgaccca aatcttcaac tttaaactga cgcgcaatca tttgctcaaa
     2461 gccagttgca tcgcacgcga ttgtcacttg caaaaatgtc tcatggcgtc cgcgtaaaat
     2521 tgccagcatg cgatgagaag gcactttttt caacaattct gaatattgaa aataatctct
     2581 aaatttttca ccggcttctt ctttgcctgc aaccacttga ctggttaatg tgccttctgc
     2641 ccacaattta tcacgcagca aaccaagcag attagcttct tctgaaaatt gttccataag
     2701 aataaaacgt gcaccttcta gtgcagcctt tatatctgga atatttaaat ccacattgag
     2761 ataagcttca gcggtttttt ctggttctaa tgtcggatcg tccaataaag cctgtgctaa
     2821 tggctctaat cccgcttctc gcgcaatttg cgctttcgtg cgacgttttt gtttgtacgg
     2881 caaatagaga tcttctaacc gtgctttgga atccgcacct tgaatcgcta atgctaattc
     2941 aggtgttagt tttccttgtt cttcgatgct ttttaatata gcgacgcgac gctctttaag
     3001 ttctctcaga tacgctaaac gttcctcgag catacgaagg tcgctatctg ttaatccacc
     3061 ggtcgcttct tttcgataac gtgaaataaa cggaacggtg tcgccggcgt ccaagagctg
     3121 aaccgctgat tccacttgct cgggccgaac gtttaactca actgcaattt gccgcgtgat
     3181 gatagtgtta tttagcatat tattcaaccg taaccgactt tgccaaattc ctgggctgat
     3241 cgacatcggt gcctttcagt acagccacgt aataagccag taactgcaag ggtaccgtgt
     3301 acacaatggg ccccaataat tcgggcacct ctgggatttc taacacgtga atgccaggcg
     3361 cttcctcgat gcctgaagat tttgttgtaa aaataagaag ctctccacct cttgcacgca
     3421 cttcttgaat attggatttt aatttttcca acaaaccatc atttgccgca acgacaacaa
     3481 caggcatttt tttatcgacc aatgctaatg caccatgttt taattcgcca gcaggatatg
     3541 cttctgcatg aatataagaa atttctttta attttaaggc gccttctatt acaataggat
     3601 attgcaagcc tctgcccaca aacagcgcgt gtgatttatt ctgaaataac gttgcaattg
     3661 tttggatagc cttcgcagaa gccaaacacc cttgtatgat atctggcagc tctaccaact
     3721 gttggatcaa caatatttct ttttcgcgct ctaatccgcg gcgacgcgcc aacacaaggg
     3781 tcaataaaaa taatgcaact aattgtgtgg taaaggcctt tgtcgaacac acaccaattt
     3841 ccggacctgc attcgttaaa aaaactaatg ccgattcacg tactaacgaa ctatccggca
     3901 cattacaaat acttaacacc ccgcaatagt tttcgccctg cgtgctgcgt aatgcggcta
     3961 aggtatctgc cgtttcgcca gattgtgaga tacatatcaa gagtgtattt ggcaataaga
     4021 cacgtttacg ataacgaaac tcgctagcaa tttcgacgtg acacgaaata cccgccaggt
     4081 tttccaacca taatcgacca atcataccgg catggtaact ggtaccaca
//
LOCUS       DAORVT010000072         4110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_16746_length_4110_cov_2.829100, whole genome shotgun sequence.
ACCESSION   DAORVT010000072 DAORVT010000000
VERSION     DAORVT010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16746_length_4110_cov_2.829100"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PK389_02145"
     CDS             <1..199
                     /locus_tag="PK389_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22545.1"
                     /translation="GETTCLSRKRYLVRGIETNKSELISAGSNALRKPSIASFEKPWR
                     VYLNQKWIDGKQILTPLKILS"
     gene            343..705
                     /locus_tag="PK389_02150"
     CDS             343..705
                     /locus_tag="PK389_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22546.1"
                     /translation="MTDRANESVIKTQRDRRKFSLDDKRRLCKVWQASGLSKHRFCEQ
                     NDLVLSALTRWCNKFLSGNVSENANWIPVETPDKPSSNTQAIEILLPNKAIVRVSLNS
                     SSLKTLLQELCHATTAIR"
     gene            complement(851..1282)
                     /gene="tnpA"
                     /locus_tag="PK389_02155"
                     /pseudo
     CDS             complement(851..1282)
                     /gene="tnpA"
                     /locus_tag="PK389_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007783294.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(1398..3266)
                     /locus_tag="PK389_02160"
     CDS             complement(1398..3266)
                     /locus_tag="PK389_02160"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQY22547.1"
                     /translation="MLDPIDTLGNIYSVAKLIYDQVQLVKANQQQCNRLVNRIRIIES
                     SVHRLDDITDKAQYQNGLKDLQSLLEESLSLITKFSTSSRWERFVLNTGNYKDDFQDL
                     NKRLKHSILQLTLGLVTKSTLDHETDRETDKADKIAQAADIAFIRENQRMILEANHKE
                     LEALQSIHLSQKDKEEVLHLQMQAIESQLKNIIQQKDTQSVHDNSRIPYYELVFEKLI
                     ETGSFGKIYLGKWEGRTVAIKYLEGKLTEAQSQEFIREETIMSGLRHPNVTAFYGACL
                     EDGHAGIVMEYMEQSSLEKVIAKKLTPTQQKSISLDIAKGLSYLHNNGVLHRDLKSAN
                     ILVNAQGTAKLADFGLSKTKDANVKTAQGRSKALAWQGPELFEYKAQYTPASDIYSFG
                     MILWEMLSGKKPFAELEDKKIPKAVQAGKRESLEGFPSMYVELITACWQAEPEKRPTI
                     KEIIAKLEAYEPEEKLDAEAYYRRGFGFEKAQKFSEAYLDYKSAAEIGHAKANTSAGL
                     LALAGKGVSSDKSEAHQRFLSAANQNHRRAMRCLAKMLQYGDGVSQDTAQALEWYEKA
                     NNLGEAKRKNKVLKPKAVLWLAPEVASISSSSSIVTATLTAPEKVQSGENLKKTGV"
     gene            complement(3478..>4110)
                     /locus_tag="PK389_02165"
     CDS             complement(3478..>4110)
                     /locus_tag="PK389_02165"
                     /inference="COORDINATES: protein motif:HMM:NF021897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HQY22548.1"
                     /translation="EDRDRRLWLFFDELASLEKLNSLAPVLSRGRGYGACFVGGIQDI
                     HQLRDIYGDKGAEVLISSFGTSLFFYQNNNRSAQWAAEQLGSAEFLENREGMSFGSHE
                     NRDGVNVNSFIKEQRLVLGSEIKNLRKGEAFMTTSGGWPVTKLAFNYQQREEKEPALI
                     PRNIDDISHQLIKNVDGSLEKLSVNNINPEVKKEVTQETKRTVEEAMELF"
BASE COUNT         1212 a          869 c          692 g         1337 t
ORIGIN      
        1 gggggaaact acatgcctct ccagaaagag atatctagta cgcggtatag aaactaataa
       61 atctgagctt atcagcgccg gttcaaatgc acttagaaaa ccatcaatag caagcttcga
      121 gaaaccttgg cgcgtttacc taaaccaaaa gtggatagac gggaagcaaa tactgacccc
      181 acttaaaatt ttatcttgac gcaacccttc aaatataata gatttctact ttcattttta
      241 gggtgctccc tcgtatccgg tcaacaatcc catgttgcag ggtctaatat aagtctttaa
      301 tctgtttata gcgtaaccat tatttatagt gaggaaacag ctatgacaga tagagccaac
      361 gagtccgtta taaaaacaca acgagatcga cgtaaattta gccttgatga taaacgaagg
      421 ctgtgcaaag tatggcaagc ttctggatta agtaagcata gattttgtga gcaaaatgat
      481 ctggtgcttt cagctttgac aagatggtgc aataaatttt tgtctggaaa cgtttctgaa
      541 aatgccaatt ggattccagt cgaaacacca gacaagccta gcagcaatac acaggctatt
      601 gaaattctat tgcctaataa ggcgatagtg cgtgtatcgt tgaattcctc ttcccttaaa
      661 acattgctgc aagagctgtg ccatgcaact acagctatac ggtaataaaa tttgggtatt
      721 caaaaatcct gttgatttta gtaaatctat agatgggtta agtaatctta ttgttgaaga
      781 tgtgaatcac taaccgcctg aaggcggtag cttcagtaaa cgcttgcaaa gcggattaac
      841 gcttaaaagc ttaatattca gaaatcgaaa agttatcttg cttatgctgt atctcttggt
      901 gctcaatgta ttcttgtact tgctgctcat ttaaatttcc aaccgttact gcaaaatatc
      961 cccttgccca aatatgttga ccccaatatc gttttttcaa atgttcaaat tccatcagta
     1021 tttttcggct actcttccct ttaatatatt ggagcaattt cgaaatcgaa atacttggag
     1081 gaactaatac caaaaggtga atgtgatcag gactcatact ccctgaaaca atatcgacgt
     1141 aatttgctaa gcatatttca cgaataagct atcggactcg gttagccact gccccggtca
     1201 aaacacgata cctatacttg gtacaccaaa ttaaatggta cttcaaatca taggtcgaat
     1261 gagatccttt acgatacgtc atcccctaag aatactaaag tgttcgccta aaggcgaggg
     1321 tgttattccc atcccacagg agagacataa aatttaactt gtacgtacag gttaggaaaa
     1381 attcattata catctaatta aacgcctgtt ttttttaaat tttcaccaga ttgaaccttt
     1441 tctggcgcag ttaacgtggc cgtaacaatt gaagatgatg atgaaataga agccacctca
     1501 ggtgctaacc ataaaacagc ttttggcttc aacactttgt tttttctttt ggcttctcct
     1561 aaattatttg ctttttcata ccactctaag gcttgagccg tatcttggga tactccatcc
     1621 ccatactgta acattttggc aagacatcgc atagcccgac gatggttttg attggcagca
     1681 cttaaaaaac gctgatgagc ttctgactta tcactcgata cccctttgcc cgctaaagca
     1741 agcaatccag cactggtatt ggctttagca tgcccaatct ccgccgcact tttgtaatca
     1801 aggtaagctt cagaaaattt ctgtgctttt tcaaacccaa aaccccttcg ataataagct
     1861 tcagcatcca gtttttcctc tggctcataa gcttcaagct ttgcaataat ttccttaatg
     1921 gttggacgtt tttcgggttc tgcttgccaa caagctgtaa tgagttctac atacatagat
     1981 ggaaaacctt ccagactctc tcttttacca gcttgcactg ctttagggat tttcttatct
     2041 tcaagttcag caaaaggctt tttacctgat aacatttccc agagtatcat accaaaactg
     2101 taaatgtccg atgctggcgt atactgcgct ttatattcaa ataattctgg accttgccaa
     2161 gcaagtgctt tactgcgtcc ttgagctgtt tttacgtttg catctttagt ttttgataaa
     2221 ccaaaatctg ctagtttcgc tgttccttgt gcattcacta aaatatttgc gctttttaag
     2281 tctcgatgta acacaccgtt attgtgcaag taggatagtc ctttggcaat atctaggctt
     2341 atagattttt gctgtgtggg tgttagtttc ttcgctataa ccttttccaa ggagctttgc
     2401 tccatatatt ccatgacaat accagcatgc ccatcttcta aacaagcacc ataaaatgct
     2461 gtaacattag gatgccttag accactcata atggtctctt ctcgaataaa ttcttgtgat
     2521 tgcgcttctg ttaactttcc ttctaaatac ttgatcgcga ccgtacggcc ttcccacttc
     2581 ccaagataaa tctttccaaa agaacctgtt tcaattaact tttcaaatac caattcatag
     2641 taaggaatac ggctattatc atgcactgat tgagtgtctt tttgttggat aatatttttt
     2701 aattgagact ctatagcttg catttgcagg tgcaacactt cttctttatc tttctgggat
     2761 aaatgaatgc tttgtaatgc ctcaagctct ttatgatttg cctctaaaat cattctttga
     2821 ttctctcgta taaacgctat atccgctgcc tgagctattt tgtctgcttt atcagtttca
     2881 cggtctgttt cgtgatctaa cgtacttttt gtaacaagac ctaaggttaa ttgtaaaatc
     2941 gaatgcttaa gtcttttatt taaatcttga aaatcatctt tataattgcc tgtatttaaa
     3001 acaaatcgtt cccaacgact actggtgcta aattttgtga taagagacaa agattcttcc
     3061 aataaagatt gtaaatcttt taagccattt tggtattgtg ccttgtctgt aatatcatct
     3121 aaacgatgaa ccgaagattc aataatgcgg atacgattta ccaagcgatt acactgttgt
     3181 tgatttgctt taactaattg aacctggtca taaattagct ttgcaaccga atatatattt
     3241 cctaaagtat ctattggatc taacataaca cctccatgat aagtgcttag ccttttaaaa
     3301 aagccaaata aaaaagaaaa aaatgcaatc atgataaatt acaatggaca gaatgccagc
     3361 tttaaaaaaa atagtcaaat ttattgattt ttattggtat tagccaattc tagctagtaa
     3421 gctagaaatc aagacaattt cctattaaag aagttagcat acgtttctgt ctatggttta
     3481 aaacaactcc attgcctctt ctactgttcg tttcgtttct tgagttacct cttttttaac
     3541 ttctggatta atgttgttaa cgcttaattt ttctaagctg ccatccacat ttttaatcaa
     3601 ctgatgacta atatcatcga tgtttctggg aattaaagca ggctcttttt cctcccgttg
     3661 ctgatagtta aatgctaatt ttgtgaccgg ccagccgccg gaagtggtca taaaggcttc
     3721 cccttttctt aagtttttaa tttcagagcc taaaactaaa cgctgctctt tgataaatga
     3781 attcacatta acgccgtctc gattttcatg cgacccgaaa gacatgccct ctcggttttc
     3841 taaaaactca gcactgccta attgctccgc tgcccattgt gcagaacggt tgttattttg
     3901 ataaaagaat aacgaagtcc caaaactcga gatcaatacc tcagcaccct tatcgccgta
     3961 aatatcccgc aattggtgaa tatcttgaat accacccaca aaacatgccc cataccctct
     4021 gcctcgagat aaaaccggcg ctaaactgtt gagcttttct aaactggcca attcatcaaa
     4081 gaacaaccat aaccttcggt ctctgtcttc
//
LOCUS       DAORVT010000073         4108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_16762_length_4108_cov_3.598322, whole genome shotgun sequence.
ACCESSION   DAORVT010000073 DAORVT010000000
VERSION     DAORVT010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16762_length_4108_cov_3.598322"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="PK389_02170"
     CDS             complement(<1..885)
                     /locus_tag="PK389_02170"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HQY22549.1"
                     /translation="MDETSQKVPLGGLARKLIQIGLLNEETACSITESSIKQRIPFVS
                     TLVEEHYIDPEVLGTTCSHEFGIPFLDLNAFDTQHIPTDLINQDLVVQYHALPIFRRG
                     NRLYVAISDPTNLKAVDEIRYQTNTNTEAILITEHKFNELIQKVLSTSSEKSLGNLED
                     VDLESIDILNGDDSEESSKTTLGTDAEDAPIVRFINKVILDAVNLGASDLHFEPYEKT
                     YRIRFRRDGILYENSTPPVNISNRFSARLKVMARLDISERRIPQDGRFKMRISNNRAI
                     DFRVSTCPTLFGEKIVMRV"
     gene            complement(1146..1781)
                     /locus_tag="PK389_02175"
     CDS             complement(1146..1781)
                     /locus_tag="PK389_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015486923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQY22550.1"
                     /translation="MIKVMVVDDHELIRSGIRALLSDLPNIEVIATAASGEEAILLAK
                     QQKPNVILMDVRMPGIGGIAATQKITTAHPDIKIIALTSCTEEPFPTNLLKAGAHGYL
                     TKGATVEKMTQAIQNVAVGKKFLDPEIAHKLALKNTSNTLPSPFEQLSIREFNIVLML
                     NNGLNHKAIAEKLFISTKTLNSYRYNIYEKLNIKTDVELILLANQTGLIEP"
     gene            complement(1968..2336)
                     /locus_tag="PK389_02180"
     CDS             complement(1968..2336)
                     /locus_tag="PK389_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014996311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HQY22551.1"
                     /translation="MTIAYGCVLLMIIFPYFFTVLAKISPLFNNHTPREYLEHTTGWR
                     KRAHWVQLNSFEITPAFATGVIIAHQLHATQSSIDTLAITFVLTRCLYAACYLSNKAS
                     FRSIIWLIGFGCIIGLFFIG"
     gene            2471..3199
                     /gene="kdsB"
                     /locus_tag="PK389_02185"
     CDS             2471..3199
                     /gene="kdsB"
                     /locus_tag="PK389_02185"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HQY22552.1"
                     /translation="MNILCVIPSRIGSTRAPRKALLPIQGKPMVQWVYENASRCKAIT
                     KVVVATDSEEIADVIRSVNGNVMMTDSALPTGSDRVAYVASHYPEMAVVINLQGDEPF
                     VKPRMLEQLLSPYLAGESPDMTTLAHSLYDETDFNNPGAVKVVTDLHGNALYFSRSPI
                     PYFREAVDTPIYHHIGMYAYRRDFLLNYTQLPQTPLEKTELLEQLRAIEHGYKIRVCI
                     TEDRTLEVNTLEEYALAQNFVYTE"
     gene            3399..>4108
                     /locus_tag="PK389_02190"
     CDS             3399..>4108
                     /locus_tag="PK389_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY22553.1"
                     /translation="MKPNFDSAHNIVQINDLRFSRNSKLIFENVSLSVQRGKITAVMG
                     PSGSGKTTLLRLIGGQLSPDAGTVIAFGENIPELSRDGLHKIRRHMGMLFQSGALFSD
                     ISVFENVAFPLREHTRLPESMIRDLVLMKLQAVGLRGARNLMPSELSGGMSRRVALAR
                     ALAMDPTLIMFDEPFAGQDPITMGVLVKLIRLLNDALKMTTIIVSHDVQETLSIADYI
                     YVIAEGRVVGEGTPEDLK"
BASE COUNT         1219 a          749 c          876 g         1264 t
ORIGIN      
        1 gactcgcatc acaatttttt cgccaaataa tgttgggcaa gtactaacgc gaaaatcgat
       61 agctcgattg ttagaaattc gcattttaaa tcgaccatct tgcggaatcc gtcgttcaga
      121 aatatctaaa cgtgccatga ctttaaggcg tgctgaaaaa cgatttgaaa tattgacggg
      181 tggtgtggaa ttttcataga gtataccatc gcgacgaaat cgaatacgat atgttttttc
      241 ataaggttca aaatgtaaat ccgaagcacc taaattgaca gcatccaaaa taactttatt
      301 gataaatcga acgataggcg catcttctgc atcggtgccc agggttgttt tagaagattc
      361 ttcactgtca tctccattta aaatatctat gctttctaga tcaacgtctt ctaaattgcc
      421 aagtgatttt tccgaggaag tggataacac tttttgaatt aattcattaa atttatgttc
      481 tgtaatcaga atggcttcag tgtttgtatt ggtttgatat ctaatttcat ccactgcttt
      541 aagatttgtt gggtccgaga tggcaacgta aagacgatta cctcgccgaa aaatgggtaa
      601 agcatggtat tgcacaacaa gatcttggtt aataagatca gtgggaatgt gttgagtatc
      661 aaatgcatta agatcaagaa aaggtatgcc aaactcgtga gagcacgttg tgccgagtac
      721 ttccggatcg atgtaatgct cttcgactaa tgtgctaaca aagggaattc tttgtttaat
      781 agacgattct gtaatgctac aggcggtttc ttcgtttagt agccctattt gaattaactt
      841 gcgagcaagt cctccgaggg ggactttttg acttgtttca tccattgata gcaatgacta
      901 ttatcaattc gattaacgaa aacccttttg ttctaaattt catagctagg cactcctgac
      961 gtgttcgatt atacttaatt acaatgcagg aattgtgcca agagaggttt tttaatagta
     1021 tcagtttgat tttattgaga aaatagagtg gaatcataag ggagactggt taaaaagcgt
     1081 catgtaatat acgatttaaa aaagagaggg tcagaaattt gactgtcttt tttagcgctg
     1141 cgtatttagg gttctataag acctgtttga tttgcaagta atattaattc aacatccgtt
     1201 ttgatattca atttttcgta aatattgtaa cgataactat tgagtgtttt agtgctgata
     1261 aacaattttt ctgcaatggc tttatggtta agtccgttgt ttagcatcag tacaatatta
     1321 aattcacgaa tagataattg ttcaaaaggc gatggaagtg tgttggaggt attttttaaa
     1381 gcaagtttat gggctatttc cggatctaag aattttttac ctaccgcaac attctgaata
     1441 gcctgtgtca ttttttctac agtggcaccc ttggttaaat atccatgtgc gcctgctttt
     1501 aaaagattag tcgggaaagg ctcttcggta caagaagtta aagcgataat tttaatatca
     1561 ggatgagccg tcgttatttt ttgggttgca gcaatgccac cgataccagg cattcggaca
     1621 tccatgagaa taacattcgg cttttgttgt ttggctaata ggatagcttc ttcgccgcta
     1681 gcggcagtag caatgacttc aatgtttggc agatcggaaa gcaatgctct aatcccactg
     1741 cgtattaatt catgatcatc aactaccatt acttttatca tttttctcat cctcaatttt
     1801 tcaattatat gctagcgttc caacaacaaa cgagtttaat aattttgtgc aaaatcatat
     1861 agtaaggtca gtttgatctt gacataaaaa tataatctgc acaacttgct tcatgagcaa
     1921 ggaatgttac agcgtagagg tcgatcgctg tctcgatcct aaaaaaatca gcctatgaaa
     1981 aataagccaa tgatgcaccc aaacccgatg agccatataa tgctcctaaa gctagcttta
     2041 ttgctcaggt agcaagcagc gtaaagacat cttgttagga caaaagtaat ggctaaggtg
     2101 tcgatggatg attgagtggc gtgcaattga tgagcaataa taacgccagt tgcaaaagcg
     2161 ggtgttatct caaaactatt gagctgtacc cagtgtgcac gtttgcgcca tccggttgtg
     2221 tgttctagat attcgcgtgg cgtatgattg ttaaaaagag gactgatctt cgcgagaaca
     2281 gtaaaaaaat aaggaaaaat aatcattaag agtacgcatc cgtaggcaat ggtcatattt
     2341 ataatcccta aaaatagatt cattatccga gcttgataga ggttgtcaaa cgtcagacaa
     2401 ataacttttt tataaaaagc gctttaaaaa cgtattgaaa gcattcatga gatctcccat
     2461 aatcagctct atgaatatcc tctgtgtaat tcctagtcgc attggttcga cacgcgcgcc
     2521 gcgaaaggcg ttattgccta ttcagggtaa gccgatggtg caatgggttt atgaaaacgc
     2581 gagtcgctgt aaagccatta caaaagtggt tgtcgcaact gatagtgaag agattgccga
     2641 tgttattcgt tccgttaatg gcaatgtgat gatgacagac tcggcgttgc caacggggag
     2701 cgatcgtgtt gcgtatgtgg ctagtcatta ccctgaaatg gcagtcgtga ttaatttgca
     2761 aggcgatgag ccttttgtaa agcccagaat gctagaacag ttgctcagtc cgtatctggc
     2821 gggtgaatcc cctgacatga cgactttggc gcattctttg tatgatgaaa cagattttaa
     2881 taatccgggt gctgtcaaag ttgtaacgga tctacacggt aatgcgcttt atttttcgcg
     2941 ttcccccatc ccttatttta gagaagccgt agatacgccg atttaccatc atattggcat
     3001 gtatgcttat cgtcgtgatt ttctcttaaa ctatacccaa ttaccgcaaa ccccgcttga
     3061 aaaaacggaa ttgttagaac aattacgtgc cattgagcat gggtataaaa tacgggtttg
     3121 tataacagaa gaccgaacat tggaagtcaa tacacttgaa gagtacgcgc tagcacaaaa
     3181 ttttgtctac accgaataaa taaatcaagt cgaaaactct tctagtctcg cataagtgaa
     3241 atcactttat tgcatttaaa atcaatatgt tacaatttcc gcttgtagcc gaccgatatc
     3301 agaaaaaatg taagcgttaa cacatgcgta gacaaagcag cagcggctca agtatgctac
     3361 ggcattctta aatcagatcc ttatgatatc aaaaagttat gaagccaaat tttgatagtg
     3421 ctcacaatat tgtccaaata aacgacttac gctttagtcg taattctaaa cttatttttg
     3481 aaaacgttag cctctcagtg cagcgtggta aaatcactgc ggtgatgggt cccagcggtt
     3541 caggtaaaac gacgttatta agattaattg gtgggcaatt aagtcccgac gctggcacag
     3601 tcatagcttt tggtgaaaac attcccgaac tttcgcgtga tgggctgcac aagattcggc
     3661 gacatatggg tatgttgttt caaagcggcg cattattttc ggatatctct gtttttgaaa
     3721 atgttgcttt tcctttaaga gagcacacgc ggctgccaga atccatgatt cgagaccttg
     3781 ttttaatgaa actgcaagcg gtggggttaa gaggtgccag aaacttaatg ccttcagaat
     3841 tatccggtgg aatgagcaga cgcgttgcct tggcaagagc gcttgcaatg gatccgacct
     3901 taattatgtt tgacgagcct tttgcggggc aagatcccat taccatgggg gtattggtta
     3961 agttgattcg tctgttaaac gatgctttaa aaatgactac cattattgtt tcccatgatg
     4021 ttcaggaaac actcagtatt gccgattata tttacgttat tgcagagggt cgcgtggttg
     4081 gagaaggcac tcctgaagat ttaaagca
//
LOCUS       DAORVT010000074         4096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_16858_length_4096_cov_3.439000, whole genome shotgun sequence.
ACCESSION   DAORVT010000074 DAORVT010000000
VERSION     DAORVT010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16858_length_4096_cov_3.439000"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PK389_02195"
     CDS             <1..534
                     /locus_tag="PK389_02195"
                     /inference="COORDINATES: protein motif:HMM:NF020940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HQY22554.1"
                     /translation="LDQKAKQSILELLREINSQLKLTILLITHEIEVIKSICNRLAVL
                     HKGEIVEQNTVIEWYSKPKTDASKAFIRAETQLDIPASLGNRLLTEPCENTNTILRLS
                     FSKSAAEEPFIALVIQQFKLSVNIIQAHLENFQIHSVGIMIVEMVGERTEVHQAITFL
                     EHKDIHIEVIGYVARTA"
     gene            515..1171
                     /locus_tag="PK389_02200"
     CDS             515..1171
                     /locus_tag="PK389_02200"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HQY22555.1"
                     /translation="MLHELLNALEETLFMVFSAGLLTWVMGLPIGALLSVTNTGKILE
                     NKLIYKTLHFCIHTMRSLPYIVFMIAMIPFTQFLVGAYEGCIAAVVPLTLAAIPYFAQ
                     LSEKALNRIQPGIIEAAKAVGASPFQIIYKVLIPEALPNIIKALTLTLVHLIGYSTIA
                     GAIGAGGLGSLMIYKGYHAFHFDYVFATAVILIALIHIIQFSGHYIASGNMNRNSTAL
                     "
     gene            1204..2496
                     /gene="nhaD"
                     /locus_tag="PK389_02205"
     CDS             1204..2496
                     /gene="nhaD"
                     /locus_tag="PK389_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020026979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HQY22556.1"
                     /translation="MKQTLLLNVTHSIPALIALFTFFIAYLLVIFEEFTHLRKSKPLI
                     FASGIIWVMVSILANQKKVPEIAHMAIQHNLMQYAEILLFIIVAITYINVLTERNVFE
                     ALRAWLTKFKLSYRQLFWITGSITFLLSPCVDNLTSALAMSAVILALSKDNRRFAGLA
                     CINVVVAANAGGAFSPFGDITTLMVWQSGLIPFADFFKLFLPALISFLIPALCMHFAV
                     PIATPKSIDETVKITQGGKRVILLFILTIATTVYFQNNFHLPPVIGMMMGLAYLQLFA
                     YYTQQVKSLKTLEIFPAIKELDWDTLLFFYGIMLSIGGLATLGYLEIVSTTIYANHTS
                     GNILIGILSAIVDNIPLMFAVLTMNPSMSEGQWLLLTLTTGIGGSLLSIGSAAGISVM
                     GQARGIYTFFTHLKWIWAIALGYAASIACHLWLNAAYF"
     gene            complement(2550..3272)
                     /gene="dnaQ"
                     /locus_tag="PK389_02210"
     CDS             complement(2550..3272)
                     /gene="dnaQ"
                     /locus_tag="PK389_02210"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005493666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HQY22557.1"
                     /translation="MQKIKRQVILDTETTGLDPSKGHRLTEIGCLEMIDRRLTGRKFH
                     TYLNPQREVEKTAAAITGLTTEFLRDKPKFSYAVAEELVAFLKEGDTELIIHNAPFDL
                     GFLNYELMRVNHPWQPIEDYVGVIDTLVMARRMHPGQRNNLDVLCARYKVDNTVRQYH
                     GALLDAQLLAKVYLAMTAGQTKMQLGTESTVTEVAQPKLSIQRVKRNLDKVLRVIRAT
                     EEESRLHEARMAFLKEASGTKS"
     gene            complement(3289..3741)
                     /gene="rnhA"
                     /locus_tag="PK389_02215"
     CDS             complement(3289..3741)
                     /gene="rnhA"
                     /locus_tag="PK389_02215"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006788622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HQY22558.1"
                     /translation="MSNKIELYVDGACSGNPGPGGWGVLLRYQGIEKELSGAELNSTN
                     NQMELMAAIKGLQALTRPCHVDVYTDSEYVKKGITLWIHNWQKRGWKNSANKPVKNQA
                     LWEQLDAATHRHQITWHWVKGHSDHPENDRVDALAREAIDRLLSSEVK"
     gene            complement(3818..>4096)
                     /locus_tag="PK389_02220"
     CDS             complement(3818..>4096)
                     /locus_tag="PK389_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22559.1"
                     /translation="ESKNDNALVMLLGVLDNLYSLNSTQDIHQKLEPAESFFQFLKAR
                     EREKSIAHPSVLTLSGWPPRHKAQAAAGAKPKPEDPRQKKSGLPPPSP"
BASE COUNT         1118 a          920 c          770 g         1288 t
ORIGIN      
        1 ctcgatcaaa aagcaaaaca atctatttta gaattgctga gagaaatcaa tagccaacta
       61 aaattaacca ttttattgat tacccatgaa attgaagtga ttaaatccat ttgcaatcgc
      121 ctagctgtat tgcataaagg tgaaatcgtc gaacaaaaca cagtaattga atggtatagc
      181 aagcccaaaa cagatgcgtc taaagccttt attcgagcgg aaacacaatt agatatcccc
      241 gccagcttag gcaatcgtct actcaccgag ccctgtgaaa atacaaatac tattttacgt
      301 ctctcttttt ctaaatctgc tgcagaagaa ccttttattg ccttagtcat tcaacaattt
      361 aagttgtcgg taaacattat acaggcacat ttagaaaact ttcaaattca ttctgttggc
      421 atcatgattg ttgaaatggt cggcgagcgt acggaagtac atcaagcgat tacatttttg
      481 gaacacaaag acattcacat cgaggtaata ggctatgttg cacgaactgc ttaatgcctt
      541 agaagagaca ctgtttatgg ttttttctgc cggattactc acctgggtta tgggtttacc
      601 catcggtgcg ctcttatctg tcaccaatac cggaaaaatt ttagaaaata agctgattta
      661 taagacgctg catttttgca ttcatacgat gcgatcgcta ccttacattg tctttatgat
      721 tgccatgatt ccgtttacac agtttctcgt tggcgcttac gaaggctgca ttgctgcggt
      781 agtcccctta acgctagcag cgatccctta ttttgctcaa ctctccgaaa aagcactaaa
      841 ccgtattcaa cctggcatta tagaggccgc taaagccgtc ggcgcctcgc cttttcaaat
      901 tatttataaa gtattgattc cagaagcatt acccaatatc ataaaggcac ttacgctgac
      961 gttggtccat ttaatcggat attcaaccat cgcgggggcc attggcgcag ggggacttgg
     1021 gagcttaatg atttataaag gatatcacgc ttttcatttt gactatgtct tcgcaaccgc
     1081 tgttatctta attgcgctta ttcatattat ccaattcagc ggacattata ttgcgagtgg
     1141 caacatgaac cgtaatagta cagcgcttta acggtaaaat acaataaaac acggtataaa
     1201 acaatgaaac aaactttgct cttaaatgtc acgcattcta tacctgcact gattgcgctc
     1261 tttacatttt tcatcgccta tctactagta attttcgagg aatttactca tctacgaaaa
     1321 tcaaagccac tcatttttgc ctcgggtatt atttgggtaa tggtctcaat actcgccaac
     1381 caaaaaaaag taccagaaat cgcacatatg gctattcagc ataatctgat gcaatatgca
     1441 gaaattttat tatttattat tgtcgctatt acctatatta atgtcttaac cgaacgaaat
     1501 gtttttgaag cattacgtgc ttggcttaca aagtttaaac tgtcttaccg tcaactattt
     1561 tggataaccg gcagcataac ctttttatta tcgccatgcg tagataattt aacttcagca
     1621 ctcgcaatgt ctgccgttat tttagcatta agcaaggata atcgacgttt tgcagggcta
     1681 gcttgtatta atgtcgtcgt tgcagcaaac gcaggcggcg cttttagccc ttttggggat
     1741 attacaactt taatggtctg gcaatcgggt ttaattccat tcgcagattt ttttaaatta
     1801 tttttgcccg ccttaatcag ttttttaatc cccgcactgt gcatgcattt tgcagtaccc
     1861 atcgccacgc ccaagtcgat cgacgagacc gttaaaataa cccaaggtgg caaacgcgtt
     1921 attcttttat ttattttaac gattgccaca acggtttatt ttcaaaataa ttttcacctg
     1981 ccgccagtta ttggcatgat gatgggtctt gcttatctac aactttttgc atattacaca
     2041 caacaagtaa aaagtctcaa aacactggaa atctttcccg ccattaaaga attggattgg
     2101 gatacgcttt tatttttcta cggcattatg ctctcaatcg gcggcttggc aacattaggt
     2161 tatcttgaaa tcgtctctac aacgatttat gcgaatcata cttcaggcaa tatcttgatt
     2221 gggatattat ccgccattgt tgataatatt ccgctgatgt ttgccgtact cacgatgaac
     2281 ccatcgatgt cggaaggcca gtggctattg ttaaccctta caaccggcat tggcggcagt
     2341 ttgctgtcta taggctctgc cgcaggcatt agcgtgatgg gacaagctcg cgggatctat
     2401 acttttttca cgcatcttaa gtggatctgg gcgatcgctt taggctatgc ggcgagtatt
     2461 gcgtgtcatt tgtggttaaa tgcggcttat ttttgacggg cacctttttt gagggaatgt
     2521 tgttagcaaa aaagaaatta ttagtttaat taacttttgg ttcctgaagc ctctttcaaa
     2581 aacgccattc ttgcttcatg caatcgagat tcttcctcgg ttgctctaat cacccgcaac
     2641 actttatcca gatttctttt aacgcgctga atactcagct tcggctgtgc gacttccgtc
     2701 actgttgatt ccgtacccaa ctgcattttt gtttgcccag ccgtcatcgc caaatagact
     2761 ttggccagca attgcgcatc caacaatgca ccatggtatt gacgcacagt attatctact
     2821 ttatagcgtg cacaaagcac atccaaatta ttgcgttgcc caggatgcat ccgtctggcc
     2881 atgactaatg tatcaatcac acccacataa tcttcaatag gctgccacgg atgatttacc
     2941 cgcattaact cataattcaa aaatcctaaa tcaaaaggcg cattatgaat aatcagctcg
     3001 gtgtcaccct ctttaagaaa agcaacaagc tcttcagcca ccgcatatga aaattttggt
     3061 ttatcacgca aaaattccgt tgttaaacct gtaatggcag cagctgtctt ttcgacttca
     3121 cgctgtggat ttaaataggt atgaaatttt cgacctgtta agcggcgatc aatcatttct
     3181 aaacaaccaa tttccgttag acgatgtccc ttgcttggat cgagtccagt ggtttcggta
     3241 tctaaaataa cttgccgttt tattttttgc acaaacaaga tcctttcatc atttcacttc
     3301 agaacttaat aacctatcaa tcgcttctcg agcgagcgca tcaacgcgat cattttcagg
     3361 atgatccgaa tgccccttca cccaatgcca ggtgatttga tgccggtggg tggctgcatc
     3421 taattgctcc cataacgctt ggtttttaac cggcttgttg gctgagtttt tccacccacg
     3481 cttctgccaa ttgtggatcc aaagtgtaat acccttttta acgtactcag aatcggtata
     3541 tacatccaca tgacagggtc gcgtgagcgc ctgcaagcct ttaatagccg ccattaattc
     3601 catttggtta ttggtagagt taagttctgc acctgacaac tctttttcaa taccctgata
     3661 ccgcagcaaa acgccccacc caccgggtcc cgggttcccg ctgcatgcac catcaacata
     3721 gagttctatt ttattggaca cctaaattct ccatgtgctc atcatacaat gcttagtgta
     3781 ttgcgtcatc agcgccacaa aagaattttt tatttaatta cggcgagggg ggaggaagtc
     3841 ctgatttctt ttgcctcgga tcctcgggtt tgggtttagc cccagctgct gcctgagcct
     3901 tatggcgtgg tggccatcca ctcaatgtca atactgaagg atgcgctatt gatttttcac
     3961 gttctctggc tttcaaaaac tgaaaaaagc tttcggcagg ttcaagtttt tgatgtatat
     4021 cttgagtgct atttaaagaa tagagattat ctaaaacgcc taatagcatc acaagtgcat
     4081 tatcattttt ggattc
//
LOCUS       DAORVT010000075         4086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_16940_length_4086_cov_3.594890, whole genome shotgun sequence.
ACCESSION   DAORVT010000075 DAORVT010000000
VERSION     DAORVT010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16940_length_4086_cov_3.594890"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="PK389_02225"
     CDS             complement(<1..703)
                     /locus_tag="PK389_02225"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HQY22560.1"
                     /translation="MYSHNLKIAAFDPEIWTAISGEQRREEDHIELIASENFVSPCVL
                     EAQGSVFTNKYAEGYPRKRYYGGCQYVDIAEELAIARAKELFGAAYVNVQPHSGSQAN
                     AVVFMALLNPGDTILGMSLAHGGHLTHGASVNFSGKMYHAVQYGTNVNTGLIDYDEVE
                     KLALEHRPKLIVAGFSAYSRIVDWARFREIADKVGAYLMVDMAHVAGLVAAGLYPSPV
                     NIADVTTSNTHKTLRG"
     gene            complement(841..1278)
                     /locus_tag="PK389_02230"
     CDS             complement(841..1278)
                     /locus_tag="PK389_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22561.1"
                     /translation="MIERLSSIIHGEKFREQMIRFLPMDVQERTLLNKNYLYLLLQEI
                     GLILETLGLEWKEKNGDIYCTNFLRKLSVTHFPEVLIQMLKVRDKYGIVVAESQELIV
                     SSENKLIAKELSTGFNSFLPKGYKVDDSLQAGEWYIKFTKRIG"
     gene            complement(1301..1456)
                     /locus_tag="PK389_02235"
     CDS             complement(1301..1456)
                     /locus_tag="PK389_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22562.1"
                     /translation="MKIENNKFLNLVENAMREPGRMHMRPVIEKELLHYDILFALKEK
                     NTGLQNW"
     gene            complement(1446..1985)
                     /locus_tag="PK389_02240"
     CDS             complement(1446..1985)
                     /locus_tag="PK389_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22563.1"
                     /translation="MKKLQAIKTLRVWDKAGKYVFTKHQLCKLFPQDLPKTFNEGLKR
                     LVKDNILVRACRGIYINPQATSLDSYTIEHIAKALRPGEYNYVSLESILSEYGAISQI
                     PLDRITVVTTGRKGVYQTPYGTIEYTHTKRDWSDILKGIVTVEKRPLRIASKAMAWRD
                     LKRVGRNINMVNLEALNED"
     gene            complement(2290..3039)
                     /gene="rlmB"
                     /locus_tag="PK389_02245"
     CDS             complement(2290..3039)
                     /gene="rlmB"
                     /locus_tag="PK389_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005331757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HQY22564.1"
                     /translation="MREDLIYGIHAIQHLLNSKPEQFLELHIQDTHDNKSLLSLASLA
                     KTLGVNVSGVSKKQLDQWLPERNHQGVAAKVRLQPLLTEDDFSTLIDACDKPPLFLIL
                     DGVQDPHNLGACLRTADAVGVTAVIIPKDRSASLTPVVRKVASGAAETVPVVQVTNLV
                     RAIEMLKKLGVWIMGTSMQTTQSLYQVDLKGSVAIVLGAEGTGLRRLTEEHCDVLMQI
                     PMMGVVESLNVSVAAGVCLYEATRQRLNKVD"
     gene            complement(3040..>4086)
                     /locus_tag="PK389_02250"
     CDS             complement(3040..>4086)
                     /locus_tag="PK389_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HQY22565.1"
                     /translation="TVETRIIFAKDGKINKIEPVQRNDAHRLIEECMLSANVACARFL
                     KKHKLPGMYRNHEGPPEEKLADLKVFLNELGLPLSGGQKPQPLDYAALLRSIQKRPDA
                     NIIQMVLLRSLSQAVYSKDNLGHFGLAYPAYCHFTSPIRRYPDLLVHRQIRTVLRGQW
                     TEKKIEEANTDAFQESLVKLGDHSSMTERRADDATRDAVRWLKCQYIQKHLGEEFEGI
                     ISGVTRFGFFVELKDIYIDGLVHVTSLRNDYYYFDPIHHRLVGERSGIEYKLGGSVKV
                     KVIRVDIDDRKIDFELLESEATGKKQKRSRSEKGGNHKPGVEKNSKNKKNKSKNKSKS
                     RSHKNKKITKKRAD"
BASE COUNT         1207 a          918 c          680 g         1281 t
ORIGIN      
        1 gtcctctcaa tgtcttatgt gtgtttgagg tcgtcacatc cgcaatattc acaggagaag
       61 gatataaacc agcagctaca agacctgcga catgcgccat atcgaccatt aaataagcac
      121 cgactttatc agcaatctct ctaaatcgcg cccaatcgac aatgcgcgaa taggccgaga
      181 agcccgcaac aatgagtttg gggcggtgtt ctagtgccaa tttttcgact tcgtcataat
      241 cgatgagacc tgtattcaca ttcgtgccat attgcacggc gtgatacatc tttcctgaaa
      301 aattaacaga agcgccatgc gtaagatgac caccatgcgc aaggctcatc cccaaaatag
      361 tatcgcctgg attaagcaag gccataaaga caacggcgtt ggcctgcgag cctgagtgtg
      421 gctgcacgtt aacataggca gcaccaaata attcttttgc ccgtgcaatg gccaattctt
      481 cggcaatatc gacatactga cacccaccat aataacgttt gcgcggatat ccttcggcat
      541 atttgttggt aaacacagag ccttgggctt ctaaaacgca agggctgaca aagttttcgg
      601 aggcgatcaa ttcaatatga tcttcttcac gacgttgctc gccactgatc gcggtccaga
      661 tttctgggtc aaatgctgca atttttagat tgtggctata cacatgtact ctcctttgcc
      721 aaaagtcaaa gtccgcctaa ttcactattc tacctaagtt tttggatcta gcatagtcaa
      781 tacctgactt aattcagaca aagtttgaaa taaatcacat tggaatgaga atccagcatt
      841 ttatccaatc ctcttggtaa attttatata ccattcccct gcttgcaatg aatcatcaac
      901 cttgtagcct ttgggcaaaa atgagttgaa tcccgtggac aattcttttg ctattaattt
      961 gttttcagaa gaaacaatta attcttgtga ttcagcaaca acaatgccat acttatctct
     1021 aacttttagc atctgaatca aaacctctgg aaagtgggtg acactcaact ttcttaggaa
     1081 atttgtacaa taaatatctc catttttttc tttccattcc aaacctaacg tttctagtat
     1141 taaacctatt tcttgcaata ataaatatag ataattttta tttaataatg ttctttcttg
     1201 tacatccata ggtaagaagc gaatcatttg ttctctaaac ttttccccat gaataatgct
     1261 cgataaacgt tcaatcattt tgtcaagctt ttgtggataa tcaccaattt tgtaatcctg
     1321 tatttttttc tttaagtgca aatagaatat cgtaatgcaa aagttctttt tctatcacgg
     1381 gtctcatgtg catacgacca ggttcacgca tagcgttttc taccaaattt agaaatttat
     1441 tattttcaat cttcattcag tgcctctaaa ttaaccatat taatgttacg gccaacacgt
     1501 tttaaatcac gccaagccat tgctttagaa gcaatgcgca ggggtctttt ttccactgtt
     1561 acgatccctt ttaaaatatc agaccaatct cgtttagtat gtgtgtattc aatagtgcca
     1621 tatggcgttt gataaacacc ttttcgccca gtagtaacaa ctgttatcct atccagagga
     1681 atctgagaaa ttgcaccata ctcagataaa atggactcta gactgacata attgtattca
     1741 ccaggacgca aagcttttgc aatatgttcg atggtataac tatccaaaga ggtcgcttga
     1801 ggattaatgt aaattccacg acaagctctt actagaatgt tatctttaac caatcgttta
     1861 agcccttcat taaaagtttt aggcagatct tgtggaaaaa gtttacaaag ctgatgcttt
     1921 gtaaatacgt attttcccgc cttatcccat acccttaaag tctttatggc ttgaagtttc
     1981 ttcatatttc ccctttaagt ggacattaat accacaaaaa tttagctttt ttgtctactt
     2041 aatagaatag cttattaata agtagacgtc aaaactatat tttaatagta tttctgttca
     2101 cttaaaaaac cttctctctc ctgtaaaatg tctgtaaata caacaaaaaa cactaaatat
     2161 catatttagt taaagtatat ttgcactaat tggctgtttt tacgcattaa agagaaagtt
     2221 aaacgactta aaatgggttt aatactaaag cgtctcagtt aaaattacca aaaaagagat
     2281 agtcataaat taatcaactt tgttcagtcg ctgccgggtt gcttcataca agcaaactcc
     2341 cgcagcgacc gagacattta agctctcgac cacgcccatc atgggaattt gcattaagac
     2401 atcgcagtgc tcttcggtta aacgccgaag acctgtgcct tctgcaccga gcacaatcgc
     2461 aacagaacct tttaaatcca cttgatacaa agattgggtt gtttgcatgg atgtacccat
     2521 gatccaaaca ccgagttttt tgagcatttc tatcgcgcga accaaattgg tgacttgcac
     2581 caccggaact gtttcggcag caccactcgc aaccttacgc acaacgggcg ttaagctcgc
     2641 agagcgatct ttgggtataa taacggctgt cacacctaca gcatctgctg tgcgtaaaca
     2701 agcccccaaa ttatgcggat cttgtacacc atctaaaatt aaaaacagcg gcggtttgtc
     2761 acaagcatct atcaacgttg aaaaatcatc ttccgttaat aacggctgca aacgcacttt
     2821 agcagcgaca ccctgatgat tccgttctgg caaccattga tctaattgtt ttttagaaac
     2881 gccacttaca ttcacaccca atgtttttgc cagcgacgcc agcgataaaa gcgatttgtt
     2941 atcgtgtgta tcttggatat gcagttctaa aaactgctcg ggttttgaat tcaacaaatg
     3001 ctgtatcgca tggatcccat aaattaaatc ttcacgcatt taatctgctc tttttttggt
     3061 aattttttta tttttatgac ttctactttt gcttttattt ttgcttttat tttttttgtt
     3121 tttactgttt ttttcaacgc ctggtttgtg attgccgcct ttttcggacc ttgagcgctt
     3181 ctgtttttta cccgtcgctt ctgactcaag caattcaaaa tcgattttac gatcatcaat
     3241 atcaactctt atcaccttaa ctttaacgct accgcccagt ttatattcaa tacccgaacg
     3301 ttcgccgacc aagcgatgat gaatcggatc aaagtaataa taatcattac gtaggcttgt
     3361 aacatgaacc aaaccatcaa tataaatatc ttttaattct acaaaaaatc caaaacgtgt
     3421 cacacccgag ataattccct caaattcttc acccaggtgc ttttgaatat actgacattt
     3481 taaccaacga actgcatctc gtgttgcatc atcagcacgc ctttctgtca tagaactatg
     3541 atcgcctaac ttgaccaaag attcttgaaa tgcgtctgta ttcgcttcct caatcttttt
     3601 ctctgtccat tgcccacgca acaccgttcg aatttgacga tgcaccaata gatcgggata
     3661 acgccgaatg ggagacgtaa aatgacaata agctggatac gccaacccaa aatgccccag
     3721 attatcttta gaataaaccg cctggcttaa agaacgcaat aacaccattt gaataatgtt
     3781 tgcatctggc cgcttttgaa tagaacgtaa caatgcagca taatccaagg gctgtggttt
     3841 ttgcccccca ctcaaaggta accctaattc attgagaaac acttttaaat cagctaattt
     3901 ttcttccggt gggccttcgt gattacgata catccccggc aacttatgct ttttcaaaaa
     3961 gcgagcacaa gccacattag cacttaacat acattcttcg attaagcgat gtgcatcatt
     4021 acgctgaacc ggctcaatct tattgatttt gccatcttta gcaaaaataa tacgggtttc
     4081 taccgt
//
LOCUS       DAORVT010000076         4070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_17062_length_4070_cov_3.797509, whole genome shotgun sequence.
ACCESSION   DAORVT010000076 DAORVT010000000
VERSION     DAORVT010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17062_length_4070_cov_3.797509"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /gene="leuS"
                     /locus_tag="PK389_02255"
     CDS             complement(<1..1010)
                     /gene="leuS"
                     /locus_tag="PK389_02255"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011234198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HQY22566.1"
                     /translation="MESQYNPQKIEAEAQAYWQKSQTFKASEDPLREKFYCLSMFPYP
                     SGSLHMGHVRNYTIGDVISRFQRMQGKNVLQPIGWDAFGLPAENAAIKHKVPPAEWTY
                     QNIELMREQFKRLGFGYDWDRELTTCKPDYYRWEQWFFVKLFEKGLVYKKNSVVNWDP
                     VDQTVLANEQVIDGCGWRSGAKVERREIPQWFLKITAYAEELLKDLDTLTGWPEPVKT
                     MQRNWIGKAIGAAVQFKVEERSDVIEIFTTCPDTLFGVTYLAIAPEHPLSLAVAENNS
                     EIHQFIEACRIGKVAEAEFATLEKCGLDTGLKAINPVNGEAVPVWVANYVLMEYGTGA
                     IMA"
     gene            complement(1028..2536)
                     /gene="lnt"
                     /locus_tag="PK389_02260"
     CDS             complement(1028..2536)
                     /gene="lnt"
                     /locus_tag="PK389_02260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HQY22567.1"
                     /translation="MLNLTLNKYQLLIIAMLAGAILPGAFAPHHLWPLAIVSPAILLW
                     VWQKNTTSKQAFMSGLGYGLGMFGVGVSWVFVSIHHYGNTNIPLAVLITSILVIVLAL
                     FIATQGYVLKHFFRGNTVAFCLLGFPSCWVLFEWLRSWLFTGFPWLYLGYSQLTTPLS
                     GYAPVMSVYGVSTAVLLTSGALIVLMISSRKIKAAGSFLIILIWGIGYFLQQHNFTKP
                     NPKLYTVRLVQGNIKPFDKFTSTDPIGSTEKTYGKLTEKEWGADLILWPESAIPLPLP
                     YSKPYFDELEKRAFSHHSTLIAGIQVINSKEEYHNSLIALGDGHGIYHKYHLVPFGDF
                     LPFDYWLRGLIGFFDLPMSSFTSGPTEQPLITAGDLKLVPLICYEIAFPELLRNTLRN
                     ADAIITLSEDGWFGESWGPYQHLEIAQMRALETGRMVLRATTSGITAIIDPKGTLSAT
                     IPQFEARVLSGTFHSMTGTTPWVKFGLWPLLGLLFSCFILPGRLLCCRHRIS"
     gene            2879..3142
                     /locus_tag="PK389_02265"
     CDS             2879..3142
                     /locus_tag="PK389_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22568.1"
                     /translation="MCLKKDDKAAGEGTGVERESYRQGDLNPIDKLQFAKWVLKGIFV
                     FFVISIAVRLYQEGSGETLMDICRTGLLPIASFVIGDYFGSRG"
     gene            3142..4047
                     /gene="rimK"
                     /locus_tag="PK389_02270"
     CDS             3142..4047
                     /gene="rimK"
                     /locus_tag="PK389_02270"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HQY22569.1"
                     /translation="MKIAILSRRARLYSTQRLVEAAKIRGHEVEVIDTLRCYMNIASY
                     KPSIHYKGRKLEGFDAVIPRIGASITFYGTAVLRQCEMMGIFPLNESVAISRSRDKLR
                     SLQLLARKGIGLPVTGFAHSPDETQDLIHLVGGSPLVIKLLEGTQGMGVVLAETPKAA
                     ESVIDAFAGLKVNILVQEFIKEAECSDIRCFVVGSKVVATMKRQAKPGEFRSNLHRGG
                     TSSTVKITPEERSTAVRAAKIMGLNVAGVDILRSNNGPVVLEVNSSPGLRGIETTTGL
                     DVASIIIDFIEQNAKPNSTGTKGKG"
BASE COUNT         1198 a          802 c          914 g         1156 t
ORIGIN      
        1 gccataatcg cgccggttcc gtattccatc agaacataat tggcgaccca gacgggaacg
       61 gcttctccgt taaccgggtt tatggcctta agcccagtgt ctagaccgca tttttcaagc
      121 gttgcaaatt ctgcttctgc gacttttcca atgcgacagg cttctataaa ttgatggatt
      181 tccgaattgt tttctgcaac tgcaagtgag agtggatgtt caggtgcaat ggcaagataa
      241 gtaacgccaa ataaagtgtc ggggcatgtg gtaaaaattt caataacgtc tgatcgttct
      301 tcgactttaa attgtacagc agcgccgatt gctttaccta tccagtttcg ttgcatagtc
      361 ttaaccggtt ctggccaacc cgttagtgtg tctaaatctt ttaagagttc ttcagcgtag
      421 gcggtaattt ttaaaaacca ttgcgggatc tcgcgacgct caactttagc gccagaacgc
      481 caaccacaac catcgatgac ttgttcattt gccaaaacgg tttgatcaac gggatcccaa
      541 ttcacaacag aatttttttt gtagaccaat cctttttcaa aaagcttaac aaaaaaccat
      601 tgctcccaac gatagtaatc cggtttacat gtagtcaatt cgcgatccca atcatatccg
      661 aaccctaagc gtttaaattg ttctcgcatc agttcgatat tttgataggt ccattcagca
      721 ggtgggactt tatgtttgat tgccgcattt tctgcgggta agccaaatgc gtcccagcca
      781 atgggttgta atacattttt gccttgcatg cgttggaaac gactgatgac atcaccgatg
      841 gtgtagttac gcacgtgtcc catgtgtaag ctgccgcttg ggtaaggaaa catacttaga
      901 cagtaaaatt tttcgcgcag gggatcctct gaggccttaa aagtttgaga cttctgccaa
      961 taggcttgcg cttctgcttc aattttctgc ggattatatt gagattccat ggaaattcat
     1021 cgtcacttta agaaattcgg tgtctacagc atagcagacg ccccggcaga ataaaacagc
     1081 tgaatagcaa ccctaggaga ggccataatc caaatttaac ccagggtgtg gtgcccgtca
     1141 tactgtggaa agtgccgctt aatactcggg cttcaaattg tggaatggta gcactcaatg
     1201 ttcctttggg atcaataata gcggtgatcc cactcgttgt tgctcgcaat accatacggc
     1261 ctgtttcaag tgcgcgcatt tgggcaattt ctaaatgttg ataggggccc cacgattcgc
     1321 caaaccaacc atcttcactc aaggtaatga ttgcatctgc gtttcgtagg gtgttacgca
     1381 gtaattccgg aaaagcaatt tcataacaaa ttaaaggtac taatttgaga tcacctgctg
     1441 taattaaagg ttgttctgta ggacctgatg taaaactaga catgggcaga tcaaaaaaac
     1501 caatgagacc tcttaaccaa taatcaaatg gcagaaaatc accaaagggc accaagtgat
     1561 atttgtgata aatgccatga ccatctccca gagcaattaa tgaattatgg tattcttctt
     1621 tgctattgat cacttggatc cctgcaatta atgttgaatg gtgcgaaaac gcacgttttt
     1681 ctaattcatc gaaatagggc tttgaataag gcaaaggtaa aggaatcgca ctttctggcc
     1741 ataaaatcag atcggcaccc cattcttttt cggtgagttt tccgtaggtt ttttcagtcg
     1801 agccaatggg atcggtactg gtaaatttat cgaaaggctt aatatttccc tgtactaaac
     1861 gaactgtata caacttggga ttgggttttg taaaattgtg ctgttgcaaa aaatatccga
     1921 ttccccaaat gagaataatt aaaaaactcc ccgcagcttt gatttttcgg ctgcttatca
     1981 tcaaaacgat gagtgcacca ctcgtgagta gaacagccgt tgaaacgcca tagacgctca
     2041 taacaggcgc atagccactg agaggtgttg ttaattggct atagcctaaa tataaccatg
     2101 gaaaccctgt gaataaccaa cttcttaacc attcaaataa tacccaacag ctcggaaaac
     2161 cgagcagaca aaacgcaact gtgttgccac gaaaaaaatg ttttaagacg taaccctgtg
     2221 ttgcaataaa aagtgccagc acaatgacaa gtatagaggt tatcaaaacg gctaaaggaa
     2281 tattggtatt gccatagtgg tgaatgctca caaacaccca agagacacct accccaaaca
     2341 tgccaaggcc atatcccagt ccagacataa atgcctgttt agatgttgta ttcttttgcc
     2401 aaacccagag caaaatagca ggagagacga ttgccaatgg ccagagatgg tggggcgcaa
     2461 atgcccctgg cagtatggct cccgctagca tggctattat caataattga tacttattta
     2521 atgtgagatt taacatgttt ttttccaata tatgacagac ccttattcta cgtcaggtac
     2581 gcattatcta aaagcaataa caaagcacca agaaagactg agacagtatg cacatatctg
     2641 tttttaatga cagtgatttg ggcgactccg tatgtttcta tgtctcttaa tagcgataga
     2701 ctcacatatt gaaaataatt gataagaaag ttgatgttat aacatcctta aaccatactg
     2761 tggattaccc attttttaaa attcataggg agggcgtgat tatgtttaag cattggctgc
     2821 aaatgaatgt gtgcgataaa acattgcgtc agacgtattg gattaaggag aatggaaaat
     2881 gtgtcttaaa aaggatgata aagcggcagg tgagggtact ggtgtagaac gagagagcta
     2941 tcgccaaggt gacctgaacc caatagataa gctacaattc gcaaaatggg tgctaaaagg
     3001 catctttgtg ttttttgtga taagcattgc agttcgattg taccaagagg ggagtggcga
     3061 aaccttaatg gatatttgtc gaacaggatt attaccgata gcctcttttg tgattggcga
     3121 ttattttggg agcaggggtt agtgaaaata gccattttgt cgagacgagc gagattgtat
     3181 tctactcaac gcctggtaga agcagcgaaa atacgcggac atgaggttga ggtgattgac
     3241 accctgcgtt gttatatgaa tattgcttcg tataaaccgt ctattcatta caaaggtcgg
     3301 aagttggaag gatttgatgc ggttattccg cgcattggtg cttctattac attttatggc
     3361 acagcggttt tacgacaatg cgagatgatg ggcatttttc cactgaatga atctgttgct
     3421 atttcacgat cacgagacaa attacgttcc ttacaattat tagcgcgcaa aggcattggt
     3481 ttaccggtaa caggatttgc gcattctccg gatgaaacac aggatttaat tcatctagtg
     3541 ggcggtagtc cattggttat taaattactg gaaggtaccc aaggcatggg tgtggtgttg
     3601 gcagaaacac cgaaagccgc agaaagcgtt attgatgctt ttgcaggatt aaaagtgaat
     3661 attttagtgc aagaatttat taaagaagca gagtgtagcg atattcgttg ttttgtggtg
     3721 ggtagtaaag ttgttgcgac catgaagcgt caggcaaagc cgggtgagtt tcgatcgaat
     3781 ttacatcgag gaggcacgag cagtacagtc aagataacgc cagaagaacg ctcaactgcc
     3841 gtgcgtgccg caaaaattat gggtttaaat gttgctgggg ttgatatttt acgttctaat
     3901 aatgggccgg ttgttttgga agtgaattct tctccaggat tgcgcggtat cgaaaccacg
     3961 acaggattag atgtcgccag cattattatt gactttatag agcaaaatgc aaaacccaat
     4021 agtacaggga ctaaaggaaa aggataaggt tcttattctt tgtgtttcgc
//
LOCUS       DAORVT010000077         4024 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_17413_length_4024_cov_2.903250, whole genome shotgun sequence.
ACCESSION   DAORVT010000077 DAORVT010000000
VERSION     DAORVT010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17413_length_4024_cov_2.903250"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PK389_02275"
     CDS             <1..561
                     /locus_tag="PK389_02275"
                     /inference="COORDINATES: protein motif:HMM:NF015737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HQY22570.1"
                     /translation="DSVLSVGLNNYHGKRNMPLFNVTATSAYTGFQYGAKVKGGFTIP
                     CGQAEISPTAGVQYMHLNVGQYTEKGAEILNQQVSSTQISTVRAMLGARLADRSQEED
                     FFPEIHAFYLVDLKNPDVLITSRFVAGGGSFVSKSALPPKAGINVGASLTALVTDDFV
                     ISGGYDLEAKKSFRSHSASLKFKFLF"
     gene            complement(565..1989)
                     /locus_tag="PK389_02280"
     CDS             complement(565..1989)
                     /locus_tag="PK389_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQY22571.1"
                     /translation="MDPRFLPHNTHLEIPQQFSMRDPQLRQLKTQKYVYRSKLLDQFP
                     FNTPGIYTLGGGRQIGKSTLLKQWMLELLAKKVAPPAIAFFTGELINDHHALLNLVQT
                     ELEAMPDHNVHYIIIDEITYIKDWDKTIKYLADAGLLEKTILMLTGSDLNLIQEARMR
                     FPGRRGKASLVNFHLFPLSFQEVVLLKNKIPEINTVLHSKHFPSPTVIDALFSEFEDY
                     LKHGGFLTAINDLALDGTILKATLTTYSDWIRGDMLKRGKQEHFCREILTAIIKRYNT
                     QITWNSLVQDLSIDHPKTIADYAGVLESMDALFIQPVLLEDKLVAAPKKGKKMVFTDP
                     FIFHALRTWLFPVENAYENQIITALNNPELCSLLVEACVSTHYRRYYPTYYIKAEGEV
                     DVVYVDHGRFWPIEVKWTNQLRPKDIKQILKYPNGRIFSKNKHLSKLETTPIEPLPLA
                     LFNLPSPGASRHTLSFHGRGDSQS"
     gene            complement(2169..3524)
                     /gene="hslU"
                     /locus_tag="PK389_02285"
     CDS             complement(2169..3524)
                     /gene="hslU"
                     /locus_tag="PK389_02285"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010132035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HQY22572.1"
                     /translation="MDKNVFEVMTPQQIVQELDKHIVGQTAAKRSVAVALRNRYRRLR
                     VNEALRPEITPKNILMIGPTGVGKTEIARRLAKLVKAPFIKVEATKFTEVGYVGRDVD
                     SIIRDLVDVSVKLTREEALAKVQDRAEKTAKDRIINILVPTPSDVGEVHGTELEETQK
                     ASKKLFLKKLNSGELDDREIEIDLSHSAIGVEIMAPPGMEEMTNQLQNIFQSLSSDRT
                     RKRKLRIKDALKLLREEEASKLINDEDIKTRALERAEQAGIVFIDELDKVTQRSEIRG
                     GADVSREGVQRDLLPLVEGCTVTTKYGMIKTDHILFIASGAFHLSKPSDLIPELQGRL
                     PIRVELSALKTEEFVRILTEPDACLTVQYTALLETENVTLAFAKDGIQRLASIAWEIN
                     ERTENIGARRLHTVMERLLDDISFRATELASTTITIDKAYVEKQLGELIKDEDLSRFI
                     L"
     gene            complement(3524..>4024)
                     /gene="hslV"
                     /locus_tag="PK389_02290"
     CDS             complement(3524..>4024)
                     /gene="hslV"
                     /locus_tag="PK389_02290"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011079332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HQY22573.1"
                     /translation="NHVVIGGDGQVSQGNIILKGNAKKVRRLYQNNVIAGFAGGTADA
                     FTLFERFEKKLEAFQGHLTRAAVELAKDWRSDRALRRLEAVLIVADKQSSFMITGNGD
                     VIEPEHGLIAIGSGGAYAQAAASALLENTTLTARTIVEQSLKIAANICVFTNHNCIIE
                     ELEGQP"
BASE COUNT         1138 a          781 c          798 g         1307 t
ORIGIN      
        1 gacagtgttt tgtctgtcgg attgaataac tatcatggaa aacgaaacat gcctttattc
       61 aatgtaactg cgacatcggc ttatacgggg tttcaatatg gcgctaaagt taaagggggt
      121 tttacaattc cgtgtggtca agcagaaatt tcgccaacgg caggtgtgca atacatgcac
      181 ttaaatgtgg gtcaatatac agaaaaaggt gcggaaattt taaatcagca ggtaagttca
      241 acgcaaatca gtacagttcg tgcaatgcta ggtgcaagac tagcggatag aagtcaagaa
      301 gaagatttct ttcccgagat ccacgctttt tatcttgtag atcttaaaaa cccagatgta
      361 ctcattacct cacgatttgt agccggtggc ggttcgtttg tttcaaaaag tgcattgcca
      421 cccaaagcgg gaattaatgt cggtgcgagc cttaccgctt tggtcacgga tgactttgtt
      481 atcagtggtg gttatgattt agaagcgaaa aaatctttta ggagtcattc tgcttcgctt
      541 aaattcaagt ttttgtttta ggtcttagct ttggctgtct cctcttccgt gaaatgagag
      601 ggtgtggcgc gaagcgccgg gtgagggtaa attaaacaat gctaaaggca aaggctctat
      661 tggtgtcgtt tctagcttgc tcaagtgttt gttttttgaa aaaatacggc cattgggata
      721 ttttaaaatt tgtttgatat ctttaggtct cagttgattt gtccatttaa cttctatagg
      781 ccaaaaacga ccatgatcaa cataaacaac gtcaacttcg ccttctgctt taatgtaata
      841 ggttggataa taacggcgat aatgggtgga aacacaagct tcaactaaca gtgaacataa
      901 ctcaggattg tttaaggccg ttataatttg attttcgtaa gcattctcta caggaaataa
      961 ccaagtgcga agtgcatgga aaataaaagg atctgtaaaa accatttttt tgcctttctt
     1021 aggcgctgcc accagtttat cttccaataa cacaggttgt ataaacaatg catccatcga
     1081 ttctaataca ccagcataat ctgcaatggt tttcggatgg tcaatagaca aatcttgcac
     1141 cagggagttc cacgttattt gagtattata acgctttata atggctgtta atatttcgcg
     1201 acaaaaatgt tcttgtttgc cgcgctttaa catatcgccg cggatccaat cagaataagt
     1261 tgttaatgtt gcctttaata tcgtgccatc taaggctaaa tcattaatag ccgttaaaaa
     1321 tccaccatgt tttaaataat cttcaaattc actaaataat gcatcgataa cagttggcga
     1381 gggaaaatgt ttagagtgca atactgtatt aatctctgga attttatttt taagtaaaac
     1441 aacttcttga aatgataaag gaaaaagatg aaaattaacc agagacgctt tgccgcgtct
     1501 acccggaaaa cgcattcttg cctcttggat taaattcaaa tcagagcctg ttagcattaa
     1561 aatagttttt tctaataatc ctgcatctgc caaatattta atggttttat cccagtcttt
     1621 tatgtacgta atctcatcaa taataatata atgaacattg tgatctggca tcgcttctaa
     1681 ttcagtttgt actaggttca ataaggcatg gtggtcatta attaattcac ctgtaaaaaa
     1741 cgcaatcgct ggaggtgcga ctttttttgc gagtagttcc aacatccatt gtttcaataa
     1801 agtcgatttt ccaatttggc gtccgccacc taaggtgtag atccctggtg tgttaaaggg
     1861 aaattgatct aatagtttgg aacgataaac atatttttgc gtttttaatt gtcgtagttg
     1921 agggtcacgc attgaaaatt gttgcggtat ctctaagtgc gtgttgtggg gcaaaaatct
     1981 tggatccatg tcttagtatt aatcattgac taaatttagt caatgggtat tatctaaatt
     2041 tgatcattat gcaatgatca aatttagata attacaatat ttttaataat tatcaataag
     2101 ttacgaatac ctccctttga aaaaggggag cgacgagcga gcactgctcg catcggcggg
     2161 gggatttttt ataaaataaa tcgactcaaa tcttcatctt taatcaactc gcccaattgt
     2221 ttttccacat aagctttatc aatcgtaata gtggtacttg ccagttctgt tgctctaaaa
     2281 gagatatcat ctaataaacg ttccatcacc gtgtgcaatc ttcttgcgcc gatattttcg
     2341 gtacgttcat tgatctccca agcaatggag gcaagtcgtt gtataccatc tttggcaaaa
     2401 gcgagtgtca cgttttctgt ttctaataat gctgtgtatt gtacggttaa acaagcatca
     2461 ggctctgtta agattcgcac aaattcttcc gtttttaagg cgcttaattc aacacgaatg
     2521 ggcaatcgac cttgtagttc ggggattaaa tcagaaggtt ttgataaatg aaatgcgcca
     2581 gaagcgataa ataaaatatg atcagttttt atcataccgt attttgttgt aactgtgcat
     2641 ccttccacta agggcagtaa atcacgctgt accccttcgc gcgaaacatc tgcgccgccg
     2701 cgaatttcgg atcgttgtgt gactttgtct aattcgtcta taaagacaat gccggcttgt
     2761 tctgcgcgct ctaaagccct tgttttaata tcttcatcat taattaattt actggcctct
     2821 tcttcgcgta ataattttaa ggcatctttg atgcgcaatt ttcgttttcg tgttctatcg
     2881 ctcgatagac tttgaaaaat attttggagc tgatttgtca tttcttccat gccaggaggc
     2941 gccataattt cgacgccaat agcactgtga gaaagatcta tttcaatttc ccggtcatcc
     3001 aattcgcccg aatttaattt tttaagaaat aatttttttg aagccttttg cgtttcttct
     3061 aattctgtgc catgtacttc tcccacatcg gaaggggttg gaaccaaaat attgatgatg
     3121 cgatctttgg ctgtcttttc tgcccgatcc tgtactttgg ctaaagcttc ttctcgggtg
     3181 agtttaacag agacatcgac caagtctcga ataatggaat cgacatcgcg tccaacataa
     3241 cccacttcgg taaattttgt tgcttcaact ttaataaaag gcgcttttac cagttttgct
     3301 aatcggcgtg caatttcagt tttaccaaca ccggttggcc ctatcattaa aatattttta
     3361 ggcgtaattt ccggtcttag cgcttcatta acccgcagtc gtctgtaacg attgcgaagt
     3421 gctaccgcaa ccgaacgttt ggcagctgtt tgaccgacaa tgtgtttatc gagttcttgt
     3481 acaatttgtt ggggcgtcat gacttcgaat acgtttttgt ccattaaggt tgaccctcta
     3541 gttcttcaat aatgcaatta tggttggtaa acacacaaat attggctgca atttttaagg
     3601 attgttcgac aatggtgcgt gcagttaaag tcgtattttc aagtagtgcg ctggcggctg
     3661 cttgtgcata ggcgccacca gacccaatgg caattagtcc gtgctcaggt tcgataacat
     3721 caccgttgcc tgtgatcata aaagaacttt gtttatctgc cacaattaag acagcttcta
     3781 atcttcttaa agcacgatca gagcgccaat ctttggcaag ctctacagca gcacgggtta
     3841 aatgtccttg aaaagcctct agctttttct caaaacgttc aaataaggta aaagcatctg
     3901 ctgttcctcc ggcaaatcca gcgataacat tattttggta aagacgccga acttttttgg
     3961 cattaccttt taaaatgata ttgccctgag aaacttgacc atcaccgccg ataacaacgt
     4021 gatt
//
LOCUS       DAORVT010000078         4009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_17531_length_4009_cov_2.554881, whole genome shotgun sequence.
ACCESSION   DAORVT010000078 DAORVT010000000
VERSION     DAORVT010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17531_length_4009_cov_2.554881"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="PK389_02295"
     CDS             <1..697
                     /locus_tag="PK389_02295"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HQY22574.1"
                     /translation="AIPSVLFRKNDHVRQNAKMNKYSLAAWCLQVLITARKKVLPSYE
                     KGVVTLDFIKYVATLSSFSEGPKLAKEYLEKHGIHLIYVPHLPQTYLDGAVLQLSNGS
                     PVIGLTLRYDRIDNFWFCLCHELGHIGRHLDVKKDQIFIDDLTSHSFYYEDNRIENEA
                     DEWAESALIPDFQKFNSLLNGQRTSKKILELAAQMQVHPAIVVGSIRHKLKNFHLFSN
                     LIGHGEVRKHFKS"
     gene            926..1189
                     /gene="rpsP"
                     /locus_tag="PK389_02300"
     CDS             926..1189
                     /gene="rpsP"
                     /locus_tag="PK389_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004809941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HQY22575.1"
                     /translation="MVVIRLSRGGAKKRPFYHIVATDKRHRRDGGNCLERLGYFNPIA
                     GAEEPSLNRLNIKRDRVAHFVALGAQPSHRVAALLAEWDKKAS"
     gene            1168..1713
                     /gene="rimM"
                     /locus_tag="PK389_02305"
     CDS             1168..1713
                     /gene="rimM"
                     /locus_tag="PK389_02305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HQY22576.1"
                     /translation="MGQKGIVKTVLGTAEDHMVVMGRIGAPYGLKGWVHVQSYAETAS
                     SILSYKTWFLKIKGQWQPVEVLDARSHGKSFVAALVGYESIEKVELLKLSEIGVPRSE
                     LPVLDADEYYWSDLIGLSVVTEEGFVLGQLERLIETGSNDVLVVKGETREHLIPYILK
                     DYVLSVDLKARVICVAWDPEF"
     gene            1720..2466
                     /gene="trmD"
                     /locus_tag="PK389_02310"
     CDS             1720..2466
                     /gene="trmD"
                     /locus_tag="PK389_02310"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012323894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009019 - tRNA
                     (guanine-N1-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HQY22577.1"
                     /translation="MWCGIVTLFPEMFKALTDYGITRRAVERGLLELHYWDPRDFSTD
                     PHQSVDDRPYGGGPGMVMGVYPLRHAIIAARKAAGDQAKVIYLSPQGQKLSQERIRQA
                     AADSARFVFVAGRYEGVDERLVTLEVDEEWSIGDYVLSGGELAIMVVLDAITRWVPGA
                     LGDKDSALLDSFSEGLLDCPHYTRPEVVDGLQTPAVLLSGNHAEILKWRLKQKLGRTW
                     LRRKDLLGKLDLSLEQQKLLKEFIEETNII"
     gene            2482..2871
                     /gene="rplS"
                     /locus_tag="PK389_02315"
     CDS             2482..2871
                     /gene="rplS"
                     /locus_tag="PK389_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HQY22578.1"
                     /translation="MSTVIQQIEAEQLKQDVPVFRSGDVVVVQVEVKEGTRTRVQAYE
                     GVVIAKRNRGLNSSFTVRKISHGEGVERVFQTHSPIIKGIEVKRRGDVRKAKLYHLRG
                     LTGKAARIKERLAQRNTGVELDTAENA"
     gene            2878..3774
                     /gene="xerD"
                     /locus_tag="PK389_02320"
     CDS             2878..3774
                     /gene="xerD"
                     /locus_tag="PK389_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019529020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HQY22579.1"
                     /translation="MDNVVIVNEFVNALWLERAFSKNTLAAYQSDLKHFIVWLSGQAE
                     PKTLVTVSDIDVIGYLAYRTLQGTHARTNARVLSCLRTFYHYLLREKYCQQDPTVNIA
                     SPMIGRSIPKDLSEAEVESLLQAPNTEEPMGLRDKALLELLYACGLRVTELVSLTLEQ
                     VNLQQGIVRVMGKNDRERVVPFGEEAAVWLQRYLAVRSALLKTDRLCDAVFLSNRAVA
                     MTRQTFWYRIKHYARQVGIHKPLSPHTLRHAFATHLVNHGADLRVVQMMLGHASISTT
                     QIYTHVANSRLKALHLKHHPRG"
BASE COUNT         1135 a          689 c          999 g         1186 t
ORIGIN      
        1 tgcaatccct tcagtacttt ttagaaaaaa tgatcatgtg cgccaaaatg cgaaaatgaa
       61 taaatattct ttagcagctt ggtgcttaca ggttctaata acggctagaa aaaaagtttt
      121 gccatcgtat gaaaaaggtg ttgttacatt agattttata aaatatgtcg ccacattaag
      181 tagtttttct gaagggccaa agttagctaa ggaatatttg gaaaagcatg gtattcattt
      241 gatttatgtt cctcatctac cccagacgta tctagatggt gcggtgcttc agttgagtaa
      301 cggttcacct gttattggtc ttacgcttcg ttacgatcgg attgataatt tttggttttg
      361 cctttgtcat gagcttggtc atattggtcg gcacttggat gttaaaaaag atcaaatttt
      421 tattgacgac cttactagtc atagttttta ttatgaagac aatagaatag agaatgaagc
      481 ggatgaatgg gcagaaagcg ctctgattcc tgattttcaa aaatttaata gcttgctgaa
      541 tggccaaaga acttcaaaaa agattttaga gctggcagca caaatgcaag tacatcctgc
      601 cattgttgtg ggcagcattc ggcataagct aaagaatttt catctttttt ctaatttaat
      661 cggtcatgga gaagtgagga agcactttaa gtcatgaaaa tttatattgg tataaaaatt
      721 aatatgaaca ttttaggcga agacaaaatt tataatatga aaattgtttt aaaatcatta
      781 atgcaatcaa ttgtaattac atatttgccg cgataataaa agtaaactaa cttaaattta
      841 tggtggcgct tttatttctt ttttggtagg acttaggtac aatactcgct ttcgaaatcc
      901 aatattaaag gggttaatcc agcagatggt tgtaattcgg ttatcgcggg gcggcgcaaa
      961 aaaacgtcca ttttatcata tcgttgcgac agataagcgg catcgtcgag acggtggcaa
     1021 ttgtttagag cgattgggat attttaatcc cattgccggc gcagaagagc cgagcttgaa
     1081 tcgtttgaat atcaaacgcg atcgcgttgc gcattttgtg gcattgggtg ctcaaccttc
     1141 gcatcgtgta gcagctttgt tggcagaatg ggacaaaaag gcatcgtaaa gacggtattg
     1201 ggtacagcag aagaccacat ggttgtgatg ggtcgtatag gtgctcccta tggcttaaag
     1261 ggctgggttc atgtgcagtc ttatgcagag actgctagca gtattttaag ttataaaact
     1321 tggttcctta agataaaggg ccaatggcag cccgttgaag ttttagatgc ccgatcgcat
     1381 ggcaaaagct ttgttgctgc tttagtgggc tacgagagta ttgaaaaggt tgagctgtta
     1441 aagctttctg agataggcgt gccacgttca gagcttccgg tattagatgc ggatgaatat
     1501 tattggtctg atctcatcgg tttgtcggtt gtaacggaag aaggctttgt tttaggacaa
     1561 ttggaacgtc tgatagaaac gggctcgaat gatgttttag tggtgaaggg cgagacacgg
     1621 gaacatttga ttccttatat cttaaaggat tatgtgttgt cagtggattt aaaagcacgt
     1681 gttatatgtg ttgcctggga tccggagttt taagtattca tgtggtgtgg tattgtcacg
     1741 ttgtttccag agatgtttaa agcactcacg gattacggga taacacgacg agcggtcgaa
     1801 cgggggttgt tggagcttca ttattgggat cctcgcgatt tttcaaccga tccacaccag
     1861 agtgtagacg ataggcctta tggcggtggt cctggcatgg tgatgggtgt atatccattg
     1921 cgtcatgcga tcatcgcggc aagaaaggct gcgggtgatc aggcaaaagt gatttatttg
     1981 tcgccacaag ggcagaagct gtcgcaggag cgcattaggc aggccgcggc agattcagcg
     2041 cgatttgttt ttgtagcggg tcgatacgag ggcgttgatg aacggctcgt caccttagaa
     2101 gtggatgaag aatggtccat cggagactac gtgctaagtg gtggagaatt agccattatg
     2161 gttgtattgg atgccatcac gcgttgggta cccggcgcat taggggataa ggattcggcc
     2221 ttactcgatt ctttttcgga aggattatta gattgtccgc attacacgcg tccagaagtc
     2281 gtggatggtt tgcagacacc agcagttttg ttgagcggta accacgcgga aattttaaag
     2341 tggcggttaa agcaaaagct tgggcgtacc tggttaaggc gtaaagattt gttaggaaag
     2401 ttggacttga gtctcgagca acaaaaatta ttgaaagaat ttattgaaga aacgaatatt
     2461 atttaattag gagcgtaaaa catgagtacc gtgattcaac agattgaagc agaacaatta
     2521 aaacaggatg ttcccgtgtt tagatcgggt gatgttgttg ttgtgcaagt cgaagtgaaa
     2581 gaaggtacac gtacacgtgt tcaagcttac gaaggtgttg ttattgcaaa acgcaatcgc
     2641 ggattgaatt cttcttttac agtacgtaaa atttcgcatg gcgaaggtgt agagcgtgtt
     2701 ttccaaacgc acagtccgat tataaaaggc attgaagtaa agcgccgtgg agatgttcgt
     2761 aaggcaaaac tgtatcattt gcgtggtctt acgggtaagg cagctcgaat caaagaacgt
     2821 ttggcacaaa gaaatacagg cgttgaattg gataccgctg aaaacgcgta atctgcattg
     2881 gataacgtcg ttattgttaa tgaatttgtt aatgcgctct ggctggagcg cgcgtttagc
     2941 aaaaatacct tagcggcgta tcagagcgat cttaaacact ttattgtgtg gttatcaggg
     3001 caggcggaac caaaaaccct cgtcactgtc tcggatatcg atgtgatcgg ttatttagcc
     3061 tatcgcacgc tgcaaggaac gcacgcaaga accaacgcac gtgttctttc gtgtctaaga
     3121 actttttatc attatttgtt acgtgaaaaa tattgtcagc aagatccaac cgttaatatt
     3181 gcgagtccta tgattgggcg atcgattcct aaagatctgt ctgaagcaga ggttgaaagt
     3241 ttattacaag cacccaatac agaagagccc atgggtttga gagataaagc gttattagaa
     3301 ttgctttatg cctgcggctt aagagttacg gagttggtca gtcttaccct tgaacaagtt
     3361 aatttacaac aagggattgt gcgggtgatg ggaaagaatg atcgcgaacg tgttgttccg
     3421 tttggtgagg aggcggctgt ttggctgcaa cgttatttgg cggtacgctc ggcgttatta
     3481 aagaccgatc gtttatgcga tgctgttttc ctcagtaatc gcgctgttgc aatgacccgt
     3541 caaacctttt ggtatcgcat taaacattat gcaagacaag tggggatcca taaaccccta
     3601 tctcctcata ccttaaggca tgcctttgct acccacttag tgaatcatgg tgcggatctg
     3661 cgtgtggttc agatgatgtt gggtcatgcc agtatttcga ctacccaaat ctatacccat
     3721 gtcgctaaca gtcgactgaa agcgcttcat ttaaaacatc atcctcgagg ttaatcataa
     3781 attggtatat gattaaaaat gaaggcaggg agtcttcgat gcatcttcaa ttcatgagga
     3841 aagtgtttta tgaagcaagt tagcatgatt tttttgttat tgttgggctt tggtcaatgt
     3901 ttggccaata acacaaccga cacaaataac acaaatgaaa taagcaagct agagcaaggg
     3961 aatatcaaaa agcacctaaa tgccgcattt cctaaactag ttgttgaaa
//
LOCUS       DAORVT010000079         3977 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_17788_length_3977_cov_3.331464, whole genome shotgun sequence.
ACCESSION   DAORVT010000079 DAORVT010000000
VERSION     DAORVT010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17788_length_3977_cov_3.331464"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /gene="lysA"
                     /locus_tag="PK389_02325"
     CDS             <1..1146
                     /gene="lysA"
                     /locus_tag="PK389_02325"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014996338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HQY22580.1"
                     /translation="FQNQAHQINYAVKANSNLAVLNILAQLGSGFDIISEGELRRVLK
                     AGGLPEKIIFSGVGKTTAELKAALEIGVGCINVESKAELERLNNLAILLNKKATIAIR
                     VNPDVDPKSHPYISTGLQENKFGINITEASALYQQALTLPGIHIQGIAFHIGSQITTL
                     SPFLEALDKILALIGKLKKLGVAITHLNVGGGLGVHYQNETPPKPYAYVTAILAAIQT
                     MQKNAGISASNLIIHIEPGRAIAAEAGVLLTRIEYIKTQGSLKSFAIVDAAMNDLLRP
                     ALYNAWQEIVPLNTKKNPAIPARTYDIVGPVCETADFLGKDRVLSLQAGDLLMIRTAG
                     AYGFSMSSNYNSRPRAAEVMVDGDRFQVVRPRENIQDLLGSETVLTP"
     gene            1306..2751
                     /locus_tag="PK389_02330"
     CDS             1306..2751
                     /locus_tag="PK389_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013942880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQY22581.1"
                     /translation="MKPFIGRKQELKKLQDLSAFQRACLVVIRGRRRIGKSRLIAEFS
                     KNKLFLQFTGLAPIKKMKAQDQRNAFAGQLANNLAFPLPSFADWSDAFSYLSQNLTDK
                     PTVILFDEISWMGSKDRTFIPKLKAWWDLILQNHTNVILVLCGSVSTWISKNIINSTA
                     FFGRITLNIDLPELSLPEAYQFLKKNDFPGSLYDICKILAITGGIPWYLEQILPNQTA
                     DENIKRLCFEKDGLLVDEFDKIFHDLFKKKSLIYKKIISILSEGMRELVEIRELLGYA
                     RSGTLSNHIKDLIVSGFVSEHFTWSIKSGLLGKQKLYRLSDNYLRFYIKYIESNLPKI
                     KSNAYQALSLTNLPGWEAMIGFQVETLLLKNRPLLLKTLGINPADIVADNPYIQKPTV
                     RQKGCQIDYLIQTHTHNLFVCEFKFKRRELGLEIIESMQEKIRRFAAPRGFGVCPVLV
                     HLGGVTNSVHEKRYFYRIIDIADLVMPNIDT"
     gene            2958..3674
                     /locus_tag="PK389_02335"
     CDS             2958..3674
                     /locus_tag="PK389_02335"
                     /inference="COORDINATES: protein motif:HMM:NF001136.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HQY22582.1"
                     /translation="MSQSSTESQYTVIAFYKFIELADICSLQSAIAAFCKEHAILGTI
                     LLAHEGINATIAGSDQDIAAVVAFLHSHTPFAELQFRYTYADFNPFYKMKVRIKKEIV
                     ALRIPNIYPDKKTGIQVPPEEWNPVIADPETFLIDTRNTYECELGTFPGAHNPETDVF
                     RDFPAYVKQNLDPAKHKKIAMFCTGGIRCEKASSYLLEQGFSEVYQLQGGILNYLEKI
                     PAENSTWEGECFIFDNRITV"
     gene            complement(3731..>3977)
                     /locus_tag="PK389_02340"
     CDS             complement(3731..>3977)
                     /locus_tag="PK389_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007021208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HQY22583.1"
                     /translation="NKWQDQNGADRYSTDIIASTMQLMDSKGTGGSAPFDQNDQMSDM
                     PQDMTAMPSSNAQRSSAKAPEAAMTGSHDAFDDDVPF"
BASE COUNT         1232 a          790 c          733 g         1222 t
ORIGIN      
        1 tttcaaaatc aagctcacca aattaactac gccgttaaag caaatagtaa tttggctgtt
       61 ttaaatattt tggcgcaatt aggctctgga tttgatatca tttctgaagg cgaactaagg
      121 cgcgtcttaa aagcgggtgg cctacccgaa aaaataattt tctcgggtgt tggaaaaaca
      181 acagcagaat taaaagcagc cttagaaata ggtgtaggct gtattaacgt agagtctaaa
      241 gcggaactag aacgcttaaa taatttggca attctgctga acaaaaaggc aactattgcc
      301 attcgtgtaa acccagatgt tgatcccaaa agtcatccgt acatttcaac gggtcttcag
      361 gaaaataaat tcggtatcaa tatcacagaa gcttcagcgc tctatcaaca ggctttaaca
      421 ctgccaggta ttcatattca aggtattgca ttccatatcg gatctcaaat aaccacgcta
      481 tcaccctttt tagaagcact tgataaaata ttagcactga ttggaaagct caaaaaattg
      541 ggcgttgcta ttacgcatct caatgtggga ggcggtttgg gcgtacacta tcaaaatgaa
      601 acaccaccca aaccatacgc atatgttact gctatacttg cagctattca aacgatgcaa
      661 aaaaacgcag gaattagtgc ttccaattta ataatccata tagagcctgg tcgcgctatt
      721 gctgctgaag cgggtgtttt attaacacgc attgagtaca ttaaaacaca aggctcctta
      781 aaatcttttg caattgtcga tgccgcaatg aatgatttat taagaccggc actgtacaat
      841 gcctggcaag aaattgttcc tctaaacaca aaaaaaaatc ctgccattcc ggctcgtact
      901 tacgatattg taggtccggt ttgcgaaacc gctgattttt taggcaaaga cagagtgtta
      961 tctttacaag cgggagactt gctcatgatc cgcactgcag gtgcatatgg atttagtatg
     1021 agctccaatt ataattcccg accacgtgcc gcagaggtaa tggtggatgg cgatcgcttc
     1081 caggtggttc gcccccgaga aaatattcag gatctattgg gttccgaaac cgtgttgacc
     1141 ccataaaatg gtgcacaatc actattttcg ttataggctt cctggtttac aggtcaaaaa
     1201 tatgcccaaa acctgcattc gtcgattttg ggcatagaat ttgacttcaa ttgtatgcgc
     1261 atgttataca tttgtatgtt ttgagcataa aactggagat atcctttgaa accctttatt
     1321 ggacgtaagc aagaacttaa aaagcttcag gatctaagtg ctttccaacg tgcttgtttg
     1381 gtggttattc gcggccgccg ccgcattggc aaaagccgct taatagcaga attttctaaa
     1441 aacaagctct ttctccaatt tacaggacta gccccaatta aaaaaatgaa agcccaagac
     1501 caacgaaacg catttgctgg tcaactggcc aacaatctcg ctttcccact tccttccttt
     1561 gcggattgga gtgacgcatt ttcctattta tcacaaaatt taaccgataa acccaccgtt
     1621 atcttgtttg atgagatatc ttggatggga tccaaagatc gcacttttat tcctaaatta
     1681 aaagcttggt gggatttaat attacaaaat cacaccaacg taatattagt actatgcggc
     1741 tctgtttcta cttggatttc aaaaaatatt atcaacagca ctgcattttt tgggcgcatt
     1801 acgctcaata tagacttacc tgaattatca ttaccagaag cctatcaatt tttgaaaaaa
     1861 aatgattttc cagggtctct ctacgacatc tgtaaaattt tagccattac aggcgggatc
     1921 ccttggtatt tagaacaaat tttaccaaat caaacagcag atgaaaatat taaaagatta
     1981 tgttttgaaa aagatggtct gcttgtagat gaatttgata aaatttttca tgatctgttt
     2041 aagaaaaaat cgcttattta taaaaaaatt atttctattt tatctgaagg catgcgagag
     2101 ctagtggaaa ttcgggaatt attaggctat gcgcgaagtg gcacactgtc taatcatatc
     2161 aaagatttaa ttgtttctgg ttttgttagt gaacatttca cttggtctat taaatctgga
     2221 ttactcggaa aacaaaaact ttatcgtctt agcgacaatt atttacgttt ttacattaag
     2281 tatatagaat ctaatttacc caaaataaaa agcaatgctt accaagcatt gtctttaacc
     2341 aatcttccgg gctgggaagc aatgattggt tttcaagtag aaaccctact gcttaaaaat
     2401 cgtcctttat tgcttaaaac acttggcatt aatcccgccg atattgtagc agacaatcct
     2461 tatattcaaa aaccaaccgt ccgacaaaaa ggctgtcaaa ttgattattt aattcaaaca
     2521 catacccata atttattcgt ttgtgaattt aaatttaaaa gacgtgaact cggattagaa
     2581 attattgaat ccatgcaaga aaaaattcgt cgttttgcag caccccgagg atttggcgtt
     2641 tgtccggttc ttgtgcacct cggtggtgtt accaacagtg ttcatgaaaa acgttatttt
     2701 taccgcatta tcgatattgc tgatctcgtc atgccaaata tcgacacgta agtttttatt
     2761 tttcaaatag aaactgcaat tgcaatcagc tttttaatta aacttaacat gtttactccg
     2821 ccctaaatca agtattcata ccaaagaaca ttaattgtat cagggatcag ggcataattg
     2881 tctgtgtcca gacttgaccc atttgcttgt ttagccgaaa atagaccttt attttatcta
     2941 ccatgcaagg gtttctcatg tcgcagtcgt caaccgagtc gcaatatacg gttattgcat
     3001 tttataaatt tattgagcta gctgatattt gcagtttaca atcggcaatc gcagcgtttt
     3061 gcaaagaaca cgctattttg ggaaccattc tacttgcgca tgaaggcatc aatgccacaa
     3121 tcgcaggcag tgaccaagac attgcggctg ttgtcgcatt tctgcattcg catacacctt
     3181 ttgctgaact gcaatttcgc tatacttatg cagactttaa tcccttttat aaaatgaaag
     3241 tgcgtatcaa aaaagaaatt gttgcgctgc gtatccccaa tatttaccct gacaaaaaaa
     3301 cagggataca agttccacct gaagaatgga accctgttat tgctgatcct gaaacattct
     3361 tgattgatac gcgtaacact tacgaatgtg aattaggtac ttttccaggt gcacataacc
     3421 ccgaaacaga tgtgtttaga gattttcctg cttatgttaa gcaaaattta gatccggcta
     3481 aacataaaaa aattgctatg ttttgcacag gtggcattcg ttgtgaaaaa gcatcaagct
     3541 acttattaga acaagggttt tcggaagttt atcaactgca aggcggaatt ttaaactacc
     3601 ttgaaaaaat acctgctgaa aacagcacat gggaaggcga atgttttatt tttgataatc
     3661 gtattactgt gtaaaaataa gaacgtccct ccccctactg gaggagagac agtgttttat
     3721 ttatccatta ttaaaatgga acatcatcat caaatgcatc atgtgatcct gtcatcgcag
     3781 cttctggtgc tttagcagat gaacgctgag cattgctgct tggcatagca gtcatatctt
     3841 gtggcatatc actcatttga tcattttgat caaacggtgc gctaccgccg gtgccttttg
     3901 aatccatcaa ttgcatagta cttgcaataa tatctgttga ataacgatct gcaccatttt
     3961 gatcttgcca tttgttg
//
LOCUS       DAORVT010000080         3973 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_17819_length_3973_cov_3.400970, whole genome shotgun sequence.
ACCESSION   DAORVT010000080 DAORVT010000000
VERSION     DAORVT010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17819_length_3973_cov_3.400970"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            395..1225
                     /gene="dapF"
                     /locus_tag="PK389_02345"
     CDS             395..1225
                     /gene="dapF"
                     /locus_tag="PK389_02345"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HQY22584.1"
                     /translation="MLINFTKMHGLGNDFVVIDLISQAVHLRTAQIRRIADRHLGIGC
                     DQILFIEPPTRTTADFYLRIFNANGDEVEQCGNGARCAARFFYDMGFSNNPALEADCL
                     AGPLSFVIDDQGEVTVNMGIPTFHPNEIPFISKTEALQYPITVENAEHNISVLSMGNP
                     HAVIEVPDIALAPIKKLGSFISKHSYFPKETNVGFMQLIDKNQIYLRVYERGVGETFA
                     CGSGACAAVVAGIRLGLLSSPVSVVFSKGRLQIAWAGFNAPVMMTGPATRVFIGRFRL
                     "
     gene            1231..2139
                     /gene="xerC"
                     /locus_tag="PK389_02350"
     CDS             1231..2139
                     /gene="xerC"
                     /locus_tag="PK389_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020159057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HQY22585.1"
                     /translation="MMLQSVQAFLGSLRFERRSSEHTIASYKTDLMDALAFFEADHLN
                     TWQDIQESTIRALLAFRRNQKISPRTLNRQLSALRTFFRYLMRRGLITDNKAMRVLAL
                     KTSRALPRALDVDQMAKLLELPASNPFAIRDLAIMELLYSTGLRVSEIVSLNVRDLDL
                     KQQQTPVLGKGKKSRLALIGRCAVKALEQWLSIRTTLAEPVEPALFVNNKGKRLSARS
                     IQYRLYNIGIQQGVETRVTPHRLRHSFASHLLESSNDLRAVQELLGHADLSTTQIYTK
                     VNFQHLANVYDKCHPRARKQSEENKK"
     gene            2136..2642
                     /locus_tag="PK389_02355"
     CDS             2136..2642
                     /locus_tag="PK389_02355"
                     /inference="COORDINATES: protein motif:HMM:NF014639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HQY22586.1"
                     /translation="MKISLRFRTLLCLGGLLSFFFFLSTYLLEYSFGLEPCPLCYLQR
                     GILFIVSLVFCLGCLQNCKQHGRLVYGAILFVCSMLGGALALRQLWLQYISPVHESTC
                     FAGFQKMLELMPPWEVLQETLQGSQECGQIDFTFLGLPLSAWSFFSFMCLGMYVLILC
                     WLQIKRRI"
     gene            3051..3491
                     /locus_tag="PK389_02360"
     CDS             3051..3491
                     /locus_tag="PK389_02360"
                     /inference="COORDINATES: protein motif:HMM:NF016256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rsd/AlgQ family anti-sigma factor"
                     /protein_id="HQY22587.1"
                     /translation="MLGKKIEPSSTSHIQHLTQQWLELRQVVLVHYNELCATKDPEPN
                     MLQKFCQHLMDYISMGHFKMYEKFAEHHETNQTYSTGLDKTLLSNITINTETVLDFND
                     KYTDPKSLDALSKDLSRIGEILAHRMDWEDTLITGAAPRYSKGH"
     gene            complement(3528..3857)
                     /locus_tag="PK389_02365"
     CDS             complement(3528..3857)
                     /locus_tag="PK389_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HQY22588.1"
                     /translation="MTFVVTESCIKCKFTDCVEVCPVDCFREGPNFLVIDPDECIDCA
                     LCEPECPANAIYSEDDLKDDQKPFLALNAELSKKWPVITQNKGGLPDAEEWKDVPDKL
                     QYLERDE"
BASE COUNT         1162 a          873 c          737 g         1201 t
ORIGIN      
        1 tctcttgtct gttgcccctt ctcgaagtaa gttaaaacac tataatattt atagtaaaaa
       61 tctctcacct ccagtttgat acaaaataac atagcacgtc acttaaatct atgctgaaca
      121 actcaaaaaa aacacggttt tttattcggc tgttgagctt ttcctttttt taatctatcg
      181 tattcgtgat tgtggcaacc ttggttatat taacgacggc tctatgtttc agagaaagct
      241 aactttattg ttgataacaa caggatgtct cctgataacc gcctgtggcc aaggtgggcc
      301 gctttatcac cctgaccagc caccgcctat ttatgttcct cgcgattaac cacaatgttt
      361 gcgtctctgc ttaaacccct ttaagcttaa ggggatgctt atcaacttta cgaaaatgca
      421 cggtttaggt aacgactttg tcgtgattga tctgattagt caggctgtgc acttgcgtac
      481 agctcaaatt cgacgcattg cagatagaca tttgggcatt ggttgcgatc aaattttatt
      541 cattgaacca cccacacgaa caaccgccga tttttacctg cgcattttta atgccaatgg
      601 cgatgaagtg gagcagtgtg gaaacggcgc ccgatgtgct gcacgatttt tttatgatat
      661 gggttttagc aataaccctg cactagaagc agattgtctt gcaggacccc ttagctttgt
      721 gatagatgat cagggtgagg tgactgtcaa tatgggcatt ccaacattcc atccgaacga
      781 gatacccttt atcagtaaaa cagaagcact tcaatatcct atcactgtcg aaaatgctga
      841 acataatata agcgtactgt cgatgggcaa tcctcatgcc gttatagaag ttcccgatat
      901 tgcactagca cccataaaaa aacttggatc cttcatcagc aaacatagct attttcccaa
      961 agaaacaaac gttgggttta tgcaactcat tgataaaaac caaatctatc tacgtgttta
     1021 tgaacgtggc gttggcgaaa cttttgcatg tggctcgggt gcttgtgcgg ccgtggtagc
     1081 cggcatacgt cttggattat tgagcagccc cgtatcagtt gtattttcta aaggcaggct
     1141 gcagattgcg tgggcgggct ttaatgctcc ggtcatgatg acagggcctg caacacgcgt
     1201 ttttatcgga cgctttaggc tttaaaaaaa atgatgcttc aatctgttca ggcttttttg
     1261 ggatctcttc gatttgagcg gcgctcttcc gaacatacga ttgccagtta taaaacagat
     1321 ttgatggacg cactcgcgtt ttttgaagcc gatcacctca acacatggca agacatacaa
     1381 gaaagcacca tccgcgcctt attggccttt agacgcaatc aaaaaatatc accccgaacc
     1441 cttaatcgac aattatccgc actacgtact ttttttcgat atttaatgcg cagaggtctt
     1501 atcaccgaca acaaagccat gcgtgttctt gcactgaaaa caagccgtgc actaccacgt
     1561 gcactcgatg tagatcaaat ggcaaagctg ttagaattgc ctgcttcaaa tccgtttgca
     1621 attcgagatc tcgcgattat ggaactcctc tattctacgg gattacgtgt ttctgaaatt
     1681 gtctctttaa atgtgcggga tcttgacctt aagcaacaac aaactcccgt gcttggaaag
     1741 ggcaaaaaat caaggcttgc acttattggc cgatgtgccg ttaaggcttt agaacaatgg
     1801 ctctcgattc gcacaacgct tgcagaaccc gttgagcctg cattatttgt taacaataaa
     1861 ggaaaacgct taagtgcgcg atctatccaa tatcgtttgt ataatatcgg gatacaacaa
     1921 ggcgttgaaa cacgcgttac accacaccgt ttacgccaca gctttgcaag ccatttgtta
     1981 gaatctagca atgatttgcg tgctgttcaa gaacttttgg ggcacgccga tctgagtacc
     2041 acacaaattt atacaaaagt taattttcaa catcttgcga atgtctatga taaatgccat
     2101 cctagggcac gcaaacaatc tgaggaaaac aaaaaatgaa aataagcctg cggtttcgca
     2161 ccttgctttg tttaggtggt cttttgagtt ttttcttttt tttaagcact tatttgctcg
     2221 agtatagttt tggactagaa ccttgtccgc tttgctattt acaaagggga atattgttta
     2281 tcgtttcttt ggttttctgc ttgggttgct tacagaattg taaacaacac ggccgtttag
     2341 tttatggtgc gattttattt gtctgcagca tgctgggggg agcgcttgcg ttacgtcaac
     2401 tctggttaca atatatctcg cctgtgcacg aatccacttg ttttgcggga tttcaaaaaa
     2461 tgttggaatt aatgccgcca tgggaagttt tacaagaaac gctccaaggc tcacaagaat
     2521 gtggccaaat cgattttact tttctaggat taccattatc tgcctggtct ttctttagct
     2581 ttatgtgttt gggtatgtat gttcttattt tgtgctggct tcaaataaaa aggcggatct
     2641 aaaacccgcc ttttttaact tcatccttga agagagacct tccttgtcga tcaagtccat
     2701 ttaacaaaat tctatatcct tcctggatac actttaagaa taacaaataa attcatttat
     2761 tcaagccctc aaaaaaagaa aataattttt ttaatgggca acctccaaac cacactaaaa
     2821 aataacacat tgattttcaa acacttaaaa ttttctcgaa cgctttttcc tgttttccgt
     2881 tttttatatg gacattgtct tacaggactt tgagcgaaga ctcgacaaaa accgatcgat
     2941 tggcgttact gaaagaaaac ccacagccaa gatccttgac ttaatagaaa tagtggcttt
     3001 tactaaaaga atggacacat attttggtta acacggagga taccattttg atgttgggaa
     3061 aaaaaattga accgtcttca acttcacaca tccaacacct cacacaacaa tggctagaac
     3121 tcagacaagt cgtacttgta cactacaacg aactctgtgc aacaaaagac cccgaaccca
     3181 acatgctaca aaaattctgt caacatttaa tggattatat ttcgatgggt catttcaaga
     3241 tgtacgagaa atttgcagaa catcatgaaa ccaatcaaac atactcaaca ggtctcgaca
     3301 aaactttatt gtcgaacata accattaata cagaaacggt tcttgatttt aatgataaat
     3361 atacagaccc taaaagtctc gatgccttgt caaaagatct ctctcgaata ggcgaaatac
     3421 ttgcacatcg catggattgg gaagacacat taatcacagg cgccgcgccg cgctattcca
     3481 aaggccacta aaaaacttta ttatcgcttt ttaggtaatt gttaatacta ttcgtcgcgt
     3541 tctaaatatt gcaatttatc gggcacatct ttccattctt ctgcatccgg taacccgcct
     3601 ttattttgtg taatcacagg ccattttttg gataattccg cattcaatgc taaaaagggt
     3661 ttctgatcat cttttaaatc atcctctgag taaatggcat ttgccggaca ctccggttca
     3721 cataacgcgc aatcaataca ttcatccgga tcaatcacca aaaaattagg accttctcta
     3781 aagcaatcta cagggcagac ttcaacgcaa tctgtaaatt tacatttaat acaactttct
     3841 gttaccacaa atgtcattgc accactccaa aacttaataa aaagctaaaa ttctaacttt
     3901 ttgcgtatcg caccctatgc tattactgtt cactttatac gacactttga gaccagacaa
     3961 caagcgccaa ttg
//
LOCUS       DAORVT010000081         3970 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_17853_length_3970_cov_2.384419, whole genome shotgun sequence.
ACCESSION   DAORVT010000081 DAORVT010000000
VERSION     DAORVT010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17853_length_3970_cov_2.384419"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /gene="traT"
                     /locus_tag="PK389_02370"
     CDS             <1..450
                     /gene="traT"
                     /locus_tag="PK389_02370"
                     /inference="COORDINATES: protein motif:HMM:NF017619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HQY22589.1"
                     /translation="LQVGRSDLRAADHALNQGFGAALGGAVAGAAVGSLVSRQDNSKG
                     AIAGGLVGASVATVTDAMIQDVVYVVIADVQISERVGNSVTIKEKTRSRLKQGTNGLR
                     EVISTEKIEWKRYQTRVVSTANKVNLKFKQAAPELIQGLTRSITGIF"
     gene            507..965
                     /gene="dut"
                     /locus_tag="PK389_02375"
     CDS             507..965
                     /gene="dut"
                     /locus_tag="PK389_02375"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HQY22590.1"
                     /translation="MRSVQLKILDPKIGTSIPLPHYATAGSAGLDLRACLEQPLEILP
                     GATHLVPTGLAIYIRDRGLAGVMLPRSGLGHKHGIVLGNLTGLIDSDYQGEVMISCWN
                     RGKETFILEPGERLAQLVIVPVIQAQFEVVKEFFSETDRGAGGFGHTGRG"
     gene            970..2358
                     /locus_tag="PK389_02380"
     CDS             970..2358
                     /locus_tag="PK389_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HQY22591.1"
                     /translation="MTDFLILAEIFRAYDIRGVVNKSLTIEIVEAIGRALGTTVLARG
                     ETTIVVARDGRLSGPLLIDALSKGILATGCNVVDIGRVPTPVLYFATHHLNIPSGVMI
                     TGSHNPPDYNGLKMMVGGQALYGDGIQALLALIQKSDFSKGRGQKTERNIVDSYVQAI
                     KKDIHLSRPLKIVVDAGNGVTGDVVPVLFEALGCTVIPLFCEVDGHFPNHHPDPGQPK
                     NLVDLIAAVHAHQADIGLAFDGDGDRLGVVDNLGKIIWPDRQLILFAKEILKKQANAT
                     IIYDVKCTRHVSAVVESFGGQPLMWKTGHSLIKAKMQETGALLAGEMSGHLFFKDRWF
                     GFDDALYAGARLLEVLSEHNKETTVSEMFNTVPDSVNTPELLIPVTEQTKFSRVERLI
                     ALAQFPDATISTIDGLRVDFKDGFGLVRPSNTGPNLILRFEGDTTFALHRIQSEFRQL
                     LIDVDPLWELPF"
     gene            2375..3091
                     /gene="rph"
                     /locus_tag="PK389_02385"
     CDS             2375..3091
                     /gene="rph"
                     /locus_tag="PK389_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007224192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HQY22592.1"
                     /translation="MIRQSARARDELRDIRITRKFTKHAEGSVLIEFGDTKVICTASI
                     VQGVPKFLKGTNQGWITAEYGMLPRSTTERMQRENRVGPAGRTQEIQRLIGRSLRTAV
                     DLKALGEYTVNVDCDVIQADGGTRTASITGACVALADAFDLLRKQGMLKTNPLKFFVA
                     AVSVGIYKEMPILDLDYLEDSNAETDMNVVMTEQGGFIEVQGTAERSPFHIQELQGML
                     DLAKKGIATLIHKQKEALGL"
     gene            complement(3625..>3970)
                     /locus_tag="PK389_02390"
     CDS             complement(3625..>3970)
                     /locus_tag="PK389_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22593.1"
                     /translation="NYLIKTLLLDEMAKHTQVEFYPDPRNIKITSGDSLSDYLQTELF
                     FTKNVATKLITIPRDSASNKGVQFADMLSGLVQHHYENNNSDAFNLLVPFVQLETIYF
                     NESTSIRNVEIA"
BASE COUNT         1115 a          698 c          974 g         1183 t
ORIGIN      
        1 ttacaggttg ggcgttcgga tctccgagct gcggatcatg ccttgaacca aggttttggc
       61 gctgcattag ggggcgctgt tgcgggggct gccgttggaa gtcttgtgtc tcggcaggat
      121 aatagcaaag gtgcgattgc gggcggtctt gtgggtgctt cggttgcaac ggtgacagat
      181 gccatgattc aagatgtcgt ttatgttgtg attgccgatg ttcaaatttc cgaacgtgtg
      241 ggaaattcgg tcacaataaa agagaaaacc cgatcacgtt taaaacaagg cacaaatggt
      301 ctgcgggaag ttatctcaac agaaaaaata gaatggaaac gttatcaaac gcgggtggtc
      361 agtactgcca ataaagttaa tcttaaattt aaacaagctg cgcctgaatt aattcaagga
      421 ttaacgcgct cgattacggg aattttttaa tgcaatctgt tcaaagtcaa gagaaggcct
      481 atagaagtat tcagggtatt gatggtattc gcagcgttca actaaagatt ttagatccta
      541 aaattggcac ctcaatcccg ctgccgcatt atgcaaccgc aggttctgcg ggattagatt
      601 tacgcgcgtg tttagaacag ccattagaaa ttttgccagg cgcgacgcat ttagtgccaa
      661 cgggtcttgc gatttatatt cgcgatcgcg gattagccgg ggtcatgtta ccgcgttcgg
      721 gtttgggtca taaacatggc attgtgcttg gtaatttaac gggcctcatc gattcagatt
      781 atcagggcga agtcatgatc tcgtgttgga atcgcggaaa agagacgttt attttagaac
      841 ccggagagcg tctcgcgcaa ctggtgattg taccggtgat acaagcgcaa tttgaagtgg
      901 tgaaagaatt tttttcagaa accgatcgtg gcgcaggcgg ttttggtcat acagggcgtg
      961 gataaaaata tgacagattt tttaattcta gcagaaattt ttcgtgctta tgatattcgc
     1021 ggtgttgtga ataagagcct cacaatcgag attgtagagg ccatcggccg tgcgctaggt
     1081 acaacggtat tagcgcgtgg agaaacgaca atagttgttg cgcgagatgg ccgtttgtct
     1141 ggtccactat taattgatgc attgagcaaa ggcatattag caacaggctg taacgttgtt
     1201 gatattggta gggtgccaac accggttctt tattttgcca cacaccattt aaatattccg
     1261 tctggcgtga tgataaccgg aagccataat ccacccgatt ataatggtct taaaatgatg
     1321 gttggcggac aagcgcttta tggggacggg atccaagcgt tgcttgcatt aatccaaaaa
     1381 tcagattttt ctaaaggccg aggtcagaaa acagagcgca atattgttga ctcgtatgta
     1441 caagcgatca aaaaagatat tcatttgtcg agacccttaa aaatagttgt ggatgcagga
     1501 aatggtgtga cgggcgatgt tgttccggtt ttatttgagg cgcttgggtg taccgttatc
     1561 ccactttttt gtgaggtgga tggtcatttc ccgaatcatc accctgatcc gggtcagcca
     1621 aaaaatctcg ttgatctgat agcagctgtt catgcgcatc aagcggatat tgggttagca
     1681 tttgatggtg atggcgatcg ccttggtgtc gtagataatc ttgggaaaat tatatggccg
     1741 gatagacaat taatattatt tgcaaaagaa atcttaaaaa aacaggctaa tgcgacaatt
     1801 atttacgatg taaaatgcac aaggcatgtc agtgctgtgg tagaaagttt tggggggcag
     1861 cccttaatgt ggaaaacagg acattctctc attaaagcta aaatgcaaga aaccggtgcg
     1921 ttattagcgg gtgaaatgag tggacatctc ttttttaaag atcggtggtt tggttttgat
     1981 gatgcattat atgcaggcgc gcgtctttta gaagttcttt cagaacacaa taaagaaaca
     2041 acagtctcag aaatgtttaa taccgtgccc gacagcgtga atacgccaga attgctgatc
     2101 cccgttacag aacaaacaaa attttcgcgt gtggaacgat tgattgcatt ggcgcagttt
     2161 ccagatgcaa caatcagtac gattgatggt ttacgggttg attttaagga tggatttggg
     2221 ttagtgagac cttctaatac cggtcctaat ttaattttgc gttttgaagg tgatacgacg
     2281 tttgcgttac accgtatcca gtctgaattt cgtcaattgt taatagatgt tgatcctctg
     2341 tgggaattgc ctttttaaaa ttggagttaa agttatgata agacagagcg cacgtgcacg
     2401 agatgaatta agagatatcc gtatcacgcg caaatttacc aaacatgcgg aaggttctgt
     2461 attgattgaa tttggtgata ccaaagttat ttgtacagcg agcatcgttc agggcgtgcc
     2521 caagtttttg aaaggaacca accaagggtg gattactgcg gagtatggta tgttgccgcg
     2581 atcaaccaca gaacgtatgc agcgcgagaa tcgcgttggg cctgcgggtc gcacgcaaga
     2641 aattcaacgt ttaattggta gatcattgcg cacggcggtc gatctaaaag cattgggtga
     2701 gtatacggtt aatgttgatt gcgatgtgat ccaagcagat gggggtacca gaacggccag
     2761 tattaccggt gcgtgtgttg ctttagccga cgcctttgat ttgctgcgta agcaaggcat
     2821 gttgaaaacg aatcccttaa agttttttgt tgccgcagtt tccgtgggta tttataaaga
     2881 aatgccgatt ttagatttag attatctaga ggactccaat gccgaaaccg atatgaatgt
     2941 ggtaatgaca gaacagggcg gttttataga agtgcagggt actgcagagc gtagtccatt
     3001 tcatattcaa gagttgcaag gcatgttaga tctggctaaa aaagggattg cgacgttgat
     3061 ccataagcaa aaagaagcac ttggtttata agcaagacaa tggggtttat tgaccccatt
     3121 actcctcaaa atttcagggt ctatatgaat atcgtttttg tgccagtagg caagatattg
     3181 ttctgcattt aaatttaatt caggacatgt gtcaaagaga tcttgtaaat gtgttctaat
     3241 gtcgcgtttc cctctggtga cattatccca gtctgcatta tagatttctg aaatatgttc
     3301 tggtgttaca cctaagtctg tttcaatgtc tttccgccaa agatagcaac cttgagcatc
     3361 cattgcttta aaaatgacac cgtctacatc aaagatgatt gtctgcattc cagcattcta
     3421 agtctttaag ctcctaaaaa gtaggctggt gtcatacagt atttataaag tttacaaatt
     3481 gattagttgc gataggtgat cagtatgaca aaatcattgg aaatccattc tttaacacac
     3541 atttttagtg atagatgtat ttcaccggga ggttacgaac aaggtgacat tttattatat
     3601 ttttcagatg atttatagta ctgatcacgc aatctctaca ttgcgtatag atgtagattc
     3661 gttgaaatag atggtttcta actgaacaaa tggaacgagc aggttgaatg catctgagtt
     3721 attattttca tagtgatgtt gtaccaaccc ggatagcata tcggcgaact gaacgccttt
     3781 attggaagcg ctgtctctag gaatagtaat taattttgta gccacatttt tggtgaaaaa
     3841 taattctgtt tgtaaataat cacttaagct atcgccagaa gtgatcttta tatttctcgg
     3901 atcaggatag aattctactt gagtatgttt ggccatttca tcgagtaaaa gtgttttaat
     3961 aaggtaattg
//
LOCUS       DAORVT010000082         3961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_17914_length_3961_cov_6.991807, whole genome shotgun sequence.
ACCESSION   DAORVT010000082 DAORVT010000000
VERSION     DAORVT010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17914_length_3961_cov_6.991807"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            489..1964
                     /gene="msr(E)"
                     /locus_tag="PK389_02395"
     CDS             489..1964
                     /gene="msr(E)"
                     /locus_tag="PK389_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(E)"
                     /protein_id="HQY22594.1"
                     /translation="MSLIIKARNIRLDYAGRDVLDIDELEIHSYDRIGLVGDNGAGKS
                     SLLKVLNGEIVLAEATLQRFGDFAHISQLGGIEIETVEDRAMLSRLGVSNVQNDTMSG
                     GEETRAKIAAAFSQQVHGILADEPTSHLDLNGIDLLIGQLKAFDGALLVISHDRYFLD
                     MVVDKIWELKDGKITEYWGGYSDYLRQKEEERQHQAVEYELMMKERERLESAVQEKRQ
                     QANRLDNKKKGEKSKNSTESAGRLGHAKMTGTKQRKLYQAAKSMEKRLAALEDIQAPE
                     HLRSIRFRQSSALELHNKFPITADGLSLKFGSRTIFDDANFIIPLGAKVAITGSNGTG
                     KTSLLKMISERADGLTISPKAEIGYFTQTGYKFNTHKSVLSFMQEECEYTVAEIRAVL
                     ASMGIGANDIQKNLSDLSGGEIIKLLLSKMLLGKYNILLMDEPGNYLDLKSIAALETM
                     MKSYAGTIIFVSHDKQLVDNIADIIYEIKDHKIIKTFERDC"
     gene            2020..2904
                     /locus_tag="PK389_02400"
     CDS             2020..2904
                     /locus_tag="PK389_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(E) family macrolide 2'-phosphotransferase"
                     /protein_id="HQY22595.1"
                     /translation="MTIQDIQSLAEAHGLLLTDKMNFNEMGIDFKVVFALDTKGQQWL
                     LRIPRRDGMREQIKKEKRILELVKKHLSVEVPDWRISSTELVAYPILKDNPVLNLDAE
                     TYEIIWNMDKDSPKYITSLAKTLFEIHSIPEKEVRENDLKIMKPSDLRPEIANNLQLV
                     KSEIGISEQLETRYRKWLDNDVLWADFTQFIHGDLYAGHVLASKDGAVSGVIDWSTAH
                     IDDPAIDFAGHVTLFGEESLKTLIIEYEKLGGKVWNKLYEQTLERAAASPLMYGLFAL
                     ETQNESLIVGAKAQLGVI"
     gene            complement(3094..3705)
                     /locus_tag="PK389_02405"
     CDS             complement(3094..3705)
                     /locus_tag="PK389_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004889724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HQY22596.1"
                     /translation="MIEMNTRIYLRASTKDQDAERALQILQDLNQNLNLGETIVYVEN
                     YSGTKLDRPELNKLLSEANQGDTLLVESIDRLSRLTQRDFQELKRRIQEKGLRLVVAD
                     LPTTYQMIQTSDSITHSILELINNMLIDLLATMARLDNEKRIERIKQGLARSGYKPTG
                     KKANEAKHKRIKELLVVGNMTKEEIAKAANCGVATVYRVAKVI"
BASE COUNT         1260 a          676 c          797 g         1228 t
ORIGIN      
        1 ctgtttttta ttgtagattt ggaagaagag ggggtattaa tccttcctat accccaaaag
       61 gctgcctcat cgctaacttt gcaacagtgc ctaaaataag ccttatatcc aagcataaaa
      121 caaggttgtc tagacttctt ttaacagtaa agttatcata aaactgaatt ttatttttta
      181 ggtaagttta tgcattctat ccgcattcgt taagacacaa ctatttgcat agtgacacta
      241 ttttataatg gtgggctttt gttgtgtctt taagaatata tgcggatata taaagtaaaa
      301 gtatgcttaa tttataagta tgcttttagt gcatagtttc cagttataac ttaattgact
      361 agctatttgt ccaccctgtg gatgaatagc tttttttttg ggaggacact gtgatgctag
      421 cttttgtttt cacctaaatc ctgtttgctg cataaaaaat ttcaagagct aaacaggagt
      481 aaataaaaat gagtttaatt attaaagcga gaaacatacg cttggattat gctgggcgtg
      541 atgttttgga tattgatgaa ttggaaattc actcttatga ccgtattggt cttgtgggtg
      601 ataacggagc aggaaagagt agtttactca aagtacttaa tggcgaaatt gttttagccg
      661 aagcgacatt acagcgtttt ggtgattttg cacatatcag ccaactgggc ggaatcgaaa
      721 tagaaacggt cgaagaccgg gcaatgttat ctcgccttgg tgtttccaat gtacaaaacg
      781 acacaatgag tggcggagag gaaactcgtg caaaaattgc tgccgcattt tcccaacaag
      841 tacatggcat tctagcggat gaaccaacca gccaccttga tctcaatgga atagatctac
      901 ttattggtca acttaaagca tttgatggag cattacttgt tatcagtcat gaccgatatt
      961 ttcttgatat ggttgtagac aagatatggg agttaaaaga cggtaaaatt acggaatatt
     1021 ggggtggtta ctcggattac ttgcgtcaaa aagaagaaga gcgacaacac caagccgtag
     1081 aatatgagct gatgatgaag gaacgggagc gattagaatc tgctgtgcaa gaaaaacgcc
     1141 agcaagctaa tcgattagac aataagaaaa aaggagaaaa atccaaaaac tctaccgaaa
     1201 gtgctggacg acttgggcat gcaaaaatga ctggcaccaa gcaaagaaaa ctgtatcagg
     1261 cagctaagag tatggaaaag cgtttggctg cattagaaga tattcaagca ccagagcatt
     1321 tgcgttctat tcgttttcgt caaagttcag ccctagaact gcacaataag ttcccgatta
     1381 cggcagatgg tctgagctta aaatttggta gccgtactat ctttgatgac gctaacttta
     1441 taataccgct tggcgctaaa gtcgctataa ctggatcgaa tggaacaggg aaaacgtcct
     1501 tgttaaaaat gatatcagaa cgtgctgatg gattaaccat atctccaaaa gctgaaattg
     1561 gctactttac acaaacagga tataaattta acacgcataa atctgtgctc tcctttatgc
     1621 aggaagagtg cgagtacaca gttgcggaaa ttcgtgcagt attggcttca atggggatcg
     1681 gagcgaatga tattcaaaaa aacttatccg acttatcggg aggtgaaatc atcaaactgc
     1741 ttttatccaa aatgctttta ggaaaatata atattttgct tatggatgaa ccaggaaact
     1801 atcttgacct aaaaagtatt gccgcattag aaacaatgat gaagtcctat gcaggaacta
     1861 ttatcttcgt atctcatgac aagcaattgg tcgataatat tgctgacatt atctacgaga
     1921 tcaaagacca caaaatcatc aagacttttg agagagattg ttaatgatag ccaatctaat
     1981 ccgaacatta attattgaac tctttaaagg aaattaaaaa tgacaattca agatattcaa
     2041 tcacttgctg aagcacacgg cttgttgctt acggacaaaa tgaatttcaa tgaaatgggc
     2101 attgatttta aggtcgtttt tgctcttgat acaaaggggc aacaatggtt gctgcgtatt
     2161 cctcgtcgtg atggcatgag ggaacaaatc aagaaagaaa aacgcatttt agaattggta
     2221 aaaaaacatc tttctgtaga ggttcctgat tggagaattt catctacaga attagtggct
     2281 tatcccatac ttaaagataa tcctgtttta aatttggatg ctgaaaccta tgaaataatt
     2341 tggaatatgg acaaagatag cccgaaatac ataacatctt tggcaaaaac cttatttgaa
     2401 atccatagta ttcctgaaaa agaagttcgg gaaaatgatt tgaaaattat gaaaccttca
     2461 gatttaagac ctgaaatagc aaacaatttg cagttagtaa aatctgaaat tggtataagt
     2521 gagcaattgg aaacccgcta cagaaaatgg ttggataatg atgttctatg ggcagatttc
     2581 acccaattta tacatggcga tttatatgct gggcatgtac tagcttcaaa ggatggagct
     2641 gtttcaggcg ttattgattg gtcaacagcc catatagatg acccagcgat tgattttgct
     2701 gggcatgtaa ctttgtttgg agaagaaagc ctcaaaactc taatcatcga gtatgaaaaa
     2761 ctagggggta aagtttggaa taaactatat gaacagactt tagaaagagc agcggcctct
     2821 cctttgatgt atggtttatt tgccttagaa actcaaaatg aaagccttat cgttggagca
     2881 aaagctcagt tgggagttat ataatttaaa aatatgattg ctgagaactg ccttgttttg
     2941 aaacttggtt ggctttaatt agtttttagt attctttata gaaaatgcct cgatcaaggg
     3001 gcatttctaa caatcattta acataaaatt tcttatgtga agtaacctga atgcagcggt
     3061 gccccattgg gggcgcgttg agataagcct cttttagata actttagcaa ctcgatagac
     3121 agttgcaact ccacaattcg ctgctttggc aatttcttcc ttagtcatat tgccaactac
     3181 tagcaattct tttattcgtt tatgtttagc ctcatttgcc ttcttgcctg ttggtttgta
     3241 acccgaacgt gccaggccct gcttaatacg ttctatacgt ttctcattgt ctaggcgggc
     3301 cattgttgcc agaagatcaa tcaacatatt gttgatgagt tccaagattg aatgagtaat
     3361 gctgtcactg gtttgaatca tttggtaggt agtaggaaga tctgctacga ctaaacgaag
     3421 tcccttctcc tggatcctgc gcttcagctc ttgaaaatct cgttgggtta ggcgtgataa
     3481 gcggtcaata ctttcaacta acaatgtgtc accttgattt gcttctgaca gtagcttatt
     3541 caattcaggt cggtctaact tcgtaccact atagttttcc acgtacacaa tggtttcacc
     3601 caaattcaag ttttggttta gatcctgaag aatctgcaaa gccctttctg catcttgatc
     3661 tttagttgaa gctcgtagat aaatacgtgt attcatttct atcatttaat cttaattcta
     3721 ttagattaat gataatacta tcatttaatt attgcattaa gtctaatgat agatattgta
     3781 tgcgatttgg ctcgctatca ttcaggtata gtcttttgat aaaaaaagca tgactaaaag
     3841 ccatgccttg ggaattcgta taacgtgtat tatgttaatt ttagaaaatc ttcactacta
     3901 ttattttaaa ataagcctta tatccaagca taaaacaagg ttgtctagac ttcttttaac
     3961 a
//
LOCUS       DAORVT010000083         3953 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_17984_length_3953_cov_3.254233, whole genome shotgun sequence.
ACCESSION   DAORVT010000083 DAORVT010000000
VERSION     DAORVT010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17984_length_3953_cov_3.254233"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1460)
                     /locus_tag="PK389_02410"
     CDS             complement(<1..1460)
                     /locus_tag="PK389_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY22597.1"
                     /translation="MDIHNIHAQGTNKNVELPSVVITAPTGSSTESAGFRADNVLQGD
                     RLRRNRESNLGDTLSHELGVSSSSFGPGAGRPIIRGQDGTRVQVLENSLVTGDLSIIS
                     PDHAVAAETLNASRIEILRGPSTLMYGSGVSGGVVNVLNERIPDRLFKAPQANFEGRF
                     NSALEERAGALNASGSLGKMSWNIEGAKRKTNDIHIPGRAIANDQDSEVGFVRNSAID
                     SSNLSVGSSYIGERGFMGMSISRLDNLYGIPTPEGAKIDMGQTRYGLAGDLDNPLKGF
                     QQLRMRFSYNDYKHNELEKSGEIATRFKNDELDGRAELLHTPIAKWQGVMGVQLQNRK
                     FSAKGEEAFVPPSLSQSVGLFMLEKRVWQRWQFEVGGRVEHTTHNPQATLLQTRDFGL
                     YSVSAGSAWKFMDGYQIDLTATRGQRAPSTVALYASGIHVATSTYDQGNQTLRKETSN
                     NFDIALQKTKGIITGKVNLFYNHNDDYIFPKFLDNNG"
     gene            complement(1702..2022)
                     /locus_tag="PK389_02415"
     CDS             complement(1702..2022)
                     /locus_tag="PK389_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22598.1"
                     /translation="MIRKSLFLILALAILSVPVFSMAHTFTHFTQVDVLDVTETENGD
                     SVDLDEICFDCLALTALNFFLIASGFSLSDSAVYLRPSLLTTRLHSDNKYFPYYSRAP
                     PSPL"
     gene            complement(2092..2514)
                     /locus_tag="PK389_02420"
     CDS             complement(2092..2514)
                     /locus_tag="PK389_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HQY22599.1"
                     /translation="MFSKLHTTAEESIRHSGGRATAGRIGILAILLTEQQAITHREIE
                     QRLPQTLQLDRVTLYRTLEWLAGKNLIHKVTSDDRAWRYHANRELYSHQHAHFKCTRC
                     AQVICLNGLPIERSWPLPVGYRFHEIELTVKGLCADCS"
     gene            complement(3082..>3953)
                     /locus_tag="PK389_02425"
     CDS             complement(3082..>3953)
                     /locus_tag="PK389_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQY22600.1"
                     /translation="EKSRDSSKKKMESPTKPEPDKSNKILTKDNTNLQHSTQTSKSGI
                     TAARITQAIEEGEFNLYCQEIAPIKSSITSVSHYEILIRMHEEENNLMPPGSFLPLVD
                     QFKMMPKLDRWIINHVAKWLSAHPKTNFVFYINVAKDTLSDRAFPGFIQDQLQKIKTP
                     PSNLCFEIEVSDAEANTADTLIFTQKIRELGCLVSLCSFSESPKSLNLLEKMQVDYLK
                     IDGSLVCNILRDEEDLDKVKSINQLAHKAEIRTIAELVETDDIIVKLKEIGVDYAQGF
                     GVAMPHPFKDLES"
BASE COUNT         1122 a          817 c          793 g         1221 t
ORIGIN      
        1 ccattgttgt caagaaattt cgggaaaata taatcatcgt tatggttata gaatagatta
       61 actttccctg taataatccc tttggttttt tgcaacgcaa tatcaaagtt attggaagtt
      121 tcttttctca gagtttgatt gccctgatca taagtgctgg tagccacatg aatgccactt
      181 gcatacagag cgacagtact tggtgcgcgt tgtccgcgtg tggcggttaa atctatctga
      241 taaccatcca taaatttcca agcgctaccg gcggagacgc tatataggcc aaagtcacgg
      301 gtttgaagca gtgttgcttg tgggttatgt gtagtatgtt caacacgccc ccccacttca
      361 aattgccaac gttgccaaac acgcttttca agcataaata atccgactga ttgcgataaa
      421 ctgggcggca cgaaagcttc ttcacctttt gcggaaaatt tacgattttg taattgaacc
      481 cccattacac cttgccattt tgcaatgggc gtgtgtagta actcggctcg accatctaat
      541 tcatcatttt taaatcgagt tgcaatttca ccactcttct caagctcatt atgcttgtaa
      601 tcgttataac taaaccgcat tctgagttgt tggaatcctt ttaatggatt atccagatca
      661 ccggccaggc catagcgagt ctggcccata tcaattttcg ctccttcagg ggttggaatg
      721 ccataaagat tatctaacct tgaaatagac atacccataa agccacgttc accgatataa
      781 gaactcccta cggacaaatt actggaatcg attgcactat ttctgacaaa accgacttca
      841 ctgtcttgat cattcgcgat tgcacgacct gggatatgaa tatcattggt ttttcttttg
      901 gcgccttcaa tattccaaga cattttgccc aggcttcccg atgcattaag agcgccagcg
      961 cgttcttcca gagcagagtt gaagcgccct tcgaagttgg cttgtggtgc cttaaacaaa
     1021 cgatcaggaa tacgttcatt taaaacatta accacaccac cagatacgcc gctaccatac
     1081 atcaacgtgg atggtccgcg gagaatttct atgcgagaag catttaaggt ttctgctgct
     1141 actgcatgat ctgggctgat aattgaaaga tcgccagtaa caaggctgtt ttccaacacc
     1201 tgaacacgtg tcccatcctg tcctcgaata atcggacgac cggcgcctgg tccaaagctg
     1261 cttgaactaa cacccagttc atgagatagc gtatcaccta aattgctttc acgattccga
     1321 cgtaggcgat caccttgtag tacattgtca gccctgaatc ctgccgattc agttgaactt
     1381 ccggtaggcg cagtaataac aacactcggt aactcgacgt tcttgttggt accttgtgca
     1441 tgaatattat gtatatccat caagaaaaat aaaaggataa ggtccgctaa tttaaatagc
     1501 tggcctctac ccagccaatt cgtcttattt aaattgcaat tagaataagc catatagctt
     1561 ttccaacttt aaattgggta tttatcatgc aactgagttg tatgaatgaa tctaatcttt
     1621 catagaaaat aaaacataaa tcaagacaat caatagattg tccaagaact gattcagatt
     1681 aggattaaaa agcttaagaa actagagagg ggaaggtgga gcgcgtgaat aataaggaaa
     1741 gtatttgttg tcagaatgaa gtctggtcgt cagtaatgac ggtcgtaaat atactgctga
     1801 atcactcagt gaaaatcctg aagcaattaa aaaaaaattt agtgcagtaa gtgcaagaca
     1861 atcaaaacaa atttcatcga gatcaacact atcaccattc tcagtttcag tgacatcgag
     1921 cacatcgact tgtgtaaaat gggtgaaagt gtgtgccatc gaaaagaccg gcacagacaa
     1981 tattgctaac gccaaaatca gaaataagct tttgcgtatc atcagaaatt caagctggtt
     2041 ttctaaccat ggaagatcac ccgacctttt atttttgcaa agtgaatttc tttaactgca
     2101 atcggcacaa agacctttta ccgttaattc aatttcatga aatcgatagc cgaccggtaa
     2161 aggccagctt cgctcaatcg gtaggccatt caggcaaatt acctgtgcac aacgggtgca
     2221 tttgaaatgt gcatgttgat gtgaatacaa ttcacggttg gcatgataac gccaagcccg
     2281 gtcgtcactg gttactttgt ggatcagatt tttcccggcc agccattcga gcgtgcgata
     2341 taaagtcact cggtccaatt gcaaagtttg aggcaaacgc tgttcaattt cccggtgcgt
     2401 aatcgcttgt tgttccgtca gcaaaatggc caatattcca attctgcctg ccgttgcccg
     2461 gccgccgcta tgccggatcg actcttcagc ggtggtgtga agcttactaa acataaaaat
     2521 acttaaataa acgcaacaat gttgcagtca ttgtagtgga atgatttgct cggcgcaaca
     2581 tgtaatttgt ttgtgcaaga atccgtgctt tgatgcagtg ttgtggtcaa ataagcccgg
     2641 ctacttttta agttcttttt caaactctat tggtgccata tcactaatca ttgtatgaat
     2701 acgctgagtg ttatagtaac caatatgagt atcagtaata gcagattctt ttgtttgaca
     2761 gacattaccc cattcacgtt tcaagctaat gaagatatgt tcagtaggag aggttatccc
     2821 aagagttttc ctgtcgcaca tcatgccatg ctgtttaagc agttattgat aggcgtgact
     2881 aaatactgac tgccatcatc taaagagata tcattgttct gaaaattcac atcgacaaca
     2941 tgctgttaca tctggaaaat tacctgattc ctgggtacac tctgtaactg gcaattccat
     3001 ttctttatgc agcgtagata aatgaattat aaagtatttt atgacttaac ctcttttatt
     3061 ctggcgatac tttgtttgag tctatgattc aagatctttg aaaggatggg gcatagcaac
     3121 accaaatcct tgagcataat cgaccccaat ttcttttagc tttactatga tgtcatcagt
     3181 ttcaactaat tcggctattg ttctaatttc ggccttgtga gcgagttgat tgatgctttt
     3241 tactttatct aaatcttcct catcccgaag tatattgcaa actaaactcc catcaatttt
     3301 caggtaatcg acttgcattt tttcgagtag atttaatgat ttagggctct cgctaaagct
     3361 gcatagcgag accaagcaac ctagctctcg aattttttgc gtaaaaataa gcgtatcggc
     3421 tgtattggct tctgcgtctg atacttcaat ctcaaagcaa agattggagg gtggggtttt
     3481 tattttttgt aattgatctt gaataaagcc aggaaaagct cggtcactta aagtatcctt
     3541 agctacatta atataaaata caaagttggt tttggggtga gctgataacc acttggcgac
     3601 atgattgatt atccaccgat ctagttttgg catcatttta aattgatcta caagaggtaa
     3661 aaatgagcct ggaggcatca ggttattttc ttcctcatgc atacgaatga gaatctcata
     3721 atgtgaaact gatgtgatag aagatttgat aggggctatt tcctggcaat aaagattaaa
     3781 ttctccctcc tcaattgcct gggtgatgcg cgcagctgta attcctgatt tggatgtttg
     3841 agtactgtgt tgcaggtttg tattgtcttt agtcagtatt ttgttggatt tatctggctc
     3901 tggtttagtt ggactctcca ttttcttttt tgaggaatcg cggctttttt ctt
//
LOCUS       DAORVT010000084         3937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_18108_length_3937_cov_3.691911, whole genome shotgun sequence.
ACCESSION   DAORVT010000084 DAORVT010000000
VERSION     DAORVT010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18108_length_3937_cov_3.691911"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PK389_02430"
     CDS             <1..773
                     /locus_tag="PK389_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019349587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HQY22601.1"
                     /translation="GVKMRTINHGLKVVRRICNLAASEWMDEYGLTWMLNAPKIKLLP
                     EHDLRPPYPLSWEEQNRLFEELPAHLKAMALFAVNTGCREQEICQLRWDWEIKIPELP
                     GLVVFIIPGVFVKNGEDRLVVCNNIASSVIETQRDNHATHVFNYLGNPIKRMNATAWR
                     NGRKRAGLDQVRVHDLKHTFGRRLRSAGVSIEDRQDLLGHRSGKITTHYSAAELQNLY
                     DATNRVCEAGRGGVTLTVLRCANRELNNKDQQNIRGVL"
     gene            complement(869..944)
                     /locus_tag="PK389_02435"
     tRNA            complement(869..944)
                     /locus_tag="PK389_02435"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(909..911),aa:Phe,seq:gaa)
     gene            complement(1023..1292)
                     /locus_tag="PK389_02440"
     CDS             complement(1023..1292)
                     /locus_tag="PK389_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HQY22602.1"
                     /translation="MSHIVFCVKLQKEAEGLDRPPYPGKLGERIYKEVSKEAWQAWLK
                     HQTLLINENRLNMTDIKNRQFLATEMEKYFFGKGSATPTGFVAQK"
     gene            complement(1289..2389)
                     /gene="mutY"
                     /locus_tag="PK389_02445"
     CDS             complement(1289..2389)
                     /gene="mutY"
                     /locus_tag="PK389_02445"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013316705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HQY22603.1"
                     /translation="MILSQKILTWFDKHGRHDLPWQKNPTPYRVWISEIMLQQTQVTT
                     VIDYFERFMKRFPTLSKLAHAPLDDVFELWSGLGYYARAKNLHKTAQIINTTHKGKFP
                     TDYDAVLALPGIGRSTAGAILSISTQQCYPILDGNVKRVLARHFAVDGWNGNPITLEK
                     LWQYSEKITPKNRVKDYTQAIMDLGATVCTRSRPKCTACPIARSCAAKKLDRVKFYPM
                     PKPRAKIPTKATGVLMLVSHKHKCILLEKRPIKGIWGGLWSLPECPLQTDTKDWCTKT
                     FKLTLRTTHEWEPFRHTFTHFHLNIHPIFCEVRDQRKPKSIGLNYQWYSFEKTDTLGL
                     PAPIRRLIDKLKSLNQPTSMLSNTINIIEEVS"
     gene            complement(2389..>3937)
                     /locus_tag="PK389_02450"
     CDS             complement(2389..>3937)
                     /locus_tag="PK389_02450"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HQY22604.1"
                     /translation="LNATVTGSIAGHSLLDNIQKMPLFHSTEQSLPFRVVPYDITVEN
                     ATLHWKDAITNQDVRFKNVTLSAQKIPLGIVGIHAPLNIHFELQDATSEHTGSFSFKA
                     TWAFNAKAQQLDIQDLECNTNFPTLPFNSLLGKFQIHRLQNVPIIAGSFHIPSLSVHG
                     LSNFNTPLYPISAKTADLKGSFTYQMPMLAVTSFNLAFENAENVSGSFKTDLHAKTLK
                     TLNLTGSFVGKNLRIGTLPIPEIKTTLQANNGIFIFDNIKAQLASSHHQAKMQIDIRG
                     DVPEFKISGQMDSFEINELLARLDLKDKLYGNIKATVNLKTQGNSPDAWLQHLSGNAY
                     VHLTDGRVRGIDLSPLLRHAQSTLVMLKDTLSKRNSTNISAILTAELGEWRLQAMNVD
                     LLVTPFKHLKTNITFDNGRAYTTDFKLTHPEYTVNGHGMVDLTQKNVEYQALALLTHP
                     KQHPSESFSLFLKETPLKIHIKGSFDNLVVQPNLSHYADGAISLVHKGATEKAADNSL
                     EKLFGFP"
BASE COUNT         1083 a          718 c          908 g         1228 t
ORIGIN      
        1 atggagttaa aatgcgaact attaaccacg ggttaaaagt tgttcgcaga atctgcaatt
       61 tagctgctag tgaatggatg gatgagtatg gtttaacgtg gatgctaaat gcgcctaaga
      121 tcaagttgct gccagaacat gatctgagac cgccttaccc gctgagctgg gaagagcaaa
      181 atagactctt cgaagagttg ccagcacacc ttaaagcaat ggcattattt gccgttaata
      241 caggctgccg ggagcaggag atctgtcagt tacgctggga ttgggaaatc aagatccctg
      301 aattgccagg gctcgtggtt tttattattc ccggtgtatt cgttaaaaat ggagaagatc
      361 gtttggtggt ttgcaacaat atagcatctt ctgttattga aacccaaaga gataatcatg
      421 ctacgcatgt ttttaactat ttaggtaacc caataaaaag gatgaatgca acggcttggc
      481 gaaacggaag gaagagagcc ggtttagatc aagtacgtgt tcatgatctc aaacacactt
      541 ttgggcgtag attacgttca gcaggtgtaa gcattgagga tagacaagac ttactaggtc
      601 atcgctcggg aaagatcacg actcattatt cagcggcaga attacaaaac ctttatgatg
      661 caacaaatag agtttgtgaa gctggccgtg gtggagtcac gttaaccgtg ttgcgttgtg
      721 cgaaccgtga acttaacaac aaggatcagc aaaatatacg aggggtttta taatgaccta
      781 ctgtatggcg ttggttctag ctcccgcaaa actcccgcaa ggggttttgg gtgctttgct
      841 aaaattgacg ataagtgatt gattttattg gtggccagag ctggaatcga accagcgaca
      901 cgaggatttt cagtcctctg ctctaccaac tgagctatct ggcctaagag aataaagaac
      961 ctattagacc gtatcttggc acagtacgtc aagactaatc ttattttggt gtgtttgata
     1021 aattattttt gtgcaacaaa gcctgtcggt gtggcacttc ccttaccaaa aaagtatttt
     1081 tccatctcag tagctaagaa ttgtcggttt ttaatatcgg tcatattcag gcggttctcg
     1141 ttaatcagaa gtgtttgatg ctttagccaa gcctgccaag cttctttaga gacttcttta
     1201 taaatgcgtt caccgagttt gccagggtag ggggggcgat ctagcccttc agcttctttt
     1261 tgaagtttta cgcaaaatac aatgtgactc atgatacttc ctcgatgatg ttaatagtat
     1321 tgctgagcat ggatgttggc tgatttaagc ttttcaattt gtcaattaaa cgccggatag
     1381 gcgctggtaa gcccagggta tctgtttttt caaaagaata ccattggtaa tttaagccga
     1441 tggattttgg tttacgttga tctcgtacct cgcaaaaaat ggggtgaatg tttaaatgaa
     1501 aatgtgtaaa agtgtggcgg aaaggctccc attcgtgcgt tgtacgtaga gttagtttaa
     1561 atgtttttgt gcaccaatct tttgtatcgg tttgcagggg gcattccggg agactccata
     1621 atccacccca aatgccttta attggtcgtt tttccagcaa tatacattta tgtttatggg
     1681 aaaccagcat taacacaccc gtcgctttgg ttggaatttt tgctctaggt ttaggcatgg
     1741 gataaaattt gacgcgatcc aatttttttg ccgcacaact acgagcaata gggcaagctg
     1801 tacatttggg tcgacttctt gtgcaaaccg ttgcacccaa atccataatg gcttgcgtgt
     1861 aatctttcac gcggttttta ggcgttattt tttcgctgta ttgccaaagt ttttctaagg
     1921 ttataggatt gccgttccaa ccgtctactg caaaatggcg tgctaatact ctttttacat
     1981 taccgtccaa gataggatag cactgttgag tgctaatgga taagatcgcg ccagcggtcg
     2041 atctgccaat accgggcaat gccaaaacgg catcatagtc ggtgggaaat tttcctttgt
     2101 gtgtggtatt aattatttga gcagttttat gcaggttttt ggctctggca taatacccta
     2161 gaccagacca caattcaaaa acatcatcta aaggggcgtg tgcgagcttg gacagggttg
     2221 gaaaacgctt cataaaccgt tcaaaataat cgataacggt cgttacttgt gtttgctgta
     2281 acattatctc ggagatccaa acacgatagg gcgtcgggtt tttttgccaa ggcaaatcgt
     2341 gacgaccgtg tttgtcaaac cacgttaata ttttttgact aagaatcatt aaggaaaacc
     2401 aaacaatttt tccaaactat tatcagcggc tttttctgta gcgcctttat gaaccaagct
     2461 gatggcacca tctgcatagt gcgacaaatt gggttgtaca acgagattat caaaagaccc
     2521 tttaatgtgt attttcaagg gggtttcttt taaaaaaaga gaaaaagatt ctgaaggatg
     2581 ttgtttggga tgggttaata aagctaaagc ctgatattct acattttttt gtgtgagatc
     2641 aaccatacca tggccattca ccgtgtattc agggtgtgtt aatttaaaat cagtggtgta
     2701 agctctgcca ttatcaaaag taatattggt tttcaagtgt ttaaagggtg tgaccaagag
     2761 atcgacattc attgcttgca atcgccattc gcctaattca gcggtgagaa ttgcactgat
     2821 atttgtggaa tttctttttg aaagcgtatc tttcagcatc acaagcgtgg attgtgcatg
     2881 tcgtagcaag ggagagagat cgatacctcg tactctgccg tcagttaagt gcacataagc
     2941 gttacccgat aaatgttgta accatgcgtc tggtgaattt ccctgggttt ttaaatttac
     3001 cgttgctttt atattgccat acagtttatc ttttaagtcg agacgtgcta acaattcgtt
     3061 gatctcgaag ctatccattt ggccagaaat cttaaactct ggaacgtcac ctcgaatatc
     3121 aatttgcatt tttgcttggt ggtggctgct ggccagttgt gctttgatgt tatcaaaaat
     3181 aaaaatgcca ttatttgctt gcaacgttgt tttaatttca ggaataggga gtgtgcctat
     3241 tcgtagattt ttaccgacaa aagatccggt tagattaagc gtttttaatg ttttcgcgtg
     3301 tagatctgtt ttaaaagacc ctgagacatt ttctgcattt tcgaatgcca agttgaatga
     3361 tgtgacggct agcattggca tttgataagt aaacgagcct tttaaatcag ccgtcttagc
     3421 agatatgggg tataaaggtg tattaaagtt tgataatccg tgcacgctca ggctgggaat
     3481 gtggaagcta ccggcaataa tggggacatt ttgtagcctg tggatttgaa acttccccag
     3541 caatgagttg aaagggagtg ttggaaagtt ggtattgcat tcgagatctt gaatatccaa
     3601 ttgttgtgct ttagcattga atgcccatgt tgctttaaaa gaaaaacttc cggtgtgttc
     3661 agaagttgca tcttgcagtt caaaatgtat attcagcggc gcatgaatgc ctacaatacc
     3721 taaaggtatt ttttgagcgg ataatgtgac atttttaaat cgtacatctt ggttagttat
     3781 cgcatctttc caatgtagcg tggcattttc tactgtaata tcgtaaggca caacccgaaa
     3841 aggtaaagac tgttcggttg agtggaagag aggcattttt tgaatattat ctaaaagcga
     3901 atgcccagca attgatcccg tcacagtggc atttagg
//
LOCUS       DAORVT010000085         3895 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_18442_length_3895_cov_4.659115, whole genome shotgun sequence.
ACCESSION   DAORVT010000085 DAORVT010000000
VERSION     DAORVT010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18442_length_3895_cov_4.659115"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="PK389_02455"
     CDS             complement(<1..513)
                     /locus_tag="PK389_02455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQY22605.1"
                     /translation="MRSFTYQALDWIQPHLDSKESYSEKLAEHYPKLVDLQKTLMAFE
                     EEAGLSLSEIKALNKRLCSEEAKLSSAKKAMIEANLRLVLSIAKSYKGRGLAFSDLIQ
                     LGNLGLIKAVERFECRFGCKFSTYATGWIRQAITQAIADQGRNIRLPKHKIEEITHLN
                     RAKRQLLQEEG"
     gene            complement(675..1310)
                     /locus_tag="PK389_02460"
     CDS             complement(675..1310)
                     /locus_tag="PK389_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HQY22606.1"
                     /translation="MGYARASAPVSTQAEDIEMQILALRKAGCSKIYKEMTGIPSDRS
                     MLDTLIKELHENDTLVVWDLDRIARSFYHLFSLTHMFMQRNIGFISLNEHIDTTTSEG
                     RIVFNIFASLSEFERNNRREKNRNKVAMPGMQKRGNRKGMRPSSEAAACVAETLYLER
                     KLSATQIAKKLNIDKTRLYKYLHHRNVPLVSSGHPIGVSIAKYKGIRQNHP"
     gene            complement(1391..2494)
                     /locus_tag="PK389_02465"
     CDS             complement(1391..2494)
                     /locus_tag="PK389_02465"
                     /inference="COORDINATES: protein motif:HMM:NF023168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HQY22607.1"
                     /translation="MLQKNQGGIMGRIGISYQDVVKAIPKLQGQQRNPTVDNIREILG
                     TGSKSTIARFLREWKSQHGLKNEDDGTLPAELLEMLKALWGRFQEKTDTRAVAYQKEA
                     DSKIEQVDQQLNQQRQLQTDSQHKIHALEEQLHQQMAEKEQIKTALITEQQEKIKLME
                     RSTVLGSHLQESLGENKRLHQLLKQVQENLEHYQAATQQLRQEQSLLIEKQRSEYEQK
                     LSQLQIQTETIINEKSLYQAKYTELNKACELLEKKENALVLEYKEIERQHEASKIAYE
                     RIQQDHEKLLQDYREQSKNLESKQQTVIELQLMIKVDGDKIVSLEDALSKANDKIDTL
                     RHEFQFTSQAKANLEGQLKQLQAVLSSNKAAAA"
     gene            2669..3652
                     /locus_tag="PK389_02470"
     CDS             2669..3652
                     /locus_tag="PK389_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HQY22608.1"
                     /translation="MAKINQKTSTIPVLRNDDGITKALPPHDYIHAATSDNTRKAYQA
                     DIRHFTQWGGLLPTVVDIVMQYLQQHAGRLNPRTLVRRLTALRNWHLTQGFSDPTAHP
                     AIHKTLTGIKNVHGKPKNKAPALTLAFLIEMVSFLKKSERLMDCRSNALLQIGFFGAF
                     RRSELVALTWEAIHFVPEGIEILISRSKTDQAGEGQVCAIPYGDGTLCPVRALITWRD
                     RSCQSGFIFRQIQGGQINPDSIKAPQMNILIKSIAKACQLPDAELYSSHSLRRGFATE
                     ASKQGVPFGSILRQGRWRHEGTVLGYMDEGKRFEQNAAGLMLNHETSTDPG"
     gene            3627..>3895
                     /locus_tag="PK389_02475"
     CDS             3627..>3895
                     /locus_tag="PK389_02475"
                     /inference="COORDINATES: protein motif:HMM:NF025079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HQY22609.1"
                     /translation="MKRLQILGDAEIAALYAAPRFNAAERSHYFLLPEETLFSLKIEK
                     VNGRNTAAKLYFILQYGYFKAKHQFFTVSLKDAKKDVLFIMKHYLP"
BASE COUNT         1026 a          872 c          768 g         1229 t
ORIGIN      
        1 cccctcttct tgtaacagtt gacgctttgc gcgatttaaa tgagtgattt cctctatctt
       61 atgtttcggt aaacgaatat tgcgcccttg atctgcgatg gcctgggtaa tcgcttggcg
      121 aatccatcca gtggcatagg tcgaaaattt gcaaccaaag cgacattcaa accgctcaac
      181 ggctttgatt aaacccaaat tgcccaattg gatcagatct gaaaatgcca gaccccttcc
      241 cttataagat ttcgcaatgg acagcactaa acgcaaatta gcttcaatca ttgccttttt
      301 agcggagctt aacttagcct cttcgctgca aagacgctta ttcagggctt tgatttctga
      361 aaggcttaag cctgcttcct cttcaaacgc cattaaggtt ttttgcaagt caacgagttt
      421 agggtaatgt tccgctaatt tctctgagta cgattctttg ctatcaaggt gcggctgtat
      481 ccagtcaagt gcctgatacg taaaagaacg cataaagact tgccaaggca ttttagcctc
      541 ggtaatgggg ttctatgcat atgccccgaa aatgttccca ggatgtgaga gttacgctgt
      601 ttttcccctt atctgaaggc aaaatagcct gcaattattt aacctgaccc tcaattagct
      661 tttgcccata tactctacgg atggttttgt ctgatccctt tatactttgc tattgagaca
      721 ccaataggat gccctgaaga gaccaaagga acatttcgat ggtgtaagta tttataaagt
      781 ctcgttttgt ctatatttaa tttttttgca atttgcgtag cagataactt tcgttctaga
      841 taaagcgttt cggcaacaca agcagcagcc tcgctcgatg gacgcattcc ttttcggttg
      901 cctctttttt gcataccagg cattgcaact ttattccgat ttttttcgcg tctattatta
      961 cgttcaaact cggataaaga cgcaaaaata ttaaagacaa ttcggccttc agatgttgtt
     1021 gtatcaatat gttcattcaa actgataaac ccaatgtttc gttgcataaa catatgggta
     1081 agcgaaaaca aatgataaaa cgatctggca atacgatcaa gatcccagac aaccaaggta
     1141 tcattttcat gtaattcttt aataagagtg tctagcattg aacgatctga gggtattcca
     1201 gtcatctctt tataaatttt actgcaccca gcttttctca atgcaaggat ctgcatttcg
     1261 atatcttctg cctgcgtgct aacaggagca ctggcacgtg cgtaacctat tttaattttt
     1321 tagctccaaa actcacttta attttcctta tccagtctac caatgtattt attaaaatgg
     1381 aacatggttg ctatgcagct gccgccttat ttgaagataa aacagcctgc aattgtttaa
     1441 gctgcccctc taaattagcc tttgcctgag atgtaaactg gaactcatgc cgcagtgtgt
     1501 cgatcttatc gtttgctttg gataaggcat cttccaatga tacaatttta tcaccatcta
     1561 cttttatcat gagctgtaat tcaatgacag tttgctgttt agactccaaa tttttagatt
     1621 gttccctata atcttgcaac aatttctcat gatcttgctg tattctttca taggcaattt
     1681 ttgaggcttc atgctgtctt tcaatttctt tatactccaa aacaagcgca ttttcctttt
     1741 tttccaataa ctcgcaagcc ttattcagtt cagtgtattt tgcttggtaa agggattttt
     1801 cgttgattat tgtttcagtc tgtatttgta attgcgacaa tttttgttcg tactcactgc
     1861 gttgtttttc aatgagcaac gattgctcct gccgtaattg ttgtgttgcg gcctgataat
     1921 gctctagatt ttcttgcacc tgctttaaaa gttggtgtaa tcgcttgttt tctcctagac
     1981 tttcctgtaa atgagatccc agaacggtac tacgctccat taactttatt ttctcctgct
     2041 gctctgtgat taaagccgtt ttgatttgct ctttttctgc catttgctga tgcaattgtt
     2101 cttccagcgc atgaatttta tgctgagagt ctgtctgaag ctgcctctgc tgattgagtt
     2161 gctgatctac ctgctctatt ttagaatccg cttctttttg ataggccact gcccgagtat
     2221 ctgttttttc ttgaaatctg ccccataagg cttttagcat ttctaggagt tcagcgggta
     2281 atgtaccgtc atcctcattt ttaaggccat gctgtgactt ccactcgcgt aaaaagcggg
     2341 ctatggtgct tttactgcct gtgcccagaa tttcgcgaat attgtctacc gtcggatttc
     2401 gttgttggcc ttgtagtttt gggatggctt ttacgacgtc ttggtagcta atgcctattc
     2461 tgcccattat tcctccctga tttttttgta acatagaacg tagcgcataa taaaacgttt
     2521 tatagcaatt tgaaagccca atatttttct gaataacaat cgaccataat aaagattatc
     2581 gatgatatcc gaaagttgtc cgtgttatat tctgtaccat gcactcaaaa cccttggcta
     2641 aggacggatt tctaagacag gcgagcaaat ggcaaagata aatcaaaaaa cctccactat
     2701 tccagtatta cgtaacgatg acggcataac caaagctctg ccaccccacg attatattca
     2761 tgctgccact tcagataaca cccgaaaggc ctatcaagcc gatattcgac attttaccca
     2821 atggggcgga ttgctcccca ccgtggtgga tatcgtgatg caatacctcc aacaacatgc
     2881 agggcggtta aacccaagaa cactggtgcg taggctaacg gcgttgcgaa actggcatct
     2941 cacccaaggg ttctctgatc ccacggcaca tcccgccata cacaagacac tcacgggtat
     3001 caaaaacgta cacggtaaac ccaaaaataa ggcacccgcc ttgacgctgg cattcctgat
     3061 agagatggtc agctttttaa aaaaatcgga gcgtttaatg gactgtcgca gcaatgcctt
     3121 attgcaaatc ggcttctttg gtgcctttag acgcagtgaa ctggttgctt tgacttggga
     3181 agccattcac tttgtacctg aaggcataga gatcctgatt tcccgctcga aaacagatca
     3241 ggcaggagaa ggacaagtat gcgcgatacc ctatggggat ggaaccttat gccctgttcg
     3301 tgcattgatc acttggcgcg atcgaagttg tcaatctggt ttcatattcc gtcagatcca
     3361 aggcggacag atcaacccag attccatcaa agcccctcag atgaatattc ttatcaaatc
     3421 gattgccaag gcttgccaat taccggatgc cgagctttac agcagccaca gtttacgacg
     3481 gggttttgcc acagaagcca gcaagcaagg ggtacctttt ggttctatcc tgcggcaagg
     3541 tcgatggcgt cacgagggta ctgtactggg ttatatggat gaaggtaaac gatttgaaca
     3601 aaatgctgcg gggttgatgc tcaatcatga aacgtctaca gatcctgggt gatgctgaaa
     3661 tagctgcact gtatgcagca ccccggttta atgcagcaga gcgcagccac tattttttat
     3721 tacccgaaga gacacttttt tctctaaaaa tagaaaaagt caatggcaga aatactgcgg
     3781 caaaactgta ttttattctt cagtacggtt atttcaaagc aaagcaccaa ttcttcacag
     3841 ttagtcttaa agacgcaaaa aaagatgttt tgtttatcat gaagcactac cttcc
//
LOCUS       DAORVT010000086         3894 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_18459_length_3894_cov_3.257880, whole genome shotgun sequence.
ACCESSION   DAORVT010000086 DAORVT010000000
VERSION     DAORVT010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18459_length_3894_cov_3.257880"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2230
                     /locus_tag="PK389_02480"
     CDS             <1..2230
                     /locus_tag="PK389_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HQY22610.1"
                     /translation="GWSHTTPQQGIQNAAGEAGAWFADLCLYLFGYIAYLIPLLIGYG
                     SIRLFQNRFEPNDSHRFLWGLRIIGFGLTVVSGCGLASLHFGIWSKGMPVSAGGICGQ
                     IISAELNPFLGYLGSTLVLLAVFLTGVTLTTGLSWFGLIDKMGRRLLLGFWNFCAALP
                     CTLTYLKNWKQRYQERKGVLKSLKAERVDPTLGARKLKREVVIDAVPLKPKFLEMKEV
                     KPAIPSARILPVGSQLPLNTELPSVTLLEIAEPKNQTECYSPSKLEALSREVETRLLE
                     FGVQVNVVAVEPGPVITRFEMELAAGVKVSKITGLAKDLARALTVTRVRVVEVIPGKS
                     VVGLEIPNEYRELVRLREILESSQYIESPSHLSLALGKDIAGIPVVVNLAKMPHLLVA
                     GTTGSGKSVGINVMLLSLLYKAMPRDVRLILIDPKMLELSIYDGIPHLLTPVVTDMKD
                     AANALRWCVAEMERRYKLMAALGVRNLVGYNRKVCDAERANQPILDPFWLPASKGSSA
                     DVGAERPALSSLPHIVVIIDEFADMIMVVGKKVEELIARIAQKARAAGIHLILATQRP
                     SVDVITGLIKANVPTRIAFQVSSRIDSRTILDQQGAEQLLGHGDMLYLPPGTGVPIRI
                     HGAFVSDEEVHAVVKSLKQLGEPDYAIDVLGGSGDRSFSSGLGGFEEEESGEQDDLYD
                     SAVQIVAETRRASISHLQRRLKIGYNRAARLIETMEAAGIVSNMQSNGGREVLVQGSE
                     IS"
     gene            2362..3249
                     /locus_tag="PK389_02485"
     CDS             2362..3249
                     /locus_tag="PK389_02485"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQY22611.1"
                     /translation="MLWGPSFLFIKIAVGDIAPLTITALRTLIGVLLLYIVLKFRKVH
                     LWKHRKLWFHCFVIGIFANGIPWICFNFAVQTIPTSLSALINGTTPVLTIILANIFLK
                     DEQLTWGRGIGATVGLVGFCVLFLPTVFSSLQGTDLAVDMRGILLSFIGASSYAVGIV
                     YVRKNFPSVPPLVAPVIQLFSSLLYLIPLAFIFESPLLMIQTASLSSWFGVLGLAVFG
                     TAFAFIMYYRIIERQGATAVSTVTYLLPVFGTILGVTFLKETLDVQFLIAAILIFSGV
                     LIVNSVISVPFLKRNKIAT"
     gene            3382..>3894
                     /gene="lolA"
                     /locus_tag="PK389_02490"
     CDS             3382..>3894
                     /gene="lolA"
                     /locus_tag="PK389_02490"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HQY22612.1"
                     /translation="MKTKRLLALFNFALLCLAQSVFAVTAASAPTATDIAELKALLVP
                     VSSLQGNFQQVIKSEAGRQLQTSSGKMWLKKPGQFRWEVLGNEPRLIIADGKKVWDYD
                     KDLDQVTIQKLTKAQTAAPIFFLTGDVNSLDSDFKIKKLPLVDGHCLKNSDSCFELHP
                     KNSEGSFQWIK"
BASE COUNT         1045 a          678 c          929 g         1242 t
ORIGIN      
        1 gggttggtct catacgacac ctcagcaggg gatccaaaat gcggcaggag aggcgggtgc
       61 atggttcgcc gatttatgcc tctatttatt tggttacata gcctacctga ttcccctatt
      121 aattggctac ggaagtattc gacttttcca aaatcgtttt gagcctaatg attcgcatcg
      181 ttttttgtgg ggcttgcgca ttattggttt tggtttaacg gtggtttctg ggtgtggcct
      241 tgccagctta catttcggca tatggagcaa aggaatgccg gtgagtgcgg gtgggatttg
      301 cgggcaaatt atcagcgccg agcttaatcc tttcctgggt tatttgggca gtacacttgt
      361 cttattggcg gtatttttga cgggtgttac tctcactacc ggactttctt ggtttggctt
      421 aatcgataaa atgggcagga gattattgct ggggttttgg aatttttgtg cagcattacc
      481 ttgtacatta acttatttaa aaaattggaa gcaacggtat caagagcgaa aaggggttct
      541 gaaaagtctt aaagccgagc gtgttgatcc aacactgggt gcgcgaaaac taaaaagaga
      601 agttgtgatc gatgctgtac ctttaaaacc aaaattttta gaaatgaaag aagtaaaacc
      661 tgcgattccg agcgcacgca ttttacctgt aggttctcag ttgcccttaa atactgaatt
      721 accgtctgtg acgttattag aaatcgctga gcccaaaaat cagacagagt gttattcccc
      781 gagcaaatta gaagccttat cgcgagaagt cgaaacacgg ctattagagt ttggtgttca
      841 ggtaaacgtt gttgcggtcg agccaggtcc tgtgattact cgctttgaaa tggaattagc
      901 tgcgggtgta aaggttagta aaattacggg acttgctaaa gatttggcac gcgcattgac
      961 agtaacgcgt gtacgcgttg tagaagttat tcctgggaaa tccgttgtag gattagaaat
     1021 tccgaatgaa tatcgtgaat tggtgcgact gcgagagatc ttggaatcct ctcaatatat
     1081 agaatctcct tctcacctca gtttagcctt gggtaaggat attgcaggta ttcccgtcgt
     1141 tgttaatttg gcgaaaatgc cccatttgtt agttgcagga acaacgggtt cgggtaagtc
     1201 tgttggcatt aatgtgatgt tattaagcct attatataag gcgatgccaa gagatgtgcg
     1261 tttaatttta attgatccga aaatgttaga actctccatt tatgatggga taccgcattt
     1321 attaacgcct gttgtaactg atatgaaaga tgctgcgaat gccttgcgct ggtgtgtggc
     1381 agaaatggaa cgtcgttata agctgatggc ggctttaggg gttagaaatt tagtcggcta
     1441 taaccgtaag gtgtgtgatg cagaacgtgc aaatcagcct attttagatc ctttttggtt
     1501 acctgcttca aaggggagta gtgcggatgt gggtgctgaa agacctgctt tgagtagcct
     1561 gccgcatatt gttgtgatta tcgatgaatt tgctgacatg attatggttg ttggtaaaaa
     1621 agttgaagag ttaattgcac gcattgccca aaaagcgcgt gctgccggaa tccatttaat
     1681 tttagcaaca caacgaccgt ctgtagatgt gatcaccggt ttaattaaag ccaatgtgcc
     1741 gacacgcatt gcttttcaag tgtcttctcg cattgattcg cggactattt tagatcagca
     1801 aggtgcagaa caattgttag ggcatgggga tatgctctat ttgccaccgg gcacaggtgt
     1861 acccattcgt attcatggtg cttttgtgtc ggatgaagaa gtacacgcgg ttgttaagtc
     1921 ccttaaacaa ttgggtgaac cggattatgc gattgatgtt ttaggtggga gtggtgatcg
     1981 ttctttctcg tctggtttag gtggatttga agaagaggaa agtggcgagc aagatgattt
     2041 atatgattct gcagtacaaa ttgtcgctga aacacggcgt gcatcgattt cgcatttaca
     2101 gaggcgctta aaaattggtt acaatcgtgc tgcgcgatta atagaaacca tggaagcggc
     2161 aggcattgtg agcaatatgc aaagcaatgg aggtagagaa gtgctggttc aaggatcgga
     2221 aataagttaa gaacatgcag gaatcttgct aaatattcca aaattatgct agtattccaa
     2281 gtaaatttaa gcaaaatcac gggcgattga tctattatca tgaaacttca caaaggccta
     2341 ttctaaattt tgttgttatc tatgctgtgg gggccgtcgt ttctatttat taaaatcgca
     2401 gtcggtgata ttgcaccttt aacgataacg gcattacgta cattgatcgg cgtgttatta
     2461 ttatatattg tattgaaatt tagaaaagtt cacctttgga aacatcgtaa attatggttc
     2521 cattgttttg tgattggcat ttttgcaaat gggatacctt ggatttgttt taattttgcg
     2581 gttcaaacca ttcccacgag tctttctgcg cttattaatg gtactacgcc tgttttaacc
     2641 attattttag cgaatatttt tttaaaagac gaacaattaa cctggggtag agggattggt
     2701 gctaccgttg gattagtggg attttgtgtt ttatttttgc cgacagtttt cagttcgctg
     2761 caagggacag atctcgcagt cgatatgcga ggaatattgt taagctttat cggtgcttca
     2821 agctacgcag ttggaatagt gtatgtacga aaaaatttcc catccgtccc acctttagtt
     2881 gcgcctgtca tccagttatt ttcttcgctg ctttacttaa tacccttggc atttattttt
     2941 gaatcgccat tattaatgat tcaaacagca tctctgtcat cgtggtttgg tgtgttgggg
     3001 cttgccgttt ttggtaccgc ctttgctttt attatgtact ataggattat cgaacgacaa
     3061 ggtgcgacag ccgtatcgac ggtgacttat ctgttaccgg tttttgggac tattttaggg
     3121 gtcacttttt tgaaagaaac gctagatgta caatttttga ttgctgctat actcatattt
     3181 agtggagttt tgattgttaa tagtgtgatt tctgtgccat ttttgaagag aaataaaata
     3241 gcgacttagt agatccctga aatgaaataa agtaaaccag agccttaagg ccatttagtt
     3301 ttctagatat tattgttcct gccctttact gtgctaagat ttaacctatg atttacgggt
     3361 ggctcactac ggagtacgtt tatgaaaacc aagcgattat tagcgttatt taattttgcc
     3421 ttattatgtt tggcgcagag cgtttttgct gtaacagcag caagtgctcc aaccgcaaca
     3481 gatattgctg aattaaaagc cttattagta cctgtatcga gccttcaagg aaattttcaa
     3541 caagtcatta aaagcgaagc gggcagacaa ttgcaaacct cttctggtaa aatgtggctt
     3601 aaaaagccag gtcaattccg gtgggaagtt ttggggaacg agccaagact tatcattgcg
     3661 gatggtaaaa aagtttggga ttacgataaa gacttagatc aagtcaccat tcaaaaatta
     3721 accaaagctc aaaccgcggc acctattttt ttccttaccg gtgacgttaa ttcgctggat
     3781 agcgatttta aaataaaaaa gttaccactt gtagacggac attgtttaaa aaatagcgac
     3841 agctgttttg aattgcaccc taagaatagc gaaggatctt ttcagtggat caaa
//
LOCUS       DAORVT010000087         3874 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_18640_length_3874_cov_3.034302, whole genome shotgun sequence.
ACCESSION   DAORVT010000087 DAORVT010000000
VERSION     DAORVT010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18640_length_3874_cov_3.034302"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            290..1123
                     /gene="dapD"
                     /locus_tag="PK389_02495"
     CDS             290..1123
                     /gene="dapD"
                     /locus_tag="PK389_02495"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HQY22613.1"
                     /translation="MSDLQKIIEAAYDSRSTLSPASADPLLREAIDHVLADLELGKCR
                     VAHPLQPSLEKRTWQVNEWIKKAVLLYFCLNKSTSITGQFTQFYDKVPLRFTDTTSAE
                     FEALGVRIVPPATVRRGAYIAPNTILMPSFVNIGAYIGKGTMVDTWVTVGSCAQIGNH
                     VHLSGGVGIGGVLEPLQASPTIIEDNCFIGARSEIVEGVIVEHGSVISMGVYIGQSTR
                     IYDRETGEIHYGRVPANSVVVPGNLPSSDGKYSLYCAVIVKRVDDKTRAKTSINELLR
                     V"
     gene            complement(1128..1733)
                     /gene="tsaB"
                     /locus_tag="PK389_02500"
     CDS             complement(1128..1733)
                     /gene="tsaB"
                     /locus_tag="PK389_02500"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HQY22614.1"
                     /translation="MSHKINLLAIDTATDACSVALYCHGKMLERFCIRPREHTRLLLP
                     LIDDLLKEADISLNEIDVFAFGRGPGSFTGIRIACSVIQALSFSINKLVVPVSTLRAL
                     AQGVARTSGATQVFASLDARLESLYWGLFEMGAEGIMQPVSDEHLSLTDQIHLPTGSW
                     QKITGYPHAEDIARIAAYEYQVGNAVSAALALPIYLRDKIF"
     gene            complement(1822..2304)
                     /locus_tag="PK389_02505"
     CDS             complement(1822..2304)
                     /locus_tag="PK389_02505"
                     /inference="COORDINATES: protein motif:HMM:NF019028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone H1-like repetitive region-containing
                     protein"
                     /protein_id="HQY22615.1"
                     /translation="MAKKRKVVKAKTVKVAAKPKKRTAKKVAVKKTVAKKRVVKKADA
                     KKTVAKKRVVKKSDAKKAVAKKRVAKKAVAKKRVVKKAGVKKAVVKKAGAKKRVAKKA
                     VAKKAVAKKAVAKKAPKKRKTAKRKSAGAKGAVHKVAATGTRKRRTRKKAVAHVAVAA
                     "
     gene            complement(2386..3045)
                     /gene="adk"
                     /locus_tag="PK389_02510"
     CDS             complement(2386..3045)
                     /gene="adk"
                     /locus_tag="PK389_02510"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011072102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HQY22616.1"
                     /translation="MHHLRIILLGAPGAGKGTQAMLICEKLNLPKISTGDMLRAEISA
                     GTPLGHKVKQLMANGQLVSDELIIDLLRTRVAKPDCAEGFLLDGFPRNVLQAEALIKA
                     NIAIDHVVYIDVPDEDIIKRLSGRYTHLASGRTYHFLFNPPKSADKDDITGESLVQRE
                     DDTEVAVRTRLKIYHQETKPVIEWYKNVLKANQFHEIEGVGTVESIQDKILEMLKYKK
                     I"
     gene            3130..3852
                     /locus_tag="PK389_02515"
     CDS             3130..3852
                     /locus_tag="PK389_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HQY22617.1"
                     /translation="METPKEKLRPKPPLVNDAILSRESWKIFQVIAEFVEGYERLVHI
                     KPSVSVFGSARTKRDDTNYLLAESMGRKLSDAGYSVVTGGGPGSMEAANKGAYQGRSS
                     SVGLNIDLPTQESSNIYQDISLRFRHFFTRKVMFVKYATAYVVLPGGYGTLSEFAEIL
                     ALVQTGKTRQIPIILVNEPFWRPLLQWFKNTLLPDNMIDESDLTRWTFADSPEEVIQK
                     IQAFYNDKEIPTETLIEDKTDI"
BASE COUNT         1091 a          890 c          746 g         1147 t
ORIGIN      
        1 tttcaacctg aatgacagga ggaatcaccg cgcgttcttc tgtaaaaaat ttgtataacg
       61 gttgtgcaca aaaagataaa attaaaacac ccaccagcgc aatccataaa acaaaccgct
      121 ttgaaaacca agcgaaataa gcagtttttt gtttgagttt ctttaatcgt ttcagcataa
      181 ggcacctata cctatatcgg gatatctaag cacaatcatc gactttctgt ccacgatcgt
      241 gttattgttt ttaaaaacct aactttagca cctattggaa ccctttgcta tgagtgacct
      301 acagaaaatt atagaagcgg cttacgattc acgcagcaca ttaagccctg cctctgctga
      361 tcccctcttg cgagaagcga ttgatcatgt attagcagat ctcgaactcg gaaaatgtcg
      421 tgttgcacat cctctccaac cctccttgga aaaaaggaca tggcaagtca atgaatggat
      481 caaaaaggcg gtacttcttt atttttgctt aaacaaaagt acatccatta cggggcaatt
      541 cacccaattt tacgataaag tgccattacg gtttacagat accacctctg cagaatttga
      601 ggctctcggc gttcgcattg taccccccgc aacggtccgt cgtggtgcct atatcgctcc
      661 caataccatc ttaatgccaa gcttcgttaa cattggcgca tatataggca aaggtaccat
      721 ggttgatact tgggtaacgg taggttcttg tgcacaaatc ggtaatcatg tgcatctttc
      781 gggtggtgtt ggtatcggtg gcgtattaga gcccttgcaa gcaagcccta ccattattga
      841 agataattgt tttattggcg cacgttccga aattgtggaa ggtgtgatcg ttgaacatgg
      901 ctccgtcatt tcgatgggtg tttatattgg ccaaagcacc cgtatttacg atcgcgaaac
      961 cggcgaaatc cattatgggc gcgtgcctgc aaacagcgtg gttgtgcctg gcaatttgcc
     1021 aagcagtgat ggtaaatata gtctctattg cgcggttatc gtaaagcgtg tagatgataa
     1081 aacacgtgca aaaacgagca ttaatgagtt gttacgggtg tgaattttta aaaaatttta
     1141 tctcttagat atatcggcaa tgcaagcgct gctgagacag cattacctac ctgatactca
     1201 taggcagcaa tccgagcaat atcttctgca tgtggatacc ccgttatctt ttgccacgaa
     1261 cctgtcggca agtggatctg atctgtcaaa ctcaggtgtt catccgatac gggttgcata
     1321 ataccctctg cccccatttc aaataacccc caatacagtg actctaatcg ggcatccagg
     1381 ctcgcaaaaa cctgcgttgc accagaggta cgcgctaccc cttgtgctaa ggcacgcaag
     1441 gtagaaacag gtacgacaag cttatttata ctaaagctga gcgcttgaat aacactacag
     1501 gcaatacgaa tacccgtgaa gctgcctgga cctcgcccaa aggcaaaaac atcgatctca
     1561 tttagggata tatcggcttc ttttaataaa tcgtctatca acggtaacaa taagcgggta
     1621 tgctcgcgcg gtctaatgca gaatcgctcc agcattttac cgtggcagta cagcgcaaca
     1681 gaacaggcat ctgtggcagt atctattgct aagagattga ttttatgaga caaaatactg
     1741 aagtgttatc atagaatgct ttaatgacca ctaagcaccg cgcttaaaaa ataacgcgac
     1801 gcttaatggt ttcagtgtaa cttacgcagc cactgctaca tgagccactg cctttttacg
     1861 tgtgcgacgc ttgcgggtgc ccgttgcagc tactttatgt actgcacctt ttgcacctgc
     1921 cgatttacgc ttggcagtct ttcttttctt tggcgctttt ttagcaacgg cctttttagc
     1981 aacggctttt ttagcaactg cctttttagc tacgcgcttc ttagcaccgg cttttttaac
     2041 aacggctttc ttaacaccag ccttcttaac tacgcgcttt ttagcaactg ctttcttagc
     2101 tacacgcttt ttggcaaccg ctttcttagc atctgatttt ttaacaacgc gctttttggc
     2161 aactgttttc ttagcatctg cttttttaac aacacgcttt ttggcaactg ttttcttaac
     2221 agccactttc ttagcggttc gttttttggg ctttgctgct actttaacag tctttgcctt
     2281 tactacttta cgtttcttgg ccattctcga tctcctatgg cttaaactca attaattgga
     2341 caatacaaag atagcacaga tactgcacga gtacatacta cattgtcaaa tttttttata
     2401 tttaagcatt tctaaaattt tgtcttgaat agattcaacg gtgcctacac cttcaatttc
     2461 atgaaattga ttagctttta atacattttt ataccactca ataacaggct tcgtctcttg
     2521 atgatagatc ttcaaacggg tacgaacagc cacttcggta tcatcttctc gctgtactaa
     2581 tgactctcca gttatatcat ctttatctgc acttttgggg ggattaaata aaaaatggta
     2641 agtcctacct gacgcaagat gtgtatatct accacttaag cgcttaataa tgtcttcatc
     2701 cggcacatca atataaacaa catgatctat cgcaatatta gcttttatca acgcctctgc
     2761 ttgtaatacg tttcttggaa atccatcgag taaaaaccct tcagcacaat cgggtttggc
     2821 aacacgagtt ctcaataaat cgataatcag ttcatctgac accaactgtc catttgccat
     2881 aagttgtttt actttgtggc ctaatggcgt gcctgccgaa atttcggccc ttaacatgtc
     2941 gccggtagat attttgggca agtttaattt ttcacaaatc aacattgcct gagtaccttt
     3001 gcctgcgccc ggtgcaccta acaatataat ccgtaagtga tgcatctatt ttcctcaacg
     3061 ccaaaaagat gacattatat caagggttca atcccatcac cagagaaaaa gatatgctat
     3121 attgttaaaa tggaaactcc aaaagaaaaa ctgcgtccca aaccaccatt agtcaacgat
     3181 gcgatccttt ctcgcgaatc ttggaaaatc tttcaagtca tagcggaatt tgtagagggc
     3241 tatgaaaggc tcgtccatat aaaaccttct gtaagcgtat ttggttctgc aagaacaaaa
     3301 cgagatgata caaattacct cttagcagaa tctatgggtc gaaaattatc cgatgcgggt
     3361 tactcggttg tgacgggtgg cggacctggt tcaatggagg cagctaataa aggcgcttat
     3421 cagggtcgtt catctagtgt cggcctcaat atagatctcc ccacccaaga atcctctaac
     3481 atctaccaag atatttcatt gcgatttcga cattttttta cccgaaaagt catgtttgtt
     3541 aaatatgcta cagcctatgt cgtactgcca ggtggatatg gcacattaag tgaattcgca
     3601 gaaatactcg cattagtgca aacaggtaaa acacgccaga tacccatcat tttggtaaat
     3661 gaacccttct ggcgccccct acttcaatgg tttaaaaaca cgctgctgcc cgacaatatg
     3721 atcgatgaaa gcgatctcac tcgatggaca tttgctgact ctccagaaga agtgattcaa
     3781 aaaattcagg cattttataa cgataaagaa ataccaaccg aaaccttaat agaggacaaa
     3841 acagatattt agcctcaata tattgcgtcc tctc
//
LOCUS       DAORVT010000088         3841 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_18914_length_3841_cov_3.006339, whole genome shotgun sequence.
ACCESSION   DAORVT010000088 DAORVT010000000
VERSION     DAORVT010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18914_length_3841_cov_3.006339"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PK389_02520"
     CDS             <1..208
                     /locus_tag="PK389_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22618.1"
                     /translation="ATITEFPTLCLSILSHYKVRGFSELEGKPFYNLLMALFVDPEVV
                     QDFGMRFDAAVAASKAPQNKRLQL"
     gene            complement(214..1446)
                     /locus_tag="PK389_02525"
     CDS             complement(214..1446)
                     /locus_tag="PK389_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HQY22619.1"
                     /translation="MFLPKFKLPKINLSVKLISVFAFALLFGSQLPETVQSSCYAISL
                     TLKSILLFVLPVVIFSCLFSCLLTFKGKKAIGFMLGLLVIVCISNYLSTLIAYGISSL
                     ELVALDLNTIGNASSTQELQPLWSFELPNWIPNDYALYLGLGLGGIFAFFPTPRILRF
                     SAWARHSVTLFLEKGFIPVLPLFALGFILKMQYDGTLAQIIESYLLLLFIIVGIYVAY
                     LLFLFGLAANFKLKAWLTYLKNIFPVALMGFSTMSSLATMPITLNAAEKNTGALDISR
                     AVIPATVNIHMIGVSIAIPLMALTILLGYGHPLPSFSTYCLFALNFMIAQLAVAAVPG
                     GAILVMLPTLETHLGFTGEMSALITALYILFDPAVTISNVLGNSALVIFISKWFKGFT
                     TTEENKPIGQADPINQLL"
     gene            complement(1475..1684)
                     /locus_tag="PK389_02530"
     CDS             complement(1475..1684)
                     /locus_tag="PK389_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HQY22620.1"
                     /translation="MRALQCPNCQKPVEWTSEFPFRPFCSERCRMLDLGAWADNRYSI
                     PINAPVETEVPCRGGSPCLPSENDS"
     gene            1760..3214
                     /gene="tldD"
                     /locus_tag="PK389_02535"
     CDS             1760..3214
                     /gene="tldD"
                     /locus_tag="PK389_02535"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HQY22621.1"
                     /translation="MSVDQHHLEIARGLLLEPAGLSEQALQRVLDNVLGNAVDHADLY
                     FQNSRQESWVLEDGIIKAGNYNIDYGVGVRAMTGEKTGFAYSDDIILPALEQAAMTAK
                     TIAGIGGQNRIQAWHKAPVAQSLYGIDNPLESMIDAEKITLLRLLDAEARKMDTRVQQ
                     VVVSLSGVHDVILIVASDGTFSADVRPLVRLNVSVIVEEKGRREQASHGGGGRFNYAR
                     FLDADLGLNYAREAVRQALVNLEAEPAPAGMMPVVLGPGWPGILLHEAIGHGLEGDFN
                     RKGSSAFAGRIGERVASPLCTVVDDGTIMGRRGSLNVDDEGTPSQCTTLIENGILKGY
                     LQDKLNARLMGSQSTGNGRRESYAHLTIPRMTNTYLHAGNHFPEEIIKSVEKGLYAVN
                     FGGGQVDITSGKFVFSASEAYLIENGKITRPVKGATLIGSGPEILKQVSMVGNDLQLD
                     QGVGTCGKDGQSVPVGVGQPTLKIDLLTVGGTQQ"
     gene            3211..3822
                     /locus_tag="PK389_02540"
     CDS             3211..3822
                     /locus_tag="PK389_02540"
                     /inference="COORDINATES: protein motif:HMM:NF040244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HQY22622.1"
                     /translation="MNPERNPDWDNLKSTVADVLKQAKALGVDAAEVGISYESGLSIT
                     VRMGETETIEFNRDKSLDITVFKGKKKGTVSTSDIRKEAITSSLEAACRIATYTEEDP
                     CSGLADVEFLAKNMPDLDLYYPWAVSPEQAVLWAKECEASARAFDTRIINSEGATFST
                     HSRYSVYANTQGFMGAYPSTLHALNCTVIAKANDLYIEFTFKA"
BASE COUNT         1182 a          713 c          898 g         1048 t
ORIGIN      
        1 tgccacaata acagaatttc caaccttatg tcttagtatt ttatcgcatt ataaagtgag
       61 gggtttttca gagttagagg gaaagccttt ctataatttg ctgatggcac tttttgttga
      121 ccctgaagtt gttcaagatt ttggaatgag atttgacgca gctgtcgcag cttcaaaagc
      181 ccctcaaaat aagcggctac aactatagtt gcgttataag agttgattaa tcggatcagc
      241 ttggcctatt ggtttgttct cttcggttgt tgtaaagcct ttaaaccact tagagataaa
      301 gatcaccaat gcgctattac ctaatacgtt actaatggta acggccggat caaataagat
      361 atacaaagca gtaatgagcg cggacatttc gcctgtaaat ccaagatgtg tttctaatgt
      421 aggcagcatt acaagaattg cgccacctgg cacagcggcg actgcaagct gggcaatcat
      481 gaaatttaaa gcaaaaagac agtaagtact aaaactggga agaggatgcc cataacctaa
      541 cagaatcgtc aacgccatta atggaatcgc aatactcaca ccaatcatat gaatattaac
      601 ggttgccgga attaccgcac gcgagatatc aagagcaccc gtattttttt cagctgcatt
      661 tagagtaatg ggcattgttg caagactcga catggttgaa aagcccatca aggcgactgg
      721 aaaaatattt ttgagataag ttaaccaggc tttaagctta aaattcgcag ccaatccaaa
      781 taaaaacaac agataagcca cataaatgcc aacaataata aataataata ataaatagga
      841 ttcaataatt tgcgcgagtg taccgtcgta ttgcattttt aaaataaatc ctaatgcaaa
      901 aagcggcaac acagggataa accctttttc aagaaataga gtgacagaat gtcttgccca
      961 cgcactgaac ctcaggattc tgggtgttgg aaaaaaggca aatattccgc ctaatccaag
     1021 ccccaaatat aaagcataat cattaggtat ccaatttggt aattcaaaac tccagagtgg
     1081 ttgtagttct tgagtggatg atgcgttacc tatcgtgttc agatctaagg cgaccagctc
     1141 aagagaacta atgccgtaag caattaaagt agaaagataa tttgaaatac aaacaatgac
     1201 caagagaccc agcataaatc caatggcttt tttgccttta aacgttaata gacaagaaaa
     1261 taaacagcta aaaattacca cgggtaatac aaatagtaag atgcttttca gggtaaggct
     1321 aatcgcgtag caggaagatt gcacggtttc tggcagctgc gagccaaata ataaggcaaa
     1381 agcaaaaaca ctaattaatt taacagataa attgattttg ggtaatttaa actttggtaa
     1441 aaacataatc ttgtttgcct atatatttgt acagttatga atcgttttca gagggcagac
     1501 acggcgatcc gcccctacag ggaacttcgg tttctacagg tgcgttaatg ggaatactat
     1561 aacgattgtc agcccacgcg ccgagatcca gcatacggca ccgttcggaa caaaaagggc
     1621 gaaaagggaa ctcacttgtc cattccaccg gtttttggca atttgggcat tgtagggctc
     1681 tcataatgtt tgttattgtc tcataaggtc agaacgtttt ccagagacag acgctaaaat
     1741 tatttaagga actgagaata tgagtgtaga tcagcatcat ttagagattg ctcgaggtct
     1801 cttgctagag cctgcggggc tttctgagca agcgctgcaa cgcgtgttag acaatgtttt
     1861 agggaatgcg gttgatcatg ctgatctgta ttttcaaaat tcacgccagg aaagttgggt
     1921 attggaagac ggtattatta aagctggaaa ttacaatatt gattatggtg tcggtgttcg
     1981 tgccatgacg ggtgagaaaa caggttttgc ctattctgac gatattatat tgcctgcctt
     2041 agaacaagca gcgatgactg caaaaacaat tgcgggtatc ggaggacaga atcggataca
     2101 agcctggcac aaggcgcctg ttgcacagtc tttgtatggc atcgataatc ctttagaatc
     2161 catgatcgat gccgaaaaaa ttaccttact tcggctttta gacgcagaag ccagaaaaat
     2221 ggatactcgg gttcaacaag ttgtggtaag tctctcgggt gttcatgatg ttattttaat
     2281 agttgccagt gatggcactt tttctgcgga tgttcgtcca ttagttcgat tgaacgtcag
     2341 tgtgattgtg gaagaaaagg ggagacgaga acaagcaagc catggtgggg gtggacgttt
     2401 taactatgcg cgctttttag atgcggattt agggttgaat tatgcgagag aggccgttcg
     2461 ccaagccttg gtcaatttag aggcagagcc tgcaccggca ggtatgatgc cggttgttct
     2521 tggaccaggt tggccaggta tattgttgca cgaggctatt gggcatggtt tagaaggcga
     2581 ttttaatcgt aaaggtagct ctgcatttgc gggtagaatt ggagagcgcg tcgcatctcc
     2641 gctctgcacc gttgtagacg atggtacgat aatgggtcgt cggggttcgc tgaatgtgga
     2701 cgatgaaggc acgccatctc agtgtacaac cttaattgag aatggtattt taaaagggta
     2761 tttacaagat aaattaaatg ctcgtttaat gggcagtcaa tcaacgggta atggcagacg
     2821 cgaatcttat gcgcatttaa ccattccacg gatgaccaat acgtatctgc atgcaggcaa
     2881 ccactttcca gaagaaatta tcaaatccgt tgaaaaaggg ttatatgcgg ttaactttgg
     2941 aggtggtcaa gtcgatatca cctctggaaa atttgtcttt agtgcaagcg aagcgtatct
     3001 cattgaaaat ggaaaaatta ccagacctgt gaaaggcgca actctcattg gcagtggtcc
     3061 agagatttta aaacaggttt ctatggtcgg aaatgatctt cagctagacc agggtgtagg
     3121 tacctgtggt aaagatggac aaagtgttcc cgttggggtt ggtcaaccaa ctttaaaaat
     3181 agatttgctc acggtaggag gaacacaaca atgaatccag aaaggaatcc agactgggat
     3241 aatttgaaat caactgtcgc agatgtttta aaacaagcga aagccttggg tgtggatgct
     3301 gcagaggtgg ggatttccta tgaatcgggt ttatcgataa ccgttcgaat gggtgaaacg
     3361 gaaacgattg agtttaatcg cgataaatca ttagatatca ccgtttttaa gggaaaaaag
     3421 aagggaactg tttcaacatc cgacattcgg aaagaagcca tcacctcttc tctagaagct
     3481 gcgtgtcgta ttgcaactta caccgaagaa gatccctgtt ctggtttagc tgatgtggaa
     3541 tttttggcca aaaatatgcc cgatttagat ttatattatc cgtgggcagt ctctcccgaa
     3601 caagcggtat tatgggccaa agaatgcgaa gccagcgcac gcgcttttga taccagaatt
     3661 atcaattcag aaggcgctac tttttcaacc catagtcgat actctgttta tgcaaatact
     3721 cagggcttta tgggagcata tccgagtacg ctgcatgcac ttaattgtac ggtgatagca
     3781 aaggcaaatg acttgtatat tgagtttacc tttaaagctt aatcggctct aatggtaaat
     3841 g
//
LOCUS       DAORVT010000089         3838 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_18937_length_3838_cov_3.630716, whole genome shotgun sequence.
ACCESSION   DAORVT010000089 DAORVT010000000
VERSION     DAORVT010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18937_length_3838_cov_3.630716"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PK389_02545"
     CDS             <1..296
                     /locus_tag="PK389_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012800686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HQY22623.1"
                     /translation="AFTRGRELTDALQEIIPRQMFDIAIQAAIGAHIIARSTVKALRK
                     NVTAKCYGGDVSRKRKLLDKQKKGKKRMKQVGKVEIPQDAFLAILKVDKDKKG"
     gene            300..1136
                     /gene="lepB"
                     /locus_tag="PK389_02550"
     CDS             300..1136
                     /gene="lepB"
                     /locus_tag="PK389_02550"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007145689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HQY22624.1"
                     /translation="MRFSEILLTLTATTGVIWAVDALFFSAGRGVDALSGPINSGVKD
                     PWWVEYAKSFFPVLLIVFVLRSFLAEPFRIPSGSMRPTLLEGDLIVVNKYDYGLRWPI
                     LGNKIIKIGSPKRGDVIVFKHIKSDGESIDMIKRVVGLPNDHVQYKDKMIYINGEPVK
                     QAFQDEKQDVDPSGSSVTVRAFKEELGTHKHSIFTQPHGLYAPRYAYDDITVPEDQYF
                     VMGDNRDNSGDSRFWGFVKDIDIQGRAIAVWMSWDSTKYDAPWYRIIWNRIRWDRIGY
                     IP"
     gene            1146..1841
                     /gene="rnc"
                     /locus_tag="PK389_02555"
     CDS             1146..1841
                     /gene="rnc"
                     /locus_tag="PK389_02555"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019623266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HQY22625.1"
                     /translation="MAELWIEKAEHLAKKLNHAFSDIKLLHAAITHRSSSETNNERLE
                     FLGDSILNFVVAAELYARFPKAREGDLTRLRALLVRGETVAEVAREFGIGEYLSLGIG
                     ERKSGGHQRTSILADALEAIIGAIYLDSSLEVCRLCILRWYESRFNALVSGVSQKDAK
                     TQLQEYMQARRVPLPEYQVVQMGGDAHSPIFEVQCIFTLLKGPFIGVANTRRKAEQNA
                     ADLALEALKKNAR"
     gene            1831..2742
                     /gene="era"
                     /locus_tag="PK389_02560"
     CDS             1831..2742
                     /gene="era"
                     /locus_tag="PK389_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HQY22626.1"
                     /translation="MRDNIDTVCGYAAIVGRPNVGKSTLLNHILGEKLSITSRKPQTT
                     RHRLLGVKTLENAQIIYVDTPGMHIEEAHLLNKFMNKEARRALVDVDVIIFVIDSLKW
                     NAEDQLVFDLVTRSKCPIILAVNKVDTLLEKTALLPYCEALALKFKFAEIIPISAIRT
                     TNIDALERAVARYLPKSPHYFPEEQITDRTPRFRMAEIIREKLVRSLGEELPYSTTVE
                     IEMYKSEQSTKVSVISAIIWVEREGQKPIVIGKNGERLKEIGIRSRKDIEKIVGRQVH
                     LKLWVKVRGGWSDDERALKSLGYIDLE"
     gene            2745..3455
                     /gene="recO"
                     /locus_tag="PK389_02565"
     CDS             2745..3455
                     /gene="recO"
                     /locus_tag="PK389_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HQY22627.1"
                     /translation="MRRFECQLAYVLHTRPYRETSFLCEFFTADYGRISVVAKGVRRP
                     KSRGRALLQPFIPLMISCVGRGSLLTLTDFDAVGVAPVLTGRCAVSAFYLNELLFRLI
                     HHEDPNPDLFFCYQAALSELEKTTEVQIVLRLFEKSLLKMLGYELQLVKELRTGKPLN
                     PEGDYRFDPASGPVLVETGDLAIGVKSQDVFKGKSLLALAAGVLNDIVVLGDLKRLMR
                     LALAPHLGSKPLESRKLL"
     gene            3452..3838
                     /gene="acpS"
                     /locus_tag="PK389_02570"
     CDS             3452..3838
                     /gene="acpS"
                     /locus_tag="PK389_02570"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HQY22628.1"
                     /translation="MKIVGIGTDIVSTSRIKAIWDRFGSAFAKRILATSELVRLELSR
                     DPVSFLAKRYAAKEAVAKALGTGFRPEGILLREISVNNNALGRPYLEFSGEAEREMVR
                     CRVVESHLTLSDEKEFAVAFVILVSQ"
BASE COUNT         1118 a          607 c          967 g         1146 t
ORIGIN      
        1 aagcctttac gcgtggtcga gaattaaccg acgcattaca agaaattatt ccaaggcaaa
       61 tgtttgatat tgcgatacaa gcggccattg gtgcgcatat tattgcacgt tcaacggtaa
      121 aggctttgcg caaaaacgtt actgctaaat gttatggtgg tgatgtgtct cgtaaacgaa
      181 agctcttgga taagcaaaaa aagggtaaga agcgaatgaa gcaggtgggc aaggtggaga
      241 ttccacagga tgcctttttg gcgattttaa aagtagacaa ggataaaaaa ggataaaaaa
      301 tgcggttttc tgaaattttg ttgactttaa cagcgacgac aggtgttatt tgggctgtgg
      361 atgctttgtt ttttagtgca ggacgaggag ttgatgcgct ttctggtcct atcaattcgg
      421 gtgtaaaaga tccttggtgg gtcgagtacg caaaatcttt ttttcctgtt ctcttaattg
      481 tttttgtttt acgttcgttt ttagctgagc cttttagaat cccgtcgggt tcgatgcgcc
      541 ccactttact ggagggcgat ttgattgtcg tcaataaata tgattatggg ttaagatggc
      601 caatattggg caataaaatc attaagatag gttcgcccaa acggggtgac gttatcgttt
      661 ttaagcatat taaaagcgat ggcgaatcaa tagatatgat aaaacgcgtt gtgggattac
      721 cgaatgatca tgttcaatat aaagataaaa tgatttatat taatggagag cctgttaagc
      781 aagcttttca agacgaaaag caggatgttg atccctcagg ttcttcggtc acagttcgag
      841 cattcaagga agagctgggg actcataaac actcaatttt tacacaaccc cacggcttat
      901 atgcacctcg ctatgcttac gatgatatta ccgtaccaga agatcagtat tttgttatgg
      961 gtgataatcg agataacagt ggagatagtc gattttgggg ttttgtcaaa gatattgata
     1021 ttcagggtcg cgccatagct gtttggatga gctgggatag cactaagtac gatgctccgt
     1081 ggtatcgcat tatctggaat cgtattcgat gggatcgcat tggctatatt ccataaggaa
     1141 ataaaatggc agaactgtgg atagaaaaag cagaacattt agcaaaaaaa ctgaatcatg
     1201 ctttttcaga tattaagctt ttgcatgcgg cgataacgca tcgttcgagc agcgaaacca
     1261 acaacgaacg attggaattt ttaggagatt ctattttaaa ttttgttgtt gctgcagaat
     1321 tatatgcgcg atttccaaaa gcgcgcgaag gtgatttaac gcgattacgc gcattattag
     1381 tcagaggaga aacggtggcg gaagttgcaa gagaatttgg cattggggaa tacttaagtt
     1441 taggaatagg tgagcgtaag agtggtggtc atcagcggac gtctattttg gcagatgctc
     1501 tagaggcgat tatcggcgca atctatttag attcgagctt ggaagtttgt cgcctgtgta
     1561 ttttgcgttg gtatgagtct cgttttaatg ctttagtatc cggtgtttca caaaaggatg
     1621 ctaaaacgca attgcaagaa tatatgcagg cacgacgtgt tcctttacca gaatatcagg
     1681 ttgttcagat gggaggggat gcgcatagtc cgatctttga agtgcaatgt atttttactt
     1741 tgttaaaagg accctttatc ggggttgcga acacgcgccg aaaagcagaa caaaatgcgg
     1801 cagatttagc actagaggca cttaaaaaaa atgcgcgata atattgatac tgtttgtggt
     1861 tatgccgcta ttgtaggtcg ccccaatgtt ggaaaatcca cattgttaaa tcatattttg
     1921 ggagaaaaat taagtatcac ttccagaaaa ccacaaacca ctcggcatcg attgttgggt
     1981 gttaagacgc ttgaaaatgc gcaaattatc tatgtggata cacctgggat gcatattgaa
     2041 gaagcacatc tcctgaataa gtttatgaac aaagaggctc gtcgtgcatt agtcgatgtc
     2101 gatgtgatta tttttgtgat agacagctta aaatggaacg cggaagatca gttggtgttt
     2161 gatctggtta cgaggagcaa atgccccatt attttagcgg tcaataaagt ggatacgctt
     2221 ttagaaaaaa cagcgctttt gccgtactgt gaggcattag ctttaaaatt taagtttgct
     2281 gaaattattc ctatttcggc tatacgtaca acgaatattg atgccttgga gcgagctgtt
     2341 gcacgttatt tgccgaaaag tccacattat tttccagaag aacaaataac agacagaaca
     2401 cctcgattta gaatggcgga gattattcgg gaaaaattag taagatcttt aggggaagag
     2461 ttgccttatt ctactacggt tgaaatagag atgtacaaaa gtgagcaatc tacaaaagta
     2521 tctgtcatca gtgctattat ttgggtagag cgcgaggggc aaaaacccat tgtgataggt
     2581 aaaaacggtg aaagactcaa agaaattggt attcgatcac gcaaagatat tgaaaaaatt
     2641 gtgggacgcc aagtccatct aaagctttgg gtgaaagtac gtggtggatg gtcggatgac
     2701 gagcgagcct taaagagctt aggatatatt gatttggaat aaagatgcgt cgttttgagt
     2761 gtcaattggc ttatgtcttg catacgagac cgtatcgtga aaccagtttt ttatgtgaat
     2821 tttttacagc ggattatggt cgaatcagtg tggttgcaaa gggagtacgg aggccaaaat
     2881 ctcgaggtcg tgctttattg caaccgttta ttccgctgat gatttcgtgt gttggtcgag
     2941 gatcgttatt aacgttaaca gacttcgatg cggtcggtgt tgcacctgtg ttaaccggtc
     3001 gttgtgcggt aagcgcattt tatttgaatg aattgttgtt tcgcttaata catcacgagg
     3061 atccgaatcc tgatttgttt ttttgttacc aagctgcgtt aagcgaatta gaaaaaacga
     3121 cggaagtaca aattgtgttg cgattgtttg aaaaatcgtt attaaagatg ttgggatatg
     3181 aactccaatt agtaaaagaa ctgagaacgg ggaagccgct taatcctgaa ggagattatc
     3241 ggtttgatcc cgcaagcggg cctgttttgg tggaaacggg tgatcttgct atcggtgtaa
     3301 aatcgcaaga tgtgtttaaa ggaaaaagct tattagcctt ggcggcaggt gtgctgaatg
     3361 acattgttgt tttgggcgat ctgaaacgat taatgcgatt ggcattggcg ccccatttag
     3421 gctctaagcc actggaaagc agaaaattat tatgaaaatt gtgggtattg gtacagatat
     3481 tgtttcgaca agtcgaatta aagcgatttg ggatcggttt ggttctgctt ttgcaaaacg
     3541 aattttagcg acatccgagt tagtgcgttt agagttgtct cgagacccag tttcttttct
     3601 tgcgaagcgt tatgcagcaa aagaagcggt tgcaaaggcg ttaggcacag gttttaggcc
     3661 agaagggata ttattaagag agatcagcgt taataataat gctttaggac gaccttattt
     3721 agaattttcg ggtgaagcag aacgtgaaat ggtgaggtgt agggttgtcg aaagccattt
     3781 aacgttgtcg gatgaaaaag aatttgctgt tgcttttgtc atactagtga gtcaataa
//
LOCUS       DAORVT010000090         3830 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_19021_length_3830_cov_2.722384, whole genome shotgun sequence.
ACCESSION   DAORVT010000090 DAORVT010000000
VERSION     DAORVT010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19021_length_3830_cov_2.722384"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3830)
                     /locus_tag="PK389_02575"
     CDS             complement(<1..>3830)
                     /locus_tag="PK389_02575"
                     /inference="COORDINATES: protein motif:HMM:NF037238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="neuraminidase-like domain-containing protein"
                     /protein_id="HQY22629.1"
                     /translation="DEISKVIAILSKVNGDPDGSNTKAPFGDLSTLKVLDPYKTVLKD
                     YLSAADVTALEMATDIPKRLTMYWAHIEKRLLPVLRETFVQQHLIAAFKVEADIVKLM
                     LQETVILQTCLNVETAPANDKTYAEQYILMHRFTWLVGKLKLSGKELTHFENNPNFSN
                     FNWKALDFKLWLRIADFAALRDSLPPAKSSLFPAESSLFPAESSLLSLFETAKKGGDI
                     TQAIVDVTAWDKINVAYFVAQHTSADFLNEITLINLQRKLALSEKIGVSIEELESWTT
                     DSVSYEQSQDIKRTLKAKYSEDAWVEVSTAVHNRLRMHLRDALVAYLLQKSEIQKIGL
                     KSANDLYAYFLIDVEMGACMLTSRLKQAIALVQLFVQRCLLNLEESNKMEESHKIPPE
                     LINKKHWEWMRLYRVWEANRKVFLYPENWIEPELRDNKSPFFKDLESELLQGELTDEI
                     AEQKFMHYLEKLDEVSRLDICGIYEDHDAKEMHVFGRTFNTPPKYFYRKLDKKTQVWT
                     AWEHVPLDIQGNEDGDSAGVHLMPVVWNRRLYLFWPIFTEKSDKEKIERDREPHKQWK
                     KRKADSEAQSNRTKQLKSQESQNKARIQEKYDSKVKERKTENDKLVGYSGYAEGYSLE
                     IAETQAKIEALDAEIDKLNEELNTFTIYVDMGFNEPEPFEDIDPWAYYEVRIAWSEYR
                     EHKWSNKKVSQSFIRTPSGRHGVPGTYDFRFSVVMGPILTIKLFARSLVSFARVGLSV
                     VVGGFQLSCNVKVIPLFNMEGSSQLGFIKPKQATFYQSFLSARTNLGKTIRWNEDESL
                     PLTLTGNQGKKTYEILYASEREYKVLFPADYNFSLGSPSDFIYQDHKRNYYVEYDENQ
                     HLFAKGGAIANAGKVSVDVPIPKIKLTAFPKITDRPDSLSRERLGGTDQLSYAAINKL
                     VEEKQVTASTALQLHASQAFAKDINTSVTVGQSKASEAFINFGAYEITQVYPIEKRRE
                     LKFKPFFHAYVCKFMEELNQGGIDGLLQIQNQILSDIQKRDLGAGSSKIVNNFVMRYF
                     PNVPNVKEPYPLEEVDFSAEGAYSLYNWELFFHVPMLIANRLSQNQRFEEARRWYHFI
                     FNPTTNENLKLSARFWQVIPFRNTKQETLEALMMQLHRSAGDPVRKAFEDQITAWRDS
                     PFNPHLIARMRQQAYMKNVVMKYLDNLIAWADSLFRQDTMESINEATQLYILAAEMVG
                     KRPEKIPSRVKNIALNYAELEKGLDKFSNALVQLETLFPFYNIQPIPPGSPGVGPILN
                     NTAPSLYFCLPNNE"
BASE COUNT          974 a          882 c          811 g         1163 t
ORIGIN      
        1 tttcgttgtt gggcaagcaa aaatagagtg atggggcagt attattgaga atcggcccta
       61 cacctgggga acccggcggt atcggttgaa tattataaaa agggaatagc gtttccaact
      121 gcaccagtgc gttcgaaaac ttatccaggc ctttctccag ctcggcataa ttcagcgcga
      181 tattctttac acgggatgga attttttcag gccgtttacc gaccatctcg gccgccagaa
      241 tatagagctg agtcgcctca ttgatcgatt ccatcgtatc ctggcgaaaa agcgaatctg
      301 cccaggcaat cagattatca agatatttca tcaccacatt tttcatgtag gcttgttggc
      361 gcatgcgtgc aatcaaatgg ggattaaatg gagaatcgcg ccacgccgtg atctgatctt
      421 caaacgcctt gcggacggga tcaccagctg acctatgtag ctgcatcatt aatgcttcca
      481 aggtctcttg tttggtatta cggaaaggaa taacctgcca gaaccgtgcg gataatttca
      541 gattctcgtt ggtggttgga ttaaagatga agtgatacca acgcctggct tcctcgaaac
      601 gttgattttg gctcaagcga ttggcaatca gcatcggcac gtggaaaaat agttcccagt
      661 tatagaggct ataggcccct tcagcgctaa aatcaacctc ttccagtggg taaggttctt
      721 tgacattggg tacgttcggg aaatagcgca ttacaaaatt gtttactatt tttgacgaac
      781 ctgctccgag atcccgcttc tgaatatcac taagtatttg attctgtatc tgtaacaatc
      841 catcaatgcc gccttgattc aattcttcca taaacttaca cacgtaagca tggaaaaatg
      901 gtttgaactt caattctctt cttttttcaa ttgggtagac ttgagttatt tcatacgctc
      961 cgaaattaat aaacgcctct gatgccttgg attgacctac tgtgacagaa gtatttatat
     1021 ctttggcaaa cgcttgtgat gcgtgcaatt gcaaggcagt cgaagccgtg acttgttttt
     1081 cttctaccag cttattaatc gcagcgtatg aaagctgatc agtgcctcct aatcgctcac
     1141 gtgatagcga atcaggccga tctgttatct tgggaaaagc ggtaagttta atttttggaa
     1201 ttggaacatc aaccgaaact ttcccagcat tcgctattgc cccccccttc gcaaagaggt
     1261 gctgattttc atcatattcg acgtagtaat tgcgcttatg atcctggtag ataaagtcgg
     1321 aaggagaacc gagggagaaa ttgtagtcgg ctggaaagag cactttgtat tcccgttcac
     1381 tagcatagag tatttcatac gttttttttc cttggttgcc ggttaatgtc aacggcagtg
     1441 attcgtcttc attccaacga atcgttttac ctaaattagt tcttgctgaa agaaagctct
     1501 ggtaaaaagt tgcttgttta ggcttaatga atccaagttg agaggatcct tccatattaa
     1561 acagtggaat gaccttgaca ttacaactca attgaaatcc gcccacgact acgcttaaac
     1621 ccactcgggc aaaactaaca agtgaccgcg caaatagttt tatcgttaag attggtccca
     1681 taacgacaga gaatctaaag tcatacgtgc ccggcacacc atgacgacca ctcggtgttc
     1741 ggataaatga ttggcttact ttcttatttg accatttatg ctcgcggtat tcgctccacg
     1801 caattctaac ttcatagtaa gcccaaggat caatgtcttc aaatggttct ggctcattga
     1861 accccatgtc aacataaatg gtaaaagtat taagttcttc attcaattta tcaatttctg
     1921 catctaatgc ttctattttt gcctgggttt ctgcgatctc aagactataa ccttctgcat
     1981 atccactata acctactaac ttgtcattct cagttttacg ctctttcacc ttagaatcat
     2041 atttctcttg aatccttgcc ttgttttgag attcttgaga ttttaactgt ttcgttctat
     2101 tggattgagc ttcactgtcc gcttttcgtt ttttccattg tttatgtggt tctctgtctc
     2161 gttcaatttt ttctttatcc gatttctccg taaaaatcgg ccagaacaga tacaaacgcc
     2221 ggttccacac gacgggcata agatgcactc ctgccgaatc accatcttca ttgccttgaa
     2281 tatcgagtgg tacatgctcc caggctgtcc aaacctgagt ttttttatcc agtttgcggt
     2341 agaagtactt cggcggggta ttgaatgttc gtccgaaaac atgcatctct ttcgcatcat
     2401 gatcctcgta aatgccgcag atatcgaggc gcgatacttc gtccagtttc tccagataat
     2461 gcataaactt ctgctcggca atttcgtcgg tcaattcacc ttgcaataac tcactctcca
     2521 gatctttgaa gaaagggctt ttattgtctc gcaactccgg ctcgatccaa ttttccggat
     2581 acaagaatac tttccggttt gcctcccaga cgcgatagag cctcatccac tcccagtgtt
     2641 ttttattaat caattcgggc ggaattttgt gactttcttc cattttgtta ctttcttcca
     2701 gattgagcag gcagcgctgc acaaacaact gcaccaaggc gatcgcctgt ttcaagcgcg
     2761 aagtgagcat gcaagcgccc atctctacgt cgatcaagaa ataagcatag agatcattgg
     2821 cacttttcag tccaattttt tggatttcgg atttctgcaa cagataggcc acgagcgcat
     2881 cgcgcagatg catgcgcaat cgattatgaa cggcggttga tacttccacc caggcatctt
     2941 cgctgtattt tgctttcagc gttcgcttga tatcctgcga ctgttcatag gatacgctat
     3001 cggttgtcca tgactcgagt tcttctatcg agaccccgat tttctcgctg agcgccaatt
     3061 ttctctgaag attaatcaaa gtgatctcat ttagaaagtc agcagacgta tgctgagcaa
     3121 cgaaatacgc cacgttgatt ttgtcccagg cggtaacatc cacaatggct tgggtaatat
     3181 caccgccttt ttttgcggtt tcaaacagtg atagtaagct gctctctgcg gggaacaagc
     3241 tgctctcggc agggaataag ctgctcttgg caggcggcag tgaatcacgc agtgcagcaa
     3301 aatcggcgat gcgcagccac aatttaaagt cgagcgcttt ccagttgaag ttgctgaaat
     3361 tcggattatt ttcaaaatgc gtcaattctt tgcctgaaag ttttaatttc ccgactagcc
     3421 aggtgaatcg atgcatcaga atatactgct cggcatacgt tttgtcattt gctggagcgg
     3481 tttctacatt gaggcaagtt tgcaagatga ccgtttcttg cagcatcagt ttaacgatat
     3541 ccgcttccac tttgaaagca gcgatgagat gttgctgcac aaaggtttcg cgcaaaacgg
     3601 gcagcagtct tttctcgata tgcgcccagt acatcgtcaa gcgcttagga atatcggtcg
     3661 ccatttcgag cgcagtgaca tccgctgcgg aaagataatc ctttaaaaca gttttgtaag
     3721 ggtccaaaac tttgagtgtg cttaggtcac caaaaggggc tttggtattt gatccatccg
     3781 gatcgccgtt gactttgctc aaaatcgcga tgactttgct gatttcatcg
//
LOCUS       DAORVT010000091         3829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_19026_length_3829_cov_4.268415, whole genome shotgun sequence.
ACCESSION   DAORVT010000091 DAORVT010000000
VERSION     DAORVT010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19026_length_3829_cov_4.268415"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="PK389_02580"
     CDS             complement(<1..829)
                     /locus_tag="PK389_02580"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HQY22630.1"
                     /translation="MIKSLRSVLIIVAVGLALLGLTVYTVYESDRAIIARLGELKKDA
                     DGQVKIYGPGLHCKIPMIDVVHIFDMRLSMTEVPSERIVTKEKEVLLVDLFVQWRISD
                     FALFYNTTYGMSDGLGGRDRAEQLLRQNVKGVLHSEFGQRTLQNVVSGDRKELMEKLR
                     KRANDNVTPYGMEIVDVRVNRVEYPPEVNDKVFARMSSERKRVAVAYRASGESKAAII
                     RADADRMARVMIAEAQKESERLRGEGDAKAAQVYADSYNQSPDFYRFYRSLEAYKNVF
                     "
     gene            complement(826..2001)
                     /gene="hflK"
                     /locus_tag="PK389_02585"
     CDS             complement(826..2001)
                     /gene="hflK"
                     /locus_tag="PK389_02585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HQY22631.1"
                     /translation="MIKKSYPMAWNKPGDKDNNNPWGRKNGEQGPPDLDQIFRHFNNK
                     IKQLFSGKPSSGFTTNGSGQEFQFFAGIGLLVILVLYIISGIYIVDPPERAVVTRFGK
                     YVRTEDPGPHWLPPLIESKEVVNVEQVATSDHNGLMLTRDGNIVSVGVAVQYRVGDDN
                     DDVRAYLFNVVNPVRSLDQSAESALRQVLGQSTMDEVLTSKRSEIATAIKEQMIETLK
                     NYHTGLAVLDVVMQFAKAPDEVRAAFDEVIKASADEERLVNQARGYENEVLPRAKGNA
                     ERLRNEALGYKQETILIAEGNVQRFNLVLPQYLKAPKVMQTRMYLDAMEQILDKTSKV
                     VVDSGLGNNMIYLPLDRLIAGSVSKEPANADESTSGFSANNAKEQMPVASQFKEKNP"
     gene            complement(2025..3311)
                     /gene="hflX"
                     /locus_tag="PK389_02590"
     CDS             complement(2025..3311)
                     /gene="hflX"
                     /locus_tag="PK389_02590"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020584270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HQY22632.1"
                     /translation="MIERPEGGERVVLVHLHLRSAHIISDAEEFRELALAAGAEIVEI
                     ITTTRDAPDAKFCIGSGKVQELEALVAAHSVELVLFDHALTPAQERNLERALKCRVID
                     RTGLILDIFAQRARTYEGKLQVELAQLKHHASRLVRGWTHLERQKGGIGLRGPGETQL
                     EVDRRLIRGRIKYIQARLEKVRKQRDQGRQSRQKASLPTVSLVGYTNVGKSTLFNALT
                     GADVFVADQLFATLDPILRTIELPFVGKVICADTVGFIRQLPHGLVEAFRATLEETKD
                     ADLLLHVIDSQDESREAHIADVERVLEEIGALAVPRLEVYNKIDLAKALMPQVLRDAE
                     GAVQRVFMSATTSAGLGLLKEAITERLAYEMVSGEIGLSSNKSEIRALLFEMGAVVSE
                     SLDTEGHWCLRIRVPRLKWDQLCRQDPELETCWQKT"
     gene            complement(3312..3608)
                     /gene="hfq"
                     /locus_tag="PK389_02595"
     CDS             complement(3312..3608)
                     /gene="hfq"
                     /locus_tag="PK389_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HQY22633.1"
                     /translation="MSKGQMLQDPFLNALRKERVPVSIYLVNGIKLQGHVESFDQFVV
                     LLKNSVSQMVYKHAISTVVPTRNVKLAHGAHADENDETEIEDEEFASEEELGNM"
     gene            complement(3657..>3829)
                     /locus_tag="PK389_02600"
     CDS             complement(3657..>3829)
                     /locus_tag="PK389_02600"
                     /inference="COORDINATES: protein motif:HMM:NF013845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HQY22634.1"
                     /translation="KYLEGEYTSDEIIYRAIVATRQLAKRQLTWLRSLSDITWLSNTR
                     LASLSTVIHCVS"
BASE COUNT         1053 a         1001 c          661 g         1114 t
ORIGIN      
        1 taaagacatt tttataagcc tctaggcttc tataaaatcg gtaaaagtcg ggcgactgat
       61 tataactatc cgcataaact tgtgccgctt ttgcatcacc ttctccacgc aaacgctccg
      121 attctttttg cgcttccgca atcattaccc ttgccattct gtcggcatct gcacgaataa
      181 tcgccgcttt tgattctcca ctggcacgat atgcaaccgc aacacgttta cgctcactgc
      241 tcattcgcgc aaaaactttg tcgttcactt ctggtggata ctctacacga ttaacacgca
      301 catcaacaat ttccatacca tagggtgtga cattatcatt cgcacgtttt cttaattttt
      361 ccattaactc tttacgatca ccagaaacca cattctgtaa agtgcgttgt ccaaattccg
      421 aatgcaaaac acctttcaca ttttggcgca gtaattgctc tgcccgatca cgtccgccta
      481 atccatccga cataccatac gttgtgttat aaaacagcgc aaaatcactg atacgccatt
      541 gcacaaacaa atcaaccaat aacacttctt tttctttagt cacgatacgt tcggagggca
      601 cttctgtcat acttaaacgc atatcaaaaa tatgtacgac atcgatcatc ggaattttac
      661 aatggagtcc tggtccataa atttttactt gtccgtccgc gtcttttttg agctccccta
      721 atcgcgcaat aatcgcgcga tcggactcat aaacggtata gactgttaac ccgagtaatg
      781 ccaaaccaac ggcaacaata atcaacacag agcgcaatga cttaatcatg ggtttttctc
      841 cttaaattgc gacgccacgg gcatttgctc ttttgcatta ttcgccgaga aaccactggt
      901 agattcatct gcatttgccg gttccttaga aacactaccc gctattaaac gatctaaggg
      961 taaataaatc atattattcc ccagccctga atcgacaacg actttgctgg ttttatctaa
     1021 gatttgttcc atcgcatcta aatacatacg cgtctgcata actttaggcg cttttaaata
     1081 ctgcggtaag accaaattaa atcgctgtac atttccttct gcaattaaaa tggtctcttg
     1141 tttgtaaccc aatgcctcat ttcgcaaacg ctctgcatta cctttcgctc tgggcaatac
     1201 ctcattttcg taaccccgag cctgattgac taatcgctct tcatctgcgg aagctttaat
     1261 cacttcatca aatgccgcac ggacttcatc aggtgctttc gcaaattgca taaccacatc
     1321 taataccgca agacccgtat gataattctt tagtgtttct atcatttgtt ctttaatagc
     1381 tgtcgcaatt tcagatcgtt ttgaggttaa cacctcatcc attgtagatt gtcctaatac
     1441 ctggcgtaat gcactttctg cggattgatc aagactccga actggattga cgacattaaa
     1501 caaataagca cgtacatcat cattatcgtc acccacacga tattgaacgg caacaccaac
     1561 cgaaacaata ttaccatcac gtgttaacat caaaccatta tgatcactcg ttgcaacttg
     1621 ctctacatta accacttcct tcgactcaat taacggagga agccaatgcg gacccggatc
     1681 ttcggtgcgc acatattttc caaaccgcgt aacgaccgca cgttcaggtg gatcaacaat
     1741 atatatgcca cttataatat acaaaaccag tataaccagc agcccgatac ccgcaaaaaa
     1801 ttgaaattct tgcccactcc cattcgtcgt aaaacctgaa ctgggtttac cgctaaatag
     1861 ctgttttatt ttgttattga agtgtcgaaa aatttgatcg agatcaggcg gaccttgctc
     1921 accatttttt cgaccccagg gattattgtt atctttatcg cccggtttat tccaagccat
     1981 agggtaagac tttttgatca tgtttacact tcctatttcc atgattatgt tttctgccaa
     2041 caggtttcta attcaggatc ttgtctacac aattgatccc acttaagtcg cggaactcga
     2101 atccgcaagc accaatggcc ttctgtatcg agggattcac tgacaaccgc tcccatctca
     2161 aataataacg ctcttatttc ggacttgtta gatgacaaac ctatttcgcc actcaccatt
     2221 tcgtacgcca aacgctctgt gattgcctct ttcaacaaac caagtccagc gctcgttgtt
     2281 gccgacataa acacgcgttg aacagcccct tctgcatctc gtaacacctg aggcattaag
     2341 gctttggcca aatcaatttt attgtaaact tctaagcgcg gcaccgccag agccccaatc
     2401 tcctcaagga ccctttcaac atctgcaata tgcgcctcac gcgattcatc ttggctatcg
     2461 atgacatgca gcaaaagatc agcatctttc gtttcttcga gcgttgccct aaatgcctct
     2521 actaacccgt gtggcaattg acgaataaag ccaacggtgt cggcacaaat cactttgccc
     2581 acaaaaggca attctatcgt acgcaatata ggatcgagcg ttgcaaataa ttgatccgca
     2641 acaaatacgt ctgcgccggt taatgcatta aataaggttg atttcccaac attggtatat
     2701 ccaaccaacg acaccgttgg caatgatgct ttttgccgcg attgccgtcc ctgatctcgc
     2761 tgtttgcgaa ctttttctaa ccttgcttga atatatttga tacgccctcg aattaaacga
     2821 cgatcaactt ctaactgggt ctcaccaggc cctctcagac caatgccgcc tttttgtcgc
     2881 tctaaatgcg tccagccacg caccaaacga gaggcatggt gcttaagctg agccaactca
     2941 acttgtaact taccttcgta agtacgtgca cgttgcgcaa aaatatcgag aataaggcct
     3001 gtgcgatcga tgacacgaca ttttaaagca cgttccaaat tacgttcttg tgcaggcgtt
     3061 aacgcatgat caaacaagac gagttcgaca gaatgtgcag caacaagggc ttctaattct
     3121 tgcactttac cactgccaat acaaaatttg gcatctggtg catcgcgcgt ggtggtgata
     3181 atttctacaa tctcagcgcc agcggccagt gcaagttccc gaaattcttc ggcatccgaa
     3241 atgatatgtg cacttcgcaa atggagatga actaaaacga ctcgctcacc tccctctggt
     3301 cgttcaatca actacatatt ccccagctct tcttcgctcg caaattcttc atcttcgatt
     3361 tcggtttcgt cattttcgtc agcgtgtgca ccgtgagcca atttaacatt acgcgtcgga
     3421 acaaccgttg atatcgcatg cttatagacc atttggctaa cgctgttctt taacaacaca
     3481 acaaattgat cgaaagattc tacatgcccc tgcagcttaa ttccatttac taaataaatt
     3541 gacaccggta cccgctcttt acgcaatgcg tttaggaatg ggtcttgtaa catttggcct
     3601 ttagacatat ttattttctc catgatgtta ttgtggctat tccacctaaa ctccccttaa
     3661 gaaacgcagt gtatcacggt cgacaaagaa gctaaacggg tatttgagag ccaagtgata
     3721 tcagataagc ttctcaacca agttaattgt cgtttcgcca attgccgcgt tgccacaatc
     3781 gccctataaa taatctcatc agaagtgtat tctccttcta aatatttcc
//
LOCUS       DAORVT010000092         3813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_19186_length_3813_cov_3.481107, whole genome shotgun sequence.
ACCESSION   DAORVT010000092 DAORVT010000000
VERSION     DAORVT010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19186_length_3813_cov_3.481107"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PK389_02605"
     CDS             complement(<1..377)
                     /locus_tag="PK389_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22635.1"
                     /translation="MESLVLEPTSMAQWHSLVKEARQASSVSLSEELESYLIFLLMRF
                     INHPEIANTLFALDFLQNIKQLKTENQNIIRNIGDKCLLYSGLFPGRAKRQHVEISYY
                     VDLGRRSYFALSVSRQHILSPLFG"
     gene            569..2389
                     /gene="typA"
                     /locus_tag="PK389_02610"
     CDS             569..2389
                     /gene="typA"
                     /locus_tag="PK389_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018138979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HQY22636.1"
                     /translation="MLEKLRNIAIIAHVDHGKTTLVDKLLRQTGTLGEERSGGVERIM
                     DSNDLERERGITILAKNTAINWRDYRINIVDTPGHADFGGEVERILSMVDCVLLLVDA
                     VDGPMPQTRFVTQKAFKQGLNPIVVINKIDRPGARPDWVVDQIFDLFDKLGATEKQLD
                     FPVVYASALQGYATLDLNTPSIDMTPLLETIVKYVSPPPATSEGPFQMQISALAYSNY
                     VGVIGTGRISRGQIRTNTQVSIVDRFGKVRTGRVMQILAFKGLERIEVTEAFAGDIVA
                     ISGITDLTISDTVCAKDVPEMLPPLNVDEPTISMTFQINDSPFVGRDGKFVTSRQLRE
                     RLAQEVIHNIALRVEETSDADKFKVSGRGELHLGILIETMRREGYELAVSRPEVILHD
                     VDGVTHEPFEQLVVDLEETHQGTIMQQLGERRGLLNDMISDGRGRVRLDYTIPARGLI
                     GFYTEFQTLTSGSGILTHVFDHYGPADKSAVAKRNVGVLISNAAGKATAYSLWNLQPR
                     GRLFIGAQTEVYEGMIVGIHTRDNDLVVNVTTEKKLTNMRASGTDEKAVLVTPIRLTL
                     EYALEFIEDDELVEITPKHIRLRKKFLKEPDRKRASKGSA"
     gene            complement(2568..3302)
                     /gene="tatC"
                     /locus_tag="PK389_02615"
     CDS             complement(2568..3302)
                     /gene="tatC"
                     /locus_tag="PK389_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HQY22637.1"
                     /translation="MDSYIPHLIECRTRIIRSGLIVLCLFAALFCIDEHLYAWIAKPL
                     LSQLPAGGSIIATAVTTPFMVPMKLALLLSFFISIPYLLYEIWSFIAPGLYLKEKKRI
                     LPFLITSTLLFYAGVGFAYCIICPMALGFFAKCAPVGVLVMTDIQSYLDFVLSVLFAG
                     GIAFQAPVITLVLLQSGLVSISQMEYCRPYIIIAAFIVGMILTPPDVISQILLALPMW
                     WLFEAGLLLAKFLKGRTQPSTPTVST"
     gene            complement(3278..3409)
                     /locus_tag="PK389_02620"
     CDS             complement(3278..3409)
                     /locus_tag="PK389_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HQY22638.1"
                     /translation="MGSLLVILIVALVVIKPERLPEITFLLGRLTARFKLWIHTFRI"
     gene            complement(3412..3597)
                     /gene="tatA"
                     /locus_tag="PK389_02625"
     CDS             complement(3412..3597)
                     /gene="tatA"
                     /locus_tag="PK389_02625"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HQY22639.1"
                     /translation="MGFGGISMGSLLLIFLIVLVLFGTKKLRTMGEDLGQAVRSFRKG
                     LQDPHDKKQSDSDKNPL"
     gene            complement(3619..>3813)
                     /locus_tag="PK389_02630"
     CDS             complement(3619..>3813)
                     /locus_tag="PK389_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008290880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HQY22640.1"
                     /translation="IKSLAEISEKEANLIAHMLLVMPSLATREGLKGFRTIINTGKEG
                     GQVIDHLHFHLLGGDHLPGM"
BASE COUNT         1165 a          744 c          855 g         1049 t
ORIGIN      
        1 ccaaagagcg gtgacaaaat atgttggcgt gaaacggaga gtgcaaaata tgagcgcctg
       61 ccaagatcga cataataact aatttctaca tgttgtcgtt ttgctcgacc cgggaaaaga
      121 cctgaataca gcaaacattt atcaccaatg tttcgaatga tgttttgatt ttcagttttt
      181 aattgtttaa tgttttgtag gaaatctaat gcgaaaagag tattggctat ttccgggtga
      241 tttataaaac gcatcaagag aaaaatcaaa tagctttcga gttcttcgct taaagaaaca
      301 gacgaggcct gacgtgcttc ttttaccaag ctgtgccact gcgccattga agtgggttct
      361 agaaccaagc tttccatgaa gagctcctct attctaccgt gcggatacac gaaaaaagtt
      421 agatcttctc aatcaatgag acctttttct ttcagaatag ttcaattgtc caataattga
      481 ttcatttaac gaaatactgt acaatgctaa ctcagacttt tagggtctaa tccgttcggt
      541 cgtttcatta aaaagagagt agttttgtat gcttgagaag ttaagaaata tcgccattat
      601 tgcgcacgtt gatcatggta aaacaacatt ggttgataaa ttattgcgac agacaggtac
      661 gcttggagaa gagcgctctg ggggtgtcga acgcattatg gattctaatg atttggaacg
      721 cgaacggggg attactatac tggctaaaaa cacagccatc aattggcgtg actatcgcat
      781 taatattgtg gatacaccag gacacgcaga tttcggggga gaggtcgaac ggatcttatc
      841 gatggtagat tgcgtgcttc tattggtcga tgctgtagat ggccccatgc cacaaacgcg
      901 atttgtaact caaaaagcat tcaagcaggg cttgaaccca attgttgtga ttaacaagat
      961 agatagaccg ggtgcacgac cggattgggt ggttgatcag atctttgatc tctttgataa
     1021 attgggcgca acagaaaaac agttagattt tcctgtggtt tatgcttctg cattacaagg
     1081 ctatgcgact ttagatttga atacgcctag tattgatatg acaccgctat tagaaaccat
     1141 tgttaaatac gtatctccac cgccagcaac atccgaaggt ccgttccaaa tgcaaattag
     1201 tgctttggcc tattcaaatt atgtgggtgt gattgggact ggtcgtatca gtcggggaca
     1261 gatccgaaca aatacccaag tatctattgt tgatcgtttt ggaaaggttc gcactggtcg
     1321 tgtcatgcaa attttggctt ttaaaggttt agagcgcata gaagtaacag aagcgtttgc
     1381 cggtgatatt gttgcgattt cgggcattac ggatctcact atttcagata ccgtgtgtgc
     1441 caaagatgtg cctgagatgt taccgccttt aaacgttgac gaaccgacca tcagtatgac
     1501 ttttcaaatt aatgactctc cttttgtggg tcgcgatgga aaatttgtaa cgagtcgaca
     1561 attgcgcgag cgccttgctc aagaagtgat ccacaatatt gccttgcgcg ttgaagaaac
     1621 cagcgatgcg gataaattta aagtatcggg tcgcggtgaa ttacatttgg gtattttaat
     1681 tgaaacgatg cgccgtgaag gatatgaatt ggcggtatct agacccgaag tgattttgca
     1741 tgatgtagac ggtgtgacac acgaaccctt tgaacaatta gtcgtggatc ttgaagaaac
     1801 gcatcaaggc accattatgc aacaattggg agaacgaaga ggattattaa atgacatgat
     1861 ttctgatggt agaggtcgtg ttcgtttaga ttatacaatc ccggcgcgtg ggctgattgg
     1921 cttttatacc gaatttcaaa ccttaacctc gggttcgggt attttaacgc atgttttcga
     1981 tcactatggt cctgcagata aatcggcggt cgcgaaacgt aatgtagggg tgttaatttc
     2041 taatgcagca ggaaaagcga ctgcttactc gctgtggaat ttgcaaccgc gaggtcgcct
     2101 ttttattggc gctcagaccg aagtgtacga aggaatgatc gtcggaatac acacgcgaga
     2161 taatgattta gtcgttaacg tcacgaccga gaaaaagctc acgaatatgc gagcttcggg
     2221 tacagatgaa aaagcagttt tagtaactcc gattcggtta accctagaat atgcattaga
     2281 atttatcgaa gatgacgaac ttgttgagat cacacctaaa catattcgtc tacgtaagaa
     2341 gttcttaaaa gagccagatc gaaaaagggc aagtaaaggg tctgcttaaa aaaggtgccc
     2401 aggtgaggaa cctgggctaa aaccgaataa tgtcggttgg agattgacaa gtccatattg
     2461 cctcagttgc tatatcctgc ctagaggcaa tcgccctttg tttttgtagg atattgtcca
     2521 tacgtgcagg gcggataggg tgggagacct ctacatgaga catttatcta ggtagacact
     2581 gtaggagtag atggctgtgt tcgccctttt aaaaatttcg ctaacaagag cccagcctca
     2641 aacagccacc acatgggcaa ggcaagcaaa atttgagaga taacatctgg gggtgttaag
     2701 atcatgccta caataaatgc agcaatgatt atatagggtc gacaatattc catttgcgaa
     2761 atgctgacta aacccgattg taataaaacc aatgtaataa caggcgcttg aaaagcaatg
     2821 cctcctgcga aaagcacgct taaaacgaaa tccagataag attgtatgtc tgtcattact
     2881 aaaacgccca caggcgcgca ctttgcaaaa aatcccaatg ccatggggca aataatacaa
     2941 taagcgaacc cgacacctgc ataaaataag agtgtactgg tgattaaaaa aggtaagatc
     3001 cttttttttt ctttaagata taaccctggc gcaataaaag accagatttc atatagaaga
     3061 taaggaatgc tgataaaaaa agaaagaagc agcgcaagtt tcatcggcac cataaagggc
     3121 gtggtaaccg ctgttgcgat aatgctgcca cccgcgggca gttgactcaa taagggttta
     3181 gcaatccagg cgtagagatg ttcatcaatg cagaacaatg ctgcaaacaa acacaaaaca
     3241 atcaaaccgc ttcgaataat gcgtgttcga cattctatta aatgcggaat gtatgaatcc
     3301 ataacttaaa ccgagccgtt aaacgtccaa gcagaaatgt gatttcgggt aatcgttcgg
     3361 gtttgatcac aactaatgcg acgattaaaa taactaagag tgatcccata ttcatagagg
     3421 atttttgtcg gaatcacttt gctttttatc atgaggatct tgtaatcctt ttcgaaaact
     3481 ccgtacggct tgtccgagat cttcgcccat cgttcgtaat tttttagttc caaaaagcac
     3541 taaaacgatt aaaaagatta agagcaaaga gcccatactg atgccaccaa atcccatact
     3601 gttctcctgt gcgaaaaatt acatcccggg aagatgatcc ccgcccaata aatgaaaatg
     3661 aagatggtcg atcacttgtc caccctcttt acccgtgtta ataatagttc gaaatccttt
     3721 taatccttct cttgttgcaa gcgagggcat aaccagcagc atatgcgcaa ttaagttagc
     3781 ctctttttca gaaatctcag ctaaactttt aat
//
LOCUS       DAORVT010000093         3795 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_19340_length_3795_cov_4.101337, whole genome shotgun sequence.
ACCESSION   DAORVT010000093 DAORVT010000000
VERSION     DAORVT010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19340_length_3795_cov_4.101337"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PK389_02635"
     CDS             complement(<1..428)
                     /locus_tag="PK389_02635"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HQY22641.1"
                     /translation="MKFTKYLTLSNRKVIIIGFGSIGPAALLLILRHIDINPAEIEII
                     SDSLKNQDIAENHGVKFSHFKLMPENYKKYLVSKLTQDDILINLSVEISSIDLIKLCQ
                     EMGVLYLDTSNEAWPSNITGVRTGTYERRQKMQQETNHFST"
     gene            complement(425..1954)
                     /locus_tag="PK389_02640"
     CDS             complement(425..1954)
                     /locus_tag="PK389_02640"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HQY22642.1"
                     /translation="MKEYKIHLALFPVFLLLIFVYVPTFAQSKLTNEELCYLPATELI
                     RLYHAKKLSPVDVLKAQIERIESQDHAFNSVTFKHYSEALTQAKESERRYRNGNPRLL
                     EGITCALKDDVEVKGWRMTMGSLIRKNVTQSKKDSALTTLLREAGVVMHVQTNVPEYY
                     CNLVTWNWLFGISRNPWNPMYTPGGSSGGSTAALAAGFSTLATGSDMGGSIRFPAAMT
                     GLYGFKPPYGRVPSSLIQYESLGPLARNFEDLNLFQQAISGPSPQMISSLRPKLIYHD
                     NYGDIRGWRIAYDPMDKWGIPIDKTVKKAMQQAIKNLEALGAIVEEVDLGFRARDFET
                     YARGLFSTSIGHFCFNEAEKNAHLITPYIKVLVNNYAKTGSSQHISTAEDWIEFQSTQ
                     IQKKVFLKGFKAIIMPTMCTPYVVADMGSTPENTIVTINDKPHSAETWIYAFTWPWNM
                     LGQYPVINVPIDLTPENIPIGMQIIGNTYDDLVIFQVASNWSKVAPAFYQSNGVKGNR
                     E"
     gene            complement(2028..3287)
                     /locus_tag="PK389_02645"
     CDS             complement(2028..3287)
                     /locus_tag="PK389_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HQY22643.1"
                     /translation="MAIHDYCGLDFGTSNSTIGVSTENNITMVPLDQGKPIIRSAIFF
                     NFEAGGCQFGQQGVTDYLSGVHGRLMMSLKSVLGSSLMDEDTSIQHRRISYREILGYF
                     LQHIKQQAETTLGHELTRIVLGRPVRFHDQDDVKDRLAQDTMESIARKLGFKTVLFQY
                     EPIAAALTYEQSITGEQLALIVDLGGGTSDFSVIRLRPGMENQDRLQDVLANNGIHIG
                     GTDFDTELSLKAVMPELGLGSEMRGMSGNIQVPNSYYYDLTTWHTINSLYTHRTRSEL
                     HTIKNFAFDPKRITRLINVIEKQAGHKILNEVEQGKCLLSNAEQVNLDFQFIEKEFFI
                     SIQQKMFENFIQNKIEKLVQVVLKTISDSGIKTSDIDSIFFTGGSTQIPMIRSAILEH
                     FPNAEIVQGDVFSSVGKGLIIDAKRKF"
     gene            complement(3405..>3795)
                     /locus_tag="PK389_02650"
     CDS             complement(3405..>3795)
                     /locus_tag="PK389_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22644.1"
                     /translation="LEQFSGLRIPNYELALETFLSTNKSKFLRVGVPDKDDHYANVLY
                     EKDIWNSLCLYQYNKNKKYIDCFFLCSKKDNVRILEAYVNEQSFIENWLAAVNQRLGG
                     CLDEDKHRSQFSNTVKSESLKTLLNTK"
BASE COUNT         1121 a          808 c          650 g         1216 t
ORIGIN      
        1 gttgaaaaat gatttgtttc ttgttgcatt ttctgtcgac gttcgtacgt tcctgtgcga
       61 acacctgtaa tgttagacgg ccatgcttca ttgctcgtgt ctaaatacaa aacacccatt
      121 tcctgacata acttaatcaa atctatggat gaaatttcta ctgacaaatt tattaaaata
      181 tcatcttgag ttaacttgga cacaagatat tttttataat tttcaggcat taatttaaaa
      241 tgtgaaaatt ttacaccgtg attttcagca atatcttgat tcttgagact gtctgaaata
      301 atttcaattt ctgcaggatt gatatcaata tgccttaaaa tcaataacaa ggcagcgggg
      361 cctattgatc caaatcctat gataatcact ttcctattgc ttagtgtaag atattttgta
      421 aatttcattc cctattccct ttgaccccat tgctttgata aaatgccggc gccactttag
      481 accaattaga cgcgacttga aaaataacta agtcatcata tgtgttgcct ataatttgca
      541 tgccaatcgg aatgttttca ggcgttaaat caataggcac atttataacc ggatattgcc
      601 ccaacatatt ccaaggccag gtaaatgcat aaatccacgt ctccgctgag tgcggtttat
      661 cattgattgt gacaattgta ttttcaggtg tgctacccat atcagcgaca acataagggg
      721 tacacatggt gggcattatt atcgctttaa acccttttaa aaatactttt ttttggattt
      781 gagtgctttg aaattctatc cagtcttccg ctgtggaaat atgttgtgac gaacctgttt
      841 ttgcataatt atttaccaat acttttatat acggcgttat taaatgtgca tttttttctg
      901 cttcattaaa acaaaaatgg cctatagagg ttgagaaaag tccacgcgca taagtttcaa
      961 aatcacgtgc tctaaatccc aaatcgacct cttctactat cgcgcccagc gcttccaggt
     1021 ttttgatcgc ctgctgcatg gctttcttga ctgtcttatc aattggaata ccccatttat
     1081 ccatcggatc ataggcaata cgccatccgc ggatatctcc ataattatcg tgatagatta
     1141 atttagggcg tagagatgaa atcatctgtg gactcggtcc tgatatcgct tgctggaata
     1201 gatttagatc ctcaaaattt ctggccaacg gcccaaggct ttcatattga atcagtgatg
     1261 acgggaccct cccatacggc ggtttaaatc catagagccc ggtcattgca gcaggaaagc
     1321 gtatagaccc acccatatca gaacctgttg caagtgtgct aaaccccgca gccaacgcag
     1381 cagtacttcc tcccgacgac cctcctggtg tatacattgg attccacgga tttctactga
     1441 tgccaaacaa ccaattccat gtcactaaat tacagtaata ttcgggaaca tttgtttgca
     1501 catgcataac gacgccagct tcccgaagca gcgtcgttaa tgcagaatct tttttagatt
     1561 gcgtgacatt ttttctgatt aatgacccca ttgtcattcg ccatccttta acctcgacgt
     1621 cgtccttaag cgcacaggtt ataccttcca gaagtcgagg gttaccatta cgatatcgtc
     1681 tttcggattc cttcgcttgt gtcaatgctt ctgagtagtg tttaaatgta acagaattaa
     1741 aagcatgatc ctgcgactct atgcgttcta tttgcgcttt taagacatct acaggtgaca
     1801 gtttttttgc gtgatacaac cgtattaatt ccgtcgcagg cagataacag agttcttcat
     1861 tcgttaattt tgattgtgca aatgtaggca catagacaaa gatgagcagt aaaaacactg
     1921 gaaatagagc taaatgtatt ttatattcct tcatagattt aactcccaaa ttaacacgta
     1981 acgcaaaaaa ttatacactg gtgattaaga aaaatatact aaaacgtcta aaatttacgt
     2041 tttgcatcaa taataagtcc ttttccaacg ctgctaaaca catccccctg aacaatctct
     2101 gcattaggga agtgttctaa tattgccgat cttatcatcg ggatttgcgt cgagccccca
     2161 gtgaaaaaaa ttgaatcaat atcactcgtt tttataccag aatcagaaat agtttttaat
     2221 acaacttgaa ccaatttctc aattttattt tgaataaaat tctcaaacat tttttgctgt
     2281 atgctgataa aaaattcttt ttcaataaat tgaaaatcaa gattaacttg ctccgcattg
     2341 cttaataagc attttccctg ttcaacttca ttgagaattt tatgcccagc ttgtttttct
     2401 attacattga tgagtcttgt gattcgtttg ggatcgaaag caaagttttt aatcgtatgc
     2461 aattcacttc gcgtacggtg cgtatagaga ctattgatcg tatgccatgt ggttaaatcg
     2521 tagtaatagc tattgggaac ctggatattg ccactcattc cacgcatttc actacccagc
     2581 cctagctctg gcataaccgc tttaaggctc aattcagtat caaaatcggt accaccaata
     2641 tgaatcccat tatttgccaa tacatcctgc aaacgatctt ggttttccat acctgggcgt
     2701 agccgtatca cggaaaaatc ggaagtacca ccacccaaat caacaatcaa ggcaagctgt
     2761 tcacctgtga tgctttgttc ataggttaat gccgctgcta tgggctcata ttgaaataag
     2821 accgttttaa aaccaagctt acgtgcaatg ctttccattg tatcttgcgc caaacgatct
     2881 ttgacatcat cttgatcatg aaatcgaaca ggtctaccca aaacaattcg cgttaattca
     2941 tgaccaagcg ttgtttcagc ttgttgcttg atatgctgta aaaaatagcc aagaatttct
     3001 cgataagaga tccgacggtg ctggatactc gtatcttcat ccattaaaga agatcccaac
     3061 acgcttttta aagacatcat taatcggcca tgtacgccag agaggtaatc cgtcactcct
     3121 tgctgaccaa attggcatcc gcccgcttca aaattaaaga aaatagccga tcgaataatg
     3181 ggtttccctt gatcaagggg taccatagta atattatttt ctgtagaaac ccctatcgtc
     3241 gagttgctgg tgccaaaatc aagaccacaa taatcgtgta tagccatcac gcgatcacct
     3301 accggatatt ttgaatgggc gtttacttta ccagtttata ggctattggg tcaaataccg
     3361 ggctcgcccc tatcataact caataaatat gcagcgcttt agaattattt ggtgtttaag
     3421 agcgttttca agctttcact tttaactgta ttagaaaatt gactacgatg tttatcttca
     3481 tctagacatc cccccaaacg ctgattcacg gcggcaagcc aattttcaat aaacgactgt
     3541 tcgttcacgt atgcttcaag aattcttaca ttatcctttt tggaacacag aaaaaaacag
     3601 tctatatatt ttttattttt attatattga tataaacata aagaattcca aatatctttt
     3661 tcatacagca cgttagcata gtggtcatct ttatccggaa cgcccactct taaaaattta
     3721 gacttattag tacttaaaaa agtttctaat gctaattcgt aattggggat ccgtagacct
     3781 gaaaactgtt ctaaa
//
LOCUS       DAORVT010000094         3792 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_19379_length_3792_cov_3.336366, whole genome shotgun sequence.
ACCESSION   DAORVT010000094 DAORVT010000000
VERSION     DAORVT010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19379_length_3792_cov_3.336366"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PK389_02655"
     CDS             complement(<1..626)
                     /locus_tag="PK389_02655"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HQY22645.1"
                     /translation="MNLSLASLRDELTKLKTSEEAILTQAQNPTFKTAELEYLIRLAS
                     ARLQTTRDLKAAIELLTLAQTKIQTLNDLHLSPLSEAIEKDILTLQQASTLNVEDLWL
                     KVSDIIDQTASISSQTLTANAQQTEATAQPQPQEKQENTSRLSILKQGFFESLESIKD
                     FIKIRHSTQRIEPLLSETQKNLIQENLRSLLEQIRLAILTTEDKIFQKA"
     gene            complement(749..1516)
                     /locus_tag="PK389_02660"
     CDS             complement(749..1516)
                     /locus_tag="PK389_02660"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HQY22646.1"
                     /translation="MRILVTRPANLAAGLSEKINAMGDIAELFPVIEIKKTPHQATLQ
                     AAIDTLDTQDIAIFVSQSAVQFGLPAIQSRWSTLPKMLWVAIGSGTAHALQNLGIHDV
                     LFPPAPPYESESLVAMPTFQSTQIHGKKISIFRGNGGRELLSETLRSRGALVQIIEVY
                     QRCLPTLDIPIVEKIKHWRHTPIHVIIATSADSLCNLVQLLGNAMNGLTEIPLVVVGA
                     RMQKLAKELNFKNPITATSADDASIINVLKTFKDTCV"
     gene            complement(1504..2451)
                     /gene="hemC"
                     /locus_tag="PK389_02665"
     CDS             complement(1504..2451)
                     /gene="hemC"
                     /locus_tag="PK389_02665"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HQY22647.1"
                     /translation="MQQTLRIVTRKSALALWQANFVKDKLQTLYPNLEITLIGITTEG
                     DQILDIPLTKMGGKGLFVKALEEHLLNRTADIAVHSIKDMPAALPDGLMLTAILERED
                     PSDAFISLQYPTLEALPPQATIGTSSLRRQAQLVALRHDLTIKMLRGNIDTRIQKLDN
                     NEYDAIILATAGLKRLGLAHRITQSFSTTDPTIKMLPAVGQGALGIECRISDKKIQHL
                     IMPLHHTPTAQCVLAERHMNALLGGNCQSPIAGLAIINDEGLLKLEGMVATPDSRTIL
                     KAQAIGNPEAMLIVGKQVAEQLLEQGAQDIITAATTCAS"
     gene            complement(2577..3515)
                     /locus_tag="PK389_02670"
     CDS             complement(2577..3515)
                     /locus_tag="PK389_02670"
                     /inference="COORDINATES: protein motif:HMM:NF015892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQY22648.1"
                     /translation="MRQNRPRNLCNLIVICLFFCILSGCASQNQLSSNKVNQTAYDIA
                     QLNHFQKQLIKTNPFMLTTFQKFSTSPSTSNTNHTINQTLTIYIEGDGRTWITRSKLS
                     NNPTPKNPLALKLAVLDNSANVAYLARPCQYTPHSLDSACNPLVWSDQRFSETVIQSM
                     NEAVENLKKSAKARQIHLVGFSGGGAIAILIAARRNDIASLRTVAGDLNPTALSQYHQ
                     TSPLTSSLDPNTRTVIQKLIHLPQQHFSGEKDKTVPPFIAEEFVARLRKLGSPCTSQT
                     TIKDATHHQGWEAIWPTLLDKPLGTSDGCQKNNVSF"
BASE COUNT          973 a          659 c          920 g         1240 t
ORIGIN      
        1 gctttttgaa aaattttatc ttcagtggtg agtatagcta aacgtatttg ttctaatagc
       61 gatcgcaagt tttcttgaat taagtttttt tgtgtttcgg acagtaaggg ttctatacgt
      121 tgtgtagaat gtcgaatctt tataaagtct ttaatgcttt ctaaactctc aaaaaaacct
      181 tgtttcaaaa tgctgaggcg agaggtgttt tcttgttttt cttgcggttg cggttgtgct
      241 gttgcttctg tttgctgagc gttggctgtc agggtttgag aggagattga agcagtttga
      301 tcgatgatgt cgctgacctt tagccataaa tcttctacat taagcgtcga ggcttgttgt
      361 agtgtaagga tatctttttc aatggcctct gataggggag ataagtgcag atcatttagg
      421 gtttgaattt ttgtttgtgc taaggtgagt agctcaatgg cggctttaag atcacgggtt
      481 gtttgtaggc gtgcgcttgc taaacggatt aaatattcta attctgctgt tttaaatgtg
      541 gggttttgtg cttgggttag aatggcttct tctgaggttt tgagctttgt cagctcatct
      601 ctaaggctcg ctaagcttag attcatcttg ttttctaaag ctgcgaggct tgcgtgagtg
      661 ctgctagaaa gagtttggta tctttcaaat tggaatgcac ttaatatccc gacactgatt
      721 gcaatgatta aactgaatat agccgttttc acacgcaggt atccttaaac gtttttaata
      781 cattaataat agacgcatcg tcggcgctcg tcgctgttat ggggttttta aaatttagtt
      841 ctttagctaa tttttgcatt cgcgctccca caacaaccag ggggatttct gttaatccat
      901 tcattgcatt tcccaagagt tgtacaagat tacaaagcga atccgcgctt gtggcgatga
      961 tgacatgaat gggagtatgg cgccaatgct taatcttttc aactatcggg atatcaagcg
     1021 ttggcaaaca acgttgatag acttctatta tttgcaccaa agcgccgcgt gatcttaagg
     1081 tttcgcttaa taactcacgt ccgccgttgc ctcgaaagat cgatattttt tttccgtgga
     1141 tttgggtgct ttgaaatgtg ggcatggcaa ctaaagattc ggattcgtaa gggggcgcag
     1201 gtggaaaaag gacatcatga atgcctaaat tctgtagtgc atgtgctgtg ccagatccta
     1261 tggcgaccca aagcattttt ggtagtgtag accaacgcga ttgaatggca ggtagtccaa
     1321 attgcactgc agattggctc acaaagattg caatatcttg tgtatctaag gtatcaatgg
     1381 cagcttgtaa tgtggcttga tgaggggttt ttttaatctc aataacgggg aaaagttctg
     1441 cgatgtcgcc catagcgttt attttttcag ataatccggc ggccaggttt gccgggcgtg
     1501 taactaagat gcgcatgtgg ttgccgcagt aataatatcc tgggcgcctt gctctaacaa
     1561 ttgttctgca acttgtttgc cgacgattaa catggcttct ggatttccga tagcctgtgc
     1621 ttttaagatt gttcgactat cgggtgttgc aaccatgcct tctaatttta acagtccttc
     1681 atcgttaata atagcgagtc ctgcgatagg tgattggcag ttaccgccca gcaatgcatt
     1741 catatggcgt tctgctaata cacattgagc agttggtgtg tgatgtaagg gcattattaa
     1801 atgttggatc tttttatcac tgatgcggca ttctatacct aatgcacctt gtcctacagc
     1861 gggcagcatc tttattgttg gatctgttgt cgaaaaagat tgggtaattc ggtgtgcaag
     1921 tccgagcctt ttaagaccgg cggttgctaa aataatcgca tcgtattcgt tgttgtccag
     1981 tttttgaata cgtgtatcaa tgttgccacg cagcatttta atcgttaaat cgtgtcgcaa
     2041 tgcaacgagc tgggcttgtc gccttaaact cgaagtgcca atcgttgctt gtggtggtag
     2101 tgcttcaagg gttggatatt gtaaagaaat aaaagcatcg ctgggatctt ctctttctag
     2161 aatagccgtt agcattaaac catccggtaa tgcagcaggc atatctttaa tcgaatgtac
     2221 ggcgatatct gcggtacggt ttaataagtg ttcttcaagc gcttttacaa acaagccttt
     2281 accccccatt ttggttaagg ggatatctaa aatttgatct ccttctgttg taatgccaat
     2341 cagcgtgatt tcgaggttgg gatagagtgt ttgtagttta tctttcacaa aattggcttg
     2401 ccataatgct aaagcacttt ttcgggtaac gatccttaag gtttgttgca tggggtctat
     2461 taaacgttat tttttatggg ttgtcaaaaa taatggtagt taaaaataag ggctgtcaaa
     2521 aaataatttt cctttttttc gcgagcttga actcgctgag ctcagacaaa cctcgctcaa
     2581 aaagaaacat tatttttttg acagccgtcc gaggttccca aaggcttatc taaaagggtt
     2641 ggccaaattg cttcccagcc ttgatgatgc gttgcatctt taatagtggt ttgtgatgtg
     2701 catgggcttc cgagttttcg caatcgggca acgaactctt cagcaataaa aggtggtaca
     2761 gttttgtctt tttcgcccga aaaatgttgt tgagggagat gaatgagttt ttgtatgaca
     2821 gtgcgggtgt tgggatctaa agaactcgtt aagggcgacg tttgatgata ttgacttaaa
     2881 gcagtgggat ttaaatcacc tgcaacggtt cttaatgagg caatatcatt cctacgcgca
     2941 gcaatcagta ttgcaatggc cccgccgcca gaaaagccaa cgaggtgaat ttgcctggct
     3001 tttgctgatt tttttaagtt ttctacggct tcgttcatgg attggataac ggtttcggaa
     3061 aaacgttgat cagaccagac aagcggattg caggcggaat ctagggagtg aggtgtgtat
     3121 tggcagggcc ttgcgagata agccacattg gcgctgttat ctaataccgc gagtttgagt
     3181 gcgagtggat ttttgggcgt tgggttgttg gacaattttg atcgcgtaat ccatgttcga
     3241 ccatcacctt ctatgtagat cgtgagtgtt tgattaattg tgtgattggt gtttgaggtt
     3301 gagggagatg ttgaaaattt ctgaaaagtg gtcagcatga agggattggt tttaatgagc
     3361 tgtttttgaa aatgattgag ttgagcgata tcgtaagctg tttgattaac tttgtttgat
     3421 gataattgat tttgtgatgc acagccggag agtatgcaaa agaataagca gataacaatt
     3481 aaattgcaca gattgcgtgg gcgattttgg cgcatctttc attcctcata taagctgaag
     3541 tagcaggata cagatttaaa caaaaagctg cacagtgtta aagacatgta gcaaaggaat
     3601 agcacaatac gcaggagcga cacgtgaact ttgaaagttt aataacatct tttaaaaaac
     3661 acatcattag tgggatggcc ttaagcttaa tttgcatagt acctgttgtt gaggcgactc
     3721 agtacgatgt tacaaatact aatgatgata caaatccagg ttcgttgcga tatgcaatta
     3781 cgcaagcaaa tg
//
LOCUS       DAORVT010000095         3779 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_19490_length_3779_cov_3.675349, whole genome shotgun sequence.
ACCESSION   DAORVT010000095 DAORVT010000000
VERSION     DAORVT010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19490_length_3779_cov_3.675349"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PK389_02675"
     CDS             <1..194
                     /locus_tag="PK389_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HQY22649.1"
                     /translation="PALQVIAIILMLAGGTEIAQLYITGRSPLLTDFFIDAAGGLSGI
                     TLIRLIGTNKNPVQSERNQ"
     gene            complement(233..1381)
                     /locus_tag="PK389_02680"
     CDS             complement(233..1381)
                     /locus_tag="PK389_02680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HQY22650.1"
                     /translation="MTEYINQLEVFPWNKNFETGIKIIDEQHQKLISLLNELAGALVR
                     GNQLEINYFFDELAKYAEFHFETEEVIWVECFGDDSWLSSHQLTHSSFLPKVVEIKEQ
                     ETNKPQNEIIESIILFLIRWLAFHILDNDKRMAFVVQNVQKGLSFEEAKIVSDKKMSG
                     SIRVLIETVMSMYDSLSSRTLVLMRENYERQKIEEELHKTNKQLRKVNLKLEALAMTD
                     QLTGLFNRRHFNNVFTRELKRACRDNIYLALIILDIDFFKKINDNYGHSKGDQVLVQV
                     SCKLKDLCQRPGDYAFRLGGEEFGVIAANLDYQGTVEFAEIIRKGIEELNILNGYSDG
                     ATKHLTVSIGSVTKVPDEADTVDNYMAIADARLYKAKELGRNQIVTGE"
     gene            complement(1475..2938)
                     /locus_tag="PK389_02685"
     CDS             complement(1475..2938)
                     /locus_tag="PK389_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HQY22651.1"
                     /translation="MYSTRRNCNQALLTICSLFFFSNAMVMAANAVEQLSTLKEQESV
                     AVTIYNDDLALIKDLRQVTLVNGDFNLAWRDVSAQMRPETALLRSITNPGSIEVLEQN
                     FNFDLLTPQSLLSKYIGQTVSIVKSNPATGVESKENAIVLAASNGVVLKMADRIETGM
                     PGRITFDNVPSNLRDRPTLVIGGDNSGKATQDLELSYLTGGISWKADYVAELNDKDDQ
                     LDLSSWVTLTNTSGTSYPNAKLQLVAGDINRVQREFAAPKAMRAKIMADAAAAPMREE
                     SLMEYHLYSLDRLTTIAENQTKQVALLNASGVPANKELVLAGSDYYYAASYGDLGQKM
                     KVDVFVQFDNKESARLGMPLPKGIIRVYKRDKTGNAQFAGEDYIDHTAKNETVRLKLG
                     KSFDVTADKKQTEFKQLSTGGINSINRFESAYEITLKNAKKEVATVVVQEPIPGDWKM
                     VDESQPHSKPASNTAMWKVDVPAEGTSTLKYRVHVKY"
     gene            complement(3029..3493)
                     /locus_tag="PK389_02690"
     CDS             complement(3029..3493)
                     /locus_tag="PK389_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HQY22652.1"
                     /translation="MQASEQRETNQNELQTVDQQTADLLCLHDSPLVRAMYLGAGFLA
                     LLLGALGAVLPVLPTTPFVLLAAACFARGSEYFHRKLLENRIAGPIIREWYIYHSIPR
                     RAKRWAYLLMALSFGSSILIVPELWQKIMLVMIGSILAFYIWRVPVREAKNY"
BASE COUNT         1019 a          846 c          751 g         1163 t
ORIGIN      
        1 aacccgccct ccaggtgata gctatcattc ttatgctggc cggcggcact gagattgcac
       61 aactctatat tactggccgg tcacccctac ttaccgattt ttttattgat gcggccggtg
      121 ggctgtcagg catcacgcta atcagattaa tcggtacgaa taaaaatcct gttcaatctg
      181 aacgcaatca atgattatgt ttctgtaaag atctgaccct ttcgatataa aattactcgc
      241 cggtaactat ttgattacga cccagttctt ttgccttata taagcgtgca tcagcgatag
      301 ccatataatt gtcaacggta tcagcttcat caggcacctt agtaactgag ccgatagaaa
      361 ccgtcaaatg tttagtagcg ccatcactgt aaccatttag aatgttcagc tcttctatgc
      421 cctttcttat gatttccgca aattccactg tcccttgata atccaggtta gccgctatta
      481 caccgaattc ttccccgcca aggcggaaag cataatcgcc gggacgttga caaagatctt
      541 ttaatttgca gcttacctgg actaacactt gatcaccttt gctgtgacca tagttatcat
      601 taattttttt gaagaaatcg atatccagta tgataagagc aagataaata ttgtcccggc
      661 aagccctttt aagctcacgt gtgaatacat tattaaaatg ccggcgatta aataaacctg
      721 ttagctgatc ggtcatggct agcgcctcta actttaaatt gaccttccgc aactgcttat
      781 tggttttatg aagttcttct tcaatctttt ggcgttcata gttttcacgc attaacacca
      841 gcgttcttga agataaacta tcgtacatac tcataacagt ctcgatgagt actctaattg
      901 aaccgctcat tttcttgtct gaaacgattt tggcttcttc gaaagacagc cctttttgga
      961 cattttgaac gacaaaagcc attcgtttat cattatccag gatatggaag gctagccaac
     1021 gaatgaggaa taaaataata ctttcaatga tctcattctg cggcttgtta gtttcctgct
     1081 ctttaatttc taccactttg ggtaagaacg atgagtgggt tagctgatga gatgatagcc
     1141 aggaatcatc accgaagcac tctacccaaa taacttcttc agtttcaaaa tgaaactcag
     1201 catattttgc caattcatca aaaaaataat ttatttctaa ttggttaccg cgtactaagg
     1261 ctcctgccaa ttcattcaaa agagaaatga gtttttgatg ctgctcatca attattttta
     1321 tccctgtttc aaaattttta ttccatggaa atacttcaag ttgatttatg tattcagtca
     1381 tataatctta ttatttagaa gtatcatatt aagtctgcaa gcaatttctc atcgcgacct
     1441 atttcactta ggaaaaagaa aggcgtacat gcattcagta cttcacatgc acgcgatatt
     1501 tgagagtgga tgtcccttcg gcagggacat ccactttcca catggctgtg ttactggcgg
     1561 gtttgctgtg cggctggctt tcatccacca ttttccaatc acctggaata ggttcttgca
     1621 caacaactgt ggccacttct tttttagcat ttttaagtgt gatttcatag gcgctttcaa
     1681 agcgattaat gctattgatg ccgccggtac ttaattgttt aaactcggtt tgttttttgt
     1741 ccgcggtcac atcaaacgat ttgcccagtt ttaagcgtac ggtctcgttt ttagctgtgt
     1801 ggtctatgta gtcttcaccg gcaaactgcg cattgccggt tttatcacgc ttgtaaacac
     1861 gtatgatgcc tttaggcagc ggcatgccta atcgagccga ttctttatta tcaaactgca
     1921 cgaatacatc tactttcatt ttttgcccta aatcaccata gctggctgca taatagtaat
     1981 cgctgcccgc cagcacaagt tctttgtttg caggcacacc ggaagcattg aggagtgcga
     2041 cttgtttggt ttggttttcg gcgatggttg ttaatcgatc caggctatat aaatgatatt
     2101 ccatcagtga ttcttcacgc attggtgccg cagcggcatc agccattatc ttcgctctca
     2161 ttgctttggg tgcggcaaat tctcgctgta cccgattaat atcgcccgcc actagttgta
     2221 gttttgcatt gggataactg gtgccgcttg tgttggtcaa tgttacccag ctggataaat
     2281 ccagttgatc atctttatca ttaagctctg cgacataatc cgctttccag gatatgccgc
     2341 ctgttaagta acttagctct aaatcttgcg tggctttgcc gctattgtcc ccgccaatca
     2401 ccagtgtagg gcgatcacgc aaattgcttg gtacattatc aaaggtaatg cgaccaggca
     2461 tgcctgtttc gatgcgatcg gccattttaa gcaccacacc gttgctggct gcgagtacga
     2521 tggcattttc tttgctttca acgccagtgg ctggattgct tttaactatt gacaccgttt
     2581 ggccaatata tttgctcaat aaactttgcg gagtgagcaa atcaaagtta aagttttgct
     2641 ccaatacttc aatactgccg ggattggtaa tgctgcgcag cagtgcagtt tctggccgca
     2701 tttgcgcact gacatcgcgc catgccaaat taaagtcacc attgactaag gtaacttgcc
     2761 gtaaatcctt gatcagtgct aagtcatcgt tgtaaatagt cactgcgacg gattcttgtt
     2821 ctttgagtgt acttaattgt tcaacggcat tggcagccat taccatggca ttactgaaaa
     2881 aaaataggct gcatatcgtt agcagtgctt ggttacagtt acgacgtgtt gagtacatgg
     2941 gattttccta taatagaaaa aataactagc aggatatttt ttgtgagaat ctaatcacga
     3001 atccacgttc cgggatcagg agctttcatt agtaattttt ggcctctcgc accggaacac
     3061 gccagatata gaaagccaat atactgccaa tcataaccag catgatcttt tgccatagct
     3121 cgggcacaat taaaatagag ctcccgaatg acaatgccat caataaataa gcccagcgtt
     3181 tggcgcgacg cgggatactg tgataaatgt accactcgcg aataattgga ccggctattc
     3241 gattttccag taatttacga tgaaaatact cagagccgcg agcaaaacag gcagcagcca
     3301 gaagcacaaa gggtgttgta ggcaataccg gcaatactgc tcctaacgca cccagcaaca
     3361 atgccaaaaa accggccccc agatacatgg ctcgcaccaa cggtgaatcg tgcaggcata
     3421 acagatcagc cgtttgctga tctactgtct gcagctcatt ctgatttgtt tcacgttgtt
     3481 cagatgcttg catagttttg atggattcag aattcgcagt gtaaaatcaa aagaattatg
     3541 gctatttagg tacttatgac caaacttatc tagaaacgat caattcgaac agcggctgat
     3601 aaagaacaga tgttaccagt tataataccg gagagttaat tgttagaatt aaccggattt
     3661 aacttttttc ttgaggaaac catgcataaa atgaaaacac tgtttaattt aagtatcatc
     3721 ctgctggcgt tggcgctgct tccccagaca gccttctctt ataaagaaca tgccgctgc
//
LOCUS       DAORVT010000096         3765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_19615_length_3765_cov_4.059030, whole genome shotgun sequence.
ACCESSION   DAORVT010000096 DAORVT010000000
VERSION     DAORVT010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19615_length_3765_cov_4.059030"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..975)
                     /gene="ftsY"
                     /locus_tag="PK389_02695"
                     /pseudo
     CDS             complement(61..975)
                     /gene="ftsY"
                     /locus_tag="PK389_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
     gene            1229..2578
                     /locus_tag="PK389_02700"
     CDS             1229..2578
                     /locus_tag="PK389_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005769952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQY22653.1"
                     /translation="MMYFKHLCLWLLFAAISGSALASETPPASNTHEYKLDNGLKLIV
                     REDHRSPIVVTQVWYKVGSSYEPNGITGISHALEHMMFRGTTNHPKDEFSRLIAIGGG
                     DQNAFTSSDFTAYFQELDAERLKLCFELEADRMANLTLPEDAFKQELKVIMEERRLRT
                     DDNPESLTRERLHAAAHISNPYHHWPIGWMNDIENLTVEDLRTWYKTWYGPNNAIVVV
                     VGDVNPEAVHQLAQTYFGPLKPITIPTLKPRKELVNLGKRQIQVSANATVPRLFMAYN
                     VPSIITASSPEEPYALLMLLMALDGGNSGRFSKDLIRDQSLITSVFSTYDPFSLHEAL
                     MEFSAIPTQNHTLAEVEAALLQQFERLKTSPISDAELNRVKVNAIAQHTYAKDSMSDQ
                     AIGLGSLESVGLSWKLADTFPEQIQTITAEDIQKVAQKYLTQERLTTAELIPLKSGT"
     gene            2583..>3765
                     /locus_tag="PK389_02705"
     CDS             2583..>3765
                     /locus_tag="PK389_02705"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQY22654.1"
                     /translation="MKNRYWRIVLFVFVLPIVLFFSFSKKVVKTESAATSVETSMIQQ
                     STTTPTPMPMPTPIPHTVAIENWSTTNGTRVYFVATEGIPIIDVQVNFDAGSARDAEK
                     PGLAALCLHLLDQGIPNASADLIAEKFEDSGAKFSVDVDRDRATISLRSLTDPKLLNP
                     VIALYVDLISNPTFPENNIETQRNQTLIGLQRNLQQPNAVASQAFFKALYKNHPYAEP
                     ACGTIEGVTLITKADLVQFHQQYFVAKNATITIVGGVSKEQATDIANQISEKLPEGNL
                     AAPIPNVEPLIEPTHVNISFPTEQTHVLTGQPCATENDPDYFPLLVGNNILGGGILTS
                     RLFNEVRNDRGLAYNVISAVLQLQKPAPFVIRLQTKNSQTQEAITVLQNTLVKFINEG
                     PT"
BASE COUNT         1107 a          882 c          712 g         1064 t
ORIGIN      
        1 cacctatagc cgtaccactg gccaataaaa tgcccccaaa cactttatct ttattgatca
       61 tcacatcgca tcgctagaaa ataacgcgtc aactaatgtt gctgcttcaa aggaacgaag
      121 atcatcaata ccctctccaa cccctataaa acgaatcgga attttaagcg ttttggcaat
      181 attaaaaatc ataccccctt tcgctgtgcc gtctagtttt gtgagggtta ttcccgtaac
      241 acccaacgct tcattaaatt tttgggcttg caccagtgca ttttgcccta tgccagcatc
      301 cagcactaac atgatttcgt gtggtgcttc ttcatctaat ttacgaatta ccctaaccac
      361 tttttttaat tcttccatga gggactcttg cgtatgtaat ctacccgccg tatcggcaat
      421 taatacgtca aacccttttg ctttcgccgt ctgaaaagca tcaaaaataa cagaagcgct
      481 atccgcaccc atatgttgag cgatcacggg tgtatggtta cgttcacccc acactttcag
      541 ttgttcgatt gctgcggcac gaaaagtgtc acctgcagcg agaataactt tttttccttc
      601 tgcttcaagt tttttagcca attttccaat ggttgtggtt ttaccggcac cattaatacc
      661 gaccattaat atcacaaaag gttttttatc acttaaaacc aaaggcactt cggcacttga
      721 taagagttga ttgagatctt gtttaagcgc taaagccaaa cctgcaggat cagaaagctc
      781 gcgacgatct aagcgctttg ttaaatcctg cataatttgt tcggtggaac tcacgccaat
      841 atccgctgat aacaaaaaag tttctaattc tgcgaggagt acgctgtcga tttctttttt
      901 gccgagaaac acctgcgcca gcccttctga aaaaaccgca cgcgtgcgac gtagcccgtc
      961 tttgaagcga gtgaacatgc tctttgcttc aggcaccttg ggttccggcg gaatttccgt
     1021 ttttttaccc cattttagaa aatccaacat tttatttgcc tatttgttga taacaccaaa
     1081 acacacagga tacagcagaa ggaatgttta taccaatggg cggaaaagac agaagaattg
     1141 aaaataatgt gtctgtgttc agacttgacc cattgccgcc tccttggtgt atactcccct
     1201 ttatagttaa tgttaacctc gggaataaat gatgtacttc aaacacctat gcttatggct
     1261 actcttcgcc gcgatcagtg gttctgctct tgcttctgaa acgccgcctg cttctaatac
     1321 ccatgaatac aagttagata atggtctaaa actaatcgtt cgagaagatc accgttcccc
     1381 cattgtggta acacaagttt ggtacaaggt gggtagtagt tatgaaccaa atggcatcac
     1441 gggcatttcg catgcactcg aacatatgat gtttcgcggc acgacaaacc accccaaaga
     1501 tgaattttct cgtttaattg cgattggtgg cggtgatcaa aatgcgttta cctcaagtga
     1561 ttttactgct tattttcaag agcttgatgc ggaacgttta aagctttgct ttgaactcga
     1621 agctgatcgc atggcaaatt taacactacc tgaagatgct tttaaacaag aacttaaggt
     1681 aataatggaa gaacgtcgat tgcgaacgga tgacaatcca gaatccttaa cccgagaacg
     1741 attgcatgca gccgcacata tcagcaatcc ttatcatcat tggcccatcg gctggatgaa
     1801 tgatatagag aatttaaccg ttgaagatct acggacttgg tataaaacat ggtatggccc
     1861 taataatgca attgttgttg tggtcggcga tgtaaacccc gaagccgtcc atcaattagc
     1921 acaaacctat ttcggtccct taaaacccat tacgatacca acactaaaac cgcgtaaaga
     1981 gctcgttaat cttggaaaac gtcaaattca agtgagcgcc aatgcaaccg ttccacgatt
     2041 atttatggct tacaacgttc cttctattat aaccgcctct agccccgaag aaccgtatgc
     2101 actactcatg ctgttaatgg cattggatgg tggcaatagt gggcgttttt ctaaagattt
     2161 gatccgcgat caatcgctta tcaccagcgt tttttctacg tatgacccct ttagtttaca
     2221 tgaagcattg atggaattta gcgcaatacc cacacaaaac cacacgctgg cagaagtcga
     2281 agcagcactg ctacagcaat tcgagcgatt aaaaacatcg cctattagcg atgctgaatt
     2341 aaaccgcgtt aaagttaatg cgattgcgca acatacctat gctaaagact ctatgtcgga
     2401 tcaagccatt gggttgggta gtttagaatc cgtcggactc tcttggaaac tggcggatac
     2461 ttttccggaa cagatccaaa cgattactgc ggaagatatc caaaaagtcg cacaaaagta
     2521 tctaacgcaa gaacggctaa caaccgctga actcatccct ttaaaatctg gaacttaatt
     2581 ttatgaaaaa tcgatattgg cgcattgtct tatttgtatt tgtcttaccc atcgtgctct
     2641 ttttttcatt ttcgaaaaaa gtagttaaaa cggaatctgc tgcaacttct gtagaaacaa
     2701 gtatgattca gcagtctacg actactccta cgcccatgcc catgcccacc cctataccac
     2761 atactgttgc catagaaaat tggtcaacca ccaatgggac gcgtgtttat tttgtcgcaa
     2821 cggaaggtat tcctattatt gatgtgcaag tcaattttga tgcgggatct gccagagacg
     2881 ctgaaaaacc tggactagct gcattatgtc ttcatttgtt agatcaaggg atccccaatg
     2941 caagcgcaga tttgattgcc gaaaaatttg aggattctgg cgcaaaattt agcgttgacg
     3001 tcgatcgcga tcgagccact atttcgctac gctccttaac cgatccaaaa cttttaaatc
     3061 ccgtgatcgc gctatatgtt gatttgatct caaacccaac gttccctgaa aacaatatag
     3121 aaactcaaag aaaccaaacg ctaatcgggc ttcaacgaaa tttacaacaa ccgaatgcag
     3181 tcgcaagtca agctttcttt aaggcacttt acaaaaacca tccttatgca gaacccgctt
     3241 gtggtaccat cgagggtgtt actttaatta caaaagcaga tcttgttcaa tttcatcaac
     3301 agtattttgt tgcaaaaaat gccaccatta cgattgtcgg cggtgtttct aaagaacaag
     3361 caaccgatat tgctaatcaa atttctgaaa aattgcctga aggaaacctg gcagcaccta
     3421 ttccaaacgt cgaaccttta atagaaccca ctcacgtcaa tatttctttt ccaaccgaac
     3481 aaacccatgt gttaacagga caaccttgtg cgacagaaaa cgaccccgat tattttccgc
     3541 tcttagtggg taataatatt ttaggcggtg gcatcttaac ttctcgcctg tttaatgaag
     3601 tccgcaatga ccgcgggtta gcttacaacg ttattagcgc tgtgcttcag ttacaaaaac
     3661 ctgctccctt tgtgatacga ttgcaaacaa aaaacagtca aacacaagaa gcgattacgg
     3721 tattacaaaa tacccttgtg aaatttataa acgaaggccc tacag
//
LOCUS       DAORVT010000097         3749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_19766_length_3749_cov_2.840823, whole genome shotgun sequence.
ACCESSION   DAORVT010000097 DAORVT010000000
VERSION     DAORVT010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19766_length_3749_cov_2.840823"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..923)
                     /gene="pgeF"
                     /locus_tag="PK389_02710"
     CDS             complement(153..923)
                     /gene="pgeF"
                     /locus_tag="PK389_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020396735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HQY22655.1"
                     /translation="MSSNYKSHDNWIVPTWPAPLNVKALTTVREGGSSLEPYDSFNFA
                     LHVGDDPRKVFANRAHLKLQAHLPQEPLWLTQTHSTRVVDVEDFADPNNPKNPIDADA
                     SVAFKPDQVCVVLTADCLPILLCNKAGTCVSAIHAGWRGLAAGIVEAAMSKLDCDPAS
                     LLAWLGPAIGPTAYEVQEDFLFTFKEYQSEIFFKPKENGRWFADIYQLARARLKKLGV
                     TAIYGGDFCTYQNSARFYSFRRSGITGRMATLIWYSRP"
     gene            complement(907..1515)
                     /locus_tag="PK389_02715"
     CDS             complement(907..1515)
                     /locus_tag="PK389_02715"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HQY22656.1"
                     /translation="MSTLVGRTAPDFTAAAVLGNGEIVSQYQLSKILKENKYALIFFY
                     PLDFTFVCPSELIALDCQIEQFKKLNVEVIGVSIDSQFTHNAWRNTTIEAGGIGPVNY
                     TLVADTNHSICQAYGVEHPEAGVAFRASFLIDPKGVVRSQMVNDLPIGRNIDELLRLV
                     DALQFHEAHGEVCPAGWKKGDKAMKPTPTGVASFLAEHVEQL"
     gene            1651..2034
                     /locus_tag="PK389_02720"
     CDS             1651..2034
                     /locus_tag="PK389_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22657.1"
                     /translation="MLLKQRRFLPLFCTRFLGAFNDSVFKNALIILFAFSTTKTFLNP
                     NTLVNLCALLFIVPFFLFSALAGEFADKFEKSALIRFIKLIVLLNLGLSIPYLFLLTG
                     LVNTGVAFFIFKQEPDFLKNKPLIK"
     gene            complement(2123..>3749)
                     /locus_tag="PK389_02725"
     CDS             complement(2123..>3749)
                     /locus_tag="PK389_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22658.1"
                     /translation="QLSNNKISFLKHYFLEKDTELKKELFSKIANNLTKIKEELSEAH
                     GQSLFPEEFISLALIRNVYLSKDQELINLFKENLPKPRIQLFEEYALSDEIKNEAEQT
                     VNALQIFLQDRKLPLVEFASIKTQRQLRAKKRQREEIARLAEQPQFLELKGKAEQELR
                     IKGALREANRHYIPKSPWKLLNKNIFVTASTIELFSTVHQLTSESGLVDIFDDALYGH
                     RTLQKLFKRFKCAALSPFDLMLGGDADVICCGPFEIDPRAKQQDFVDIVFDLDKLKGN
                     RCAFYKQLDLCYKKKKIRSIEIGNRTLYFSHTYPRQVRFGYVDLIIFEDGFPVATSRL
                     PNHTFISYNILQMHQILTLNFFRFLDNFKKLDGPIDLENLYMDGSPDEKRINSIYADI
                     NELNPTELRQFLIELGQNMSETAEFNIYGAYKIDFAAIKSITIRDKTLDFVQFVTELQ
                     SGEIEKLNATIQDLPTLFKSYRLLNYLLSKVQEAGAGNTAIQEKLLELKQSCSVPLLF
                     RKMDCQKVNIKTESSIKTLQRKPAPLPCSHAFL"
BASE COUNT         1131 a          765 c          655 g         1198 t
ORIGIN      
        1 aggctctaaa gcagggttat ctttgccgac tgcaatcgat tgtgcctctg aaagtgcaga
       61 ctgaaatgaa tgcgttaatt tatctaaact catagttagc cactccgtca cgttattcta
      121 taaaacataa gatgcggtct aatccacgca attcaagggc gagaatacca aatcagcgtt
      181 gccattcgac ctgtaatgcc cgacctacga aaggaataaa aacgcgcact gttctgatac
      241 gtacaaaaat cgccgccata aatggctgta acgcccaatt tcttcaaacg tgctcttgct
      301 aattgataga tatccgcaaa ccatcgtcca ttctcctttg gtttaaaaaa gatctctgat
      361 tgatattctt taaatgtaaa caaaaaatct tcttgcacct cataggcggt tggtccaatc
      421 gcaggtccca accaagccaa taagctagcg ggatcacaat cgagtttgct catcgctgct
      481 tctacaatgc ctgccgccaa tccccgccaa cctgcatgaa tagcactcac acacgttcct
      541 gctttgttac ataaaagaat gggcaaacaa tccgcggtta atacgacgca aacttgatca
      601 ggcttaaaag caacagaggc atcagcatct atcggatttt taggattatt gggatctgca
      661 aaatcctcaa catccacaac acgtgtactg tgtgtttggg ttaaccaaag aggttcttga
      721 ggtaaatggg cttgtaattt taggtgtgcg cggttagcaa agacttttcg gggatcgtct
      781 ccaacatgca gggcaaagtt aaaagaatcg taaggctcta gactgcttcc cccctcccgc
      841 accgtggtta atgctttaac atttaacggt gcgggccagg ttggcactat ccagttatca
      901 tgtgacttat aattgctcga catgttccgc caagaaggat gccacgccag tcggtgtggg
      961 tttcattgcc ttatcaccct tcttccaccc agcaggacag acttctccat gcgcttcgtg
     1021 gaattgtagc gcatcgacta aacgtaataa ttcatcaata ttccgcccaa tgggcaaatc
     1081 attcaccatt tgtgaacgta ccacaccctt gggatctatt aagaaagagg ctctaaaggc
     1141 aacacctgct tccggatgct caacaccata cgcctgacaa atactgtgat tcgtatctgc
     1201 aaccaaagtg taattaacgg ggccaatacc gccggcttcg atagtggtgt tacgccatgc
     1261 gttatgagta aactgagaat caatcgatac gccgattact tcaacgttta attttttaaa
     1321 ttgttcgatc tggcaatcaa gcgcaattaa ttccgaaggg catacaaaag taaagtccaa
     1381 tggataaaag aatattaaag cgtatttgtt ttcttttaaa attttagaaa gttggtattg
     1441 gctcacaatc tcgccattac ctaataccgc cgctgctgta aaatcgggag cagttctgcc
     1501 cactaaagtg ctaatcatat gagatactcc aagatgagac gaaataagct tcatcatacc
     1561 ataaaatgca gcgccatcat tatatctgta cattaataca gttttattgg catttgctaa
     1621 actgataaga taatcactca caatcctttt gtgttattaa aacagcggcg tttcttgccc
     1681 ttattttgca cacgattttt aggcgctttt aacgacagcg tctttaaaaa tgctttaatc
     1741 attttatttg cattttctac aacaaaaact tttttaaatc caaacacatt agtcaatcta
     1801 tgcgcgctat tgtttattgt gcctttcttc ttattttctg ccttagctgg agaattcgcc
     1861 gataaatttg agaaatcagc cttgattcgg tttattaaat tgattgtgtt gttaaacctc
     1921 ggattaagta ttccctattt atttttactc acaggtctgg tgaatacagg cgtagccttt
     1981 tttattttta agcaagaacc cgatttttta aaaaacaagc ctttgataaa ataagtacga
     2041 ataacaaaaa catcaaggtt gatatgcccg agtcagcctg gatgaaccta agctcttatc
     2101 aaaacgaaaa ttccttcctg tcctaaagaa acgcatgaga acacggtagg ggtgcaggtt
     2161 tcctttgcaa ggtttttata ctcgattcgg ttttaatatt aactttttga caatccattt
     2221 ttcgaaatag taaaggaaca gaacaagatt gttttagttc taaaagtttt tcctgaatgg
     2281 ctgtatttcc agccccagcc tcttgaactt tagaaagcaa ataattcaaa agccgatagc
     2341 ttttaaaaag agtaggaaga tcttgtatcg ttgcatttaa tttttctatc tcgcctgact
     2401 gtaattcagt tacaaattgt acaaaatcca gtgttttatc tctaatcgtt atacttttta
     2461 tagccgcaaa atcaatctta taagccccat agatattaaa ttcagcagtc tcactcatgt
     2521 tttgacctaa ctctatcaag aactgacgca attcagttgg atttaattca ttaatgtctg
     2581 catatatgct attgattctt ttctcatctg gggagccatc catatataag ttttctagat
     2641 ctataggacc atccagcttc ttgaagttat ctaaaaatct aaaaaaatta agggttaaga
     2701 tttggtgcat ctgcaggata ttgtaagaaa tgaaggtgtg atttggtagt cgtgaagttg
     2761 ctacaggaaa accatcttca aaaataatta aatcaacata accgaatcga acttgacgcg
     2821 gatacgtatg tgaaaaatat agtgtcctgt ttccaatttc gatgcttcta atcttctttt
     2881 ttttatagca caaatcaagt tgtttataaa aagcgcagcg attccccttt aatttatcca
     2941 aatcaaatac aatatctaca aaatcttgtt gttttgcacg tggatcaatc tcaaaagggc
     3001 cgcaacaaat cacatccgca tcaccgccta acattaaatc gaacggagat aaagcggcgc
     3061 atttaaatcg tttaaaaagt ttctgtaaag tcctgtgtcc ataaagtgca tcatcaaata
     3121 tatctaccaa tccactctcg ctggttaact ggtgtacagt agaaaaaagt tcaatagttg
     3181 aggcagttac aaaaatgttt ttatttaata acttccaagg gctttttggt atgtagtgcc
     3241 tattcgcttc tctcaacgca cccttgatac gaagttcttg ttctgctttt ccctttagtt
     3301 ccaaaaattg gggttgttca gcaagacgtg ctatttcttc tctttgtctt tttttcgctc
     3361 taagctgcct ttgtgttttt atactagcaa actcaaccaa aggtaatttg cgatcttgta
     3421 aaaatatttg aagtgcatta actgtttgct cagcttcatt tttaatttca tctgaaagcg
     3481 catattcttc gaaaagttga atacgtggtt ttggtaaatt ctctttaaaa agatttatta
     3541 attcttgatc ctttgataaa taaacatttc taatgagagc tagactaatg aactcttctg
     3601 gaaatagact ttgaccatgc gcttcagaga gttcttcttt tatcttagta agattattcg
     3661 caattttaga aaaaagttct ttctttaact cagtatcttt ttccagaaaa taatgtttca
     3721 gaaacgatat tttattattc gatagttga
//
LOCUS       DAORVT010000098         3698 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_20262_length_3698_cov_3.365358, whole genome shotgun sequence.
ACCESSION   DAORVT010000098 DAORVT010000000
VERSION     DAORVT010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20262_length_3698_cov_3.365358"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="PK389_02730"
     CDS             <1..767
                     /locus_tag="PK389_02730"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HQY22659.1"
                     /translation="ITLEYRGRKDKQKSIVLVGKGITFDTGGNSLKPAMSMIGMKYDM
                     CGAASVLGALRAAAELELPLNIIGVIAAAENMPGSKASRPEDVVTSMSGLTIEILNTD
                     AEGRLVLCDALTYSERFDPEVVIDIATLTNACVMALGSHTSGLFGNHNPLVNDLFNAG
                     LTSGDKCWQLPVWDEYQDALNSPFADIANIGNPPEAGAILAACFLSRFTKKYDWAHLD
                     IAGTAWRGGKDRAATGRPVSLLVQYLLDRCQKIKEG"
     gene            777..3614
                     /locus_tag="PK389_02735"
     CDS             777..3614
                     /locus_tag="PK389_02735"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013835934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HQY22660.1"
                     /translation="MINSMEDNKTYNPSVLESEWYPFWERSGYFNPRDTGVPYCIMLP
                     PPNVTGSLHMGHAFQQTLMDILIRYHRMKGRKTLWQVGTDHAGIATQMVVERQLLAKG
                     QTKYELGRDEFVNRIWQWKSESGGNISRQLRRIGASADWSHERFTLDEGMSKAVSDIF
                     IQLYNEGLIYRGKRLVNWDPVLKTAVSDLEVENQEEQGSLWTIRYPFAENLDTNSAIT
                     HNGKEFICVATTRPETLVGDVAVAVNPTDPRFTHLIGKHLQLPLTDRVIPIIADDMVD
                     PAFGTGCVKITPAHDFNDNAMAQRHNLPLINIFTIDATLNENAPTQYQGLDRFVAREK
                     IIEDLRAKNLLEKIEPHLLKIPRGDRSNAILEPLLTDQWFVSIKSLAEPAIEAVKNGQ
                     IRFVPENWTKTYFDWMNNIQDWCISRQLWWGHRIPAWYDDAGKIYVGESEIAVRAHYK
                     LPLDLPLKQDEDVLDTWFSSALWPFSTLGWPENTARLQTFYPTQVLVTGFDIIFFWVA
                     RMIMFGLKFTGKIPFHEVYIHGLIQDQNGEKMSKTKGNVLDPIDLIDGIDLESLVKKR
                     VTGLMQPQLASQIEAYTRIQFPTGISAYGTDALRFTYAALATHGRHIRFDLRKIESNR
                     HFCNKLWNAARFVLMNTEGKMETPSNTQVLSAVNHLSPVDQWILSRWQQTKAQIEKHL
                     AEYRFDLAAQAIYEFTWNEYCDWYLELTKPILNPRLENQNMKNGTRHTLISVLEELLR
                     VIHPFMPYITEALWQRVAPVANKTGDTIMLQPYPEYIEGLINTNAENDIEWIKTIVLA
                     IRNIRGEMNLPPSKPVPVLFYNGTTEDQRRLDSDFYQLILKSMAKIESIRWLLPNEQR
                     EESVTALVGSLEIIIPLAGLIDKSAEIERLKKEIIKSQADFEKIKVKLLNPHFSEKAP
                     PEVVAAEQKRAIELKALIDNLTNRLLALTPL"
BASE COUNT         1129 a          815 c          761 g          993 t
ORIGIN      
        1 taatcaccct agaatatcgc ggacgcaaag ataaacaaaa atccattgtg ctagttggca
       61 aagggattac ttttgataca ggcggtaatt cgcttaaacc ggctatgagc atgattggca
      121 tgaaatacga tatgtgtggc gcagccagcg tgctcggtgc actgcgtgct gccgcagaat
      181 tagaattgcc acttaacatt atcggcgtga ttgccgccgc agaaaatatg cccggcagta
      241 aggcgtcccg cccagaagac gttgtaacca gtatgtcggg tcttacgatt gagattttaa
      301 ataccgacgc cgagggtcga ctggttttat gcgatgcgct tacctattct gagcgttttg
      361 atccagaagt cgttattgat attgcaacct taaccaatgc ttgtgtgatg gcactaggtt
      421 cgcataccag cggccttttc ggtaaccaca acccactcgt taatgatctc ttcaacgcag
      481 gtttaacgag tggtgataaa tgttggcaat tgcccgtatg ggatgaatac caagatgctc
      541 taaacagccc atttgccgat atcgccaata tcggaaatcc ccccgaagca ggcgcaattc
      601 tcgccgcatg tttcttatcg cgatttacca aaaaatacga ttgggcacat ttagatatcg
      661 ccggaacagc ttggcgtggc ggaaaagatc gcgctgcaac tgggcgaccc gtctctctac
      721 tagttcaata cctgctcgat cgctgtcaaa aaataaaaga aggctaaagg tctgatatga
      781 taaattcgat ggaagataat aaaacctata atcccagcgt cttagagagc gaatggtatc
      841 cgttctggga gcgttctggc tactttaatc caagggacac aggtgtaccc tactgcatta
      901 tgctacctcc gcccaatgta acgggcagcc tacacatggg acacgcattt caacaaacat
      961 taatggatat tttaatccgt tatcatcgca tgaaaggccg aaaaacctta tggcaagtgg
     1021 gcactgatca cgcgggtatt gcaacgcaaa tggtagtcga aagacaattg ctcgccaaag
     1081 gccaaacaaa atacgaatta ggccgagacg aatttgttaa tcgcatctgg caatggaaat
     1141 ccgaatctgg cggtaacatt agccgtcaat tacggcgtat cggtgcctct gccgattggt
     1201 ctcatgagcg ctttacttta gacgaaggca tgtctaaagc tgttagcgat atttttattc
     1261 aattatacaa tgaagggtta atttatcgcg gcaaacgatt ggtcaattgg gatcccgttt
     1321 taaaaacagc cgtttctgat ttagaagtcg aaaaccaaga agaacaagga tcgctttgga
     1381 caattcgtta tccctttgct gaaaacttag ataccaattc tgctataact cacaatggca
     1441 aagagtttat ctgtgtcgcc acaacgcgac ccgaaacctt agtaggcgat gtggctgtcg
     1501 cggttaatcc aacggatccc cgttttacac atttaattgg caaacactta caactaccct
     1561 taaccgatcg cgttattccg attattgcag atgatatggt cgatcctgct tttgggacag
     1621 gttgcgtaaa aattacgccc gcgcacgatt ttaatgacaa tgcgatggca cagcgtcaca
     1681 acttaccact cattaatatt tttacgatag atgcaacact caacgaaaac gcacccacgc
     1741 aatatcaggg attagatcgc tttgttgcac gcgaaaaaat catcgaagat ctgcgtgcta
     1801 aaaatttgct cgaaaaaatt gaaccgcatc tcttaaaaat tcccagaggc gatcggagta
     1861 acgctatttt agagccttta ttaaccgatc aatggtttgt cagtataaaa tcactggcag
     1921 aaccagcaat tgaagcagtc aaaaatggcc aaattcgttt tgtacccgaa aattggacca
     1981 aaacctattt tgactggatg aataacatcc aagattggtg cattagccga caactgtggt
     2041 gggggcatcg aatccccgct tggtatgacg atgccggcaa aatttatgtt ggagaatctg
     2101 aaatagctgt tcgtgcacat tacaaacttc cacttgattt gccccttaaa caagacgaag
     2161 atgttttaga tacctggttc tcttcggctt tgtggccctt ttcaacctta ggctggcctg
     2221 aaaacacagc aaggctccaa accttttatc caacccaagt gcttgtcacc ggttttgata
     2281 ttatcttctt ctgggttgct cgcatgatta tgtttggtct taaatttacg ggcaaaattc
     2341 ccttccatga agtttatatt catggactca ttcaagatca aaatggcgag aagatgtcta
     2401 aaactaaagg caatgtactc gaccccattg atttaatcga tggcattgat ctcgaaagcc
     2461 ttgttaaaaa acgcgtaacg ggcttaatgc aaccccaact tgcctctcaa atcgaagctt
     2521 atacacgcat acaatttcca acggggatct ccgcttatgg tacagatgcg ttacgattta
     2581 cctatgccgc tttggcaaca cacggacgac atattcgctt tgatttaaga aaaatcgaaa
     2641 gcaaccgaca tttctgtaat aaactctgga atgctgcccg ctttgtgtta atgaataccg
     2701 aaggcaaaat ggaaacacct tcaaacacgc aagttttgtc tgctgttaat catttatcgc
     2761 ctgttgatca atggatactt tctcgttggc aacagactaa agcacagata gaaaaacatc
     2821 tagcagaata tcgcttcgat ttagcagcac aggcaattta tgaatttacc tggaatgaat
     2881 attgcgattg gtatttggaa ttaacaaaac cgattttaaa cccgcgttta gaaaatcaaa
     2941 atatgaagaa tggtacgcgc catacgctta tatccgtact agaagaatta ctacgcgtca
     3001 ttcacccttt tatgccctat attacagaag ccctttggca acgggttgca cctgttgcaa
     3061 acaaaacagg cgatacaatt atgctccagc cctatccaga atatatagaa ggactcatta
     3121 acacgaacgc tgaaaatgat attgaatgga ttaaaaccat tgtccttgcg atacgaaata
     3181 tacgcggaga aatgaacctt ccgccatcta aacctgtacc tgttttgttt tacaatggaa
     3241 ctacagaaga ccaaagacga ctcgattctg atttttatca attaattcta aaatcgatgg
     3301 ctaaaattga aagtattcga tggttattac caaacgaaca acgtgaagag tccgtcacag
     3361 cgttagtcgg atctttggaa ataattattc ccttagccgg cttaatcgat aaaagcgcag
     3421 aaattgagcg tttgaaaaaa gaaataatca aatctcaagc tgattttgaa aaaataaagg
     3481 ttaagctttt aaatccacat ttttctgaaa aagcacctcc tgaagttgtc gcagcagaac
     3541 agaaacgcgc aatagaactc aaggcactta ttgataactt aaccaatcgg ttgttagcct
     3601 taaccccact ttgaaaaaaa aggggaggcg atgagcaagc tctgccgagc aattttactg
     3661 cttcacttta cacggtgcct caatttgtac gcgcaact
//
LOCUS       DAORVT010000099         3669 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_20547_length_3669_cov_3.522690, whole genome shotgun sequence.
ACCESSION   DAORVT010000099 DAORVT010000000
VERSION     DAORVT010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20547_length_3669_cov_3.522690"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /gene="fabZ"
                     /locus_tag="PK389_02740"
     CDS             complement(<1..285)
                     /gene="fabZ"
                     /locus_tag="PK389_02740"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013746660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HQY22661.1"
                     /translation="MVLDIQQILERLPHRYPFLLVDRVLEFEQDKTLLAIKNVSFNEP
                     FFSGHFPVKAVMPGVLILEALAQAAAILASHTAEWDPNGSLFYLGSIENAR"
     gene            complement(286..1359)
                     /gene="lpxD"
                     /locus_tag="PK389_02745"
     CDS             complement(286..1359)
                     /gene="lpxD"
                     /locus_tag="PK389_02745"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HQY22662.1"
                     /translation="MKDSQFYTLGALADLIGARLRGDPSCLISGIGSLRGAKAGEISF
                     LSNAEYRKDSPSLYRRDLALTKASAVILSSAEAPLCPVHLLVSENPRLSLAKAAKIFE
                     KKESLIPGIHKSALIGEGCEIPDSVSIGANVVLGNRVILGENVVVAPGCIIGHDCVLG
                     NNTTLMARVTLYDNVQMGKHCLIYSGAVIGGDGFGFAHQEGSWIRLPHLGSVVMGDQV
                     EIGANTTIDRGFLEDTLLGDGVVIDNLVQVGHNVSIGAGTAIAGCVAIAGSAVIGKFC
                     LVGGGSRIAGHVEIVDHVHLSGASMVTHSLRVPGVYGGFPAKPVDLWRKNIARFQNLD
                     DMARRLRALENRVLNNARLVEDE"
     gene            complement(1367..1936)
                     /locus_tag="PK389_02750"
     CDS             complement(1367..1936)
                     /locus_tag="PK389_02750"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HQY22663.1"
                     /translation="MKYLIRSILLSVCLGLGAMAVTADELPTRIGVLDWQLLLSKSPQ
                     AEDAGKRLEKEFQAPKDKFVNKQKEFQTKREKMQRDADVMSLAERGKKEKELAKMEQD
                     LRRMDEELRSDYATRHREEMDLFIKEVKDVVDKVAEEYKYDLVLSQEAALYVAERIDI
                     TGKVLERLEKTAKSSPAKKAEATKSDKKS"
     gene            complement(1974..>3669)
                     /gene="bamA"
                     /locus_tag="PK389_02755"
     CDS             complement(1974..>3669)
                     /gene="bamA"
                     /locus_tag="PK389_02755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HQY22664.1"
                     /translation="SDDKYNKEKLNADLETLRSYYMDRGYINFQIDSSQVSLSSDKKS
                     IFITIHITEGEKYNFRHVSLAGQFIVPKEELDSLLLPLEKGTAFSRKTLLEVKQALEN
                     RLGDEGFTFAEARTNHEIIEDKKSVDIVFEMHPGKRMSVRRILFKGNSTTKDEVLRRE
                     LPQMEGTWISNALVKEGKEKILRRGFGSEIEIETPAVPGTTDELDVVYKLEEARLGQL
                     SGGLGYSPTEKLMFNFAISQENFFGTGNAVDFNFDKSKATTSYALGYMDPYFTVDGIG
                     MGASGYFNQSNLSKTTNISNFTTDTLGGELRWIFPVDKYEFIKLSAGYDDTRLKIDSL
                     LAPREIQNFTNRYGTQFREYAVGVTWIYETLNQRIFPTRGLSQSAGVRTVVPGANQQY
                     YQLSYSALAYKPLTESELWVVGLKGNFSYGSGYGKTSRLPFYRNSYAGGMGSVRGFEE
                     SSLGPKDSSGRSFGGNALILGTASLIFPNPIKPDAKSVRTALFLDAGQVYDTSYTKSI
                     VNGTSRNPRGLRYAAGVSLTWNTPLGAPLSFSFAKPINAKRGDETRYFAFSMATQF"
BASE COUNT         1080 a          900 c          627 g         1062 t
ORIGIN      
        1 acgtgcattc tcaatagatc ccaaatagaa taaagagccg tttggatccc attccgcagt
       61 atgactcgcc aaaatagcag ccgcttgcgc caatgcttct aaaattaaga ctcccggcat
      121 taccgcttta actggaaaat gacctgaaaa aaaaggctcg ttaaaggaga cattttttat
      181 agccaacaat gttttatctt gttcaaattc taaaacacga tccaccaaca aaaatggata
      241 tcgatgtggc aaccgctcta aaatctgctg gatatctagt accatttatt catcctccac
      301 aagacgtgca ttgttcaata ccctattttc caaagcacgc aaccgtcgtg ccatatcatc
      361 taagttctga aagcgtgcga tattttttcg ccataaatca accggtttcg ctggaaatcc
      421 accatatacg cctggtaccc ttaacgaatg tgtcaccata gatgcacctg agagatgtac
      481 atgatcgaca atttcaacat gtccagcaat acgtgaaccg ccaccaacta aacaaaattt
      541 tccaatcact gcacttcccg cgatagcaac acatcccgca atagctgttc ctgcaccaat
      601 agaaacgtta tgaccgactt gtactaaatt gtcgataaca accccatcgc ctaacaatgt
      661 atcttctaaa aatccacgat caattgtcgt atttgcccca atttcaacct gatcgcccat
      721 caccacgcta cccaagtgcg gcaaccgtat ccaagaacct tcttgatgtg caaatccaaa
      781 accatcacca ccaataaccg cgccactgta aatcaagcag tgtttaccca tttgaacatt
      841 atcatagagt gtgacacgtg ccattaacgt tgtattatta ccaagtacgc agtcatgtcc
      901 aataatacat cctggtgcga caacgacatt ttcacctaaa ataacacggt tacctaaaac
      961 aacattcgcg ccaatagaaa cagaatccgg aatttcgcaa ccttcgccta tcagcgcgct
     1021 tttatgaata ccggggatta ggctttcttt tttttcaaaa attttagcgg cttttgctaa
     1081 acttaatcgc ggattctcgg aaacaagcag atgtaccgga caaagtggag cctcggcact
     1141 gctcagaata acagcagatg cttttgttaa agctaagtct ctacggtata gtgaaggtga
     1201 atctttgcgg tattccgcat tgcttaaaaa agagatctct cctgctttcg cacctcttaa
     1261 agacccaata cccgaaataa gacagctcgg atcgcctcga aggcgcgcgc cgattaaatc
     1321 agcgagcgcc cccagtgtgt aaaattggct atccttcatt cgtgaattag gatttcttat
     1381 ccgacttagt cgcctcagcc tttttagcgg gtgatgactt agccgttttt tctaatcgtt
     1441 ccaaaacttt tccggtgata tcaattcttt ctgcaacata caatgccgct tcttgtgata
     1501 aaaccaaatc atacttatat tcttccgcaa ctttatcaac cacgtctttc acttctttaa
     1561 taaagagatc catttcttct cgatgtcggg ttgcatagtc cgatcttaac tcttcgtcca
     1621 tacgacggag atcttgttcc attttagcca attctttttc ttttttacca cgttctgcta
     1681 gcgacatcac gtctgcatca cgctgcattt tttcgcgttt agtttgaaac tctttctgct
     1741 tatttacaaa cttatctttg ggcgcttgaa attctttttc aagacgcttg ccagcatctt
     1801 ctgcttgagg cgattttgat aacagcaact gccaatctaa aacaccaatt ctggttggca
     1861 attcgtccgc agtcacggcc attgcaccaa ggcctaaaca aactgataac aaaatggatc
     1921 ttattaaata tttcaagcta actctcctcg caggcaaact gctaaataac caactaaaat
     1981 tgagtggcca tcgaaaacgc gaagtaacgc gtctcatcac cccgctttgc attgattggc
     2041 tttgcaaaac taaatgacag cggcgctcct aaaggcgtat tccaagtaag ggatacaccc
     2101 gctgcatacc tcaacccacg cgggttgcga gatgtgccat tcacgatact tttcgtgtag
     2161 ctcgtatcat atacctgacc agcatctaaa aacaatgctg ttcgaaccga ttttgcatct
     2221 ggtttaatcg gatttggaaa aataagagat gcagtcccta aaatgagcgc attaccccca
     2281 aacgaacgac cactcgaatc cttgggtcct aatgaacttt cttcaaaacc gcgaacagaa
     2341 cccatacctc cagcatacga atttctataa aaaggcagtc gagacgtttt tccataacca
     2401 gacccataag aaaagttacc tttcagacca acaacccaca attcagactc tgttagcggc
     2461 ttatacgcca atgctgaata ggacaactgg taatactgct gatttgcacc tggaacgacc
     2521 gtcctaacgc ctgcagattg tgataaaccc cgggttggga atatgcgttg atttaaagtt
     2581 tcgtaaatcc aagttacacc aacggcatac tcacgaaact gtgttccata gcggttagtg
     2641 aaattttgaa tttctctcgg cgcaagtagt gagtcaattt ttaatcgtgt atcatcataa
     2701 cccgcagaaa gttttataaa ttcatacttg tctacaggaa atatccaacg caattctcca
     2761 ccaagtgtgt ctgttgtaaa attagaaata ttcgtggttt tactcagatt agactggtta
     2821 aaataaccac tggcgcccat tccaatccca tcgaccgtaa agtaaggatc catgtaaccc
     2881 aatgcgtagc ttgtcgttgc cttacttttg tcaaagttaa aatcaactgc attaccggtt
     2941 ccaaagaaat tttcttgaga aatggcaaag ttaaacatta atttttcggt aggcgaataa
     3001 ccgagaccac cactcaactg tcctaaacgc gcttcttcaa gcttataaac aacatccaat
     3061 tcgtcggttg tgccaggcac cgctggtgtt tcaatttcta tttcagaacc aaacccacgt
     3121 cgcaaaatct tttctttgcc ttctttaacc agggcattcg aaatccacgt gccttccatt
     3181 tgaggaagct cacgtcgcaa cacttcatct tttgtggttg aattgccttt gaataagatc
     3241 cggcgcacac tcatgcgttt accagggtgc atctcaaaaa cgatatcaac agattttttg
     3301 tcttctataa tttcatggtt agtgcgtgct tccgcaaaag taaaaccttc gtctcctaat
     3361 cgattttcaa gtgcttgttt cacttctaat aatgtctttc tagagaatgc cgttcccttt
     3421 tctaagggca aaagcaaaga gtctaactct tctttaggta caataaattg tccggccaaa
     3481 ctcacatgcc gaaagttata tttctcgcct tcggttatat gtatggtgat aaaaatactc
     3541 tttttatccg aagacaagga aacctgagaa gaatcgattt gaaaattaat atacccgcga
     3601 tccatataat aagatcgtaa tgtttctaaa tctgcgttta atttttcttt attatattta
     3661 tcatcgcta
//
LOCUS       DAORVT010000100         3660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_20633_length_3660_cov_2.896810, whole genome shotgun sequence.
ACCESSION   DAORVT010000100 DAORVT010000000
VERSION     DAORVT010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20633_length_3660_cov_2.896810"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="PK389_02760"
     CDS             complement(<1..588)
                     /locus_tag="PK389_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HQY22665.1"
                     /translation="MSWVDDRFPLTKLWNEHLAHYYAPKNFNFWYYFGSLALFVLVNQ
                     IVTGIWLTMNYTPTAEGAFDSIEYIMREVPWGWLLRYLHSTGASFFFIVVYLHMFRGL
                     LYGSYKKPRELVWIFGMLIFLCLMAEAFFGYLLPWGNMSFWGAQVITSLFGAIPYVGE
                     ALVEWIRGDYNITGVTLNRFFALHVIAVPLIFLGLV"
     gene            complement(618..1205)
                     /gene="petA"
                     /locus_tag="PK389_02765"
     CDS             complement(618..1205)
                     /gene="petA"
                     /locus_tag="PK389_02765"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HQY22666.1"
                     /translation="MSVDEMDTGRRRFLTATASVIGGVGVACAAFPFLASWAPSVKAR
                     ALGSPVEVDISKIEPGQKITVAWRGQPVFIVHRTHEELNGLSLVTQELRDPLSKESVQ
                     PAYADNIYRSIKPDILVLIGICTHLGCVPLYKPTVGSIEPGWEGGFFCPCHGSKYDMA
                     GRVYKGVPAPLNLVVPPYKFLSDTIIVVGTDGVAA"
     gene            complement(1301..1759)
                     /locus_tag="PK389_02770"
     CDS             complement(1301..1759)
                     /locus_tag="PK389_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22667.1"
                     /translation="MINERIKVMEHIASTHESRILTALEHLKNTFPLTEESVGHLSDD
                     QLLNIEMLTSRFAKLQDYLGTKVFDIFFEVEQENVDGFTMIDKLNKLEKLGIINDAHQ
                     WRDMRGARNIVEHEYPDNPLLIASNLNKINDMIPFLIAIKRRIFEKVRKI"
     gene            complement(1756..2085)
                     /locus_tag="PK389_02775"
     CDS             complement(1756..2085)
                     /locus_tag="PK389_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HQY22668.1"
                     /translation="MDAHFSKRVRLKEFEIKAILQCFARYFLLDDHLWVFGSRADIQK
                     KGGDIDLYIETKMNSREVYKAKMDFLHAVCDKIGDQKIDIVVHLIDDNIKLPIYMIAK
                     TEGVQLV"
     gene            complement(2138..2211)
                     /locus_tag="PK389_02780"
     tRNA            complement(2138..2211)
                     /locus_tag="PK389_02780"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2177..2179),aa:Gln,seq:ctg)
     gene            complement(2287..2790)
                     /gene="rpsI"
                     /locus_tag="PK389_02785"
     CDS             complement(2287..2790)
                     /gene="rpsI"
                     /locus_tag="PK389_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012070100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HQY22669.1"
                     /translation="MIVTAKKTLASGKKRQCYATGRRKTSTARVFLRPGTGNITVNTQ
                     GLDVYFGRKTARMVVRQPLELIEVTDKFDVVVTVKGGGIMGQAGAIRHGISRALIKYD
                     EEINPPKVVEAIEGEEAVEAAADSSSTSFRRILRKAGFVTRDSRKVERKKIGLHGARK
                     RPQYSKR"
     gene            complement(2803..3231)
                     /gene="rplM"
                     /locus_tag="PK389_02790"
     CDS             complement(2803..3231)
                     /gene="rplM"
                     /locus_tag="PK389_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010181416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HQY22670.1"
                     /translation="MKTFSAKSEEIQHAWFLVDAKGKVLGRLASEIARRLKGKHKPEY
                     TTHVDTGDCIVVINAGDIRVTGRKAKDKLYHHHSGYPGGLKEESFESLVARRPERVIE
                     LAVKGMLPKNPLGRAMLRKLKIYAGAAHPHSAQQPEILDI"
BASE COUNT         1137 a          848 c          662 g         1013 t
ORIGIN      
        1 aaccaaccct agaaaaatta gcggtacggc aatcacatgc agggcaaaaa aacggtttaa
       61 ggtaacacct gtaatattgt aatcacctct gatccactca actaacgctt ctcccacata
      121 aggaatagcg ccaaataaag aggtaatgac ttgcgcaccc caaaaagaca tattgcccca
      181 cggcaataag tatccaaaaa acgcctctgc cattaagcat aaaaatatca gcataccgaa
      241 gatccaaacg agctctcgcg gttttttata agagccatac aaaagccctc taaacatatg
      301 caaataaaca acaataaaga aaaaagaagc gcctgtagaa tgtaagtaac gcagcaacca
      361 gccccaaggg acttcgcgca taatatattc tatagaatca aaagcacctt ccgcagtggg
      421 ggtatagttc atcgtgagcc aaatacctgt tactatttga ttcactaaaa caaaaagagc
      481 gagcgatcca aaataatacc aaaaattaaa attcttaggc gcgtagtaat gtgctaagtg
      541 ctcattccac aatttcgtta aaggaaaacg atcgtctacc cagctcatta aataccgcga
      601 ggctgatctc aattttatca cgcggctaca ccatcggttc cgactacaat aatcgtatcg
      661 ctcaaaaact tataaggtgg aaccaccaaa tttaagggcg ctggcacgcc tttatacaca
      721 cgtcccgcca tatcgtattt agaaccatga cacggacaaa aaaagccccc ttcccaaccg
      781 ggttctatcg atccgacggt tggcttgtaa agcggcacac agcctaaatg cgtacaaatg
      841 ccaattaaca ctaaaatatc gggtttaatc gaacggtaaa tattatcggc gtaggcgggt
      901 tgaacggatt ctttagataa aggatctctc aattcttgag tcaccaagct taagccattt
      961 aattcttcgt gcgtacgatg cacaataaaa accggctgac ctcgccaagc aacagtgatt
     1021 ttttggccgg gttcaatttt gctgatatcc acctcaacag gcgaacctaa agcgcgcgct
     1081 tttacgctgg gtgcccaaga agcgagaaaa ggaaaagcag cacaggcaac accgacgcca
     1141 ccaataacag atgccgtagc tgttaaaaac cgtcgacgtc cggtatccat ttcgtcgaca
     1201 ctcattctca tcaactcctt gtatcgggtg cctaagatag gagatgttat cagaatttca
     1261 gcaattctgc atagcgcaaa taaacatttt gcatttggcc ttaaattttt cgcacttttt
     1321 caaaaatacg tcttttaatt gcaattaaaa aaggtatcat gtcattaatt ttatttaaat
     1381 tactggctat aagcaatggg ttatcaggat actcatgctc aacaatgttc cgtgcaccgc
     1441 gcatatcacg ccattgatga gcatcattaa tgatccctag tttttctaac ttatttagtt
     1501 tatcaatcat cgtaaatccg tctacatttt cttgctcaac ctcaaaaaaa atatcaaaca
     1561 cctttgtgcc taaataatct tgaagcttag caaatctact tgtcaacatc tctatattca
     1621 gcaattgatc atcagaaaga tgccctacag attcttctgt taatgggaat gtatttttta
     1681 aatgctctag agcggttaaa attctactct catgcgtaga tgctatatgt tccattactt
     1741 taattctttc atttatcata caagttgcac gccttcagtt ttagcaatca tgtaaatagg
     1801 tagtttaata ttatcatcaa tgaggtgtac aacaatatct attttttgat cgccaatttt
     1861 gtcacacact gcgtgtaaaa aatccatttt agccttatag acttctctcg agttcatctt
     1921 tgtttctatg tagagatcga tatctccacc ttttttttgt atgtccgcac gtgaaccaaa
     1981 cacccaaaga tgatcatcca ataaaaaata tctcgcaaaa cattgaagga tcgctttgat
     2041 ctcaaattct tttaatcgaa cccgttttga aaaatgcgca tccatacaga tatttacctg
     2101 ataactgtgt aaccatttga tctcacaagc gaaattctgg ctgggggact aggattcgaa
     2161 cctaggttaa cagagtcaga gtctgtagtc ctaccactag acgatccccc aatcggggta
     2221 gcaattccct tctcccgttt agcgggagaa ggtggcgcat agcgccagat gagggggtga
     2281 aaaagcttat cgcttagagt attgaggtct cttacgtgca ccatgcaagc caattttctt
     2341 acgttcgact tttctggaat ctcgtgttac aaaacctgct tttcgcaata ttcttctaaa
     2401 gctcgtgcta gaactgtcag cagcggcttc aacggcttct tcgccttcta ttgcttcaac
     2461 tacttttggt ggattaatct cttcatcgta tttaatcaaa gcacggctaa tcccatgacg
     2521 aatcgcgccc gcttgtccca taataccacc gcctttaacg gtaaccacca catcaaattt
     2581 atcggtgact tcgatcaact ctaaaggttg tcgaacaacc atacgagccg ttttgcgacc
     2641 aaaatacaca tctaaacctt gtgtattaac cgttatgtta cctgtgccag gtcttaagaa
     2701 aacccgtgcg gtagaagtct tacgacgacc ggttgcataa cattggcgtt ttttcccaga
     2761 ggctaaagtc ttttttgctg ttacaatcat atttctacct agttagatat ctaaaatttc
     2821 aggttgttga gcagaatgtg gatgcgcagc gccggcataa attttcagtt tacgcaacat
     2881 cgcacgccct aatggatttt tgggaagcat accctttact gcgagctcta ttacacgctc
     2941 tggacgacgc gcaaccaagg attcgaaaga ttcttccttc agaccgcctg gatagccact
     3001 gtggtgatga taaagcttat ctttggcttt tctgccagtt actcgaatat cacctgcatt
     3061 tatcaccacg atacagtctc ccgtatcgac atgcgttgta tattctggct tatgtttacc
     3121 ctttaaacgt cgagcaattt cgctcgccaa gcgtcctaaa accttgccct tagcatcgac
     3181 taaaaaccaa gcatgctgga tttcttcgga ctttgcactg aatgttttca ttaacctagg
     3241 tccccagtta gagaatcaaa ttacacaaac aaatatgtag aagcggatct ctgtgtccgc
     3301 ccaacaagaa ggggcatcat acaaaactat gaataaaaat acaaggcgca aaaagacaaa
     3361 actcgaaaat cggtacagaa agcgccttaa cagagatgtg tcttgctgca aaaacgctca
     3421 aaagcgacca aaaacaacaa agaatgcatc tacaattgac ttctaaaaca gctctagggt
     3481 agcatgaacg cgatcttagg aaatttaccc gcttaatcgc ggttaggtta tcaagacgaa
     3541 aaagttgtag tactctaaaa agcgtgtaaa aaaccttgct gaatgcaagc gttatgccta
     3601 cactagttta tgtttgattt ttttaaggag catgacatgg ccgcgaaaaa acgcaagccc
//
LOCUS       DAORVT010000101         3648 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_20757_length_3648_cov_3.074868, whole genome shotgun sequence.
ACCESSION   DAORVT010000101 DAORVT010000000
VERSION     DAORVT010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20757_length_3648_cov_3.074868"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3447)
                     /gene="dnaE"
                     /locus_tag="PK389_02795"
     CDS             complement(<1..3447)
                     /gene="dnaE"
                     /locus_tag="PK389_02795"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HQY22671.1"
                     /translation="MTDPQFVHLHLHSEYSLVDGLVRIDECCDAIVSQKSSAVAITDQ
                     CNLFAMVKFYQAAESAGIKPIIGTDVWLMNEADPSQPFRLILLCQNMVGYRNLTEIVS
                     RSYVEGQFADKPQIKKAWLSEKNTGLILIASARESDIAKALLGTDSESESAEKLVKEW
                     FAIFNDRFYLEIIRTNRSDEGRYNQALVALAEKTGCPLVATNDVRFINREDFEAHEAR
                     VCIHEGTTLSDPKRSKNFSEEQYLKSPFEMQALFSDLPEAIENSVEIAKRCNFELKLG
                     KIYLPNYPVPVDTTIQQYFIDLAHQRLKNKFANHPIPDLYKDRLNTELTVLNSMGYAG
                     YFLIVADFIQWARDNKIPVGPGRGSGAGSLVAFALGITGLDPLQYDLLFERFLNPERV
                     SMPDFDIDFCMEGRDRVIDYVANTYGRESVSQIITYGTMAAKAVIRDVGRALGHPYGF
                     VDKIAKLIPMELGITLEKALNQEEVLKQRYSDENEVKTLIDLAKKLEGVTRNAGKHAG
                     GVVIAPSKLIDFTTLYCDAKEGHLLTQFDKDDVEAIGLVKFDFLGLRTLTIIDWAVEA
                     INKINLKNNKEHIDIEKIPLDDVEVFDLLKACNTTAVFQLESRGMKDLVKRLQPDNFE
                     EIIALVALFRPGPLQSGMVDDFINRKHGREIPEYLDPKLTHILKPTYGVILYQEQVMQ
                     IAQVLAGYTLGAADLLRRAMGKKKPEEMAKQRAIFVKGSVERGVSEHSATHIFDLMEK
                     FAGYGFNKSHSAAYALITYQTAWLKTHYKEAFMAAVLSSDMANTDKIVVFVHECSENK
                     ISIVPPDVNKSLFKFSVDEQNRILYGLGAIKGVGESAIEGIIEARAAKPFQDIFDFCS
                     RIDGRKVSRRVLEALVRAGAFDCFGVDFTQRAEWMETLSDAVHAAEQISKNRSRGQTD
                     LFGDSFSAEDIKASAKPKNIEPWSESVRLQGEKESLGLYLTGHPLAEYEEELSHIRTS
                     TIRDLKVERNQSCVITGLIVTVRTLMTRKGDRMAFVTLDDRTGRQEIAIFSDQYRENK
                     ELLVKDNLVIVTGEVSIDTYSGGYKMRCKEIMSLEKSRLKSAKFLKVVVSSLELGENF
                     AKKLMDTIKPYQVQEGCPVQIVYERNESLVFLRLGEDWRVNPKETLVDTLERLPGIAS
                     VEV"
     gene            complement(3449..>3648)
                     /locus_tag="PK389_02800"
     CDS             complement(3449..>3648)
                     /locus_tag="PK389_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004916745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22672.1"
                     /translation="EMVILDKAFPEYGFAKHKGYPTAMHRMILTMRGPCKIHRLSFGP
                     VKEALKRSLANSLVQTEMTAD"
BASE COUNT         1094 a          852 c          589 g         1113 t
ORIGIN      
        1 aacctcaacg cttgcaatgc cgggtaaccg ctctagggta tcaaccaacg tttctttggg
       61 atttactcgc caatcttctc ctaaacgcaa aaacaccaaa gattcattgc gctcataaac
      121 gatctgtaca ggacaccctt cttggacttg atagggtttg atggtgtcca tcagcttctt
      181 tgcaaaattt tcacccaatt ccaaagacga gacaaccact tttaaaaact ttgccgactt
      241 taatctggat ttttctaaac tcatgatttc cttgcaacgc attttgtaac cacctgagta
      301 cgtatcgata ctcacttctc cagtgactat caccaaattg tctttgacta aaagttcctt
      361 gttctctcga tattgatccg aaaaaatagc aatttcttgt ctacccgttc tatcatctag
      421 ggtaacaaaa gccatcctgt cgcctttgcg cgtcatcaac gtacgtactg taacaattaa
      481 tccggtaatc acacaacttt gattccgttc cacttttaaa tcacgaatcg tgctggtgcg
      541 aatatgcgat aattcttctt catattctgc caaaggatga cctgttaaat aaagtcctaa
      601 agactctttt tcaccttgta aacgaaccga ttcagaccaa ggttcgatat ttttaggttt
      661 cgcagacgcc ttgatatctt cggccgaaaa agaatcgcca aataaatccg tctgaccacg
      721 cgatcgattt ttagagattt gctccgcagc atgaacggca tcgcttaatg tttccatcca
      781 ttcggcacgc tgtgtaaaat ctacaccgaa acaatcaaaa gcacccgcac gcacaagcgc
      841 ttctaaaaca cgacgactca ctttacgacc gtctatgcga gaacaaaaat caaatatgtc
      901 ttgaaaaggt ttcgcagcgc gtgcctcaat aataccttct atagcagatt ctccgactcc
      961 tttaattgca cctaatccat ataaaattcg attctgttcg tctacagaaa atttaaacag
     1021 acttttattc acgtcaggcg gcacaatact aattttgttt tcagaacatt catgcacaaa
     1081 aacaactatc ttatccgtat ttgccatatc agaagacaat accgctgcca taaaggcttc
     1141 tttgtaatgc gtttttaacc aggcggtttg atacgtaatc aacgcgtaag ccgcagagtg
     1201 tgatttatta aaaccatagc ctgcaaattt ttccattaaa tcaaaaatat gggttgctga
     1261 gtgctcagac acacctcttt cgactgaacc cttcacaaat atagcacgct gttttgccat
     1321 ttcttcgggt tttttcttac ccatcgcacg ccgcaacaaa tcggctgcgc ctaaagtata
     1381 tcccgccaac acttgggcaa tttgcatcac ctgttcttga tataaaataa ccccataagt
     1441 cggctttaaa atatgcgtta atttcggatc caaatattcg ggaatttcgc gaccatgttt
     1501 gcgattaata aagtcatcga ccataccaga ctgcagcggt cctggtctaa ataaagcaac
     1561 caaggcaata atttcttcaa aattatccgg ctgtaatcgt ttaaccaaat ccttcatacc
     1621 acgagattct aactgaaaca cagcggttgt attgcacgct tttaataaat caaacacttc
     1681 tacgtcatct aaaggaattt tctcaatatc aatgtgttct ttattgtttt tgagattgat
     1741 tttattaatc gcttccacag cccaatcaat aatcgtgagc gttcttaaac ccaaaaagtc
     1801 gaactttacc aagccaatcg cttctacgtc atctttatca aattgcgtta ataaatgtcc
     1861 ctcctttgca tcgcaataga gcgtcgtaaa atcgatgagt ttcgatggcg caatcacaac
     1921 accgccggca tgcttccccg catttcgagt aacgccttct agtttttttg ccaaatcaat
     1981 cagtgtttta acttcgtttt cgtctgaata gcgttgcttt aaaacctctt cttgatttaa
     2041 ggctttttct aaggtaatac ccaactccat ggggattaat tttgcgattt tatcgacaaa
     2101 tccataagga tgtcctaatg ctcgccccac atctcgaata acagcttttg cagccattgt
     2161 gccataagta ataatttgtg aaacggattc tcgaccatag gtatttgcca cataatcaat
     2221 aacgcgatcc cgaccttcca tgcaaaagtc aatatcaaag tcgggcattg aaacgcgttc
     2281 tgggttcaaa aatcgttcaa aaagtaaatc atattgcaag ggatccaatc ccgtaatacc
     2341 cagagcaaaa gcaacaaggg aaccagcacc cgacccacga cccggaccta cgggaatttt
     2401 attatcacgt gcccattgaa taaagtctgc gacaattaaa aaataacccg cataccccat
     2461 cgaatttaaa accgttaatt ctgtgtttaa tctgtcttta tacaaatcgg gaatcggatg
     2521 attagcaaat ttgtttttta acctttgatg tgccaaatct ataaaatact gttgaatcgt
     2581 tgtatcaacc ggcactggat aattgggtaa ataaattttt ccaagcttta attcaaaatt
     2641 acaacgtttg gcaatttcaa cgctattttc aatcgcttct ggcaaatctg aaaagagtgc
     2701 ttgcatctcg aaaggtgact tcagatattg ttcttcacta aaatttttag aacgttttgg
     2761 atcacttaat gtcgtacctt catgaataca cacccgtgct tcgtgtgctt caaaatcttc
     2821 cctgtttata aatcgaacat cattagtcgc aacgagagga catcctgttt tttctgctag
     2881 cgcaaccaat gcttgattat accttccttc atcactgcga ttagttcgta tgatctcgag
     2941 ataaaatcga tcattaaaaa tggcgaacca ctctttaact aatttttcag cagattctga
     3001 ttcagaatca gtccctaaca acgctttagc gatatcactt tctcttgcag aagcaatcag
     3061 aatgagtccc gtgttttttt cactaagcca cgcctttttt atttgcggtt tgtccgcaaa
     3121 ttgaccttcg acataagaac gagacacaat ttcagttaaa ttgcgataac ctaccatgtt
     3181 ttgacaaagc aaaatcaatc gaaaaggttg actgggatcc gcttcattca ttaaccaaac
     3241 atctgtacca ataatcggtt ttatgccagc cgattctgct gcttgataaa acttgaccat
     3301 tgcaaacaaa ttacattgat ctgtaatagc aacggcacta cttttttgac taacaatcgc
     3361 gtcgcagcat tcatcaatac gaacaagtcc gtccaccaac gaatattcac tgtgtaaatg
     3421 taaatgtaca aattgaggat ctgtcatttt agtctgctgt catttctgtc tgtactaaac
     3481 tatttgcaag agaccgtttc aaagcttctt taacaggtcc aaaacttaag cgatgaattt
     3541 tacaagggcc acgcattgtt aaaatcatcc gatgcattgc cgttggataa cctttatgtt
     3601 ttgcaaaacc atattccgga aatgctttat ctaaaataac catttcgc
//
LOCUS       DAORVT010000102         3647 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_20762_length_3647_cov_4.665924, whole genome shotgun sequence.
ACCESSION   DAORVT010000102 DAORVT010000000
VERSION     DAORVT010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20762_length_3647_cov_4.665924"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            291..800
                     /locus_tag="PK389_02805"
     CDS             291..800
                     /locus_tag="PK389_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22673.1"
                     /translation="MVNHCLRKKYHRLTPWLLLLVFFGPWLTAQLIYVKRDQLAFKTI
                     ETGTLVSPPIQAQSLPFYDSAWLGKWQIVYMAPQNDHDTDVIPMLNQLHKALGKDKHR
                     VAYQIIATKGALKQHPSAKEVFNLVLLAPGEIALIDPRGWLMMHYPANPDPIGMLKDI
                     RRLLRYSHG"
     gene            793..1860
                     /locus_tag="PK389_02810"
     CDS             793..1860
                     /locus_tag="PK389_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017052950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HQY22674.1"
                     /translation="MAKRLAYGATLLAFVVVVLGAYTRLTNAGLGCPDWPGCYGQLVP
                     QIDATNPLINAAKAWTEMIHRYLAGSLGIIIFVLAFFIVKNKSRFNKQSQILAITVSA
                     LVIFQALLGMWTVTLKLFPVVVMAHLMGGLATLSLLWWLTLTLQSHHTALYKNNFSFF
                     SSPSSPCANMRERQSKSYFCTKPRALRIWGIIGLIMLLIQIFLGGWTSANYAALVCLD
                     FPFCDNFNQNLGLNFQFANIVKAFNFTAVGVDGSQGTPLGYAARIMIHMLHRIGALLT
                     TLIIGWLVLLTWMRSQNKSIRIVSIMMISLLIIQFFLGITNVLAKLPLPIAVAHNAVA
                     ALLLLSVVTLNYFIFTKIYEK"
     gene            1850..2737
                     /gene="cyoE"
                     /locus_tag="PK389_02815"
     CDS             1850..2737
                     /gene="cyoE"
                     /locus_tag="PK389_02815"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011331793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HQY22675.1"
                     /translation="MKNNSHHSSLIADYFTLCKPKVILVMLITTWVGMHLATNTYVPW
                     NTFIFATLGIALAGGSAAVINHLVDRHIDAKMTRTQSRPMANKRISIQSACIFSAILG
                     VSGLLILAVFINPLTTYLTFVTVLGYAVLYTLFLKRQTPQNIVIGGAAGAMPPLLGWT
                     AVTGHTSAFAWLLVLIIFTWTPPHFWALAIHRKNDYENANIPMLPNTHGIPFTKLYIV
                     LYAILLLVVTTLPYAAHMAGGIYFGAASFLGTAFLVQTLILYRTEANNTALKTFSFSI
                     LYLLLLFSALLIDHYIQLP"
     gene            2751..3392
                     /locus_tag="PK389_02820"
     CDS             2751..3392
                     /locus_tag="PK389_02820"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HQY22676.1"
                     /translation="MYIKRVFLTVGLTLAAVFIVWYGVKPILEKNAPPIIMPEITTAT
                     ILKPKNVVTNFSLTDTTNQPFTEKSLLGQWTLMFFGYAQCPEICPKTLIKITELWKQV
                     PQDLHFVFVSLDPESDTVQILRDFLGRFNSEFRGLTGEAAVIQKLAKACGIYSWQDPK
                     ANDPSKPKMIDHSATLLLINPQGQLHAVFSPPYHPEIMAKDLKQILSTSIQNR"
     gene            complement(3410..>3647)
                     /locus_tag="PK389_02825"
     CDS             complement(3410..>3647)
                     /locus_tag="PK389_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22677.1"
                     /translation="RYRIIFEKMQRELKEKLDRCKKPLAPFDFETVQIRWNPLPGFQR
                     HLTREREEAVAVAAAGAEAVHPSATNTQSSPDTL"
BASE COUNT         1017 a          803 c          704 g         1123 t
ORIGIN      
        1 gtgcctgaaa tatttcacat caataccgat cccaaccatc ccttattgaa acaagcagct
       61 caatttattc gttcagaata caatatgctt ttggaacaag atcgtagtca taaaaagtca
      121 tcataattcg ctgaattaaa attcgtctac atctaatatg ttcttgcaat gaaagaaaaa
      181 agatgactcc aaacgctagc ataaatctaa cacttgggcc aaacgcacta atcggttaca
      241 atgcccattg aaacattcaa tgaaatttta aatgtacgtt attttgacat atggttaatc
      301 actgcttaag gaagaaatac catcggctga cgccttggct gctactcttg gtcttttttg
      361 gaccctggct aacagcgcag ctgatctacg tcaaacgtga tcaattggct tttaaaacaa
      421 ttgaaacagg cacactcgtc tctccgccta tacaagcgca atcgctaccc ttttatgatt
      481 ctgcctggct aggaaaatgg caaatcgtct atatggcacc ccaaaatgat catgatacag
      541 atgtcatacc catgctaaac cagctacaca aagctttagg taaagacaag catcgtgttg
      601 cctatcaaat tatcgcgaca aaaggcgcat taaagcaaca cccttctgca aaagaagtat
      661 ttaatctcgt gctattagcc ccgggcgaaa ttgcgctgat tgatccccgt ggttggttaa
      721 tgatgcacta tccagccaac cctgatccga ttggcatgct aaaagatatt cgccgattat
      781 tacgatattc acatggctaa acgattagca tacggcgcaa cgctactcgc ttttgttgtg
      841 gttgtattag gtgcgtacac gcgtttaaca aatgcaggtc ttggttgtcc cgattggcca
      901 ggctgttatg gacagctggt tccacagata gatgccacaa atcctctgat taacgcagct
      961 aaagcttgga cagaaatgat ccatcgttat cttgcgggat cattaggtat cattattttt
     1021 gtgctggctt tttttatcgt taaaaacaaa tcgaggttca acaaacaatc tcaaatactg
     1081 gcaattacgg tgagcgcttt ggttattttt caagcgctat tgggcatgtg gacagtcacg
     1141 ctcaagttat ttccagtggt tgtgatggca catcttatgg gtggactcgc aacgctgtcg
     1201 ctgctctggt ggcttacttt aacattacaa tctcaccata cggctctgta caaaaataat
     1261 ttttcttttt tttcctcgcc aagctcgccc tgcgcaaaca tgcgtgagcg acaaagtaaa
     1321 agttattttt gcacaaagcc tcgcgcccta cgcatatggg gaataattgg gcttatcatg
     1381 cttctaatac aaatatttct cggcggatgg acaagtgcaa attatgctgc tttagtgtgt
     1441 ttagattttc cattttgtga taattttaat caaaatctgg gtttaaattt ccagtttgcc
     1501 aatatagtta aagcttttaa ctttacagct gtaggtgtcg atggtagcca gggtacacca
     1561 ctcggctatg ctgcacgcat aatgattcat atgctgcacc gcataggcgc acttttaaca
     1621 accctgatta ttggatggct tgtattactg acatggatgc gatcacaaaa taagtctatt
     1681 cgaatagtta gtattatgat gataagtctg ttaatcatac aatttttttt aggcatcaca
     1741 aatgttttgg caaagttacc cttaccgata gcggttgcac ataatgcagt tgccgcgtta
     1801 ttattgctta gcgtcgtcac tttgaattat tttatattta cgaaaatata tgaaaaataa
     1861 tagccaccat tcaagcctta ttgcagatta ttttacgtta tgcaaaccca aagttatttt
     1921 ggtgatgttg atcacaactt gggttggcat gcacctcgca accaacacat acgtgccgtg
     1981 gaataccttt atttttgcaa cactcgggat tgctttagcc ggcggttctg ctgctgtgat
     2041 taatcatctg gtcgatcgcc atatcgatgc aaaaatgacg cgtactcaaa gccgccctat
     2101 ggccaacaaa cgcatatcga ttcaaagcgc ctgtattttt tcggctattt taggcgtgag
     2161 tggattactg atattagctg tttttatcaa tcccttaacc acctacctga cctttgtaac
     2221 cgttttaggc tatgccgttc tatatacact ttttctgaaa cgccaaacac cacaaaatat
     2281 tgttatcggt ggtgctgcag gtgcaatgcc ccctttgtta ggctggacag ctgtcacagg
     2341 ccataccagt gcattcgctt ggttattggt acttattatt tttacctgga cacctccaca
     2401 tttttgggct ttggcgattc atcgaaagaa tgactatgaa aacgccaata tccccatgtt
     2461 accaaatacg cacggcatcc catttacaaa attatatatt gtgttatatg ccattttatt
     2521 gcttgtggtg acgacgctgc cctatgctgc gcatatggcc ggagggattt atttcggtgc
     2581 agccagcttt cttggaactg cttttttagt tcaaacgcta attttatatc gcacagaagc
     2641 taacaacacg gctctaaaaa catttagttt ctccattctg tatttgttgt tattatttag
     2701 tgcattgctg attgatcatt acattcaact gccataggag gcttttgtag atgtacataa
     2761 aacgcgtttt tttaactgtt ggtctcacgc tcgccgctgt ttttatcgtt tggtatggcg
     2821 ttaaacccat tctcgaaaaa aatgcgccac ccatcatcat gcctgaaatt accacggcaa
     2881 cgattttaaa acccaaaaat gttgtgacaa atttttctct caccgacacg acaaaccaac
     2941 cctttacaga aaaatcactt ttaggacaat ggacgctgat gttctttgga tatgcgcaat
     3001 gtccggaaat ttgccccaaa accttaataa aaattaccga gctttggaaa caagttccac
     3061 aagatttaca ttttgtattt gtaagcttag atcctgaaag tgatacggtt cagatcctgc
     3121 gagatttctt agggcgcttt aattctgagt ttagaggctt aaccggagaa gcagctgtta
     3181 tacaaaaact ggctaaagca tgtggaatct acagttggca agatcccaaa gcaaatgatc
     3241 ccagcaagcc taaaatgatt gatcacagcg caacactctt gctcatcaac ccccaaggtc
     3301 agctacacgc tgtcttttca ccaccttatc atccagagat catggcaaaa gatttgaagc
     3361 aaatattgtc tacctctata caaaatcgat aggcaattta gcgcacctcc tatagcgtat
     3421 ctggactcga ctgcgtattg gtggcgcttg gatgcacggc ttctgcgcct gctgctgcca
     3481 cagcgacagc ctcttctctt tctcttgtca aatggcgttg gaagcctggt aaagggttcc
     3541 agcgaatctg tacggtttca aaatcaaaag gtgccaatgg ttttttacaa cggtctaatt
     3601 tttctttgag ttccctttgc attttctcaa aaatgatgcg atagcgt
//
LOCUS       DAORVT010000103         3624 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_20991_length_3624_cov_2.967218, whole genome shotgun sequence.
ACCESSION   DAORVT010000103 DAORVT010000000
VERSION     DAORVT010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20991_length_3624_cov_2.967218"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /locus_tag="PK389_02830"
     CDS             complement(<1..1205)
                     /locus_tag="PK389_02830"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HQY22678.1"
                     /translation="MLEVELPRPVIKTKPYSRDLEQQGLAAGLHPIIARVIASRSISS
                     DVPLEHALFPKLKNLFSPFNMADMEKAASRVARAIIEGECIGFETDHDCDGQTSHAVL
                     YHNLVHHFKHPKEKIRSYIGNRLTEGYGLSESVMMRILEDTPRAQLIITADNGSSDEA
                     RIKLLFEAGIDVIVTDHHEIPQEGLPVSAYACLNPTRSDCRYGDPYIAGCMVAWLLMT
                     ATRQKLIALGHLSANTPTLLDSLDFVAVGTIADCVSIARSINNRAVVSYGIKIIESGT
                     RPCWRAIKTTLSGPLCSEDLGFKIGPLLNSDGRLATAFGSVSFLLSETDEEAMAWLFA
                     LQDSNQERKSIQQDIVLRGLKEAKCQLDQGRSSLCIYLEESHPGVHGIAASRLKDIFG
                     RPTAFIAPKV"
     gene            1254..1622
                     /locus_tag="PK389_02835"
     CDS             1254..1622
                     /locus_tag="PK389_02835"
                     /inference="COORDINATES: protein motif:HMM:NF016326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HQY22679.1"
                     /translation="MQFTLDNGDAAFQIKAYEKGVIIVNEQTYNYPILVMPAHLIAPW
                     GPDSFEELAPTHFEMILPYKPKLVILGTGKQHRFLPASLYAALINNNIGVEIMDTKAA
                     CRTYTVLMAEGREVIAGLFC"
     gene            complement(1611..3251)
                     /locus_tag="PK389_02840"
     CDS             complement(1611..3251)
                     /locus_tag="PK389_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011477077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQY22680.1"
                     /translation="MISISHVSMHYAAKLLFDEVNLNLLPGNRYSLVGANGTGKSTFL
                     KLLAGDDTPSLGEIIVAKQASIGFLKQDQFRYENNTVLEVVLQGKPVLWQALQEKEKI
                     LSNDTLDEKAGHRLAVLEDIIFHHDGYTAETFIQTLLIGLGVEQGYHLKPLNALSGGF
                     KLRALLAQALFGQPDILLLDEPTNHLDIMSIAWLENYLKTQIKGVLLFISHDYGFLNN
                     LSTHVLDVDFGEIREYVGNYDHFMEEKKLKVEQKLNELQYLENKIAHMRTFVDKFRAG
                     TRSKQAQSKEKMIDKLELPDIKKSSRVSPYFNFKQKRPSGKIVLKTEKISKNFGEKIV
                     LKNVSFSINRGEKVAIIGHNGIGKSTLLKILLAQHVADTGTYEWGYESHIAYFAQDHH
                     ETLKQNMGVLDWLRQERESDNTQDAIRRALGQMLFGQDDVHKRIPTLSGGEAARLLFA
                     NMILLQANIIILDEPTNHLDLESREALALALSKFEGTVIFVSHDRHFVSSIATRVLAL
                     TEKGITDFAGNYEEYLSKFGEDYLNKVWLMEQEKQLTK"
     gene            3366..3593
                     /locus_tag="PK389_02845"
     CDS             3366..3593
                     /locus_tag="PK389_02845"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HQY22681.1"
                     /translation="MSGLIMSTAAGYPPSFFGLFQFPQFIAKNAAIAHFFFNVHEVTG
                     YIIIALVIIHTAAALKHHFTDRDNILKRMLP"
BASE COUNT         1060 a          801 c          651 g         1112 t
ORIGIN      
        1 actttaggtg caataaaggc cgtgggacga ccaaaaatat ctttcaatcg gctagccgca
       61 ataccatgca cgccaggatg gctctcttct agataaatac ataaactgct gcgcccttga
      121 tcaagctgac attttgcttc ttttaatcct cgtaaaacaa tatcttgttg aatcgatttt
      181 cgttcctgat tagagtcctg caaggcaaat aaccacgcca tcgcttcttc atctgtctcg
      241 gacaacaaaa agctcacgga accaaaggcc gttgccaaac gaccatcgct atttaataac
      301 ggcccaattt taaatcccaa atcttctgaa cataaaggcc ctgataaagt cgtttttatt
      361 gcacgccaac agggacgtgt tcccgattca atgattttta tgccataaga cacaaccgct
      421 ctattattaa tacttctcgc aatactcaca caatccgcaa tggtgcctac tgcaacgaaa
      481 tctaatgaat ccaacaacgt tggcgtatta gccgataaat ggcctaaggc aattaatttt
      541 tggcgagttg ccgtcattaa caaccatgca accatacacc cagcaatata cggatcgcca
      601 taacgacaat ccgatcgcgt aggatttaaa caagcataag cactaaccgg taatccttct
      661 tgtggtattt cgtgatgatc ggtcacaatc acatcgatac ctgcctcaaa cagtaattta
      721 atacgtgctt catctgagga gccgttatct gcggtaataa tcagttgtgc tctcggcgta
      781 tcttctaaaa ttcgcatcat tacagattca gataaaccat atccttctgt taatcgattg
      841 ccgatataag aacgtatttt ttccttggga tgtttaaaat gatgcactaa gttgtgatac
      901 aaaacagcat gactggtttg accatcgcaa tcatgatcgg tttcaaaccc aatgcattct
      961 ccttctataa tcgctcttgc cacgcgactg gctgcttttt ccatatctgc catattgaat
     1021 ggcgaaaata agtttttcag ttttggaaat aaagcgtgtt ctaagggcac atcgcttgaa
     1081 atcgatctcg aagcaatcac acgtgcaata ataggatgca gaccagcagc caaaccttgt
     1141 tgttccaaat cccgactata gggctttgtt tttatgacgg gtcttggtaa ttcaacttct
     1201 aacacaaccc tctccttcaa ggtattttaa tcaatactct gtataatgaa tatatgcaat
     1261 ttaccctaga caatggcgat gccgcatttc aaattaaggc ttatgaaaaa ggcgtcatca
     1321 tagttaatga gcagacttac aattacccga tcctggtaat gccagcacat ttaatcgccc
     1381 catggggtcc agattctttt gaagaactcg cgccaacgca tttcgaaatg attttgccct
     1441 acaaacctaa gcttgtcata ctgggtacag gcaagcaaca tcgatttttg ccagcatctc
     1501 tctacgctgc actgataaac aataacatcg gtgttgaaat aatggatact aaagcggctt
     1561 gccgcaccta taccgtactg atggcagaag gacgcgaagt tatagcggga ttattttgtt
     1621 aattgctttt cctgttccat taaccaaact ttgtttaaat aatcttcgcc aaatttcgag
     1681 agatattctt cataattgcc agcaaaatct gtgatgcctt tttcggttaa agccagtacc
     1741 cgtgtcgcaa tactagacac aaaatgccga tcatggctaa cgaatataac cgttccctca
     1801 aatttagata aagctaaggc caaggcttcg cgcgattcta aatctaagtg attggtaggt
     1861 tcatccaaaa taataatatt ggcttgtaat aaaatcatat tggcaaataa taaacgtgcg
     1921 gcctctcccc cacttaaagt tgggatacgt ttatgcacat catcttgccc gaacaacatc
     1981 tgtccaagcg ctctacgaat ggcgtcttgc gtgttatcac tttctctttc ttgacgtagc
     2041 caatctagca ctcccatatt ttgcttaagt gtttcgtgat gatcttgagc aaaatacgca
     2101 atatgacttt cgtatcccca ttcgtaggtg cctgtatctg caacatgctg cgccaaaaga
     2161 attttcaaca aggtcgattt cccaatacca ttgtgtccaa taatagcgac tttttcaccg
     2221 cggttaatcg aaaaagaaac attttttaaa acaatttttt caccaaagtt tttagaaatt
     2281 ttttctgttt ttaatacaat ttttccggac ggtctttttt gtttaaagtt aaaataggga
     2341 ctcacgcgag aagatttttt aatgtcgggc aattctaact tgtctatcat tttttcttta
     2401 gactgagctt gcttcgaacg cgtgcccgcc ctaaatttat cgacaaaagt acgcatatgt
     2461 gcaattttat tttctaaata ttgaagttcg ttcagttttt gttcaacttt gagctttttc
     2521 tcttccataa aatgatcata attgcccaca tattctcgaa tttcgccaaa atcgacatct
     2581 aaaacatgcg tggaaaggtt gtttaaaaaa ccataatcat gtgatataaa caataaaacg
     2641 cctttaattt gtgtttttaa atagttttct aaccaggcaa tactcataat atctaagtga
     2701 ttcgtcggtt catcgagtaa taaaatatcc ggctgaccaa aaagagcttg tgccaatagc
     2761 gcacgcagtt taaaaccgcc cgacagtgca ttgagtggtt ttaagtgata tccctgctcc
     2821 acccctaagc cgattaacaa agtttgaata aacgtttctg cggtatatcc gtcgtggtga
     2881 aaaataatat cttccagtac ggcaagtctg tgccccgctt tttcatcgag ggtatcgttg
     2941 ctcaatattt tttctttctc ttgcagtgct tgccaaagca cgggcttgcc ctgcagaacc
     3001 acttcaagca cggtattatt ctcgtatcga aattgatctt gctttaaaaa gccaatgctg
     3061 gcctgcttgg cgacaataat ttcccctaaa ctgggtgtat catcaccggc taataatttt
     3121 aaaaatgttg atttgccggt gccattagcc ccaaccaggc tatagcgatt gccgggaagc
     3181 aggtttaaat taacttcatc aaaaagtaac tttgctgcat agtgcatgga gacatggcta
     3241 atagaaatca taaaaattct tctggaaaat gaaagtctgg cagtatacca taatccatag
     3301 ggacagaccg ctgtgtctgg ccttttataa aggggtaaag ttagccgata agctatgggg
     3361 cgcctatctc tggactgatt atgtctaccg ccgccggata cccacccagt ttttttggat
     3421 tatttcaatt tccgcaattt atcgcaaaaa acgcagctat tgcacacttc ttttttaatg
     3481 tgcatgaagt gacggggtat attattattg ctttggttat tattcatact gctgcggctt
     3541 taaaacacca ttttaccgat cgtgataata ttttaaaacg catgttgcct taagcaagga
     3601 gttttatttg gatataaaac ccca
//
LOCUS       DAORVT010000104         3623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_21004_length_3623_cov_2.538677, whole genome shotgun sequence.
ACCESSION   DAORVT010000104 DAORVT010000000
VERSION     DAORVT010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21004_length_3623_cov_2.538677"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PK389_02850"
     CDS             complement(<1..405)
                     /locus_tag="PK389_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HQY22682.1"
                     /translation="MEFSYLAASTALIVAFWALAYLAAPLWAWTLTFALSLGVMQAHA
                     FFTGPLQASLWGILAFWIVIFHLSFFRQRFLTTFIFRVFKNKMPRMSSTEREATEAGD
                     IWWESELFRGRPNWDRFLAAPQQSLTQEELNFL"
     gene            586..888
                     /locus_tag="PK389_02855"
     CDS             586..888
                     /locus_tag="PK389_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22683.1"
                     /translation="MVRTHEYPVIGVWYWDIEHADQFEIVAEDKLDRSIEIQYFSGEI
                     EEVDIDTWFEMRVVSIAAPKDWSGPYEIEKDNFSDLEELEEEGKHPKGWSDPFDEI"
     gene            1050..1610
                     /gene="lexA"
                     /locus_tag="PK389_02860"
     CDS             1050..1610
                     /gene="lexA"
                     /locus_tag="PK389_02860"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HQY22684.1"
                     /translation="MKLTKRQQEILDLIQSHINAAGSPPTRAEIAKTMGFRSPNAAED
                     HLRALARKGVIELVPGTSRGIRLLKTTGGIPIIKIDRIVSHQPILSDNHIEGMCKLER
                     NFFKPSIDFLLQISHKGYPEHHIATGDYLAIHQTQEIPAGQLAVTRLNHEISIQRYTT
                     DNFPQNLIIEGLVIGVIRTLNNSSLC"
     gene            1610..2692
                     /gene="mnmA"
                     /locus_tag="PK389_02865"
     CDS             1610..2692
                     /gene="mnmA"
                     /locus_tag="PK389_02865"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008899351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HQY22685.1"
                     /translation="MTHPIAKRVIVGLSGGVDSSIAALLLQKQGFQVEGLFMKNWQED
                     DNNHCPATQDLMDAQKVCENLCIPLHTINFSNQYWDNVFEHALAEYRLGRTPNPDILC
                     NREIKFKTFLDYAIELGADYIATGHYAAISENNNIFQLHKAKDLNKDQSYFLYTLNQT
                     ALSKTLFPIAHLEKPFVRKLAAEAGFPNCDKKDSTGLCFIGERKFKNFLNTYLPTQKG
                     NMETPDGKIIATHDGLMFYTLGQRQGLGIGGRHNGDNAPWYVVGKDLARNVLIVAEGD
                     NHPLLYAHTLTANQLHWVRGSAPQTHFTCTAKIRYRQTEEPATVEMLNAETCIVTFEN
                     PQRAITPGQSVVFYKETVCLGGGIIQ"
     gene            2689..3333
                     /gene="hflD"
                     /locus_tag="PK389_02870"
     CDS             2689..3333
                     /gene="hflD"
                     /locus_tag="PK389_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013819619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HQY22686.1"
                     /translation="MITSSLHLRTLALAGVFQAATLVKQLAKTGRVDQPAFLASIQSI
                     FKIDVDSATDVYELPENLALGLRELIQLFTNNQLPKDPEIARYVFSLLHLEKKLSKNT
                     KMLLQLRTGIKRATLQAAHFSPTHENVMANLASLYTDTLSTFSFRIHVTGDPLYLNQT
                     YHVNKVRALLLAGVRSAVLWQQLGGRRWQLLLSRTSILHTAHEWLNELITEETV"
     gene            3369..3611
                     /locus_tag="PK389_02875"
     CDS             3369..3611
                     /locus_tag="PK389_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22687.1"
                     /translation="MSPMWSDLWKDLTEKFQSIETAKNDLLHSNDLPHPAEENAPPKN
                     KTLTLQLDREKADNFSQQILQLSKEPKGGPSSKPND"
BASE COUNT         1151 a          830 c          686 g          956 t
ORIGIN      
        1 caaaaaattt aattcttctt gtgtgagcga ctgttgaggc gctgctaaga agcgatccca
       61 attgggcctc cctctaaata attcggattc ccaccaaata tcacccgctt cagtcgcttc
      121 cctttccgta ctactcatcc tgggcatctt gtttttaaaa acacgaaaaa taaaagtggt
      181 gagaaacctt tgtctaaaaa acgataaatg aaatatcaca atccaaaaag ccagtatgcc
      241 ccagaggctc gcttgcaagg gtcccgtgaa aaacgcatgc gcttgcatta ccccaaggga
      301 aagcgcaaat gtcagcgtcc aggcccacaa gggcgctgca agataggcca gcgcccaaaa
      361 agcaacaatg agcgctgtgc tcgcggctaa atatgaaaat tccattgatt cgctccctct
      421 gcctgttgcg tacactttga aggtacacct aaattttaga acaaactcaa acccttcagt
      481 gaaaaaaaaa tcatttttaa aaaataaata tcccaattct taatttagtt gataaactat
      541 caattacagg attacaaatt ttaaacagaa ggggagcaga aaatcatggt acgtacacat
      601 gaatacccag tgataggcgt gtggtattgg gacatagagc atgctgatca atttgaaatc
      661 gtcgctgaag acaaattgga caggtccatt gaaatccaat acttttcggg agaaattgaa
      721 gaggttgata tagatacctg gtttgaaatg cgtgtcgtct ctatcgctgc gcctaaagat
      781 tggtcaggcc cttatgaaat tgagaaggac aacttctctg atttggaaga attggaagaa
      841 gaaggtaaac acccaaaagg atggtctgat ccatttgatg agatctgacc tcactcgctg
      901 ctacacggca ccctctctcg taaaacaagg gggtgtaatg agtgacctat tgatttttaa
      961 acagtctgga caacagaaca gcattgtatt aagatagtca ttggccttag acttcaattc
     1021 aacccacagg aagttttatg actaaaccaa ttaagctaac gaaaagacag caagagatcc
     1081 tcgatttaat ccaatcgcac atcaatgcgg caggttctcc acccactcgc gcagaaattg
     1141 caaaaaccat gggatttcgt tctcctaatg ccgctgaaga tcacttaaga gcgctagcac
     1201 gcaaaggcgt cattgaactt gttcccggca cctcacgtgg catacgcctt ttaaaaacga
     1261 ctggaggtat tcccattata aaaatcgatc gcattgtctc tcatcagccc atcttgtctg
     1321 ataaccatat tgagggaatg tgcaaactcg aacgcaactt ttttaaaccc agcattgatt
     1381 ttttattgca gatttcgcac aaggggtatc cagaacacca tatcgcaaca ggtgactacc
     1441 tcgccattca ccaaacgcaa gagatccctg ctggtcaact cgctgtaaca cgattaaatc
     1501 acgaaatcag catccaacgt tacacgacag ataactttcc ccaaaatctt atcatcgaag
     1561 gattagtgat aggcgttatt cgaaccttaa ataactcttc tctatgctaa tgacacaccc
     1621 gatagcaaaa cgcgttattg ttgggctttc gggtggtgtt gattcctcta tcgccgccct
     1681 acttttacag aaacaaggct ttcaagttga gggccttttt atgaaaaact ggcaggaaga
     1741 cgataataat cattgtcctg ccacacaaga tttgatggat gcccaaaaag tttgtgaaaa
     1801 cttatgcatt ccacttcaca caattaattt ttcaaatcaa tattgggaca atgtctttga
     1861 gcatgcctta gcggaatatc gattaggacg aacgccgaat cccgatattt tatgtaaccg
     1921 tgaaattaaa tttaaaactt ttctagatta tgcaatcgaa cttggcgccg attatattgc
     1981 gaccggacat tatgccgcta tttctgaaaa taataatatt tttcagctgc ataaagccaa
     2041 agatctgaac aaagatcaaa gttattttct gtataccctc aaccaaacag cactttcaaa
     2101 aactttattc ccaatcgcgc atttggagaa accttttgtc cgaaaattgg cagcagaagc
     2161 tggctttccg aattgcgata aaaaagacag tacgggactt tgttttattg gtgaacgcaa
     2221 atttaaaaat ttcctcaaca cctacttacc cacgcaaaaa ggtaacatgg aaacacctga
     2281 tggaaaaata atagccacac atgacggctt gatgttttac actttgggtc aacgacaagg
     2341 gttaggtata ggtgggcgtc acaatggcga taatgctccc tggtacgtcg tcggcaaaga
     2401 tctggcacgc aatgtattga ttgtggcaga aggcgataat cacccacttc tctatgctca
     2461 cactttaaca gccaatcaat tacattgggt aagaggtagt gctcctcaaa cgcactttac
     2521 atgcaccgca aaaattcgat atcgacaaac agaagagcct gcaaccgtcg aaatgctaaa
     2581 cgcggaaact tgcatcgtca cctttgaaaa tccacaacgt gcaattacac caggtcaatc
     2641 cgtggttttt tacaaagaga ctgtttgttt gggtggaggt attattcaat gataacttca
     2701 tcattacacc tacgaacctt agctttagcc ggcgtttttc aagcagcgac tttagtaaaa
     2761 caactcgcaa aaacaggacg tgtcgatcaa cctgcttttt tggcgagcat ccaaagcatt
     2821 tttaaaatcg atgtagacag cgcgacggac gtctacgaat tgcctgaaaa tttagccttg
     2881 ggattacgag aacttatcca acttttcacc aataatcagc tccccaaaga tcctgaaata
     2941 gcccgttacg ttttttcttt gttacatctc gaaaaaaaac tctcaaagaa tactaaaatg
     3001 cttctgcaac ttcgtacagg aataaaacgc gcaacgctac aagccgcgca tttttcacca
     3061 acgcatgaaa acgtcatggc caatctagcc tctttataca ctgatacgct cagtaccttt
     3121 agctttagga tccacgttac aggcgatcct ctctacctta accaaacgta tcatgtaaat
     3181 aaagtacgtg cactcttatt agcgggtgta cgctcagctg ttttatggca acagttgggc
     3241 ggacgccgct ggcaactcct cctttctcgc accagtattt tgcacactgc ccacgaatgg
     3301 ctaaatgaac ttatcactga agaaacagtc tgacttagta tgagtttcgt catcagtgag
     3361 gtaagtgcat gtccccaatg tggtctgatt tatggaaaga cttaacagag aaatttcaat
     3421 ccattgaaac agcgaaaaac gatttgcttc attcaaatga tctccctcat ccggcagaag
     3481 aaaatgcgcc tcccaaaaac aaaaccttaa ccttgcagtt agatagagag aaagcagata
     3541 atttctctca acaaatactc caactgtcta aagaaccaaa aggtggtccc tcctcaaaac
     3601 caaacgatta atttatctat tcc
//
LOCUS       DAORVT010000105         3608 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_21163_length_3608_cov_4.886012, whole genome shotgun sequence.
ACCESSION   DAORVT010000105 DAORVT010000000
VERSION     DAORVT010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21163_length_3608_cov_4.886012"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..1540
                     /gene="glnA"
                     /locus_tag="PK389_02880"
     CDS             131..1540
                     /gene="glnA"
                     /locus_tag="PK389_02880"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HQY22688.1"
                     /translation="MVVADVLKLIQDNEIKFVDLRFTDTNGKEHHVTVPAHTFDADKF
                     EDGHPFDGSSIGGWKSIQASDMLLIPDPDSANMDPFMDEPTLLMTCDVVEPADGKGYS
                     RDPRSLAKRGEIYLKSTGIGDVAYFGPEPEFFIFDSVRWHADMSGCSVKIESEEAAWS
                     SAIKYESGNIGHRPAIKGGYFPVPPVDSLQDMRSAMCLTLEEMGIGVEVHHHEVATAG
                     QCEIGTRFNSLVKRADWNQILKYVVHNVAHSYGKTATFMPKPIVGDAGSGMHVHQSIW
                     KDGKNLFSGNGYAGLSEMALFYIGGILKHAKALNAICNPSTNSYKRLVAGFEAPVNLA
                     YSASNRSAAIRIPHTSSPKGRRIEVRFPDPTANPYLAFTALMMAGLDGIQNKIHPGDP
                     MDKNLYDLPPEEAAKVPNACASLNEALECLDKDRDFLVRGGVFTNDVIDSFIRLKMDE
                     VTLLRMTTHPVEFDMYYSL"
     gene            1787..2230
                     /locus_tag="PK389_02885"
     CDS             1787..2230
                     /locus_tag="PK389_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HQY22689.1"
                     /translation="MKISTLIVLLALISLGSLSNATAGTDIYQHVDKEGNITFTNRNI
                     KNAQKISIASFSRNSESTHSKLSSNPSHGKDVKQKERDAMRRHILEKELLTEKKLLSD
                     TQNFIEQINHNPQLSNSQEAVIQLKNKFFLHQRNITALKKELAKL"
     gene            2270..2728
                     /locus_tag="PK389_02890"
     CDS             2270..2728
                     /locus_tag="PK389_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HQY22690.1"
                     /translation="MRKANHALLIVLACLPCTTHSGVYKHIDEHGNVTYSNISLPNSK
                     KVDLPPIVVVPSVDTTDIEERLATRRENSKIDEQRALIENKISEEEKHLNEIKNEYKD
                     GVPDRLGSERNYQRYLNRVERLKEEINVRENNLNVFKKELQNLPVKTKSR"
     gene            complement(2731..3498)
                     /gene="xth"
                     /locus_tag="PK389_02895"
     CDS             complement(2731..3498)
                     /gene="xth"
                     /locus_tag="PK389_02895"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HQY22691.1"
                     /translation="MKLATWNVNSLKVRLPQVIDWLDINRPDVLCLQETKLQDENFPE
                     KEIAAVGYQSVFIGQKTYNGVAILSKQKGAEIITAIPGFLDDQKRVVAATYDDVRVIS
                     IYVPNGDTVESEKYIYKLKWLPALSDWLQEEIKQYPKLAILGDFNIAPEDRDVYDPQH
                     WEGRVLCSQPERAAFSKLLHLGLVDSFRLFEQPEKSYTWWDYRMMAFRLNRGLRIDHI
                     LLTNELASYCKTCLIDKTTRKLERPSDHVPVMVELSK"
BASE COUNT         1160 a          742 c          651 g         1055 t
ORIGIN      
        1 gcgtattgat tttccaacca atttgttaat atcgattcaa attattcatt gtgctctgct
       61 ggtataattc tatctaaatg tatctttatt ttgttttact aaatttgtaa cgctagtctg
      121 gagagaaaaa atggtggtag ctgatgtttt gaaattgatt caagacaatg aaatcaagtt
      181 tgtcgattta cgttttaccg ataccaatgg aaaagaacat catgtaaccg ttcctgcgca
      241 tacttttgat gcggacaaat ttgaagatgg acatcctttt gatggctcat ctattggcgg
      301 ctggaaaagc attcaagcat ccgacatgct gttgatcccc gatccggact ctgccaacat
      361 ggatcccttc atggatgaac caacattgct gatgacctgc gatgtggtcg agccagcaga
      421 tggcaaaggc tatagccgcg atccacgttc gttagccaaa cggggtgaaa tttacttaaa
      481 atcgactggt atcggtgatg tggcttattt cggtccagaa cctgaatttt ttatttttga
      541 ctcggtccgt tggcatgccg atatgtcggg ctgctcggtt aaaattgaat cagaagaagc
      601 agcttggagt tcggcgatca aatacgaaag tggcaatatc ggtcaccgcc ccgccataaa
      661 aggcggctac ttcccggttc ctccggttga ttcactgcaa gacatgcgtt ctgccatgtg
      721 ccttaccctg gaagaaatgg gcattggtgt cgaagtgcat catcatgaag tcgctaccgc
      781 aggtcaatgt gaaattggca cacgctttaa cagcttggtt aaacgtgcag attggaacca
      841 aatccttaaa tacgtcgtac acaatgtagc acattcgtac ggcaaaacgg caactttcat
      901 gcccaaaccg attgtcggcg atgcgggctc aggtatgcat gtgcatcaat ccatctggaa
      961 agatggcaaa aacttatttt ctggaaacgg gtacgcgggt ttatcagaaa tggcactttt
     1021 ttatattggg ggcatcctca aacatgcaaa agcgctcaat gccatttgca atcccagcac
     1081 caattcatac aaacggctgg ttgccggatt tgaagcacct gtcaatcttg cttattcagc
     1141 aagtaatcgc tcggctgcaa ttcgcattcc acacaccagc agtcccaaag gccgacgaat
     1201 tgaagtgcgc tttcctgacc caaccgcgaa tccgtattta gcatttactg cactaatgat
     1261 ggccggcttg gatggtattc agaacaaaat acaccccggt gatccgatgg ataaaaatct
     1321 ctatgatctt cctccagaag aagcagccaa agtgcctaat gcttgcgctt cattaaatga
     1381 agctttggaa tgtttggata aagatcgtga ttttctagtt cgtggcggcg tttttacaaa
     1441 tgacgtgatt gattctttta ttcgtcttaa gatggatgaa gttaccttac tacgcatgac
     1501 gacacatccg gttgaatttg acatgtatta cagtctatag gtacaaggcg aactgaacat
     1561 ccacctcata aaggtaataa aaaattaata ccagaaatac actctattat ggcaatcaat
     1621 aatttttcag agactaatat acaaaactaa attttttgta tagaaacaca aaaattattt
     1681 taattaaaag gatttattgg ttctattttt tcttttcctt tgaaaaatta ttgataaact
     1741 aattcaattg gttgctaatg ctatcagaat cttttctata aaaaatatga aaataagcac
     1801 cttaatagtt ctattagctt taatttcatt gggttcctta tctaatgcca cagcaggcac
     1861 cgacatatat cagcatgtcg ataaagaagg aaatattacc tttaccaatc gtaatattaa
     1921 aaatgctcaa aaaatctcta ttgcatcctt ttccaggaac tctgaatcaa ctcattcaaa
     1981 attatcttca aatccttctc atggaaaaga tgtaaaacag aaagagcgag atgctatgcg
     2041 ccgccatatt ctggaaaaag agttacttac cgagaagaaa ctgttaagcg acacacagaa
     2101 tttcatcgaa caaattaatc ataatcctca attaagtaac tctcaagaag ctgtcataca
     2161 attaaaaaat aaatttttcc tacaccaaag aaatataacc gcattaaaaa aagaactcgc
     2221 taagttataa gtatcttctg cagtgcataa atgatcaaaa tggtgaatca tgagaaaagc
     2281 aaatcacgct ctacttatcg ttttagcttg tttaccatgc acaacccatt ctggcgtcta
     2341 caaacatatc gatgaacatg gaaatgtaac gtactcaaat atatctcttc caaattcaaa
     2401 aaaagttgac ctgcctccta ttgttgttgt tccgtcggta gacactacag atattgaaga
     2461 aagactagct acaagaagag aaaattccaa aattgatgag caacgtgcat taattgaaaa
     2521 taagatatct gaagaagaaa aacatttgaa tgagataaaa aatgaataca aagatggtgt
     2581 tcctgatcgg ctgggaagtg aacgcaacta tcagagatac cttaatcgag tcgaacgatt
     2641 aaaagaagaa ataaatgtgc gagaaaacaa tctgaacgtt tttaaaaaag aacttcaaaa
     2701 tttacctgtc aaaactaaaa gcagataatc ttatttagat aattcaacca tcacaggcac
     2761 atgatcagaa ggacgctcta acttacgagt cgttttatct atcaaacatg tcttacaata
     2821 actcgcaagt tcgttggtca ataaaatatg atcgatgcgc aaccctcgat ttaggcgaaa
     2881 agccatcata cgataatccc accaagtata agatttttcg ggttgctcaa ataaacgaaa
     2941 gctatcaact aatcctagat gcagcaattt actgaatgct gctctttcag gttgactgca
     3001 taacaccctc ccctcccaat gctgtggatc atagacgtcg cgatcttcag gggcaatatt
     3061 aaaatcacct aatattgcta gctttggata ctgttttatt tcttcctgca gccaatcact
     3121 caaagctggc aaccacttta atttataaat atatttctca gactcaacag tatcaccgtt
     3181 aggaacataa atagaaataa cccttacatc atcatatgtg gcggcaacca ccctcttctg
     3241 atcatctaaa aatcccggta tggcagttat tatctcagcg cctttctgct tactgagtat
     3301 ggcaacaccg ttatatgttt tctgtccaat aaaaactgat tgataaccaa ctgctgcaat
     3361 ttctttctcc gggaaattct catcttgcag ttttgtttcc tgcaaacaca gcacatcagg
     3421 ccgattaata tctaaccaat caataacttg tggaagcctc actttaagag agtttacgtt
     3481 ccaagttgct aatttcatat ccatctaata attcattatg caatttaacc aatgactgat
     3541 atctgctatc tcttgtttgc ataccgtatg ttccataggg tattcatgcc attcgagtaa
     3601 gtagtctg
//
LOCUS       DAORVT010000106         3581 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_21444_length_3581_cov_3.845718, whole genome shotgun sequence.
ACCESSION   DAORVT010000106 DAORVT010000000
VERSION     DAORVT010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21444_length_3581_cov_3.845718"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2879
                     /gene="rpoC"
                     /locus_tag="PK389_02900"
     CDS             <1..2879
                     /gene="rpoC"
                     /locus_tag="PK389_02900"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011758329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HQY22692.1"
                     /translation="DVVGVKGGANDADFDGDQMAVHIPLTIEAQLEARALMMSTNNIL
                     SPANGEPIIVPSQDIVLGLYYLTRERINVLGEGKVFLDIGEVHRAYQNGAVHLHAIIQ
                     VRVNAEMDSTEAVRAKGYSMVQTTVGRALLSEILPAGMSYALINKTMTKKVISQLINY
                     CYRQVGLKETVIFADRLMYTGFSYATRSGISIGIDDLVIPSSKGSIIAQAEEEVKEIG
                     NQYTSGLVTHGERYNKVIDIWSRTNDQVAKAMMSELASEMVVNAKNETVKQESYNPIF
                     MMADSGARGSAAQMRQLAGMRGLMAKPDGSIIETPIRANFREGLNVLEYFISTHGARK
                     GLSDTALKTANSGYLTRRLVDVANDVVVMELDCGTTNGLTMTPLIEGGDVVEALRERV
                     LGRVVLQDVIDPKTNEIVIENGSLLDEAMVDVLEQIGVDQVKVRSAITCQTRFGICQK
                     CYGRDLSCGQLVNIGEAVGVIAAQSIGEPGTQLTMRTFHIGGAASRATADNSIQVKSS
                     GKVRLNNVKLLMHAKGYSVVVSRSGELTVADAQGREWERYKLPYGAIVTVVDGAEVEA
                     SQTVATWDPHNHPVITEVAGRIRYVDLIDGVTMRRQTDDVTGLTSVVVMDPKQRTSGG
                     RELRPMVKLVDNEENDLMLAGTNLFAHYFLSADSIISAEDGSFVGVGDVIARIPQETS
                     KTRDITGGLPRVADLFEARRPKEAEILAEISGVVSFGKETKGKRRLMITSPDTQTAVH
                     EELIPKWRHVSVFEGEHVQRGEKIADGSPDPHEILRLLGVDALANYIVNEVQDVYRLQ
                     GVKINDKHIEVIVRQMLRKVIVTSTGDTRFLVGEQVDIGDLKTANEKLAAEKMPALYG
                     PVLLGITKASLATESFISAASFQETTRVLTEAAVSGKRDNLRGLKENVTVGRLIPAGT
                     GLAYHEERRRAKLLAKQPDTQPKPPITASDIEQALGEALNSTGT"
     gene            2886..3392
                     /locus_tag="PK389_02905"
     CDS             2886..3392
                     /locus_tag="PK389_02905"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY22693.1"
                     /translation="MSNMIETTRLILRPLCAADFEALCILDGDPEVRSYFPEGVLNSA
                     EVQQELERYLKAWNTDGFGLFAAVDKKSNQLIGRTGFAKLSSGEVEFGYLFLPSYWGR
                     GYATEASQALLAWAAEHIPVDHIIGFAPTHHIASLRVLEKCGMKFFRMDSYRNIECAF
                     YKFDLARN"
BASE COUNT          983 a          641 c          941 g         1016 t
ORIGIN      
        1 tggacgtcgt tggtgtaaag ggcggagcga acgatgccga tttcgacggc gatcaaatgg
       61 ctgtacatat tcctctaacc attgaggcgc agctagaagc acgcgcatta atgatgtcta
      121 caaacaatat tttatcgcct gcgaatggtg agcctatcat tgttccttca caggatattg
      181 tgttgggtct ttattatttg actcgcgagc gaatcaatgt attgggcgaa ggaaaagtct
      241 ttcttgatat aggtgaagtg catagagcgt atcaaaatgg cgctgtgcat ttgcatgcaa
      301 ttattcaagt acgtgttaat gcggaaatgg atagcacaga ggcggttcgt gcaaaaggat
      361 atagcatggt gcaaacgact gtgggtcgtg cattattgtc tgaaattttg cctgcaggaa
      421 tgtcttatgc gctgattaac aaaacgatga ctaagaaagt gatttcacag ctgataaact
      481 attgttatag acaagtcggc ttgaaagaaa cggttatttt tgcagatcgg ttaatgtata
      541 ccggcttctc ttatgccaca cgttcgggaa tttctattgg cattgatgat ttggtgatcc
      601 cttccagtaa gggcagcatt attgcgcaag cagaagaaga agtgaaagaa attgggaatc
      661 aatatacctc tggtttagtg acacatggtg aacgttataa caaagttatt gatatttggt
      721 ctcgcaccaa tgatcaagtt gctaaggcta tgatgtcgga attagcctcg gaaatggttg
      781 taaatgcaaa aaatgaaacc gtcaaacaag agtcttataa cccaatcttt atgatggcag
      841 attcgggtgc tagaggttct gctgcgcaaa tgcgacagtt ggcgggtatg cgtggtctta
      901 tggctaaacc ggatgggtcg attatcgaga cgcctattcg cgctaacttc cgtgaaggtc
      961 ttaatgtatt ggaatacttt atttcaaccc atggtgcacg taaagggttg tcggatactg
     1021 cattaaaaac agcgaattct ggatatctca cacgtcgatt ggttgacgtt gctaacgatg
     1081 tggtggttat ggagctggat tgtggtacta ccaatggttt gaccatgacg ccgttgattg
     1141 aaggtgggga tgttgtagaa gcgttgcgtg aaagggtatt aggacgagtt gtattacagg
     1201 atgttattga tcccaaaacc aatgaaatcg tcattgaaaa tggcagttta ttagatgaag
     1261 ccatggtcga tgtgttagag cagattggcg tagatcaagt caaagtacgt tcggcgatta
     1321 cttgccagac acgctttggt atttgccaaa aatgttatgg gcgtgattta tcttgtggtc
     1381 agttggttaa tatcggggaa gctgtcgggg ttattgcggc gcagtctatt ggtgagccgg
     1441 gaacacagtt aaccatgcgt actttccata ttgggggtgc tgcatcgaga gcgactgcag
     1501 ataatagtat tcaagtgaaa tctagcggca aagtgcgttt aaacaacgtg aaattgttga
     1561 tgcatgctaa agggtattcc gttgtggtct ctcgttcagg agagctgacc gttgccgatg
     1621 cgcaaggtcg tgagtgggag cgatataaat taccttacgg tgctattgtg acggttgtcg
     1681 atggtgcaga agtcgaggca tcgcaaacag tggcgacatg ggatcctcat aatcaccctg
     1741 ttataacaga ggttgctggt cgcatacggt atgtcgattt aatcgatggt gtaacgatgc
     1801 gtcgtcaaac agacgatgtt acagggttaa ccagtgttgt tgtaatggat ccaaaacaac
     1861 gtactagcgg tggacgtgag ttacggccga tggtaaaact tgtagacaat gaagaaaatg
     1921 atttgatgtt agcaggtacc aatttatttg cgcattactt cttatctgcg gattcaatta
     1981 ttagtgctga agatggttct tttgtgggtg tgggcgacgt tattgcacgt attccgcaag
     2041 aaacctccaa gacacgtgat atcacaggtg gtttacctag agttgctgat ctcttcgaag
     2101 cgcgtcgtcc gaaggaagct gaaattttag cggaaatctc gggtgtcgtt agtttcggaa
     2161 aagaaactaa aggtaagcgt cgtttgatga ttacttcacc cgatacacaa acggcggtgc
     2221 atgaagaatt gattccaaaa tggcgtcatg taagcgtgtt tgaaggtgag catgtgcaac
     2281 gcggtgaaaa aatagccgat ggttcgccag atccacacga gatattacga ttgctgggtg
     2341 tggatgcctt agcgaattat atagtcaacg aagtgcaaga cgtgtatcgc ttacaagggg
     2401 ttaagatcaa cgacaaacat atcgaagtga ttgttcgtca aatgttacgt aaagtgattg
     2461 tgacgtctac aggagatacg cgctttctcg tgggtgagca agtagatatt ggtgacttaa
     2521 agacagcgaa tgagaaactc gcagcagaaa aaatgccagc gctttatggt cctgtattat
     2581 tgggtattac aaaagcttca ttggcgactg agtcctttat ttccgcggct tctttccaag
     2641 aaacaacgcg tgtattaacc gaagcggctg taagtggtaa acgcgataat ttgcgtggat
     2701 taaaagagaa cgttaccgtc ggccgattga tcccagcggg aaccggcttg gcgtatcatg
     2761 aagaacgtcg tcgggctaag ttgttggcca agcagccaga tactcaaccg aagccaccaa
     2821 ttacggcgag tgatattgag caagcattgg gagaagcgct gaattctacg ggaacataaa
     2881 gttgtatgtc caatatgatc gaaaccacgc ggttgatctt acggccttta tgcgctgcag
     2941 attttgaagc attatgtatt ttggatggcg atccagaggt tcgcagttat tttccagaag
     3001 gcgttttaaa tagcgcggaa gtccaacaag aattggagcg ctatctaaaa gcctggaata
     3061 cagatggctt tggtctattt gcagcggtag acaaaaaaag caatcaactg attggccgta
     3121 cgggttttgc aaaattaagt tctggtgaag tggaatttgg gtatttgttt ttgccatcgt
     3181 attggggtag aggctatgcc actgaagcaa gtcaggcact gcttgcgtgg gcggctgaac
     3241 atattccggt tgatcatatt attggttttg cgcctacgca tcatatcgct tcgcttcgcg
     3301 tactagaaaa gtgcgggatg aaatttttta gaatggacag ttatagaaat atcgaatgtg
     3361 cgttttataa attcgatctt gcaagaaatt gatgctttgt ggaaacctta gttcaacagt
     3421 aatttccttt tctagaaatg tggtgcttcg cacgctctat aactgccgat tttgtcgaag
     3481 ttacgctcac taaatccagc tgccatcctc gtacacgttg atatgcaagt attttataat
     3541 aggtcttttg cttgccctga gccatcagca gagaaaccgc a
//
LOCUS       DAORVT010000107         3551 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_21775_length_3551_cov_5.302059, whole genome shotgun sequence.
ACCESSION   DAORVT010000107 DAORVT010000000
VERSION     DAORVT010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21775_length_3551_cov_5.302059"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2829
                     /locus_tag="PK389_02910"
     CDS             <1..2829
                     /locus_tag="PK389_02910"
                     /inference="COORDINATES: protein motif:HMM:NF013676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HQY22694.1"
                     /translation="HYLPNDNTPKQLPTRKIQRKTKEKILQLMGFDDNLDKTDQLILE
                     KASEIVKTTQNPLDIFESILKQLESAKRVLPKYSRLQDIIGMALKNEDQRLIKMVKHH
                     LTKSVRGALQNLFQSDDAFYHITELKFDAKSFQTKEMTAEIKKLDLCRPIYDFTKDFL
                     PKLSLSRHMVEHYSDLAKLYSVWRLQNIPPELSYLYLICYVHGRYERLTNNLIQAFVY
                     YVDKYQNEAKDYAKHHWAILKNPLEQHQTAIGKLMGIFTDKAIMKLSGNKIEKHAFDI
                     MPEERITTVSKRLLEGGKDRIFEERRLMWEYHKSNYQSLLINLRPLFMVIDFEGNPDL
                     KDLFKAITFLKSSFQKKELLNKLPLNLIPIKHIKPKLLLDLFIKKVKAKKSGFKKVIN
                     PYQYEFHVYRTIRDHIKSSKIYINTSIGYKSFEAEIKMSPNWQKEKKRILKDLNNPIL
                     LRPIQETLAELEGILEPLIVRTNKRALNGDNKHIHITHHRDGRVDWTIPYPKRNKEAD
                     NPFYDQLDTKTISEIFDFVEQKCGFMKAFIHIKPKGAKSKKDYLGIKATILANGTMQG
                     THLFSKRSNLKYQRLQTAEQNHVRLATLQKAAEIIVNHMINLPIFDLYDLGGKKHGSI
                     DGTKKKTRRRILRARHSKKYFGGDIGVVVMSMILGHVPFVSKIIGANEHESHYTYPML
                     HNNSTEIDPDIISTDTAGTNNVNDFMYYLIGKLHAPCYRSTAKKTKTLCGFKSLSHYK
                     NLLIKPSRAVNRKRITQKWPELIPVLVSLLSHNTTQENILKILSSHEYKSDVKEAFWE
                     LNQILKSIHLLKDIDDPKYRRNIRTALNRGEAYHQLLGKIMSVGGGDFRGMSDLEVEI
                     WNECARLLALIIIYYNMHLLSKLYEVALAKKDKAVLKFLKHISPVASQHINISGLYEF
                     SEIISTINVDGVVAILSRILKDVVYHQK"
     gene            complement(3059..>3551)
                     /locus_tag="PK389_02915"
     CDS             complement(3059..>3551)
                     /locus_tag="PK389_02915"
                     /inference="COORDINATES: protein motif:HMM:NF016433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22695.1"
                     /translation="EILRIVATYPASLVPMFQAFRAGDKGRHKIQSLIYGLVDLMPIK
                     TAASATLDNPDGSEGFNLTEIKKRLDALLLLRFKVDRALSIQESLQNPEAIKAIEKLS
                     QSLTGFKWTPRALMLLSSSLKGLLVQISSVEDAIMRLCITGFYAYAPKMFPAPIAHLT
                     FSR"
BASE COUNT         1209 a          699 c          685 g          958 t
ORIGIN      
        1 cactaccttc cgaatgacaa tacccccaag caattgccga cacgtaaaat acagcgcaag
       61 acaaaagaaa aaatattaca gctcatgggg tttgatgata accttgataa gactgatcag
      121 ctcattctag aaaaagccag tgaaattgtc aaaaccacac aaaatccttt agacatattt
      181 gaatcaattc taaaacaact cgaaagcgcc aagagggtat tacccaaata ttcccgatta
      241 caagacatca tcggcatggc acttaaaaac gaagatcagc ggcttattaa aatggtaaaa
      301 caccatctta ctaaaagtgt tcgcggagcc ttacaaaact tatttcagtc ggatgacgca
      361 ttttatcata tcacagagct taaatttgat gccaaaagtt ttcagacaaa ggaaatgacc
      421 gcagaaatta aaaagcttga cctttgcaga ccgatctatg attttacaaa ggactttttg
      481 cctaagctat cgttatctcg tcacatggta gagcattatt ctgatttggc caaactgtat
      541 agtgtttgga ggttacagaa cattccaccg gagttgtctt atttgtacct aatttgttat
      601 gtgcatggtc gatacgaacg attgaccaat aatctcatcc aggcatttgt ttattacgtc
      661 gataaatatc aaaacgaggc caaagattat gcgaaacatc attgggcgat tctaaaaaac
      721 ccgctggaac aacatcaaac cgctatcgga aaactgatgg gtatttttac ggacaaagct
      781 atcatgaagc tatctggcaa caaaattgaa aagcatgcat ttgatatcat gcctgaagaa
      841 cgtattacaa cagttagcaa aagactctta gaaggaggta aggatcggat ttttgaagaa
      901 cgaaggctga tgtgggagta tcacaaaagc aattatcaat ccctcttaat caatttgcgt
      961 ccattgttta tggtaattga ttttgaggga aatcccgatt taaaagatct ttttaaggcc
     1021 attacatttt taaagtcttc atttcaaaaa aaagagttgc ttaataagct tccattaaat
     1081 cttataccca tcaaacacat caaaccaaaa ttgttgttag atttattcat caaaaaagtg
     1141 aaggctaaaa aatccggatt caaaaaagtc atcaatcctt atcagtacga atttcatgtt
     1201 tatcgcacca ttcgagacca catcaaaagc agtaaaattt acatcaatac ctctattggc
     1261 tacaaaagct ttgaagctga gattaaaatg tctcctaact ggcagaaaga gaaaaaaaga
     1321 atattgaaag atcttaataa ccccatcttg ttaagaccca ttcaggaaac acttgccgaa
     1381 ctagaaggta tcctggaacc cttaattgta cgcaccaata aacgggcatt aaacggtgac
     1441 aataagcaca tccatattac acatcatcgc gatggacggg tggattggac aatcccttac
     1501 ccaaagcgaa acaaagaggc tgacaatcca ttttacgacc aattggacac taaaacgatt
     1561 tctgagatat ttgattttgt agaacagaaa tgtggtttta tgaaagcctt tatacacatt
     1621 aagcccaaag gagccaagag taaaaaagat tacctgggta tcaaagcaac catactggcg
     1681 aatggcacca tgcaaggtac ccatttgttc tccaagagat ccaatctaaa atatcaacga
     1741 cttcaaacag cagaacaaaa tcatgtgcga ttggcaactt tacaaaaggc tgctgaaata
     1801 atcgtaaatc atatgattaa ccttcccatc tttgatctct acgatttggg cggaaagaaa
     1861 catggcagta ttgatggcac taagaaaaag acacggcgtc gtattctaag agcacggcat
     1921 tctaaaaaat acttcggtgg tgatatcggt gttgtcgtga tgagcatgat tttgggacat
     1981 gtgccctttg tgagcaagat cattggcgcc aacgaacacg agagtcatta tacttatccg
     2041 atgttacata ataatagcac cgagattgat cctgacatca tttcaacaga cacagcgggt
     2101 accaataatg tcaatgattt tatgtattat ttaattggaa agctacatgc gccttgttac
     2161 cgatcaaccg ccaagaaaac caaaaccctc tgtgggttca agtcgctctc tcattacaaa
     2221 aatttactga ttaaaccctc ccgggcagtg aacagaaagc gcattactca gaaatggcca
     2281 gagcttattc ccgtactggt atcgctatta tcacacaata ccactcagga aaacatcctt
     2341 aaaatcttaa gttcacatga gtataaaagt gatgttaaag aggctttttg ggaactgaat
     2401 cagatactca aaagcatcca tctcttaaaa gatatcgatg atcccaagta tcgacggaac
     2461 attcgtactg cactcaatcg gggtgaagcg taccatcaat tgcttgggaa aattatgtca
     2521 gttggtggtg gagattttag aggtatgtct gatttagaag tagaaatttg gaatgaatgt
     2581 gccagactgc ttgcgcttat cattatttat tacaatatgc acttgctgtc aaagctgtat
     2641 gaagtggcgc tggcaaaaaa ggataaagca gtgttaaaat ttttaaagca tatttcaccc
     2701 gttgcctctc agcacatcaa tatcagcggt ctctacgaat ttagtgaaat tattagcacg
     2761 attaacgttg atggggttgt agcaatctta agcaggattc taaaagatgt cgtatatcat
     2821 cagaaatgaa tctatccagg ctatagttct gcaatatttc aataaaagcg ccctaaacta
     2881 ttgcgatttt aaaataaaac cgttaccctt gcaggcaagg gctgtgaatg atgcagaaaa
     2941 ccaaaatatg aatctccggt aaaactcttt aaaactaggc taaacttcaa atctacgaag
     3001 cacaaatggg aaaatttgcg ctaaatggcg agagataata aaggcataaa atcgcgtttt
     3061 atcgagaaaa agtgaggtga gctatagggg ctgggaacat tttcggggca tatgcataga
     3121 accccgtaat gcaaagtcgc ataatagcgt cctctacaga actaatttgc actaaaagac
     3181 cctttaagct actacttaaa agcattaatg cacgcggtgt ccatttaaag ccagtcaaac
     3241 tttgactgag tttctcgatg gcttttatag cctccggatt ttgtaggctc tcttggatgg
     3301 ataaagccct gtccactttg aatctcaaga gcaataaagc atccaggcgc tttttaattt
     3361 cggttaagtt aaacccttca gacccatcag gattgtctaa ggtggcggat gctgcggttt
     3421 ttatgggcat taaatccact agcccataga tgagcgactg gattttgtgg cgacccttgt
     3481 cgcccgccct aaacgcttga aacatgggaa caagagaagc gggataagtg gcaacgatgc
     3541 ggagtatttc g
//
LOCUS       DAORVT010000108         3524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_22087_length_3524_cov_3.287114, whole genome shotgun sequence.
ACCESSION   DAORVT010000108 DAORVT010000000
VERSION     DAORVT010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22087_length_3524_cov_3.287114"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PK389_02920"
     CDS             <1..231
                     /locus_tag="PK389_02920"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010317646.1"
                     /note="poxA; regulates pyruvate oxidase poxB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P lysine(34) lysyltransferase"
                     /protein_id="HQY22696.1"
                     /translation="HELTDPDIQLERIQADCEYRKTNGLMEIPIDFRLIEALKAGLPD
                     SAGVALGLDRLLMLMLSSHEIQEVVSFTTDRA"
     gene            complement(212..1060)
                     /gene="asd"
                     /locus_tag="PK389_02925"
     CDS             complement(212..1060)
                     /gene="asd"
                     /locus_tag="PK389_02925"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006034772.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HQY22697.1"
                     /translation="MLSRLQRFLPQHCLSSFAGLLANCKIRWLKNRLIQYFVWRYPIN
                     LAEALNPNPYEYDCFNDFFTRALKLERRPIASGIHDLVSPVDGQISQLGAIAAGKIFQ
                     AKGHHFTTEELLGGNTALAEPFTNGSFLTVYLAPKDYHRVHMPIDGALTQMIYVPGSL
                     YSVNFKTTGCTPNLFARNERVIAIFNTSIGRVAVVLVGAMIVGSIEMISTGTITPPRC
                     KSIQIWKLDHPIHLKRGEELGRFKLGSTVIMLFEENKVSWDPVFTTTELVQFGQRLGS
                     FKPDQW"
     gene            complement(1136..1366)
                     /locus_tag="PK389_02930"
     CDS             complement(1136..1366)
                     /locus_tag="PK389_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22698.1"
                     /translation="MDTDLDKDITMRVRSLVEPSLETLQEYFETLVLKREITTPLQYL
                     SETHRRGIANKFAKKSLQELEWMARKLDKSLL"
     gene            1566..2114
                     /gene="orn"
                     /locus_tag="PK389_02935"
     CDS             1566..2114
                     /gene="orn"
                     /locus_tag="PK389_02935"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HQY22699.1"
                     /translation="MKPNPLHLIWIDLEMTGLNPDEDRIIEIATVITDRNLEIIAEGP
                     VFAIAQSEKRLLAMDEWNTKQHTRSGLVERVRNSPMNEEDAEAQTLEFIMRFVPPNRS
                     PMCGNSICQDRRFLYRHMPKLEQYFHYRNLDVSTVKELAKHWKPSIVKLFKKKSMHLA
                     MEDIKESIGELRHYRDTFFLLE"
     gene            complement(2121..3293)
                     /locus_tag="PK389_02940"
     CDS             complement(2121..3293)
                     /locus_tag="PK389_02940"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQY22700.1"
                     /translation="MLSDESLLEILSEWSFWDAEPPSGLTRQLALPKALQADLALIIQ
                     GVRRCGKSTLLSQLPKHYHIPLAQCYYCNFEDPRLMNDLDHKLLTRIKTLARNKIPAE
                     KPCYFFFDEIQNVQDWEKWIHTQLERPKQNHFILTGSNSRLLSGEFGTALTGRHITLE
                     LFPFLFAEFKALFPNKTLEDYLISGGFPRPLTFDQPYQLLQEYFNDIILRDVLKRVHA
                     RTPDAIKQVAKMAFETCGSELSYRKIAAVTGLTVDTVKAYLEACESAYLLFACNYFAF
                     SEKKQLSKQKKYYPIDSGMRFSTTNTMNRDLGKSLEMMIFLRLKKTHDQVFYWSELHK
                     GEVDFVAVRGKTIVPYQVTWNGSEPRHEKALQHFYENFPQASEAVFITRDNAEDFL"
BASE COUNT         1027 a          650 c          752 g         1095 t
ORIGIN      
        1 catgaattaa cagatccgga tattcaactt gaacgcatac aagcggattg tgaatatcga
       61 aaaaccaatg ggttaatgga gatcccgatt gattttcgtt taattgaggc tttaaaagcc
      121 ggtttgccgg attcagcagg ggtcgcctta ggacttgatc gattattaat gctgatgctt
      181 agtagccacg aaattcaaga agtcgtttca tttaccactg atcgggctta aacgatccca
      241 aacgttggcc aaattgcaca agctctgttg ttgtaaaaac gggatcccag ctgaccttgt
      301 tttcttcaaa caacataatg acggttgaac ccaatttaaa gcgtcccaat tcttcgccgc
      361 gctttaaatg aataggatga tccaatttcc aaatttggat ggatttgcaa cggggaggcg
      421 taatagtgcc tgtagaaatc atttcaatgc tcccgactat cattgcgccg accagtacga
      481 cagcaacacg tccaatggat gtattgaaaa tggcaatgac acgctcattt cgagcaaata
      541 aattgggtgt gcagcctgtc gttttaaaat taacagagta taggctgcct ggtacgtaga
      601 tcatttgtgt taatgcacca tcaatgggca tatggacgcg atgatagtcc ttgggggcta
      661 gatacaccgt gagaaaagat ccatttgtaa agggttctgc gagtgcggta ttcccgccta
      721 atagctcttc tgttgtaaaa tggtgtcctt tggcttgaaa aattttgcct gctgcaatgg
      781 cgccgagttg gctgatttgg ccatcaacgg ggcttacgag atcatgaata ccgcttgcaa
      841 tggggcgtct ctcgagtttt aaagcccgcg taaaaaaatc attaaaacaa tcgtattcat
      901 aggggttggg attaagggct tctgctaagt taatagggta gcgccaaaca aaatattgga
      961 tcaatctatt tttgagccag cgaattttgc aattcgctag taatccggca aaagaggaca
     1021 agcaatgttg aggtagaaaa cgttgtaatc ttgataacaa acaggtgctc cttggcttta
     1081 aatgatatta gtttgaataa ctttcgtcgg ttttataata ccagggagaa gatttttata
     1141 atagagattt gtctagtttt cgagccatcc actctagttc ttgtagcgat tttttagcaa
     1201 atttattagc aatacctcgg cgatgcgttt cacttaagta ttgaagaggt gtggttatct
     1261 ctcgctttaa aacgagcgtt tcaaaatact cttgtagcgt ctctaatgaa ggttctacta
     1321 atgaacgtac gcgcatggta atatccttat ccaaatcagt atccatctgt ctaccctaag
     1381 attgtgttct aagttcgcaa aactaatatt gagagtgtat tttgttctaa aacaggttgt
     1441 caattttttt atgttttttt gccagcgcga gtatttgtgt agttagcttt ggtacttttg
     1501 tgtttaactg taaggtaaca tgaggtataa tcaaattcct ttaatttatg gcgatgagaa
     1561 gattaatgaa accaaaccca ctccacctca tttggatcga tttagaaatg acaggcctta
     1621 atccagatga agaccgcatt atcgaaattg ccactgtcat taccgatcgc aatttagaga
     1681 ttattgctga agggcctgtt tttgcgatcg cgcaatcaga aaaaagactg ctcgccatgg
     1741 atgagtggaa taccaaacag catacacgtt caggtttggt tgaacgcgtc cggaacagcc
     1801 cgatgaatga agaagacgca gaggcgcaaa ccttggaatt tattatgcgt tttgtgccac
     1861 caaatcgctc cccgatgtgt ggcaatagta tttgccaaga tagacgattt ttatatcgac
     1921 atatgccaaa attagaacaa tattttcatt atcgtaactt ggatgtcagc acggtaaaag
     1981 aactggcaaa acattggaag ccaagtattg tcaaattgtt taagaaaaaa tccatgcatc
     2041 ttgcaatgga agatatcaaa gagtctattg gggaattgcg gcattataga gacacttttt
     2101 ttctgcttga ataagggaga tcacaaaaaa tcttctgcat tgtcacgcgt gataaatacc
     2161 gcttcacttg cttgtgggaa attttcgtag aaatgttgta gtgctttttc gtgtcgcggc
     2221 tcagaaccat tccaagtgac ttgatatggt acaatggttt ttccgcgtac tgcaacaaaa
     2281 tctacctcac ctttgtgaag ttcactccaa tagaatactt ggtcatgcgt tttttttaaa
     2341 cgtaaaaaga tcatcatctc tagacttttg cccaagtcac gattcatcgt gtttgttgtc
     2401 gaaaatcgca taccggaatc aatagggtaa tatttttttt gtttgctgag ttgttttttt
     2461 tctgagaatg caaaataatt gcaagcaaaa agcaaatagg cgctttcaca agcctcgaga
     2521 taagctttaa ccgtatctac tgtcaaacct gtgacggcgg caatttttct ataactgagt
     2581 tcagagccgc aggtttcaaa tgccatttta gcaacctgtt taatggcatc tggcgtgcgt
     2641 gcgtgcactc tttttaaaac atctctcaaa ataatatcgt taaagtattc ttgcagtaat
     2701 tgataaggct gatcgaatgt cagtggtctt gggaatcccc ccgaaattaa ataatcttcg
     2761 agcgttttat ttggaaataa tgccttaaat tctgcaaaca aaaaagggaa taattcaagt
     2821 gttatatggc gtccggttaa ggcagttccg aattcaccac tcaaaagacg tgagttagaa
     2881 ccagtcaaga taaagtgatt ttgctttggg cgttcgagtt gcgtatgtat ccatttttcc
     2941 caatcttgta cattttgtat ttcgtcaaaa aagaaataac atggcttttc agcgggaatt
     3001 ttatttcgag cgagtgtttt gatacgcgtt aagagtttat gatcaagatc attcattaat
     3061 cgtggatctt caaaattaca gtagtaacat tgcgccagtg gaatatggta atgctttggt
     3121 aattgcgaga gtaatgttga cttcccacag cggcgtacac cctggataat caatgctaaa
     3181 tcggcttgta gcgcttttgg taatgctaat tgtcttgtta gcccagatgg cggttctgca
     3241 tcccagaagc tccattcgct taggatttct aaaagagact catcagaaag cataaaagat
     3301 cgcctaaagg attagattgg cagtggtgtc aagtatactc gacaaaaact attttgtcaa
     3361 cctaagttga caaaatacat atctgtttga ttttaaacga tattttttta cgcgagcgct
     3421 agcaagcatt ttttaaatat atgtttagca cttttgttat catgggcgga tttgacccca
     3481 ttacctttta ggatctgagg agggtgctgt gggtgtatca gata
//
LOCUS       DAORVT010000109         3501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_22356_length_3501_cov_3.980847, whole genome shotgun sequence.
ACCESSION   DAORVT010000109 DAORVT010000000
VERSION     DAORVT010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22356_length_3501_cov_3.980847"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="PK389_02945"
     CDS             complement(<1..968)
                     /locus_tag="PK389_02945"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010598112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HQY22701.1"
                     /translation="MFYGILDLSWWGYIVATLILTHITIASVTIFLHRAQAHRALVLH
                     PAISHFFRLWLWLTTGMETKAWAAIHRKHHAKCETVEDPHSPQILGLSTVLWGGAELY
                     RKEGKNKETLERYGQGTPDDWLEKNIYTPHSAKGVVLMLAINLFLFGVPGLTIWAVQM
                     AWIPFFAAGIINGVGHYYGYRSFECSDAATNVFPWGLLIGGEELHNNHHTYPASAKLS
                     VKWWEFDIGWLYIRSLMALGLAKVKRTVPKPKIIQGKQFIDAESLHALLNNQVQVMTT
                     YGKAVILPLFRQEKQKALPAERRLLQRVKAALIRADILVDEIGRQN"
     gene            1103..1774
                     /locus_tag="PK389_02950"
     CDS             1103..1774
                     /locus_tag="PK389_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY22702.1"
                     /translation="MQALPLISVENLSYTRPQWAMQDLSFTVNRGECMAFIGKNGTGK
                     TTTLNLIAGLIKPHAGRILIQGFNIHTLPILAKQFIGFLPDQCPLYVELTVREYLSFF
                     AKLRQISKPDIADHVEEALEKLNLKRHQHHLIGILSKGLKQRVGLASAILHRPSVLLL
                     DEPTQGLDQNQIENFQLLLSQYKKNAAIILSTHYLNEVKSICDHALQFGTEEIKTYDF
                     NDCAT"
     gene            1749..2513
                     /locus_tag="PK389_02955"
     CDS             1749..2513
                     /locus_tag="PK389_02955"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQY22703.1"
                     /translation="MILTIARHEFLKLFKTGTIWKLLALCQCVFGLIFYRYMTEFLLK
                     NQNLLIENELSLGATEEIIHPLFAWAALFFFFITPLLATNCLTQEKKSRTLEIYLSAP
                     LTTTKIILGKFFGVFCAQLFLLLPILLMPLLIAVEDSLDFGQFISGSFGLILLLGTNL
                     SIALFIATLSKEPLIASFVIFITLILLSLLEWVTHFLSPAFSWVTEFALLYHCKNFLS
                     GIINTQDILYYALVSFIFLYSSIRCLGKAPYFSQKL"
     gene            2510..>3501
                     /locus_tag="PK389_02960"
     CDS             2510..>3501
                     /locus_tag="PK389_02960"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HQY22704.1"
                     /translation="MKRSIHLKGTFLIFSLFLISTIAYRFPLSLDVTANKINSLSESN
                     RTLLSTLDKPLKVELYSTQQNIIEQVQTILSLFQKESTHVVLSLHQEPLNTLDKTRLR
                     LKTNNNLLLTYDDRKRAIDINPSKWNEAAFSKLIQQIIRVKEDWVVFLSGHGERDPLS
                     DANQDLSQLTHELKTTGINIASLNLGEIGSIPGNTKMLVIADPKVAFLPQEINHILNY
                     VNQGGNLLWLVNPNATPRLDKLAKHLGITWQPGTILDQKSHVMGTPHPAMSILTKYPA
                     HTVTEQLNILTVFPWARPLQYKAASTLGWQASPLLVTNASTTLEVHPTKKAQQTP"
BASE COUNT         1089 a          794 c          600 g         1018 t
ORIGIN      
        1 aaattttgtc gccctatttc atcaaccaat atgtctgcac gaattagggc tgcttttacg
       61 cgctgcaaaa gacgtcgctc tgcaggcaat gctttttgtt tttcttgtct aaataaaggc
      121 aaaatcaccg ctttgccgta ggtagtcatg acttgcactt gattgtttaa caatgcatgc
      181 agtgattcgg catcgataaa ttgttttccc tgtataattt taggtttagg caccgtacgc
      241 ttcacttttg caagccctag agccattaaa cttcgaatat acaaccaacc aatatcaaat
      301 tcccaccact taaccgacag tttagcggaa gccggataag tatggtgatt gttatgcaat
      361 tcttccccac caattaagag tccccaagga aaaacattgg ttgcagcgtc agaacattca
      421 aagctacgat aaccgtaata atgcccaaca ccattgataa tgcctgccgc aaagaaagga
      481 atccaggcca tttgaacagc ccagatggtt aaaccgggga caccgaataa aaagagatta
      541 atggctagca tcaatacgac acctttagct gagtgcggcg tatagatgtt tttttctaac
      601 caatcgtccg gtgttccttg gccataacgc tctaaggttt ctttattttt accttcttta
      661 cggtataact ctgcacctcc ccacaagact gttgacaaac ccagaatttg aggactgtgt
      721 ggatcttcta ccgtttcgca tttagcatgg tgcttacgat gaatagctgc ccatgctttt
      781 gtttccattc cagtcgttaa ccagagccat aaacgaaaaa agtggctaat ggctggatgt
      841 aaaacgagcg ctctatgtgc ttgggcgcga tgcaaaaaaa tagtgacact ggcaatggta
      901 atatgggtaa gaattagggt agcaacaatg taaccccacc atgacaaatc taaaataccg
      961 taaaacatat agagacctct tctgtatagc gattacctgt aaacagttta cgcaaaagag
     1021 aaagtcttta ctagagatgc atccaactaa cataactttg tagaaacgtg cacgttccat
     1081 atccccagct atacttttca atatgcaagc actccccctg atctctgtcg aaaaccttag
     1141 ctatacacgc ccacaatggg cgatgcaaga tttaagcttt actgttaatc gcggcgaatg
     1201 catggcattc attggcaaaa atggtaccgg aaagacaacc acgctcaatc taatcgccgg
     1261 actcattaag ccacatgccg gacgcatttt aatccaaggg tttaatattc acaccttacc
     1321 cattttagcc aaacaattta ttggctttct accggatcaa tgtccactgt atgttgaatt
     1381 aacggttaga gaatatttaa gtttttttgc taaactacga caaatttcaa aacccgacat
     1441 cgctgatcac gtagaagaag cgttggaaaa gcttaatcta aaacgacatc aacatcactt
     1501 aattggcatt ctttctaaag gtctcaaaca aagagtcggt cttgccagtg ccatactgca
     1561 tcgtccttct gtgcttttat tagatgaacc cacacaaggc ttagatcaaa accaaataga
     1621 aaattttcag ctgctcttaa gccaatataa aaaaaatgct gcgattattt tatcaaccca
     1681 ctatttaaac gaagtaaaat ctatttgcga tcacgcgtta caattcggca cggaagagat
     1741 caaaacttat gattttaacg attgcgcgac atgaattcct aaaactattt aaaacaggaa
     1801 caatttggaa gcttcttgct ttatgtcaat gtgtttttgg cttaatcttc tatcgatata
     1861 tgacagagtt tttattaaaa aatcaaaatc ttttaattga gaatgaacta tcgcttgggg
     1921 caacagaaga aattattcac cctttgtttg catgggcagc gctctttttt ttctttataa
     1981 cgccactact tgcgacaaac tgcctcaccc aagaaaaaaa atctcgcact ttagaaatct
     2041 atttaagcgc ccctcttacg accacaaaaa ttattttagg taaatttttc ggcgtttttt
     2101 gcgctcagct ttttttgctg ctgcccattc tcttaatgcc tttattaatt gccgtcgaag
     2161 acagtttaga tttcgggcaa tttatatccg gttcttttgg attaatctta cttttgggta
     2221 ccaacttaag catagcgcta tttatcgcaa cgctctctaa agaaccgctg atcgcatcat
     2281 tcgtaatttt tatcacattg attttgttat cattactaga atgggtaaca cattttcttt
     2341 ctcccgcctt tagctgggtt acggaattcg cattgctata tcactgcaaa aactttttgt
     2401 ctggcattat caatacgcaa gatattctct actatgcctt agtctctttt attttcctat
     2461 attccagtat acgctgctta ggaaaagcac cgtattttag ccaaaaatta tgaaaagaag
     2521 catccatctt aaaggcactt ttttaatttt ttcgctcttt ttaatatcca ccattgctta
     2581 tcgatttcca ctatccttag atgtaactgc aaacaaaatt aatagcctaa gtgaatctaa
     2641 tcgcacatta ctctctacct tagataaacc tctcaaagtc gagctatatt ctacacaaca
     2701 aaatatcatt gaacaggttc aaaccatact ctctttgttc caaaaagaaa gcactcatgt
     2761 tgtacttagt ctacatcaag aaccactgaa caccttagat aaaacccggt taagattaaa
     2821 aaccaataat aacttactgc tgacttatga tgatcgcaaa agagccattg acattaatcc
     2881 ttcaaaatgg aacgaagcgg cgtttagcaa actgatacag cagataatcc gcgtgaaaga
     2941 agactgggtg gtctttttat cgggtcatgg cgaacgcgat cccttgagcg atgcaaatca
     3001 agacttgagc caattaaccc atgaactcaa aaccacgggt atcaatattg cgtcattgaa
     3061 tttaggagaa attggtagca ttccaggcaa taccaaaatg ctcgtcatcg ccgacccaaa
     3121 ggttgccttt ttaccacaag aaatcaatca cattttaaat tatgtcaacc aaggcggaaa
     3181 cttattatgg ctagttaatc caaacgccac acctcgttta gacaaactcg caaagcatct
     3241 cggcattacc tggcaacccg gaaccatttt agatcaaaaa tcgcacgtga tgggcactcc
     3301 tcatccggca atgagtattc tgacaaagta cccagcacac acggtaacag aacagctgaa
     3361 tatcctcacg gtttttccat gggcacgacc attgcaatat aaggctgcct caacgctagg
     3421 ttggcaggca tcccctctac tggtaaccaa tgcttctaca acactagagg tgcatccaac
     3481 aaaaaaagca caacaaaccc c
//
LOCUS       DAORVT010000110         3492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_22440_length_3492_cov_3.725051, whole genome shotgun sequence.
ACCESSION   DAORVT010000110 DAORVT010000000
VERSION     DAORVT010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22440_length_3492_cov_3.725051"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="PK389_02965"
     CDS             <1..970
                     /locus_tag="PK389_02965"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HQY22705.1"
                     /translation="VTALVCHGANPGLITHFAKQAVLDVGNSIKNIESVPQSANEWAN
                     IAQASDIVSLHISERDTQASSIKRIVDEYVNTWSIEGLFEEASENVGFAWGTHEEELP
                     REMVKNRIDGEKCRIIELNQLGVETQINSWVPSKGMFTGFLMPHVEAYSIAELFSRKV
                     LDNRYQPTVHFVYHPCWDAIDSMRHAMAQGWVNINHKRLLGDDILEGKDELGILVVRK
                     HSPDIYWFGSRLDIKEARSLIPHNNATSVQVAVGILSGLVWIIENPQCGIVEPEQVDF
                     KRVLEIAAPYLGEFGGHWAKWPEEHFPKMTGHALKQHFYNSSKEVT"
     gene            complement(1170..1646)
                     /locus_tag="PK389_02970"
                     /pseudo
     CDS             complement(1170..1646)
                     /locus_tag="PK389_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            1609..3063
                     /locus_tag="PK389_02975"
     CDS             1609..3063
                     /locus_tag="PK389_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQY22706.1"
                     /translation="MDFPPPEYPTKPIKDLSLSFPHKTCFDDFNIHIRHGSRIAIIGR
                     NGSGKTTLLKMLQGELEPTSGIIECGTDVVFGCVPQIIENFDSLSGGQRFNEALTHAL
                     CLDPNVLLLDEPTNHLDRQNRTSLMRLLKSYSGTLIVVSHDTELLRYCMDTLWHVDNG
                     QIQVFSGNYDDYICAVHSLRASIEKELIRLERQKKDMHHALMKEQKRAAKSKTQGEKN
                     IHQKKWPTVVSAAKASRAEETSGRKKSVIDNKKQRLNEELAKLRLPEIIVPKFSISAA
                     ELSDRMIISISDGCIGHTGTESLLQKIRLSMRSKERIAIQGDNASGKSTLIKAILGDV
                     SVIKSGNWIVPKRIDIGYLDQHYGTLSAGKTVCETIAERVTTWSYIEVRRLLNDFLFR
                     KNEEVNTEVSRLSGGEKVRLTLAQIAAFTPKLLILDEITNNLDLETRGHVIEVLKNYP
                     GAMIIISHDVDFLKEIGVSDGYEIVDGYFSFSAR"
     gene            complement(3039..3464)
                     /locus_tag="PK389_02980"
     CDS             complement(3039..3464)
                     /locus_tag="PK389_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY22707.1"
                     /translation="MQFKNLKTYDDIAKSFETFLELRPTLSDVQSFTQQILNQQKDGY
                     EIIAIIEDEEVMACIGFRMMTMLAWGKILYIDDLITKEKWQRRGYGKILLEHITKIAK
                     DNQCNQVHLDTGYTRYNAHRIYLNQGFEFHCHHLALKLK"
BASE COUNT         1044 a          647 c          767 g         1034 t
ORIGIN      
        1 ggtcactgcg ctagtttgtc atggcgcaaa cccgggtctt ataacacatt ttgctaaaca
       61 agctgtgtta gatgttggta attccataaa aaatatagag tctgtgccgc agtctgctaa
      121 tgaatgggca aatattgcac aagcgagcga tattgtatcg ttacatattt ctgaaagaga
      181 cacacaagcg agtagcatca aacgtattgt tgatgaatat gttaacacgt ggtctattga
      241 ggggctcttt gaggaagcgt ctgaaaatgt tggctttgct tggggaacgc atgaagaaga
      301 gctgccaaga gaaatggtta aaaatcggat tgatggagaa aaatgtcgaa taattgaact
      361 caatcagttg ggtgtagaaa cgcaaataaa tagctgggtt ccgagtaaag gcatgtttac
      421 aggttttctg atgcctcatg tagaagccta ttctatcgct gaattattta gccgaaaagt
      481 attggataac aggtatcagc ctacagtaca ttttgtttat cacccctgtt gggatgcgat
      541 agattctatg cgtcatgcta tggcccaagg ttgggtaaat ataaatcaca agcgtcttct
      601 aggggatgat attctcgaag gtaaagatga attagggatc ttggttgtgc gaaagcactc
      661 ccccgatatt tattggtttg gatctcggct tgatattaaa gaagcgcgaa gcttaattcc
      721 gcataacaat gcgacttcag tacaggttgc tgttggcata ttgtcgggtc tcgtatggat
      781 cattgaaaat ccgcagtgtg ggatagttga gcccgagcaa gttgatttta agcgggtttt
      841 agaaatagct gctccatatc ttggagaatt tggaggacat tgggcaaaat ggcctgaaga
      901 acatttcccc aaaatgacag ggcacgcttt aaaacaacat ttttacaatt catcaaaaga
      961 agttacttaa ttcagtgtta aatcaacaag ggcggacaca gggatccgct cttgcaaccc
     1021 cattagccct tagataaact ttgtaaccat tttttcttaa gtaatctctg aaaaggatat
     1081 tgcatttttg atacgactgg cctcagcttc aaacatttta ggccaatcga gattgtagga
     1141 aacaacctca atatttttgg tttgtttcat cattcgtcct cactgttgtt atctagcaag
     1201 aatccaccga cctgagcatc ccataaggtt ttatataagc cattcttacc cagaagttct
     1261 gtagcgtgcc atcctctaca attttgcctt gatcaaatac catgatgcga tccatctgca
     1321 gaagcgtgga taggcgatgg gcatcgcaca agctttcttg gatattgctt tcagtcacag
     1381 aatcaagctg tgaggtggct tcatctaaaa tcaaaatcgg ggcatttttt aaaatagcgc
     1441 gagcaacgga tattttccat taaagtacga tgaaaaagcg aagcatcttg tgggatcatg
     1501 gcgatatggc gacgtaggct atcttgggtg atgtcacgaa tatcttggtc atcaatgagg
     1561 atacggccgt cagtcacatc gtaaagtctc agaattaaat tgacgaaagt ggattttccg
     1621 ccgccagaat atccaaccaa acctattaaa gatcttagcc tttcatttcc gcataaaacc
     1681 tgctttgatg attttaatat tcacattagg catggcagtc gtattgctat tattggtaga
     1741 aacggtagcg gaaaaacaac attgcttaaa atgctacagg gtgagttgga acccacaagt
     1801 ggcattattg aatgtggaac tgacgttgta tttggctgtg tgccacaaat tatcgaaaat
     1861 tttgattctt taagtggtgg tcagcgtttc aatgaagctt tgacacatgc cttatgcctt
     1921 gatcctaatg tattgctttt ggatgagccg acgaatcatt tggatcgaca gaatcgaaca
     1981 agcctcatgc gactgctgaa gtcatattct ggtacactca ttgtcgtttc ccatgatacc
     2041 gagctgctcc gttattgtat ggatacttta tggcatgttg ataatggcca aatccaagtc
     2101 ttttctggga attatgatga ctatatttgt gcggtgcaca gccttcgcgc ctctatagag
     2161 aaagaattaa tacgtctgga gaggcaaaag aaagacatgc accatgcctt aatgaaagag
     2221 caaaagcgag ccgctaaaag caaaacccaa ggtgaaaaaa acattcatca aaaaaaatgg
     2281 ccaacggttg ttagtgctgc caaagcgagc agagcagaag aaacatccgg tcgtaaaaaa
     2341 tctgttatcg ataacaaaaa gcaaagatta aacgaagagc ttgcaaagct tcgtcttcct
     2401 gaaattattg tgccaaaatt ttctatcagt gcggcagaac tttctgaccg aatgattata
     2461 tcgattagtg atgggtgcat tgggcatacc ggcacagagt cgctacttca aaaaatacgc
     2521 ctatcaatgc gctcaaaaga gcgtatcgca attcagggcg ataatgcgag tggtaaatca
     2581 acgctaatta aggcaatctt aggggatgtc agcgtgatta aatcgggcaa ttggattgtg
     2641 ccaaagcgta tcgatattgg ttatctggat caacattacg gtaccttatc tgctggaaaa
     2701 acggtatgtg aaacaattgc agagcgagtg acaacttggt cttatattga agtgcgtcgt
     2761 cttttgaacg atttcttatt tcgaaaaaat gaagaggtta acacggaggt gagcaggcta
     2821 tcaggtggcg agaaggtaag gttgacactc gcccaaattg cagcctttac gccaaaacta
     2881 ctgatcttgg acgagataac caacaacctc gatcttgaaa ccagagggca tgtgatcgaa
     2941 gtattaaaaa attatcctgg cgccatgatc atcatttccc atgatgttga ttttttaaaa
     3001 gaaatcggtg tctctgatgg gtatgagatt gtggatggct atttcagttt tagcgctagg
     3061 tgatggcaat gaaattcgaa tccttgattg agatagatcc tatgtgcatt atatctggta
     3121 taccctgtgt caaggtgaac ctggttgcat tgattgtctt ttgcaatttt ggtgatatgt
     3181 tctaataaaa tttttccata cccccttctt tgccattttt cttttgtgat taaatcatca
     3241 atatatagga tttttcccca agcaagcata gtcatcatgc gaaagccgat acatgccata
     3301 acttcctcgt cttcaatgat agcaataatt tcatagccat ctttttgttg attcaaaatt
     3361 tgttgagtaa agctttggac atcagataaa gtcggtctta attctaaaaa tgtctcaaat
     3421 gatttggcaa tgtcgtcgta agtctttaaa tttttaaatt gcataattcc accccacttt
     3481 gtgtttgtaa tt
//
LOCUS       DAORVT010000111         3488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_22489_length_3488_cov_3.383338, whole genome shotgun sequence.
ACCESSION   DAORVT010000111 DAORVT010000000
VERSION     DAORVT010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22489_length_3488_cov_3.383338"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PK389_02985"
     CDS             <1..540
                     /locus_tag="PK389_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQY22708.1"
                     /translation="SGKTTLLQMLGGLDSPTLGEIWMNGHNLHTLPEQELEKVRNQTM
                     GFIYQMHHLLPEFTALENVAMPLLIGNVEPYIAERRAAELLDQVGLKLRLNHRPGALS
                     GGERQRVAIARALVNNPDCVLADEPTGNLDDENAHHVFEVMQALNRTHKTSLIIVTHD
                     SELAAKMDRSYVLHGGHLS"
     gene            593..2965
                     /locus_tag="PK389_02990"
     CDS             593..2965
                     /locus_tag="PK389_02990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HQY22709.1"
                     /translation="MTNTFITSVISFFLGIISLHFFRELPSVALVMIIIVAVGSISWI
                     PQCQKFKPQLFLAYLIGFLWVLLNAKSVVDHRIPDHLEAKPISVVGIIDSIPEHRDNT
                     TRFDFNIQHTLPESLWPNPGRVKLRFRNPTMPLHVGDQLQLTVKLKRPQSYANPGSFD
                     TERHLFQHHMVAEGTVVNKSSRHENLTPTKINDSVLSHPIDRLRMWLLEKINRALKNS
                     EYSGVIDALVVGLQSGITSEQWKVLKDTGTVHLVAIAGLHIAFVTGFVMLIILQIWQR
                     LPRKLYEIIFPKFLKIPVSLLTAVGGLSIAFFYAALAGFSIPTQRAVVVISTFMVSIL
                     SKRHASVWQNYFLALFIVVLLDPLSTLAPGFWLSFGAVGLILYGMKGRLHPKGLWWKW
                     GRIQWVLFLGLSPIMLAIFGMTSLIAPLANFIAIPWVGFLVLPFCLLGTLCVTFSTGL
                     GTFFLDIANGSLSLLFPVLQWLSEFRGATWVNATQPLWSILIGFMGVLLILLPKGFPG
                     KNIGFIFLLPIFLIKPVMVDLNTARVTVLDVGQGLALIVETQGRVLVFDTGPKLNPGF
                     DTGDRVVVPFLATRGRNNIDALVISHGDNDHAGGARSILKAMPVKTIITSEPKLFPEY
                     NTMACFAGQQWAWDGVHFEMLHPTTVMTKKRNDHSCVLKVTAGTQSILLTADIEAKSE
                     HSIVENMPEKLPATVMLVPHHGSRTSSTLEFIRAINPQIAIIPVGYLNQYGHPKPDIV
                     ERYKKENIIVLDTVKQGAISFILNKKNHNNNVDIHAYRQENQRYWHSGGF"
     gene            3031..>3488
                     /locus_tag="PK389_02995"
     CDS             3031..>3488
                     /locus_tag="PK389_02995"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HQY22710.1"
                     /translation="MSVNYLTSAQVYRRLLKAALPYWWAFVLGVLGNALIAASDGWIT
                     YGLKPLIEKGFIARDPIFIRWIPIIVITFFIARGASYFVASYFMTWVGRSVVRDFRRK
                     MVTHLMCLPASFFDQKTSGELLSKINYDADQVAEAISEAITSAIRGVFTTV"
BASE COUNT          958 a          672 c          771 g         1087 t
ORIGIN      
        1 tctggtaaaa caacgctttt acaaatgttg ggtggattag attctcccac cttgggcgaa
       61 atttggatga atggacacaa ccttcatacc ttgcctgagc aggaattaga aaaagtccgt
      121 aatcaaacca tgggttttat ttatcaaatg caccacttgc tacccgaatt taccgccctt
      181 gaaaatgtgg cgatgccttt attaatcggg aatgtagaac cctatattgc agaaagacgc
      241 gcggcggagt tattagatca agtgggctta aaattaaggc taaatcaccg tccaggagcg
      301 ctttcgggcg gcgagcgtca acgtgttgcg attgcacgtg cgcttgtgaa taaccccgat
      361 tgtgtactgg cagatgagcc cactggtaat ttagatgatg aaaatgccca tcacgtattt
      421 gaagtgatgc aagcgcttaa tcgaacgcac aagaccagct tgattattgt aacgcacgat
      481 tccgaattgg cggcaaaaat ggatcggtcg tatgtgttac atgggggtca tttgtcttag
      541 ggcgattact gcggtccgtc cctacagatg attcgttaaa ttaagaaaaa aaatgacaaa
      601 cacattcatc actagcgtta tcagcttctt tttaggtatt attagccttc attttttccg
      661 tgaattaccc agcgtcgcgc ttgtaatgat aatcattgtt gctgtaggca gcatttcatg
      721 gattccacag tgtcaaaaat tcaaacccca gttattttta gcttatttga tcggcttttt
      781 atgggtgtta ttgaatgcca aatctgttgt cgatcaccgg atcccagacc accttgaagc
      841 aaaacccatt agcgtcgtcg gcattatcga tagtattcca gagcatcggg ataatacaac
      901 acgatttgat tttaatattc aacacacatt accagagtca ctttggccta atccaggacg
      961 cgtaaagcta cgatttagaa atcctacaat gccgttacac gttggcgatc aattgcaact
     1021 aacggttaaa ttaaaaagac cacagagtta tgcgaatccg ggaagctttg atacagagcg
     1081 acatttattt caacatcaca tggttgccga gggcacagtt gttaataaat cgagccgaca
     1141 cgaaaatctt acccctacaa aaataaacga ttctgtctta agccatccca tcgatcgcct
     1201 cagaatgtgg ctgttagaaa agataaatcg tgcattaaaa aacagtgaat attcaggcgt
     1261 tatcgatgcc ttagtggtcg gcctacagtc tggtattacg agtgaacagt ggaaagtact
     1321 aaaggatacg ggcacggtac atttggttgc tattgccggg ttgcatattg cctttgtaac
     1381 gggttttgtt atgttaatta ttttgcagat ttggcagaga ttgccgagga agctatatga
     1441 aataattttt ccaaaatttt tgaaaatacc cgtctctttg ttaaccgcag tgggtggatt
     1501 atctatcgca tttttctatg cggcattagc tggattttct attccgacac aacgagcagt
     1561 ggttgtcatt agcactttta tggtgagtat tttaagcaag cgccatgctt ctgtctggca
     1621 aaactatttt ttagcgctgt ttattgttgt gttattagat cccttatcca cattagcacc
     1681 cggtttttgg ctctcgtttg gggctgtggg gcttatttta tatggcatga aaggccgatt
     1741 acaccccaaa ggactctggt ggaagtgggg gcgtattcaa tgggtgcttt tcttagggct
     1801 ctcgcctatc atgctcgcta tttttggtat gacatcgtta attgcacccc tggcaaattt
     1861 tattgcgatt ccctgggttg ggtttttggt tttgcctttt tgtttactgg gtaccttatg
     1921 cgttactttt agcactggtt taggcacatt ttttttagat atcgcaaacg gatctttatc
     1981 attacttttt ccggtattgc agtggcttag tgaatttaga ggcgccactt gggttaacgc
     2041 aacccaaccc ttatggtcga tattgatagg atttatgggt gtcttattga ttttattacc
     2101 caaaggattt cctggaaaaa atatcggatt tattttttta ttgccaatat ttttaataaa
     2161 acctgtgatg gttgatctga ataccgcacg cgtaacggta ttagatgtgg gacagggact
     2221 ggcattgatc gtagaaactc aaggtcgtgt gttggtattt gatacaggcc ctaaattaaa
     2281 tccgggtttt gatacgggcg atcgagtcgt tgtgcctttt ttagcaacac gtgggcgaaa
     2341 taatatcgat gcattagtga ttagccatgg cgataacgat catgcaggtg gcgcacgttc
     2401 tattttaaaa gcgatgccgg ttaaaacaat tataaccagc gaacccaaat tatttccgga
     2461 atacaataca atggcttgtt ttgcagggca acagtgggca tgggatgggg tgcactttga
     2521 aatgttgcat ccaacaacgg taatgactaa aaaacgaaat gatcattctt gcgttttaaa
     2581 agtaacagcg ggtacgcaaa gtatcttgtt aactgcagat attgaggcaa aaagcgaaca
     2641 cagtattgtc gaaaatatgc ctgaaaaatt gccggctacc gttatgctgg taccacatca
     2701 tggtagtcga acctcttcaa cacttgaatt tattcgcgcg attaatcctc aaattgcaat
     2761 cattccggtg gggtatttaa atcaatatgg acatccaaaa ccggatattg ttgagcgcta
     2821 caaaaaagaa aatatcatag tgctggatac cgtaaaacag ggagcaattt catttattct
     2881 gaacaaaaaa aaccataata ataacgtgga tattcatgcg tatcggcaag aaaatcagcg
     2941 ttattggcat tcgggcggat tttaacccag tttacaattt aataccgatc tactgtaaac
     3001 tatgcttatt atagaaatac aggaagaatc atgtctgtta attatctaac ttctgctcag
     3061 gtttatcgcc gtttactgaa ggctgctctg ccgtattggt gggcctttgt tttaggtgtt
     3121 ttgggtaacg ccctgatcgc agcatcagat ggttggataa cttatggctt aaaaccactc
     3181 attgaaaaag gttttattgc cagggatccg atctttattc gttggattcc cattatcgtg
     3241 attacgtttt ttattgcgcg tggcgcttct tattttgtcg caagctactt tatgacctgg
     3301 gttgggcgca gtgttgtgcg tgattttcgt cggaaaatgg tcacacattt aatgtgttta
     3361 ccggcatctt tctttgatca aaaaaccagt ggcgaattgc tttctaaaat taattatgac
     3421 gctgatcaag tcgcagaagc catcagcgaa gccattacca gcgctattcg aggtgtattt
     3481 acaacggt
//
LOCUS       DAORVT010000112         3482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_22546_length_3482_cov_3.657718, whole genome shotgun sequence.
ACCESSION   DAORVT010000112 DAORVT010000000
VERSION     DAORVT010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22546_length_3482_cov_3.657718"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..1136)
                     /locus_tag="PK389_03000"
     CDS             complement(150..1136)
                     /locus_tag="PK389_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HQY22711.1"
                     /translation="MASFRQRKNTWRVEISVNGIRESATFDTKAQCKAWASKRETELR
                     EQAHGKLPNHLFTEAIDRYILEVSPKKKSHKKEVDRLNFFRRENAKLCKKQLSQVTTD
                     DLVKWRDSRLLVVQGATVRREANILASLFTIARREWKWIKDSPLSDLKLPPPAQHRDR
                     RITQDEIDRLILASNWGEHEPTNSTQQVIIAFLFALETAMRAGEIVQLSWDRVFLNER
                     YVVLKETKNGTKRNVPLSKRAVELLGYLKPLDKKHVFTCNHQSFDTLWRKLRNRCEIV
                     DLHFHDSRHEACTRLARKLDVLDLARMIGHKDLKSLMIYYNATASEIATRLD"
     gene            complement(1412..1936)
                     /locus_tag="PK389_03005"
     CDS             complement(1412..1936)
                     /locus_tag="PK389_03005"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016030408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HQY22712.1"
                     /translation="MTIVITAGHSNTDSGAVNGSDRECDIAQDMRNIVAYYLRAKGIN
                     IKTDGEGKGNATLTQAVKLIKGSAVAVEFHCNAASSKAARGVEALAQTKDKVISQRLC
                     SAVSSVMGSVLRGDKGWKPENSGQHSRLAYVSNGGIILELFFISNDEELKIWKEKKWL
                     VGKAVAEVLANYVT"
     gene            complement(1939..2166)
                     /locus_tag="PK389_03010"
     CDS             complement(1939..2166)
                     /locus_tag="PK389_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22713.1"
                     /translation="MSKLTFDVGREIYQTAVNAMDAAEKERESGADKKAWVLAFVKSF
                     VNDIGDNWERWAKVIITFIDFAKSVFNSKRG"
     gene            complement(2159..2443)
                     /locus_tag="PK389_03015"
     CDS             complement(2159..2443)
                     /locus_tag="PK389_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22714.1"
                     /translation="MDFLSNLATLVFILIFLLLVVHPRIKLAPYIDVFLWAGIMSGCA
                     SLINGAGVYTINGILFECIVAVLAIIFCWRLFKREVLGGQHMLKRRKTDV"
     gene            complement(2427..2750)
                     /locus_tag="PK389_03020"
     CDS             complement(2427..2750)
                     /locus_tag="PK389_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22715.1"
                     /translation="MAEIIAAFIAYLKSHIGVLIMGFLGASLSVLLSKEKYIDRLVGA
                     LAGFILCIAFAEHASQILANGKFPEVFGFLLGAMGKGTAETLLQFMRSKFVGRITGDY
                     DGFSK"
     gene            complement(2754..3029)
                     /locus_tag="PK389_03025"
     CDS             complement(2754..3029)
                     /locus_tag="PK389_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22716.1"
                     /translation="MTNIAQVAVGVINLIEDGQFIYSIAANAMDAVEALQDSELTGAD
                     KKKWVMAFVKSAVFNIIENWDAYAALISDFIDKIKTAYNAVRGLFGG"
     gene            complement(3095..3325)
                     /locus_tag="PK389_03030"
     CDS             complement(3095..3325)
                     /locus_tag="PK389_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004661619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22717.1"
                     /translation="MSNALSRLLDLLPKTPEFIATVQSADHPNYKVLVVDGTGLVLCT
                     SSTVFSMGDRVYISGNEIKRSAPTGIVYQIEV"
BASE COUNT         1009 a          822 c          616 g         1035 t
ORIGIN      
        1 tcagtatcct gtttaccatc attcccaaag ccgcgctatg tgattcggtg cggtcagggc
       61 agacgttgga atttggcaga agtatctaat tggcttaata accaatccga cgatgaatca
      121 acaaaaggac gaccacgcaa acaacgaggc taatcaagcc tcgttgcaat ttcacttgcc
      181 gtagcattgt agtaaatcat cagacttttt aaatccttgt gcccgatcat tcttgccaaa
      241 tcgagtacat ctaactttct tgcgagtcgc gtacatgctt catgtcgtga atcatgaaaa
      301 tgcaaatcta caatttcaca tctatttctc agtttgcgcc aaagtgtatc gaaactttga
      361 tggttacaag tgaatacgtg ttttttgtcc agtggtttta aataacccaa taattccaca
      421 gcgcgtttag aaagcggcac attccgctta gtgccatttt ttgtctcttt tagaacaaca
      481 taacgctcat tcaaaaatac cctatcccaa gataattgaa caatttcccc tgccctcatg
      541 gctgtttcga gcgcaaataa aaatgcgata atcacttgct gggtagagtt ggtaggttca
      601 tgttcacccc aatttgacgc aagaattaat ctatcaatct cgtcttgcgt aatacgtcta
      661 tctctatgct gtgctggcgg cggtaatttt aggtcggaca atggtgagtc tttaatccat
      721 ttccactctc tacgagctat agtaaaaagc gaagcgagta tatttgcttc acgccttact
      781 gttgcaccct gcactaccaa taaacgagaa tcacgccatt tcacaagatc atccgttgtt
      841 acttgtgata attgtttttt gcagagtttc gcgttttcgc gtctaaaaaa gttgagacga
      901 tcaacttctt tcttatgact tttcttttta gggctgactt caagaatgta tcggtcgatt
      961 gcttctgtaa acaaatgatt gggtaacttt ccgtgagcct gctctctaag ttccgtctct
     1021 cgttttgatg cccaagcttt acattgagct ttagtgtcaa acgtggcact ctctcgaata
     1081 ccatttacac tgatttcaac tctccacgta ttcttgcgtt gtctaaatga agccactggc
     1141 gtaatcttgg cgtaattgaa ttaagcgaaa taatagggaa taataagaaa taatagaagt
     1201 acatagtaat tggtttattg ttaatcccat gtttgtactc agaataatga caaataatag
     1261 aatttaatag gaaaacacac aaattagtaa agtgcccgcc gagcgcacca taacacctta
     1321 taaaatcaat aacataaata catattggcg tagatttagc gtaacacatc aaaaaaggcg
     1381 catgatttgc gccattttct tgagtgataa gctaagtaac gtaatttgct agcacttctg
     1441 ctacagcttt gccgacaagc cattttttct ctttccaaat ctttaattct tcatcattgg
     1501 atataaagaa cagttcaaga ataatgccgc cattgctgac ataggctaag cgcgaatgct
     1561 gaccagaatt ttcaggcttc cagcctttat cgccacgcaa tacactaccc atgaccgatg
     1621 aaactgcact gcataaccgt tgactgatta ctttgtcttt ggtttgcgca agtgcttcaa
     1681 caccccgagc tgctttactt gatgctgcat tacaatgaaa ttccactgcg acggctgacc
     1741 ctttaatgag tttgaccgct tgagtgagcg tagcattgcc tttaccctca ccatcagttt
     1801 taatattgat gccttttgct cgcaaatagt acgcaacaat attacgcata tcttgcgcga
     1861 tgtcacattc acgatctgaa ccattgactg ctcctgaatc tgtattgctg tgccctgctg
     1921 tgattacgat agtcatcatt accctctttt gctattgaat accgacttag caaaatcaat
     1981 gaacgtgata atgactttcg cccaacgctc ccaattatcg ccaatgtcat tcacaaacga
     2041 ctttacgaac gcaagtaccc atgctttttt atcggcaccc gattcgcgtt ctttttccgc
     2101 tgcgtccatc gcattgactg cggtttgata tatttcacgt cctacgtcaa atgttagttt
     2161 agacatcggt ttttctccgc ttcaacatgt gctgaccgcc aagcacttca cgcttaaaca
     2221 gccgccaaca aaatataatc gccaacaccg ccacaatgca ctcaaacaag atgccgttga
     2281 tcgtatatac gccagcgccg ttgatgagcg atgcgcaccc tgacataatc ccagcccaca
     2341 aaaacacatc aatataaggc gcaagcttaa ttcttggatg tacaaccaaa agcagaaaaa
     2401 taagaataaa aacaagcgtt gctagattac ttagaaaatc catcataatc tcctgttata
     2461 cgcccaacaa attttgagcg cataaactgc aataaggttt cagccgtacc ttttcccatt
     2521 gcgccaagta gaaagccgaa cacttcaggg aacttgccgt tggctagaat ctgtgaggca
     2581 tgttcagcga atgcaataca caaaataaag cctgccaatg cgcccacaag tcggtctatg
     2641 tatttttctt ttgacagcag cacactaaga cttgctccca aaaaacccat aatcaaaaca
     2701 ccaatatgac ttttaagata tgcaataaac gccgctataa tttcggccat ttctcaacct
     2761 ccaaacaaac cgcggacagc gttataagca gtcttaatct tgtcaataaa gtcagaaatt
     2821 aaggctgcgt aagcatccca attttcaatg atgttaaata ctgcggactt tacgaaagcc
     2881 atcacccatt tctttttgtc tgcgccagtt aattcactat cttgcagagc ttctactgca
     2941 tccatagcgt ttgctgctat tgagtagatg aactgcccat cttcaattaa gttgattacg
     3001 cctactgcga cttgtgcgat gttggtcatt ggctacgact cctaaatttt ggtattaaaa
     3061 aaccgccaaa aaggcggctt catttgtttt aaagttaaac ttcgatttga tacacaatac
     3121 ccgtgggcgc gcttcgtttt atttcatttc cactgatata cactcgatct cccatgctaa
     3181 aaactgttga actggtacac agcacaagcc ctgtcccgtc cacaaccaaa actttatagt
     3241 ttggatgatc tgcactctga acagtggcta taaactcagg cgttttgggc aataaatcta
     3301 aaagtctcga taatgcatta ctcatgattc actctctcta ttgatgcagt ttgagtgact
     3361 ttttcataac taaatgatcc gcttacacca tcagcaatgc cccaccagtc gccgttaaaa
     3421 gcaattaatt ctgcgggtac acattgaccg acttcttgag taatcggcat caataagcta
     3481 tg
//
LOCUS       DAORVT010000113         3477 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_22606_length_3477_cov_3.525716, whole genome shotgun sequence.
ACCESSION   DAORVT010000113 DAORVT010000000
VERSION     DAORVT010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22606_length_3477_cov_3.525716"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..1534)
                     /gene="bioA"
                     /locus_tag="PK389_03035"
     CDS             complement(206..1534)
                     /gene="bioA"
                     /locus_tag="PK389_03035"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006035075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HQY22718.1"
                     /translation="MTLIKRDLSHIWHPCSQMKDCEKWPLLEIRRAYGSYLELADGTR
                     LIDAISSWWCKSLGHGQPRLKAALLRQVDQYEHVILANTCQETLVELSEKLALLCPQL
                     NKIFYASEGASAVEIAVKMSLHSRQILGKQDQKKNRIMALQNAYHGETLLTLSLSDVG
                     IYKKFYQNWLMPVIFLQDIPYVCSEADPLWQDCGPAWINIENQLNEHIDDLSAIIVEP
                     IVQGAGGMLIYSQDFLKKLRAWTLKQDIHLICDEIMTGLGRTGFLLACEHAEIVPDFI
                     CLGKGLTSGWLPMSAVLTTKDIYQLFYDDYETGKSFLHSHTFSGNALAAAVALETLKM
                     MEEEKVCEKVREKQALLNQLMLDVARKTQRLTRVRCIGAIVAADLILEDHQKHQRVGD
                     EVFQNAIKLGAFLRPLGNTLYWLPPLNIENEILHELKDISVQAINKTFRI"
     gene            1653..2609
                     /gene="bioB"
                     /locus_tag="PK389_03040"
     CDS             1653..2609
                     /gene="bioB"
                     /locus_tag="PK389_03040"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015781148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HQY22719.1"
                     /translation="MNPTISLRNDWTLQEIDHLFSLPLMDLLFQAQSVHRDTFPKNTV
                     QLSTLISLKTGACSEDCAYCPQSGRYQTSIQKHRLLGTETVKVTALAAKANGATRFCM
                     GAAWRSPPQHVFEQLLLILKVVKDIGLETCMTLGMLTANQAQQLSNGGLDYYNHNLDT
                     SPEYYTNIITTHTYVDRLETIEHVRTAGIKVCCGGIIGLGESKEDRVSFLQQLANLPE
                     HPKSVPINKLIPIKGTPLGKTPTIDSFEFVRVIATARILMPKSVIRLSAGREDMNDEF
                     QALCFLAGVNSIFYGEKLLTAKNPALEQDRNFLKRLGIQAST"
     gene            2613..>3477
                     /locus_tag="PK389_03045"
     CDS             2613..>3477
                     /locus_tag="PK389_03045"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HQY22720.1"
                     /translation="MTKLIQSLVDKLFQRQELGLYRSRHIITERNRKILSFMNNDYLG
                     LANHPEVIHTFQKNAAYYGLGSSASQLLGGYYIEHQRLEEKLAEFLGLERALIFSSGY
                     MANLSVLTTLINNQDCAFEDRLNHASLIDGVRYSNAQVKRYRRNNLLMLEKRIAQTYA
                     KNKWIITEGVFGMDGDVAPLPQLFHLAQSYDCQLLIDDAHGIGVLGKSGRGSIEHYQL
                     PIKNASIVVGTFGKAFGTMGAFVAGHHVMIEALMQFGRGFIYTTALPPAIASATCTSL
                     RLIQTENWRREN"
BASE COUNT         1072 a          727 c          634 g         1044 t
ORIGIN      
        1 gatcgtgtta ggtaatttgg gtaatggtta tttacacaaa tttatggaca gactcgccgc
       61 ctacttggaa ctggcaattc gaaagagtat tccatttagt tcttttgata acactttgtg
      121 tgctgcagcg gagaaggcag gcatcgattt gctctaatac ttatgaagct aaaatgcagc
      181 tgcccgcaat gcagccaata agattttaaa ttctaaaggt tttatttatt gcttgtaccg
      241 aaatgtcttt taactcatgt aaaatctcat tttcaatatt cagtggcggt agccaataaa
      301 gagtgttacc caaaggccgt aaaaaagcgc ctaattttat agcattttga aacacctcat
      361 cgcccactct ttgatgcttc tgatgatctt ccaaaatgag atcggccgca acaattgcgc
      421 caatacaccg cactcgagtt agtcgctgtg tctttcgagc aacatcaagc atcaattgat
      481 ttaacagcgc ttgtttttct cgcacttttt cacaaacttt ttcttcttcc atcattttta
      541 aagtttctaa agcaaccgct gccgctaacg cattgccact aaaagtatgc gaatgtaaaa
      601 acgactttcc tgtctcataa tcatcataaa ataattgata aatatctttt gtcgttaaca
      661 ccgcactcat gggcaaccaa cccgaagtta accctttccc aagacaaata aaatcgggaa
      721 ctatttccgc atgctcacaa gccaaaagaa accctgttcg acccagccct gtcataattt
      781 catcgcagat taaatgaata tcttgtttta atgtccaagc acgtagcttt tttaaaaaat
      841 cttggctata aatcagcata ccacctgcac cttgaacaat gggttcaaca ataattgcgc
      901 ttaagtcatc tatatgttcg ttaagttgat tttcaatatt gatccatgca ggtccacagt
      961 cttgccacag cggatctgct tcagaacaca catacggaat atcttgtaag aagatcacag
     1021 gcatgagcca gttttgatag aattttttat aaataccaac atcactaagc gataaagtca
     1081 acaaggtttc gccatgatat gcgttttgca aagccattat tcgatttttt ttttgatctt
     1141 gttttcctag gatttgtcta ctgtgtaaac tcatcttaac ggctatttcg actgcagaag
     1201 cgccttctga ggcataaaaa atcttattta gttgcggaca caataaagca agcttttcgg
     1261 ataattcgac taaagtttcc tgacacgtat ttgctaatat cacatgctca tactgatcga
     1321 cttgtcgcaa taatgctgct tttaacctcg gttgcccatg ccctagagat ttacaccacc
     1381 agctggaaat agcgtctatc aaccttgtgc catcggctaa ttctagatac gaaccataag
     1441 cacgccggat ctcaagcaat ggccattttt cgcaatcctt catttgggag cacggatgcc
     1501 aaatgtgcga tagatctcgt ttaattaatg tcatttgaat aaccaccttg tttattgtcg
     1561 acaatttaat cttaaataag ttgacaatat attgaaagaa catttatctt tagccgtctc
     1621 tactgttgaa ctatttattt tcaggaaaag ttatgaaccc aacaatatct ttacgcaatg
     1681 attggacgct tcaagaaatc gatcatttat tttcattgcc gctcatggat ttactttttc
     1741 aagcgcaatc tgttcaccga gacacctttc ctaaaaatac cgtacaactc agtacattaa
     1801 ttagccttaa aacgggtgcc tgctctgaag attgcgctta ttgtccgcaa agtggacgtt
     1861 accaaacaag catacaaaaa catcgactat taggtactga aacagtcaaa gtcactgcgc
     1921 tcgcagcaaa agcaaatggt gcaacacggt tttgtatggg tgccgcgtgg cgaagcccac
     1981 cacaacatgt gtttgaacaa ttattattaa tattaaaagt cgtaaaagat ataggtttag
     2041 aaacttgtat gactcttggt atgctaaccg ctaatcaggc acaacaattg tcgaatggcg
     2101 gtttagatta ttacaatcat aatttagata cctcccctga atattataca aacatcatta
     2161 caacgcatac ctacgtagat cgcttagaaa caatagaaca tgtgagaaca gctggtatta
     2221 aagtgtgctg tggcggtatt ataggattag gtgaatccaa agaagatcgc gtaagctttt
     2281 tgcagcaact cgccaatctc ccagaacatc caaaaagtgt tcccattaat aagctaattc
     2341 ccattaaggg aacgccgtta ggtaaaacac ctaccattga ttcctttgaa tttgttcgag
     2401 ttatcgctac cgcacgtatt ttaatgccta aaagcgtcat tcggctttcc gcaggcagag
     2461 aagatatgaa cgatgaattt caagcgcttt gttttttagc aggtgtaaat tccatttttt
     2521 acggagaaaa attattgacc gcaaaaaatc ccgcattaga acaagatcgg aattttctaa
     2581 aaagattagg tattcaagca tcaacttaaa aaatgacaaa attaatacaa tctttggttg
     2641 acaaactgtt ccaacgacag gagttaggtt tataccgttc tcgccacatc attaccgaac
     2701 gcaatcgaaa aattttatct tttatgaata atgactattt aggattggcg aaccatccag
     2761 aggtgattca cactttccaa aaaaatgcag cctattatgg tcttggcagc agcgcttctc
     2821 aactactcgg tggatattat atcgaacacc aaagactcga ggaaaaactc gccgagtttt
     2881 taggacttga acgtgcattg atcttttcaa gtggatacat ggctaatctt tcggtgttga
     2941 ccacattgat taacaatcag gattgtgcat ttgaagatcg cctaaatcac gcttctctta
     3001 tcgatggcgt tcgctatagc aacgctcagg ttaaacgcta tagacgcaat aatttattga
     3061 tgcttgaaaa acgaattgct caaacatacg caaaaaacaa atggattatt actgaaggcg
     3121 ttttcggtat ggacggtgat gttgcacctc taccccagct ctttcattta gcacaaagtt
     3181 atgattgcca actattgata gacgatgcac acggcattgg agtattagga aagagtggtc
     3241 gcggcagtat agaacattat caactgccca taaaaaatgc ttctattgtc gtgggtactt
     3301 ttggtaaggc attcggaacg atgggtgcgt ttgttgcagg acatcatgtc atgatagaag
     3361 cattaatgca atttgggcgc ggttttattt atacaaccgc attaccgcct gcaattgcat
     3421 ctgccacctg tacgagttta cgattaatac aaaccgaaaa ttggcgccga gaaaatc
//
LOCUS       DAORVT010000114         3471 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_22671_length_3471_cov_3.133782, whole genome shotgun sequence.
ACCESSION   DAORVT010000114 DAORVT010000000
VERSION     DAORVT010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22671_length_3471_cov_3.133782"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1809)
                     /gene="htpG"
                     /locus_tag="PK389_03050"
     CDS             complement(<1..1809)
                     /gene="htpG"
                     /locus_tag="PK389_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HQY22721.1"
                     /translation="MSESTVKKETFGFQAEVKQLLHLMIHSLYSNKEIFLRELISNAS
                     DAADKLRFESLRDPMLLVDEPDLKVWVLLDKEAKTITIRDNGIGMTREEVIQNLGTIA
                     KSGTREFLSTLTGDRAKDAQLIGQFGVGFYSSFIVADKVVVTTRKAGAPVTEGVQWSS
                     AGQGDYELETVSKDTRGTEVVLHLKADEDEFLNDWRLKHIIMKYSDHLTLPVVVKNEK
                     NEDEVVNRATALWTLSKNEITDEQYKELYKHVSHDFQDPLVWTHNKVEGKLEYVTLLY
                     IPEHAPFDLWHVGKTRGLKLYVRRVFIMDDAEQFLPHYLRFVRGIIDSNDLPLNISRE
                     ILQSNRIIDTIKTAVIKRVLGMLEQLATDDKPKYQIFWNAFGQVLKEGPAEDFANKEQ
                     IAKLLRFASTHLDSAEQTVSLEDYIARMPAGQTKIFYVTADTFNAASHSPHLEIFRKK
                     GIEVLLLTDRVDEWLMTHLTEFQGKPVQAVTRGGLDLGDLADKTAEEHYKQAETDFEE
                     TVKKVKELLESSVKDVRLSQRLTDSPACIVLDEQDMSGQMERMLRASGQQVPTQKPIL
                     ELNPDHRMIKHLKDEQDSARFEDWSRILLDQAILAEG"
     gene            complement(1827..2423)
                     /gene="recR"
                     /locus_tag="PK389_03055"
     CDS             complement(1827..2423)
                     /gene="recR"
                     /locus_tag="PK389_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020412025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HQY22722.1"
                     /translation="MSFSPLINQLINALRCLPGVGPKSAQRMVFHLLERDRNGGQLLA
                     RILEEAIAKVGRCQQCRTLCEDPLCRFCDNPLRDRSIVCVVETPADVLAVEHTAGYRG
                     VYFVLMGHLSPLDGIGPEDLGMDLFRKRLQEGEIKEVIVATNPTVEGEATAHYLAELV
                     KKHQIEVTRIAHGVPFGGELEYIDGGTIARAMLARAPL"
     gene            complement(2437..3420)
                     /locus_tag="PK389_03060"
     CDS             complement(2437..3420)
                     /locus_tag="PK389_03060"
                     /inference="COORDINATES: protein motif:HMM:NF023591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau C-terminal
                     domain-containing protein"
                     /protein_id="HQY22723.1"
                     /translation="MQELARAADGSLRDAMSLLDQALNYCEGTVEARSVRLMLGLSEK
                     ARLLALLGSLANGNAKSILNEINALQDSAPDFSGLLTEFLELIHQVAIAQSVPEALDE
                     NRRDREAILAFAKSILPEEIQLYYQIGLQGQRDLPYAPNPKMGFEMILLRMLAFQPVK
                     VSNFVAKSKSQVEASVHVEAKEVTSEPAVMQQKPEPDRVLERQDWSSIVKSLNLKGLE
                     KVLAINCTVAAWEDDLISLNLDESQKPLFENKRYEDRLREALSQHLGQSIRLKITCGA
                     AVGETPALQTRRLLTAVQEAASNQAEADPEIQKLIKAFDAKIEHIALKEEQ"
BASE COUNT          958 a          844 c          590 g         1079 t
ORIGIN      
        1 accctctgcc aaaatggctt gatccaataa gatccgcgac caatcttcga atcgcgcaga
       61 atcttgttca tctttaagat gcttaatcat tctgtgatcg ggatttaatt ctaaaatggg
      121 tttttgtgtt gggacttgtt gtcctgaagc acgtaacatc cgctccattt gaccactcat
      181 atcttgctca tctaatacaa tacaagccgg cgaatcggtt aaacgctgac ttaatcgcac
      241 atcttttaca gaagactcta gcaactcttt cacttttttg accgtctctt caaaatccgt
      301 ttccgcttgt ttatagtgtt cttctgctgt tttatctgcc aaatcaccca gatctaaacc
      361 accgcgcgta accgcttgta caggcttacc ttgaaattct gtgagatgtg tcattagcca
      421 ttcgtctaca cgatcagtta ataataatac ctcgatgcct tttttgcgga aaatctctaa
      481 atgtgggcta tggcttgccg cattaaatgt atctgctgtt acataaaaga tttttgtttg
      541 acccgctggc attctcgcaa tgtagtcttc taatgaaacg gtttgctcgg cactatccaa
      601 atgcgtactg gcaaatctca ataattttgc aatttgttct ttattcgcaa aatcttctgc
      661 tgggccttct tttaaaactt gaccaaatgc attccaaaaa atttgatatt ttggtttatc
      721 atctgttgcc aactgttcta acatacccaa cacacgtttt ataacggctg ttttaattgt
      781 atcaattatc cgattgcttt gcagaatttc tcttgaaata tttaatggca aatcattgga
      841 atcgataata cctcgaacaa atcttaaata atgaggcaaa aattgctctg catcatccat
      901 aataaagaca cgtcgcacat acaattttag gccgcgcgtt ttacccacat gccacaaatc
      961 aaatggcgca tgttctggga tatacaacaa ggtaacatat tcaagcttcc cttcgacctt
     1021 attatgtgtc caaactaaag gatcttgaaa atcatgtgaa acatgtttat ataattcttt
     1081 gtattgctca tcggtaattt cattttttga caaagtccat aatgctgttg cacgattaac
     1141 aacttcatct tcattttttt cgtttttaac aacaacaggc aaggtcaaat gatctgaata
     1201 tttcataata atatgtttta aacgccaatc gttcaaaaac tcgtcttcat cggccttcaa
     1261 atgtaataca acttcagtac ctcttgtgtc tttagataca gtttccagtt catagtctcc
     1321 ctgacctgca gagctccact gaacgccttc tgtgacaggt gcaccagcct tacgggttgt
     1381 aaccaccact ttatcggcta caataaacga tgaataaaac ccaacgccaa actgaccaat
     1441 caattgtgcg tcctttgccc tgtcaccggt taatgtactt aaaaattctc gtgtaccgga
     1501 ttttgcaata gtccctaaat tttggatcac ttcctctcgt gtcatgccaa tgccattgtc
     1561 tcgaatggta atggtttttg cttctttatc gagtaaaacc catactttta aatccggttc
     1621 atccactaat aacatcgggt cacgcaaaga ttcaaatcgc agtttatctg ctgcatccga
     1681 tgcattagaa attaactccc gtaaaaagat ttctttattg ctatataggg aatgaatcat
     1741 gagatgcaat agctgtttga cctcggcttg aaaaccaaaa gtttcttttt taacagttga
     1801 ctctgacata tttatctcct cgtagtttag agcggtgctc gcgccaacat tgctcgcgct
     1861 atcgtaccgc catcgatata ttctaactcg cccccaaaag ggaccccatg tgcaattcga
     1921 gtaacctcaa tctggtgttt tttaaccaat tctgccaaat agtgtgcggt ggcttcgcct
     1981 tctacggtag gatttgttgc aacaatcact tcttttattt caccctcttg caagcgtttc
     2041 ctaaacaaat ccatacctaa atcttcagga ccaatgccat ctaaaggcga caaatgaccc
     2101 attaacacaa aataaacgcc cctataccca gcagtgtgtt ccactgccaa tacatcagcg
     2161 ggtgtttcga ccacacagac tatcgaccga tcgcgcagcg gattatcgca aaaccgacac
     2221 aaaggatcct cacataaggt tcgacattgc tgacaacgcc cgaccttagc aattgcttct
     2281 tccaatattc gcgctaaaag ttgcccacca ttacggtcac gctccagcaa atgaaaaacc
     2341 attcgctgtg ccgatttcgg cccaacaccc ggcaaacacc gtagtgcatt gattaattga
     2401 ttaatgagcg ggctaaaact cacgtctata tcctctctat tgctcttctt ttaaagcaat
     2461 atgctcaatt tttgcatcaa aggctttaat cagcttttga atttccggat ctgcttctgc
     2521 ttgattacta gctgcttcct ggactgcagt caacaaacgt ctcgtttgaa gtgccggtgt
     2581 ttctccgaca gctgccccac acgttatctt caagcgaatc gattgtccta aatgctgaga
     2641 caacgcttct cgtagtctgt cctcataacg cttattctcg aacaaaggct tttgtgattc
     2701 atctagattg agcgatatta aatcatcttc ccatgcagcg acagtgcaat taatcgccaa
     2761 taccttttcc aaacctttca agttaagcga cttaacaata ctgctccaat cttgtcgttc
     2821 taagacccta tcgggttctg gtttttgctg catcactgct ggttcagaag tcacctcttt
     2881 cgcttcaaca tgaacagatg cttcaacttg tgatttagat ttagccacaa aattggatac
     2941 ttttaccggc tgaaaagcta acattcgaag cagtatcatt tcaaaaccca ttttgggatt
     3001 aggcgcataa ggcaagtcac gttgcccctg taaaccaatc tggtaatata attggatctc
     3061 ttccggtaat atactttttg cgaatgccaa tatggcttca cgatcacgcc tattttcatc
     3121 caatgcttct ggcacacttt gtgcgattgc aacttggtgg atcagttcta aaaattcggt
     3181 taacaagccc gaaaaatctg gcgcgctgtc ttgaagcgca ttaatttcat tcagaatact
     3241 ttttgcatta ccattggcta aactacccaa tagcgccaac aaacgggctt tttcagataa
     3301 ccccaacatt aaacgcacgg aacgcgcctc gacggtgccc tcgcaataat ttaaggcttg
     3361 atctaataaa ctcatcgcat ctcttaaact accatctgca gcacgcgcca attcttgcaa
     3421 cgcaccgtcg aggcgcgttc cgtgcgttta atgttggggt tatctgaaaa a
//
LOCUS       DAORVT010000115         3450 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_22922_length_3450_cov_3.735788, whole genome shotgun sequence.
ACCESSION   DAORVT010000115 DAORVT010000000
VERSION     DAORVT010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22922_length_3450_cov_3.735788"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="PK389_03065"
     CDS             <1..820
                     /locus_tag="PK389_03065"
                     /inference="COORDINATES: protein motif:HMM:NF024739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HQY22724.1"
                     /translation="YDIIMPSSYFISRMRRHALIQKIDHSKLEHYNNINPIFLSKEHD
                     PKNEYSIPYLWNGTGIAYNTRYHAQKSVNTWQDLWLPRYRDQLLVLDDTRECFSMALI
                     ALGYSVNDQDPEHIKAAFEKLKALMPNIKLFNMDAQRSIYLDEDITIGMGLNGDIFLA
                     KAQNPYLQFIYPKEGFVITLDSIAIPVGAKHVANAHRFIDFVLRPEIAKQITLESGFS
                     TPNLAAKELLPLQIRTDPILYPDVETLKKGQFHADVGEATPLYERYFERLKLEE"
     gene            1061..2455
                     /locus_tag="PK389_03070"
     CDS             1061..2455
                     /locus_tag="PK389_03070"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HQY22725.1"
                     /translation="MKRKYSGITKTLVGGLVVLGMAASSNAFAVQARENKNKVTENAN
                     AENVTEGFDLGKGWLLNSTGGLSYTNENCSDYWFKLSGLLRFDEALFMGSYRDKGNNY
                     PSGGNIRTADLYIEGGLAQDWTYNISLALNSSNRNMFGDTWISYAGFAENNQVYFGRV
                     PGNWFGLDNANSGTWGPFLERSLVANTFYPGDGIGFMTDFWSDSAGITFTAMKSDQNS
                     SNEVSNVRDRWKGTVRATVAPMHEEGDVWHFGVSGAYRELQNVVPTSASSVGALFQAF
                     PGVKIRNNSATSLTSMINVVSTLTGNGGLPIQANNIRMFNLEAARQIGPFMLETEYTK
                     AYVHRMGTQPFAGTTVQQGTLQFSGWNIQTRYLLTGEHHEYDVRDGQFGSVKPCSEHG
                     AIEAAARYDFVDLNDKNVRGGSQHDVTLGLNWFLNQNVRLSMNYIRASIHPANDKAHR
                     NLDIIGLRAQIRFK"
     gene            complement(2498..>3450)
                     /gene="xseA"
                     /locus_tag="PK389_03075"
     CDS             complement(2498..>3450)
                     /gene="xseA"
                     /locus_tag="PK389_03075"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HQY22726.1"
                     /translation="KVSLYPERGDYQLIVDRIEMAGDGLLKKAYEELVSKLTKEGLFE
                     ERWKKPLPLLPKQIGVITSPTGAAIRDILSVLKRRFPSIPVIIYPTKVQGSEAMLEIV
                     QAITLANQHQATNILILARGGGSLEDLWPFNEEKVARAIFDSQIPIVTGIGHEIDFTI
                     ADFVADRRAATPSAAAELVVPDQYAFLQQLSTLENRLINEITKYCQRLAQRLDGLIKQ
                     IRHPGQQIQNQLQTLSLLMIRLNNTMQQLLKTKDLALKNALRALETVSPLATLNRGYA
                     ILSLADRDHVVRSIQEVKHGDNVQIRLEDGILKAKVYDTN"
BASE COUNT          964 a          653 c          809 g         1024 t
ORIGIN      
        1 atacgatatt atcatgcctt caagttattt tattagtcgt atgcgaagac atgccttaat
       61 tcaaaaaatc gatcattcca aattagaaca ttataataat attaacccga tttttttaag
      121 caaagaacac gatcccaaaa atgaatatag tattccttat ttatggaacg gaacgggtat
      181 tgcatataat acgcggtatc atgcacagaa gagcgtcaat acctggcagg atttatggtt
      241 accacgctac agagatcagt tacttgtttt ggatgatacg cgagagtgtt tttcgatggc
      301 cttaattgcc ctgggttatt cagtgaatga tcaagatcct gagcatatta aagcggcatt
      361 tgaaaaacta aaggctttaa tgcccaatat taaactgttt aatatggatg ctcaacgatc
      421 tatctattta gatgaagata ttacaatagg aatgggatta aatggggaca tttttttagc
      481 gaaagcgcaa aatccatact tgcagtttat ttatccaaag gaaggctttg ttattacctt
      541 agacagtatc gcaattcctg tgggagctaa acatgtggca aatgcccatc gttttattga
      601 ttttgtgtta cgtccggaaa tcgcaaaaca gataacgtta gagagtggtt tttctacacc
      661 gaatttagcc gccaaagaac tgctacctct gcagattaga acagatccca ttttatatcc
      721 cgatgtggaa actttaaaga aagggcaatt tcatgcggac gttggtgagg caacgccgct
      781 ttatgaaagg tatttcgaac gattaaaatt agaagagtag ggcggactgc tgtgttcacc
      841 tccattttga tgggctagcg gccagtgccg agacttcatt taccgcataa gcacagaaga
      901 ccaataatga atttaataaa tgcttattgt tctagtgttg cgtattagcg atgctagtag
      961 tagcatgcgc aacacgagtt gtgctaaggt cttattttgc gatgcatcta aagatgcgct
     1021 atgtgtatgt gttaagccaa cgattaggag gcaggattaa atgaagcgga agtactctgg
     1081 gataacgaag acgttagtag gtgggttggt tgtattaggg atggctgcga gcagcaatgc
     1141 ttttgcagtg caggcgcgcg aaaataaaaa taaagtaaca gagaatgcga acgcagaaaa
     1201 tgtgactgaa ggttttgatt tagggaaagg atggcttctt aattctacgg gaggtctttc
     1261 ttataccaac gaaaattgtt cagattattg gtttaaatta agcggtttac ttcgatttga
     1321 cgaagcgctt tttatgggta gctatcgtga taaggggaat aattatccca gcggcggcaa
     1381 tatccgtaca gcagatttgt acattgaagg tggccttgct caggattgga catacaatat
     1441 atcgttggca ttaaattcta gcaatcgcaa catgtttggc gatacctgga tttcttacgc
     1501 aggattcgca gagaataacc aagtttattt tggacgcgta ccgggtaact ggtttggttt
     1561 agataacgct aatagcggta catggggtcc cttcctagaa agaagtttag tcgctaatac
     1621 gttttatcca ggcgacggta tcggttttat gaccgatttt tggtcagatt ctgcagggat
     1681 tacctttaca gcgatgaaat cggatcaaaa ttcatccaac gaggtttcga atgtgcgcga
     1741 tcgttggaaa ggaacagttc gtgcaacagt cgcgcctatg catgaagagg gcgatgtctg
     1801 gcattttggt gtttcgggag cgtatcgtga acttcaaaat gtagtgccta caagtgcgag
     1861 cagcgttggt gctctgtttc aggcattccc tggggttaaa attaggaaca atagcgccac
     1921 tagtctgacc agcatgatca atgtggttag cacgcttaca ggcaatggtg gtttgcccat
     1981 ccaagcgaat aatattcgca tgtttaacct cgaagcagcg cggcaaattg gtccctttat
     2041 gctagaaact gagtacacaa aggcttatgt gcatcgtatg ggtactcagc catttgcagg
     2101 tacgactgta cagcaaggta cgcttcaatt tagcggttgg aatatccaaa cccgatacct
     2161 gttaaccggc gagcatcatg agtatgatgt tagagatggt caatttggtt cggttaagcc
     2221 gtgctctgaa catggtgcaa ttgaagctgc agcgcgatac gactttgttg atctgaacga
     2281 caaaaacgta cgtggcggat cacagcatga tgtaacactt ggattaaact ggttcttgaa
     2341 tcagaacgtt cggttgtcta tgaattacat tcgtgcttcg attcatccag caaacgataa
     2401 agcacatcgt aatctagata ttatcgggtt acgcgctcag attcggttta agtaattggt
     2461 tgcgccacct tctctcgtca aacgggagaa ggtgcgctca atttgtatcg tagactttcg
     2521 ctttcagaat cccatcttct aatctaatct gcacgttatc tccgtgcttc acttcttgaa
     2581 tcgatcgcac gacatgatcg cggtctgcta aagaaaggat cgcatacccc ctattgagcg
     2641 ttgccagtgg acttaccgtt tctaaggcgc gtaaggcgtt tttaagcgca agatctttag
     2701 ttttaaggag ttgctgcatc gtgttgttta atcgaatcat cagcagagaa agtgtttgta
     2761 gctgattttg aatttgctga cccggatgtc gaatttgttt gattaagcca tccaaacgtt
     2821 gtgctaagcg ctgacaatat tttgtaattt cgtttatcag acgattttct aaggtgctta
     2881 actgttgtaa aaaagcatat tgatctggca ccactagttc tgcagcggca gaaggtgttg
     2941 ccgctcttcg atcggcaaca aaatcagcaa tggtaaaatc aatttcatga ccgattccag
     3001 taacaatggg aatttggctg tcaaaaatgg ctcttgcgac tttttcttcg ttaaaaggcc
     3061 aaagatcctc taagctgcca ccgcctctgg ccaaaattaa tatgttggtg gcttgatgtt
     3121 gattcgcaag cgtaattgcc tgaacaatct ctagcattgc ttctgagcct tgtacttttg
     3181 ttgggtaaat aataacgggg atacttggaa aacgtcgttt caatacagac aggatatctc
     3241 gaatggcagc acccgttgga gaggtaatca cgccaatctg tttagggagt agaggaagcg
     3301 gttttttcca gcgttcttca aaaagccctt ctttggttaa tttcgaaacc agttcttcat
     3361 acgctttttt gagtaagcca tcgcccgcca tttcgatgcg atcgacaatc agttggtaat
     3421 caccgcgttc ggggtatagg cttactttgg
//
LOCUS       DAORVT010000116         3433 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_23121_length_3433_cov_2.915038, whole genome shotgun sequence.
ACCESSION   DAORVT010000116 DAORVT010000000
VERSION     DAORVT010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23121_length_3433_cov_2.915038"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="PK389_03080"
     CDS             <1..895
                     /locus_tag="PK389_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQY22727.1"
                     /translation="RNFQYQTAISCLETIDKLHEKRIIHRDIKPENFMMHVRDAQGNV
                     IELKNIKPENFMRNGQNDVVVVVPIDFGLSRQLEVDQTVEIAPMVGTFGYIAPEVING
                     GKVSRETDIYALGKMLNKNFAIDIPEMYADASAERPSIKDVVTYLKIQAQKVQDKEPS
                     LPSFSVWKEAQSKTQLDPSAALAKAAAQVSKEVVAITQLSSTREMPTTTSPTHVLQFS
                     NAGTTGSISPFATKDGRPAPPPPGYTSDGKKAPPPPPRQENAGPVIHLPKEENSWKKA
                     WGPVIHVVEGDPNDNKHKPGG"
     gene            1115..1588
                     /gene="greA"
                     /locus_tag="PK389_03085"
     CDS             1115..1588
                     /gene="greA"
                     /locus_tag="PK389_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015702211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HQY22728.1"
                     /translation="MQQIPMTATGVEKLREELKHLKNVVRPRIIAAIAEARGHGDLKE
                     NAEYHAAKEQQAFTEGRIRDIEHKLSYVQVIDLTGMANNGKVVFSATVTLLNLDTDQT
                     MVYQIVGEEEADLKTGKISVTSPIARAVIGKYKGDTVEVTAPGGVVSYEVSEVIY"
     gene            1604..2299
                     /locus_tag="PK389_03090"
     CDS             1604..2299
                     /locus_tag="PK389_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014403264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HQY22729.1"
                     /translation="MTGKSRSKSTKSTKSKSSRRWLDRQHSDYYTQLAREKGYRSRSA
                     YKLLEIQEKDKIIFKGMRVVDLGAAPGGWSQVLVNHVGSSGKIVAIDILPIESLKGVD
                     TVYGDFQKTETLEELMLILDGKSVDLVISDLAPNLSGIAVADQARVMDLAEMALDFAE
                     RVLVKEGVFLIKVFQGSGFQEYLMHLKKKFQRVCVRKPKASRAESREVYLLATGYHPY
                     SKETQGVCLERHA"
     gene            2292..>3433
                     /locus_tag="PK389_03095"
     CDS             2292..>3433
                     /locus_tag="PK389_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HQY22730.1"
                     /translation="MLKNLVLWLVIALVLMSVFSNFGPPQKQVTKLAYSQFLEKVKLA
                     DIKKITVEDKNVNGVTQDDKPFATYIPSKELPESVINALTTNGAIVVGKAPEQPSLLM
                     NIFVSWFPIILFIGVWIFFMRQMQGGGGGRGAMSFGRSRARLLGEDQVKVTFADVAGV
                     DEAKEEVSELVEFLRDPGKFQNLGGRIPRGVLMVGQPGTGKTLLARAIAGEAKVPFFT
                     ISGSDFVEMFVGVGASRVRDMFEQAKKQAPCIIFIDEIDAVGRHRGAGLGGGHDEREQ
                     TLNQLLVEMDGFEGNEGVIVIAATNRPDVLDPALLRPGRFDRQVSVGLPDIRGREQIL
                     KVHMRKVPISDNVDARVIARGTPGFSGADLANLVNEAALFAAKMNKR"
BASE COUNT         1047 a          629 c          820 g          937 t
ORIGIN      
        1 cagaaatttt cagtatcaaa cagctattag ttgtttagag actattgata agttacatga
       61 aaaaagaata atccacaggg atatcaaacc tgaaaacttt atgatgcatg ttcgagatgc
      121 gcagggaaac gtaatagagc ttaaaaatat aaagcctgaa aattttatga ggaatggtca
      181 aaatgatgtt gtagtggttg ttcctattga ttttggttta tcacggcaat tggaagtgga
      241 ccaaacagtt gaaatagcac ctatggtagg taccttcggt tacattgccc cagaagttat
      301 aaatggggga aaagtttcac gcgagacaga catttatgcc cttggtaaaa tgttaaataa
      361 aaatttcgcc attgatattc ctgaaatgta cgctgatgcc tctgcagaaa gaccatcaat
      421 aaaagatgtt gtgacgtatt tgaaaattca agcacagaaa gtgcaagata aagaaccgtc
      481 attaccgtca tttagtgttt ggaaagaagc acagagcaaa acgcagttag acccatctgc
      541 tgcgttagcg aaggctgccg cacaggtatc caaagaagtc gtagctatca cacaattatc
      601 cagcactcgt gagatgccaa caacaacctc gcctactcat gtgctacagt ttagtaatgc
      661 tggaacgact ggaagtatat cgcctttcgc gacgaaagat ggcagaccag ctccccctcc
      721 acctggttac acatcagatg gtaaaaaagc tcccccaccg ccaccgcgtc aagaaaatgc
      781 tggaccggtt atacatcttc caaaagaaga aaattcttgg aaaaaagcat ggggaccggt
      841 tatacatgtt gttgagggtg acccaaatga caacaagcat aaaccagggg gctagtcgtt
      901 ggttagacag aaacttgccc tttttttggc ttatctgtaa acatttttaa tctcctatat
      961 atttgtttgg tgggtataca cctaaaatat ttgatttaat tatgatatgg agccatttta
     1021 atttaaataa ggttagtatg ctttataaca tttttctcag aactaagcta aatttagttg
     1081 ctaaatcagt cttgaaaaag gtataaatag cgttatgcag caaattccaa tgacagcaac
     1141 aggcgtcgaa aaactacgag aagagctaaa gcatctcaag aacgttgtgc gccctagaat
     1201 aattgcggcg attgctgagg caagagggca cggcgatctt aaagaaaacg ccgagtacca
     1261 tgctgcaaaa gaacaacaag cctttacgga aggtcgtatt cgcgatatcg aacataaatt
     1321 atcatacgtg caagtgattg atttaacagg catggctaat aatgggaagg ttgtctttag
     1381 tgcaacggtg accctgctta acctcgatac agatcaaacg atggtttatc aaatcgttgg
     1441 agaagaagaa gctgacttga agaccggtaa aatctcagtc acttctccca tcgcacgggc
     1501 tgttattggt aaatacaaag gggatactgt tgaagtaaca gcacccggtg gtgttgtgtc
     1561 ctatgaggtg tcagaggtta tttactaacg aatacccaaa aacatgacag gtaaatcacg
     1621 ttctaaatca acaaaatcaa cgaaatcaaa aagcagcaga cgctggttag acagacagca
     1681 ttccgattat tacacacaac ttgccagaga aaagggatat cgatcccgat cagcttacaa
     1741 actcctcgaa attcaagaaa aagacaaaat aatatttaag ggtatgagag tggtagactt
     1801 aggggctgcg cctggcggtt ggtctcaagt gcttgttaac catgttggtt cctcaggcaa
     1861 aattgtggcc attgatattc tccctattga atccttaaaa ggggtcgata ctgtatatgg
     1921 agattttcaa aaaacggaaa ccctagagga attgatgttg attttagatg gaaagagtgt
     1981 cgatcttgta atttccgatt tggcccccaa cttaagtggt attgcagtgg ctgatcaagc
     2041 gcgtgtcatg gacttagcgg aaatggcact tgatttcgct gaaagagtac tggttaaaga
     2101 gggagttttt ttaattaagg tgtttcaagg ctcgggtttt caggaatatc tcatgcattt
     2161 aaaaaagaaa tttcagcgag tttgtgtgcg aaagccaaag gcctctcgtg cagaatcgcg
     2221 tgaagtgtat ttactggcga cgggttatca cccatactct aaagaaacgc agggggtttg
     2281 ccttgaacga catgcttaaa aatttggtct tatggttggt tatcgcattg gtgcttatgt
     2341 ccgtatttag taactttggg ccaccccaaa aacaagttac aaaactggcg tattctcagt
     2401 ttttggaaaa agtaaaattg gccgatatta aaaaaattac ggttgaagat aaaaacgtca
     2461 atggtgttac tcaagatgat aagccatttg caacgtacat cccttctaaa gaacttccag
     2521 aatctgtcat caacgcctta acaacgaatg gcgcgatagt ggtcggaaag gcgccagaac
     2581 agccaagctt gttaatgaat atttttgtca gctggtttcc aattattttg ttcatcggtg
     2641 tttggatatt ttttatgcgt cagatgcagg gcggtggcgg tggccgaggc gctatgtctt
     2701 ttggacgaag tcgggcgcgt ttgttgggtg aagatcaagt caaagtcact tttgcagatg
     2761 ttgctggcgt cgatgaggcc aaagaagaag tctcagaact ggtagaattt ttgcgtgacc
     2821 ccggtaagtt tcaaaattta ggcggaagga ttccacgagg cgttttaatg gttggtcagc
     2881 caggaactgg taaaacactg ctagcgcgtg ctattgcagg cgaagctaaa gtgccttttt
     2941 ttacaatttc gggttctgat tttgtagaaa tgtttgtggg tgtgggtgct tctcgtgtac
     3001 gcgatatgtt cgaacaagcg aaaaaacagg cgccctgtat tatttttata gacgaaattg
     3061 atgcagtagg tcgacatcgt ggcgcaggtt tgggcggtgg tcatgatgaa cgtgagcaaa
     3121 ccctcaatca attattggtt gaaatggatg ggtttgaagg caatgaaggc gttattgtga
     3181 ttgctgcaac gaatcgcccc gacgtattag atccggcttt attaagaccg ggtcgttttg
     3241 atagacaagt atctgttggc ttacctgata ttcgggggcg tgaacaaatt ttgaaagttc
     3301 atatgcggaa ggtgcctata tcagacaatg tcgatgcgag agtgattgca agaggaacgc
     3361 ctgggttttc gggagcagat cttgccaacc tcgttaacga agccgcgctg ttcgcagcca
     3421 agatgaataa gcg
//
LOCUS       DAORVT010000117         3415 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_23314_length_3415_cov_3.277679, whole genome shotgun sequence.
ACCESSION   DAORVT010000117 DAORVT010000000
VERSION     DAORVT010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23314_length_3415_cov_3.277679"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PK389_03100"
     CDS             <1..214
                     /locus_tag="PK389_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005368878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HQY22731.1"
                     /translation="YVNILENPEIRAILPSYAKWPTFPQLYIQGELIGGCDIVSELHQ
                     KGELKTLLENAQSLMDAKPEAVLASE"
     gene            241..903
                     /locus_tag="PK389_03105"
     CDS             241..903
                     /locus_tag="PK389_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_820725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HQY22732.1"
                     /translation="MAYLTVVQEVMVWVLPVLFAVTVHEVAHGWVANLRGDSTALMMG
                     RLTLNPLKHIDIVGTVIVPISCLVLGGFVFGWAKPVPVSWRNLRKPRQDAALVAVAGP
                     LSNLLMALLWAFIAKMGVMLFQQGFSSALFLAYMGNAGISINLMLMILNLLPIPPLDG
                     SRVVSSLLPPRLSRSYDLIEPYGLMILVALIFTNILSMILNPPLQWMQIGILRLFGLF
                     GL"
     gene            916..1959
                     /locus_tag="PK389_03110"
     CDS             916..1959
                     /locus_tag="PK389_03110"
                     /inference="COORDINATES: protein motif:HMM:NF021367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HQY22733.1"
                     /translation="MNSGIKKLSLLFFFFLSFSTGLYAVEVQLYQVELSTQNQTEKFD
                     QKKLLKEALKTALVRISGSSQFLNTPEVTNALTNVDGYIKQFSYHQRSALEKTIKVIF
                     NENRVNALLNSVKQSPWNKNRPLTLVWLSGADRDPLIASNMEKILNQRGIPAVFALMD
                     LTDTALVSEQDLVGEGTAALEEAAKRYNPDVILSGRLAETNGIWQTHWRFINKGEKAD
                     WDNSGAKLNVVLDQVADTLALKLKGDKVLVSADQIERPVINHLILTVSGNLDVQQYSK
                     ILKYLRELPGVSEAEVAQIMPDKTLFELVTTQSKAMLINAIADGQILTKTPAAAESTT
                     EEDSMVYEVAGVL"
     gene            1964..2656
                     /gene="hda"
                     /locus_tag="PK389_03115"
     CDS             1964..2656
                     /gene="hda"
                     /locus_tag="PK389_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012569598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HQY22734.1"
                     /translation="MIEQLPLNIQWRDDATLQNFYPGDNVEALKAISELSQGMGEPFL
                     YIWGQAGVGRTHLLQAACHAASEKGIAAFYISLRDQNQLSLSALQGVEDLPLVCIDDI
                     DHIANLSLWEEALFHLFNRIQTKKGRLLIAADVAPKYLPLSLPDLKSRLNWGVTYQLH
                     RLTDEQKVVVLQLRAQLRGLKLNKTVGQFLLSHCSRDMSELQSILEKLDRASLVEQHR
                     LTIPFVKHVLDL"
     gene            complement(2645..3244)
                     /gene="wrbA"
                     /locus_tag="PK389_03120"
     CDS             complement(2645..3244)
                     /gene="wrbA"
                     /locus_tag="PK389_03120"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HQY22735.1"
                     /translation="MQPYILVVYYSRYGATKNMANLIARGIHSVSGIEAKLRTVPPVS
                     ATSEATTSAVPADGDPYVTLEDLKNCAGLAMGSPTRFGNMAAPLKYFIDSTSSLWLSG
                     DLSGKPASVFSSSASIHGGQETTLISMMLPLMHHGAIIVGIPYTESTLITTTTGGTPY
                     GPTHVAGADSKLPISEDEKTLCLALGKRLASITLKLSKI"
BASE COUNT          958 a          620 c          783 g         1054 t
ORIGIN      
        1 gtatgtcaat attttagaaa accctgagat tcgcgcaata ttgccttctt atgcaaaatg
       61 gccaaccttt ccgcagcttt atattcaggg cgaattaata ggcggatgtg atattgtttc
      121 tgaattgcat caaaaaggag aattaaagac gttattagaa aacgcacaat ccttaatgga
      181 tgctaaaccg gaagccgtct tagcctcaga atagcgagta caaatggata tcaatacaaa
      241 atggcgtact taacggtcgt acaggaagtg atggtttggg tattaccggt tttattcgcg
      301 gtaacagttc atgaagttgc acatggatgg gttgcaaatc tgcgaggaga tagcaccgca
      361 ttgatgatgg ggcgtcttac tctgaatccc cttaaacata ttgatatcgt tggtacggtt
      421 attgtaccga tttcatgctt ggttttgggt ggttttgttt ttggttgggc aaaacctgtt
      481 cccgtaagct ggcgaaattt acgaaaaccc agacaggatg ccgcactggt agccgtcgct
      541 gggcctctct caaatttgct gatggcgctt ttatgggcgt ttattgcaaa aatgggcgtt
      601 atgctttttc aacaaggatt ttcctctgcg ctgtttttgg cctatatggg taacgcgggt
      661 atttcgatta atctaatgtt gatgatttta aatttgctgc ctattccacc gttggatggt
      721 agtcgtgttg tttcaagtct tttaccgccg cgtttgtcga gatcttacga tctgatagag
      781 ccttatgggt tgatgatttt ggttgctttg atttttacaa atattttatc gatgatcctt
      841 aatccgccgt tgcaatggat gcaaataggg attcttagac tttttggatt gtttgggttg
      901 tgaggaatgc gcattatgaa tagcggaata aaaaaactgt cgctcttatt ttttttcttc
      961 ttgagttttt caactggact ttatgcggtc gaagtgcagt tatatcaggt agagttatcg
     1021 acacaaaatc aaaccgaaaa atttgatcaa aaaaaattgt tgaaagaggc tcttaaaaca
     1081 gcattagtgc gcatatcggg atctagtcaa tttttaaaca cacctgaggt cacaaatgca
     1141 ttgacgaatg tcgatggtta cattaaacaa ttttcatatc atcaacgttc tgcgcttgaa
     1201 aaaacgatta aggttatttt taatgaaaac cgtgtgaatg cgcttttaaa ttcagttaaa
     1261 caatctcctt ggaataaaaa tcgtcctcta acattagtgt ggttgagtgg tgcagataga
     1321 gatcctttga ttgcatccaa catggaaaaa atcttaaatc aacgtggaat tcccgctgtt
     1381 tttgcgttga tggatctcac tgatactgct ttagtttcgg aacaagatct ggtgggagaa
     1441 ggaactgcgg ctttagaaga agccgctaaa cgatataatc ccgatgtgat tttatccgga
     1501 cgattagcag agacaaacgg aatttggcaa acgcattggc gttttatcaa caagggcgaa
     1561 aaagccgatt gggataattc aggtgctaaa ttaaatgttg tattggatca agttgcagat
     1621 acgttggcgc ttaagttaaa aggcgataag gttctggtgt ctgcagatca aatagaaaga
     1681 ccggttatca accatttaat cttaacggtc tcgggtaatt tagatgtaca gcaatatagt
     1741 aaaatattaa aatatcttcg agaattaccg ggagtttcgg aagcggaagt ggctcaaatc
     1801 atgccagata agaccctatt tgaattagtc acaacccaaa gcaaagcgat gcttattaat
     1861 gccattgcag atggacagat cttaacgaaa acacccgctg ctgccgaatc aacaacagaa
     1921 gaagattcaa tggtttatga agtagcaggg gttttataat cgcgtgattg aacaactccc
     1981 tttaaatatt caatggcgtg atgatgcaac gttgcaaaat ttttatcctg gggataacgt
     2041 agaagcactt aaggctatct cagaattatc gcaaggcatg ggtgagccat ttctctatat
     2101 atggggtcaa gcgggtgttg ggcggacaca tttattgcaa gccgcgtgtc atgctgcttc
     2161 agagaaaggg atcgctgctt tttatatttc attgcgtgat caaaatcagt tgtctttaag
     2221 cgcactgcaa ggagtagaag atttaccctt agtttgtatt gatgacatcg atcacattgc
     2281 aaatctgtca ctttgggaag aggcactctt tcatttattt aaccgaattc agacgaaaaa
     2341 agggcgcttg ttgatcgcag ccgatgttgc gccaaaatat ttaccccttt cgttgcctga
     2401 tttaaagtct cgtttaaatt ggggagtcac gtatcaatta caccgcttaa cggatgaaca
     2461 aaaagtggtt gtattgcaat tacgcgctca acttcgaggt ttaaagttaa ataaaacggt
     2521 cggtcaattt ttattaagcc attgttcgcg cgacatgagc gaattacagt ctattttaga
     2581 aaagcttgat cgtgcttcat tggtagagca acatcgttta accattccat ttgttaaaca
     2641 tgttttagat ctttgataat ttcaacgtaa tacttgctaa gcgtttacct aaagccaaac
     2701 agagtgtttt ttcatcttct gaaataggta atttgctatc agcaccggca acatgtgtag
     2761 ggccataagg tgttccgccg gtcgttgtcg tgattaaagt gctttctgta tagggtatgc
     2821 ccacaataat ggcgccatgg tgcattaaag gtagcatcat gctgatcagg gtcgtttctt
     2881 gtcccccgtg gatactggct gaagaactaa atacagatgc aggtttgcca gataaatcac
     2941 ccgataacca caagctgctt gtgctatcaa taaaatactt cagtggtgct gccatattac
     3001 cgaaccgggt agggctaccc atcgctaaac ctgcacagtt ttttagatct tctagggtta
     3061 cgtaagggtc cccatcggct ggcactgctg atgttgtggc ctcggaggtt gcagaaactg
     3121 gcggtactgt tcttaatttt gcctcaatgc cggagacact gtgtatgcca cgtgcgatta
     3181 aattggccat gttttttgtg gcaccgtagc ggctataata cacgacgaga atgtaaggtt
     3241 gcattttttc tcccggaaat tggcatcagt gtgatattag cacaagggta tttgatatgg
     3301 cgaaaatttg cgtgcattgt tttgtgtcag gtcgtgtaca aggcatgttt tttcgccgag
     3361 aggcacagca acaaagcagt cttggcgtat taaacttggg tttggttaaa aatta
//
LOCUS       DAORVT010000118         3410 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_23378_length_3410_cov_3.217586, whole genome shotgun sequence.
ACCESSION   DAORVT010000118 DAORVT010000000
VERSION     DAORVT010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23378_length_3410_cov_3.217586"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /gene="glyA"
                     /locus_tag="PK389_03125"
     CDS             <1..538
                     /gene="glyA"
                     /locus_tag="PK389_03125"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017376526.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HQY22736.1"
                     /translation="ILSLSNPEIQKKLNSALFPGLQGGPLMHVIAAKAIAFKEALAPE
                     FKAYQKQVIVNARLFAEGLMQQGFKIVSGGTDNHLFLVDLSDRDFTGFDAEKILGDAH
                     ITLNKNAVPNDSRSPFLTSGIRIGTPAVTTRGFKEPEITALISWTADVLNHLKDEIVI
                     QKVKSAVLELCKRFPVYA"
     gene            539..1012
                     /gene="nrdR"
                     /locus_tag="PK389_03130"
     CDS             539..1012
                     /gene="nrdR"
                     /locus_tag="PK389_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020681962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HQY22737.1"
                     /translation="MQCPFCRVDETKVIDSRLVGEGDQVRRRRECISCGERFTTYETI
                     ELSLTRVLKRDGSCVQFREEKLRAGILRALEKRPVRTEQVDAAVNRLLKRLRASGERE
                     IETRVIGEWVMAELRVLDEIAYVRFASVYRHFKNIDEFQAEIDRLKEKVRADSHD"
     gene            1005..1439
                     /gene="nusB"
                     /locus_tag="PK389_03135"
     CDS             1005..1439
                     /gene="nusB"
                     /locus_tag="PK389_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008733467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HQY22738.1"
                     /translation="MTKKISFTGRRKARRYALQALYSWVLSENALSDIETHFLVEHAA
                     EEFDREYFQTLLHEVPRHIEILETTMSPYLSRQFADLDPIELTILRISIYELKERLDI
                     PYRVIINEGLELAKAFGSVDSYKFVNGVLDKVARELRKTEIQ"
     gene            complement(1433..2566)
                     /locus_tag="PK389_03140"
     CDS             complement(1433..2566)
                     /locus_tag="PK389_03140"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HQY22739.1"
                     /translation="MQKYLKAIFISTLALGALSTAYADTRVEGLRYWTTITPLRQTIH
                     LIEVDPAKLSIIPAHAKEKALGRETVSAIAKRYKAIAAINGGFFKSGEWVDGLPAGIL
                     KIHGRWYGIAYRPRGAIGWSNQLYTTLVDRIQTKTNVFLNHKQFPVHAVNQPGLANKS
                     ILYTDAYGPKTDSVPDGTNIIIQNNRIIDIQSSGKIAIPKGGYVFAIGPYAKYPNHPI
                     DIGSLATVNIEIIPHFAKKEHYLAWQMVDNVLGGSPILLYHGKIVQDHSMERLRSPFI
                     TDRYARTAVGVLKNGHWIFAVVEQSALTGSPGMTIAELATFMKDLKCEYALNLDGGGS
                     STLYINNAIINHPGGSDEDDYIWSAVRPVSDAILILPSSTTNY"
     gene            2648..2968
                     /locus_tag="PK389_03145"
     CDS             2648..2968
                     /locus_tag="PK389_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22740.1"
                     /translation="MDTTKLFKGVMSKIMMGASLVLMTTSAYGTSQVDPMVQEARLKA
                     ADGDLEAAVVLYHEAMKKDSENPQIRGELAQIIVKAQVREPHAEVEPEVIEVIEKGTK
                     TSVD"
     gene            complement(2982..>3410)
                     /locus_tag="PK389_03150"
     CDS             complement(2982..>3410)
                     /locus_tag="PK389_03150"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HQY22741.1"
                     /translation="TLQIHHSPEKILILKGDTDNIAMCLKTLKKWLVKQAHTHLIPWL
                     QNLSLDTRLSYHRVTIRGQSTLWGSCNARKNISLNYKLLFLPKTLTEHVLLHELCHLK
                     YLNHSKHFWLFLKSFDPHCTENKKALKLAECYVPAWLRRA"
BASE COUNT         1009 a          611 c          786 g         1004 t
ORIGIN      
        1 tatcttatcg cttagcaatc ccgaaattca gaaaaaattg aattcggcgc tgtttcctgg
       61 tttgcagggc ggccctttaa tgcatgtgat cgcagcgaaa gccatcgctt ttaaagaagc
      121 gcttgcaccc gaatttaaag cctaccaaaa acaagtgatt gtaaatgccc ggttgtttgc
      181 ggagggattg atgcagcaag gatttaaaat agtctctggt ggaacggata accatttgtt
      241 cttagtcgat ttgagtgatc gcgattttac aggctttgat gcagagaaaa tcttaggaga
      301 tgcgcatatt acgctgaaca aaaatgccgt tccgaatgat tcacgttctc cttttttaac
      361 cagcggcatt agaattggaa ccccagcggt taccactcgt ggttttaaag aacccgaaat
      421 tacggcctta atatcctgga cagcagatgt cttaaatcat ttaaaagacg aaattgttat
      481 ccaaaaagta aaatcagcag ttttggagct atgtaaacgt tttccggtgt acgcataaat
      541 gcaatgccct ttttgtcgtg tagatgaaac aaaagtgata gattctcgat tggtgggaga
      601 aggcgaccag gtgcgacgaa ggcgtgaatg tattagttgt ggagagcgtt ttacaaccta
      661 cgagaccatt gaattatctt taacgcgtgt cttaaagcgc gatggcagtt gcgtacaatt
      721 tcgagaagaa aaattacgtg cgggtatttt gcgcgcatta gaaaaaagac ctgtgcgaac
      781 tgaacaagtt gatgctgcgg ttaatcgatt gttaaagcgg ttacgtgctt cgggtgaacg
      841 agaaattgaa acgcgagtga ttggggaatg ggtaatggcg gagcttcgtg tgttagatga
      901 aattgcctat gttcgctttg cttccgtgta tcgccatttt aaaaatattg atgaatttca
      961 ggccgagata gaccgtttaa aagaaaaggt gagagctgat agccatgact aaaaaaattt
     1021 cctttacagg acgcagaaaa gcacgtcgtt atgccttgca ggcgttatat agttgggtac
     1081 tgtctgaaaa tgcattgtcg gatattgaaa cccatttttt agtggaacat gccgcagaag
     1141 aattcgatag agaatatttt caaacgttgt tgcacgaagt gcctcggcac atcgaaattt
     1201 tagaaacgac gatgtcacct tatttaagcc gacaatttgc agatttagat cctattgaat
     1261 taactatttt acggatttct atttatgaat taaaagaacg acttgatatt ccctatcgtg
     1321 tgattattaa tgaaggctta gagttagcta aagcttttgg ttctgtcgat agttataaat
     1381 ttgttaacgg cgtattagac aaagtcgcac gagaattacg aaaaaccgaa attcaataat
     1441 tggtggtgga tgagggtaaa attaatattg catcagagac aggtcttaca gcagaccaga
     1501 tataatcatc ttcgtcgctg ccacccggat gattaataat ggcattattg atgtacaaag
     1561 tcgaagagcc gccgccgtct aaattaagtg cgtattcaca ttttaagtct ttcataaaag
     1621 tggccagttc tgcaattgtc atacctgggc tacccgtcaa cgcgctttgt tctacgactg
     1681 caaatatcca gtgtccattc tttaaaacac caacggctgt acgtgcataa cgatcggtaa
     1741 taaaaggtga gcgcaatcgt tccatagaat gatcttgaac aattttccca tgatatagca
     1801 gtatggggga tccgcccagg acgttatcca ccatttgcca agcaagataa tgttcctttt
     1861 ttgcaaaatg cggaataatt tcgatattga ctgtggctaa gcttccaata tcaatggggt
     1921 gattggggta tttcgcatag ggaccgattg caaaaacata accgcctttt ggaatggcaa
     1981 ttttaccgga ggattgtatg tcgataatgc gattgttttg aatgataata tttgttccgt
     2041 cgggaacaga gtctgttttg ggtccataag catcagtata taaaatagat ttgtttgcaa
     2101 gtcccggttg attaacagca tgtaccggaa attgtttatg gtttagaaag acatttgttt
     2161 ttgtttgaat gcgatcaacc agtgttgtat agagttgatt cgaccagcca atagcgcctc
     2221 gagggcggta agcgatgcca taccatcgtc catggatttt caggataccg gctggcaatc
     2281 catcgaccca ttcaccactt ttaaaaaaac caccattaat ggcagcaatg gctttataac
     2341 gttttgcaat agcactaact gtttcgcgtc ctaaagcctt ctcttttgca tgtgcaggaa
     2401 taatactcag cttagccgga tcgacttcta tgagatgaat agtttgtcta agtggagtga
     2461 ttgttgtcca ataacgtaaa ccctctacgc gcgtgtctgc ataggcagtt gataaagcgc
     2521 ctaaggctag cgtactgata aaaatagctt ttaaatattt ttgcataggt caagctctgt
     2581 gattgattct gcgttagcac agtgtacact ataatatagg gaatgttcta ggacggaaga
     2641 gggctttatg gatacaacca aattatttaa aggcgtgatg agcaaaataa tgatgggagc
     2701 cagtcttgtg ctcatgacca cgagcgcata tggcacgagc caagtggatc cgatggtgca
     2761 agaagcacga ttgaaagccg cggatggtga tttggaagcg gcggttgttt tgtatcatga
     2821 ggccatgaaa aaagatagcg agaatcctca aattcgagga gagctagcgc agatcattgt
     2881 taaagcgcaa gtgcgtgagc cacatgcaga ggtggaaccc gaagtgatag aggttattga
     2941 aaaaggcaca aaaaccagtg tggattaaga caacaaatgt ctcacgcgcg tcttaaccaa
     3001 gcaggcacat aacattcggc taattttaag gcttttttat tttctgtaca gtgcggatca
     3061 aagcttttta aaaatagcca aaaatgttta gagtgattca aatatttgag atgacataat
     3121 tcatgcaata agacatgctc tgtaagcgtt ttgggtaaaa aaagtaattt ataatttaga
     3181 ctaatgtttt ttcttgcatt gcagctgccc caaagggtgc tttgtccgcg gatcgtgacg
     3241 cgatgatagc taagtcgtgt atctaagctc aagttttgta accatggtat taaatgcgtg
     3301 tgtgcttgtt tcactaacca ttttttcaat gtttttaagc acatggcgat attatcggta
     3361 tcgcctttca atattaaaat cttttcgggt gaatgatgta tttgtagcgt
//
LOCUS       DAORVT010000119         3409 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_23388_length_3409_cov_3.408169, whole genome shotgun sequence.
ACCESSION   DAORVT010000119 DAORVT010000000
VERSION     DAORVT010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23388_length_3409_cov_3.408169"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /gene="radA"
                     /locus_tag="PK389_03155"
     CDS             <1..888
                     /gene="radA"
                     /locus_tag="PK389_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002685605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HQY22742.1"
                     /translation="ERILEIALREKPQMLVIDSVQTLYTELLQSAPGSVGQVRESAAQ
                     LVRYAKQTGTALFLVGHVTKEGTLAGPRVLEHMVDTVLYFEGDSDSRFRVIRAVKNRF
                     GAVNELGIFAMTDRGLKEVNNPSAIFLSRYNESVAGSVVMVTQEGTRPLLVEVQALVD
                     ETHLANPRRVTVGLEQNRLALALAVLHRHCGIVTYNQDVFVNIVGGVRVTETASDLAM
                     LLAVVSSLKNRPLPNDLIVFGEVGLSGEIRPVQSGQERLKEAFKHGFKRAIIPYGNAP
                     KTPIAGMEIMAVKRLSEAI"
     gene            complement(898..2577)
                     /gene="ettA"
                     /locus_tag="PK389_03160"
     CDS             complement(898..2577)
                     /gene="ettA"
                     /locus_tag="PK389_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HQY22743.1"
                     /translation="MAQYIFSMHRVGKVVAPNRKILENISLSFFHGAKIGILGLNGSG
                     KSTLLRIMAGIEKEILGEATPQKGIKIGYLHQEPPLDNTKDVRGNVEEGVKEIKALLD
                     RFNEISLKFADPMSDDEMNTLLHEQGELQNKIDAAHGWELDRTLEIAAEALRLPPWNA
                     NVAQLSGGERRRVALCQLLLSTPDLLLLDEPTNHLDAESVAWLERYLHDFTGTVVAVT
                     HDRYFLDNVAGWILELDRGQGIPFEGNYSSWLDQKDKRLKMEERQESAFQRTIKAELE
                     WVRSNPKGRHAKSKARLARFEELNSKEFQKRAETTELYIPPGPRLGDLVIEVNNVSKG
                     FNDKLLFENLNIQIPKGAIVGIIGPNGAGKSTFFKLLTGQEKPDAGSIRFGETVQLAY
                     VDQSRDALNAEKTVWEEISDGQDIMMVGQTEMPSRAYVGRFNFKGSDQQKRVGDLSGG
                     ERNRVHLSKLLKSGGNVLLLDEPTNDLDVETLRALEEALLAFPGCALVISHDRWFLDR
                     IATHILAFEDEGRVSWFEGNYTDYEQDRQLRLGSDALTPHRIKYKKLKTVV"
     gene            2674..>3409
                     /locus_tag="PK389_03165"
     CDS             2674..>3409
                     /locus_tag="PK389_03165"
                     /inference="COORDINATES: protein motif:HMM:NF014623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HQY22744.1"
                     /translation="MRVVAYCTSTGFNINKLAAYLKSTYRATRIREVLHTHIPFKQQT
                     ADKVESEEKGGDVFFFPFGALVCWGLSEDQEQQILEEIRPFEQQRIDLLEEDVFYYIY
                     GEQAKIEDDEIVLPNHDSLTKLAFSHGLAQSVKLSVFEQTIQKTIHLTKELPELLATQ
                     GSIKLSRQDIRRMMGRLFIDRSSINLHQELLDTPEFFWEHSDLESYYALVAHYLDRER
                     RVNVLNQRLRIVQELFDMLAAELNHQH"
BASE COUNT          940 a          683 c          754 g         1032 t
ORIGIN      
        1 gaacgaatat tagaaattgc actacgcgaa aagccgcaga tgttagttat tgattctgtg
       61 caaacactct ataccgagtt attacaatcc gcaccgggtt cggtaggcca agtacgcgag
      121 agtgctgcgc aattggtacg atatgcgaaa caaactggaa ccgcgttgtt tctcgttgga
      181 catgttacca aagaaggtac attagcgggg cctcgcgtac tagaacatat ggtcgatacc
      241 gtgttgtatt ttgaaggcga tagcgacagc cgttttcgcg tgatccgtgc ggttaaaaat
      301 cgttttggcg ctgttaatga gctcggtatt tttgccatga ccgatcgggg tttaaaagaa
      361 gtcaataatc cctcggctat ttttttatcg cgttataatg aatcagttgc gggtagtgtt
      421 gtgatggtaa cccaggaagg cacgcgtcca ctgttggttg aagtgcaagc gttagtagat
      481 gaaacccatt tagccaatcc tcgtcgtgta actgttggtt tggaacaaaa tcgtttggca
      541 ttggcattag cagtattgca caggcattgt ggtatcgtga cgtataatca agatgtattt
      601 gttaatattg tgggtggtgt gcgtgttacc gaaacagcct ctgatcttgc aatgttattg
      661 gctgttgttt ccagccttaa aaatagaccc ttgccaaatg atttgatcgt atttggcgaa
      721 gtgggtttat cgggtgaaat ccgaccagtg caaagcgggc aagagcgatt aaaagaagcc
      781 tttaagcacg gatttaaacg cgcaattatt ccgtatggca atgcacccaa gacacccatt
      841 gcaggcatgg aaattatggc agttaaacgg ctttcagaag ctatataata tagaacgtca
      901 tacgaccgtt ttcaatttct tatatttaat gcgatgcggt gtcagtgcgt ctgaacccaa
      961 tcgcaattgt cggtcttgtt cgtaatccgt atagttgccc tcaaaccaag acacacgccc
     1021 ttcatcttca aaggctaaga tatgtgtggc aattctatcg agaaaccagc gatcatgtga
     1081 aataaccagc gcacatccgg gaaatgctaa taaggcttct tcaagcgcgc gcagtgtttc
     1141 aacatctaaa tcatttgtag gctcatctaa taataaaaca ttgccgccac tttttagcaa
     1201 tttggagaga tgaacgcgat tacgttcgcc gccggagaga tcacccacgc gcttttgttg
     1261 atcagaacct ttaaaattaa agcggcctac ataagcacgc gaaggcattt cagtttggcc
     1321 aaccatcata atatcttggc catcggagat ttcttcccaa acggtttttt ctgcatttaa
     1381 agcatcgcgc gattgatcaa cgtaggctaa ttgaaccgtt tcgccaaatc ggattgatcc
     1441 tgcatcgggt ttttcttgac ctgttaagag tttaaagaaa gttgattttc ccgcgccgtt
     1501 gggtccgata atcccaacaa tagcgccttt tgggatctgt atatttaaat tctcaaataa
     1561 gagtttgtca ttaaaacctt tagaaacatt attgacttcg atgaccagat cgccaagacg
     1621 tggtccgggt ggaatataaa gctcagtagt ttctgcacgt ttttggaatt ctttagaatt
     1681 taattcttca aatcgtgcaa ggcgtgcttt acttttggca tgacgtccct tagggtttga
     1741 tcgcacccat tctaattctg ctttaatggt tcgttgaaag gcagattctt gtcgttcttc
     1801 catttttaga cgcttgtctt tttgatcaag ccaagatgaa tagtttcctt cgaaggggat
     1861 accttgtccg cgatcgagtt cgaggatcca gcctgcaaca ttgtctaaga aataccgatc
     1921 gtgggtaacc gcaaccacag tgcccgtaaa atcatgtaaa taccgctcta gccaagcaac
     1981 gctttctgca tctaaatggt ttgttggttc atctagtaac agtaaatcgg gtgtagataa
     2041 taataattga cacagcgcga cgcgtctgcg ttcaccaccc gataattgcg caacattggc
     2101 attccaaggt ggcaagcgta aagcttctgc tgcaatttcg agtgttctat cgagctccca
     2161 accatgcgct gcgtcaattt tattttgtaa ttcaccttgt tcatgcagta gcgtattcat
     2221 ttcgtcatcg ctcatggggt ctgcaaattt taagctgatt tcattgaaac gatcgagcag
     2281 ggctttgatt tctttgacac cttcttcaac attgcctcga acatctttgg tattgtctaa
     2341 aggcggctct tgatgtaaat aaccaatttt tatgcctttt tgtggtgttg cttctccgag
     2401 aatctctttt tcaatacctg ccataatgcg cagaagggtt gatttacccg aaccatttaa
     2461 acctaggata ccgattttgg cgccatgaaa aaaggacaga gaaatatttt ctaaaatttt
     2521 gcgattaggg gctacaacct tacccacacg gtgcatcgaa aaaatatatt gtgccattga
     2581 cgtactcact taagttaggt atttaaaaag tatctaaaga ctaataactg tgctaggatt
     2641 gtatcacatt caatagggaa acagggctgg tttatgcgtg ttgttgcata ttgcacgtca
     2701 acagggttta atataaataa gttggctgct tatcttaagt caacttaccg tgccactcgg
     2761 atccgggaag tcttgcacac gcacatcccc tttaagcaac agacagcaga taaagtagaa
     2821 agcgaagaga agggtggtga tgtttttttc tttccatttg gcgcgttagt ctgctgggga
     2881 ttatcggaag atcaagaaca acagattttg gaagagatta gaccctttga gcaacaacgt
     2941 atcgatttgc ttgaagaaga tgttttttat tatatctatg gggaacaggc taaaattgaa
     3001 gatgatgaaa ttgttttacc caatcacgat agtttaacta aactcgcatt ttcgcatgga
     3061 ttagcacagt cggtaaaatt gagtgtgttt gaacaaacga ttcaaaagac tattcattta
     3121 acgaaagaat tgccagaact gctcgcaaca cagggcagta ttaagctatc tcgtcaggat
     3181 attcgtcgaa tgatggggcg attatttatt gatagaagtt ctattaattt acatcaagaa
     3241 ctcttagata caccggagtt tttttgggag cattctgatc tcgaatctta ttatgcactt
     3301 gttgcacatt atttagatcg cgagcgtcgt gtaaatgttt taaaccagcg cctgcgtatt
     3361 gtgcaggagt tgtttgatat gttggcagca gaactgaatc accagcatt
//
LOCUS       DAORVT010000120         3406 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_23427_length_3406_cov_3.433602, whole genome shotgun sequence.
ACCESSION   DAORVT010000120 DAORVT010000000
VERSION     DAORVT010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23427_length_3406_cov_3.433602"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..379)
                     /locus_tag="PK389_03170"
     CDS             complement(80..379)
                     /locus_tag="PK389_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HQY22745.1"
                     /translation="MNTRTLLLIVALPMAMLFAASSFAGGKEEKVQWSSVPAVVQKTI
                     TENAGGGKIEEIEKETETKDGKSITVYEAKVKNSDGKKIEIKVGEDGKLIKVEND"
     gene            complement(734..877)
                     /locus_tag="PK389_03175"
                     /pseudo
     CDS             complement(734..877)
                     /locus_tag="PK389_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515356.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(890..2140)
                     /locus_tag="PK389_03180"
     CDS             complement(890..2140)
                     /locus_tag="PK389_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HQY22746.1"
                     /translation="MRSIAVSVILVIFIALLPALTVVSSLSLNVLGFGLLSGWLGTGL
                     IAASLLLMIRDPYWASWFGGLQQMYRWHHGMGVLGFVLLLAHPLLLAGHYLPLDPDIA
                     WEYLSPLNPQSINILGWLALIIFTLGMAATFVLHLPYGMWRRFHMLLVIAIALGLGHI
                     WSVSGFSISLTAALLPAVISLGWRLLRADRGIGARPYEVSAVDHPAEDITEVNLRPLA
                     RPISIAPSQFVSAAFFEGPHFQGCGDFHPYTVSHIAKDGSLTLSIKALGDCTQHIQSL
                     EPGVAVRLQGPYGNFLLDRPIAPEVWIAAGVGLTPFLALLRSQTLTQHTDMFYVHRES
                     ESVPYEEELQRFATNQALLCFHSLSMTNDLTLLFTWLESIDKLNKKQVYLCGPPPFMA
                     KVTKWLREHDMPRQQIHFEQFDFR"
     gene            complement(2296..2649)
                     /locus_tag="PK389_03185"
     CDS             complement(2296..2649)
                     /locus_tag="PK389_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HQY22747.1"
                     /translation="MHYVISTTWGPTDVTRAALPFIFAASALQAEDTVMIMLFHDAVT
                     IAIDGTHQKMIPFGPPSKFAEVFSNSNAKVLVCKPCAEARSIREDMLVKNAIFGGMND
                     LHQDVSRPDAKFISF"
     gene            complement(2794..>3406)
                     /locus_tag="PK389_03190"
     CDS             complement(2794..>3406)
                     /locus_tag="PK389_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22748.1"
                     /translation="KFKKGAMMNEAKTLLVAIRGWFSTGDLALLKRMGILKYMGSEIP
                     DIFTLAITSKLKYVEVLRPELEMDLYSTRSPESLAQDIYNQIEKKISESSDIKSIVLL
                     GYSAGSVLARRVFSMAHGAKTDATIDESKKADWADLIDRIIILSGITRGWEFSSATPA
                     TIRFIGPILEVFLRGFLGLKQLLGNSKKRAVSIPMPLVKFALL"
BASE COUNT         1050 a          753 c          680 g          923 t
ORIGIN      
        1 gactgttata agatgagaac gatcaccaat gctgggctat ggtcaattct cacttaaacc
       61 tcaactgttg cttctgggat taatcgttct caaccttgat gagctttccg tcttcaccga
      121 ccttaatttc aatcttctta ccgtcggagt tttttacctt tgcttcataa accgtaattg
      181 atttgccatc ctttgtctct gtttccttct ctatttcctc aatttttccg ccccctgcgt
      241 tttctgtaat ggttttctga actacagcag gaacgctaga ccattgtact ttctcttcct
      301 ttccacctgc aaaacttgat gctgcaaata acattgccat cggtaaagcg actattaaaa
      361 gaagtgttct agtattcatt gttgatcctt atcttattta gtttaaaaaa tggaaaattc
      421 cacatttaaa agataactat aaaaatagga atattctgtg cggcagagca cataacaaaa
      481 gatagggcta aaagggatca gtgggtgcaa ttaaacagaa acttgtggtt tcggaatagg
      541 tcagcgcaat tcacgcagcc aattgaaaaa aaagatagaa caagtcatgt caataatcac
      601 tgcatgtgtt ttctgagcag gattgtaaga tccagtgaaa actgccactt gaaattattc
      661 aagaccagcc attctgacca ctatagtgtt gatactggtt aaaaatgaat aagccattct
      721 attccgagaa ttacagattg gtagtgatga taacgttggt tggttggttg gttggttggt
      781 tggttcgttc gttgaggtta gaaagcaagt agaatagcaa agcatcaccg gcctgagaga
      841 atggcaagta tccgaattca tctaaaatga ccgagtctat ctatgctcat taacggaaat
      901 cgaattgttc aaaatgaatt tgttgcctag gcatgtcatg ttcccgtagc cacttggtaa
      961 ccttagccat gaatggcggt gggccgcaca gatacacctg tttcttattg agcttatcaa
     1021 tgctttccaa ccacgtgaat aaaagtgtta aatcattcgt catcgacaaa gaatgaaaac
     1081 ataggagcgc ttggttggtt gcaaacctct gcagttcttc ttcgtaagga acgctctcac
     1141 tttcacgatg aacataaaac atatcggtgt gctgggtgag tgtttgactt cgcagcaatg
     1201 ctagaaaagg agttaggcca acaccagcag cgatccatac ttctggcgcg attggacgat
     1261 caagtagaaa gttaccataa ggtccttgca agcgaacagc aacacccggt tcaagtgact
     1321 gtatatgctg cgtgcaatct cccaatgcct taatactcaa cgtcagacta ccatcttttg
     1381 cgatatgact tacggtatac ggatgaaaat caccacaacc ctgaaaatgt ggcccttcaa
     1441 aaaaagccgc acttacaaac tggctgggtg caatcgatat gggtcttgcc aacggccgta
     1501 aatttacctc agtgatgtct tccgctggat gatcgactgc gctgacctca taaggacgag
     1561 cgccaatacc tcgatcagca cgcaataatc gccatcccaa ggatattacc gcaggaagca
     1621 aagccgctgt taggctgatg gaaaatccac tcacagacca aatatgtccc aaacctaatg
     1681 ctatcgctat gaccaataac atatgaaagc gccgccacat accataaggc agatgtagaa
     1741 caaaggtcgc tgccatgcca agcgtaaaaa tgattaatgc caaccagccc aagatattaa
     1801 tcgattgcgg attaagtggg gataaatact cccatgcaat atcgggatcc aatggcaagt
     1861 aatgaccggc tagcaataaa ggatgcgcta gtaacaatac aaaacctagc acacccattc
     1921 catggtgcca tcggtacatc tgttgcaggc ccccaaacca agatgcccaa tatggatctc
     1981 gaatcattaa cagcaaactc gctgcaatca atccagttcc tagccagccc gacaagagac
     2041 caaatccgag tacattcaat gacaatgaag aaacgaccgt caatgctggt agcaaggcaa
     2101 tgaagataac caaaataacc gatactgcaa ttgatctcat aacaaatata ctattttcgt
     2161 tgcatctgtt cggagatttg tttcttaata aggttctaac agaaaatctg agttaagttc
     2221 aactgaatat cgcatcaagg ataatgacaa atgcaccgaa catacacgat tgggcgatta
     2281 agaatgtgga gcgcattaaa aactgatgaa tttcgcatcc ggacgggata catcttgatg
     2341 cagatcgttc attcccccaa atatagcatt cttcaccagc atatcttctc tgatggaacg
     2401 tgcctcagcg caaggtttgc acacgagaac cttagcattc gaattggaaa atacttcagc
     2461 aaattttgaa ggtggaccga aaggaatcat tttctgatgg gttccatcaa tagcaatagt
     2521 tacagcatca tgaaaaagca tgatcatcac cgtatcttct gcttgtagtg cggaggcagc
     2581 aaatatgaac ggtaacgcag cccgcgtgac atcggttggg ccccaagtcg tagaaataac
     2641 gtaatgcata taacctcctg ggtctttgag tgaaaaagag aattattagc gtataacttg
     2701 ttcaaatcct ataaaggatt tgcgcgacta aaaataagca ttctcattgt atacccaacc
     2761 ttgcatttgt gatcactact atcccgttaa ctttcacagt agagcaaatt taaccagtgg
     2821 cattggaatt gaaacggccc gttttttgga atttcctaag agttgcttca atcccaaaaa
     2881 gcctcgcaaa aatacttcca atattggccc gataaatctg atagttgctg gagtagcaga
     2941 agaaaattcc cagccacgag tgataccgga aaggataatg attcggtcaa tcaagtctgc
     3001 ccaatctgct ttcttacttt catcaatagt tgcatcagtt ttggctccat gagccatgga
     3061 gaaaacgcga cgagcgagca cagaacccgc actataacca agcagcacaa tgctctttat
     3121 atcgcttgac tctgaaattt tcttttcaat ctgattgtat atgtcttgag ctaaagattc
     3181 cggtgatcgc gttgaataca aatccatctc aagttcgggt cgaagaactt caacgtattt
     3241 cagcttagaa gtaatagcga gagtaaagat gtctggaatc tcacttccca tatatttcag
     3301 aatccccata cgtttaagga gtgctaaatc ccctgtagaa aaccatcccc gaatggctac
     3361 taagagtgtt tttgcttcgt tcatcatggc ccccttttta aacttg
//
LOCUS       DAORVT010000121         3404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_23454_length_3404_cov_3.206927, whole genome shotgun sequence.
ACCESSION   DAORVT010000121 DAORVT010000000
VERSION     DAORVT010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23454_length_3404_cov_3.206927"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PK389_03195"
     CDS             <1..555
                     /locus_tag="PK389_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22749.1"
                     /translation="TKAPHLEVALTELEKTTRKLKGKIDTSGGGSHFRIEFKQSLAFV
                     DAFEQNPTENKAKEKTAKGTLVRSHGPGKSSRFLPSYSIETFMQTLAKMGVTEPILRA
                     YICAHGKDITRLPSTEQDKARLLANAHIALKKDQSEMLFFLCVYKGKKNQKGDYPALR
                     KLNPVWSGGKEVTQRLIRTVFSFL"
     gene            complement(620..2374)
                     /locus_tag="PK389_03200"
     CDS             complement(620..2374)
                     /locus_tag="PK389_03200"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY22750.1"
                     /translation="MRHIFFSLISGALLMLTACTLPLAPPPTPPTIENDDISSNIKYS
                     EQQDVYCDGKSREQLAFDALVYCANQDDPVAAGRLAEMYLNGEGVVLRNSKVAFYWFS
                     KAAEGGLPYAEIQMGHFYRDGFGVEKDIRRAVCWYEKAAYQGDATAMIALGDLYAKGW
                     HGLKMDREKASYWYCRAASLGSYEAEYRKAWLLVRGKNHHRAEQGLATFIAAAKQGNA
                     EALSDLGDLYAEGRVVRKDLKLSRKYYLSAVEQGSGEAAFKLGMLSEAGGNVSQNRQE
                     AARWFMIAAERDYAPAQLVLGNLYRDGWGLEKNYQEAAFWYTKAADQGIASAESELGD
                     LYHSGRGVTRNLQEAARHYEIAAESGLPYAQLILGILYQDGRGVSEDMVKSMRWYKAA
                     SKQSGFSMAEFRVGRRYATGFGLPKDSKEANRWYTFAANHGLALAEIELAEMYYFGRG
                     MDQNSEEAYRWYHKAAKQGHSYAQYMVGLMILEGNGVAENPAKASIWIRNAAYQGARQ
                     AQYQLGLLYLNGTGIVRSDTQAYAWLKTGLIEQMDQTPDVLKALIDKMTPQSRMRAVA
                     LGEQYTRQYPAVVHSPQF"
     gene            2502..3242
                     /locus_tag="PK389_03205"
     CDS             2502..3242
                     /locus_tag="PK389_03205"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQY22751.1"
                     /translation="MIGKCILATVCWLDLVLATLVLYLLSFLPQHLTKSWYPTLFRHW
                     CWIFIRALGVNLKLHQKNAFPLPKQFILIGNHPSVLEDLGMPALFKARFLAKAELKDW
                     WILGRLSVATQTLYVKRDSRESRKAASEALIRCLAQKENVGLYPEGGCKGRQIYLPFQ
                     YGSFDIAIQTGVSIIPVFLHYEAQEAFEWGSNVSLIHKLWQIWQSPNKNANYYVFDAI
                     DPKKFENKEELCSHVENLYLKWQSKYLE"
     gene            3257..>3404
                     /locus_tag="PK389_03210"
     CDS             3257..>3404
                     /locus_tag="PK389_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020394138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HQY22752.1"
                     /translation="MTRFQQHNHEWYAEGISLNAIAEKIGTPCYVYSRATLEQQWHAF
                     NNAFQ"
BASE COUNT          986 a          810 c          633 g          975 t
ORIGIN      
        1 acaaaagcac ctcatctgga agttgcgctt acagaacttg aaaaaaccac aagaaaactt
       61 aaaggtaaaa ttgacacgtc tggtggcgga tctcatttta gaattgaatt taaacagtcc
      121 ctcgcttttg ttgatgcttt tgaacaaaat cctacagaaa acaaagcaaa agaaaaaacc
      181 gcaaaaggca ctttggtaag atcacatggt ccgggtaagt cttctcggtt tttaccctct
      241 tattccattg aaacatttat gcaaacttta gcaaaaatgg gtgtcaccga acctatttta
      301 agagcttaca tttgcgctca tggaaaagac attacaagat taccctctac agagcaagac
      361 aaagcaaggc tcttggctaa cgcgcacata gcgcttaaaa aagatcaatc tgaaatgcta
      421 ttctttttat gtgtttataa gggcaaaaaa aaccaaaaag gcgattatcc cgctttgaga
      481 aaattgaacc cagtgtggtc aggcggaaaa gaggtaacac aaaggttgat acgaacagtt
      541 ttttcttttt tatagtaaaa aaattatcta aacttttgca gatatccggc cttagtataa
      601 actgcaattc ttaaaataat taaaattgcg gtgaatgcac aacagcggga tactgacgcg
      661 tatactgttc acccagtgcg acagcacgca tccgagactg tggtgtcatt ttatcaatta
      721 gtgcttttaa aacatcgggc gtttgatcca tttgctcaat aagacctgtc tttaaccatg
      781 cataggcctg agtatcactt cggacaatac ccgtgccatt taaatataat aaccccaatt
      841 gatattgcgc ttgtctagca ccttggtaag cggcgtttct aatccatata gaggcttttg
      901 ccggattttc tgccacgcca ttgccttcta atatcattaa accgaccatg tattgtgcat
      961 aggaatgtcc ttgttttgca gctttgtgat accatcgata agcttcttcc gaattttgat
     1021 ccattcctct accaaaataa tacatttcag ctaattcgat ttctgccaaa gctaagccat
     1081 ggtttgccgc aaaagtatac caacgattgg cttcttttga atctttcggt aatccaaatc
     1141 ccgtggcata acggcgccct acccgaaact ccgccatgga aaatccactc tgtttactcg
     1201 ctgctttgta ccatcgcata cttttgacca tatcttccga tacacctcgg ccatcttgat
     1261 acagtatgcc taatatcaac tgcgcatagg gtaaccctga ttccgccgca atctcataat
     1321 ggcgagcggc ttcttgcaaa ttacgcgtta cacctcgccc tgaatgatac aaatccccta
     1381 attcgctttc tgcactcgca attccctgat cagctgcctt agtataccaa aaggctgctt
     1441 cttgataatt tttttcaagg ccccagccat cacggtataa attgcccagt acaagttgtg
     1501 ctggggcata atcacgctcg gccgcaatca taaaccaacg tgcggcttct tgacgatttt
     1561 gtgaaacatt tcctccggct tcagaaagca ttcccagttt aaacgcagcc tcaccggagc
     1621 cttgttcgac tgcggaaaga taatacttac gggataattt caaatcttta cgaaccactc
     1681 gcccttccgc atacaaatcg ccaagatccg acaaggcttc tgcgttacct tgttttgcag
     1741 ccgcgataaa tgttgctagc ccctgctcag cacgatggtg gttttttcct cgaaccaaca
     1801 gccatgcttt tcgatactct gcttcgtaag atcctaaaga agccgcgcga caataccagt
     1861 aactcgcttt ttcgcggtcc attttgagac cgtgccatcc tttagcatac agatcaccta
     1921 gagcaatcat ggctgtcgca tcaccttgat acgccgcttt ctcataccaa caaacggcac
     1981 ggcgaatatc tttttctacg ccgaatccat ctctataaaa atgacccatt tgaatttctg
     2041 catagggaag ccccccttca gcggctttag aaaaccagta aaaggcgact tttgagtttc
     2101 taagcacaac cccttcacca tttaaataca tctcagccaa acgacctgcg gcaacgggat
     2161 catcttgatt tgcacaatac accaatgcat cgaaggctaa ctgctctcgg ctttttccat
     2221 cacaatacac atcttgttgc tcactgtatt taatattaga ggaaatgtcg tcattctcta
     2281 ttgtgggtgg tgttgggggt ggcgctaaag gcaaagtgca agccgtcaac atcaataagg
     2341 cgccactgat tagcgaaaaa aatatatgtc tcatagcaac gtagtatggc attaaatagg
     2401 ctaattgact agttgaggac atgacgatta cttataataa tataattaac ttacttctta
     2461 cttatgcact atctttggac tatccttacc ctatgttcta tatgattgga aaatgcattc
     2521 tggctactgt gtgctggctt gacttagtgc ttgccaccct tgtgctctac cttttatctt
     2581 tcctgccgca acacctaacc aaatcttggt accctacctt attccgtcac tggtgttgga
     2641 tctttattcg agcactcggt gtgaatttaa aactccatca aaaaaatgct tttccattac
     2701 caaagcaatt tatcctcatc ggcaatcacc catccgtctt agaggatctt ggcatgcccg
     2761 ctttatttaa ggcgcgattt ctggccaaag ccgaattaaa agactggtgg atattggggc
     2821 gactcagtgt tgccacccaa actctttatg ttaaacgcga ctcgcgggaa tcacgcaaag
     2881 ccgccagtga agcacttatc cgttgccttg cacaaaaaga aaatgttggg ttataccctg
     2941 aagggggctg caagggccgt cagatctatt tgccttttca atatggcagt ttcgacattg
     3001 cgatacaaac cggagtttcc attattccgg tctttttgca ctacgaagcg caagaagcct
     3061 ttgaatgggg ctcaaatgta tctttaatcc ataaactctg gcaaatctgg caaagtccaa
     3121 acaaaaatgc caattactat gtctttgatg ccattgatcc caaaaagttt gaaaacaaag
     3181 aagaactttg cagtcatgtt gaaaatcttt atctaaaatg gcagtctaaa tatctcgaat
     3241 aatgttagta ttggatatga cacgttttca acaacacaat cacgaatggt atgcggaagg
     3301 gatttcgcta aatgccattg cagagaaaat aggcacccca tgctatgtct attcaagagc
     3361 cactctagaa caacagtggc atgccttcaa taatgctttt caaa
//
LOCUS       DAORVT010000122         3363 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_23976_length_3363_cov_3.667170, whole genome shotgun sequence.
ACCESSION   DAORVT010000122 DAORVT010000000
VERSION     DAORVT010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23976_length_3363_cov_3.667170"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /gene="murA"
                     /locus_tag="PK389_03215"
     CDS             complement(<1..975)
                     /gene="murA"
                     /locus_tag="PK389_03215"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009398241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HQY22753.1"
                     /translation="MGKLIISGGAPLKGEIRISGAKNSVLPILAASLLAGEPVEISNV
                     PHLNDVTTMMELLGQFGAKLTVDENLTIEVDARSVNNYHAPYDLVRTMRASIVVLGPL
                     LARFGEALVSLPGGCAIGSRPVDLHVQGLQAMGAEITIENGYIKARAPKRLHGAAILF
                     DTVTVTGTENLMMAATLAEGTTILKNAAREPEVQDLANFLNVLGAKVSVAGTDTITIE
                     GVPALGGGQYRVVPDRIETGTYLVAAAITRGSIKTKNTKPELLEAVLQKLSEAGADIQ
                     RGEDWISLDMHGRQPKAVNIKTAPFPGIPTDMQAQFTALNAVSDGTGVI"
     gene            complement(963..1289)
                     /locus_tag="PK389_03220"
     CDS             complement(963..1289)
                     /locus_tag="PK389_03220"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HQY22754.1"
                     /translation="MEPTNNIATLEVKNNTLFVSGAIDFDNVVLLWQQGIALIDTLKD
                     TKEIKVDLKALKESDSSGVALLTGWVRVSQEKNKTILLMNMPGFMQDILQVCGLEGVL
                     PVSWEN"
     gene            complement(1303..1962)
                     /locus_tag="PK389_03225"
     CDS             complement(1303..1962)
                     /locus_tag="PK389_03225"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQY22755.1"
                     /translation="MNIKIDVQQKLKRLVLVLGTLGCLLSAPFVFSAAQDPISMLKSV
                     TNNVMQELRTHRQEVQQNSNKLYALVDHLILPHVDFSEMARWVVGRSAWKSADNATQS
                     AFITEFKTMVVRSYARSLLEYTDQKIEFLPLRGAIEDKQRIKVLSLIKENGKDPIHMD
                     YNLIRDGNDWKVYDIIVEGISLMQGYRAQFADDIKQGGLKAAIARMHRHNTQRKSTSG
                     G"
     gene            complement(1959..2483)
                     /gene="mlaD"
                     /locus_tag="PK389_03230"
     CDS             complement(1959..2483)
                     /gene="mlaD"
                     /locus_tag="PK389_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007146986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HQY22756.1"
                     /translation="MMKLRTAEITVGVFMIAGILSLLMLALQVSGLSFFLKEEPGYKV
                     KAEFSNIGALKVRAKVSLGGVVVGRVVKIALEPETFNATVWMTVDPSRVDNLPIDTQA
                     SILTAGLLGDNYISLTPGFNEAEHLKEGDTIPIGSTNSAVILEQILSKFLAGQASKDN
                     SSDNSSNNASGDLR"
     gene            complement(2486..3274)
                     /gene="mlaE"
                     /locus_tag="PK389_03235"
     CDS             complement(2486..3274)
                     /gene="mlaE"
                     /locus_tag="PK389_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008950149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HQY22757.1"
                     /translation="MISLDRVQKLGHTGLHFIGSIGRATLILFNALIGKPDGLRGFSR
                     LIQQLYFVGVLSLLIIVLSGLFIGMVLALQGYNILSKFGAEHALGQLIALSLVRELGP
                     VIGALLFAGRAGSALTAEIGLMKTTEQLSSMEMMGIDPLRRIIAPRFWAGQISMPVLA
                     LIFNCVAILGGYTVGVKWLGVDVGAFWANMQSAVNFNDDIVNGIIKSAVFGTVITWIA
                     VFQGIDCLPTAEGIGRATTRTVVYSSLAILGLDFFLTAVMFGVT"
BASE COUNT         1007 a          830 c          579 g          947 t
ORIGIN      
        1 aatcacacct gtcccgtcgg aaaccgcatt caatgccgta aattgtgcct gcatatcggt
       61 tggaatgcct ggaaaaggtg ctgttttaat attaacggct tttggttgac gaccatgcat
      121 atctagcgaa atccaatctt caccacgctg aatatctgcg cccgcttctg atagtttctg
      181 taagaccgct tctaataatt ccggttttgt attttttgtt ttaatagaac ctctggtaat
      241 cgctgcggca actaaatagg tgcctgtttc tatgcgatct ggcaccacac gatattgccc
      301 cccacctagt gctggcacgc cttcaattgt aatcgtatct gtacccgcaa ccgatacctt
      361 tgcgcctaat acatttaaaa aattcgctaa gtcttgaact tcaggctctc gggcggcatt
      421 tttaagaatc gttgtgccct ctgccagtgt cgctgccatc atcaaatttt ctgtgccagt
      481 gacagtcacc gtatcaaata aaatagccgc gccatgaagc cgtttcggcg ccctggcttt
      541 aatatagcca ttttctatcg taatctcagc acccattgcc tgcaaaccct gtacatgtaa
      601 atcaacgggc ctggaaccaa ttgcacatcc accaggtaac gaaacgagag cttctccaaa
      661 acgtgccaac aatggtccaa gcaccacaat ggaagcacgc atggttcgta ccaggtcata
      721 aggggcatga taattattca ctgagcgggc atcaacttct attgttaaat tttcatcaac
      781 agtcaatttt gcgccaaact gacccaataa ttccatcatc gttgtgacat catttaaatg
      841 tggaacattg cttatctcaa caggctctcc cgctaataac gacgccgcta agattggtaa
      901 aactgagttt ttagccccag aaatacgaat ttcacccttt aaaggtgctc cacctgaaat
      961 aattaatttt cccatgatac tggtaacaca ccctctaacc cacaaacttg taaaatatct
     1021 tgcataaagc ccggcatatt catcaacagt atagtcttgt tcttttcttg tgagacacga
     1081 acccagccgg tcaataaagc aactccacta ctatcacttt ctttcaacgc cttcaaatcg
     1141 actttaattt ctttagtatc ttttagggta tcgattaaag caatcccctg ttgccataat
     1201 agtacaacat tatcaaaatc aatcgcaccc gaaacaaata gcgtattatt tttaacttca
     1261 agtgtcgcga tattatttgt gggttccata agataaacct ttctaacccc cactcgtaga
     1321 cttacgttgg gtgttgtgtc tatgcattct ggcaatagcg gctttgagcc caccttgttt
     1381 aatatcgtct gcaaattgtg cacgatagcc ttgcattaaa ctgatgcctt ctacaataat
     1441 atcgtagact ttccaatcat tcccatctcg aattaaatta taatccatat gaatagggtc
     1501 tttaccattt tctttgatca aacttaaaac ttttatacgt tgcttatctt caattgcgcc
     1561 acgcaagggt aagaattcta ttttctgatc agtatattcc agcaatgaac gcgcgtagct
     1621 tctaacaacc atggtcttaa attctgtgat aaacgcactt tgcgttgcgt tatctgccga
     1681 tttccaggcg ctacgcccaa caacccaacg tgccatttcc gaaaaatcaa cgtgtggtag
     1741 aattaaatga tcgaccaaag catatagttt gttggaattt tgctgcactt cttggcgatg
     1801 cgttcgaagc tcttgcatca cattgtttgt taccgatttc aacattgaaa taggatcttg
     1861 tgcagctgaa aaaacgaaag gcgcgcttaa gagacaccct aaagtcccta aaacgagaac
     1921 caaacgtttc aatttttgtt gaacatcgat ttttatattc atctcagatc tcctgaagca
     1981 ttattcgaag aattgtcgga agaattatcc ttacttgctt ggcccgctaa aaattttgac
     2041 aatatttgtt ctaaaataac agcggaattt gtactgccaa taggtattgt gtcaccctct
     2101 tttagatgtt ctgcttcatt aaaccctggc gtgagactaa tataattatc acctaacaag
     2161 cccgctgtta aaatactggc ctgtgtgtca atgggcaaat tatccacacg actgggatca
     2221 accgtcatcc acacggtcgc gttaaaggtc tccggctcta aggcaatttt aacaacacgc
     2281 ccaacaacta cgccgcctag cgacactttc gcacgcactt tcaacgcacc aatattggaa
     2341 aactccgcct ttactttata gccaggctct tcctttagaa aaaagcttaa accactgact
     2401 tgcaatgcaa gcatcaataa agaaaggata cccgcgatca taaatacgcc gacggtaatt
     2461 tctgctgttc tcaatttcat cattattaag tcactccaaa catgacagct gttaagaaaa
     2521 agtccagacc aagaatagcc agtgatgaat agacaaccgt acgggttgtt gccctaccaa
     2581 tgccttcagc ggttggcaaa caatcaatcc cttgaaacac cgcaatccat gttatcacag
     2641 tgccaaatac agcacttttt ataattccat tcacgatatc atcgttaaaa ttgacagcgg
     2701 actgcatgtt tgcccaaaaa gcacccacat ccactcctaa ccatttcaca ccgacagtgt
     2761 aaccacccaa aatagcgacg caattaaata ttagagccaa tacgggcatt gatatctggc
     2821 ccgcccaaaa tcttggtgca ataatacgac gcaatggatc gatgcccatc atttccatcg
     2881 aagataattg ttcggttgtt ttcattaatc caatttcggc agttaaagca gaaccggcac
     2941 gccctgcaaa caataaagcc ccaatcacag gacccaattc tcttaccaga cttaatgcga
     3001 ttaattgccc caatgcgtgt tcggcaccaa atttagacaa aatgttatag ccttgcagtg
     3061 ccaagaccat cccaataaaa agacctgaaa gaacaataat caagagagaa agcacgccta
     3121 caaaatacag ctgttgaatt aagcggctaa atccacgaag cccatccggc tttccgatca
     3181 atgcattaaa taaaattaaa gttgcacgtc ctatactacc tataaaatgc agacctgtat
     3241 gacctagctt ttgaacacga tccaaagaaa tcatttgcga acctcccaca aatcttcagc
     3301 atatgccttt gcaggataat gaaagggaac aggtccatct ggtaatccgt gtataaactg
     3361 gtt
//
LOCUS       DAORVT010000123         3357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_24046_length_3357_cov_3.383707, whole genome shotgun sequence.
ACCESSION   DAORVT010000123 DAORVT010000000
VERSION     DAORVT010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24046_length_3357_cov_3.383707"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PK389_03240"
     CDS             complement(<1..391)
                     /locus_tag="PK389_03240"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HQY22758.1"
                     /translation="MRRVFLLSKLHKAKVTGADLQYEGSFGIDQELMEAADILPNEQV
                     HVYNVNSGTRFSTYAIPAPYGSRIMCANGACAHLVSLGDRVIICTYGGLEEKEYKTFK
                     PKVLLLDEKNNFIIKNDENLVEELEEAY"
     gene            613..2430
                     /locus_tag="PK389_03245"
     CDS             613..2430
                     /locus_tag="PK389_03245"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HQY22759.1"
                     /translation="MLTAATAPVDEEGQADDDGDKSPVALVDTTRISPHALIRQQLIK
                     TTLPLVMVGVFGTVEDFFRIKFISELGSDALAVDALQGITFGFLLASETGFLNSATMM
                     MAQSFGKGKKEEVGQTAQQGWILGAALSIPTMGVLWFVEPILNLFGQDPKLTAMVSPF
                     ARAVAFASPAIYWTVIDTAFLNAISKQYALVPLFFSSAAFGIGMSYYLLPEVGIVGSG
                     YTILAQAWLRWAALKGYFLLGDFKDYGLFKVRFQGLSHLKKMATYGSPIAALSLGDQI
                     RYFVTGMMNGWLGSEQLAINQSANLYLRFLTPPILGIRQASQILIAQYKGRNDYQNVR
                     RFGKMGIYMEMGINTIPLFAYSLFSMQLATYIVPKQELEGLESFVRWVFITKAISRML
                     ESASIPLSENLKGLLDTCFPSIVQLISSLAVILPLSYVFGFVFDWELTGLNVAVGVGA
                     AVNIPSLLWRWCKLTYPAESANSQESNARLSLSKDGIIPDSDAIIHETEAKKFVAYGL
                     NEENYVTTAHPIAVTDNEPSLPISPASRLLTLSSTSEREAKRATYAPIDPSPSMLSAY
                     AYKSMRNRKHSSSDLDPIPPTSSSSNQRFRRLTQICTIS"
BASE COUNT         1025 a          659 c          630 g         1043 t
ORIGIN      
        1 cgtatgcttc ttcaagttcc tcaactaagt tttcgtcatt ttttattata aaattattct
       61 tttcatccag taacaatact tttggtttaa acgtcttata ttctttttct tctagccccc
      121 cgtaagtaca tatgataacg cgatctccaa gagatactaa atgtgcacaa gcaccgtttg
      181 cacacattat cctggaacca taaggggcag gaatagcata agttgagaat cgagtaccgc
      241 tattcacatt gtaaacatgt acctgctcat ttggtaaaat atccgcagct tccataagtt
      301 cctgatcaat cccaaaagat ccttcgtact gcaaatcagc accagtaacc ttagccttat
      361 gtaatttaga caaaagaaat acccttctca taacacagtt ccttaactaa taaaataagt
      421 aggttataga attttaaaaa agaaatcaac cctaattcat ggtgggtcta atttattgtg
      481 tgtttctttt aattatatct gttcataaaa aataccttga aatttcaaaa ttacttttta
      541 atcgcacaag aatggattaa cataattaaa acttatggtt gaccaggtga cttgaactta
      601 aggagaatat ctatgctgac agctgcaacc gctccagtcg atgaagaagg acaggctgac
      661 gatgacggtg ataagtcacc cgttgctctt gttgacacaa cacgtatttc gcctcacgct
      721 ctcatcagac aacagttaat aaaaacaaca cttccattag ttatggtagg cgtttttggt
      781 actgtagagg atttttttag gataaaattt atttcagagt taggatcaga tgctttagcg
      841 gtagacgctc tacaaggcat cacctttggc tttttactcg cgagcgaaac aggttttcta
      901 aattccgcaa ccatgatgat ggctcagtcg ttcggtaaag gaaaaaagga agaggtgggt
      961 caaactgctc agcaaggatg gattctaggg gctgctctta gtattccaac tatgggcgtg
     1021 ctatggttcg tggaaccgat attaaacttg tttggccaag atcccaagct aaccgcgatg
     1081 gtttctccat ttgcaagagc cgttgctttt gcgagtccag caatttattg gacagttata
     1141 gacacagctt ttttaaatgc aatttctaag cagtatgcct tagttccatt attttttagc
     1201 tccgcagcat ttgggattgg aatgagctac tatctcctgc cagaagttgg aatagtaggt
     1261 tctggctata caattcttgc tcaagcctgg ttgcgctggg cagccttaaa agggtacttt
     1321 ttactcggag attttaaaga ctatggttta tttaaggtgc gttttcaagg cctgagccat
     1381 cttaaaaaaa tggcaactta tggctctcca atagccgcat tatctttggg agatcagatt
     1441 cgctattttg taacaggtat gatgaacggg tggctaggca gtgaacaatt ggccattaac
     1501 cagtcggcaa atttatattt gagattttta acacctccta tcttagggat aaggcaagcc
     1561 agtcaaatac taatcgcaca atacaaaggg cgcaacgact atcaaaatgt ccgacgcttt
     1621 gggaaaatgg gcatttatat ggaaatggga atcaatacga tcccgttatt cgcctacagc
     1681 ctattttcaa tgcaattagc gacttatatt gtaccaaaac aagagctgga ggggttagaa
     1741 tcttttgtga ggtgggtttt tattactaaa gctattagca gaatgttaga atcagcatca
     1801 atccctcttt ctgaaaatct gaaaggctta ttagacacat gttttccttc cattgtccag
     1861 ctaatttcgt ccttggcggt catattacca ttatcttatg tatttggctt tgtatttgac
     1921 tgggaattga ccggactcaa tgtcgctgta ggcgttgggg cagcagttaa cataccttct
     1981 ttactctggc gttggtgcaa actaacctat cctgcagaat cagcgaattc acaagaatcc
     2041 aatgctaggt tgtcattatc taaagacggt ataatacctg actctgatgc tataattcat
     2101 gagaccgagg caaaaaaatt tgttgcctat ggtttaaatg aagaaaatta tgtgacaaca
     2161 gcccacccaa ttgctgttac tgataatgaa ccatcccttc caatatcgcc tgctagtaga
     2221 cttttaacat tatcatctac aagcgagcgg gaagcaaaac gcgctactta tgctccaata
     2281 gatccatcac catcaatgtt atcagcatat gcgtataagt ccatgcgaaa tcgcaaacac
     2341 agttcatcag acttagatcc tataccgccc acctcatcat ctagtaatca acggttccga
     2401 cgtttgaccc aaatatgcac aatttcgtga tctctttttt atcggtattg acaaattaac
     2461 aggaaacttt acaaaagaca tatatataat caacgctttt tatctgctca aaaaaaattt
     2521 cctaatgggt aaatttttcc ccaaacatct gatatttggc attcataatg ctcaatacca
     2581 ttgaaaaaga aaagccaatc aattacgtgt gagcagcagc tgagatgata tagtgtttaa
     2641 catgatcaaa attgtgatcc atattgtatc cgcgattttt tagaatattc ttgttttcat
     2701 ggtaagttga agcgtcttgt tgaaatgtcg ccaaaccgga tacaactcta attgactgta
     2761 ttatttaacc atccccgaag agtggaatgt ataatcgagc aatgcaagat tagatccgtg
     2821 cgattggcaa atcgttcttt ctcgtagttg catcgtttct tctcaaagct catgacaagt
     2881 tgaacggcta aacacattat attacaaaat tttccgtaaa ttatagattc gagactgcat
     2941 caaagtgatc gttttttgag tgttataatg aaatttggat aaaatatatt gtccgcatga
     3001 tttaagtgtc gttttgtact gccgatgtga aatgcataaa caaacataag tttagtgccc
     3061 ttaaacagta gcggtggtcg gaaaatgtac ctagaaaatc aaacgactta tttggatcgt
     3121 agaagcatta gagagggttc tgtgatctag aaaattccag acagcaagca aaatatcggt
     3181 tatggcaaag ccatccgtgc tcaaaaaaat cgatttttta aacggcgtgc cccaaaatcc
     3241 tgcctttcta gtaattcttt tttaccccat tgtcaataca caaccttttt cggtatcatt
     3301 aaaccttatc tctgaaataa gttagtggag ggtccatgca aaatttatgt cggatac
//
LOCUS       DAORVT010000124         3337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_24301_length_3337_cov_3.853138, whole genome shotgun sequence.
ACCESSION   DAORVT010000124 DAORVT010000000
VERSION     DAORVT010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24301_length_3337_cov_3.853138"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1418
                     /locus_tag="PK389_03250"
     CDS             <1..1418
                     /locus_tag="PK389_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22760.1"
                     /translation="KLVVEQSENTSNLLTSLNPSILPLALHKIIQHFTQIILISYELY
                     FTPPTGVWKELHLIYQYAEKKSLLQNNHLENEYKRVLILAATYPYQRRQNQQVPVYKA
                     TEGWATKTTLGRDLPNALDPGFLVIDFEQDKPPIFLSHDLIKFSSSCKVLDVHFLLRH
                     LKTLLTEIEPNELQARMTHHQEPEYAISSAVLKKIIKGWEMPLIRSNDRTTSDKKAQV
                     SIGLLSTHFYLNGQRPFQQQATNEGSENFTLTFSTLSLQENGLEMTKPNSAADQISAL
                     IPETEKTISYPLYPCALISESPHGYGLLWPGNSYPPMQSGEIIGLAIDEPENKTWEIC
                     AIRWLQHLAKTDFKIGVERLSKTATAAAAQLIKDSKPSGYCARCLILESSLLLPAFPF
                     KTGNHIILISENQPMLEVELKKLIDSTGVYNQFQYITKNVPTKTPSTTPTSTPSTTPS
                     FISQKGTEKNDDIFDSVWSDL"
     gene            complement(1452..2120)
                     /gene="rpe"
                     /locus_tag="PK389_03255"
     CDS             complement(1452..2120)
                     /gene="rpe"
                     /locus_tag="PK389_03255"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HQY22761.1"
                     /translation="MQPFIIAPSILSADLARLGQESETVLAAGADWIHFDVMDNHYVP
                     NLTFGAMVCASLRQYGIKAPIDVHLMVKPVDTLIQRFAKAGASTITFHPEASDQVDRS
                     LQLIKDLGCKAGLAFNPDTPFKVFPDMLEKLDMILLMSVNPGFSGQKFMPSVLKKIRE
                     VRTFIDASGKKIRLEVDGGINLTNIKAIATAGADTFVSGSAVFGANDYAVILNALRDK
                     LREH"
     gene            complement(2122..2685)
                     /locus_tag="PK389_03260"
     CDS             complement(2122..2685)
                     /locus_tag="PK389_03260"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HQY22762.1"
                     /translation="MCVRFFAMLVLITACLTSCLTVAENARTGCSVASGYGAADIVPL
                     RLGLQREFEKQWRTESTWPITGYWEGSLYAMHGRKGPLPNSHKQLNAGALAAAVRFSR
                     ALCTTVGWPYVELGIGLSLLSQKEIGGRNLGMHFQFEDRFGIGVRFGENREYDFGYKA
                     VHFSNAYLGSVNHGINLHLLTIGYWFK"
     gene            2856..>3337
                     /locus_tag="PK389_03265"
     CDS             2856..>3337
                     /locus_tag="PK389_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQY22763.1"
                     /translation="MPRKNDKRDRLIDAANDLIYQQTFNATTLADIAAKANVPLGNVY
                     YYFKTKDDILKAVIQKRSVELQAQFSAFEEAYPNAKDRLRALIESNVKTSETTAQYGC
                     TLGSLCQELAKFTGELSQIAAELMHRTLEWVEAQFKAMGKGEQSTILAKYLIGSLQGV
                     S"
BASE COUNT         1103 a          774 c          564 g          896 t
ORIGIN      
        1 ataaactggt tgttgagcaa tcggaaaata cgtcaaattt attgacatca ctgaacccca
       61 gcatcttgcc ccttgcatta cacaaaatta ttcaacactt tacccaaata attttgatta
      121 gctacgaact ctattttacc cccccaacag gcgtgtggaa agaactacat ctgatctatc
      181 aatatgccga aaaaaaatct ctattacaaa ataatcatct ggaaaatgaa tataaacgcg
      241 ttttaatatt ggctgcaact tatccttacc aacgacgaca aaaccagcaa gtgcctgttt
      301 acaaagcaac agaaggctgg gcgactaaaa ccaccttagg tcgagatctg ccaaatgctt
      361 tggatccggg ctttttagtg attgatttcg aacaagataa accacctatt tttttatcac
      421 acgaccttat aaaattttct tcttcttgta aagtattgga tgtacatttt ttattacgtc
      481 atttaaaaac actcttgacc gaaatcgaac cgaatgaatt gcaagccaga atgacgcatc
      541 accaagagcc agaatacgcc atctctagcg ctgttttaaa aaaaataatc aaaggttggg
      601 aaatgcctct tattcgttca aacgacagaa caacatccga caaaaaagca caagtttcta
      661 ttggtctact atcgactcat ttttatctaa acggacaacg tccctttcaa cagcaagcga
      721 ctaatgaagg atctgaaaat tttactttaa ctttttcaac actaagcctt caggaaaacg
      781 gtctagaaat gacaaaaccg aatagtgcgg cagatcaaat atccgcctta ataccagaaa
      841 cagaaaaaac aatttcttac ccactttatc cgtgtgcctt gatcagtgaa agccctcacg
      901 gttatggttt actctggcca ggaaactctt atccacccat gcaatctgga gaaatcatcg
      961 gtttagcaat agatgagcca gagaacaaaa cttgggaaat ctgcgcaatt cgatggttac
     1021 aacatcttgc aaaaacggat tttaaaattg gcgtagagcg attatctaaa actgcgacag
     1081 cagctgcagc gcaactgatt aaagacagca agccatcggg atattgcgca cgctgcttaa
     1141 tactagaatc ctcgttatta ctgcctgctt ttccattcaa aacaggaaat catattattt
     1201 taatctccga gaatcagcca atgctagaag tagagttaaa aaagctcatc gattcgactg
     1261 gcgtttataa tcaatttcag tatattacga aaaatgtacc tacgaaaacg ccttccacca
     1321 cacccacatc aacaccatca accacgccat ctttcatatc tcaaaaagga accgagaaaa
     1381 atgatgacat ctttgattct gtttggtccg atctctaaag ttctacctag aaacaacttg
     1441 ttttttactg gctaatgttc tcttaattta tctctcaatg cattcaatat aacagcgtag
     1501 tcatttgcgc cgaaaacagc agaacctgaa acaaaagtgt ccgcacctgc tgttgcaata
     1561 gctttaatat tcgttaggtt aataccacca tcaacctcta aacgaatttt ttttccactt
     1621 gcatcaataa atgtacggac ttcgcgtatc ttttttaaca cgctaggcat aaatttttga
     1681 cccgaaaacc cagggttcac tgacatcaat aaaatcatat ccagcttttc taacatatca
     1741 gggaacacct taaaaggcgt gtctggatta aaagccaaac ccgccttgca acccaagtct
     1801 ttaattaatt gaaggctgcg atcaacctga tcactggctt ctggatgaaa tgtaatcgtg
     1861 ctggcccctg cttttgcaaa tctctgaatt aaagtatcca ccggcttcac cattaaatgc
     1921 acatcgatag gtgctttgat gccatattgt cttaaggatg cacagaccat tgccccaaaa
     1981 gtcaaattag gcacgtagtg gttgtccatc acatcaaaat ggatccagtc tgcgcctgct
     2041 gctaatacag tctcactctc ttgacctaat cgagccaaat cggcagataa tatcgaggga
     2101 gcaataataa aaggttgcat attacttaaa ccaatagcct atcgttaaga gatgaagatt
     2161 aataccatga tttacagatc ccaaataggc atttgaaaaa tgaacggctt tatacccaaa
     2221 atcatactca cgattctctc caaatcgaac accaatgcca aatctatctt caaactgaaa
     2281 atgcataccg agatttctgc caccgatttc tttttgcgat aaaagcgata atcctattcc
     2341 aagttccaca taaggccaac ctaccgttgt gcaaagcgct cgactgaatc taaccgccgc
     2401 tgccaatgcg cctgcattta gctgcttatg gctattgggt agtggacctt ttcttccatg
     2461 cattgcatat agactccctt cccaataacc cgtaataggc caagtgctct cggttcgcca
     2521 ctgcttttca aactctcttt gcaaacccaa acgcaatggc acaatgtctg cagcaccgta
     2581 cccagaggct actgaacacc ctgtgcgagc attctcagca acagttaaac aacttgttaa
     2641 acaagctgtt ataagaacca gcattgcaaa aaaacgaacg cacataagac actccactga
     2701 aggttgttca aacccctgtt ttctatctta aacgaaaatt caagtttcgt agagacaagg
     2761 cgattaactt atgttaagct tactccctgc tcaatctccg agcaaaattt atctggttaa
     2821 ctcatacagg agcgcactac aacatgtcta gcatcatgcc aagaaaaaac gataaacgcg
     2881 atcgacttat tgatgccgcc aacgatctga tctatcaaca aacgtttaac gctacgacac
     2941 ttgcagatat tgcggccaaa gcaaatgttc cactcggtaa tgtctattat tatttcaaaa
     3001 caaaagatga tattttaaaa gcggttatac aaaaaagatc ggttgaacta caagcacaat
     3061 tctctgcttt tgaagaagca tatcccaatg caaaagatcg tcttcgcgca ttgattgaga
     3121 gtaacgtgaa aacgtcagaa acaaccgcac aatacggatg cacattaggg agtctttgcc
     3181 aagaactcgc caaatttaca ggcgaactgt cacaaattgc agcagaactg atgcacagaa
     3241 ctttagaatg ggtggaagca caatttaaag cgatgggcaa aggcgaacaa tccactatat
     3301 tggctaaata tttaattggc agcttgcaag gtgtgtc
//
LOCUS       DAORVT010000125         3331 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_24368_length_3331_cov_3.137057, whole genome shotgun sequence.
ACCESSION   DAORVT010000125 DAORVT010000000
VERSION     DAORVT010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24368_length_3331_cov_3.137057"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            284..793
                     /locus_tag="PK389_03270"
                     /pseudo
     CDS             284..793
                     /locus_tag="PK389_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841388.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
     gene            851..3022
                     /gene="uvrD"
                     /locus_tag="PK389_03275"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             851..3022
                     /gene="uvrD"
                     /locus_tag="PK389_03275"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HQY22764.1"
                     /translation="MSTSLAALLDPLNIAQQEAVAAPFGNLLVLAGAGSGKTRVLVHR
                     IAWLISQKGAPASSILAVTFTNKAAAEMRGRLEQMLGRDAQGMWVGTFHGLSHKILRL
                     HWQEAGLPEAFQVIDGEDQLQLIRRLFKTLGVNEDRHEPKKIQGFINRKKDEGIRANQ
                     LLSAESGFEQVNHDVYLHYERLCNESGLVDFAELLLRAYEVLKTNAELLAYYQARFLH
                     VLVDEFQDTNGIQYLWLSLLAGQSKSVTVVGDDDQSIYGWRGAKVENIRRFEKEFAGT
                     QLIRLEQNYRSTSTILAAANALISYNGGRFGKTLWTEGEKGDPIIVYAGFNEEDEALF
                     VVREIREWIAEGGKSSEVGVLYRSNAQSRVLEEAFVRAGIPYTIYGGLRFFERAEIKD
                     ALAYLRLVVNCKDNTSFDRVVNVPPRGIGEQTLKKIEAIAEEYHCGLWDAAKKSLEMG
                     LITSRAASGLSTFIALVEKLMANVVGGALTNLIEAVIRESGLLAFYKEQKGEKAQNRI
                     ENLEELINAATDFEAEYPNDGENSPLLAFLSYTALEASDRKNGHLADSVQLMTLHSAK
                     GLEFPLVFICGLEEGLFPHHFSRENPEALEEERRLCYVGVTRARKKLFLTHAEKRRVF
                     GRDEERRPSRFIKEIPEHLLKETNRRVSIRHHAIVDYSVGDDEVDIYGFCMGQRVRHP
                     KFGQGTVVDHEGKNDRARVHVRFDVYGSKWLALAYANLEAV"
     gene            complement(3034..>3331)
                     /locus_tag="PK389_03280"
     CDS             complement(3034..>3331)
                     /locus_tag="PK389_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQY22765.1"
                     /translation="GYSSLSSLKKFPVDSLKIDQSFIRNINHANKALDDGAIITAIIA
                     LAKQLKFKVIAEGVETEAQFNFLHKEGCDLIQGYLFGKNLPEDEFTALLTENRK"
BASE COUNT         1002 a          582 c          778 g          969 t
ORIGIN      
        1 acctacataa aagtttatta tacgcgcatg gctggaaaac tcggcgaaga ttttttaggg
       61 gttttaagag cgtgtgggtt ggatatgaat acattaactc ttcgaaaatc gcgctgaaca
      121 agtagaatgg ctgatgcgaa aatgagaaaa aatttgaaga gatttgcttg tacagtattt
      181 ttatacagtg attattggta gagcgagagc gcttttgatc aaatgtgcat ctaaattgtt
      241 atcatcacgc acttattttt gatatgctga acacatgtta aaaaatgtaa ttttgaaaag
      301 tgctcaattc gattcgccgc tcggcgctat acttgccatt gccgatgacg aggcgttata
      361 tctgttagaa tttgtagatg ctcgcggtgt ggcacgtgaa gtggaaaatc ttcgcatcaa
      421 aataaaagca gagattatac caggcagtgc taatcccatt agatctatca aaaaagaatt
      481 aaaagcttat ttttcaggcg atttaaaaat tttcaaaacg cccgttcaac ttttaggtac
      541 accctttcag caattggttt ggtcggcgtt gatgcgcatt tctcatggtg aaacgagaag
      601 ttatgcagaa caagccatcg cgattaaaaa accaagggca ttcagagccg ttgccaatgc
      661 gaatggtgcc aatcagctgg ccattatcgt tccctgtcat cgaattatta atacgagtgg
      721 agcactgggc gggtatggag gcggcattaa gcgcaaaaaa tggctgttgg atcatgaaaa
      781 acatttatgt taaaaataga atgaatttgg gctatttcta aatccatttt attgttagaa
      841 taagacctat atgagtactt ccttggcagc cttacttgat ccgttaaata tcgcacaaca
      901 agaagcggta gcggcgcctt ttggcaattt attagtgctt gcaggtgcag gcagtggtaa
      961 aacacgtgtt ttggtgcatc gtattgcttg gcttatcagt cagaaaggcg cgcccgccag
     1021 cagtatttta gcggtaacct ttaccaataa ggccgcagca gaaatgcgcg gacgcctaga
     1081 gcagatgttg ggcagagacg cacaaggcat gtgggttggc acttttcacg gtctatccca
     1141 taaaatttta cggctacatt ggcaagaggc gggtttaccc gaagcatttc aggttataga
     1201 cggcgaagat cagttgcaac ttatccgccg cttgtttaaa acattgggcg ttaatgaaga
     1261 tcgtcacgag cctaaaaaaa tacaagggtt tatcaatcgt aaaaaagatg aaggcattcg
     1321 tgcaaatcaa ttattaagcg cagaaagtgg ttttgaacaa gtcaaccacg atgtctattt
     1381 gcattatgag cgtctttgta acgaaagtgg cttagtcgac tttgcagaac ttttattacg
     1441 tgcttacgaa gtgttaaaaa cgaacgcgga actgttagct tattaccaag cccggttttt
     1501 gcatgtatta gtcgatgaat ttcaagatac caacggcata caatatctct ggttgtcgtt
     1561 attagcaggt caatctaaat cagtcaccgt tgtgggagac gatgatcaat caatttatgg
     1621 atggcggggc gctaaagtag aaaatattcg gcgttttgaa aaagaatttg ctggtacaca
     1681 attaattaga ctagagcaaa actatcgttc gaccagcacg attttggcag ccgcaaatgc
     1741 tttaatttct tataatggcg gccgatttgg aaagaccctc tggacagagg gtgaaaaagg
     1801 cgaccccatt atcgtgtatg caggatttaa cgaagaagac gaagcattgt ttgtggttag
     1861 ggagatccgt gaatggatcg cagagggcgg aaagtcaagc gaagtcggcg ttctttatcg
     1921 atcaaatgct cagtcacgtg tgttagaaga ggcttttgta cgcgcgggca taccttatac
     1981 gatttatggt ggcttgcgct tttttgaacg cgcagaaatt aaagatgcat tggcttattt
     2041 aagacttgtt gttaattgca aagacaatac ttcatttgat cgcgttgtta atgtcccacc
     2101 gcgaggtatt ggcgagcaaa cattaaaaaa gatagaagcg attgcagaag aatatcactg
     2161 tggtttatgg gatgctgcaa aaaaatcttt ggaaatgggg ttaatcacca gtagagcggc
     2221 ttcagggttg tcgaccttta ttgcattggt cgaaaaactt atggccaatg ttgttggtgg
     2281 tgccttaaca aacttaattg aagcggttat ccgagagagt ggattattag cgttttacaa
     2341 agaacaaaaa ggtgaaaaag cgcaaaatcg catcgaaaat ttagaagaat tgatcaatgc
     2401 tgctacggat tttgaagcgg aatatccaaa tgacggcgag aattctccgc tgttagcttt
     2461 tttatcatac acagcgctag aagcaagcga tcgcaaaaat ggccatttag cagacagcgt
     2521 gcaattgatg acattacatt ctgcaaaagg gctagaattt ccattggttt ttatttgtgg
     2581 tttagaagag ggtttatttc cacatcattt ttcacgcgaa aacccagaag cgttagaaga
     2641 agagcggcgt ttgtgttatg tgggcgtaac acgtgctaga aaaaaattat ttcttacgca
     2701 tgcagaaaaa cgacgcgtat ttgggcgtga tgaagagcgt cgtccttctc gatttatcaa
     2761 agagatccca gaacatttac tcaaagaaac gaataggcgt gtaagcattc gacatcacgc
     2821 cattgtagat tattctgttg gggatgatga ggttgatatt tatggatttt gtatggggca
     2881 gcgtgtgcgg catcccaagt ttggacaagg tacagtggtt gatcacgaag gtaaaaatga
     2941 tcgcgcgcgt gtacatgtgc gatttgatgt gtatggcagt aagtggttag cattggcata
     3001 tgccaactta gaggcggttt aaagaaaaga gagttacttc cgattttcag ttagtaacgc
     3061 tgtaaattcg tcttctggca agtttttccc aaacaaatac ccttggatca aatcacatcc
     3121 ctctttatgt aaaaaattaa attgtgcttc tgtttctacc ccttctgcaa ttactttaaa
     3181 ttttaattgt tttgctagag caataatcgc tgtaataatt gcgccatcat ctaacgcttt
     3241 attggcatga ttaatatttc tgataaatga ttgatcaatt tttaaagaat caacaggaaa
     3301 tttttttaag ctgctcaaag aagaatatcc t
//
LOCUS       DAORVT010000126         3306 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_24684_length_3306_cov_3.282067, whole genome shotgun sequence.
ACCESSION   DAORVT010000126 DAORVT010000000
VERSION     DAORVT010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24684_length_3306_cov_3.282067"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="PK389_03285"
     CDS             <1..914
                     /locus_tag="PK389_03285"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HQY22766.1"
                     /translation="DDELDHPLGARFLAVEAGIDVVFPVLHGPMGEDGTVQGLLELAG
                     VPYVGSGVLGSAVGMDKDVMKRLLRDAGIPVAQFLTLQEHSYKSVSFEHIQTTVGLPF
                     FVKPANAGSSIGISKVKNRSEFEEKIALAFQFDRKILIEECITGREIECSVIGNETPL
                     TSLPGEIISAHEFHSYEEKYLGHGAIFKYPAYLKPNEISALQALAVKTYQALCCEGMG
                     RVDMFLKPDGQIIVNEINTIPGFTSTSAYPKMWEASGLGLSDLIDQLIVLALERFKKE
                     QGLKTTFKSVEADLCAAPKMQPVVSDL"
     gene            complement(883..1878)
                     /locus_tag="PK389_03290"
     CDS             complement(883..1878)
                     /locus_tag="PK389_03290"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HQY22767.1"
                     /translation="MNPIEHAIALLKAGKLVAIPTETVYGLGADARNPIAIQNIYLAK
                     ERPSSNPLIIHISDMSALSDWAIDIPDTAFQLAKAFWPGPLTLILARHPSVPKIVTGG
                     QESIAIRIPNHLLTLELLERFDGGIAAPSANRYGRISPTTTEHVRTELGSRVDYILEG
                     GACTLGIESTIVSLLDDIPIILRQGSISETDLSAVLKQPVRQKQSEKGAVKLEHDLIT
                     PGSHLSHYAPTTPLYLCKKERFLETALNWARNNKRFCALSFSVPVNALLEQGVPDYCI
                     AKWIVASENHHMYAQKLYAYLHDFDATKSDCLLIENPPNTAAWAAIRDRLQRAAS"
     gene            2012..2434
                     /locus_tag="PK389_03295"
     CDS             2012..2434
                     /locus_tag="PK389_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HQY22768.1"
                     /translation="MRRLALPLIITAFGTFFVARVFADLSGSLDEKAIIQRIHPEGEV
                     TIEAGSAMVVETPKVILDVGQQHYEQVCHICHGTGLAGAPKFGDKADWAPRIADGMAT
                     MVKRAIEGYKAMPPKGTCTNCSDDDIKKTVEYMVSHSQ"
     gene            complement(2446..>3306)
                     /locus_tag="PK389_03300"
     CDS             complement(2446..>3306)
                     /locus_tag="PK389_03300"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY22769.1"
                     /translation="FAAPRDAFVAEKTKNKKGMALGLLTVSKTAGCVLGPLLVSASTL
                     FLGTLKENLNILVVFCCLLVFPVFLMSFTLSAKGIETSEFSMREFRSVFKKILPILFL
                     ALLFFMGRFNDGILMIYLKHNGFPEWFYLSTIAIFNFIMLISSPFMGHHIDRGHVKKV
                     LYITIGALCAFNICFFHINSMNWVLAILGIISWGIQRTGAQIVFSSLVFQGVAKANYG
                     TAIGLFYIVSGFGTMVASFICGYMAQDSFSSIFIFSGLFGFASLGLSFSLLNRKTSSI
                     DLPGNAQQPA"
BASE COUNT          987 a          657 c          777 g          885 t
ORIGIN      
        1 cggacgatga actggaccat ccgcttggcg ctaggttcct cgccgtagag gcggggatcg
       61 acgtcgtttt ccctgtgctc cacgggccga tgggcgaaga cggcaccgtg caagggctgc
      121 tcgagctcgc cggcgtgccg tacgtgggca gcggcgtctt gggctccgcg gtcgggatgg
      181 ataaagacgt gatgaagcga ttgttaagag atgcgggtat tcccgttgcg caatttttaa
      241 cgttgcagga acattcctat aaaagtgtca gtttcgaaca cattcaaaca actgttggat
      301 tacctttctt tgttaaaccg gcgaatgccg gatcttctat tggcatttca aaagtaaaaa
      361 acagatctga atttgaagaa aaaattgcgc tagctttcca gtttgatcga aaaatcttaa
      421 ttgaagaatg tataacaggt cgtgaaattg aatgttctgt cattggaaat gaaacgcccc
      481 tgacttctct gccaggtgag attatttctg cacatgaatt tcattcatac gaagaaaaat
      541 atttagggca tggggcgata tttaaatatc ctgcgtattt gaagccaaat gagatttcag
      601 cgctacaagc gcttgcggtt aaaacatatc aggcgctatg ttgcgaaggc atggggcgcg
      661 tcgatatgtt tttaaaaccc gatggtcaga ttattgtgaa tgaaattaac acgattcctg
      721 gttttacctc aaccagcgcg tatcccaaaa tgtgggaggc aagcggtctt ggtttgtcgg
      781 atcttatcga tcaattaatt gttttggcgc tcgaacgctt taaaaaagaa caaggcttaa
      841 aaacaacttt taaatctgta gaggcagatc tctgtgctgc tcctaagatg cagcccgttg
      901 taagcgatct ctaatagccg cccatgctgc tgtgtttggt ggattttcga ttagaaggca
      961 gtcacttttt gttgcatcaa aatcatgtaa ataagcgtaa agcttttggg catacatgtg
     1021 gtgattctct gaagccacta tccattttgc aatgcaataa tctggaacac cctgttcaag
     1081 gagtgcattt acgggcacgg agaaactgag cgcacaaaat cgtttattgt ttcgagccca
     1141 gtttaacgcc gtttcgagaa atctctcttt tttacataaa tacagaggtg ttgtgggtgc
     1201 gtaatgagag aggtgtgacc caggcgttat taaatcatgt tccaatttta cagcgccttt
     1261 ttccgattgt ttctggcgca caggttgctt taatacggca gataaatcgg tttcagaaat
     1321 gctaccttga cgcaagataa tggggatgtc gtctaataga gaaacaatcg tcgattctat
     1381 acccagcgtg caagccccgc cttctaaaat ataatccacg cgtgatccta gttcggttcg
     1441 aacatgttca gtggtggttg ggctaatacg accgtatcga ttcgccgagg gtgcggcaat
     1501 accgccatca aatcgttcta acagctcgag tgttaaaaga tgattgggaa tacggatcgc
     1561 aatgctctct tgtccacccg tgacgatttt gggaacacta gggtgccgtg ccaatatcaa
     1621 cgtcagcggg cctggccaaa atgcctttgc tagctgaaaa gcggtgtcgg ggatatcgat
     1681 ggcccaatcg ctcagtgcgc tcatgtcaga tatgtgtatt atcaacgggt tgctagaagg
     1741 ccgctcctta gccaggtata tattttgaat ggcaatgggg tttctggcat ctgcccccaa
     1801 gccataaacc gtttccgttg gaatggcgac taattttcct gcttttagca aagctatcgc
     1861 gtgttcaatg ggattcatgc gtgtctcaat agattaagtt gttaggataa ttttggaatg
     1921 cacgcaaggg aagaccctgt gcgaaaaagg ctataatatg ataaattagc acattcgtct
     1981 aggtgttgtg ttgtagggag aaaaaagaac tgtgaggcgc ttagcattgc cactcattat
     2041 taccgcgttt ggtacttttt ttgttgctcg agtatttgcg gatttatcgg gttcgctcga
     2101 cgaaaaagca attatacagc ggattcaccc agagggggag gtaacgattg aggccggtag
     2161 cgcaatggtt gtagaaacac ctaaggttat attggatgtt ggtcaacaac attatgagca
     2221 agtgtgtcat atttgtcatg gcactggtct tgcaggtgca ccaaaatttg gcgataaagc
     2281 agattgggca ccgcgtatcg cagatggtat ggcgacaatg gtgaagcgtg caatagaagg
     2341 ttacaaggcg atgccaccga aaggaacctg tacgaattgt tctgatgatg acatcaaaaa
     2401 aacggttgaa tatatggtgt cgcattctca gtaaggcata aacaactaag caggctgttg
     2461 cgcgtttccc ggcaaatcaa tggaggaagt tttcctgttt aaaagactga aggataagcc
     2521 taaagacgca aagccaaaga gtccactaaa aataaatatt gaagaaaaag aatcttgagc
     2581 catgtatcca cagataaatg aagctaccat ggttccaaaa ccacttacga tataaaataa
     2641 accaattgct gtaccgtaat tggcttttgc aacgccttga aacaccagcg aagaaaaaac
     2701 aatttgtgcg ccagtacgtt gtatacccca agaaataatg ccaaggatag ctaaaaccca
     2761 attcatggaa ttaatgtgaa agaaacaaat attaaaagca cataaagcgc caatcgtaat
     2821 atagagtact ttttttacat gtccacgatc aatgtgatgt cccataaagg gagagctgat
     2881 gagcataata aagttgaaaa tggcaatggt cgaaagataa aaccattccg gaaatccgtt
     2941 gtgtttaaga taaatcatca agatgccgtc attaaagcga cccataaaaa ataataaagc
     3001 taaaaaaaga atcggtaaaa tctttttgaa gacagaccta aattctcgca tcgaaaattc
     3061 actggtttcg attccttttg cactgagtgt gaacgacatc aaaaaaactg gaaaaacgag
     3121 taaacaacaa aaaacaacca gaatatttaa attctctttc aaggtgccca aaaagagtgt
     3181 agaagcactc accaacaaag gaccaagtac acatcctgcg gttttagaaa cggttagcaa
     3241 ccctaaggcc atgccttttt tgtttttagt tttttcagca acgaaggcat cacggggtgc
     3301 cgcaaa
//
LOCUS       DAORVT010000127         3299 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_24780_length_3299_cov_3.262947, whole genome shotgun sequence.
ACCESSION   DAORVT010000127 DAORVT010000000
VERSION     DAORVT010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24780_length_3299_cov_3.262947"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1425
                     /gene="mnmG"
                     /locus_tag="PK389_03305"
     CDS             <1..1425
                     /gene="mnmG"
                     /locus_tag="PK389_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008950601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HQY22770.1"
                     /translation="KGVVTQCGICFFAKAVVLTTGTFLGGKIHIGTRQHAAGRAGDPP
                     SIALADRLRELHPRVGRLKTGTPPRIDGRSIDFSILEEQKGTEPTPTFSFFGKVADHP
                     KQVSCFITHTNQQTHDIIQQDLKYSPLFTGAIEGIGPRYCPSIEDKVVRFSDRAAHQI
                     FIEPEGLETYEVYPNGISTSLPFETQFKLVRSIRGLENAHITRAGYAIEYDYFDPRDL
                     KATLETRPLNRLFFAGQINGTTGYEEAGAQGLLAGINAACMALDKEPWIPKRSESYLG
                     VLVDDLITLGTKEPYRMFTSRAEYRLILREDNADLRLTTLAHQLGILGETQWRAFQEK
                     QEAILQETERLRGLRAFDLLRRPEVSYKDLVARPEFGPGVLDPLVAEQIEVQIKYEGY
                     INAQTREIARQEKYGVLHLPKNMDYSKITGLSTEILEKLTSVQPETIGHAARIPGVTP
                     AAISLLLVYLKKRGHLRDKESDFN"
     gene            1400..2086
                     /gene="rsmG"
                     /locus_tag="PK389_03310"
     CDS             1400..2086
                     /gene="rsmG"
                     /locus_tag="PK389_03310"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HQY22771.1"
                     /translation="MIKKAILIKALSEGLSAANLSILESTQLQFVEYIELLVKWNAVH
                     NLTAIRDPLEMVKRHVLDSLVLLPYFEAFCKNRPEGRVSVLDVGTGAGLPGILLALCR
                     PEQSFVLLDSNQKKIAFVQHVILSLKVSNVIAVCDRVEKYRPEVLFDWIVSRAFASLS
                     DFAHLSQHLCKPDGQLVAMKGIIDQVEVEKVSSDYRIEKMASVQIPGIEAERTLVFMS
                     ITQKGDFGAK"
     gene            2076..2903
                     /locus_tag="PK389_03315"
     CDS             2076..2903
                     /locus_tag="PK389_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HQY22772.1"
                     /translation="MPNRVVAFANQKGGVGKTTSAVNLAAALAAHQRDAHNKYKVLLI
                     DLDPQGNATMGSGAIKNQLKYTVNEVLQEEVTIQNAIIRKTPSGYALLAANADLTAAE
                     VALLMKPYQEMILKNAINSVRAEFDWVIIDCPPSLNMLTLNALVAADSVIIPMQCEYY
                     ALEGLTALMSTIEQIRSGLNPALEIEGILRTMYDPRNRLSTEISAQLVAHFGNKVYQT
                     VIPRNIRLAEAPSHGVSIISYDERSRGSEAYMALAREMLKQYQPNHSNQATAQAVVV"
     gene            2915..>3299
                     /locus_tag="PK389_03320"
     CDS             2915..>3299
                     /locus_tag="PK389_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HQY22773.1"
                     /translation="MSQNRLGKGLEALLGAARAIPLPERDPAKTVHHGVLELAVKQLA
                     PGRYQPRRIMREAELETLADSIRSQGIIQPILVRKTGEDHYEIIAGERRWRAAQMAGL
                     AKVPVVVKEVSDENAMAIALIENIQR"
BASE COUNT          952 a          642 c          791 g          914 t
ORIGIN      
        1 aaaggggttg taacacagtg cggcatttgt ttttttgcga aagcagttgt gttaacgacc
       61 ggtacctttt tgggcggaaa aattcatatt ggtactcggc aacatgctgc cggacgtgca
      121 ggcgaccccc cttctattgc attggccgat cgattacgcg aattacatcc acgtgttggg
      181 agattaaaaa caggaactcc accacgcata gatggcagga gtattgattt ttctattcta
      241 gaagaacaaa aaggaacaga acctacaccg acattttctt tttttggaaa agtagccgat
      301 catcctaaac aggtttcttg ttttattaca cataccaatc aacaaacgca tgatattatc
      361 cagcaagatc tcaaatattc gccgctattt acaggtgcta ttgagggtat aggtccacgt
      421 tattgtcctt ctattgaaga taaggtggtt cgctttagcg atcgagctgc gcatcaaatt
      481 tttatagaac ccgagggttt agaaacctat gaagtttatc ccaatggtat ttcgaccagt
      541 ttgccttttg aaacacaatt taaactcgtg cgttctattc ggggtttaga aaatgctcac
      601 attacacgtg cgggatacgc gattgaatat gattattttg atcctcgtga tcttaaggca
      661 acgctcgaaa cacgaccgct gaacaggctc ttttttgcag ggcaaattaa cggcacaacc
      721 ggttatgaag aagcgggtgc ccaaggatta ttagcaggca ttaatgccgc gtgtatggcg
      781 cttgataaag aaccttggat acctaagcgc agcgagtcgt atttaggcgt cttggtcgat
      841 gatttaatta cgctaggcac aaaagagcca tatcgtatgt ttaccagccg cgccgaatat
      901 cgtttaatct taagagaaga taatgcagat ctgcgtctaa caacccttgc acaccagtta
      961 ggaatactgg gtgaaacgca atggcgtgct tttcaagaaa aacaagaagc aattttgcaa
     1021 gaaacagagc gtttgcgtgg attaagagcc tttgatctat tgcgacgccc cgaggtttcg
     1081 tataaagact tggttgcacg tccagaattt ggcccagggg tattagatcc gcttgttgca
     1141 gaacaaattg aagtgcaaat taaatacgaa gggtatatta atgctcaaac gcgagaaatt
     1201 gcgcgacaag aaaagtatgg ggttttgcat ttacccaaaa atatggatta ttcaaaaatc
     1261 actggactat cgacagaaat tttagaaaaa ttaacaagtg ttcagcccga aaccatcggg
     1321 catgcagccc gtatcccggg tgttacaccg gcggcgattt ctttgttatt agtgtattta
     1381 aaaaagcgag gtcatttgcg tgataaagaa agcgatttta attaaagcgc tttcagaagg
     1441 gctttctgct gcaaatctca gtattctaga atcaacgcaa cttcaattcg ttgagtatat
     1501 tgaattactg gttaaatgga atgcggtgca taatctgacc gccattcgcg acccactcga
     1561 gatggttaag cgacatgtgt tagatagctt ggttttattg ccttattttg aggctttttg
     1621 taaaaataga ccagaaggac gcgtttctgt gttggatgtc ggcacaggtg cgggtttgcc
     1681 tggaatcctg ctggcgctct gtagacctga acagtccttc gtgctactag acagcaatca
     1741 aaagaaaatt gcctttgttc aacatgtgat attatcttta aaagtgagca atgttatcgc
     1801 ggtatgcgat cgtgtagaaa agtatcggcc agaagtcttg tttgattgga ttgtctcgcg
     1861 ggcatttgcc tctttatctg actttgcgca tttgtctcag catctgtgta aacccgatgg
     1921 tcaattggtt gcaatgaagg gtattatcga tcaagtagag gttgaaaagg tctcatcaga
     1981 ttatagaata gaaaaaatgg cgtctgtgca aattcctggg atagaagcag aacgcacctt
     2041 ggtttttatg agtatcacac aaaaaggaga ctttggtgcc aaatagagtg gtagcctttg
     2101 caaatcaaaa aggcggagtt ggtaaaacga ccagcgctgt taatctggcg gcggctttgg
     2161 cggcacatca gcgtgatgca cataataaat ataaagtttt attaattgat ttagatcctc
     2221 aagggaatgc cactatgggt agtggcgcga ttaaaaatca gctgaaatat acggttaatg
     2281 aagttttaca agaagaggtc actattcaga atgcgataat acgtaaaacg ccctcaggtt
     2341 acgcattact cgctgcaaac gccgatctta ccgccgcaga agtagcttta ttaatgaaac
     2401 cgtatcaaga aatgatttta aaaaacgcaa tcaattctgt tcgagcagaa tttgattggg
     2461 tgatcattga ttgcccgcct tcgttgaata tgcttacctt aaatgccctt gtggcggcgg
     2521 attctgtcat tattccgatg caatgcgaat attatgcgtt agaaggctta acggctttga
     2581 tgagcactat cgaacaaatt agaagtggat taaatcccgc cttggaaatc gaaggcattt
     2641 taagaaccat gtatgatcca cgtaatcgat tgtctaccga gatctctgcg caattggttg
     2701 cgcattttgg taataaagtt tatcaaacgg tcatcccgcg gaatattcga ttagcagaag
     2761 cgccgagtca tggcgtatcg attatatcat atgatgagcg ttctcgtggc tcagaggcgt
     2821 atatggcgct tgccagggag atgttaaaac aatatcaacc caatcattca aatcaagcaa
     2881 cagcacaggc tgttgtggtt taggggggaa tacgatgtcg caaaatcgat taggaaaagg
     2941 gctagaagca ttgttgggcg ctgcgcgtgc gatcccctta ccggaaagag accctgcaaa
     3001 aacggtacat cacggtgttt tggagctcgc tgttaaacag ttagcgccgg ggcgatatca
     3061 gccgcgacgc attatgcgcg aagcagagtt ggaaacgctc gcggattcta ttcgatcgca
     3121 agggattatt cagcctattt tggttcgaaa aacgggtgaa gatcattacg aaattattgc
     3181 gggtgaacgc cgttggcgtg cggcgcaaat ggccggctta gccaaagtgc cggtggttgt
     3241 caaagaagtt tccgatgaaa atgcgatggc tattgcgctc atcgaaaata ttcaacgtg
//
LOCUS       DAORVT010000128         3278 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_25054_length_3278_cov_2.751784, whole genome shotgun sequence.
ACCESSION   DAORVT010000128 DAORVT010000000
VERSION     DAORVT010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25054_length_3278_cov_2.751784"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PK389_03325"
     CDS             <1..196
                     /locus_tag="PK389_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HQY22774.1"
                     /translation="VLEYAYLKGYTQQEISDTLEMPLGTVKTRLRAAIGMLREELKGE
                     KNYFLGGPGLRNLLLLLKWI"
     gene            235..972
                     /locus_tag="PK389_03330"
     CDS             235..972
                     /locus_tag="PK389_03330"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HQY22775.1"
                     /translation="MDQLTPAEKAEVEGYLASYPELLTDVYEIGRSLELVAMSAARPA
                     PSGLKDKILNDIRNSAPKDSPQTNTTVQGGFGALHAIAALLGLGVLLFGYMFWQKNTA
                     LNTLQGEYTVLRDSCAQNTAELTSQLEIFRQLTFPDNKIIPFQATPGFASTDLYLHYN
                     AVTKRNFIQVRNLPSLAANQSFQLWSLKPDQAPAPLTVFEIPANGLIEVAFEAGTDTY
                     AITIEPKGGRETPTLENLIGTVSVAGI"
     gene            1211..2785
                     /locus_tag="PK389_03335"
     CDS             1211..2785
                     /locus_tag="PK389_03335"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY22776.1"
                     /translation="MYRILYSFGLLLNLYVVHGQTTFSVLPDVGGINRQAMGILSVPE
                     PEDIIIMGHRFDTILPGSNAKPYLAHFSYDGELKFVVPIVDSLYEGPFSLAYPIPPYQ
                     KNDSIYYFEAIRDIGDPFLRPYLTELNINTGKILRSIVLLNLQHPLLPNEPHDLNYTV
                     NNEILLLSSLINGDSVQTYITLLDSAFNEVRLIKVKDVSKKTFASICNKNLNGTFDIV
                     GYCLVEQELGNDYDSFYLDIVDGNGNVLEHTLAPTLIPISRGVYFSRTILQDKSNNWI
                     IGGTFRQDLPDICSDCYELIPYIFSTTPDFKTLRWQTKFFDVPYYGAPQYFLHSMTQV
                     EDGFIVSGEFYNYNGFDNGVLFKASDNGDSLWMKHYIPLNWNEDRVGSVRFTDIQTSP
                     YGTIIVTGAVGDFQENIIRPWILQLDKDGCLVPDCNAVGTHHEDVESSQENFTIFPNP
                     ASTEIYVLSHISSAASLTISIFSIQGIEIKKRSFDPRSGYQYALPVNDLDEGLYFLVV
                     TDPKSGHRESHQFIRK"
     gene            2875..>3278
                     /locus_tag="PK389_03340"
     CDS             2875..>3278
                     /locus_tag="PK389_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY22777.1"
                     /translation="MEIKLATDQDVDKLIPVMIELRPHRSPAELREMILAQMKNGYVV
                     VYAGDETQAFAVGGFRTIDFLFSGKTLYVDDLVTHSQHRKKGYAGMLMKWMIQYAKEN
                     NYDHFSLDSGHQRKDAHRLYLNHGLDITAHHFG"
BASE COUNT          961 a          627 c          726 g          964 t
ORIGIN      
        1 cgtgctggaa tatgcctatt tgaagggcta tacccagcag gagatctcag atacgcttga
       61 aatgccgctt gggacggtca agaccaggct cagggcggct attggcatgc tgagggaaga
      121 attaaaagga gaaaagaact atttcctggg tggaccaggt ttaagaaatc tattattgtt
      181 gctcaagtgg atatagcaga aatcaggtca tcgggtttat tggagctcta cgtcatggac
      241 cagctcaccc cagccgaaaa ggctgaggtg gaagggtatt tggcctccta tcctgagctc
      301 ctgactgacg tctatgagat cgggcgaagc cttgaactcg ttgctatgag tgcggccaga
      361 cctgcacctt ctggattgaa agacaaaatt ttaaatgata tcagaaacag tgctccaaaa
      421 gattcgccac agaccaacac aacagttcaa ggtggatttg gtgcattgca tgctattgct
      481 gcgcttctag gtctgggcgt ccttctgttt ggatacatgt tctggcaaaa aaatactgcc
      541 ttgaatacgt tacaaggtga gtacactgtc cttcgggatt cctgcgctca gaatactgca
      601 gagctgacat ctcaacttga aatattcagg caacttacat ttcctgataa caagatcata
      661 ccattccagg ctacaccagg atttgcctcc acagatcttt acctgcatta caatgcggtg
      721 acaaaacgca actttatcca ggtgcgtaac ctaccatcac tggctgcaaa tcagtcgttc
      781 caattgtggt ctttaaaacc tgatcaggca ccggcgccgc tcacagtttt tgaaatacca
      841 gctaatggtt tgatagaagt ggcatttgaa gcgggaacgg atacttacgc tattacgatt
      901 gagccaaagg gaggaagaga aacacctacg ctggagaacc tgatcgggac ggtgagtgtg
      961 gcggggattt gatgcaagga gcagtaggca gtaggcagta ggcagtcagc agttgtcagt
     1021 tggcagttga cagttggcgg gtagcagtca gcaatcagca gttggcagtt ggcgggtagc
     1081 agtagagata tacacttggc tccactcaaa catggcactc acgactcacg acattctttg
     1141 atccaatttc aaagatctct gaaactcata aaattgccct atattaattg tcaccttact
     1201 taggttgtcc atgtatcgaa tactttattc ttttggattg ttactgaatc tctatgtcgt
     1261 acatggacaa actactttta gtgtactgcc tgatgttggt ggaataaata ggcaggctat
     1321 gggaattttg agcgtaccag agcccgaaga cattattatt atgggtcatc gattcgatac
     1381 cattttacca gggtctaacg ctaaaccata cctagcacat ttttcttatg acggcgaatt
     1441 aaagttcgtt gtgccaattg ttgatagttt gtatgaggga cctttcagtt tggcttatcc
     1501 tattcctcca tatcagaaaa atgacagcat ttattatttt gaggccataa gggatatcgg
     1561 tgatcctttt ttgcgtccct atttaacaga gcttaatatt aatacgggaa agattttacg
     1621 ttcaattgtt ttgttgaact tacagcaccc tcttttgcca aatgagcctc atgatttaaa
     1681 ttatactgta aataacgaga tattactttt aagttcttta ataaatgggg attctgtaca
     1741 aacgtatatt accttgttag attctgcttt taacgaagta agattaataa aagtgaaaga
     1801 tgttagcaaa aaaacatttg cttcaatttg taataagaat ttaaatggaa cttttgatat
     1861 agttgggtat tgtctggttg aacaggaatt agggaatgat tatgatagct tttatttaga
     1921 tattgttgat ggaaatggaa atgtccttga gcatacttta gctccaacac ttatcccaat
     1981 ctctcgggga gtatactttt caagaactat tttacaagat aaaagtaata attggattat
     2041 tgggggcaca tttcgccaag atttaccaga catatgttct gattgttatg aattgatacc
     2101 ttatatattc agtactacac cagattttaa aacactacgc tggcaaacaa aattttttga
     2161 tgtgccctac tatggagccc ctcaatactt tttgcattcg atgacacaag ttgaagatgg
     2221 ctttattgtt tctggggaat tctacaatta taatggtttt gataatggtg tgctgttcaa
     2281 agcatctgat aatggggata gcctttggat gaagcactat attccactaa actggaatga
     2341 agatcgggtg ggatcagttc gatttaccga cattcaaact tctccgtatg gaactattat
     2401 tgtgacaggt gctgtagggg attttcagga gaatattatt cgcccatgga ttctacaatt
     2461 ggataaagat ggttgccttg tgccggactg caatgcggtc ggcacccatc atgaagatgt
     2521 cgagtcttcg caggagaatt ttaccatttt tccaaatcca gcatccacag agatatatgt
     2581 acttagtcat atttcttcgg cagcgtctct tacaatcagt attttttcaa ttcaaggtat
     2641 agaaatcaag aaaagaagtt ttgacccaag gtctggttat caatatgcgc tgccagttaa
     2701 tgacctcgat gagggtcttt attttttagt ggtaacagat ccaaagtcgg gacatagaga
     2761 aagtcatcaa tttattcgca aatgaaggtt tttcagtagg caaggagata tagaaaaaac
     2821 gactcacgat tcacgcctca cgacactcac cactcacaat tcacaataat taaaatggaa
     2881 atcaaactag ctactgatca ggacgtggac aaactgatac ctgtcatgat cgaactgagg
     2941 ccacatcgaa gcccggcaga gttgcgggag atgatcttag cgcagatgaa aaatggatat
     3001 gtggtcgtat atgccggtga tgaaacgcaa gcctttgctg taggcggctt caggactatt
     3061 gattttttat ttagtggcaa aaccttatac gtagatgacc ttgtgaccca ctcacaacac
     3121 aggaaaaagg gatatgccgg tatgctgatg aaatggatga ttcaatatgc caaagaaaat
     3181 aattacgatc atttttcttt ggactcagga catcagcgta aagatgcgca tcggctatac
     3241 ctcaatcatg gcctggatat cacggcacat cattttgg
//
LOCUS       DAORVT010000129         3256 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_25367_length_3256_cov_3.226179, whole genome shotgun sequence.
ACCESSION   DAORVT010000129 DAORVT010000000
VERSION     DAORVT010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25367_length_3256_cov_3.226179"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="PK389_03345"
     CDS             complement(<1..1051)
                     /locus_tag="PK389_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22778.1"
                     /translation="MQLHLRDVFMMTLEKAQEQVRLLTGSLEQAHSYEAVLKLKNDIA
                     QLITGFNNLDKSKTFASPVELSALRTGIASLRSAYQASIRSFPKLALQQSLKANTSRA
                     VEAETLGESKVSYEKTQTSNLLKEGLTLLSYNIDTNILRTEEGYARDAFPEWRANERA
                     SSIREAMGKIIHSYRPTIIQIQEGRKFTTKFGDEVDSVTPLVNYLKEAGYNVLVQPYN
                     GGGGEKAFKFITAYDPRALTLLENGTYIRYLTQTPEQPTPRPSIEGKSKEEVEKNISS
                     IKSHNFGVEWERGVFIVGLQEPSTGQKIYSLNAHFDIVLEARKNSSRLLVEFVKEIIV
                     KEPDAKILISGDFNTF"
     gene            complement(1185..1260)
                     /locus_tag="PK389_03350"
     tRNA            complement(1185..1260)
                     /locus_tag="PK389_03350"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1225..1227),aa:Lys,seq:ctt)
     gene            complement(1478..1795)
                     /locus_tag="PK389_03355"
     CDS             complement(1478..1795)
                     /locus_tag="PK389_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22779.1"
                     /translation="MIAFLMDEHGAWRVSPAYDLTFSFGPNGEHSTMIMGEGKNPTLN
                     HLLRLAAIGEIKKPKALEIIEHVHSATNKWKDCAESTGVSKPQIKRIGETLASVKNIF
                     TNA"
     gene            complement(1773..2015)
                     /locus_tag="PK389_03360"
     CDS             complement(1773..2015)
                     /locus_tag="PK389_03360"
                     /inference="COORDINATES: protein motif:HMM:NF025041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HQY22780.1"
                     /translation="MTFTPQNLIYVYFNSLKNKHLMGRLLLKNRKLFFEYDATFLKLG
                     LELSPFKLPLKSGVISNDDRTFDGLFGVFNDSLPDG"
     gene            complement(2012..2317)
                     /locus_tag="PK389_03365"
     CDS             complement(2012..2317)
                     /locus_tag="PK389_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQY22781.1"
                     /translation="MMSVLMTTPHEMTEHLAKKARAKRLSMNLSQQTLAEQSGISYSV
                     LKKFERTGKISLASLLKLALSLDALEDFYGLFDLKKPEDAASLDELFEDKTRKRGRK"
     gene            2425..2526
                     /locus_tag="PK389_03370"
                     /pseudo
     CDS             2425..2526
                     /locus_tag="PK389_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019349587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(2582..2657)
                     /locus_tag="PK389_03375"
     tRNA            complement(2582..2657)
                     /locus_tag="PK389_03375"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2622..2624),aa:Thr,seq:tgt)
     gene            complement(2751..2865)
                     /gene="rrf"
                     /locus_tag="PK389_03380"
     rRNA            complement(2751..2865)
                     /gene="rrf"
                     /locus_tag="PK389_03380"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(2918..>3256)
                     /locus_tag="PK389_03385"
     rRNA            complement(2918..>3256)
                     /locus_tag="PK389_03385"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          907 a          700 c          619 g         1030 t
ORIGIN      
        1 cgaatgtatt aaagtcgccc gaaattaata tttttgcatc gggttcctta acaataattt
       61 ctttgacgaa ctcgactaat aacctagaag agtttttccg agcctctaaa acaatatcga
      121 agtgcgcatt aagagagtat attttctgcc cagttgatgg ctcttgtaag ccaacaatga
      181 atacacccct ttcccattca accccaaaat tgtgactctt aatcgatgag atattttttt
      241 caacttcttc tttacttttt ccttcaatac ttgggcgcgg agtaggctgc tcgggtgttt
      301 gggtcaaata tcggatgtat gtaccatttt cgagcaaagt taacgctctc ggatcatagg
      361 cagtaatgaa tttaaaagcc ttttctccgc ccccaccatt atagggttgc actaagacat
      421 tataaccagc ttctttaaga tagttaacaa gaggggttac tgaatcaact tcgtcaccga
      481 atttagttgt aaattttctg ccctcttgga tttgtataat tgtagggcga taggaatgaa
      541 ttattttacc catagcttct ctaatggaag aagctctctc atttgcgcgc cattcaggaa
      601 aagcatctct tgcataccct tcctctgttc ttaatatatt agtatcgata ttgtaactca
      661 ataaagttaa gccttctttt aataaattac ttgtttgggt cttttcatat gatactttac
      721 tttcgcctaa tgtttctgcc tcaactgcac gactagtatt ggcttttaag ctctgctgca
      781 atgcaagttt aggaaagctt ctaatacttg cttgataggc gcttcttaag ctggcgatcc
      841 ctgttcgtaa agcgcttaac tctactggag atgcaaaggt tttagattta tcaaggttat
      901 taaagccggt gattaattgt gcaatatcat tttttaactt taaaaccgct tcataactat
      961 gagcctgctc caaagaacct gttaataatc tcacttgttc ctgagccttt tcgagcgtca
     1021 tcataaaaac atctctcaaa tgtaattgca ctatcgcgcc caaattctac ttaattaggt
     1081 aactaatcgt caagcttttc tgtgtatata agggggttca ccgaatgttt accgcaagcg
     1141 gttttttcag ggcacgggga gctaacataa gtgcttgatt ttaatggtgg gccgtgttgg
     1201 gatcgaacca acgacccgct gattaagagt cagctgctct accaactgag ctaacggccc
     1261 aaactagttg tgaatgatac tccttcacgt aaccagaaac aattggtaaa ttctttttta
     1321 agtttttgat ttattttgac caacatcact catgacttaa tgatgcaatt ttatcggagt
     1381 gtcagaatat caatggctcc aaggcaaaag catcgacctt attcagttgc tttttctaca
     1441 catgaccgcc ataagatttt cccttagtat ctctttctta tgcatttgtg aaaatgtttt
     1501 taacagaagc taaagtctcg ccgattcttt ttatctgcgg ttttgagacc ccagtgcttt
     1561 ctgcacagtc tttccatttg ttggttgcag aatgcacgtg ttcaatgatt tctagtgcct
     1621 ttggcttttt gatctctcct atagcagcaa gccttaataa atgattaaga gtaggatttt
     1681 taccctcgcc cataatcatc gttgaatgct cgccattggg gccaaaagaa aacgtaagat
     1741 catatgcagg cgatacacgc catgcaccat gctcatccat caggaaggct atcattaaaa
     1801 acgccaaata acccatcaaa agttcgatcg tcgttgctta taacaccact tttaagtggt
     1861 aatttgaaag gagataactc aagaccaagc tttaaaaaag tcgcatcata ctcaaaaaat
     1921 agctttctat ttttgagtaa taaccgaccc atcaagtgct tgtttttaag actattaaaa
     1981 tatacataaa taagattttg tggcgtaaat gtcattttct acctcttttt cgtgttttat
     2041 cttcgaacaa ttcatctaaa gaggcagcat cttcaggttt ttttagatca aataaaccat
     2101 aaaaatcttc gagtgcatct aaactcaggg ctagctttaa taaagaagca agcgagattt
     2161 ttcctgtccg ttcaaatttt tttaaaacac tgtaactaat acccgattgc tccgcaagtg
     2221 tttgttggct aaggttcatg gagagtcttt tagcccttgc ttttttggct aaatgttctg
     2281 tcatttcatg gggtgtagtc atcaatactg acatcataat gtctgttata ccgatttaat
     2341 agtcacagta gacattataa tatacataat ccagcgagtc aaacggtgac agtgggaaaa
     2401 ggagaacctt tttaggcggc ctctggcagg cgtttacgat cagctggagt ttcatttgag
     2461 gatagacagg atttactggg tcataagtca cataggatta cgacgcatta ttcttctgct
     2521 gaattgttga aaaggagttt ttggaaagtt tactgaaaaa cgtgtaagtg tttgatttaa
     2581 atggagctgg tgactggatt cgaacccgcg acctgctgat tacaaatcag ctgctctacc
     2641 aactgagcta caccagcaga attgtgtcga ttgtaacgga ttgcgtaacg atacgcaatt
     2701 tttgcgcaat aagaaaggct ttatgttaat aaagcctttt cgtatatggc gcctggcgat
     2761 gtctactttc acatggggaa accccacact atcattggcg ctgagcggtt tcacttccga
     2821 gttcgagatg gattcgggtg ggtcacactc gctattatca ccaagcataa aacgtcatta
     2881 agaaggtact tctcacaaaa aatcatttaa gcattatatg gccaagccgc acggccaatt
     2941 agtacaggtt agctcaacgc ctctcagcgc ttacacaccc tgcctatcta cgtcgtcgtc
     3001 ttcaacgggc cttcagaaga cttatagtct tgggagatct tatcttgagg caggcttccc
     3061 gcttagatgc tttcagcggt tatcccttcc acacttagct acccggcaat gccactggcg
     3121 tgacaaccgg tacaccagag gtgtgtccat tccggtcctc tcgtactagg aacagaccct
     3181 ctcaaatctc caacgcccac agcagatagg gaccgaactg tctcacgacg ttctaaaccc
     3241 agctcgcgta ccactt
//
LOCUS       DAORVT010000130         3217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_25916_length_3217_cov_3.534788, whole genome shotgun sequence.
ACCESSION   DAORVT010000130 DAORVT010000000
VERSION     DAORVT010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25916_length_3217_cov_3.534788"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PK389_03390"
     CDS             <1..194
                     /locus_tag="PK389_03390"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HQY22782.1"
                     /translation="KNVYPDVTLENLLTEIRERDVRDRGRVTSPLIPAKGAVVLDTTG
                     LGIKEVFDLVLKEVNRRLY"
     gene            281..1960
                     /gene="rpsA"
                     /locus_tag="PK389_03395"
     CDS             281..1960
                     /gene="rpsA"
                     /locus_tag="PK389_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HQY22783.1"
                     /translation="MSENFAELFKQSIAKTNVHLGAVVTGTVIRISDEFVTVHAGLKS
                     EGHIPIDQFYDDNRQLEVKVGDQVEVVLDAVEDGWGETRLSREKAKRVECWTRLARAQ
                     EAGEAVKGIITGKVKGGFTVEIQTIRAFLPGSLVDVRPVRDTNTLEGKELEFKVIKLD
                     QKRNNVVVSRRSVVESEGSAERDELMTTLQEGQETKGVVKNLTDYGAFVDLGGIDGLL
                     HITDMAWRRVKHPSEIVSVGQELAVKVLKFDRERNRVSLGLKQLGEDPWVDITRRYPV
                     DTRLTGKVTNITDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVQLGQEAEVIVLD
                     IDEDRRRISLGLKQCKANPWEAFASAHTKSDKVSGKIKSITDFGIFIGLDGGIDGLVH
                     LSDIAWNESGEEAVRRYKKGEEIEAVVLAVDPERERISLGIKQLEGDPYASYTGEHAK
                     GSIVTGTILSVDAKGATIQLAEGVEGYLKASELGRDRVEDARTVLKEGDAIEAKFTGV
                     DRKTRQITLSIKAKDSEEEQEAMRELTRQEGASPTLGDLFKEKMESKDKRE"
     gene            1969..2205
                     /locus_tag="PK389_03400"
     CDS             1969..2205
                     /locus_tag="PK389_03400"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HQY22784.1"
                     /translation="MRLLSIITLTFAVLLGVSFAILNAGFVTVHYYVGTKSLPLSLLI
                     LGAWIFGIIVGLLISAFKIIGLRMELQRQHRVPR"
     gene            2209..>3217
                     /locus_tag="PK389_03405"
     CDS             2209..>3217
                     /locus_tag="PK389_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HQY22785.1"
                     /translation="MWEFLFLLLPLAALSGWLIGRRASMRQAIRRLAGLNKLLDHSSN
                     KKAVDVLVQLMDSNQEAVETHLALGGLFRRQGEVDKAIGIHERLIAKPSLTEEQRALS
                     LLELSRDYMRAGVLDRAESVLLELIELGYELDAGFQGLLDVYQQSKDWLRAIQTAQQW
                     QTKKNRNMSYHIAHFYCELAEQQWLKGERVDAYQHLKEALVTNKRCVRASLLLGNIEA
                     QAGRYRVAIRAYKQIQKQDEAFLSEIVLPLARCYESLHNAEGMIKYFDENLQKHLTTS
                     AVLAFAEWLNKSKGESEAMRFVVQHLRKNPSLRGLSRLVQFSLARAEGSLQQDLLLFQ
                     EL"
BASE COUNT          966 a          540 c          848 g          863 t
ORIGIN      
        1 tgaagaatgt ttatccggat gttacgctag agaatctttt gacggaaatc agagagagag
       61 acgttcgaga cagggggcgt gtaacctcac cgttaattcc ggcaaagggt gctgttgtgc
      121 tagacacgac agggttgggg ataaaggaag tgtttgatct cgtactaaaa gaggtcaatc
      181 ggcgacttta ttaattttta gttttaggcc tgggtcgcgt tatcctacta ttattaaata
      241 gcagagacga acacagagac agagattgga aaatattgat atgagtgaaa atttcgcaga
      301 attatttaaa caaagcatag ccaaaaccaa tgtgcattta ggtgcagttg taacggggac
      361 tgtgattcgt attagcgacg aatttgtgac tgtgcatgcc ggactaaaat cagaaggcca
      421 tattcccatt gatcaatttt acgatgataa tcgtcaatta gaagttaaag tcggcgatca
      481 agtcgaagtc gttttggatg cggtagaaga tggctggggc gaaacacgat tatccagaga
      541 aaaagcaaag cgtgttgagt gttggacacg tttagcaaga gcacaggaag caggtgaagc
      601 cgttaagggt atcattacag gtaaagtaaa aggcgggttt acagttgaga tccaaaccat
      661 tcgggccttt ttgcccggtt ctttagtcga tgtgcggccg gtacgtgata ccaatacgct
      721 ggaaggaaaa gaattagaat ttaaggtgat taaacttgat caaaaacgga acaatgttgt
      781 cgtatcacga cgttctgttg tggaatccga aggcagtgcc gagcgcgatg agttaatgac
      841 cacgctacaa gaaggccaag aaacaaaagg tgttgttaag aatttaaccg attacggcgc
      901 ttttgtggat ttgggcggaa ttgatggctt gttgcatatt actgatatgg catggcgccg
      961 cgtgaagcat ccaagcgaaa ttgtatccgt tggacaagag cttgcggtaa aagtcttgaa
     1021 attcgatcga gaaagaaatc gtgtatccct gggattaaag caattaggcg aagatccgtg
     1081 ggtcgatatt acacgtcgtt acccagtaga tacccgttta actggcaaag taacgaatat
     1141 aaccgattac ggttgttttg tggaaatcga agaaggtgtc gaagggttag tgcacgtttc
     1201 tgagatggat tggactaata aaaatataca tccatccaaa gttgttcaat tgggacaaga
     1261 agcggaagta attgttttgg atatcgatga agaccgacga cgtatttcat taggactgaa
     1321 acaatgtaag gcaaacccat gggaagcctt tgcatcggca cacacaaaat ccgataaagt
     1381 ctcgggtaaa atcaagtcaa ttacagattt tggtatcttt attggccttg atggtggtat
     1441 cgatggtttg gttcatttat cggatatcgc atggaacgaa agtggtgaag aagctgtgcg
     1501 tcgatataag aaaggcgaag aaattgaagc ggttgtattg gcggttgatc cagaacgcga
     1561 acgtatttcg ttggggatta agcagctcga aggtgatccg tatgccagtt atacaggaga
     1621 acacgctaag ggcagtattg taaccggtac gattttgtcg gttgatgcaa aaggcgcaac
     1681 aatacaattg gcggagggag tcgaaggtta tctgaaggcg agtgaactgg ggcgtgatcg
     1741 tgtagaagat gcacgcactg tgctaaaaga aggcgatgcg atagaggcta aatttacggg
     1801 cgtcgatcgt aaaacccgac agattacctt gtctattaaa gcaaaagatt ccgaagagga
     1861 acaggaagct atgcgtgagt taacgcgcca agagggtgca agtccaacgc tgggtgatct
     1921 gttcaaagag aaaatggaaa gcaaagataa aagggagtag acactaaaat gcgtctctta
     1981 agcataatca cattaacatt tgcggttttg cttggggtga gctttgcaat tctgaacgca
     2041 ggctttgtga cagtacatta ttatgttggc acaaaaagcc tgcctttgtc cttactcatt
     2101 ttaggtgcgt ggatattcgg tattatcgtt ggtttgctga tatccgcgtt taaaatcata
     2161 gggctacgaa tggaattgca gcgccagcat cgcgtgcctc gttagcgaat gtgggaattt
     2221 ttatttctat tgctgccttt ggcagcgctt tctggatggc ttattggtcg gcgtgcgagt
     2281 atgcgtcaag cgattcgcag actcgcaggc ttaaacaagc ttttagatca ttcttcaaat
     2341 aaaaaagcag tcgatgtttt agtgcagctc atggattcta atcaagaagc ggtagaaacg
     2401 cacttggcat tgggtggact atttaggcgt caaggagaag tcgacaaagc cattggtatt
     2461 cacgagagat tgatagcgaa accctcctta acagaagaac agcgtgcatt atcattatta
     2521 gaattaagtc gcgattatat gcgagctggg gtgttggatc gggcagaaag tgtattatta
     2581 gaactgattg agcttggata cgagctcgat gcgggatttc aaggtctttt agacgtttat
     2641 cagcagagta aagattggct aagagccatc caaacggcac aacagtggca aacaaagaaa
     2701 aatcgtaata tgagttatca tattgcgcat ttttattgtg agcttgcaga gcaacaatgg
     2761 ttaaaaggtg agcgtgttga tgcttatcag catttaaaag aagccctggt aacgaataaa
     2821 cgctgtgtac gagccagttt gttattaggg aatattgaag cacaagcggg acgctataga
     2881 gtggccatta gagcctataa gcaaatacag aagcaagatg aggccttttt atctgaaatt
     2941 gttttacctt tagcacgctg ttacgaaagc cttcataatg ccgagggtat gatcaaatat
     3001 tttgatgaaa acttacaaaa acatctgacg acttcagcgg ttttagcttt tgcagagtgg
     3061 ttgaacaaaa gtaagggtga gtctgaagcc atgcggtttg ttgtacaaca tttacgcaaa
     3121 aatccatcat tacggggtct tagccgattg gttcaattta gtcttgcgcg tgcggagggt
     3181 tctctgcagc aagatttgtt gttgtttcaa gaattaa
//
LOCUS       DAORVT010000131         3196 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_26225_length_3196_cov_2.899395, whole genome shotgun sequence.
ACCESSION   DAORVT010000131 DAORVT010000000
VERSION     DAORVT010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26225_length_3196_cov_2.899395"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..770
                     /gene="pyrH"
                     /locus_tag="PK389_03410"
     CDS             39..770
                     /gene="pyrH"
                     /locus_tag="PK389_03410"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HQY22786.1"
                     /translation="MGHSRMSQPIYKRILLKFSGEALLGSKPFGIDPAVLDRLAFEIG
                     ELVKRKVQVGLVIGGGNFFRGEALSAVGMGRITGDQMGMLATVMNALAMRDALERAGF
                     TTRIMSAIHMSGVVDYYDRRKAAHHLNKGRVVLFAAGSGNPLVTTDSAASLRAIEIDA
                     DILLKATNVDGIYTSDPRKDPHATRYSVLTYDEALKKELGVMDLTAFCQCRDHHMKIR
                     VFSLKKEGALLRIVDGLDEGTLVTD"
     gene            780..1337
                     /gene="frr"
                     /locus_tag="PK389_03415"
     CDS             780..1337
                     /gene="frr"
                     /locus_tag="PK389_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002536380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HQY22787.1"
                     /translation="MLKKIEQDTEIRMNKAVESLSHGLAKLRTDRAHPSILESVSVLY
                     YGNPTPLNQVASISIEDARTLSVTPFDKTAIQAIDKAIRTSDLGLNPTTAGTVIRVML
                     PPLTEERRRDLVKHVRSEAEEARVVVRNIRRDANAEIKQLLKVKKITEDDERRATDIT
                     QKLTDRFVAEIDKLVTFKETELMRV"
     gene            1337..2086
                     /gene="uppS"
                     /locus_tag="PK389_03420"
     CDS             1337..2086
                     /gene="uppS"
                     /locus_tag="PK389_03420"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HQY22788.1"
                     /translation="MKLPRHVAVIMDGNGRWAKKRLLPRVAGHQQGLESVRAIITTCV
                     KKEIACLTLFTFSSENWRRSENEVSFLFGIFLRSLRNETKSLHENNIRLKIVGDRSTF
                     SEELIAAIVTAEQTTEKNTGLQLNIAFNYGGRWDIVEASRALAKQVAAGTLLPEDISA
                     ENFGEHLAFGDCPEPDFFIRTSGEYRISNFLLWQLAYTELYFTDTYFPDFREAQFEDA
                     LIAYSKRQRRFGFSEEQPDPSLNFMSHKEYA"
     gene            2079..2909
                     /locus_tag="PK389_03425"
     CDS             2079..2909
                     /locus_tag="PK389_03425"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HQY22789.1"
                     /translation="MLKTRIITATILIPLVIFGTLFLPSLAFAVISGVVFGHGLWEWS
                     RLAGFQSVFSRIICLILMPLGVCLLLAILQWSSVQLQLGRWLFQEGVPLLILIFWLMA
                     GFSVAYYPRLEEFWKSKPMGVVAGCLVLVPSWFLLVALQHRNPWLVLYILSLIWVCDI
                     AAYFVGKRFGKHTLAPKLSPGKTWEGVLGAYAGGLLVVIVGYLYLDQDLSLFSWVMLG
                     LICITFSIVGDLFESLFKRLRNLKDSGTLLPGHGGVLDRIDSITATVPIFAIGFMFFS
                     "
     gene            complement(2979..>3196)
                     /locus_tag="PK389_03430"
     CDS             complement(2979..>3196)
                     /locus_tag="PK389_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22790.1"
                     /translation="KKELSPKKNLQEQLKAAQNGLRKTPVSSQPVKPQLAGWQVELQQ
                     KKAAAAAKVKETETPKPEDNTSKRPSK"
BASE COUNT          806 a          595 c          786 g         1009 t
ORIGIN      
        1 aattttgttg cagaggttat ggcgcaaatt cggtaattat ggggcattca cgcatgagcc
       61 aacccatcta caaacgcatt ttgttaaaat tcagcggcga ggcgctactc ggtagtaaac
      121 cttttggtat tgatcccgct gtcttagatc gcttagcgtt cgaaattggc gaacttgtta
      181 aacgtaaagt gcaagtggga ttggtaatcg gcggtggcaa ttttttccga ggtgaggcat
      241 tatccgcggt cggaatgggt cgaattacgg gcgatcaaat gggtatgctc gccacggtga
      301 tgaatgcact tgctatgcgt gatgccttag agcgcgcggg ttttacgaca cgcatcatgt
      361 ctgctattca tatgagtggc gttgttgatt attatgatcg acgtaaagca gcccatcatt
      421 taaataaagg gcgtgtggtt ttgtttgcag cgggttcggg taatcccttg gtgacaaccg
      481 attcggctgc aagcctaaga gccatcgaaa tagacgcgga tattttacta aaagccacca
      541 atgtcgatgg tatttatacg agtgatcccc gaaaagatcc tcatgccacg cgttatagcg
      601 ttttaacgta tgatgaagct ttaaaaaaag aattaggcgt gatggatttg acggcttttt
      661 gtcaatgtcg ggatcatcat atgaaaattc gggtttttag tttaaaaaaa gaaggggcgc
      721 ttttgcgtat tgtcgatggc ttagatgaag ggactttggt aactgattag ggtgaggcta
      781 tgctgaaaaa aattgagcaa gacacagaaa tacggatgaa taaagcagtc gaaagcctga
      841 gccatggttt ggcgaaatta agaacggatc gcgcacatcc cagtatatta gagtcggttt
      901 ctgttctcta ttatggtaac ccaacacctc taaaccaagt ggcgagcata tcgatagaag
      961 acgcgcgtac gctttcggtt accccctttg ataagaccgc tattcaggcc attgataaag
     1021 cgattcgcac atcggattta ggtttaaatc cgacaacagc agggacagtt attcgcgtta
     1081 tgctaccacc cttaacagaa gaacgtagac gcgatttggt taaacatgta agaagcgagg
     1141 cagaagaggc acgtgttgtg gtgcgtaata ttcgacgtga tgcgaatgcg gaaatcaagc
     1201 aacttttaaa ggtaaaaaaa attacggaag acgacgagcg tcgtgccacg gatattactc
     1261 aaaaattaac cgatcgattt gttgcagaaa ttgataagtt ggtcaccttc aaagaaacag
     1321 agctaatgcg cgtttaatga aacttccacg ccatgtcgca gttataatgg atggtaatgg
     1381 tcgctgggca aaaaagcggc tgttgccgcg cgttgctggg caccagcagg gtttagaatc
     1441 tgttcgcgcc attattacaa cctgtgttaa aaaagaaatt gcgtgtttaa cgttatttac
     1501 ctttagtagc gaaaactggc ggcgttctga gaatgaagtg agctttctat ttgggatttt
     1561 tttaagatct ttacgtaacg aaaccaaatc attgcatgaa aataacatac gattaaaaat
     1621 agttggtgat agaagtacgt tttcggaaga attaatagca gctatagtga cagctgagca
     1681 aacaaccgaa aaaaatacag ggttacaact gaatattgct tttaattacg gggggcgatg
     1741 ggatattgta gaggcatcgc gtgcattagc taaacaagtc gctgcaggga cacttttgcc
     1801 cgaagatatt tctgctgaaa attttggcga acatttagcg tttggcgatt gtcctgaacc
     1861 tgattttttt atccgcacca gtggtgagta ccggattagt aattttttgc tttggcaatt
     1921 agcttatacc gaattatatt ttacagacac ctatttccct gattttagag aagcgcaatt
     1981 tgaggatgct ttaatagcat actccaaacg tcaacgccgt tttggcttta gcgaagagca
     2041 acccgatccg tcgcttaatt ttatgtcaca caaagaatat gcttaaaaca cgcattataa
     2101 cagccactat tttaattccc ttagtaattt ttggaacctt atttttacct tccctggctt
     2161 ttgctgtgat cagcggtgtt gtttttgggc atgggttgtg ggaatggtca cgattagccg
     2221 ggtttcaatc ggtgtttagt cgtataattt gccttatttt aatgccactc ggtgtttgct
     2281 tgttattagc cattttgcag tggtcttcgg tccagttaca attgggcaga tggctgtttc
     2341 aggaaggcgt tcctttgcta attctgatct tttggttgat ggctggtttt tcggttgcgt
     2401 attaccctag attagaggaa ttttggaagt cgaaaccgat gggcgttgtt gcgggttgtc
     2461 ttgttttggt gccctcatgg tttttattgg tggctttgca gcatagaaat ccttggcttg
     2521 tgctgtatat tttgagtttg atttgggtct gcgatattgc cgcttatttt gtcgggaagc
     2581 gttttggaaa gcatacactg gcgccaaagc ttagtccagg caaaacctgg gaaggggtct
     2641 tgggagcgta tgctgggggc ttgttggttg ttattgtagg ctacctttat ttagatcaag
     2701 atctctcgct gttttcttgg gttatgttag ggttgatctg cattacgttt tctattgttg
     2761 gcgatttatt tgaaagttta ttcaaacgcc ttcgcaatct aaaagacagt ggcacgctct
     2821 tgccgggcca tggtggtgta ctcgatcgca tcgatagcat tacagccact gtgccaattt
     2881 ttgccattgg ttttatgttt tttagttaaa agcgatggag ttagagcctg cttctttttt
     2941 gaagattagc aggctacatt ttgactcctg cggatacttt atttactagg tctcttcgat
     3001 gtattatcct ctggtttagg cgtttcagtc tcttttacct tggctgccgc tgccgctttt
     3061 ttctgttgaa gttcgacctg ccatccagcc agctgcggct tcactggttg actgcttact
     3121 ggagtcttac gcaaaccgtt ctgtgctgcc ttaagttgtt cttggagatt tttcttcgga
     3181 cttagttctt tcttaa
//
LOCUS       DAORVT010000132         3193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_26266_length_3193_cov_3.398980, whole genome shotgun sequence.
ACCESSION   DAORVT010000132 DAORVT010000000
VERSION     DAORVT010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26266_length_3193_cov_3.398980"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(372..2399)
                     /locus_tag="PK389_03435"
     CDS             complement(372..2399)
                     /locus_tag="PK389_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HQY22791.1"
                     /translation="MDKVIYSHWHIERDEDNIAWLTFDKQDSKTNTIDLVVLEELLSV
                     IQSLETGTKPLGLVIDSSKKNGFCAGADIQQFKNTESLETIVNLIQQGQTVFNHLANV
                     SFPTVALIEGYCLGGGLELALACRYRIAIEDPKTKLGLPEVRLGIQPGWGGSVRLPRL
                     IGGLKAMDLILSGRTIEAKAARKMGVVDVSVPKRQGLVAARYFVKTQPKVRAANLIED
                     FTNRHFVRPLLGKYLLKAISAKVNRAHYPAPYAVLQHWIDQGVFHNAAFIAEANSVCQ
                     LAQSETARNLIRVFELQEKLKRLGSESSFRPEHVHVVGAGVMGGDIAAWCALKGIRVT
                     LQDQNTLAVAAALGRAKALFKKQLKEPHLVQAAMDRLMPDIKGCGIKTADLIIEAIIE
                     KAEAKKALFMTLASEAKKEAIFATNTSTIPLKALSEDPAIQQRIIGLHFFNPVARMPL
                     VEVIDTASTDPHLIQYGMSFVRAIEKIPLQVKSSPGFLVNRILMPYLLESVRLLELGI
                     PAVAIDKAVVEFGMPMGPIELADTVGLDVCLYGAESLAHYLKVSIPTELRKRVEAGNL
                     GKKTGQGFYQFKNGKIIRPKLPEHYRLPEDALDRLILSMMNEAVACLREGIVADADAL
                     DAGMILGTGFAPFRGGLMHYIAEQGESLLFQRLNLLAQRYGDRFLPDAGWR"
     gene            complement(2401..>3193)
                     /locus_tag="PK389_03440"
     CDS             complement(2401..>3193)
                     /locus_tag="PK389_03440"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HQY22792.1"
                     /translation="KSLLQFRPYFLKPILSLLKGLSDPIVNLSMGQTAENLVYEFGIT
                     REAMDAFSVQSHNRASAAQKEGLFEEITPLFDWQGGMIESDNGVREGSTVEKLATLKP
                     VFDKPFGSVTAGNSSQVTDGAAFLILASESSVKKYDLPVLGKIIDTAWAALDPAIMGL
                     GPVYAIQKLLQEQDLSLSDIDYWEMNEAFAAQMLACLKSLGSIPPDKLNVDGGAIAIG
                     HPVGATGARLVLHLLHVLKRNGAKRGIASLCIGGGQGGAMLVEAF"
BASE COUNT          941 a          793 c          564 g          895 t
ORIGIN      
        1 cttccgttgc ttgcaacatc cttacactcc ttattttctc cttaaagcct ctgtcatttt
       61 tcgaaactca ggggagtgta acatacagcc gatttttaag acaaaagcca tgcatgcctc
      121 atgcattgta aacaaaaaga aatcggaata gatatccgct aaataacgga taaagcacca
      181 atgggattaa aggaacccga taagtcgatt tgctgaattt aaatatatta agtcagtgcg
      241 taccagaacg tgcgcgaaat aacttgctct tttaagcgag ccaatgtctg agcgcagcga
      301 gttgggcgag tgaaaaaagg gcaagttatt tcatgcacgt tccttatata gctatataat
      361 tatcaatata attaccgcca tcccgcatcc ggtaaaaacc gatcgccata ccgttgcgcc
      421 aataaattca atcgttgaaa cagcaacgat tcgccttgct ctgctatata atgcatcagt
      481 cccccgcgaa acggcgcaaa tcctgtgccc aatatcatgc ccgcatctaa agcatccgcg
      541 tctgcaacaa tcccttctct taagcaagca accgcttcat tcatcatcga taaaatcaag
      601 cgatcaagcg catcttcggg taatcgataa tgttccggca attttggacg aataattttt
      661 ccatttttga attgataaaa cccttgaccg gttttcttgc ctaaatttcc agcttctaca
      721 cgttttcgta attcggtggg aatagatact ttcaaataat gcgctaaact ttctgcacca
      781 tacagacaaa catctaagcc tactgtatcc gcaagttcaa taggccccat cggcatccca
      841 aattccacga ctgctttatc aatagcaacc gcagggatac ccaattctaa taatcgaacg
      901 ctttccaata aatagggcat taaaatacga ttgaccaaaa acccaggtga acttttaacc
      961 tgtaatggga ttttttcaat cgcacgcaca aaactcatcc cgtattgaat taaatgagga
     1021 tccgtcgatg ccgtatcaat cacctctact aaaggcattc tggcaaccgg gttaaagaaa
     1081 tgcaatccga taatcctctg ttgtatcgca ggatcttcac ttaacgcttt tagagggatt
     1141 gttgaagtgt ttgttgcaaa tatcgcctct tttttcgctt cactcgctaa tgtcataaac
     1201 aaagcttttt tggcttccgc tttttcaata atagcttcaa taattaaatc tgcggtttta
     1261 atgccacaac ctttgatgtc gggcattaat ctatccatcg cagcttgtac caaatgcggc
     1321 tcttttaatt gttttttaaa caaggctttt gcacgcccta gcgctgccgc aaccgctaat
     1381 gtattttgat cttgcaaggt aacgcgtatg ccttttaatg cgcaccaagc ggcaatatct
     1441 ccacccataa cgcccgcacc cacgacatgg acgtgttcag gtctaaagct agactctgac
     1501 cctaaacgct ttaatttttc ttgcagttca aaaacccgaa ttaaatttcg agccgtttca
     1561 ctctgagcca attggcaaac agaatttgcc tccgctataa atgccgcgtt atgaaacaca
     1621 ccctgatcga tccagtgttg taaaacagca tagggtgcgg gataatgtgc acggtttact
     1681 ttagcagaaa tagcttttaa taaatatttc cctaaaagag gtcgaacaaa gtgccgattg
     1741 gtaaaatcct cgataagatt tgcggccctg actttcggtt gggtttttac aaaataacgg
     1801 gcagcaacca aaccctgacg tttcggcacc gatacatcca caacacccat tttacgcgcg
     1861 gcttttgctt ctattgtacg cccagacaaa attaaatcca ttgctttaag tccaccgatt
     1921 agtctcggaa gacgaacgct gccaccccat ccgggctgaa tgcctaatcg cacttcgggt
     1981 aaacccaact tagttttagg atcttcgatt gcgatacggt aacgacaagc caaggcgagt
     2041 tctaacccgc cccccaaaca ataaccctca atcaacgcca ccgttgggaa agacacattg
     2101 gcaaggtgat taaaaaccgt ttgaccttgc tgtatcaaat taacaatggt ttctaaagat
     2161 tccgtgtttt taaattgttg aatatccgca cctgcacaaa atccattctt tttggacgaa
     2221 tcgataacaa gccccaatgg ctttgtacct gtctctaaag attgaataac agataataat
     2281 tcttctaaca ccactaagtc tatggtattc gtttttgaat cttgcttatc aaaggttagc
     2341 cacgcaatat tatcttcatc acgctcgata tgccaatgac tataaatcac tttatccatc
     2401 ttaaaacgcc tccactaaca tcgcgccccc ttggccacca ccgatacaca agctcgcaat
     2461 cccacgcttc gcaccattcc tttttaacac atgcaataaa tgcaaaacca atcgagcgcc
     2521 tgttgcgcca acaggatgcc caatggcaat cgctccacca tcgacattca atttgtcggg
     2581 tggaattgat cctaaagatt ttaaacacgc caacatttga gctgcaaaag cttcattcat
     2641 ttcccagtag tcaatatccg acagggacaa gtcttgttct tgaagcaatt tttgaatggc
     2701 atagacggga cctaaaccca taatagccgg atcaagcgcg gcccaagcgg tatctataat
     2761 ttttcccaaa acaggtaaat catatttttt tacgctcgat tcgctcgcca aaatcaaaaa
     2821 agctgcgcca tcggtcactt gcgaactgtt ccccgcggtt acactaccaa agggtttgtc
     2881 aaaaacaggt tttaaggttg ctaatttttc tacagtagac ccctcgcgta ccccgttatc
     2941 cgattctatc atgccacctt gccaatcaaa taagggcgtt atttcctcaa atagaccctc
     3001 tttttgggca gcgctggctc tgttatggct ttgaacagaa aaagcatcca tcgcttcgcg
     3061 cgtaatccca aattcgtaaa ctaaattttc ggcggtttgt cccatcgaca aattaacaat
     3121 cggatcactt aagcctttca acaatgaaag aatgggtttt aaaaaataag gtctaaactg
     3181 tagtaatgat ttt
//
LOCUS       DAORVT010000133         3187 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_26363_length_3187_cov_2.960089, whole genome shotgun sequence.
ACCESSION   DAORVT010000133 DAORVT010000000
VERSION     DAORVT010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26363_length_3187_cov_2.960089"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PK389_03445"
     CDS             <1..184
                     /locus_tag="PK389_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22793.1"
                     /translation="EFGMTPQVDPGKIIYLIQKMPTVYSLEGPQKFRFKLELSNVKTR
                     VEEVGKLMNALGTEQK"
     gene            complement(282..1175)
                     /locus_tag="PK389_03450"
     CDS             complement(282..1175)
                     /locus_tag="PK389_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22794.1"
                     /translation="MFSKTYNCIFNKFVQWLTTDRPYEGIPLCDFERIRYELRPCDVL
                     LIEGRSRVSDIIRAITQSSWSHSVLYIGRLHDIEDPKIRNLLSVHFKGSADAQLIIEG
                     IMGQGIIVSNLSSYANDHIRLCRPKGLSRRDAQNVIAFSIQRLGSAYDVRQILDLARF
                     LLPWTIMPRRLRSKLFELNAGESTKTVCSSMLAEAFLSVEFPILPLVKQHAEQGIELI
                     ARNPKLYTPRDFDYSPYFDIIKYPFVEFADYAMYRKLPWNREGLMSHDGIGVRSTGPT
                     LFEEKPVLKKKKKKDDPIESD"
     gene            complement(1232..2320)
                     /locus_tag="PK389_03455"
     CDS             complement(1232..2320)
                     /locus_tag="PK389_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010958086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HQY22795.1"
                     /translation="MAITLETLVLIIAAVIFVFMCLQCGFLIWYSQLNTRQQHDLKEN
                     MIDRLAHLTQTLEQRLNENLLRQSKESGEIQKMVQEQLKEISYHVEKRLSEGFEKTTA
                     TFTDIVKRLALIDDAQKKLSELSSNVVSLQEILVDKRSRGAFGEMQLKNLVENMLPKD
                     RCAFQHTLSNGMRADCILFLPEPTGHIVIDSKFPLENFQLMTDFDSTTPERKKAQQDF
                     KVDIKKHINDIASKYIIAGETTNGAVLFIPAEAVFAEIHAHFPDLVEFAHRLNVWLAS
                     PTTLMAILTTARAVLKDFATKEHIHVIQEHLRHLSEDFNRFEMRMDNLVKHIDQAQKD
                     AQQVHTSAKKISSRFIQIEKADLPLTIE"
     gene            complement(2372..>3187)
                     /gene="rpoS"
                     /locus_tag="PK389_03460"
     CDS             complement(2372..>3187)
                     /gene="rpoS"
                     /locus_tag="PK389_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HQY22796.1"
                     /translation="TDIYLKEIGITPLLSKEEEVFYGRLVQQGDPIARKRMIESNLRL
                     VVRIAKRYLKSGMAILDLIEEGNIGLMRAVEKFDPERGFRFSTYSAWWIQQTIERAVM
                     SQTRTIRLPVHMVKRLNKCLKTSHTLSKNLDHDPTVEEIAVAWGKTIEEVEKMFHLNE
                     RVISIDTPISDEISKPLLDTMSNEQNDPLDNLNRHKLQQNLDKWLSRLNMKQRAVVER
                     RYGLNGFDQMTLDKTGTEIGLTRERVRQLQSEALKLLRFQIESQGNDLESLLI"
BASE COUNT          872 a          608 c          682 g         1025 t
ORIGIN      
        1 tgaatttgga atgacaccac aggttgatcc ggggaaaatt atttatttaa ttcaaaaaat
       61 gccgacggtt tatagcctag agggacctca aaaatttcgt tttaaattag agttaagtaa
      121 tgttaagact cgggtggaag aagtcggaaa gttgatgaat gctttgggca ctgagcaaaa
      181 atagatttta aataattatt ttgattcgct actgaactcg cttcgctcaa acatgcatcc
      241 gctcaccaaa ttaattattt aaactctatt tttgcagtgc cctaatctga ttcaatcgga
      301 tcatcttttt tctttttttt cttaagcacg ggtttttctt caaaaagtgt gggtccagtc
      361 gatcgaacgc cgatgccatc gtgactcatt aatccttctc gattccaagg caattttcga
      421 tacatcgcat aatctgcaaa ttctacaaaa gggtacttaa taatatcgaa ataaggcgaa
      481 tagtcgaaat cccttggggt atagagcttt gggttacggg cgataagttc gatgccttgt
      541 tctgcatgtt gtttaaccaa tggtaaaatc ggaaactcca cactcaaaaa tgcttcggcc
      601 aacatggaag aacaaacggt tttggtactc tcacccgcat tgagctcaaa taattttgag
      661 cgcaatcgac gaggcataat cgtccacggc agcaagaagc gtgccaaatc cagtatttgg
      721 cgcacatcgt atgctgagcc taatcgttga atagaaaaag caatcacatt ttgtgcatcg
      781 cgcctggaaa gtcctttggg tcgacagagg cgaatatgat cgtttgcata gcttgaaaga
      841 ttgctgacaa tgatgccttg tcccatgata ccttcgatta ttagctgggc atcagcagag
      901 cccttaaaat ggacagacaa tagattgcga attttaggat cttcaatatc gtgcaatcga
      961 cctatatata acaccgaatg agaccaagag ctttgagtga tagcacgaat gatatcgctg
     1021 accctgctcc gaccttcaat taacaaaaca tcacaaggtc gtagttcata gcgaatacgc
     1081 tcaaaatcgc ataaaggaat gccttcatag ggacgatcag tggtcagcca ctgtacaaat
     1141 ttgttaaaaa tacaattata agttttagaa aacatcggtt ttttccttag gcgttcttta
     1201 aatttagtgc atctaaggtt aaatagccaa cttattcaat agtgagtggc aaatcggctt
     1261 tttcgatttg gataaaccga gaggatattt ttttggcgga agtatggact tgttgtgcgt
     1321 ctttttgggc ttgatcaatg tgttttacga gattatccat tcgcatttca aaacgattaa
     1381 agtcttcgct gagatgacgt aaatgttctt gaatgacgtg aatatgttcc ttcgttgcaa
     1441 aatcttttag aacagctctc gctgtcgtta aaatggccat tagcgtagtg ggggaggcaa
     1501 gccagacatt taatcgatgt gcaaattcca ctaaatctgg aaagtgcgca tggatttctg
     1561 caaatacggc ttcagccggg ataaaaagta cagcgccatt agtggtctcg ccggcaataa
     1621 tatatttgct ggcgatatcg ttaatatgtt ttttaatatc gactttaaaa tcttgttgcg
     1681 cttttttgcg ctcgggcgtt gtagaatcga aatccgtcat taattggaaa ttttctaagg
     1741 gaaatttaga atcgatgaca atatgccctg tgggttcggg taaaaataaa atacaatcgg
     1801 cacgcatgcc attgctgagg gtgtgctgaa acgcgcatct gtctttgggt aacatgtttt
     1861 cgacaaggtt tttcagttgc atttcgccaa aagcaccacg tgatcgttta tccactaaaa
     1921 tttcttgtag gcttacgaca tttgaggaaa gctctgagag ttttttttgt gcatcgtcaa
     1981 tcaatgcaag tcttttaacg atatctgtaa aagttgcggt tgttttttca aaaccctcac
     2041 tgagccgttt ttcaacatgg tagctaattt ctttaagctg ttcttgaacc attttttgaa
     2101 tttcgccgga ttctttgctc tgtcttaaga gattttcgtt gaggcgttgt tctagggttt
     2161 gcgtgaggtg agccaggcga tcgatcatat tttctttcag atcgtgttgt tgtcgagtat
     2221 tcagttgact ataccaaatt aaaaagccgc attgtagaca cataaacacg aaaatgacag
     2281 ccgcgataat aagaactaag gtttcaagtg tgatggccat gttgggatcc ctgatttttt
     2341 ttcagtatac ccgatttcgg ttggatttga tttatattaa gagagattct aaatcattcc
     2401 cttggctttc gatttggaag cgcaacaatt taagcgcctc gctttgtaat tgtctaacgc
     2461 gttcgcgtgt aaggcctatt tcagtacctg ttttatcaag ggtcatttga tcgaatccat
     2521 ttaagccata acggcgttca acaactgctc tttgtttcat gttaaggcga gacagccatt
     2581 tgtctaaatt ttgttgtaat ttgtgacgat tcagattatc caagggatca ttttgttcgt
     2641 tactcattgt gtctagcagc ggtttgctga tttcatctga aatgggtgta tcaatggaga
     2701 taacgcgttc gtttaaatgg aacatttttt cgacttcttc tatcgtttta ccccaggcta
     2761 ctgcgatttc ttcaacggtt ggatcgtgat ctaaattttt agataaggta tggcttgttt
     2821 ttagacattt attcagccgt tttaccatat gcacgggtag acggatggtg cgtgtttgac
     2881 tcatcacagc gcgttcgatg gtttgctgga tccaccatgc actgtaggtg gaaaagcgaa
     2941 atccgcgttc gggatcgaat ttttccactg cccgcatcaa cccaatatta ccttcttcaa
     3001 ttaaatctag gatggccatg ccactcttaa gataacgttt ggcaattcta acgacgaggc
     3061 ggaggttgct ttcgatcatc cgttttcggg cgatgggatc tccttgttga accaatcgac
     3121 catagaatac ttcttcttct ttcgagagta ggggagtgat gccaatctct tttaaataga
     3181 tatcggt
//
LOCUS       DAORVT010000134         3162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_26724_length_3162_cov_4.017058, whole genome shotgun sequence.
ACCESSION   DAORVT010000134 DAORVT010000000
VERSION     DAORVT010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26724_length_3162_cov_4.017058"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /locus_tag="PK389_03465"
     CDS             <1..1168
                     /locus_tag="PK389_03465"
                     /inference="COORDINATES: protein motif:HMM:NF015197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HQY22797.1"
                     /translation="IGAGMLALPLSTANSGFIPSGFAFLVCWFFMTVAALLLLEVNLR
                     FSGDKDLISMTHAIFGRFGKMIAWIVYLLFLYALIVAYLTGSSAWVLNALEKFHLNIS
                     SNIVVMSLIFVFGLIISYGTVVVENINRYLMSGLVLTYILLIVSASPSVETAKLGVMD
                     FTDVQLTLPLIITAFGFAPIIPSLTHYLNRDVLALRYVVMLGSFIPLVIYLLWEWVAL
                     GIIPLTGVNSFETLIQHHDDGTGVAVALEHITGNAWITQSSRWFTLCAIFTSLLGVSL
                     ALLHFLADGLGLQKKTRLHRFFLLGCVYLPPLLIVLFYPSGFSHVLSLAGILAALLFG
                     IFPACMVWCTRYSRNKKFERGYQVFGGKLMLVTVIIFFCYIVYVEITNCIICRS"
     gene            1156..1950
                     /locus_tag="PK389_03470"
     CDS             1156..1950
                     /locus_tag="PK389_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005357054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY22798.1"
                     /translation="MSLVVTWQTPACQLKAEQLAQTLNLSLAKSAGYNFLENTSTLLH
                     VTEDRIELVSKHDKTPHVIFAEFLTGPFGYRWLKGGGRNQAIARAVGLKAGAKQITVL
                     DATAGLGRDAFILASLGCRVQLVERSPIIATLLIDGLLRASQDAKGAAVTQFMTVVSD
                     DAKKILEAITPENSPDVVYLDPMFPHRDKSALTKIEMRLIRNIVGDDKDADMLLPLAL
                     KKARKRVVVKRQRHAPVLQGSAPSFIVKGKSNRYDIYLTGGHSNLQ"
     gene            1947..3062
                     /locus_tag="PK389_03475"
     CDS             1947..3062
                     /locus_tag="PK389_03475"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HQY22799.1"
                     /translation="MMNFRGQLTRYSEGSVQELWAISYPLILSILSVNMMLFFDRLIL
                     ARYNTGAMNAAVLAWLIFSIFQLGTIGIASIAEVFVGQYNGAKKLNKMGEPVWQMIWF
                     SLMTGFIFIPLGLFAGPLFISNPDYVAEGIPFFKILMLFGPTFPITAAVSSFFIGRGR
                     VKLVMFTTVLSNVLNIALDFLLIFGVDGVVSALGAKGAAIATGVAQTVQVVVLLAVFL
                     RGRHRETHGTHRWKFKPKLFSSILRIGIPNALANVMDAIAWSVLIQILAFVSVNHVTA
                     FTIGDSFSVLFVVGFLGLQKGITTVAANYIGANREEVLSRTLRSSIKIILIIMLFLII
                     PFFFFSEKLVDLFFNVNSPLTLNETLKNQVAIALRWL"
BASE COUNT          761 a          572 c          756 g         1073 t
ORIGIN      
        1 tataggtgct gggatgcttg cattaccctt aagtactgcc aatagtggat ttatcccaag
       61 tggttttgct tttttggtgt gttggttttt tatgacggtt gccgctttat tgctgctcga
      121 agtgaacttg cgattttcgg gtgacaaaga tttaatttcg atgacacatg caatatttgg
      181 tcggtttgga aaaatgatcg cctggattgt ctatcttttg tttttatatg ctttgattgt
      241 tgcttatctg acgggtagta gcgcttgggt tttaaatgcg cttgaaaaat ttcatctcaa
      301 tatatcctca aacattgtgg tgatgagttt aatttttgtg ttcggattaa ttatttctta
      361 tggcactgta gttgtcgaaa atattaaccg gtatttgatg tctggtttag ttttaaccta
      421 tatcctgctt attgtttcag catcgcccag tgttgaaacg gctaagcttg gcgtgatgga
      481 tttcacagat gttcagctaa cgttgccttt gattattacc gcttttggat ttgcgcctat
      541 tatccccagt ctaacgcatt atttaaatcg tgatgtattg gccttgcggt atgtggttat
      601 gctgggcagt tttattccat tggttattta tctgttgtgg gaatgggttg cgctgggcat
      661 aatcccgctg acgggggtta atagttttga aacattgata caacatcacg atgatgggac
      721 aggtgttgct gttgccttag aacacattac ggggaatgcc tggattacgc aaagtagccg
      781 atggttcacg ctatgtgcta tttttacctc tttactgggc gtatccttag ccttgcttca
      841 ttttctagcc gacggattag ggttacaaaa aaaaacaaga ttacaccgct tttttttact
      901 aggctgcgtt tatctgcctc ctttgttaat tgttttattt tatccctcgg gttttagtca
      961 tgttttaagt ttagcgggta tacttgcagc gctattgttt gggatttttc ctgcctgcat
     1021 ggtgtggtgt acgcgatatt cacggaataa aaaatttgag cgcggttatc aagtttttgg
     1081 tggaaagctc atgttggtga cagttatcat tttcttttgt tatatcgttt atgtggagat
     1141 tacaaactgt atcatttgtc gctcgtagtt acttggcaaa cgcctgcctg ccagctgaaa
     1201 gcagaacagc ttgctcaaac acttaatttg tcactggcta aatcagcagg atataatttt
     1261 ttagagaata catccaccct gctacatgta acagaagatc gcatcgaatt ggtttctaaa
     1321 cacgataaaa caccgcacgt tatctttgca gaatttttaa cgggcccgtt cggataccgc
     1381 tggctaaaag gcggtggtag aaaccaagcg atcgcgagag cggttgggtt gaaggcgggt
     1441 gccaaacaaa tcacagtctt agatgcaaca gcagggttag ggcgggatgc ttttatcttg
     1501 gcgtcgcttg gatgcagggt acagctggtc gagcgttccc ctatcatcgc aacattatta
     1561 atagatggtc tgttgcgtgc gagccaagac gcaaaaggtg cagcggtgac gcaatttatg
     1621 acggttgtta gcgatgacgc aaaaaaaatt ttagaagcca taactccaga aaacagccca
     1681 gatgtggtgt atttagatcc catgtttccg catcgcgaca aaagcgcctt gaccaaaatc
     1741 gaaatgcggc tcatacgtaa tatagtggga gacgataagg atgcggatat gctgttgcct
     1801 ttagcgctta agaaggcgcg aaagcgggtt gtggtgaagc ggcaacggca tgcgcccgtc
     1861 ctgcagggaa gtgctcccag ctttattgtc aaaggcaaaa gtaatcgata tgatatctat
     1921 ctgacaggag ggcactctaa tttacaatga tgaactttcg cggccagctc acccgctatt
     1981 ctgaaggcag tgttcaagag ctctgggcta tttcgtatcc tctgatctta tcgatactgt
     2041 ctgtcaatat gatgcttttt ttcgaccgtc ttatcttggc ccgatacaac accggggcga
     2101 tgaatgcagc ggtgcttgct tggttaatat tcagcatttt tcagttgggt actattggta
     2161 ttgcttctat tgcagaggtt ttcgttggtc agtataacgg tgcaaagaaa cttaacaaga
     2221 tgggagaacc tgtctggcag atgatctggt tttctctgat gaccggattt atttttattc
     2281 cgctgggtct ttttgctggc cccctcttta tttcgaatcc agattatgtt gcagaaggca
     2341 ttccattttt taaaatctta atgttattcg ggcctacttt tccaatcact gccgccgtat
     2401 cttctttttt tataggacgt gggcgtgtaa agcttgttat gttcacgacg gtgctcagta
     2461 atgttttaaa tattgcattg gattttttat tgatttttgg ggtggacggt gttgtgtcgg
     2521 cattaggtgc caagggtgct gctattgcaa cgggtgtggc acaaacggtc caggtggtcg
     2581 tgttgttagc cgtttttttg cggggtcgac atcgagaaac acatggcaca catcggtgga
     2641 aatttaagcc aaaactattt tcaagtatct tgcgcattgg tattcctaat gcattagcaa
     2701 acgttatgga tgcaatagcc tggagtgttt taattcaaat tttagctttt gtgagtgtaa
     2761 accatgtgac ggcatttacg attggcgata gcttttctgt attgtttgtt gtggggtttt
     2821 tgggcttgca aaaaggtatc acaacagttg ctgccaatta tattggcgca aatcgcgaag
     2881 aagttttgtc tagaactttg cgctccagta ttaaaataat tttgattatt atgttgtttc
     2941 tgattattcc ctttttcttt ttttctgaga aactagtcga tctttttttt aatgtcaatt
     3001 cacccttaac attaaacgag acgttaaaaa atcaagtggc gatagcgttg agatggctat
     3061 aagtatattt tatgttcgat gcggtttcat ggatgattag tggaatatta atcgcagcgg
     3121 gtgatacaaa atttgtgatg tggataaaca gcgtgaacgc tt
//
LOCUS       DAORVT010000135         3152 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_26870_length_3152_cov_3.626736, whole genome shotgun sequence.
ACCESSION   DAORVT010000135 DAORVT010000000
VERSION     DAORVT010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26870_length_3152_cov_3.626736"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="PK389_03480"
     CDS             complement(<1..1022)
                     /locus_tag="PK389_03480"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HQY22800.1"
                     /translation="MLPTIEIIPSYHKRFLSGYPWLYANEIKQSQSTKILEPGELVAV
                     AHQGKRVATGYFNRHSLISFRALSSSDTNIDVTFFKQRLQQSLALRERFYNMPYYRLV
                     HAEADGLPGLIIDRFDSVFVIQMNTQGMEKLKNLLVEALQALFSPTTLYFRNDSAIRT
                     LEGLAITEPKVIGKPLSTLTVIENNMTFEIDMTSSQKTGWFFDHRENRALIAKLAPGK
                     TVIDYFCYSGGFGLQAAKQQAKRVIGIDRSEGALKNAERSANLNHLSELCEFHCRDTF
                     NDMDDRLQKGEIFDIVVLDPPAFVKNKKDLIPGLKGYEKLLTKGLPLISPSGFLLIAS
                     CSYHVK"
     gene            1150..2712
                     /gene="murJ"
                     /locus_tag="PK389_03485"
     CDS             1150..2712
                     /gene="murJ"
                     /locus_tag="PK389_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008291116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HQY22801.1"
                     /translation="MSKELLKSTSVVGGMTLISRVLGFVRDMVAAHFFGAGFGYDAFI
                     VASKIPNFMRRLFAEGAFSQAFVPILSEYRTQNTQAEVEQFVSRVFGCLSTILAGVTI
                     LGILAAPLLVMLFAPGFATSGSGVRLALASDMLRITFPYLLFISLTAFAGGILNCYGR
                     FAVPAFTPVFLNIMMILATVVLAPHLSEPVMALAWGMFAGGVVQLLFQVPFLRRLKLL
                     SRPEFYWQDPGVRRILKLMLPALLGVSVNQLNLMLSSVFASFLPVGSVSWLYYAERLM
                     EFPLGGFGVALATVVLPRLSRHHTKAEDEHFSATLDWGVRWVMLIGLPAALGLALLAG
                     PLLTTLFQSGQFTDQDVISSQMCLVAYSLGIMGFMLVKIFASAYYAKQDVKTPVRMAV
                     VTVTANLLFSLIFMKFLAHTGLALATSMAALLNATLLGYGLFKRHIYTLQPGWTFLAL
                     QLLLALSAMGIVIRMFSPDLVAWFAMDVTERVVTLFGLIITSGLAYIATLFLSGLRTN
                     HILGRAISEGDS"
     gene            2709..>3152
                     /locus_tag="PK389_03490"
     CDS             2709..>3152
                     /locus_tag="PK389_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HQY22802.1"
                     /translation="MSDYKETLNLPNTAFPMKANLAEREPAVLKRWNEIKLYQQLAEA
                     GKNKINKDKTKLFILPDGPPFANGHIHIGHAVNKILKDFILKSKTLNGYYAPFVPGWD
                     CHGLPIELNVEKEVGKPGVKLDAKAFREKCRLYAMKQINLQRDAFI"
BASE COUNT          840 a          578 c          722 g         1012 t
ORIGIN      
        1 tctttcacat gataagaaca agaagcgatt aataaaaacc cactcggtga gattaatggc
       61 agacccttgg ttagcaattt ttcgtaaccc ttaaggccag ggatgagatc ttttttattt
      121 ttcacaaaag cagggggatc taaaacaaca atatcaaaaa tctctccctt ttgcagacga
      181 tcgtccatgt cgttaaacgt atctcgacag tggaattcac aaagttcaga aagatgattc
      241 aaattggcag agcgttctgc gtttttgagt gcaccttcac tgcgatcgat gccaatcacg
      301 cgcttggcct gttgtttggc ggcctgtaag ccaaacccac cgctataaca aaagtagtca
      361 atcaccgttt tgccaggtgc cagtttggca attaatgcgc gattttcgcg atgatcaaaa
      421 aaccagcctg ttttttggct ggatgtcata tcgatctcga aggtcatatt attttcaata
      481 acggttaagg tagaaagggg tttgccgatc acctttggct cggtgattgc taagccctca
      541 agtgtgcgaa tagcagaatc gtttctaaag tagagtgtcg ttggcgaaaa taaagcttgg
      601 agcgcttcta ctaatagatt tttaagtttt tccatacctt gtgtattcat ttggatcaca
      661 aatacgctat caaaacgatc aatgattaag cctggcaaac catcggcttc tgcatgaacg
      721 agtctatagt aaggcatgtt ataaaaccgt tctcgcagcg ctaggctctg ttgcagacgt
      781 tgtttaaaaa aagtgacatc aatattcgta tccgagcttg aaagcgctct gaaagaaatc
      841 aaggagtgtc gattaaagta gcccgttgcg acacgcttac cttggtgtgc gactgcaacc
      901 aattcgccag gctctaaaat tttagttgat tgactttgtt tgatttcatt ggcatataac
      961 cagggataac ctgataaaaa ccgcttatgg taactgggaa tgatttcgat ggttggtaac
     1021 atgtgactcg tatcctaata atctgtaaga ctttgtctag cattgtgatg ttggccaggt
     1081 tactattaac agtcttcaaa ttcaacgatt aaatagggta atagcaacca gcatgcgttt
     1141 tttaaacaga tgagcaaaga actcttaaaa tcgacatcag tagtcggagg aatgacttta
     1201 atttctcgcg tcttagggtt tgttagagat atggttgcgg cgcacttttt tggtgcgggt
     1261 tttgggtatg atgcttttat tgtggcctca aaaatcccca attttatgcg ccgattattt
     1321 gcggaaggag cattttcgca agcatttgtg cccattttat cggaatatcg cactcaaaac
     1381 acacaagcag aagtcgagca atttgtgagt cgtgtcttcg gctgcttaag tacgatttta
     1441 gcgggtgtta ctattttggg catattggca gcgccactat tggtgatgct atttgcgccg
     1501 ggttttgcga caagcgggtc aggtgtacgt ttagcgctcg cctcagacat gttacgcatt
     1561 acttttcctt atcttttgtt tatttcttta accgcttttg caggtggcat attaaattgt
     1621 tacgggcgat ttgctgttcc tgcatttaca cccgtttttt taaatataat gatgatacta
     1681 gcaacggttg tattagcacc gcatttatcc gaaccggtga tggcattggc atggggcatg
     1741 tttgcaggtg gggttgtaca attgttgttt caggtgcctt ttttaagacg gttgaaattg
     1801 ctctcgcgcc cagaatttta ttggcaagat cccggtgttc gacgaattct aaagttaatg
     1861 ttgccagcgc ttttaggcgt ttctgtgaat caattaaacc tgatgcttag ctctgtcttt
     1921 gcttcatttt tacctgttgg gagcgtttcg tggttgtatt acgcggaaag attaatggaa
     1981 tttccgctcg gtggatttgg tgttgcgtta gcgactgtcg ttttgccgcg tttatcgcgt
     2041 catcatacca aggctgaaga tgaacatttt tcagcaacac tcgattgggg tgttcgttgg
     2101 gttatgttga ttggcctgcc cgccgcattg ggattggcgt tattagcggg acctttacta
     2161 acaactttgt ttcaatcggg tcagttcacc gatcaagatg ttatttcaag ccaaatgtgt
     2221 ttggtcgcat attcgcttgg cattatggga tttatgttgg ttaaaatatt tgcctctgcg
     2281 tattatgcca agcaagatgt taaaacaccg gttcgaatgg ctgttgtaac agtgactgca
     2341 aatcttttgt tcagtttgat tttcatgaaa tttttagcgc atacgggttt ggcgcttgcg
     2401 acatcgatgg cagcattatt aaatgcgacg cttttggggt atggtttatt taaacggcat
     2461 atttatacat tacaaccggg atggactttc ttggctctac aattactact ggcgctaagc
     2521 gcaatgggta tcgtgatacg gatgttcagt ccggatttag tagcctggtt tgcaatggat
     2581 gtaacagagc gtgttgttac actgtttgga ttaattatta cgagtggatt agcctatatc
     2641 gccactttat ttttatcagg attacgaaca aatcatatat taggaagagc aatatcagag
     2701 ggtgattcat gagtgattat aaagagacgt taaacttacc caacacagca tttcctatga
     2761 aagccaattt ggcagagcgg gaaccggctg ttttaaaacg ttggaatgaa attaagctgt
     2821 atcagcaatt agcagaagca ggtaaaaata aaattaataa agataaaact aaattattta
     2881 ttttgcctga tggtccaccg tttgcgaatg gccatattca tattggtcat gcagtgaata
     2941 aaattttaaa agattttatt ttaaaatcca aaaccctaaa tggatactat gcgcctttcg
     3001 tgcccggttg ggattgtcat ggtttaccga tcgagttaaa tgtcgaaaaa gaggttggaa
     3061 aacctggtgt taaattagat gctaaggcgt ttagagagaa atgtcgattg tatgcgatga
     3121 agcaaatcaa tcttcagcgg gatgccttta tt
//
LOCUS       DAORVT010000136         3151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_26894_length_3151_cov_3.091731, whole genome shotgun sequence.
ACCESSION   DAORVT010000136 DAORVT010000000
VERSION     DAORVT010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26894_length_3151_cov_3.091731"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PK389_03495"
     CDS             <1..202
                     /locus_tag="PK389_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HQY22803.1"
                     /translation="SDLDLCIMGTQPLSLQQLGDLREDFSESDLPFRVDIVEWTTITP
                     EFKKIIEAHCEELPPPKIFIRN"
     gene            complement(215..1612)
                     /gene="der"
                     /locus_tag="PK389_03500"
                     /pseudo
     CDS             complement(215..1612)
                     /gene="der"
                     /locus_tag="PK389_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120431.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
     gene            complement(1615..2790)
                     /gene="bamB"
                     /locus_tag="PK389_03505"
     CDS             complement(1615..2790)
                     /gene="bamB"
                     /locus_tag="PK389_03505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HQY22804.1"
                     /translation="MRIVRYTVLALGILWLSGCTTIDKQPELIKKGPLPKLSANEMIP
                     KVLWKNTQSSGSGKSDANLSLAVTGSEIVIADSKGKILALNRQTGIQKWVVSTKAEIT
                     AGPSVSEGRVLVGTDDRKVLAYELSNGTFLWQAKVTGSVMAAPVGASRMVFVHGLDGS
                     VVSLNAQNGQQLWRYSVQNPPLMLRQNSSPVVLNNHVIVGFSNGKLASLHRVDGFPEW
                     ERELAIPKGRSDIQRMVDISADPVIKDDRLYAVSYQGQIAAMIVETGLTLWERALSSS
                     SGIAVSENAVFVSDPNGVLWALNRKTGDVIWKQTALMGRELSRPAIVEGFVVVGDNDG
                     YLHWMSRNDGAFVGQNLIDSKGIKAPPVVKDNLVYVLGRSGKVTALMPIHSGVSRGN"
     gene            complement(2803..>3151)
                     /locus_tag="PK389_03510"
     CDS             complement(2803..>3151)
                     /locus_tag="PK389_03510"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY22805.1"
                     /translation="ALLLAKFSFEAHNLEATATHLNFAIQAEGPVQPIARVRLARVLI
                     DQKKPEEALALLTEKPASKGFELMFEETKGDIYLMQNDKAKARVAYQTAIEAAEPGVP
                     ITRLQLKQADLGK"
BASE COUNT          890 a          776 c          573 g          912 t
ORIGIN      
        1 ctcggattta gatttgtgta ttatgggtac acaaccctta tccctacaac aactcggtga
       61 cttacgggaa gatttttctg aatctgattt gccattccgg gttgatattg ttgaatggac
      121 caccattaca cccgaattta aaaaaataat tgaagcgcat tgtgaagaac ttccgccacc
      181 aaaaatattt atacgaaatt aagactataa ggccttattt cttctttttt gcgaatcgca
      241 ttaatcgctg gcgtttataa agctgccgcg gcgttagcgt atttctacgc cccgcaaacg
      301 ggtttgcgct ggttttaaat tcaacgcgta tgggcgtacc cactatttta aggtgctttc
      361 taaaggtatt gatcaaatag cgcttgtagc tttcgggcac atgttctgtt tgattaccgt
      421 gaataataat taagggcgga cgatgcccac cggtatgtgc atagcgtaat tttatgcgtc
      481 taccctaaaa catcgggggt ggatgttgcg taactgcaat ttccaatacc cgattcacct
      541 ctgctgttga tagctcttta atcgaagatt gaaaggcttc ttgtacagaa tcaaataaat
      601 taccaacacc actgccatgc aatgcagaaa tgtaatgtac ttttgcaaaa tctgcaaatt
      661 ttaatctgta atttaaggtc tttttaacat tttctcgatg ttcaacatct aaaccatccc
      721 acttgttaat ggctaatact aaagaacgac cggtttctaa cacaaaccct aaaagcccca
      781 gatcttgatc tgtcacactc tcgacagcat ccacaaccaa aatcaccaca tgacaatctt
      841 caatcgcctg caaagtctta accactgaaa acttttcaac cagttctgag acacgtgatc
      901 gtcgtcgcac acccgccgta tcaataatgg tatatttttt gcctgcacgc tctaagggaa
      961 tataaatgct atctcgagta gtaccgggtt gatcaaatac aatcacgcga tcttcgccta
     1021 ataagcgatt tactaaggtt gattttccca cattcggacg acctgcaatg gcaattttta
     1081 tgccactttc ttcaggattg gctatctcaa tttcacttaa cggtggaaat ggcaaaaaga
     1141 tgtgatcaat cagtgcaaca agccctcgcg cttggctagc cgctatgggg ataggatctc
     1201 ccaatcctaa tgcataaaaa tcagctaaag caatgtccgg atttaaacca tccgttttat
     1261 ttacaacaca ataaacagcc ttttctttaa cacgtagttg ttgcgcaatg gttttatccg
     1321 ctgccattaa cccagtgcgc ccgtcaacta taaataaaac aatgtctgct tcgacgaccg
     1381 cttgccaaga ctgctcttcc gtcagggtat ctaattccgc ttcttgtgac cctaagcccc
     1441 cagtgtctat agcaataaag ggtctttccc ctattttgcc ttcgccatat tgacgatctc
     1501 gggtaacccc cggtagatca accactaaag cctctcgact ttttgttaat tgattaaata
     1561 atgtggactt gcctacatta ggacgtccaa caatcgctat aacaggaagc atgtttaatt
     1621 cccccgagag acacccgagt gaatcggcat taaagctgtc acttttccac ttcggcctaa
     1681 cacataaacg agattatctt ttacaactgg aggcgcttta atgcctttac tgtcaataag
     1741 attttgccct acaaaagcac cgtcatttcg cgacatccaa tggagataac catcattatc
     1801 gccaacaacc acaaagccct ctacgatggc cggtctagac aactctctgc ccattaatgc
     1861 cgtttgtttc caaataacat cgcctgtttt tcgattcagt gcccaaagaa caccattggg
     1921 atccgacaca aagacagcat tttccgaaac cgcaatacct gaagaagaag ataaagcacg
     1981 ttcccaaagg gttaagcctg tttcaacgat catagcggca atttgtccct gatagctgac
     2041 agcgtacagt cgatcatctt taataacggg atcagcactg atatccacca ttctttggat
     2101 atccgaacga cccttgggaa tcgccaattc tcgctcccat tcgggaaacc catccacgcg
     2161 atgcaatgag gctaatttac cattcgagaa acccacaata acgtgattat ttaaaacgac
     2221 aggactacta ttctgtctta gcatcaaggg agggttttga accgagtaac gccatagctg
     2281 ttgtccattt tgcgcattca gagaaacgac actgccatcc aaaccatgaa caaataccat
     2341 gcgagaagca ccgacaggtg cagccatcac agaacctgtt acctttgctt gccataaaaa
     2401 agttccgttc gacaactcat acgccaaaac ttttctatca tctgttccaa ctaaaacgcg
     2461 tccttcagaa accgaaggtc ccgcggtaat ttcggcttta gtagacacaa cccatttttg
     2521 aatacctgtt tgacgattca acgccaaaat ttttccttta ctatccgcaa taacaatttc
     2581 agatcccgta acggctaaac ttaagtttgc gtcggacttg ccactaccag aactctgtgt
     2641 atttttccaa agtactttag ggatcatttc atttgctgat aatttgggta atggaccctt
     2701 cttaatcagt tcgggttgtt tatcaatcgt cgtacagccc gaaagccata aaatccccaa
     2761 tgccaatacc gtataacgca ctatgcgcat attaaattcc tcttatttgc ccaaatccgc
     2821 ttgttttaat tgcaagcgtg tgatgggaac gcctggttct gccgcttcaa tagccgtttg
     2881 ataagcgact cgtgcttttg ctttatcatt ttgcatcaga taaatatcgc ctttggtttc
     2941 ttcaaacatc agctcaaatc ccttggacgc cggcttttct gtgagaagcg ctaaggcttc
     3001 ttctggtttc ttttgatcaa ttaaaacacg ggccaatcgc actctcgcaa tcggttgcac
     3061 tggcccttct gcttggattg caaagttcaa atgtgttgct gttgcttcca ggttatgggc
     3121 ttcaaaagaa aactttgcta acaacaaagc t
//
LOCUS       DAORVT010000137         3148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_26937_length_3148_cov_3.599095, whole genome shotgun sequence.
ACCESSION   DAORVT010000137 DAORVT010000000
VERSION     DAORVT010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26937_length_3148_cov_3.599095"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..513)
                     /locus_tag="PK389_03515"
     CDS             complement(97..513)
                     /locus_tag="PK389_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HQY22806.1"
                     /translation="MSRQLSQIVQHWGYIAPTVSYPKNDNLFDKLVNLQDELLTIVGS
                     DEKHALIGLLDIISHFIEEYELNRCGIEQKQPTGIDALKFLMEAHHLKQSDLPEIGSQ
                     GVVSEILNEKRILNVRQIRRLSKRFNVNPSTFIDKE"
     gene            complement(515..811)
                     /locus_tag="PK389_03520"
     CDS             complement(515..811)
                     /locus_tag="PK389_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003187960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HQY22807.1"
                     /translation="MHIITQKRIWDAKVKYPEVSSALDGWYRIMKKNQFFGFSAMKRS
                     FNSLDKVNDFYIFDIGGNKLRLIVNIHFNRQKVYVRYVLTHREYENGTWKQKGR"
     gene            complement(972..1805)
                     /locus_tag="PK389_03525"
     CDS             complement(972..1805)
                     /locus_tag="PK389_03525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HQY22808.1"
                     /translation="MRSTTKTTLFDLNPFYGLIAVSGSEAATFLQGQITADIRTITST
                     QPGFSAYCNAKGRIRALFRIFFHHECYFLQLPVGVLPSALATLKKYARFSKVKLEDVS
                     EQWQRIGICLTHADLKTIDFLDLKDIILLPLPSPEFYKRFELIGLPNIVKPLWDLFLA
                     SENSVISDFDAWKYLDIQAGIPEIWPETIEQFLPHSLNLPALGAVSFNKGCYCGQEIV
                     ARMEYRANVKHKLFNTTLPDANSIPLPSDKLYAENGSDEIIGTVVTASRTEILGVKSK
                     Q"
     gene            1868..2149
                     /locus_tag="PK389_03530"
     CDS             1868..2149
                     /locus_tag="PK389_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011758988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HQY22809.1"
                     /translation="MESAPMKTTEIPSLPSTPSIRWQCRRGMLELDMLLLPFFDTVYD
                     TLGVEQKQAFIDLLAFPDQEIVRFLMGRQIPDSLALQSIVQQICGFKLG"
     gene            2155..2565
                     /locus_tag="PK389_03535"
     CDS             2155..2565
                     /locus_tag="PK389_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22810.1"
                     /translation="MERIEFVLGRSRLLFLFLGAVYSCAVLVLVNCPLSIILKIAGTT
                     LLLWYFWQLVNLHVKRISKKSVVCIWQDPTGRWGCLTHQKKAAIGELKSDSFKSAVVL
                     VLRFRFKTGIRNVIIPVDALNKLEYQQLSARLSL"
     gene            2703..>3148
                     /locus_tag="PK389_03540"
     CDS             2703..>3148
                     /locus_tag="PK389_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQY22811.1"
                     /translation="MYNNRDDLQLIEAAKQGDSRAFDALVLRYQSRVFKVVARFVKDP
                     SESLDVCQEVFIKVYRALDKFRSDSSFYTWIYRISVNTAKNFIISRGHRLPDIDIEMI
                     DMDRFVMRNSLKEFGTPERLMMRDEIEGVVYDTIERMPNDLRTAIK"
BASE COUNT          941 a          568 c          620 g         1019 t
ORIGIN      
        1 ttaacggatt ctccatcgat tcgagatgtt ttgttgtttc cgcatatgaa ggcgcaaggt
       61 gttaaagaag gggttaaagt ttgattctgt ttttttctat tctttatcaa taaaagtgct
      121 aggattaaca ttaaaccgtt tagataatcg tctaatttgt ctgacattca gaattcgttt
      181 ttcgtttaat atttctgaaa caacgccttg gctgccaatt tcaggtaaat cggattgctt
      241 taagtggtgg gcttccatca gaaattttaa ggcatctatt ccagtgggct gtttttgttc
      301 aatgccacat cgattcagct catattcttc aataaagtga ctgataatat cgagtaagcc
      361 aataagcgcg tgtttctcat ccgaaccaac gatagttaat aattcatctt gcagattaac
      421 gagtttatcg aacaagttat catttttagg gtaactaaca gtaggcgcaa tatagcccca
      481 atgctgaaca atctgagaaa gttgtctact catcttacct tcctttttgt ttccaagtac
      541 cgttttcata ttctctatgc gttaacacat accggacata aactttctgc ctattaaagt
      601 gaatattgac aatcagccga agcttgttac caccgatatc aaaaatgtag aaatcattca
      661 ccttatctag gctgttaaag cttcgtttca tcgccgaaaa accaaaaaac tgatttttct
      721 tcataatcct gtaccagcca tctaatgcgc tggagacttc tgggtacttt actttagcat
      781 cccatatcct cttttgagta ataatgtgca taattcaccc attaagcata tatctcaaaa
      841 tgagataagt caataaaaat ttcgttctag tttattgcgt ttagggaggc tgcaaatttt
      901 ctgtttccct ttaattataa taagtgttct gttttcaaaa ctaaatccgt caaataacct
      961 agaatttaca tctattgctt agacttaaca cccaatattt cagttcgact tgctgtaaca
     1021 accgttccaa tgatttcatc actaccattt tcggcataaa gtttatcgct cggtaaagga
     1081 atgctatttg catccggtaa agttgtatta aacaacttgt gtttaacatt ggcgcgatat
     1141 tccattctgg cgactatttc ttgtccgcaa tagcagccct tattaaaact cacggcacct
     1201 aatgcaggta agtttaaact gtgcggcaga aattgttcga tggtttctgg ccaaatttcg
     1261 gggatgcccg cttgtatatc taaatatttc cacgcatcaa aatcagaaat gaccgaattt
     1321 tcagatgcca gaaatagatc ccataagggt ttaacaatat taggcaagcc aatcaattca
     1381 aagcgtttat agaattcagg actgggtagt gggagcagta taatgtcttt cagatccaga
     1441 aaatcgatag tttttagatc agcgtgtgtt aaacagattc caatccgctg ccactgctcg
     1501 ctcacatctt ccagtttaac tttagaaaat cgcgcatatt tttttaatgt ggctaaagcg
     1561 cttggcaaaa caccaacagg cagctgcaaa aaataacatt cgtgatgaaa aaaaatgcga
     1621 aataaggcgc ggatgcgacc tttagcgtta caataagccg aaaacccagg ctgcgtagat
     1681 gtaatggttc taatatcggc cgttatttga ccttgcagaa acgttgctgc ctcggatcct
     1741 gatacagcaa taagtccata gaatggattc agatcgaaca gcgttgtttt agttgtggat
     1801 ctcatgaata ccttgaaata agctaaaaaa attgataata tttaaatcat atcatatttg
     1861 gatacgaatg gaatcagcac cgatgaaaac gaccgagata ccttcattac ccagtactcc
     1921 cagcattaga tggcaatgtc gtcgcgggat gttagaatta gatatgctgc tgttgccttt
     1981 ttttgatacg gtttacgata cattgggcgt agaacaaaaa caggcgttta ttgatttgtt
     2041 ggcttttccg gatcaagaaa tagttaggtt tttaatggga cgacaaatac ccgatagcct
     2101 tgctttgcaa tctatagtac aacagatatg tggttttaaa ctagggtaag gtatttggaa
     2161 agaatcgaat ttgtactcgg gcgttcgagg ttgttatttc tttttctagg cgcggtgtat
     2221 tcttgtgctg ttcttgtttt agtaaattgt ccattgtcga ttatactcaa aatagcagga
     2281 accacactgt tattatggta tttttggcag ctggtgaatc tgcacgtaaa aagaatatct
     2341 aaaaaatcag ttgtttgcat ttggcaagat cccactggac ggtgggggtg tcttacccat
     2401 cagaaaaaag cggcgattgg tgagcttaaa agcgatagtt ttaagagtgc cgttgtgttg
     2461 gtcttgcgct ttcgttttaa aacgggtata cgaaatgtga ttattcctgt agatgcactc
     2521 aataaattgg aatatcaaca gttgtctgca cgccttagcc tataatttta aaaataaagt
     2581 aatcaagata ttacccatga tattacacat tgaaataaga tttttatttc actagactaa
     2641 aaattgaacc aagacatatt gccaaaggga ctggtaatcg cgaataacgg aggctggtcc
     2701 acatgtacaa taatagagat gatttgcaat taatcgaggc tgctaaacaa ggtgattcta
     2761 gagcatttga tgctttagtg ctgcgctatc aaagtcgtgt ttttaaggtt gttgcgcggt
     2821 ttgttaaaga tccgagtgag tccttagatg tttgtcagga agtttttatt aaagtttatc
     2881 gcgcgttaga taaattccga tctgatagtt cattttatac ttggatatat cggatttcag
     2941 tcaacacggc taaaaatttt attatttcgc gagggcatcg gttaccggat attgatattg
     3001 aaatgatcga catggataga tttgtgatgc gcaacagtct taaagaattt ggtacacccg
     3061 aacgtttaat gatgcgtgat gaaattgaag gcgtcgttta tgatacgatt gaacgcatgc
     3121 cgaatgatct tcgtaccgct attaagtt
//
LOCUS       DAORVT010000138         3131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_27238_length_3131_cov_2.344278, whole genome shotgun sequence.
ACCESSION   DAORVT010000138 DAORVT010000000
VERSION     DAORVT010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27238_length_3131_cov_2.344278"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="PK389_03545"
     CDS             <1..1048
                     /locus_tag="PK389_03545"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQY22812.1"
                     /translation="NRARTKSNNLNRSSKNKILSLVLAFLVSFITCLDISIAEPLTQK
                     TIGISQIVEHPALDSVRESMLAALKAEGYEAGKNLKVIYDNAQGNIVTSTQIANKLMS
                     SKINLAVGISTPSAQTLFYEAKKHGSKIPIVFSAVSDPKAAGLESCNDFPITGVTDTA
                     HLEGLLELIKSLMPQLKTLGLMYNAAETNSVSTIGRLKILLKKNDIRFVEVTVNKTQD
                     VADATKSLIGKVDALYFPQDNTLVSAIQTIVNIASQSSPTLPIILPIFTSDPLLVQSG
                     VLAAVGYDYQDIGRETGIIVAKILKGENPKNMPTHNPSTLKAFINSSLAKRLGLAIPS
                     KLAFSEIQVTKDRP"
     gene            1183..1482
                     /locus_tag="PK389_03550"
     CDS             1183..1482
                     /locus_tag="PK389_03550"
                     /inference="COORDINATES: protein motif:HMM:NF025691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HQY22813.1"
                     /translation="MSTQNEPRPQTDSHTREKLLKALYLVLFYFVGYVVWILTVAISV
                     FQFFYSIFLKHPNQNLLDFGKSLNQYLYEIVRFISFNTDTKPYPFSPWPSSDKIN"
     gene            1522..2385
                     /gene="hpnD"
                     /locus_tag="PK389_03555"
     CDS             1522..2385
                     /gene="hpnD"
                     /locus_tag="PK389_03555"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HQY22814.1"
                     /translation="MDDSDKYCREKTAKSGFSFYYSFLFLPTEQKKAIMAVYAFCREV
                     DDIVDECTDKEIAQKKLAWWHTEIDRVFTDQPQHPVGKALAQIKDRFSLKKHLFKEIL
                     QGMSMDLSYQGYQTFEDLRLYCHCVASAPGLLAAEIFGYTNPNTLEYAKNLGIAFQLV
                     NIIRDVGEDANRCRIYLPEDELAKFSLTPQDILEKKYTQNFQDLMAYQATRARSYYEK
                     ALLTLPEADKYDQRCGLIMAAIYFVLLSEIEKLHFKILDQRVSLTPLRKLWIAWKTQR
                     TLKPSKKNTHV"
     gene            2378..>3131
                     /locus_tag="PK389_03560"
     CDS             2378..>3131
                     /locus_tag="PK389_03560"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY22815.1"
                     /translation="MFKLSEEKMASTSASTLVVGAGWAGLAAALTLTRQGHQVVLLEA
                     APQAGGRARGIPFGSYTVDNGQHLFMGAYTETLMLLKWLNISEDAVFDRKPFEWFMLN
                     QKNTQKSIHLTAPSSVSLLKQLLSFSKIQGFSLKERLRAIAFYRTIYHKNFHLSQDIS
                     VYELLLRFKQPISLIEKLWGPMALAALSTPIHQASAQVFVKILRDIFKQNIKQGNTKS
                     PSDWLFSKVDLSTLLPNLIIKYIKEKGGSIFYN"
BASE COUNT         1019 a          676 c          556 g          880 t
ORIGIN      
        1 taacagagca agaacaaagt caaacaactt aaaccgatct tctaaaaata agatcctgtc
       61 tcttgtcctc gccttccttg taagcttcat cacctgccta gatatctcaa tcgccgagcc
      121 tcttactcaa aaaacaatcg gcattagcca aatcgtagag caccctgcgc tcgactctgt
      181 acgcgaaagt atgctggccg ctttaaaagc agaaggatac gaagcgggaa aaaatttaaa
      241 agtgatatac gataatgcac aaggtaatat tgttacttca acacaaatcg ccaataaatt
      301 aatgagttcg aagattaatc tggcggttgg catttctaca ccctctgcac aaaccttgtt
      361 ctacgaagcc aaaaaacacg gctcaaaaat tcctatcgta tttagcgcag tgagcgatcc
      421 taaagcagcg ggcttagaat cttgtaacga ctttcctata acgggtgtta cagatacagc
      481 ccatctagag gggctgcttg aactgataaa atctttaatg ccacagttaa aaacactggg
      541 attaatgtat aatgcggcag aaacaaattc tgtttcgacc attggccgtt taaaaatatt
      601 attaaagaaa aatgacattc gttttgttga agtcactgtt aataaaacgc aagatgttgc
      661 agatgcgaca aaaagtttga tcggcaaagt ggatgcactc tactttccac aggataatac
      721 acttgtatct gctatccaaa ccattgttaa cattgcctca caatcgtcac cgacacttcc
      781 gatcattctg cctattttca ccagtgatcc tttgttggtt caaagcggtg tcttagccgc
      841 agtggggtat gattatcagg acattgggcg cgaaactggc ataatagtcg cgaaaattct
      901 aaagggagaa aacccaaaaa atatgccaac acacaatccc agtacgctta aagcgtttat
      961 taacagttcg cttgcaaaac gcttaggatt agcgattcct tcaaaattag cattttctga
     1021 aatccaagtt acaaaggata gaccttaact ttatacttgc cctaatcttg tgctaaacag
     1081 gctattttag agcaattctt tctggaaatt cacacttgaa tgcactaagc taagataata
     1141 tgcaagcaca tttatgaata actacaaaac aggagtagtt ctatgtcaac ccaaaacgaa
     1201 ccacgcccac aaacggattc gcataccaga gaaaaattgc taaaagcgtt atatctcgtt
     1261 ttattctatt ttgttggtta tgtcgtttgg attttaaccg tcgccatttc agtttttcaa
     1321 tttttctaca gtatttttct aaaacacccg aatcaaaatt tattggattt tggaaaaagt
     1381 ctgaatcagt atctgtatga gatagtacgc tttatctcgt ttaatacaga cacaaagcct
     1441 tacccatttt caccctggcc aagcagtgat aaaatcaact aacaccttgc aaatattgtc
     1501 gacccttgtt agggtaagac gatggatgat tcagacaaat attgccgaga aaaaaccgcc
     1561 aaaagtggtt ttagcttcta ttacagcttt ctctttttac ctactgagca aaaaaaagcc
     1621 attatggcgg tctatgcctt ttgcagagaa gtcgatgata tagtggatga atgtacagac
     1681 aaagaaattg cacaaaaaaa acttgcttgg tggcatacgg aaatcgaccg tgtttttaca
     1741 gatcaaccgc aacaccccgt cggaaaagca ctcgcacaga ttaaagatcg cttttcgctt
     1801 aaaaaacatt tattcaaaga aatcttgcag ggtatgagca tggatctctc ttatcaggga
     1861 taccaaactt ttgaagattt gcgactgtat tgccattgtg tcgccagtgc acccgggcta
     1921 ttagccgccg aaatttttgg atatacaaat cccaacacgc tagaatacgc caaaaatcta
     1981 gggatcgctt ttcaattagt caatattatt cgagacgtag gcgaagatgc aaatcgctgc
     2041 agaatttatt tacccgaaga tgaactggcc aagttttcac tcacacccca agatatttta
     2101 gaaaaaaaat acacccaaaa ttttcaagat ttaatggcgt accaagcaac acgcgcgcga
     2161 tcctattatg aaaaagcttt actcacctta cctgaagcgg ataaatatga tcagcggtgt
     2221 ggactgatta tggctgcaat ctattttgta cttcttagtg aaattgaaaa attacatttt
     2281 aaaattttag atcaacgcgt cagcttaaca cccttacgta agctttggat tgcatggaaa
     2341 acccaaagaa ccctgaagcc ctctaaaaaa aatacacatg tttaaattat ctgaagaaaa
     2401 aatggcgtcg acctcggcat ctacgctcgt tgttggcgcc ggctgggcag gactcgccgc
     2461 agcactcaca ctcacccgac aagggcatca agtcgtttta ttagaagcag ccccacaagc
     2521 aggcggccgg gcacgcggca ttccatttgg atcttatacg gttgataatg gacaacattt
     2581 attcatgggc gcttacacag aaaccttaat gctcttaaaa tggttaaata tttcagaaga
     2641 tgctgtgttc gatcgaaaac cctttgaatg gttcatgctt aatcaaaaaa acactcaaaa
     2701 atctattcat ctaacagcac catcgtctgt atcactatta aaacagctac tcagtttttc
     2761 aaaaatccag ggcttttcat tgaaagaacg tttaagagca atcgcgtttt accgtacaat
     2821 ttatcataaa aactttcatc tctctcaaga tatctctgta tacgaactat tgctgcgttt
     2881 taagcaaccg atttctctca tcgaaaaatt atggggaccc atggcacttg ctgctttaag
     2941 cacgcctatt catcaagctt ccgcacaagt attcgtgaaa atcctacgcg acatcttcaa
     3001 gcaaaacatt aaacagggta atacgaaatc tccttccgat tggttattct ctaaagttga
     3061 tctcagcaca ttgttgccca atctaatcat caaatatatt aaagaaaaag gtggttccat
     3121 tttttataat c
//
LOCUS       DAORVT010000139         3028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_28893_length_3028_cov_3.852001, whole genome shotgun sequence.
ACCESSION   DAORVT010000139 DAORVT010000000
VERSION     DAORVT010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28893_length_3028_cov_3.852001"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..301
                     /locus_tag="PK389_03565"
     CDS             20..301
                     /locus_tag="PK389_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008222449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HQY22816.1"
                     /translation="MHYCLDQGVHYTIRKTITLTDKQDQWIKTQIAAGEFANDSEYIR
                     DLVRRDQEQNAQYMALKQAIQEGLDSGVSNKTVGEIWEEAEQRSKNRRG"
     gene            663..926
                     /locus_tag="PK389_03570"
     CDS             663..926
                     /locus_tag="PK389_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22817.1"
                     /translation="MLSIAPNERRLGLLFARIRILGAGPDPIFCEIWLVLCDLHNQMI
                     HEYMEDLDKLTEALYKGHEYVSTLTGDATRILVDLEARGWIAS"
     gene            1069..1365
                     /locus_tag="PK389_03575"
     CDS             1069..1365
                     /locus_tag="PK389_03575"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HQY22818.1"
                     /translation="MRLDTVQRNALKTALQHLQTSDQVFLFGSRVDDAKRGGDIDLLI
                     FSEQPEFELTQKISRDFFKQCEEKIDVLVINPKRMTKEQSIFVRSIHKEPLVLK"
     gene            1362..1793
                     /locus_tag="PK389_03580"
     CDS             1362..1793
                     /locus_tag="PK389_03580"
                     /inference="COORDINATES: protein motif:HMM:NF020360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HQY22819.1"
                     /translation="MMTVREKQLEIARENYSKVYQLLRASVNKTTDFDSERNYSADEL
                     EPYDALSDRFIRTVELAIKFFRTYEYYMRVEQSQTLRDGLHQMEKLGAISSLDIWLDM
                     RDIRNRIVHDYAPAKIAQMFHLIRNDFYHELTRLDRFVVQV"
     gene            2228..2629
                     /locus_tag="PK389_03585"
     CDS             2228..2629
                     /locus_tag="PK389_03585"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HQY22820.1"
                     /translation="MTHEPVGNSLKILQKTLSLRDDIALSFLFGSKARGDSHEQSDWD
                     IAILFKDNTNGWENLGKQEEIRRLLSRALATNDDNIDLVDLYRGGLSINATVVDEGIP
                     LTGQNSLALAIFYQRIWANLEDYYWNLDHVS"
     gene            2619..3026
                     /locus_tag="PK389_03590"
     CDS             2619..3026
                     /locus_tag="PK389_03590"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HQY22821.1"
                     /translation="MYLELYQKETEKLAERNGKLLKELHNKLMQTADLSLIEEQAMLH
                     ALQIIIENAIGKARHLLKAKNEKVPISAYDLFEQLKSLDVIDDAALQEWKKIIGLRNT
                     IVHEYMKIDIPLVKIIVTNVSKFILTQEYPWKA"
BASE COUNT          976 a          514 c          702 g          836 t
ORIGIN      
        1 ggttgccctc ttaaactcga tgcattattg tcttgaccaa ggggtccact ataccatacg
       61 aaaaacaatt acgttaactg ataagcaaga tcaatggatt aaaacgcaaa ttgctgctgg
      121 cgagtttgcc aatgacagcg aatatattcg tgatttggtg cgccgtgatc aggaacaaaa
      181 tgctcaatat atggctctga aacaggctat tcaggaagga ttggatagtg gcgtaagtaa
      241 taaaactgta ggtgagattt gggaagaagc cgagcagcgt tctaaaaata gacgtgggta
      301 aattctactt aacgaatcgc gcttttactg acctttctta aattgcagat atcctgatcg
      361 ttcgggtttt acatcaacgg atggatttta agcgacgcat agcgtcttct ataaaagagc
      421 tgtgacataa tcaaacattt ttcatagcga ttatccttga gtgtcagaag gaaggcggca
      481 agtcgcgatc caagaaaatt gattgtatgc tgcgaactat gacaagacca cttcgtttgg
      541 gatttcagaa tacgctttat aacgtaacat cgcgagggga tcgcagcgca acacaaaggc
      601 cggaaaatgg cgattattgc caactataaa caagatgcat acagtcaacg agaaatctgg
      661 gggtgctatc aattgcgccc aacgaacggc ggttggggtt attgtttgca agaataagaa
      721 ttcttggtgc tgggcctgac cctatttttt gcgagatatg gttggtatta tgtgatttgc
      781 acaatcagat gatccatgaa tacatggaag acctggacaa actgaccgaa gcgctataca
      841 aagggcatga gtatgtatcc acactaacag gggatgccac cagaatattg gtggatttgg
      901 aagcaagagg ctggatagcc tcataaaaga tgtggcaatt gtggtttcaa aaccattgcg
      961 ttcagacttt ttgatgagaa aacatgcaga attgaaaatc cacttgcgta aaatcaagtc
     1021 cctttaccat ataaattttg cagtggttat tggaaaaata attgaatcat gcgtttagat
     1081 actgttcaaa gaaatgcact aaaaaccgca ttgcaacatc tgcaaacaag tgaccaagta
     1141 tttctgtttg gttctagagt ggatgatgcc aagcgggggg gggatattga cctgctcatt
     1201 ttttcagaac agcctgaatt tgagttgacg cagaaaatct cccgagattt ttttaaacaa
     1261 tgcgaagaaa aaatagacgt gctggtaatt aatccaaagc ggatgacaaa agagcaatcc
     1321 attttcgtgc gatccattca taaagagccg ttggtgctga aatgatgaca gttagagaaa
     1381 agcaattaga aattgctcgg gaaaattata gtaaggttta tcaactatta cgtgctagcg
     1441 ttaataaaac aacggatttt gatagtgaaa gaaattactc tgcggatgag ctggagcctt
     1501 atgatgcctt aagtgatcgt tttatccgca cggtcgaact ggcgatcaag ttttttagaa
     1561 cttatgaata ctatatgagg gttgagcaaa gccagacctt aagagacggt ctgcatcaga
     1621 tggaaaagct gggtgcgatt tcgtctttgg acatttggtt ggatatgcga gatattcgca
     1681 atcgcatcgt acatgattat gcgccggcga aaatagcgca aatgtttcat ctaattcgaa
     1741 acgactttta tcatgaatta acacggttgg atcgttttgt tgtacaagtt tgatgaaaag
     1801 aataactatt gggcgtcgct aaaaattcaa agtttaaaac agaacgaggc gcgagcaaaa
     1861 aatgttgacg cagcatataa ttgatatgta aggaaatatt tttttcgagc aacaaagtgt
     1921 tgtgacaaaa tctgtttttt tagagatgcc ctattattag gttggagtat gttggtctac
     1981 ctttcacgag attgtgaaga ttaatgttgt ctgacgcctt ttattaaacg actaaatttt
     2041 atgcaatagc aatgtattga gtcatttgaa ggtgtacaga ataatttagc agatatgcgt
     2101 gcatctgcaa cgaaactagt gcgtgatttt tgtggttgat gggtggtggt ttatagagca
     2161 gatagtttaa gtttcttgaa ataataatgc cagaagactg taaatggaga ataaaagaac
     2221 gaagagtatg actcatgaac cagtaggtaa ttctttgaag attttgcaga aaaccttaag
     2281 tttgcgtgac gacatagccc tttcatttct gtttggcagc aaagcacgag gtgatagcca
     2341 tgaacagagt gactgggata tcgccatctt attcaaagac aacaccaatg gctgggaaaa
     2401 tttaggtaaa caggaagaga tcaggcgcct tctctcacgg gccctggcaa caaacgacga
     2461 caacattgat cttgttgatt tgtatcgtgg cggactgagt attaatgcca cagtcgttga
     2521 tgaaggaatc cccttgactg ggcagaacag tttggcattg gcaatttttt accaaagaat
     2581 atgggccaat ttggaagact attattggaa ccttgatcat gtatcttgag ctgtatcaga
     2641 aggaaacaga aaagcttgcg gaaagaaatg gcaagctgct aaaagaattg cacaacaaac
     2701 tgatgcagac ggcagatctc tctctaatag aagagcaagc catgcttcat gcgctacaga
     2761 ttattattga aaacgcaatt ggtaaggcca gacacctatt aaaagcaaag aatgaaaaag
     2821 tgccgatcag tgcttacgac ttgtttgaac agctgaagag cctcgatgtg atagatgatg
     2881 cagccttgca ggaatggaaa aaaattattg gcctacgaaa taccattgtg catgaatata
     2941 tgaagatcga catcccactt gttaaaatca tagtaactaa cgtttcgaag ttcattttaa
     3001 cccaagaata cccttggaag gcttgatt
//
LOCUS       DAORVT010000140         3006 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_29270_length_3006_cov_4.928160, whole genome shotgun sequence.
ACCESSION   DAORVT010000140 DAORVT010000000
VERSION     DAORVT010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29270_length_3006_cov_4.928160"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /gene="rplP"
                     /locus_tag="PK389_03595"
     CDS             complement(<1..245)
                     /gene="rplP"
                     /locus_tag="PK389_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HQY22822.1"
                     /translation="MLQPARTKYRKQQKGRNTGIATRGAKVSFGEYGLKAIGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRIFPDKPISHKPAEV"
     gene            complement(457..1083)
                     /gene="rpsC"
                     /locus_tag="PK389_03600"
                     /pseudo
     CDS             complement(457..1083)
                     /gene="rpsC"
                     /locus_tag="PK389_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185240.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
     gene            complement(1085..1447)
                     /gene="rplV"
                     /locus_tag="PK389_03605"
     CDS             complement(1085..1447)
                     /gene="rplV"
                     /locus_tag="PK389_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HQY22823.1"
                     /translation="MMETTAKLRGVRLSAQKGRLVADQIRGLSVDKALNILSFSPKKG
                     AGIIKKLLESAIANAEHNDGADIDELKISTILVDRGALMKRTSARAKGRGNRIVKPTC
                     HILLTVDDSIDNNKKGSN"
     gene            complement(1454..1735)
                     /gene="rpsS"
                     /locus_tag="PK389_03610"
     CDS             complement(1454..1735)
                     /gene="rpsS"
                     /locus_tag="PK389_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HQY22824.1"
                     /translation="MARSVKKGPFVDVHLLEKIEKVRETNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGKQHIPIFVTENMVGHKFGEFSLTRTFKGHAGDKKAVTKR"
     gene            complement(1743..2576)
                     /gene="rplB"
                     /locus_tag="PK389_03615"
     CDS             complement(1743..2576)
                     /gene="rplB"
                     /locus_tag="PK389_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HQY22825.1"
                     /translation="MALVKLRPTSPGRRSVVKLVNKELHKGSPHKSLLEKQSNTAGRN
                     HHGRITTRHRGGGHKQHYRMIDFKRDKLDIIATVERIEYDPNRTANIALVCYQDGERR
                     YIIAPKGLSVGAQISSGKNAQIKPGDALPLRNIPMGTVIHCIELLPGKGAQLARAAGT
                     SVQLQARDGNYAQLKLRSGEIRKVHIDCLATIGEVGNSEHNLRSIGKAGAVRWRGIRP
                     TVRGVAMNPIDHPHGGGEGRTSAGRHPVSPWGTPAKGYRTRNNKRTEIMIARHRYSKK
                     G"
     gene            complement(2576..2917)
                     /gene="rplW"
                     /locus_tag="PK389_03620"
     CDS             complement(2576..2917)
                     /gene="rplW"
                     /locus_tag="PK389_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HQY22826.1"
                     /translation="MSNVNLNLKHERLLKIILAPYVSEKATFLGEKNNQAVFRVANDA
                     TKKEIKSAIELLWKEQKIEISTVHTINVKGKQKRFGRFVGKRSDWKKAIVSIKDDKEL
                     SFTNLTNSEAK"
BASE COUNT          753 a          691 c          458 g         1104 t
ORIGIN      
        1 ctaacttcag caggcttatg agagattggc ttatcaggaa aaattctaat ccagattctc
       61 ccccctcttt ttatatgtct tgtcatagct cttctcgcag cttcaatttg tctggctgtt
      121 aaacgacctc taccaattgc tttaagacca tactcaccaa aactaacttt tgctccacgt
      181 gtagcaattc ctgtatttct gcctttttgc tgcttacgat atttggttct agctggctga
      241 agcatcttta tctccagatt ttacggatat gtcagtctta ccggcattgg tgctcataat
      301 ttcaggctca ttctcagcat taggaattga atcgatacct tttttcagcg cagaattcga
      361 caacttactc atagtacttg tggttcttct tgtgttcttt tttttatcat tttccgtagc
      421 tgcagcggat gttaggttta cattattctc tgcatcacct agtaattcac ctttgaatat
      481 ccaaaccttg atgcctataa ttccataagt agtttttgct tctgatgtac cataatctaa
      541 gtcagctctt agtgtatgga gcggtactct gccttctcga taccattcag ttcgagctat
      601 ttcaatacca tttaatcgac cagaactcat tatcttaata ccctgagctc ccaaacgcat
      661 cgcattttgc attgcacgtt tcatagctcg tcgaaacata atcctttttt ctaattgctg
      721 tgcaatgtta tctgctatta attgtgcatc taactctggt ttacgaattt cctcgatatt
      781 cacatgcacg ggcacaccca ttcgtttttg caattcggcg cgtaatatct ctatatcctc
      841 acctttttta ccaataacga ctcctggccg cgaactaaat atcgtaattc gagcattctt
      901 tgatggtctt tcaattaaaa ctctgccaac agatgcattt ttaagctttt cacttagaaa
      961 agttctaact tttatatcct cattaagcat tgtggcaaaa ttaccactgt tcgcatacca
     1021 tcttgatagc caatttcttt gcactgataa gcggaaccca gtagggtgta ttttctgccc
     1081 catatcaatt actacctttt ttattattat cgatactatc atcaactgtc agcaaaatat
     1141 gacaggttgg tttaacaatt cgattacccc gtccttttgc tcttgcacta gttcttttca
     1201 tcaatgcacc tcgatctacg agaattgttg agatttttaa ttcatctata tctgcaccat
     1261 cattatgctc tgcattagct atagcagatt ctaaaagttt ttttataatg ccagcgcctt
     1321 tcttgggact aaaggataaa atattcaatg ccttgtccac agacaatccc ctaatttggt
     1381 cagccactaa acgccctttt tgtgctgaca atcgtacacc tcgtagtttc gcagtagttt
     1441 ccattataat tccctatcgc ttggttacag cttttttatc acctgcatga cctttaaatg
     1501 tacgtgttag tgaaaattca ccaaacttat gtcctaccat attttctgtc acaaaaattg
     1561 gaatatgctg ttttccattg tgaaccgcaa ttgtcaatcc aataaagtca ggcaagatcg
     1621 tagaccttct ggaccaagtt ttgattggtc ttttatcatt agtttcgcga actttttcta
     1681 ttttttctaa taagtgaacg tctacaaaag gtcccttttt tactgaacga gccatttata
     1741 tattatcctt tctttgaata tctatgtctt gctatcatta tttcggttct tttattgttc
     1801 ctagttctat atccttttgc gggcgttccc catggactta ctggatgacg acctgctgat
     1861 gttctacctt ctcctccacc atgaggatga tcaattggat tcattgctac gccacgtacg
     1921 gtaggtctta ttccacgcca tcgaacagcg ccagccttac caattgatct taaattgtgt
     1981 tcagaatttc ccacttcacc gatcgttgct aagcaatcga tatgaacttt ccgaatttcc
     2041 ccggatcgta atttcaattg tgcataatta ccatcccttg cctgcaattg taccgatgta
     2101 cctgcagcgc gagctaattg agctcctttt cctggcaata attctataca atgtatcact
     2161 gtacccatcg gtatatttct caatgggagc gcatcaccgg gttttatttg agcattcttt
     2221 ccactggaaa tctgtgcgcc tacactcaat cctttgggag caatgatata tctccgctct
     2281 ccatcttgat aacaaactaa agcaatattt gcagttctat tgggatcata ttctatgcgc
     2341 tctaccgttg ctattatatc aagcttatca cgtttaaaat caatcattcg atagtgttgt
     2401 ttatgcccgc caccacgatg tcttgttgtg attcgtccat gatgattcct accagcagta
     2461 ttagattgct tttctaacaa acttttatgt ggtgaacctt tatgcagttc tttattaaca
     2521 agcttaacta ctgatctgcg tcctggcgat gttggtctta attttactaa tgccatcatt
     2581 tagcctctga atttgttaaa tttgtaaaac ttaattcttt atcgtctttg atgctgacta
     2641 ttgctttttt ccaatcgctt cttttcccaa cgaaacgtcc aaatcgcttt tgtttccctt
     2701 ttacatttat cgtatgtaca gtacttatct caattttttg ctctttccat aacaactcaa
     2761 tagcagattt aatttccttc ttggttgcat cgtttgctac acgaaacact gcttgattat
     2821 ttttttctcc cagaaaggta gctttctcag atacatacgg ggctaaaata atctttagca
     2881 atcgctcatg ctttagattt agatttacat tgctcatacg agaagctcct caattttttt
     2941 taacccttcc gtagtaatca atatcttctc aaacttcaga agattaaccg ggtctacatt
     3001 tttaac
//
LOCUS       DAORVT010000141         2991 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_29537_length_2991_cov_3.692439, whole genome shotgun sequence.
ACCESSION   DAORVT010000141 DAORVT010000000
VERSION     DAORVT010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29537_length_2991_cov_3.692439"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..2603
                     /gene="gyrB"
                     /locus_tag="PK389_03625"
     CDS             189..2603
                     /gene="gyrB"
                     /locus_tag="PK389_03625"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007160138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HQY22827.1"
                     /translation="MSENTYDSTNIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEV
                     VDNSIDEALAGYCTSILVTIHMDESVTVKDNGRGIPVDIHEEEGRSAAEVIMTILHAG
                     GKFDANSYKVSGGLHGVGVSVVNALSEVLNLRIRRDGKVYEQVYRDGEPDAPLAEVGE
                     TDATGTEIHFKPAGTTFTSIKFNYDILAKRLRELSFLNLGVKIHLVDERTNKEDVFSY
                     EGGIRAFVELLNRNKEVVNPQIFYFSTERQGIVVEVAMQWNDTYQENLFCYTNNIPQR
                     DGGTHLAGFRGALTRTLNTYLEQEGYLKKAKVATTGDDAREGLTVVLSVKVPDPKFSS
                     QTKDKLVSSEVRPAVEAAMGEKFQEYLMENPPAAKAIALKIIDAARAREAARKAREMT
                     RRKGALDIAGLPGKLADCQEKDPALSELYLVEGESAGGSAKQARDRRTQAILPLKGKI
                     LNVEKARFDRMISSVEVGTLITALGCGIGREDYHPEKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFFRQMPEIIEQGHIFIAQPPLYRVKRGKQIQYVKDDKDLNEYLIQIALEDAQL
                     FVNPVAPPIGGATLERLAKQYLHVMDIFARLSHRYPTTALEALIYIKPVTLTQLQNES
                     EMQSWIREFQQQVEQHVKNGTHFQLSLTEDRERKVYLPSIQMMSHGVESFYRFSYELF
                     ISHDYQAIVALGDAVRDLLETGAFIQRGEKKVSILSFKDALAWLMEEAKKGQQISRYK
                     GLGEMNPDQLWETTMDPSVRRMLQVKIDDAVAADQIFTTLMGDNVESRREFIETNALL
                     VGNLDI"
     gene            2641..>2991
                     /locus_tag="PK389_03630"
     CDS             2641..>2991
                     /locus_tag="PK389_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22828.1"
                     /translation="MFSQMFSQLGIISDVRVFFAAPLIADLKSDNYKYDGKSLNLRLI
                     EMNDQQLEEILALFLEKKLSSKIGSLNVQGNNLQFLPASIGEFTELWSLDISNNRLET
                     LPETLWNCKKLKCLN"
BASE COUNT          926 a          530 c          685 g          850 t
ORIGIN      
        1 ccttggcaga ggcagggcgg tgttgagtgg tgagcttgca cataaattta agttaagtaa
       61 ctcttgtcgc agaagttata gcgggagcac ctgaacaatc gatgtttttc tggctattat
      121 tctctaaaaa tgagacaatt aacgctgttt aacaagtatt ttaaggcaac ctaatgagtg
      181 aacccataat gtctgaaaac acctatgatt cgaccaatat taaagtccta aaaggccttg
      241 atgcggttcg aaagcgtcca ggtatgtaca tcggtgatac agatgacggt acgggtctac
      301 atcacatggt attcgaagtg gttgataact ctattgacga ggcgcttgcc ggttattgca
      361 catctatttt ggtcactatc catatggacg agtccgtcac ggttaaagac aatggtcgtg
      421 gtatcccagt tgatattcat gaagaagaag ggcgttctgc cgcagaagtc attatgacta
      481 tcttacatgc aggcggaaaa ttcgatgcca attcctacaa agtctctggt ggtttgcatg
      541 gcgttggagt atctgtcgta aatgccctgt ccgaagtatt aaatctccgt attcggcggg
      601 atgggaaagt ttatgagcag gtttatcgtg atggagagcc agacgcgcca ttggcagagg
      661 ttggcgaaac tgatgcaaca ggtacggaaa ttcactttaa accagcagga acaacgttta
      721 cgagcattaa atttaattat gatattctgg ccaaacgatt aagagaatta tcttttttaa
      781 acttgggcgt taaaattcat ctcgttgatg aacgaaccaa taaagaagac gttttttcat
      841 acgaaggcgg tattcgcgcc tttgtagaac ttttaaatcg caacaaagaa gttgttaacc
      901 ctcaaatctt ttatttttca acagagcgac agggtattgt cgtagaagtt gctatgcagt
      961 ggaacgatac gtatcaagaa aatctatttt gttataccaa taatattcct caacgcgacg
     1021 gtggaacaca tttggctggg tttcgggggg ctttaacccg aacgttaaat acctacttag
     1081 aacaagaagg ttatttaaag aaagcaaaag tcgccacaac aggtgacgat gctcgcgaag
     1141 gtttgacggt tgttttatcg gtaaaagtgc ccgatccgaa gttttcttcg caaactaagg
     1201 ataaattggt ttcttctgaa gtgaggcctg cagtagaagc ggcaatgggt gagaaattcc
     1261 aagaatattt aatggaaaac ccacccgcgg caaaagcgat agcgcttaaa attatcgatg
     1321 cagcgcgtgc gcgtgaagca gcgcgaaaag caagagagat gacgcgcaga aaaggtgctt
     1381 tggatattgc aggcttgcct ggaaaattag cagattgcca agaaaaagat cccgcattgt
     1441 ctgagttgta tctggtagag ggcgagtctg ctggtggatc agctaaacaa gcgcgtgatc
     1501 gtcgaacgca agctattttg ccgttaaaag gtaaaatcct caatgttgaa aaagccagat
     1561 ttgacagaat gatatcttca gtggaagtgg gtactttaat tacagcctta ggttgtggaa
     1621 ttgggcgaga agattaccat cctgaaaaat taagatatca cagcattatt attatgaccg
     1681 atgctgacgt cgatggttcg catattcgga ctctattgtt aaccttcttt tttagacaaa
     1741 tgcctgaaat tatagagcag ggccatatct ttattgctca gccgccattg tatcgtgtta
     1801 aacgcggtaa acagatacaa tatgttaaag acgataaaga tttgaatgaa tatcttattc
     1861 aaattgcatt agaagatgcg caattatttg taaatccagt agccccccct atcggaggtg
     1921 ctacattgga gcggcttgca aaacaatatt tgcatgttat ggatattttt gcacgtttgt
     1981 ctcatcgtta tcctacaacc gcgttagagg ctttaattta cattaaaccc gtcacgctga
     2041 cccagttaca gaatgaatcc gaaatgcaat cctggataag ggaatttcag caacaagtag
     2101 aacaacacgt caaaaatgga acgcattttc aattaagcct aacagaagat cgcgaacgaa
     2161 aggtttattt gccgagtatt caaatgatgt cgcatggcgt agagagcttt tatcgtttct
     2221 cctatgagct tttcatttcg catgattatc aagcgatcgt tgcattaggg gacgctgtgc
     2281 gcgatcttct agaaactggg gcttttatac aaagaggtga gaaaaaagtt tcaattttaa
     2341 gttttaaaga cgccctagcc tggttaatgg aagaagctaa aaaaggccaa caaatatcac
     2401 gttataaagg tttgggagag atgaatcctg atcagctatg ggaaacaacg atggatccca
     2461 gtgtgcgtcg catgttgcaa gtaaaaattg atgatgccgt tgccgcagat caaattttta
     2521 ccacgttaat gggcgataat gttgaaagtc gtagagaatt tattgagacc aatgcattat
     2581 tagtagggaa tttagatatt taattcagcg atgctgagtt tttttattaa agggagtaat
     2641 atgtttagtc aaatgtttag tcaattagga ataatatccg atgtaagggt attttttgcg
     2701 gcacctctga ttgctgattt aaaaagcgat aattataaat acgatggaaa atcgctcaac
     2761 ctcaggctta ttgaaatgaa cgatcaacag cttgaagaaa tattagcctt atttcttgag
     2821 aaaaagctat cttctaaaat tggtagcctt aatgttcaag gcaacaatct gcaattcctg
     2881 cctgcgagta ttggggagtt taccgagcta tggagccttg atatatctaa taaccgattg
     2941 gaaacattac cagaaacgct ttggaactgt aaaaagttga aatgcctaaa t
//
LOCUS       DAORVT010000142         2975 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_29839_length_2975_cov_4.309589, whole genome shotgun sequence.
ACCESSION   DAORVT010000142 DAORVT010000000
VERSION     DAORVT010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29839_length_2975_cov_4.309589"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PK389_03635"
     CDS             <1..365
                     /locus_tag="PK389_03635"
                     /inference="COORDINATES: protein motif:HMM:NF026190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22829.1"
                     /translation="DASLSGSVERTSRIFSSLKGEGIDPILVLWAITREVRAIVAIFC
                     KIKNGQISSTFLSEQGVWRHREPLIKTFINRFSLASLEKILLQSKKTDDIVKGRLQGS
                     AWDSLFSICLTLTGAYHE"
     gene            358..705
                     /gene="rsfS"
                     /locus_tag="PK389_03640"
     CDS             358..705
                     /gene="rsfS"
                     /locus_tag="PK389_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010957657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HQY22830.1"
                     /translation="MNNLKTIILETLEDMKAIEIITLDVRTLTSVTDYMIIATGNSNR
                     HATSIANRIVQRVKENGVLPIGVEGENEGEWILVDLGDVVVHIMLARAREFYSLEKLW
                     SRFPVTTYATENI"
     gene            709..1179
                     /gene="rlmH"
                     /locus_tag="PK389_03645"
     CDS             709..1179
                     /gene="rlmH"
                     /locus_tag="PK389_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HQY22831.1"
                     /translation="MRLQIIAIGTKMPQWTEMAFSEYAKRFSREFPLTLTEIPMPKRS
                     RANTIDRYITEEGTTMLAAIPQKSITIALDERGKLWNTEELARQINYFQNNTPQLNFL
                     IGGPDGLSKACFEKAQIQWSLSPLTLPHPFVRILLVEQLYRAISILNNHPYHRA"
     gene            complement(1190..>2975)
                     /locus_tag="PK389_03650"
     CDS             complement(1190..>2975)
                     /locus_tag="PK389_03650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQY22832.1"
                     /translation="MSTGKPITIAEKCYAPDGTICVYFSSKVPLRDKFGNVIGLLGNS
                     IDITAQKQNEQFLKEAKERAETANLAKSEFLSVISHEFRIPLTGILGMAQLIQMQNLT
                     PEKQKEYVQHISTAGAHLLNLINDTLDFAKLEANKFELALAPMDLNALIEEIFTMLRP
                     LAKAKALELLMHFEPAVPHLIWGDKRVLRQIIINLVGNAIKFTEMGRVSLQVECIEQT
                     ADFATLVVSVVDTGIGIPEEKQGMIFDHFSQVDASHSRRYGGAGLGLTITKQLVELMS
                     GTISVTSQITKGSTFRCVIKFALQKEASAQTPWMAYESTVRILIVDDTPRGNVIQKQL
                     SLSNSQVVSSKEAFSTLLASYQLSDPYDIVIIDQRLEDIDPCELARLILQHEELEQPM
                     LVVLVDNGSVNTKNLAKAAGFFECLTKPIQPFALQVALTAAWESWVEKREMRSLPVFM
                     PLSDGETNPMTAFNNNDSTYKLKVLLVEDDEIVQIVHNNYLQALNCFVDIANNGQEAL
                     EKLNGTYDLVFLDMGLPDIHGMDVIKEFRKRTRGKKQIPVVSLTGYGAESSKQEFMSA
                     GVDDVMVKPVFLEQLEKIIQKYCKEKLQ"
BASE COUNT          896 a          626 c          547 g          906 t
ORIGIN      
        1 ttgatgcaag cctttcgggc tcggttgaaa gaacttctcg catcttttcg agtctaaaag
       61 gtgaggggat agatcccata ttggttttgt gggcaatcac aagagaggtg agagcgatcg
      121 ttgcgatatt ctgtaaaata aagaatggac aaatttcaag cacttttttg agtgagcaag
      181 gtgtttggag gcatcgcgag cctttaatta agactttcat caatcgattt tctctcgctt
      241 cacttgaaaa aattctactt caatcaaaaa aaacagatga tattgtcaag ggtcgactgc
      301 agggctcagc atgggactcg ctgttttcta tttgtctaac gctaacagga gcctatcatg
      361 aataatctaa aaacgattat attagaaaca ttagaggaca tgaaggcgat tgaaattatc
      421 acgctagatg ttcgaacctt aacgagtgta acagattata tgattatcgc gacaggcaac
      481 tctaaccgtc acgccacctc tatcgcaaat cgcatcgttc aacgggtcaa agaaaatggg
      541 gtattgccca tcggcgtaga aggtgaaaac gaaggtgaat ggatcttggt agatctcggt
      601 gacgtagttg tgcacatcat gctcgctcga gcacgtgaat tttatagtct tgaaaaactc
      661 tggtctcgat tccctgttac cacttacgca acggaaaata tttaagcggt gcgtctacag
      721 attattgcta ttggaaccaa aatgccacaa tggaccgaaa tggcattttc agaatatgcc
      781 aagcgctttt cacgcgaatt tcctttaacg ctcacagaaa ttccgatgcc taaacgatct
      841 cgtgcaaata caattgatcg ttatatcaca gaagaaggca ctacaatgct agcggccatt
      901 cctcaaaaat cgataacgat tgccttagat gaacgcggta aactttggaa tacggaagaa
      961 ctcgcaagac aaataaatta ttttcaaaat aacacgccac aacttaattt tttgattggc
     1021 ggacccgatg gattatctaa agcctgtttt gaaaaagcgc agatccagtg gtcgttatcg
     1081 cccttaactc taccgcatcc attcgtacgc atattattgg tagagcaatt atatcgagcc
     1141 attagtatcc tcaacaacca tccctatcat agagcttaac attcctcact cactgtaatt
     1201 tttctttgca atatttttgt atgatttttt ctaattgttc taaaaagacg ggcttaacca
     1261 taacgtcatc aacgcccgcg ctcataaatt cctgtttgct agattcagca ccataacctg
     1321 ttaaagagac aacaggtatt tgcttttttc cgcgcgttcg ttttctaaac tcttttatga
     1381 catccatgcc atgaatgtcg ggcaaaccca tatctaaaaa aactaaatca tatgtaccgt
     1441 ttaatttttc taaagcttct tgaccattat tggctatatc aacgaagcaa ttcaacgctt
     1501 gtaaataatt attatgaaca atttgaacaa tttcatcgtc ttcaaccagt agcactttaa
     1561 gtttatatgt actgtcgtta ttattgaagg ctgtcatagg atttgtttct ccatctgaaa
     1621 ggggcataaa aacaggtaat gatctcattt ctcttttttc aacccagctt tcccaagcag
     1681 cggtaagagc cacttgtaac gcaaagggtt gtatgggttt ggttagacac tcaaaaaaac
     1741 cagccgcttt tgctaaattt tttgtattga ctgagccgtt atcaactaaa acaaccaaca
     1801 tgggttgctc taactcttca tgttgtagaa ttaatcgcgc taattcacaa ggatcaatat
     1861 cctcaagtcg ttgatcaata atcacaatgt cataaggatc agataattga tatgaagcta
     1921 atagcgtact aaaggcttct ttgctcgata cgacttggct attcgaaaga cttaattgct
     1981 tttgaatcac atttccacgt ggtgtatcat caacaattaa tatacgtact gtagattcat
     2041 atgccatcca aggtgtttga gcgctcgctt ctttttgtag cgcgaattta atcacacaac
     2101 gaaaagtgct tcccttggta atttgactgg tgacgctgat ggttccactc ataagctcta
     2161 ctaattgttt agtaatcgtt aacccaagcc ctgcaccgcc atatcgtctt gaatgagaag
     2221 catcgacttg cgaaaaatga tcgaaaatca tcccttgctt ttcttccgga atccctatcc
     2281 cggtgtcaac aactgaaacg acgagtgtag caaaatcagc ggtctgttct atgcattcta
     2341 cttgaagaga gaccctgccc atctccgtaa atttgattgc attacccacc aaattaataa
     2401 taatttgtcg caagacacgt ttgtcacccc aaattaaatg aggaacagca ggttcaaaat
     2461 gcatcagtaa ctctaatgct ttagcctttg caagaggcct taacatggta aaaatttctt
     2521 caattaaagc atttaaatcc attggggcta atgcaagttc aaacttattc gcttctaatt
     2581 tggcaaaatc aagcgtatca ttaattaaat ttaacaaatg tgcacctgcc gtagaaatat
     2641 gttgtacata ttctttttgt ttctccggtg taagattttg catctgtata agctgtgcca
     2701 tacccaaaat acccgtcaat ggaattcgaa actcatggct tatcacggat aaaaattcag
     2761 atttcgcaag gtttgcagtt tccgcacgct ctttcgcctc tttaagaaac tgttcatttt
     2821 gtttttgcgc cgtgatatca attgaatttc caagcaggcc tatgacattg ccaaacttat
     2881 ctcgcaaagg aactttactc gagaagtaaa cgcatattgt gccatccggc gcatagcact
     2941 tttctgcaat agtaatcggt tttcctgtgg acata
//
LOCUS       DAORVT010000143         2969 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_29946_length_2969_cov_2.383665, whole genome shotgun sequence.
ACCESSION   DAORVT010000143 DAORVT010000000
VERSION     DAORVT010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29946_length_2969_cov_2.383665"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PK389_03655"
     CDS             <1..456
                     /locus_tag="PK389_03655"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY22833.1"
                     /translation="EDAEAYFQVNQEPRVIEFLRGPLTMEQVNDFISAVNSHGDKHGY
                     TLWAACLKETGELIGFIGLNYTDWESHFTSAVEVGSRLGSQYWDKSYAIEGAKACLEY
                     GFKKCSLKGIISFTVPSNVCSMRVMEKNGLKRDLNGDFAHPLKNAAVTQ"
     gene            465..974
                     /locus_tag="PK389_03660"
     CDS             465..974
                     /locus_tag="PK389_03660"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY22834.1"
                     /translation="MIIREPAMTDKAAFIQAMQRSQALHSPWVVSPQTEEKFDEYIQR
                     SQKANQKYFLACNAVGNIVGVFNVSEMVLGAFQSAYLGFYSVENYAGQGYMSYGLKLV
                     LKNIFEELKLHRIEANIQPENVRSINLVKANGFSKEGFSPRYLNINGVWCDHERWALT
                     YEDWIKCKL"
     gene            962..1837
                     /locus_tag="PK389_03665"
     CDS             962..1837
                     /locus_tag="PK389_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012461199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HQY22835.1"
                     /translation="MQAIKHNILSIYGEKGKQWLDNLPNLITQLTETYGLSNLKPVNN
                     LSYNYVLTGLQDSKAVILKLGLDIDGFKREAAALMAFSGVGVVEVLIENNGLLLLECA
                     VPGVSLKSYFPENDDEAINITANVIKRLHKAPIPSAHAFPHIKDWLTVLDGDLKIPDQ
                     TLQKARGLRDQLLKTADPDILLHGDLHHDNILKNGEDWVVIDPKGVIGEPTYEVAAFI
                     RNPMPELLTHENVQKIIHNRITRFAKILELSEHRVIDWCFVQAVLSWVWAIEDGCDDT
                     YSKNLTAFFERYTDV"
     gene            1830..2630
                     /locus_tag="PK389_03670"
                     /pseudo
     CDS             1830..2630
                     /locus_tag="PK389_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011976100.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
     gene            complement(2755..2844)
                     /locus_tag="PK389_03675"
     tRNA            complement(2755..2844)
                     /locus_tag="PK389_03675"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2808..2810),aa:Ser,seq:gga)
BASE COUNT          890 a          555 c          673 g          851 t
ORIGIN      
        1 gaagacgcag aggcgtattt tcaagtcaat caagaaccca gagtgatcga gtttttacgt
       61 ggcccattaa ccatggaaca ggtcaacgat tttatatcag cggtaaatag tcatggcgac
      121 aaacatggtt atactttatg ggctgcctgc ttgaaagaaa ccggtgaatt gataggcttt
      181 atcggtctca attataccga ttgggaatcc cattttacct ctgcagttga agttggttcg
      241 cggctagggt cgcaatattg ggacaaaagc tatgcgatag aaggcgccaa agcttgtcta
      301 gagtatggat ttaaaaagtg cagccttaaa ggaatcattt cttttacggt accttctaat
      361 gtttgctcta tgcgagtcat ggaaaaaaac ggattaaaac gtgatttaaa tggcgacttt
      421 gcacaccctt taaagaacgc agctgtaaca caatgatcag aaatatgata atccgcgaac
      481 ctgccatgac agacaaagcg gcctttattc aggccatgca gcgtagccaa gctttacatt
      541 caccttgggt agtttcgcca caaaccgaag aaaaatttga tgaatatatt cagcgttcac
      601 agaaagctaa tcaaaaatat tttttagcat gtaacgctgt aggcaatata gtaggtgtct
      661 tcaatgtcag cgagatggta ctaggggcat ttcagagcgc ctatttagga ttttattctg
      721 tagaaaatta cgcaggccaa gggtacatga gctatggctt aaaattggtt ttaaaaaata
      781 tatttgaaga acttaagctg catcgcatag aggccaacat acaacctgaa aatgttcgat
      841 caataaattt agttaaagct aatggtttct ctaaagaagg cttctctcct cgatatttaa
      901 atatcaatgg ggtgtggtgc gatcatgaac ggtgggcttt aacttatgag gactggatta
      961 aatgcaagct ataaagcata acattttaag catctacggc gaaaaaggaa agcagtggtt
     1021 agataatttg ccgaacctca ttacacaact aacagaaact tatgggctat caaatttaaa
     1081 acctgtaaat aatttaagct acaattatgt gctaactggc ttgcaagatt ctaaagctgt
     1141 tattctaaaa ttaggtctgg atattgatgg attcaaacgt gaagctgcag cactgatggc
     1201 tttttcaggg gttggtgttg tcgaagtgct catcgaaaat aacgggttgc ttttgcttga
     1261 atgcgcagta ccaggcgttt ctttaaaatc ctattttcct gaaaacgatg atgaggctat
     1321 taatatcact gcaaatgtca taaaacgatt gcacaaagca cccataccaa gtgcccacgc
     1381 atttccccac atcaaagatt ggcttacagt attagatggc gacctgaaaa tcccagacca
     1441 aactcttcaa aaagcgagag gacttcgtga tcagctttta aaaaccgctg atccagatat
     1501 cttgcttcat ggcgatttac atcatgataa tattttgaaa aatggcgaag attgggtagt
     1561 gatcgatccc aaaggcgtta ttggtgagcc aacctatgaa gtagcagcct ttatcaggaa
     1621 ccctatgcca gaattattga ctcatgaaaa tgtacaaaag atcattcaca atcgcatcac
     1681 tcgttttgcc aaaattctgg agttatcaga acacagagtc attgactggt gttttgtaca
     1741 agcggtgctg agctgggtgt gggcaatcga ggatggttgt gatgatactt actctaaaaa
     1801 tttaacagca ttctttgaga gatatactga tgtctgatca cgataaaatc cctatagatg
     1861 tgcctctcgt gcaacggctt attgcagagc aatttccaga gtgggctgac cttgagataa
     1921 agccagtcga acttagtgga tgggataata aaacatttca tcttggcgcg tacatgactg
     1981 ttcggttacc aagccatgct gaatactcag gctaagttga aaaagagcag tattggctgc
     2041 ctaaattagc agcgcactta cctttgccaa ttgctatgcc actggcgatg ggcaagcctt
     2101 cagccttcta tcccttgcat tggtctattt ataaatggct tgatggcagc accgcatcga
     2161 ttgaacggat taaagatttg agccaatttg ctaccgcatt aggtgaattt ttaaatgctc
     2221 tacagcaata tgatgccact ggtggcccac ttgcaggaga gcataatttt tatcgtggtg
     2281 gttctctttt aatctatgat actgaaactc gagaagccat agaaaacttg gaggataaaa
     2341 attacgctaa agcgtgtaca caagtatggg acttggcgct tgcctctgct tggaaacaac
     2401 caccagtttg gattcatggt gatattgctg taggtaatct cttagtgaac aacggccaat
     2461 taagcgctgt gattgatttt gggcaacttg gcatcggcga tcctgcatgc gatttggcga
     2521 ttgcatggac tttgtttaga ggggaaagtc gagaggcatt ttgtgcagca ctcaagcttg
     2581 atagcgctac ctgggctcgc ggccgtggtt ggaccttatg gaaagcctta tgttgggcat
     2641 ttcccggtga aaaacgtgtt gactggcgtg ttgttaaggc ttatgaatac tggggttcag
     2701 atagtggctt tggaatttga gaaatgacgg gaaaaaagtt tgagcgctgt aaaatggcgg
     2761 agagggaggg attcgaaccc tcggtacgga ttaacgtaca cacactttcc aggcgtgctc
     2821 cttaagccgc tcggacacct ctccaaattt tagaggctta ggataaccta ttaacagtaa
     2881 gaattaaata agaatcaaaa aagcgtgtta aatagctata gaattttttt catttttgtt
     2941 ctttattctg gcttgttttg aaaaatatt
//
LOCUS       DAORVT010000144         2948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_30294_length_2948_cov_3.059108, whole genome shotgun sequence.
ACCESSION   DAORVT010000144 DAORVT010000000
VERSION     DAORVT010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30294_length_2948_cov_3.059108"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /gene="secD"
                     /locus_tag="PK389_03680"
     CDS             complement(<1..487)
                     /gene="secD"
                     /locus_tag="PK389_03680"
                     /inference="COORDINATES: protein motif:HMM:NF025098.2"
                     /note="part of the preprotein secretory system; when
                     complexed with proteins SecF and YajC, SecDFyajC
                     stimulates the proton motive force-driven protein
                     translocation, and appears to be required for the release
                     of mature proteins from the extracytoplasmic side of the
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HQY22836.1"
                     /translation="MPTPFPRWKTYLLLGILLVGFVYAIPNVYGEDPAVQISGLKGQI
                     INAETTAAVTQLLKDASLPPKSIGIEKEGALLVRFSNSESQLKAKDVIKKALNENYSV
                     ALNLAPVTPHWLSAIGAKPMKLGLDLRGGVHFLMEVDVASVIARRMGGYFSEMPKVLR
                     EA"
     gene            complement(534..806)
                     /gene="yajC"
                     /locus_tag="PK389_03685"
     CDS             complement(534..806)
                     /gene="yajC"
                     /locus_tag="PK389_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007111584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HQY22837.1"
                     /translation="MTQGIMLAAFAVIFYFLFIRPQSKRAKEHQQLVTGLQKGDEVVT
                     TGGLLGKITKLSDNFFVMMIAEGVEVPVQRNAIAMSVPKGTIKSLG"
     gene            1034..1120
                     /locus_tag="PK389_03690"
     tRNA            1034..1120
                     /locus_tag="PK389_03690"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1068..1070,aa:Leu,seq:caa)
     gene            complement(1146..1494)
                     /gene="rnpB"
                     /locus_tag="PK389_03695"
     ncRNA           complement(1146..1494)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK389_03695"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1651..2910
                     /locus_tag="PK389_03700"
     CDS             1651..2910
                     /locus_tag="PK389_03700"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQY22838.1"
                     /translation="MPLTVFALPSKNLEPALAKLNADIQKFVQSKQIPGCAVAVVYRN
                     NVVFMNTYGVRMVGKPEKIDVDTLFQLGSVSKPIAATLASVLEHKGLLKLDDPVTHYL
                     PNFSLKSNQSPHTLRIRHILSHSTGVPRAGFNNLIKAHAPYDRILETLQATRVRTPMG
                     KRYDYHNAMYALISDITRSATHLPFKDALHANLLQPLNMTRTSATLGGLINTLNRATP
                     HTRGNRGALTPSDTYSRGYYAVAPAGGINSSIKDMSIFLKAQMGGYPEVLTHKMLARI
                     QTPQVVTPCTLGPNSGPPNMIKNAHYALGWRVVDFDHHKLVFHGGWVKGFTNFIAFMP
                     DQQLGIVVLHNSENRFSAKTAVKFFESYLNIPHQPCNRLQTKTKLIYQKNRLIRRKTT
                     TFVKCPKKTIANKKLKKKGKAPVKRLV"
BASE COUNT          823 a          708 c          588 g          829 t
ORIGIN      
        1 tggcctcgcg caacactttt ggcatttctg aaaaataacc tcccatgcgt ctagcgatca
       61 ccgaagccac atctacttcc attaaaaaat gtaccccgcc tcttaaatct aaaccaagtt
      121 tcataggctt tgcaccgatt gcgctcaacc aatgcggtgt aacgggtgcc agatttaaag
      181 caacactata attttcgttt aaggcttttt taataacatc tttggctttt agctgtgatt
      241 cggaatttga aaaacgcact aatagtgcac cttctttttc aataccaatg gatttgggtg
      301 gcagtgaagc gtctttcaat aactgcgtaa cagcggctgt tgtttctgca ttaatgattt
      361 gccctttcaa ccccgaaatt tgtacagccg gatcttcgcc atacacatta ggaatggcgt
      421 aaacaaaacc aacgagtaaa atacccagca ataaataagt cttccatcgt ggaaaaggtg
      481 ttggcacggc aattattcct ctgttttaat actgtagggg cagaccgctg tgtctagcct
      541 agcgatttga tcgtgccttt gggtacagac atcgcaatcg catttctttg aacaggcact
      601 tctacaccct ccgcaatcat catcacaaaa aagttgtctg atagtttcgt aattttgcct
      661 aaaagaccac cggttgttac cacttcgtcg cctttttgca aacctgtcac caattgttgg
      721 tgctctttcg cgcgtttact ttggggtcgg ataaacaaga aataaaaaat aacggcaaaa
      781 gccgctaaca taatgccttg ggtcatagcg ccacctgaac cggcattggc agctgttgat
      841 gcggcggctg cctcagcgaa tgcttgtggg gcaactaaaa atagtaagct aaatagcgtt
      901 atgctttgct gtttcatatt tttgatctca cttttccata aaaataagaa catatgctac
      961 tagccaatgc ctaagggcgc aatggggaca ttgacctcgt ggatagatat gttaaacttc
     1021 ggtctcatct ctagccggga tggcggaact ggtagacgcg ccagactcaa aatctggtaa
     1081 tggcaacatt gtgagagttc gattctctct cccggcacca ttgtaaaaaa aaaaattaaa
     1141 aagtaggtag gagtcggctg ataagccggg ttctgtcgtg gacagtcatt cctctaaggc
     1201 catcaatcac tcaatggctc aagcgaccta cccgaaccca gtgcgggccg cactattagg
     1261 ttcctatttg gtcttgctcc aagtggggtt ttccctgccg ttgctgttac caccaacgcg
     1321 gtgcgctctt accgcacctt ttcaccctta ccttaataaa ttaaggcggt acattttctg
     1381 tggcactttc cgtagactcg cgcctcccag gcattacctg gcactttgcc ctatggagcc
     1441 cggactttcc tctctgctcc ctttcggaaa cagagcgact gtctagccaa ctcctgattg
     1501 caagagtaac ggttataggc catcatatgc aaccttattt catgctaaaa tattcctatg
     1561 gctgtaacga tacgattaat cagggctctt cgtaaacaca atgacagatt gtctatagtc
     1621 cgctgtctct tatttttcgt ctcttgtttc gtgccgctta ctgtattcgc acttccctct
     1681 aaaaatcttg aacctgccct cgccaaactt aatgcggata ttcaaaaatt tgtacaaagc
     1741 aaacaaatcc cgggatgtgc tgttgctgtt gtctatcgca acaacgttgt atttatgaac
     1801 acctacggtg ttaggatggt tggtaaaccc gaaaaaatag atgtagatac cctgtttcaa
     1861 ttaggctctg tttctaaacc catagcggca acactggctt ctgtattaga acacaaggga
     1921 cttttaaagc tggatgatcc cgtcacgcat tatttaccga atttttcgct taaaagtaat
     1981 caatcgcctc ataccctaag aattagacat attttaagcc actcgaccgg tgttccacga
     2041 gctgggttta ataatttgat taaagcccac gcgccttacg atcgtatttt agaaacactt
     2101 caagcaacac gcgtaagaac acccatgggt aagcgctatg attaccataa tgcaatgtat
     2161 gctcttatta gcgacattac gcgctctgca acgcatctgc cttttaaaga tgccctgcat
     2221 gcaaatttat tacaaccgct caatatgacc cgaacctccg cgacactggg aggattaatc
     2281 aatacactca atcgtgccac accgcataca cgcggtaatc gcggcgcttt aacaccttca
     2341 gacacttatt ctaggggata ttatgctgtt gcaccggcag gtggtattaa ttcttccatt
     2401 aaagacatgt ctattttttt aaaagcacaa atgggtggct atcctgaagt cctaacgcat
     2461 aaaatgctcg cacgcattca aactccacaa gtcgtaacac cttgcacgct gggtcctaat
     2521 agtgggccac cgaatatgat caaaaatgcg cactacgcgc tcggttggcg tgtggtcgat
     2581 tttgatcatc ataaactcgt gtttcatggc ggttgggtaa agggctttac aaattttatt
     2641 gcatttatgc cagaccaaca acttggtatc gttgtgctcc ataacagcga aaatcgattt
     2701 tctgctaaaa ccgctgtcaa gttttttgaa tcttacttaa atattccgca tcagccatgc
     2761 aatcgtttac aaaccaaaac gaagctgata taccagaaaa ataggctgat tcgacgtaaa
     2821 acaacaacct tcgtaaagtg tcctaaaaaa acaatcgcta ataaaaagct caaaaagaaa
     2881 ggcaaagcac ctgttaaacg cttggtttga gatcaccccc ttagaaaaag ggcgtcacgg
     2941 caggtggg
//
LOCUS       DAORVT010000145         2944 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_30365_length_2944_cov_3.535133, whole genome shotgun sequence.
ACCESSION   DAORVT010000145 DAORVT010000000
VERSION     DAORVT010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30365_length_2944_cov_3.535133"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            354..893
                     /locus_tag="PK389_03705"
     CDS             354..893
                     /locus_tag="PK389_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22839.1"
                     /translation="MQHLKLWLSEHANDRHYLFTLHDLRALCPTLSDPAFKTLLSRAV
                     RSELLLRLCRGIYLYKKSAILDGLLLFHTAALLRAKAFNYISLETVLSDAGVISQIPM
                     NWVSIMSSGRSRIMSCGDFGTIEFIHTNQKPTDLINQLTYDHHCGLWRANVALALRDM
                     KITHRNCDLINWDIAHELI"
     gene            880..1761
                     /locus_tag="PK389_03710"
     CDS             880..1761
                     /locus_tag="PK389_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HQY22840.1"
                     /translation="MNLFDQLVTEALKNQPNLSSLRIVVEKELLHHDILRTLSQNNLL
                     TDLTFIGGTCLRACYGGIRLSEDLDFTGGKGFSRNSLSTMGRILTHSLNEKYGLQVSV
                     SEPVKDIKNVDTWKIKIETRPQQKNLPAQQINIDICAISSYEKQPMVLLNPYGVDMGT
                     NGLVLQTQSRAEIYTDKLIAFALRPNRLKYRDLWDMVWLHQQGIKPNFSLIPSKLQDR
                     KLTQEYFLNLFTERHELLLQDNHLALEFNKEMRRFLSPDQINKTIAQNNLWSFIVYLI
                     GDLDTQLRKSLVNEHKV"
     gene            complement(1764..2540)
                     /locus_tag="PK389_03715"
     CDS             complement(1764..2540)
                     /locus_tag="PK389_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019952696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY22841.1"
                     /translation="MTRFKENIIGLYTILRREISRFLRIWSQTLLPSVISMTLYFVIF
                     GNLIGSRIGEIGGVSYIEYIVPGLIMMAIITNSYGNVVASFFGSKFQRNIEEMLVSPL
                     PNFIILWGYLLGGLARGVAVGILVTGLSLFFTALPLTHIWLVILVAVLTAILFSLAGL
                     INGIFAKKFDDINIVPTFVLTPLTYLGGVFYSIDFLPSFWQWISYSNPIFYMINAFRY
                     GMLGKSDINVHVALWVIIGFIVVLYSWAIWLFRKGVGIKM"
     gene            complement(2537..>2944)
                     /locus_tag="PK389_03720"
     CDS             complement(2537..>2944)
                     /locus_tag="PK389_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY22842.1"
                     /translation="ELRRSMWAFLREINAKGTTIILTTHYLEEAESLCRGIAIINKGE
                     IIENTSIKELLSRLNTETIVVDLKRPLAEAPLLLGCETVSKDETTLEITFPKTLNLND
                     IFITLSKLNIEVLSMRNKANRLEELFIRLVNPV"
BASE COUNT         1040 a          561 c          505 g          838 t
ORIGIN      
        1 agacagcgag gaactggcca atgccctaat cgttttaatc gaaaataaag cgttgcgatt
       61 acaaatggga caagccggca gaaagcgtgt tgcacagtac ttttcagaca cgctcatcta
      121 tgaagaaatc ttaaaattat attcttgaga agggattcgt taggaaaacg tgatcatatg
      181 ccatttttat gttgataaaa gtggcagaca atcacgtttt accaacataa aaatggcata
      241 tgactccggg caagcgttag aggtaagcaa tccatgtctt tcccccttga cacacactat
      301 actaaaggtt acatttagtg taacttaaag tagagtcata tgctatgcgc cccatgcaac
      361 acttaaaact atggttatca gagcatgcta acgacaggca ctatttgttt acgctgcatg
      421 atttacgagc actttgtcca acattatcgg atccggcttt taagacgtta ctaagcagag
      481 cagtacgatc cgaattactt ttgcgtctat gtcgaggaat atatctatat aaaaaatcag
      541 ccattttgga tggcttgctt ttgtttcata cagcagctct gcttagggca aaagctttta
      601 actatatcag tttagaaacg gttctcagtg atgcaggtgt catttcacaa attccaatga
      661 actgggtatc cattatgtca tctggtagaa gccggattat gtcttgtggt gattttggga
      721 ccattgaatt tattcacacg aatcagaaac ctacagatct tatcaatcaa cttacatacg
      781 atcatcattg cggactatgg cgtgccaatg tcgcgcttgc attaagagac atgaaaataa
      841 cgcaccgaaa ttgtgattta atcaattggg atattgcaca tgaacttatt tgatcaatta
      901 gtaacagaag cactaaaaaa ccaaccaaat ttatcatcat taagaattgt cgttgaaaaa
      961 gaattactgc atcacgatat cttaagaaca ttaagccaaa ataatttact cacagactta
     1021 accttcatcg gtggaacatg ccttagagcc tgttatggcg gcattcgatt aagtgaagat
     1081 ttggatttta caggtgggaa gggtttttct cgcaatagtt tatctacaat gggacgtata
     1141 ttaacccata gtctaaatga aaaatatggc ctacaggtct ccgtcagcga accagtaaaa
     1201 gacataaaaa acgtggatac ctggaaaata aaaattgaaa cgaggcctca acaaaaaaat
     1261 ttaccagcgc aacaaattaa catagatatt tgtgcgatct cttcatatga aaaacaacct
     1321 atggtattgc taaatccata tggtgttgat atgggcacaa atggcttggt cttgcaaaca
     1381 caaagtcgcg cggaaattta tactgataag ttaatcgctt ttgcactacg ccccaataga
     1441 ctaaaatatc gtgacttatg ggatatggtg tggttgcatc aacagggaat aaaacctaac
     1501 ttcagcttaa tacctagcaa gctccaagat cgaaaattaa cacaagaata ttttttaaat
     1561 ttgtttactg aacgacatga gttattattg caagataatc acttggcact agaatttaat
     1621 aaagaaatgc gtcgattttt atcacctgat caaattaaca aaacgatagc acaaaacaat
     1681 ttatggtctt ttattgttta tttaataggt gacttagata ctcaattgcg aaaaagtttg
     1741 gtaaacgagc acaaagttta gtcttacatc tttataccaa cgccttttct aaataaccaa
     1801 attgcccaac tatagagcac gacaataaaa ccaataataa cccaaagcgc aacatgaaca
     1861 ttaatatccg acttccctag catgccatat cgaaatgcat taatcatata aaaaatgggg
     1921 ttactataag atatccattg ccaaaaagat ggtaaaaaat caatcgaata aaatacgcca
     1981 cccaaataag taagcggggt taaaacaaaa gttggcacaa tattaatatc atcgaatttt
     2041 ttcgcaaaaa taccattaat taaacctgct aaagaaaaca atattgccgt taacaccgcc
     2101 actaaaataa ctagccaaat atgcgttaag ggtaatgctg taaaaaacaa tgacagaccc
     2161 gtgactaata taccaactgc gacaccgcgt gccaatccgc ccaataaata cccccacaaa
     2221 ataataaaat taggcaaagg cgacaccaac atttcttcga tattgcgctg aaatttagaa
     2281 ccaaaaaaag aagcgacaac atttccatag gaatttgtta taatggccat cataataagg
     2341 cctggaacaa tatattctat atatgaaacc cctcctattt ctccaatgcg agagccaatc
     2401 aaattgccaa atatgacaaa ataaagagtc atgctaatca cagatggcaa tagcgtttga
     2461 gaccaaatac gaagaaagcg ggatatctca cgtcgtaaga tggtatacaa accaatgata
     2521 ttttctttaa atcttgtcat acagggttta ccaagcgaat aaataattct tctaaacgat
     2581 ttgctttatt ccgcatactc agcacttcaa tgttaagctt agaaagcgtg ataaaaatat
     2641 catttaaatt aagcgttttg ggaaaggtaa tttctaatgt tgtttcatct ttagaaacgg
     2701 tttcacaccc taatagaagt ggtgcctctg ctaacggtct ttttaaatca accacaattg
     2761 tttcggtatt taggcgagac agtaattctt taattgaagt gttttctata atttcgcctt
     2821 tgttaataat cgcaatacca cgacataaag actctgcctc ttctaaataa tgggttgtta
     2881 aaataatggt ggttcctttc gcattaattt cacgtaaaaa cgcccacata gaacgtctaa
     2941 gctc
//
LOCUS       DAORVT010000146         2918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_30880_length_2918_cov_2.789032, whole genome shotgun sequence.
ACCESSION   DAORVT010000146 DAORVT010000000
VERSION     DAORVT010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30880_length_2918_cov_2.789032"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..>424
                     /locus_tag="PK389_03725"
     CDS             124..>424
                     /locus_tag="PK389_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HQY22843.1"
                     /translation="MKYRERGFSLIELMIVIAIIGILAAIAVPAYRNYIYKARFSELL
                     VFADNASRQVGEYVIVTGATAIDAATCTAMTNKASVNGTNIMTSWAISNACVVSVV"
     assembly_gap    425..434
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(570..1517)
                     /gene="lipA"
                     /locus_tag="PK389_03730"
     CDS             complement(570..1517)
                     /gene="lipA"
                     /locus_tag="PK389_03730"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005770840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HQY22844.1"
                     /translation="MATTHPTDKQKGQAKMARIPIKIEPSENTLAKPDWIRIRATHAP
                     AVAELKALLREHNLHTVCEEASCPNLNECFAHGTATFMIMGDKCTRRCTFCDVAHGRP
                     DPLNPDEPKNLAKAIAAMNLRYVVITSVDRDDLRDGGAGHFSQCIQEIRERCPNIQIE
                     ILVPDFRGRMEAALDQLSKAPPDIFNHNVETVPRLYKAVRPGSDYTWSLKLIKEFKAR
                     FPLIPTKSGLMLGLGETDEEIQTVLNDMRDYHCDRLTIGQYLQPSKFHSPVARFVTPA
                     VFHALGKYAETLGFTHVASGPLVRSSYHADLQAAGESIK"
     gene            complement(1522..2163)
                     /gene="lipB"
                     /locus_tag="PK389_03735"
     CDS             complement(1522..2163)
                     /gene="lipB"
                     /locus_tag="PK389_03735"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HQY22845.1"
                     /translation="MTNLIIRTLGLMPYDTTWQLMKAFTHQRTLSTPDEFWLVEHFPV
                     FTQGQAGKPEHLLNPGTIPIIQSDRGGQVTYHGPGQLVMYVLINLKRKNLGVKLLITH
                     LEHAIISLLAQHNILGTTQIKAPGVYVNQCKIASLGLRVHRGCSYHGVSLNVSMDLEP
                     FSRINPCGYPNLKVVQMRDFNHAVSTTEVASQLITHLMQQLDYGLIAEKVEGF"
     gene            complement(2173..2433)
                     /locus_tag="PK389_03740"
     CDS             complement(2173..2433)
                     /locus_tag="PK389_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020485010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HQY22846.1"
                     /translation="MQETLINFPCQFPIKIMGLANDTFEGIVVAILHQHVPNLGEGAI
                     VSKLSTGGKFESITATINAQSQEQLDAIYRALTQDPNILMVL"
     gene            complement(2510..>2918)
                     /locus_tag="PK389_03745"
     CDS             complement(2510..>2918)
                     /locus_tag="PK389_03745"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF019555.2"
                     /note="penicillin-binding protein 5; removes C-terminal
                     D-alanyl residues from sugar-peptide cell wall precursors;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HQY22847.1"
                     /translation="NNSARIDETNKLLTYGFRFYESRKLYPALTALKKGRVWHGREKE
                     VDLGLAEDLYVTIGQGQYDNLKTTITVENLIKAPTNKGAALGTLTVQLDNKTISECPV
                     VALTDVEAGGFINRLYDNVTLSVLSLVEKVKPK"
BASE COUNT          802 a          570 c          649 g          887 t
ORIGIN      
        1 gcgcgtgaca tacaacaaat attataagac tttttcagat tttatcaatg caacagtacg
       61 ttaaatcaat aaatagtcta ctctgataga taggaatgat taaaatcaag ggagaaaggt
      121 gcaatgaagt atagggaaag aggattttcg ttaattgaat tgatgattgt tattgcgatt
      181 atcgggattt tggcagcgat tgccgttccc gcttaccgca attatatcta taaggctcga
      241 tttagtgaat tattggtttt cgcagacaat gcatctcgtc aagtcggtga atatgtcatt
      301 gtaactgggg cgacagccat tgatgctgca acttgtacag caatgaccaa caaggcatcc
      361 gtaaatggta caaacattat gacttcttgg gctatctcaa acgcctgcgt tgtaagtgtg
      421 gttgnnnnnn nnnncctgcg caatttggtg gagtagcaac gaacgttacc tttacaccaa
      481 caatgctcgg agatggctcg gtcacgtggg cttgtacgtc tgccggaagt gcgttcgcgc
      541 ccagtagttg cccataaagg tgcatcgttc tatttaatcg attcgcctgc cgcttgcaga
      601 tccgcatggt acgaagaacg taccagcgga ccgctcgcga catgagtaaa tcctaaggtt
      661 tcagcatact tgccaagcgc atgaaagaca gcgggtgtta caaatcgtgc tacaggcgaa
      721 tgaaatttgc tgggctgcaa atattgtccg atcgttaatc gatcacaatg ataatctcgc
      781 atatcattta aaacggtttg gatttcttca tccgtttctc caagccctag cattaacccc
      841 gatttggttg gaatcagagg gaagcgtgct ttaaattctt tgattaattt taaggaccag
      901 gtgtaatcag aacccgggcg aacagcttta tagagtcgtg gtacggtttc tacattgtga
      961 ttaaaaatgt cgggtggtgc tttactcaac tgatctaagg cggcttccat tcgacctcga
     1021 aaatcaggca ctaaaatttc aatctgaata ttgggacagc gctctcgaat ttcttggata
     1081 cattgtgaaa aatgtccagc gccaccatcg cggagatcat ctctgtcaac agatgtaatc
     1141 acaacgtatc gtaaattcat tgcggctatt gctttagcaa gatttttagg ttcgtccgga
     1201 ttgagcggat caggtcgacc gtgtgcaaca tcgcaaaagg tacagcgtcg tgtacattta
     1261 tcgcccataa tcataaatgt cgcggtgcca tgtgcgaaac attcattcag atttggacaa
     1321 gaagcttctt cacaaactgt atgaagatta tgttctcgca ataatgcttt tagttcggca
     1381 acagctggtg catgggttgc tcgaatgcgg atccaatcgg gtttcgctaa tgtattttcg
     1441 ctgggttcaa ttttaatggg aatgcgagcc atttttgctt gacccttttg tttatctgtt
     1501 ggatgtgttg ttgccataat tttaaaatcc ttccactttt tcagcaataa gaccatagtc
     1561 gagttgctgc attaaatgtg ttatcaattg cgaggcaacc tcggtggttg agacagcatg
     1621 attaaaatca cgcatttgca ctacttttag attgggatag ccacagggat tgatccggct
     1681 aaagggttct aaatccatgc tgacatttaa gcttacacca tgatagctac aaccgcgatg
     1741 aactcgaagg ccaagagaag caattttgca ttgattgaca tagacgccgg gtgctttgat
     1801 ttgtgttgtt cctaggatat tatgctgagc gagtaatgaa atgatagcgt gttctaagtg
     1861 agttattaat aatttgacgc ccaaattttt tcttttcaga ttgattaata catacatcac
     1921 cagctgtcca ggtccatgat acgtaacttg tccgccgcga tcactttgaa taataggaat
     1981 tgttcccgga tttaaaagat gttctggttt tcccgcttgt ccttgtgtaa atacaggaaa
     2041 gtgttctacc agccaaaatt cgtctggcgt agaaagcgtg cgttgatggg taaacgcttt
     2101 cattaactgc caggttgtgt cgtatggcat taaacccaat gttcgaataa taagattagt
     2161 catgcatcgt ctttataata ccatgagtat attgggatct tgcgttaatg cacgatagat
     2221 agcatccagt tgttcttggc tttgggcgtt tattgtggcg gtgatagatt cgaatttccc
     2281 acccgtgctg agtttagaaa caatggcacc ttctccgaga tttggcacgt gttgatgcaa
     2341 aatagcaaca acaatacctt caaaggtatc atttgcgagt cccattattt taatgggaaa
     2401 ctgacaagga aagtttatca atgtctcttg cataaaaatc ccacgagcgg agagatacgg
     2461 gcatctttca agggaataaa atgatcgttt aagtaagcaa caggggctgt catttaggtt
     2521 taactttttc tacaagcgat agaacagata acgtgacatt atcgtataat cgattgataa
     2581 atccgcctgc ttcaacatcg gttaaggcaa caacggggca ttcactaatc gttttattat
     2641 ctaattgaac tgtgagtgtg ccaagagcag cgcctttatt ggtgggcgct ttaatgaggt
     2701 tttctactgt aatggtcgtt ttcaaattat catattgacc ctggcctatt gtaacataaa
     2761 gatcttccgc aaggcctaaa tcaacttctt tttcgcgccc atgccatacc cgtccttttt
     2821 taagtgcggt gagcgcaggg taaagttttc gagattcata gaatcgaaaa ccataggtta
     2881 ataatttatt ggtttcatca atacgtgccg aattgttt
//
LOCUS       DAORVT010000147         2907 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_31078_length_2907_cov_3.075386, whole genome shotgun sequence.
ACCESSION   DAORVT010000147 DAORVT010000000
VERSION     DAORVT010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31078_length_2907_cov_3.075386"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            943..2070
                     /locus_tag="PK389_03750"
     CDS             943..2070
                     /locus_tag="PK389_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22848.1"
                     /translation="MAKSIEFKMLLGAELEKSLGKEKSFTEKYPKKSYSTGELKVNLK
                     NWQKSVSKQVEPQIKLTRIAAVPIKEKEVTSLRAIHESATPTAEAIVIQKMTPEKIVG
                     TLFSSINTLEFIKMEAEYKTRLKDAKSPAAKAKVDEDWKNVVQAGSDVFAAGGLKGVN
                     EVKLRSFSTELTKNKNNFTAIVNIANTGLAVSGLTAQAVTAQSALQAGFVPQTGVLLD
                     EGLIVTEIKNLCSSPFASGTFTKHFHKGFSLVVQIPYWCPTWRKPWRVCYKNVTLAGL
                     SLDINVQVGYRVTCCGATVWGQASAQACATLVGITFCAGCSARIIGVAGIGRTGSGSN
                     CTYGIGINAQLTCTFGNVTVLSVQAPFGYNINGPCPPAGLC"
     gene            2439..2717
                     /locus_tag="PK389_03755"
     CDS             2439..2717
                     /locus_tag="PK389_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22849.1"
                     /translation="MEQIQKKKTMSTNSSCCGSYLKRPVSKVKSTTPPNPKVQGGVAV
                     IYLGAGDIQIKGGGTDSLYYASDHNRHFKVYAEDADSVLKHSFIIRKP"
BASE COUNT          881 a          545 c          622 g          859 t
ORIGIN      
        1 tgatagccat catcctgcat atctccaaca ataaaaagat catggggaaa tttacgaatg
       61 gctgggtttc aaacattctg ggatttgcgg ctttgatatt gatgagcgtg gcggcggggg
      121 tgatgatgta tctggagatt gcagaataat ctgctaagtg ataaattctt aatgaaaaat
      181 ttggaaatac agacctaaaa tagtatattc gcttccactt tagaaataaa taattttagt
      241 tgcattacat aataagatgt tttgattgag gtagtgtagg aatttaatga atttaatacc
      301 cgtttagctt aaagcgttag taataatttt agaagggaag ctatcaacaa acaccatttt
      361 ttgactccaa aatatttttc tatgaaacca aacccctctt aatttattta cgttgtagct
      421 ggtctcttgc atgtcagact tgagctatag ccattccctt ttatcatatg attgactgga
      481 gattcagtca accaaaggat tttgggtcat aacctgaagt aagtgatgaa ctgtcattgc
      541 ttacctcatg aggtctatat tacattgtga tatccatttt tggctgcgac gcttgtgtcg
      601 catggtgata tctatgtata ggtgtataag tattgtggat gatatggcga ttctccattg
      661 tattgtcaaa tccgtcgcat attctgaata gtatttaaaa aaaacatttt ataggtacag
      721 accggattta ccatccaacg tcgagcatag cagaacatcg acaccaaata tgaaagtatg
      781 catcagggtt gccgaaaact gaaaagagta ggtataacta aagtatcatc tatgggatat
      841 ccgaagcctt tatcagatgt tcatgtggac atcacaggaa atgtaagcat ttccaggaaa
      901 tattaatcac tttattgtaa ccaattttta aatattagta tcatggctaa gtctattgaa
      961 tttaaaatgt tattaggggc tgaacttgaa aaatcattgg gcaaggaaaa atcgtttaca
     1021 gaaaaatacc ccaagaagtc ctactccaca ggagagctta aggtaaatct gaaaaactgg
     1081 cagaagagtg tatccaagca ggttgaacca caaataaagc tcaccagaat tgccgcagtt
     1141 cctatcaagg agaaggaagt gacaagcttg cgcgccattc acgaaagcgc cactcccacc
     1201 gcagaagcaa ttgtgattca aaaaatgact ccggaaaaaa tagtgggtac ccttttttcc
     1261 tcgatcaata cgctcgagtt tatcaaaatg gaggctgaat ataagacccg attgaaagac
     1321 gcaaaatcgc ctgctgctaa agcaaaggtg gatgaagatt ggaaaaatgt ggtacaggct
     1381 ggttcggatg tgtttgccgc gggaggtttg aaaggagtga atgaagtaaa gctccgtagt
     1441 ttttctactg aacttacaaa gaataagaac aacttcacag cgattgtcaa tattgccaat
     1501 acaggcctgg ccgtaagtgg tttgactgcc caagctgtta ccgcccaatc agccttacaa
     1561 gcgggtttcg tgcctcaaac gggggtgttg ctggatgaag gacttattgt taccgagatt
     1621 aaaaatcttt gttcaagtcc attcgcctct ggcacattca ccaagcattt tcataaagga
     1681 ttcagtctgg ttgtgcagat tccttattgg tgtcccacat ggagaaaacc atggagagtg
     1741 tgctataaaa atgtcacgct tgcaggcctt tctttggata tcaatgtaca ggtagggtat
     1801 cgcgtgactt gctgtggggc cactgtatgg ggtcaggcga gtgctcaggc ttgtgctacg
     1861 ctcgtgggca tcacattctg cgccggatgt tcggcacgaa tcattggcgt agcaggtatt
     1921 ggtcgaactg gttctggaag taattgtacg tacggcatcg gcatcaatgc gcagttgacc
     1981 tgcacctttg gtaatgttac ggtacttagt gttcaagcgc cctttggcta caatatcaac
     2041 ggaccttgtc caccggcagg attatgttaa tgaataattg ttgaacaagt gatcagggcg
     2101 gatagatttc atttgccctg attttcttat aaacatcatc taaacaatgt tactaaaagt
     2161 gtatcctgaa caatggcttt ggctgatcat atgcatcctg tgcgtctggc gtcttacaac
     2221 attgatatgt tatgaagccg gaccatttaa cctgatttct aacatgaggg cagtatacta
     2281 ccgcctcagg ttgggtaagc tcatagattg ctttcattgt gcatctgtat ggatttcaat
     2341 catcataacc ctttgtgtat atgtagtaaa tagttctaca ttctttctgg tcctcgctat
     2401 tgcgggaggc gcatcaatca tcgaaaaatc cttaaatcat ggagcaaata cagaagaaga
     2461 aaacgatgtc cacgaatagt tcgtgttgcg gatcttattt aaaacgacct gtgtcaaaag
     2521 taaagagcac cacacctcct aatcccaaag ttcaaggtgg cgtggcagtt atttatctgg
     2581 gtgcagggga tatccaaatt aaaggaggtg ggactgactc cctctattat gcatcagatc
     2641 ataaccggca tttcaaagtg tacgctgaag atgcagattc ggtcttgaag cattcattta
     2701 tcatacgtaa accataattc cagttgattc aaatttcctt ttctcgattt gaaaggggtt
     2761 taaaaattac ttgaagtatt taatcattta tatcattcct cattttctaa tacgtaccta
     2821 tcatgaaaaa agaaaaaaac cattccttac ttcccgaatt ttttaaaatt caaaaagcat
     2881 cccttgccac ggaagatggc caaaggt
//
LOCUS       DAORVT010000148         2906 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_31102_length_2906_cov_3.034374, whole genome shotgun sequence.
ACCESSION   DAORVT010000148 DAORVT010000000
VERSION     DAORVT010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31102_length_2906_cov_3.034374"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="PK389_03760"
     CDS             complement(<1..624)
                     /locus_tag="PK389_03760"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HQY22850.1"
                     /translation="MAMIDKRSFLLLLVGSLAFFQVEAFQSFVVNSIRVEGLQRVSKG
                     AVLEDLPVQIGQTITDAKATEAIRALYKSGFFKEVTLNRDGNALVVSVIERPTISHLT
                     LTGIKDKDKVKKLLREVGVAEGQLFDPAILLRAQKELERYYYSRGRYGVKIEPTVTEE
                     GSLVSVTFAIYEGDVARIKEIKIMGNTLFPEKELLKEFRTVKTNWLSW"
     gene            complement(686..2053)
                     /gene="rseP"
                     /locus_tag="PK389_03765"
     CDS             complement(686..2053)
                     /gene="rseP"
                     /locus_tag="PK389_03765"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HQY22851.1"
                     /translation="MVQLLISILAFIVAISILVGVHEFGHFWVARRFGIKVLRFSIGF
                     GRPLYRWYDKLGTEYVISAIPFGGYVSLFGERGQVVSPQERHQAFCFKSVWARILVLV
                     AGPFANLLFAVVVYWVLFMMGVSAFIPMLGNVPKDSIAGLAGFHQGQEIVSVEGKSTP
                     SWESVSMQVLAHIGEDKTINVEVRDDAEAPLHNKILDLGIVSGGTSESNWLDSLGLVP
                     LDPVPAIVGNVLPDLPAFKAGLQPGDRILSADGGVITSRMDIIHYVQSRPNKKIHLMI
                     QRGSQKLSLVLETVTKASEEDNKKIGFVGVEFPPLKEMPKSFFRIQRYSVVTALVKAV
                     SQTAEYSVLTLELLKKMIVGKVSARHISGPVSIAKYAGESAAIGLNEFLTFLGVISIS
                     LGVLNLLPIPILDGGHIVYCLAEVITGRRPSEIVQNVGMWIGGLILVGFMILALYNDL
                     MHFLR"
     gene            complement(2038..2601)
                     /locus_tag="PK389_03770"
     CDS             complement(2038..2601)
                     /locus_tag="PK389_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017376211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate delta-isomerase"
                     /protein_id="HQY22852.1"
                     /translation="MGQINKNIEQIVLVDAEDQVIGLEEKLKAHQLGLLHRAFSVFIY
                     RKNPEDSNVIEFLLQQRHVDKYHCGGLWTNACCSHPRLNEDIILAGQRRLKEEMSIAL
                     TLKKVGSFQYKACYENGLTEHEFDHVLLGEHDGVQSIVLDPKEAQDFRWVSERRLQRE
                     LDLYSEHYTPWFKPAFHLVLEDLWFNS"
     gene            complement(2601..>2906)
                     /locus_tag="PK389_03775"
     CDS             complement(2601..>2906)
                     /locus_tag="PK389_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22853.1"
                     /translation="RNNGLHMIVMKSRRKGFSYMEGWDSADTVNLIPYCTVLLAAHDL
                     KYLTKGNQIFGMAKSYLEFLELHTDWNRGFLKKDKETVQALFSKLIIIAPFENGILN"
BASE COUNT          920 a          694 c          474 g          818 t
ORIGIN      
        1 ccaggataac caattcgtct tcacggttct aaattctttt aaaagttctt tttcaggaaa
       61 aagggtgttt cccattatct tgatttcttt aatgcgtgca acatcacctt cataaatagc
      121 aaaggtaaca gaaaccaacg aaccttcttc tgtaactgtt ggttcaattt ttacgccata
      181 acgcccacgg ctataataat agcgttcgag ttctttttgg gcacgtaata aaatagcggg
      241 atcaaaaagt tgtccttctg ccacaccgac ttctctcaag agtttcttaa ctttatcttt
      301 atctttaata cccgtaagcg ttaggtgact aatcgtagga cgctctataa cactcaccac
      361 caaggcattg ccatcacgat ttaaagtcac ttctttaaaa aagcctgatt tgtaaagcgc
      421 acgaatcgct tccgtggctt ttgcgtctgt tatcgtctgc cctatctgta caggaagatc
      481 ttctaagacc gcgcctttag agacccgttg tagtccttca acacgaatgc tgtttactac
      541 aaaagactga aaggcttcta cctgaaaaaa tgcaaggctt ccaactaaaa gtagcagaaa
      601 tgagcgctta tctatcattg ccatcgtttt ggtttcgctg gttaaaaatc ttaaggggca
      661 tcctaccaca aaaataggat ggggttcacc ttaaaaaatg cataagatca ttatataagg
      721 ctaatatcat aaacccaact aatatcagcc cccctatcca catgcccaca ttctgtacta
      781 tttcggaagg tcgacggccg gtaataacct cagcgagaca atacacaata tgtccaccat
      841 ctaaaatggg aatcggcaat agattcaaaa cacccaaact aatgctaata acacctaaaa
      901 aagttaaaaa ttcgtttaat ccaatcgcgg cagactcccc agcatattta gcaatgctga
      961 cgggtccact gatatgacgt gctgacactt tgccaacaat catttttttt aaaagctcta
     1021 aagttaaaac tgaatattcc gctgtttgag aaactgcctt caccaaagcc gttactacac
     1081 tataacgctg aatgcgaaag aatgatttgg gcatttcttt aagcggtgga aattctaccc
     1141 ccacaaatcc aattttttta ttatcttcct ctgaagcttt agtcactgtt tctaatacca
     1201 atgataattt ttggctgcca cgttggatca tcaaatggat ttttttgtta ggccgtgact
     1261 gtacataatg gataatatcc atacgcgatg ttataacccc cccatctgca ctgagaatac
     1321 gatcgcctgg ctgcaacccg gccttaaaag caggtaaatc cggtaaaaca ttacccacaa
     1381 tcgcgggcac aggatcaagt ggcaccagcc ccaaactgtc tagccaattc gattctgaag
     1441 tgccacccga tacaatccct aagtccaaga ttttattgtg caatggcgct tctgcatcat
     1501 cccgcacctc tacattgatc gttttatctt ccccaatatg cgccaatacc tgcatagaaa
     1561 cggactccca actgggggtc gattttcctt cgactgaaac gatctcttgt ccctgatgga
     1621 atcccgccaa gcctgcaata gagtctttgg gcacattccc caacatagga ataaaggctg
     1681 aaacccccat cataaacaat acccaataaa cgacaaccgc aaacaacaaa ttagcaaagg
     1741 gtcccgcgac taataccaat attcgtgccc agacagactt aaaacaaaat gcttggtgac
     1801 gttcctgcgg agaaacgact tgaccgcgtt ctccaaagag cgatacatat ccaccaaaag
     1861 gaatagccga tatcacatat tccgttccaa gtttgtcgta ccagcgatag aggggacgtc
     1921 caaatccaat tgaaaaacgt agcactttaa tgccaaatcg tcttgcaacc caaaaatgtc
     1981 caaactcgtg tacaccgacc aaaatactaa tggcgacaat aaaagccaga atcgaaatta
     2041 agagttgaac cataaatcct ccaacaccaa atgaaatgca ggtttaaacc agggagtata
     2101 atgttctgaa tacaaatcca attctctctg taatcgtcgt tcagataccc aacgaaaatc
     2161 ctgagcttct ttcggatcta atacaataga ttgtacgcca tcatgctctc cgagcaacac
     2221 atgatcgaat tcatgttcag tcaatccatt ttcataacaa gccttatatt gaaaagaacc
     2281 gacttttttt aaagtaagcg caatcgacat ctcttctttt aaacgtcttt gacccgccaa
     2341 tatgatgtct tcgtttaagc gcgggtgact acaacaggcg ttagtccaaa gacccccgca
     2401 gtgatattta tccacatgac gttgttgtaa taaaaattca ataacatttg aatcttcagg
     2461 attttttcta taaataaaca ccgaaaaagc acgatgcaac aaacccagtt gatgtgcttt
     2521 taatttctcc tcaagcccaa tgacttgatc ctcggcatct accaaaacaa tttgctctat
     2581 atttttgttg atttgaccca tcagtttagt atcccatttt caaatggcgc aataatgatt
     2641 agttttgaaa atagcgcctg gaccgtctct ttatctttct ttaaaaaccc tctgttccag
     2701 tctgtatgta attctaaaaa ttcaagataa cttttagcca taccaaaaat ctgattacct
     2761 ttagtaagat atttcaaatc gtgagcagct aataaaacag tacaataagg aattaaatta
     2821 acagtatcag cactatccca tccttccata taagaaaacc ctttacgtct ggatttcatt
     2881 acaatcatgt gaagtccatt atttct
//
LOCUS       DAORVT010000149         2872 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_31750_length_2872_cov_2.706780, whole genome shotgun sequence.
ACCESSION   DAORVT010000149 DAORVT010000000
VERSION     DAORVT010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31750_length_2872_cov_2.706780"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PK389_03780"
     CDS             complement(<1..418)
                     /locus_tag="PK389_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22854.1"
                     /translation="MPLSNANAYTSASDLPQPQHPTDYERNWSDNLATDIKHDFGNYF
                     TSNNLSFFAGTFLTAGVLANTGLDRAFADHYQTDFKTKGTDTFFAFPKGVGGLSFYFI
                     PIFLTSVAVGHLREHTLLGNVLYHWGYRSFRTIVVGG"
     gene            complement(557..1960)
                     /locus_tag="PK389_03785"
     CDS             complement(557..1960)
                     /locus_tag="PK389_03785"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004725536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HQY22855.1"
                     /translation="MKNAKKPIETRLEKDSMGEIKVPKDALYGAQTQRAIDNFPISGI
                     TFPPSFIKTLCLIKSAAAKVNADLKLLKPEIAKAIMDAAEGIIEEQYDTEFPLDIFQT
                     GSGTSSNMNANEVIARLASLKTNLPVHPNDHVNMSQSSNDVIPTAIHVSCSLDIKHML
                     LPALQHLHKIILERAKSLQNTVKTGRTHLMDAMPLTFEQELSGWATQIQHNITRIAET
                     LIRLQALAIGGTAVGTGINAHPEFGKRVSIELYKKTQIPFTPHTNYFMALSSQDTIVE
                     LSGHLRVIAISLSKMANDLRLMNSGPLAGFNDIVLPSLQPGSSIMPGKINPVIPEAVL
                     MACAQVMGNDTTISIAGLSGNFQLNTMLPVIAYNILLSIKLMSNSALALADKAIAGFV
                     INQEHIEQQLSRNPILVTALNAIIGYEKGAEIAKRAYREKRSIKAVAAEMTNLSSAEL
                     DKLLDPKILTKGGIEKQ"
     gene            complement(1957..>2872)
                     /gene="acnA"
                     /locus_tag="PK389_03790"
     CDS             complement(1957..>2872)
                     /gene="acnA"
                     /locus_tag="PK389_03790"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747496.1"
                     /note="catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HQY22856.1"
                     /translation="SKETQMLIQETVQANMFVKRYQAVFTGDERWQNLKIPKGEQYDW
                     NNTSTYVKLPRYFEGMKQKPPKMLANISNARVLAILGDSVTTDHISPAGNIAKNSPAG
                     EYLQQLGIAPADFNSYGSRRGNHEVMMRGTFANIRLKNQLVRGTEGNWTLHLPSGEKM
                     SIFDAAMRYQQEHCPLILMAGKEYGSGSSRDWAAKGPRLLGVQAVIAESFERIHRSNL
                     IGMGVLPLQFKPNENVTTLNLSGREIYNIDLENLRHRGTITIHLTTETGEKRTFSAIV
                     RIDTPKEMEYYRHGGILHYVLRQLLEKL"
BASE COUNT          767 a          568 c          632 g          905 t
ORIGIN      
        1 gtccaccgac aacaatggtt ctaaagcttc ggtatcccca atgataaagg acattcccga
       61 gtaaagtgtg ttctcgtaaa tggcctaccg caacgctcgt tagaaagatc ggtatgaagt
      121 aaaacgagag tccacccact cctttaggaa atgcaaaaaa agtatccgtt cctttggttt
      181 taaaatcagt ttgataatga tcagcaaatg cgcgatctaa gccggtattg gctaaaacac
      241 ctgcggttaa gaaggtacca gcaaaaaacg ataaattatt agaagtgaaa taattcccga
      301 aatcatgctt gatatccgtt gccaaattat cagaccaatt tctttcgtaa tcggttggat
      361 gttggggttg tggtagatcg gatgcgctcg tgtatgcatt tgcattggac agaggcataa
      421 tccctatcag acaacatgtg attaataatc gaaatgattt ttttaaattt ctcatatccc
      481 tataccggtt ggtgtatcct acttatcttt agcatggatc ataggtggga taacgtcaaa
      541 gttactatgt aactaactat tgtttttcga tgccgccttt tgtaaggatt ttgggatcga
      601 gaagcttatc taattcggct gacgaaagat tggtcatttc agcagcgacg gctttgatag
      661 atcgtttttc gcgataagcg cgtttagcga tctctgcacc tttttcataa ccaataatcg
      721 cgttcagtgc ggtgacgaga ataggattgc gtgatagttg ttgttcaata tgttcttgat
      781 tgataacgaa tccagcgatc gctttatcgg ctaatgcgag agcgctatta ctcatcaatt
      841 ttatgctgag taaaatatta taagcaatca cgggtaacat ggtatttaat tgaaagttgc
      901 cagaaagtcc tgcgatacta atggtggtat cattgcccat gacttgagcg cacgccatta
      961 acacggcttc tggtataact ggattaattt tccccggcat aatgctgcta ccgggttgaa
     1021 ggcttggcaa aacaatgtcg ttaaagcccg caagtggtcc actgttcatg agacgtaaat
     1081 catttgccat tttagataaa ctaatcgcga taacacgcaa atgtccagaa agttctacaa
     1141 tagtatcttg cgaacttaaa gccataaaat aattggtgtg tggcgtaaaa ggaatttgtg
     1201 tttttttgta gagttcaatg ctaacccgtt ttccaaattc tgggtgtgca ttaatgccag
     1261 tgccaacagc cgtgccgcca atggccaaag cctgaagtcg tattagcgtt tcggctattc
     1321 tggttatgtt atgctgaatt tgtgttgccc agccgcttaa ttcttgctca aaggtcagag
     1381 gcatggcatc cattaagtgt gtgcgtcctg ttttaactgt gttttgtagg ctttttgccc
     1441 gttctaagat aatcttatga agatgctgta atgcgggcag tagcatatgc tttatatcta
     1501 aactgcaact tacatggata gcggttggaa tgacatcgtt actgctttgg ctcatgttga
     1561 cgtgatcatt cggatgaacg gggagattgg tttttaagct cgctaatcgc gcgatcactt
     1621 cgttggcatt catgttgctg ctggtacccg atccggtttg aaaaatatcg agtggaaatt
     1681 cggtgtcata ttgttcttca ataataccct cagcagcatc cataatggct tttgcaatct
     1741 cgggtttcaa taatttaaga tcggcattta cttttgcagc cgcacttttt atgagacaga
     1801 gtgttttaat aaaagaagga ggaaaggtta ttccactgat cggaaaatta tcaatagcac
     1861 gttgtgtttg ggcaccatag agtgcatctt taggcacttt gatctcgccc atgctgtctt
     1921 tttcaaggcg tgtttcgata ggttttttcg cgtttttcat agtttctcca acaattgtcg
     1981 caatacataa tgtaaaatgc cgccatgccg ataatattcc atctctttgg gtgtatcgat
     2041 tcgaactatg gcagaaaacg ttcttttttc tcccgtttcg gttgttaaat gaattgtaat
     2101 tgtgccgcga tggcgtaaat tttcaagatc aatgttgtag atttcacgtc cgctgaggtt
     2161 aagcgtcgtg acattttcgt tcggcttaaa ttgtaagggc agtacaccca tgccgattaa
     2221 attggaacgg tgaatacgct caaagctttc tgcaataacg gcttgaacac cgagtaaacg
     2281 agggcctttt gccgcccaat cgcgtgaaga accgctacca tattctttac cggccattaa
     2341 aatcagggga caatgttctt gttgatagcg catagctgca tcaaagatag acattttttc
     2401 gcccgagggt agatgcagtg tccaattgcc ttctgtgcca cggacaagct gattcttgag
     2461 acgaatatta gcaaaagtgc cgcgcatcat cacttcgtga ttcccacgtc gtgatccata
     2521 agaattaaaa tcggcaggtg cgatacctaa ctgttgtaaa tattcacctg cggggctatt
     2581 ttttgcaata ttgccagcag gtgaaatgtg atcagttgta acagaatcgc ctaagattgc
     2641 taatacccgc gcgttactaa tattagcgag cattttgggc ggtttttgtt tcatcccttc
     2701 aaaataccgc ggcagtttaa cgtaggtgga agtattgttc caatcatatt gttcgccttt
     2761 gggaattttt aaattctgcc agcgctcatc tcctgtaaag acagcttgat aacgttttac
     2821 aaacatattg gcttgtaccg tttcttggat cagcatttgc gtttctttgg aa
//
LOCUS       DAORVT010000150         2818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_32859_length_2818_cov_3.152733, whole genome shotgun sequence.
ACCESSION   DAORVT010000150 DAORVT010000000
VERSION     DAORVT010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32859_length_2818_cov_3.152733"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..969
                     /locus_tag="PK389_03795"
     CDS             13..969
                     /locus_tag="PK389_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HQY22857.1"
                     /translation="MGSIWLSYQLTRLTGKPIQWGTPMTISIEPTTACNLRCPECPSG
                     LRSFTRETGNMKEDFFRKVIDEMHRKLIYLIFYFQGEPYINPKFLEMVRYAHQKGIYT
                     ITSTNGHFLHPENARKTVESGLDRILISIDGSTQEVYEQYRKEGHLATVLEGARNLVA
                     AKEEAGSSTPHIVFQMLVVKPNEHQIGEVQALAKEIGVDEVKLKTAQVYDFVEGNELI
                     PTISKYSRYYQDHAGVWHVKNKLRNQCWKLWHACVITWDGMVVPCCFDKDATYRLGDL
                     REKSFSSIWKGTSYRQFRARLLQGRDKIDICTNCTEGCKVWA"
     gene            1791..>2818
                     /locus_tag="PK389_03800"
     CDS             1791..>2818
                     /locus_tag="PK389_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22858.1"
                     /translation="MFSVPGFDEVIKHPQFGDLQPIDEVVHKIVNTSSNPWLANGLWW
                     YKCFFIIDFVWAACLLSYLYRRVRESTDWLSENPLKVKGLPRLLFIAVIAAYFSDVCE
                     NFMYLRCLDSQNCNYFSGIVDFKVFAYAVVFLLVVIFLYQTHVYDHLNKIRRSLRATF
                     LSIIAILLLAGLATLMEQGSTVIIHLLENRWSLSGAIILINLLALASAHYPDYLDKYF
                     SAEIKCASATPCPNSPIVKWEISGLFGKKPFWGIGVITYKDLTSKTPFVNTRKETNRQ
                     LGDVESKFFGHFRKIIGLLILVTWMYVLLYVIAKYKYLTLPLQSTTILILTCVLLFYF
                     HCARVREE"
BASE COUNT          770 a          578 c          607 g          863 t
ORIGIN      
        1 aaattactca atctgggctc catctggtta tcgtaccagt tgacccgact gaccggcaaa
       61 ccgatccaat ggggtacccc tatgaccatt tccattgaac ccactacagc gtgcaatctc
      121 aggtgtcctg aatgcccgag tggactcagg tcctttaccc gggagaccgg aaatatgaaa
      181 gaggattttt tcagaaaggt gattgatgaa atgcaccgga agctaatcta cctcatcttt
      241 tattttcaag gcgaaccgta tatcaatcct aaattcctcg agatggttcg atacgctcat
      301 cagaaaggta tctatacgat cacttccacc aacggtcatt ttcttcaccc tgaaaatgcc
      361 cgtaagactg tcgaatcagg gttggatcgc attcttattt ccatcgatgg ttcaacacaa
      421 gaggtgtatg agcagtatcg taaggaaggc catctggcta ccgtccttga aggggcgcgt
      481 aatctggtag cagccaaaga agaggcaggt tcatcaacac ctcatatcgt tttccagatg
      541 ctggtcgtca aaccaaacga gcatcagatc ggggaggtgc aagctcttgc aaaagagatc
      601 ggtgtagacg aagtgaaact gaaaactgcg caggtttatg atttcgttga gggtaacgaa
      661 ctcattccta ccatttcaaa atacagtagg tattatcagg accatgccgg cgtgtggcat
      721 gtcaaaaaca aattacgcaa tcaatgctgg aagctctggc atgcctgcgt catcacctgg
      781 gatggaatgg tggttccgtg ttgttttgat aaggatgcta catacagact tggtgatctg
      841 agggaaaaat cattttcctc catctggaaa ggcacatcct atcggcaatt ccgcgccaga
      901 ttattacagg gcagggataa aattgacatt tgcaccaact gtaccgaagg ttgtaaggtt
      961 tgggcataac cgggatttcc aaataggttc ccttgcacgt tagggcgctt ctaataacta
     1021 tcaaaagggg agcaataact attataatgg gttgacaatc aaaatggata attaattatc
     1081 attgggttag gagaggcgtc ctttagctgc tattctcctg gcaaactagt tttgtatgcc
     1141 cctgatttat ttaaaaataa acatggcaat taattttcac ctagatattc actgaatgat
     1201 tttttattgt acattagacc tatcattaat ttacttccag gttgaataac catattttta
     1261 aattcactag tcagttataa ttgcttctgc gtctgtagtg ttctaggttg ctttcaattt
     1321 attgttttat ttttttcata catccgattt taacgattcg ggggatagcc gcattgactt
     1381 cttccatcgt gtaattgtaa aaattaatgt gttgggcatg gcatgctcat cacggctatt
     1441 catattgttt cattaaaatc atttcacacc gttaaacctt ttaacatgcc gaccactccc
     1501 attccatata ttgttgatcc tagtggccaa ccgcccacca ggccgtacag ccatagtttc
     1561 aaagacgcgt atacattatg tgagggcctt gtgtgtgatc tggtggttga atttgtatcc
     1621 ggcctcaggc gcaagcaggt tctgagaggg cgaacccaga tttcatcggc ggaggttgtt
     1681 gaaagaattt tgtccttgaa tacctcagca ttcatgacaa aatggtttgt atgaaaattc
     1741 atgcgtgggc aacagtgctc ttgacaataa tactagctat tgtcagctgg atgttttctg
     1801 tcccgggatt cgacgaagtc attaagcatc cacaatttgg tgacttgcaa cctatagatg
     1861 aagttgtcca taaaattgtt aatacatcgt caaatccctg gttggcgaat ggattgtggt
     1921 ggtacaagtg tttctttatc attgattttg tctgggctgc ctgtttgctt tcatacctct
     1981 acaggagagt aagggaaagc accgactggc tgagcgaaaa cccgctgaaa gtaaagggcc
     2041 tgccacgatt gctctttatc gcggtgattg ctgcatactt ttctgatgtt tgtgaaaact
     2101 tcatgtacct gagatgcctt gatagtcaaa actgcaatta cttttcaggg attgttgatt
     2161 ttaaagtttt tgcttatgca gttgtctttt tgctggtggt gatcttttta taccaaacac
     2221 atgtctatga tcatctaaat aaaatcagga gatccctacg cgcaactttc ttatctatca
     2281 ttgccatcct cctgttagcc ggattagcta cacttatgga acaggggagt actgtaataa
     2341 tacatttgct tgaaaatcga tggagcctat caggagctat tattctgatc aatttgctgg
     2401 ccctggcaag tgctcattat cccgactacc ttgataaata tttttccgca gaaataaaat
     2461 gtgcttcagc tacaccctgt ccaaacagcc cgattgtaaa atgggagatc agtggtttgt
     2521 ttggcaaaaa acccttctgg gggatagggg ttattaccta caaggattta acttcgaaaa
     2581 ctcccttcgt aaatactaga aaagagacaa accgccaatt gggggacgtg gagtcaaagt
     2641 ttttcggtca tttccgcaag atcatcggtc ttcttattct ggtgacatgg atgtatgtat
     2701 tgctttatgt aattgcgaaa tacaaatatt tgacattgcc attacagagt acaaccattt
     2761 taattcttac atgcgttcta ctcttttatt ttcattgcgc cagggtcagg gaagaatg
//
LOCUS       DAORVT010000151         2793 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_33416_length_2793_cov_2.552958, whole genome shotgun sequence.
ACCESSION   DAORVT010000151 DAORVT010000000
VERSION     DAORVT010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33416_length_2793_cov_2.552958"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PK389_03805"
     CDS             complement(<1..268)
                     /locus_tag="PK389_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQY22859.1"
                     /translation="MERSLLSYLVSWINSEDRKPLIVRGARQVGKTWLVRHLASTCNK
                     TLLEINFEKKLSLVSLFESNDPKHILLNLSAHFNQQIEPGNCLLF"
     gene            complement(452..2233)
                     /gene="aspS"
                     /locus_tag="PK389_03810"
     CDS             complement(452..2233)
                     /gene="aspS"
                     /locus_tag="PK389_03810"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HQY22860.1"
                     /translation="MRTHYCGELNDASIGKTVSVCGWVHYRRDLGGLIFVEIRDRTGL
                     VQAVFSPQEQASALFQQAEQLRKEYVVCITGEVRLRPEGTENKNSPAGKIELGVLALE
                     IIGKAEPLPFYPDEHQNISEEVRLKYRYLDLRRPEMFYRLKFRTDAIRIMRSFFDNHG
                     FLDVETPILTKATPEGARDYLVPSRTQPGSFFALPQSPQLFKQLLMMSGIDRYYQIAR
                     CFRDEDLRADRQPEFTQLDIETSFMDEEAIMQLVETLIRKLFLELLEIALPDPFPRLT
                     YREAIDRFGIDRPDLRNPLELVEISDLVQDVDFKVFSSPAKDKEGRVAALCVPGGVEN
                     LSRKEIDDCTHFVGLYGAKGLAYIKVNQREQGREGLQSPILKFLPDDVLEAILERTRA
                     KTGDVIFFGADQSKIVNDSLAALRNKLGQDLGLLQGEWRPLWVVDFPMFERDKMGTRW
                     QAVHHPFTAPSVSDIGALRADPGHSTSRAYDIVLNGIELGGGSIRIHTPEMQQAVFDV
                     LGVSKTEAQEKFGFLLDALKYGCPPHGGLAIGIDRLIMLMTGATSIRDVIAFPKTQTA
                     SCPLTSAPSSVSEEQLKELGIRIKTAS"
     gene            complement(2274..2609)
                     /locus_tag="PK389_03815"
     CDS             complement(2274..2609)
                     /locus_tag="PK389_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011398682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HQY22861.1"
                     /translation="MPIYEYVCTACGATLEVFQGMKDVPLTDCRVCTKPTLQKLISAT
                     GFRLKGDGWYATGYETDLKKDNDKKKNLIDSTPASTSTPPASSAPNTSESVKATASAS
                     NASKTETSV"
BASE COUNT          792 a          647 c          523 g          831 t
ORIGIN      
        1 aaaacaataa acagtttcca ggttcgattt gttgattaaa atgcgcactc aagtttaata
       61 aaatgtgctt aggatcatta gattcaaaaa gactcaccag cgatagcttt ttttcaaagt
      121 ttatttcaag taaagtttta ttacatgtgc tagcaaggtg tctcaccagc caagttttac
      181 caacttgtct tgccccccgt acgattaaag gctttctatc ttcagaatta atccaggaaa
      241 ctaaatacga aagtaagctt ctttccacga tacatcctca tccgctttaa tcggttatat
      301 tcttggataa taagctattt ttaatcaaaa ttcaaaaaga gacggttgtt tttgaatttt
      361 ttacaaaaag aaccgcctct tttagtgatc tactccagaa aagagacggt tctttttcgg
      421 ggctttagag tcaaatctgc tcccaaagat tctaagaagc cgttttgatc cgtatcccca
      481 attcctttaa ctgctcttct gagaccgatg aaggcgcaga tgttaacgga caactcgcgg
      541 tttgtgtttt aggaaaagca atcacatcgc gaatagatgt tgcacctgtc atgagcatga
      601 ttaaacgatc aatgccaatg gctaagcccc cgtgcggtgg gcaaccatat ttgagtgcgt
      661 ctaataaaaa cccaaatttt tcttgtgctt ctgttttact aacgcccaaa acatcaaaaa
      721 ccgcttgctg catttcgggt gtatggattc gaatcgaccc accgcccaac tctataccgt
      781 ttaaaacgat atcataagcg cgtgatgtag aatgacctgg atctgctctt aatgccccaa
      841 tgtcgctcac actgggtgct gtaaacggat gatgaaccgc ctgccaacgt gtacccattt
      901 tatcgcgctc gaacataggg aaatcaacaa cccatagcgg tcgccattca ccttgcagaa
      961 gccctaaatc ctgacccaat ttattgcgga gtgccgctaa agaatcatta acgattttag
     1021 actgatcggc accaaagaaa ataacatcgc ctgttttagc gcgtgtgcgt tctaaaatag
     1081 cttcgagaac atcatccggc aaaaatttta aaataggcga ctgcaaacct tctcgacctt
     1141 gctctcgttg attaacttta atgtaggcaa gtccctttgc gccataaaga cccacaaaat
     1201 gcgtacagtc atctatttct ttgcgagaga ggttttctac tccgcctggc acacacaaag
     1261 ccgcaacacg cccttcttta tcttttgcag ggctcgaaaa tactttgaag tcgacatcct
     1321 gaaccaaatc agaaatttct actaattcca gaggattacg taaatcgggt ctatcgatcc
     1381 caaaacgatc gattgcctcg cgatacgtca atcgcggaaa gggatcgggt aaagcaatct
     1441 ctaacaattc caaaaataat tttcgaatta atgtttcaac cagttgcatg atcgcttctt
     1501 catccataaa agaagtttct atatccaact gggtaaattc aggttgccta tctgcacgca
     1561 aatcttcatc tctaaaacaa cgtgcaattt gataatagcg atcaatgccg gacatcatca
     1621 acaattgttt aaacaactga ggagactgtg gcagcgcaaa aaaagatccg ggctgtgtac
     1681 gactgggcac taaatagtca cgagcgcctt ctggggttgc ttttgttaga atcggtgttt
     1741 caacatccaa aaagccatga ttatcaaaaa agctacgcat aatacgaatt gcatcggtgc
     1801 gaaactttag tcgataaaac atttctggac gacgcaagtc taaatagcga tatttaagcc
     1861 gcacttcttc tgaaatattt tggtgctcat ccggataaaa tggtaaaggt tcggctttgc
     1921 caataatctc gagcgcaagc acgcccaatt cgatttttcc cgcaggtgaa tttttatttt
     1981 cggtgccttc cggacgtaat cgaacttcac ctgtaataca aaccacatat tcttttcgaa
     2041 gttgttctgc ttgttgaaaa agtgcactgg cttgttcttg tggcgaaaaa acagcctgta
     2101 cgagacctgt tctgtcgcga atttctacaa aaataagccc acccaaatcg cggcgataat
     2161 gcacccatcc gcaaaccgaa actgtttttc caatcgaagc gtcattcaac tcaccacaat
     2221 aatgggtacg catagttttt tcctaaatta taaagtatta atttttgctt agcttatacc
     2281 gatgtttctg tttttgaagc attggaagca gaagccgtag ccttgaccga ttcacttgtg
     2341 tttggagcac tactggcagg tggagtactc gttgacgccg gcgttgaatc gatcagattt
     2401 ttctttttgt cgttgtcttt cttaagatct gtttcatacc ctgttgcata ccagccatcg
     2461 ccttttaatc gaaatccagt tgctgatatc aatttctgca gcgtgggttt agtacacacg
     2521 cgacaatcgg ttaagggaac atccttcatg ccctgaaaaa cctctaatgt cgcaccacat
     2581 gcggtacaaa catattcata aattggcata tgatatcttc ctcattttcg taattcttcc
     2641 cacatcgcac gcattttttc ttcctcgaca tgattcgcgg attgcaccct tctgttttct
     2701 gtgtccggcg ttgcgttttc taattttctc cagccgcctt taggtctccc agcaatgtat
     2761 gtttcttctt caagagcttc gttacgacaa ttg
//
LOCUS       DAORVT010000152         2780 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_33695_length_2780_cov_3.470826, whole genome shotgun sequence.
ACCESSION   DAORVT010000152 DAORVT010000000
VERSION     DAORVT010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33695_length_2780_cov_3.470826"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PK389_03820"
     CDS             <1..308
                     /locus_tag="PK389_03820"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY22862.1"
                     /translation="KWYTKAADQNHAKAQFNLGFLLYEGIGTERNVTTGFSWLQKAAD
                     QDNAKAQYKIGCAYLSGKGVSKDLDLAIKWLSKSAEQGDKDAIHTLGLAREMHAQKH"
     gene            292..1026
                     /gene="ubiG"
                     /locus_tag="PK389_03825"
     CDS             292..1026
                     /gene="ubiG"
                     /locus_tag="PK389_03825"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009669418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HQY22863.1"
                     /translation="MHKNIEVTELDKFDAMAAEWWDPKGKCSPLHALNPTRLQFIVDR
                     YSLLQKNVLDIGCGGGILSESLFKRGAYVTGIDASSAVLKVATLHAMEIKQQENNLHN
                     PSTNTLNYIHTTAEEYATTHPNSFDAVTCLELLEHVPDPLLLIKAAATLVKPGGHLFF
                     STLNRTPKSYLFSVLGAEYLLKLLPRGTHQYEKFIRPAELEQWLRQAGLRLKELAGLQ
                     YNPITHKTYLNDDVSVNYLAHVVKES"
     gene            1062..1784
                     /locus_tag="PK389_03830"
     CDS             1062..1784
                     /locus_tag="PK389_03830"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HQY22864.1"
                     /translation="MTILFDLDGTLVDTAPDFIHALNVVRAEINMLPLKPSNQTSIRM
                     VEVAVTEGLAGLVAAGLKVKAEHPESPLLQKRLLHAYQKNLGLYSTLFPGIEALLKTL
                     EQRHIPWGVVTNKPAQQTHPLMDRLGLSHRAACIVSGDTTPYAKPHPEPLFYACRQIG
                     VSPIECLYIGDAERDIIAGNAAGMTTIAALFGYIEDITLAKNWGAHHYIHHADEILPW
                     FETWERLKNPHEIKSSFEQGIV"
     gene            1781..2647
                     /gene="hpnC"
                     /locus_tag="PK389_03835"
     CDS             1781..2647
                     /gene="hpnC"
                     /locus_tag="PK389_03835"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HQY22865.1"
                     /translation="MSTKRAANTFLENAYQKCEILALTHYENFPVASRFLPKKIRRPI
                     AVIYAFAREADDIADEGDFTIASRLEQLEHYEKNLEKITQGIVPSHPVFIALNDVLKK
                     HPTLPISLFFNLLTAFKQDVIKKEYNDFEEVLNYCKYSANPVGRLLLYLTNNASTENL
                     SYSDDICTALQLINFLQDLDSDLTLRDRCYLPIDEMHAMHISKEDFKTHQQSAAMQNF
                     IITQLLRTERLLQNGSPLGKNLSGLFGFEIRIIINAANTIVQQLKKRRCIYDRPTLKR
                     WHWPKVLLTALK"
BASE COUNT          860 a          611 c          544 g          765 t
ORIGIN      
        1 taaagtggta tacaaaagcc gccgatcaaa atcatgccaa ggcacaattt aacttaggtt
       61 ttttattata tgaaggtatt gggacagagc gtaatgtcac cactggattt agctggctcc
      121 aaaaagccgc agaccaagac aatgccaaag cccaatacaa aatcggttgc gcttacttat
      181 ctggcaaggg agtttcgaaa gatcttgatt tagcgattaa atggctaagt aaatccgccg
      241 aacaaggtga taaagatgcc attcatacat tgggactggc gcgtgaaatg catgcacaaa
      301 aacattgaag taaccgaact cgataaattc gatgccatgg ccgccgagtg gtgggatcca
      361 aaaggcaaat gcagcccctt acatgcatta aatcctacgc gtcttcagtt tatcgttgac
      421 cgctattctc ttttgcaaaa aaatgtttta gatatcgggt gtggtggtgg aatattgtcc
      481 gagagtttat tcaaacgcgg tgcctatgtt acgggtatag atgcaagctc tgctgtttta
      541 aaagtcgcaa cgctacatgc gatggagatt aaacaacagg aaaataattt acataaccca
      601 agcacaaata cattgaatta tattcatacc acagcagaag aatatgccac aacacatcca
      661 aatagctttg atgctgtaac ctgcttggag cttttagaac atgtgcccga tcccttgttg
      721 ctcattaaag cggcagcaac cttggtaaaa cccggcggtc atttattttt ttcaacgtta
      781 aatcgcacac caaaatccta tttgttttcc gttttaggcg cagaatattt gctaaaactt
      841 ttaccacgtg gtacacatca atacgaaaaa tttattcgac ccgcagagct agagcagtgg
      901 ctaagacaag cagggcttcg cttgaaagaa ctcgccggat tgcagtataa tcccatcaca
      961 cataaaacgt atttaaacga tgatgtgtct gttaattatc ttgcacatgt tgtcaaggaa
     1021 tcgtaaggcc agaaggtaca cgaaactaat aggatgatac catgacgatt ttatttgatc
     1081 tcgatggcac acttgtcgat acagcaccgg attttataca cgcactaaat gtcgtacggg
     1141 ctgaaataaa tatgctgcct ttgaaacctt caaatcaaac ttccattcgt atggttgaag
     1201 tcgcagttac agaggggctt gcaggtttgg ttgcagcagg acttaaggtc aaagccgaac
     1261 atccagaaag tcctttattg caaaagcgtc tgctacacgc ttatcaaaaa aatttagggc
     1321 tgtattcaac gttgtttccg ggaattgaag cgctattgaa aacgctagaa caacgacaca
     1381 ttccttgggg agtggtgacc aataaacccg cccagcaaac ccacccactt atggatagat
     1441 tagggctatc gcatcgcgcg gcttgtattg tcagtggcga taccacacca tacgctaaac
     1501 cacatccaga gcctttgttt tatgcctgcc gccaaatcgg tgtttcgcca atagagtgcc
     1561 tctatatcgg cgatgccgaa cgcgatatca ttgccggaaa tgcagccggg atgacgacta
     1621 tcgccgcgtt atttggctat atcgaagaca ttacactcgc aaaaaactgg ggtgcacatc
     1681 attacattca tcatgcagac gaaatcttgc cctggtttga aacatgggaa cgcttaaaaa
     1741 atccacatga aataaagtcc tcttttgagc aaggtattgt ttgagcacaa aacgcgctgc
     1801 taacacattc ttagaaaatg cttaccaaaa atgcgaaata cttgctctca cgcactatga
     1861 aaattttccg gttgcatccc gctttttacc aaaaaaaatt cgtcgcccta ttgccgttat
     1921 ttatgctttt gcaagagaag cggacgacat tgccgatgaa ggtgatttca caatagcgtc
     1981 tcgtctagag caactcgagc attatgaaaa aaatctagaa aaaataaccc aaggcatagt
     2041 gccttctcat cctgttttta ttgcactcaa tgatgttctc aaaaaacatc ctacacttcc
     2101 catctcgctt ttttttaatc tcctcacggc ttttaaacaa gatgttataa aaaaagaata
     2161 taacgatttt gaagaagtct taaactactg taaatattct gcaaatcctg ttggcagatt
     2221 gctgctctat ctcacaaaca atgcatcaac agaaaactta agctattctg acgatatctg
     2281 taccgcacta cagctcatta attttctgca agatttagat tctgatttaa cacttcgtga
     2341 tcgctgttat ttgccaatcg acgaaatgca tgcaatgcat atttccaaag aagatttcaa
     2401 aacacaccaa cagtctgctg caatgcaaaa ctttataata acccagctcc tccgaacaga
     2461 gcgcttatta caaaacggct cgcctcttgg aaaaaactta agcggtttat ttggatttga
     2521 gatccggatc attatcaatg cagccaatac tatcgttcaa cagttaaaaa agagacggtg
     2581 tatatacgac cgccccacgc tcaagcgttg gcattggcct aaggtcttgc tcaccgcttt
     2641 aaaataaggc agaatgcgag actgacgtgc ttttcatgct taacttcact ttttaaaagg
     2701 ggcaattgca atgttattcg gccgatttta cgcatttggt tatcgattta gtcgctttac
     2761 ctctattaaa acgggtattc
//
LOCUS       DAORVT010000153         2742 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_34548_length_2742_cov_3.794939, whole genome shotgun sequence.
ACCESSION   DAORVT010000153 DAORVT010000000
VERSION     DAORVT010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34548_length_2742_cov_3.794939"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="PK389_03840"
     CDS             <1..367
                     /locus_tag="PK389_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22866.1"
                     /translation="DANEENILRRFKDNESSDADKERMRSFTEMRRQEVVAANSKLAK
                     LRGDDGKTPTKSRKSQPEGKKAPAKKRPSIISFLYNRKKEEFAALKNRLKGSSSSDNT
                     PPAPPSPGRPGSRGSQGGG"
     gene            complement(379..1512)
                     /gene="ftsW"
                     /locus_tag="PK389_03845"
     CDS             complement(379..1512)
                     /gene="ftsW"
                     /locus_tag="PK389_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HQY22867.1"
                     /translation="MGAVANRQTAYDPWLLLSLVSLLGFGLVMVTSASLSFAEGSGLP
                     AGYFAIRHAIYLAIGLVGFAVTTTVPLALWRKLSIPLLIITMALLTLLLIPGISRQVN
                     GSSRWLFLGPVSIQVSEFAKLFVVIYMAQYLVQKHKEVQTELSGFIKPMLTLGLVSAL
                     LLLEPDFGATVVIVSTTMGMLFLGGLPIRRFLVLLLPALGALGILSITSPYRMQRLTS
                     FLNPWTDQFNTGYQLTQSLIAFGRGGWFGTGLGNSVQKLLYLPEAHNDFIFAVLAEEL
                     GLVGAMVILLFFGLLVWRILALGRQAILSGHPFAGFMAYGIGLWFGFQAIINIGVNLG
                     VLPTKGLTLPFMSYGGCSLVVGLLAVGILLRIEFELSRKIQRV"
     gene            complement(1519..>2742)
                     /locus_tag="PK389_03850"
     CDS             complement(1519..>2742)
                     /locus_tag="PK389_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQY22868.1"
                     /translation="KEYQQMEEIWKTEKAAIEGTKRLKEALDKAKLDLSVAHRSGDFA
                     KMSEIQYGRIPELEKQLKASSELEVETKLFRNKVTEEEIAEVVARWTGIPVSKMLEGE
                     REKLVKMEEQLKSRVVGQIDALKAVANAVRRSRAGLSDPNRPVGSFLFVGPTGVGKTE
                     VCRALAEFLFDSEHAMVRVDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEIVRRKPY
                     SVILLDEIEKAHTDVFNILLQVLEDGRLTDGQGRTVDFRNTVIVMTSNLGSQMIQELT
                     GQVEYDEIKVAVMGVVSEHFRPEFINRIDEIVVFSPLGKAQIRSIADIQLEHLKARLK
                     AQNLDLEATTTALDKLAEIGYDPIYGARPLKRAIRHVLENPLAEALLTGQFMPGDTIQ
                     VSVQNDKITFLKKGR"
BASE COUNT          881 a          671 c          491 g          699 t
ORIGIN      
        1 tgacgcaaac gaagaaaaca ttctacgtcg gtttaaggat aatgagagta gcgatgcaga
       61 taaggaaaga atgcgtagtt ttacagaaat gcgaagacaa gaggtggtag ctgcgaattc
      121 aaaattagca aaattaagag gtgatgacgg aaaaacgcca acaaaaagta gaaaatcaca
      181 acccgaagga aaaaaagcgc ccgcgaaaaa acggccatcc ataatatctt ttttatataa
      241 ccgaaaaaaa gaagaatttg ctgctttaaa aaatcgttta aaaggctcat cctcttcaga
      301 taacacacca cccgcacctc cgtcacctgg acgccctgga agtcgtggaa gccaaggagg
      361 cggttaagat aaaataaatc aaaccctttg aattttacgc gacaattcaa attcaatgcg
      421 gagcaaaatg ccaacggcta ataatcccac aaccagacta caaccaccat aactcataaa
      481 aggcaaagtt agacctttag ttggcaaaac cccgagatta acgccaatat taatgattgc
      541 ttgaaaacca aaccacaacc caatgccata cgccataaaa cctgcaaacg gatgtccaga
      601 caatatcgcc tgcctaccta aggccaatat gcgccaaact aacagaccaa aaaataacaa
      661 tataaccata gcgcccacca aaccaagctc ttccgccaat acggcaaaaa taaaatcatt
      721 atgggcttcg ggtaaataca gcaacttttg tacactgtta cctaatccgg taccaaacca
      781 accccctcta ccaaatgcaa ttaacgattg tgttaattgg taaccggtat taaattgatc
      841 tgtccaaggg ttcaaaaaag acgttaagcg ctgcatacga tacggtgaag taatcgataa
      901 aatacccaag gcccccagcg cgggtaaaag caataccaag aaacgtcgaa tgggtagacc
      961 acccaaaaat aacataccca ttgttgttga aacgataaca accgtcgcac caaaatcagg
     1021 ttccaataac agtaatgccg aaaccaaacc caaggtcaac atcggcttta taaagcctga
     1081 aagttctgtt tgcacctctt tatgtttttg aaccaaatac tgtgccatgt agataacgac
     1141 aaacaattta gcgaattcag aaacctgtat cgaaacaggc cccaaaaata accatcgcga
     1201 gctgccattg acctgacgac taatacctgg gatcaataat aatgtcagta gcgccatggt
     1261 gatgattaaa agtggaatgc tgagttttcg ccatagagca agcggtacag tggttgtaac
     1321 agcaaaacca acgagcccta ttgctaaata aatcgcatgg cgaatcgcaa aatacccagc
     1381 cggcaaaccg ctcccttcag caaaggaaag cgaggcagag gtgaccatta caagtccaaa
     1441 gccaagcaaa ctcaccaacg agagtaataa ccagggatca taagccgttt ggcgattcgc
     1501 tactgccccc actatttttt atctcccttt tttcagaaag gtaattttat cgttttgcac
     1561 actcacctgt atggtgtctc ctggcataaa ctgccccgtt aataacgcct ctgctaatgg
     1621 attttccaaa acgtgccgaa tcgcacgttt caagggtctt gcaccataaa tgggatcata
     1681 cccaatttct gccaatttat ctaaggctgt cgttgttgct tccaagtcca aattttgtgc
     1741 tttcaatctc gctttaaggt gttctaactg aatatcagcg atgctgcgga tttgcgcttt
     1801 gccaagcggt gaaaatacta cgatttcatc tatccgatta ataaattcag gtctaaagtg
     1861 ttcactcaca acccccataa ccgctacttt aatttcatcg tactcaactt gacccgttaa
     1921 ttcttggatc atttgcgacc ctaaattgga tgtcataaca ataaccgtat ttctaaaatc
     1981 aacggttcga ccctgtccat cggttaaacg accatcttct aacacttgca acaaaatatt
     2041 aaacacatcc gtatgggctt tttcaatttc gtctaataat ataacgctgt aaggcttacg
     2101 tcgcacaatt tctgttaagt atccaccttc ttcatacccc acataacctg ggggcgcacc
     2161 gattaaacgt gcaaccgagt gtttttccat aaattcggac atatcgaccc gaaccatagc
     2221 atgttcgcta tcaaacaaaa attctgcgag tgcacgacag acttccgttt tacccacgcc
     2281 cgttggtcca acgaataaaa aagatccgac cggacgattg ggatcagaca accccgcacg
     2341 cgagcgacga acggcatttg ccactgcttt tagcgcatcg atttgaccca ctacgcgaga
     2401 ttttaattgc tcttccattt taactaattt ctcgcgctca ccctctaaca ttttagagac
     2461 aggaatgcct gtccaacgtg caacgacttc ggcaatttcc tcttctgtca ctttgtttct
     2521 aaacaatttt gtttcgactt ccagttcaga ggaagctttt aattgttttt caagttcggg
     2581 aatacgaccg tattgaattt cagacatttt tgcaaaatcg ccagaacgat gtgcaacact
     2641 gagatcaagt tttgctttat ccagcgcctc tttcaatcgt tttgtgcctt caatggccgc
     2701 tttttccgtt ttccaaatct cttccatttg ttggtattct tt
//
LOCUS       DAORVT010000154         2706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_35393_length_2706_cov_3.465862, whole genome shotgun sequence.
ACCESSION   DAORVT010000154 DAORVT010000000
VERSION     DAORVT010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35393_length_2706_cov_3.465862"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2225
                     /locus_tag="PK389_03855"
     CDS             <1..2225
                     /locus_tag="PK389_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22869.1"
                     /translation="NSVIYQMLDSTLRQTLQIPEEKVFLENIQDMSDAEAAVYQACGA
                     LIPDIANLLALPTLVKTQLQATTGNLHVCSDTGKPLNLKEKTIQAVMRKVRPVANTNV
                     IATLRMIQAGRALRTAVQGSEVPPASPSPTVLETVLGYANNAAELPLSALGSAANREL
                     TKGLRIYLVEESVPEKSIPTPAIIFSRKITPRLGGLITSELIEIKSFKIFKKIRGLAT
                     ALTSLVTNRARTIPMETLENAELKNQSETIVKQNASKTNKISSVLLPLDHPLARLITE
                     QLKHAPEIFDYTKQHKMQQNTLDVQRKRAIDNEALRQQAIELAKIKETAIKCGFGEAF
                     FADFETTPVIQERYTRLLKAINDVDTLPLPIDLLPDEAIKVRQYIKTRLINESEILLS
                     HIDAATERFNALLLNDYSIKEKLAKTENAISTVDNIGWFGTTWLVFTTVAPEAKTHYL
                     ALLELQRSEAKATIAALSKPLEDLKEGQYHLVIDYLKENSENAALLSTEVSHYKKTFW
                     LWQSWPEGLIKLDKYLSESVPNVRIHLPQEETVTPALLFAAAQKPNEPNEPKEMYDYK
                     VEAELQLANALKLSEAEAIAASQSKIGPKTIAGWELTDVPEIGNCFYEAVALQLQKNN
                     FPIAKTFPGTRPEDGGTALVNILRRRSTLEFRDRDWAGEEEILALAKELKIVIAIVDT
                     RSENPEFQYYFANWNGTMGTTRNKTEIPQGQMVAELAYTGNHYLSVRSKPSSPTSPRQ
                     "
     gene            complement(2252..>2706)
                     /locus_tag="PK389_03860"
     CDS             complement(2252..>2706)
                     /locus_tag="PK389_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22870.1"
                     /translation="IPYAVGGCFELEELSLDTIPFLTTVPDTIEGLQLRWQGLLTSLA
                     WFQNERVEMLASIFKILETQAPSDVLEKASEPRLSTKPLQIILGYLTDRTEYKELIPK
                     SPDPVVWGNRVPESLRHLFSIYKQRMIPPQNEGHPQPKKSGISCKNAV"
BASE COUNT          906 a          588 c          535 g          677 t
ORIGIN      
        1 tgaattctgt tatttatcaa atgctagata gcacattaag acaaacgcta caaattccag
       61 aagaaaaagt ttttctcgag aacatacagg acatgagcga tgcagaggcg gctgtttatc
      121 aggcttgtgg tgccttaatt ccagatatag ctaatctttt agcactgcca accttggtaa
      181 aaacgcaatt acaagccact acgggaaatc ttcatgtttg cagtgacaca ggaaaaccgc
      241 taaaccttaa ggaaaaaaca attcaggctg tcatgcgaaa agtacgccct gttgcaaata
      301 caaatgttat cgcgactctt cgcatgattc aagcaggacg agctttaaga acagccgtcc
      361 aaggatcaga ggtcccacca gcatcgcctt cgccaaccgt attggagact gttctaggct
      421 acgccaacaa cgccgcggaa ttgccactct cggcattagg cagcgccgct aatcgtgaat
      481 taaccaaagg gcttagaatt tatttggttg aagaaagtgt gcctgaaaaa tctataccca
      541 cgccggctat cattttctca agaaaaatta ctcctcgctt gggtggtctg ataaccagtg
      601 aattaataga aataaaaagc tttaaaattt tcaaaaaaat acgtggtctt gctactgcgc
      661 tgacatcgct cgtaaccaat agagcacgta ctatccccat ggaaacttta gaaaatgcag
      721 agctcaaaaa tcaaagcgag acaatcgtaa aacaaaacgc gtccaaaaca aataaaatat
      781 cttcagtttt attgccattg gatcatccct tggctcgctt gataacagaa caactaaagc
      841 acgctcccga aatatttgat tataccaaac agcataaaat gcaacaaaac acgctggacg
      901 tacaaaggaa acgcgcaatt gataatgaag cattgagaca acaagcaata gaattagcaa
      961 aaattaaaga gaccgccata aaatgcggct ttggcgaagc gttttttgca gattttgaga
     1021 caacccctgt tatacaagaa cgttatacac gattgctaaa ggcaatcaat gacgtagata
     1081 ctttgccctt gcctattgat ctattacctg atgaggctat aaaggtcaga caatatatca
     1141 aaacacgact cattaacgaa agtgaaatat tgctttctca tatcgatgcg gcaacggagc
     1201 gttttaatgc actattattg aatgactact caataaaaga aaaactcgct aagactgaga
     1261 atgcaatttc gactgtcgat aacattggct ggtttggaac aacatggctt gtttttacta
     1321 cagtagcacc cgaggcaaaa acgcattacc ttgcgctatt agagctgcag aggagcgaag
     1381 caaaagctac tatagccgcg ctatctaaac cactcgaaga cctaaaagaa ggacagtatc
     1441 atctcgtaat agattattta aaagagaatt ctgaaaacgc tgcattgctt tcaacagagg
     1501 tttcgcatta caaaaaaact ttttggctgt ggcagtcttg gcccgaaggt ttgattaaat
     1561 tagataaata tttatcagaa agcgtgccaa acgttaggat ccatcttcct caagaggaga
     1621 cagtaacgcc tgctctgctt tttgcagccg cacaaaaacc caatgaaccc aatgaaccca
     1681 aagaaatgta tgattataaa gtggaagcgg aacttcaact tgcaaatgca ctgaaactat
     1741 ctgaagctga agccatagct gcatctcaat ctaaaatagg ccctaaaaca atagcagggt
     1801 gggaattaac agatgttcca gaaataggaa attgttttta cgaagccgta gcgcttcaat
     1861 tgcagaaaaa caatttccct attgcaaaga cattcccagg aacaaggcca gaagatggtg
     1921 gaactgctct cgtaaatatc ctgcgtcgtc gaagtacgct agaatttaga gatcgcgact
     1981 gggctggaga ggaagaaata ttagctctcg cgaaagaact taaaattgtg attgccattg
     2041 tcgatacgag atcagaaaat cccgaattcc aatactattt tgcgaactgg aacggaacca
     2101 tgggcaccac gcgtaataaa acagaaatac cgcagggtca aatggttgca gagctcgctt
     2161 atacaggaaa tcattatttg tctgtaagat caaagccaag cagcccaaca tctccaagac
     2221 agtaatacct acaggcataa gctgaattta cttaaacagc gtttttacaa gatatacccg
     2281 attttttggg ttgagggtga ccttcatttt gaggagggat catgcgttgt ttataaatac
     2341 taaacaagtg ccgtaaagac tctgggacac ggttacccca tactacagga tcaggcgatt
     2401 tgggtatcag ctctttgtat tctgttctat ccgtcaaata accaagaatt atttgcaaag
     2461 gcttcgtcga taaccgcggc tccgaagcct tttctaagac atcactcggt gcctgagttt
     2521 ccaaaatctt aaaaatactt gcaagcatct cgaccctctc gttctggaac cacgccagac
     2581 tcgtcagcaa tccctgccat cttaattgca acccctctat cgtatctgga accgttgtta
     2641 aaaatgggat tgtgtccaaa gaaagctctt ctaattcaaa acaacccccg accgcatatg
     2701 gaatct
//
LOCUS       DAORVT010000155         2706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_35405_length_2706_cov_2.652584, whole genome shotgun sequence.
ACCESSION   DAORVT010000155 DAORVT010000000
VERSION     DAORVT010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35405_length_2706_cov_2.652584"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PK389_03865"
     CDS             <1..531
                     /locus_tag="PK389_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HQY22871.1"
                     /translation="QVLENIAHVEVIRIHTRVPLFDSNRITDQMIQALKINKAVYVAL
                     HTNHPSELTERAQKACARFVDAGIPMLSQTVLLKGINDTPEIMGELMRGLVKNRIKPY
                     YLHHGDLAEGTAHFRTTVKEGQALMKALRRQYSGLCLPTYVLDIPGGFGKVPIGHTYF
                     ECGKVEDYQGNLHSYD"
     gene            518..1330
                     /locus_tag="PK389_03870"
     CDS             518..1330
                     /locus_tag="PK389_03870"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012534276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HQY22872.1"
                     /translation="MLMIRPFLKWPGGKYRLVDRIQGLLPQGKQLIEPFVGGGAVFLN
                     TDFERYLLNDANSDLITLYQTLQKKGNAFIAVCRSFFIKKYNTEKHYYKFRTEFNAST
                     DPILRSALFLYLNRHGYNGLCRYNAGKGEFNVPFGSYKKPYFPEKEMLHFCEVSKRAV
                     FVCEDFTETLKKAKKGSVIYCDPPYVPLTATANFTRYQSQGFSLAEQQVLVDWAKKCA
                     KKGIPVLLSNHSNDFTRNIYAGAIIEEFDVRRLISCKIALRRPVLELLALYL"
     gene            complement(1367..2638)
                     /locus_tag="PK389_03875"
     CDS             complement(1367..2638)
                     /locus_tag="PK389_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HQY22873.1"
                     /translation="MNSIDLSDELLFSLLVFAVLLCAFCAASETGILSLNRFRLKHLV
                     KTEPYARRITKLLQHPDRLLGVILIGSTFATAISASIANQIAEHFWGELGVIISPIIV
                     TLFLLVFAEVMPKTVAALRPEQTSRFVSLPLQVMQWLLYPLVFAAAAISSTLLGLFGL
                     KTSTKTTDAVTADELRTIVSEAGNFIPSNHQSMLLSILDLENVRVEHIMIPRNEVVGI
                     DLDDDKNEIISKLKDLQHTLLPVYRSDLDNVQGILHTRNIVKILAQRELNEQGLLSVM
                     EEPYFVPEGTSLHTQLLNFQKNKRRMGLIVDEYGDVLGLVTLEDILEEIVGQFTTEAD
                     TEIPEVQPQEDGTFLIDGGISVRDLNRLQQWELPTDGPTTLNGLIIERLEFIPPEGTC
                     LLIAGHPIEVVQLKENTIKIAKISPKLMKEK"
BASE COUNT          797 a          500 c          579 g          830 t
ORIGIN      
        1 caggtcttag agaatatagc gcatgtcgag gtgattagaa ttcatacgag agtgcccctt
       61 tttgattcaa acagaattac agatcaaatg atccaggcgt taaaaatcaa taaagccgtt
      121 tatgttgcgc tgcatacgaa tcatcccagt gaattaacag aacgtgcaca aaaagcatgc
      181 gcacggtttg tagatgctgg aatccccatg ttatcccaga ccgtcttgtt aaaagggatc
      241 aatgatacac cagaaatcat gggcgaatta atgcgtggac tggttaaaaa tcgaataaag
      301 ccgtattatt tgcatcatgg cgatttagca gaagggacag cgcattttcg aaccacagtt
      361 aaagaagggc aagcattgat gaaagcgctt cgccgtcaat attcaggatt gtgtctacct
      421 acttatgtat tagatattcc cgggggtttt ggtaaagttc caatcggaca cacttatttt
      481 gaatgtggca aagtagaaga ttaccaagga aatttgcatt cttatgatta gacctttttt
      541 aaaatggcct ggtggaaaat accgattagt cgatcggatc caaggtttat taccccaagg
      601 gaaacaatta attgagccat tcgtgggggg gggagctgtt tttttgaata cggattttga
      661 acgctatctc ttgaacgatg caaattccga tttgattacc ttgtatcaaa cgttacaaaa
      721 aaaaggcaac gcttttatag cagtatgtcg ttcgtttttt atcaaaaaat ataatactga
      781 aaaacactat tataagtttc gaacagagtt taatgccagc actgatccaa tattacgttc
      841 ggccttattt ttatatttaa accgccatgg ctacaatgga ttgtgtcgtt ataatgcggg
      901 caagggtgaa tttaatgtgc catttggttc ttataagaag ccttattttc ccgaaaaaga
      961 gatgcttcat ttttgtgaag tctcaaaacg cgctgttttt gtgtgtgagg attttacgga
     1021 aacgcttaaa aaagctaaaa aagggagtgt catttactgt gatccgcctt atgtgccgct
     1081 cacggcgacg gcaaatttta cacgctatca atctcaagga tttagtttag cggagcaaca
     1141 ggtcttggta gattgggcaa aaaaatgtgc gaagaaaggc attcctgttt tgttgtcgaa
     1201 tcatagtaac gattttacgc ggaatattta tgcaggggca attattgagg aattcgatgt
     1261 gcgtcgatta attagttgta aaattgcctt aaggcgccct gttttggagc ttttggcact
     1321 gtatctttga ttcgttgact tttatccgcg ttcagacttg tttcctttat ttttctttca
     1381 ttaatttagg cgaaattttt gcaattttaa tggtgttttc tttcagttgt acgacttcaa
     1441 tgggatgtcc ggcgatcaag agacacgtgc cttcaggcgg gatgaattct aatctttcga
     1501 taatcaagcc attcaatgtg gtagggccat cggttggtaa ttcccattgt tgcaaacgat
     1561 taagatcgcg aacactgatc ccgccatcga ttaaaaaggt gccgtcttct tgcggttgca
     1621 cttcgggtat ttcggtgtca gcttccgtgg tgaattgtcc aacaatttct tctaggatat
     1681 cttccagcgt gacaagccct aaaacatcgc catattcatc aacaatcaat cccattcgac
     1741 gtttgttttt ttggaaattt aagagctgag tgtgcaaaga agttccttca ggtacgaaat
     1801 agggttcttc cattacgctt aataaccctt gttcgtttaa ttctcgttgc gctagaattt
     1861 ttacgatatt tcgggtatgt aagatccctt gaacattatc taagtcagaa cggtagacag
     1921 gtaaaagagt atgttgcaaa tcttttagtt tggaaataat ttcattttta tcatcatcca
     1981 aatcgattcc gacgacttca tttcgaggga tcataatatg ttcaacgcga acattttcaa
     2041 gatctaaaat actgagtaac atgctttgat gattgcttgg aataaaattg cccgcttcac
     2101 tcacaattgt tcggagttcg tcggctgtga cggcatccgt cgtttttgta gaagttttaa
     2161 gtccaaataa acccaataag gtactagaaa tcgctgcggc cgcaaaaacc agtgggtata
     2221 ataaccattg catcacttgc agcggtagag atacaaaacg cgaggtttgt tcaggtctta
     2281 aggccgctac cgtttttggc atgacttctg caaaaaccaa taaaaaaagt gtaacaataa
     2341 tgggtgaaat aataacgccg agttcgcccc agaagtgttc ggcgatttga ttggcaattg
     2401 aagctgagat ggctgttgca aaagtacttc caataagaat aacaccgagc agtcgatcgg
     2461 ggtgttgcag caatttggta atgcgacgtg cgtagggttc tgttttcact aaatgtttta
     2521 atctaaagcg gttaagcgat aaaatacccg tttcactggc tgcacaaaaa gcgcaaagca
     2581 aaacggcaaa taccaataga gaaaataaaa gttcatcaga taagtcaatg ctattcaagg
     2641 ttatttacat cctttttcat ctactatgga tcccagagtc tatcgaacaa caggtgattg
     2701 tcactc
//
LOCUS       DAORVT010000156         2705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_35425_length_2705_cov_2.266038, whole genome shotgun sequence.
ACCESSION   DAORVT010000156 DAORVT010000000
VERSION     DAORVT010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35425_length_2705_cov_2.266038"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PK389_03880"
     CDS             <1..223
                     /locus_tag="PK389_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020308056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin-binding protein (FBP)"
                     /protein_id="HQY22874.1"
                     /translation="IGRPSQISLLIGNNVGDLKALTDHYLPKSAIDKATFRMSELLKQ
                     RFGTKEPDTKSTAIALPQASSDDSLEDFF"
     gene            260..2287
                     /locus_tag="PK389_03885"
     CDS             260..2287
                     /locus_tag="PK389_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011051591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQY22875.1"
                     /translation="MGSIDFVSPDEIKVLLDIEAPDSVALNTGTPRPFPRINGYVLIS
                     SDDGYLVAQVEWITVERSQYPKRKGMQDFGLIDLPYPLRKMSLNPLGVLQYDGSKDKD
                     GDDRYIFKRGVEAFPSVGEAVLLPTQKQLRSIVESGDNRHIKIGISPLAANAEVCIDP
                     DRLFGRHLAVLGNTGSGKSCTVAGLIRWSLDAAKKECGKEKEPNARFIVLDPNGEYAH
                     AFKDDKKVRVYAVEPATDIKQLEVPLWFWNSAEWSAFTQASSKTQRPTLIQSLRTVRN
                     GQIETALTPSHEMRRFLRTIVSIIQIDRNTGKPWAGFAQSKGFMEKIKKWNEGLLDND
                     SFSAEEKSAMENLRAKISNFELARSGNYPTWDFTKPEIDEFLQLAKAAHSAFGGSDTD
                     VLPIDADIPRPFTGDTLLRSVEANAEMLNVSEYVETMLMRIRTMLSDTRMKTITGNAE
                     GVTLDSWLSDYIGSDGASNGTVTVIDLSLVPSEVVHIITAVIARMTFESLQRFRRMDK
                     KKKTLPTVLVLEEAHTFVKRYKDDAENHDAATMCCQVFEKIAREGRKFGLGLVLSSQR
                     PSELSPTVLSQCNSFLLHRISNDRDQELVQKLVPDNLRGLLRDLPSLPSRYAILLGWA
                     SELPVLVQINSLPEEQRPKSEDPDFWDVWSGKTERKVTWKEITDDWQNANH"
BASE COUNT          802 a          530 c          601 g          772 t
ORIGIN      
        1 aataggtcgc ccttcacaaa ttagcctgtt gattggcaac aacgtgggcg atctaaaagc
       61 attgactgat cactacttgc caaaatctgc gattgataaa gctactttcc gtatgagtga
      121 gctacttaag caacgttttg gtactaaaga gccagatact aaaagcactg caattgcact
      181 gccgcaagca agttctgatg attctttaga ggattttttc taatgggcct taccacttgc
      241 ttacgctgat tctcttcgaa ttggcagtat agactttgta tcacccgatg aaataaaagt
      301 attattggat attgaagctc ccgattctgt ggcattgaat acaggtactc cgcgcccgtt
      361 tccgcgcatt aacggttatg tacttatatc cagcgatgac ggttatttgg tcgcacaagt
      421 ggagtggatt acggtagaac gttcccagta tcctaaacgt aaagggatgc aagactttgg
      481 attgatagac ttgccctatc cattgcgaaa aatgagccta aacccattgg gtgttttgca
      541 atatgatggt agcaaagata aggatggtga tgatcgctat atttttaaac gaggcgttga
      601 ggctttccca tcagtgggtg aggccgtact gctacccaca caaaaacaac tgcgttctat
      661 tgtcgaatct ggcgacaatc gccatataaa aataggaatt agtccattgg cggctaatgc
      721 cgaggtttgt attgatcctg accgattatt tggtcgtcat cttgctgtgt tgggtaatac
      781 aggcagtggt aaatcatgca cagtagccgg tttaattcga tggtcactgg atgctgcaaa
      841 aaaagaatgt ggcaaagaaa aagaacccaa tgcccgtttt attgtactcg accccaatgg
      901 tgagtatgct catgcgttta aggatgataa gaaagtcaga gtttatgcgg ttgaaccggc
      961 aaccgatata aagcaattgg aagtaccttt gtggttttgg aatagcgcag agtggagtgc
     1021 ttttacacag gcaagtagca agacacaaag accaactttg attcaatcac ttagaacagt
     1081 acgaaatgga cagattgaaa ctgcgcttac acctagtcat gaaatgcgac gatttttacg
     1141 cactatcgta agcataattc agattgatcg caatactggc aagccttggg cgggttttgc
     1201 tcaatcgaaa ggttttatgg aaaaaatcaa gaaatggaat gagggtttgt tggacaacga
     1261 ctcattttca gcagaagaaa aaagtgcgat ggaaaatcta cgggcaaaaa tttccaattt
     1321 tgaacttgcc cgaagtggta attatcccac ttgggatttt accaagccag aaatagatga
     1381 atttctgcaa ttggcaaaag cggcacattc tgcttttggg gggagtgata cagatgttct
     1441 tcctattgat gcagatattc caagaccatt tacaggggat acgcttttgc gaagtgtaga
     1501 agcaaatgcc gaaatgttaa atgtatctga atatgttgaa acaatgctta tgcgtatacg
     1561 tacaatgctt tctgacaccc gtatgaaaac gattacagga aatgctgaag gtgttacatt
     1621 ggatagctgg ctcagtgact atattggtag tgatggagca tccaatggta ctgttacggt
     1681 aattgacctt tcccttgtcc cttccgaagt tgttcatatc atcaccgccg tgattgctcg
     1741 tatgactttt gagtctttac aacggtttcg cagaatggat aagaaaaaga aaaccttgcc
     1801 aaccgttctg gtcttagaag aagcacatac ttttgtcaaa cgctataagg atgacgctga
     1861 aaaccatgat gctgcgacta tgtgctgtca ggtgtttgaa aaaatagccc gcgaaggtcg
     1921 aaaatttggt ttgggattag tattgtcttc tcagcgtcct tccgagttat caccaactgt
     1981 tttatctcaa tgcaatagtt ttctgcttca tcgtatcagt aatgatcgtg atcaagaatt
     2041 agtacaaaaa cttgtacctg ataatctgcg tggactatta cgcgacttac catcactacc
     2101 ttcacgatat gctattttgc tagggtgggc atctgaacta cccgtgctgg tacaaataaa
     2161 ttcattgcca gaagagcagc gcccaaaatc ggaagatcca gatttctggg atgtttggtc
     2221 tggtaaaaca gaacgaaaag tgacttggaa agaaattacc gatgattggc agaatgctaa
     2281 tcattaagca aaatgataag tgtctttaat ttttcaagct attttatagc aaagcacgaa
     2341 tgacattgag caacctgaca atcgccacag gatttttgtc cctgaccgta tgttggccca
     2401 ttcctaactt tattcatcat cataaagcgg tcagtcgcga ccggaagaaa ttggccgaat
     2461 ccagccaatc aatgccacag attgaataga gactctagtt attgatccac caatcatacc
     2521 aatcttggcc agcgattatg tatccagaca tattcaggtg gtgcatcgac agtcatcaca
     2581 taaacccgag tttctttgtc tctttctgcc aataatgggt taaatcccat aaaggtacga
     2641 aactaccaat aggatttttg agcatagatt gagcggcccg gattctttca tctgtcatga
     2701 gggtt
//
LOCUS       DAORVT010000157         2698 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_35579_length_2698_cov_2.865305, whole genome shotgun sequence.
ACCESSION   DAORVT010000157 DAORVT010000000
VERSION     DAORVT010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35579_length_2698_cov_2.865305"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="PK389_03890"
     CDS             complement(<1..1008)
                     /locus_tag="PK389_03890"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HQY22876.1"
                     /translation="MISDFLNLVSGVTDMPWWGYVVVTLVMTHITIASITIYLHRHSA
                     HRALELHPIVSHFFRFWLWLTTGMVTKEWTAVHRKHHAKCETKADPHSPVTVGIKKVL
                     LEGSELYRTEAKNQETLDRYGYGTPDDWVERNVYTKHSAKGVALMLIIDVILFGPIGI
                     TIWAVQMLWAPIFAAGIINGVGHYWGYRNFQAEDASRNIVPWGILIGGEELHNNHHAF
                     ATSARLSNKWYEFDIGWLYICILQVFGLAKVKKTAPKLRMNKQKDCCDLDTLQAVISH
                     RYEVLAKYTKSLQQAFTKEMDGLKGAIAEYDIDKSTLKRWVMADSRTLQAHEKEKLSQ
                     VL"
     gene            complement(1358..1804)
                     /locus_tag="PK389_03895"
     CDS             complement(1358..1804)
                     /locus_tag="PK389_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HQY22877.1"
                     /translation="MDLILWRHAEAEEGFPDSARQLTVRGRAQAKQMAIWLRARLPEN
                     TEVLVSPTSRTQQTASTLEFDFKTIDTIAPGATVQDILTAADWPNKQGAVIIVGHQPT
                     LGEVIHHLIRDVPTRLKVKKSSVWWIKKDKNDIEPLLKVVIYPDVL"
     gene            complement(1832..2143)
                     /locus_tag="PK389_03900"
     CDS             complement(1832..2143)
                     /locus_tag="PK389_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HQY22878.1"
                     /translation="MGWYHYDNSNNLVLKLYIQPGAKNTAVVGLHAEALKIKLAVLPI
                     NGKANKYLMRFLADCFQVPISQIEIKEGDKSRQKLLLIRKSGLNPDVLYGEKNNSLIN
                     V"
     gene            complement(2163..2696)
                     /locus_tag="PK389_03905"
     CDS             complement(2163..2696)
                     /locus_tag="PK389_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HQY22879.1"
                     /translation="MGFFSLALLLRFYFQFFRLPYRHPISQFLVAVTGFMVRPVRRFV
                     PGWGGVDLSSLLLAWLLECAILFGTFMLRGYDFSVNMEASLPGVLLLGTVEIVKMSLY
                     IVLVAVILQAILSWVNPHTPLAPLLDGLTRPFLNIFRNRIPLVANVDLSPLFVLLVIQ
                     VLLMIIMEVHREIATML"
BASE COUNT          868 a          621 c          438 g          771 t
ORIGIN      
        1 caaaacttga ctcaattttt ccttttcatg tgcttgcaat gttctggaat cagccattac
       61 ccaacgcttt aatgttgatt tatcaatatc gtactctgca atagctcctt tcaacccatc
      121 catttctttt gtaaaagctt gttgcaacga tttagtatat tttgcaagta cttcgtagcg
      181 gtgagagata actgcctgta atgtatctaa gtcacagcaa tctttttgtt tattcattcg
      241 tagcttaggt gccgttttct tgacttttgc caaaccgaac acttgcaaaa tacaaatata
      301 aagccaacca atgtcaaatt cataccactt gttagacaat cgagcagatg tcgcaaaagc
      361 atgatgattg ttatgcaatt cctcgccccc tatcaaaata ccccaaggaa ctatgtttct
      421 actcgcatct tccgcttgaa aattacggta accccagtaa tgcccaacac cattaataat
      481 gccagcagca aaaattggtg cccataacat ttgtactgcc cagatagtaa taccaattgg
      541 accaaataga atcacatcaa tgatcagcat taatgcaaca ccttttgcac tatgcttagt
      601 atagacatta cgttccaccc aatcatcagg agtaccatat ccatatcgat ccaaggtttc
      661 ttggtttttt gcttccgttc tatatagctc agagccttcc agcaacactt ttttgatgcc
      721 aacagtcacc gggctatgcg ggtcagcttt agtttcgcac ttggcatgat gtttacggtg
      781 gactgctgtc cactcttttg tcaccattcc tgtcgtgagc cacagccaaa aacgaaaaaa
      841 atgactcact attggatgca attcaagcgc acggtgcgca gaatggcggt gtagataaat
      901 cgtgatactc gcaattgtta tatgcgtcat aaccagcgta acgacaacat acccccacca
      961 aggcatatct gtaacaccag acactaaatt aagaaaatcc gaaatcattt cacgaaaatc
     1021 cttttaaaga attcttaata cttaataaac tgaccccact ttgtataaac caaagccacc
     1081 agagtcatcc ccataaacta catttactct acaagaactc tgtagaaact ctaagctatt
     1141 attgagtatg cacaataata ttttttcaat ccgtttatca taaataacgc tttatcttta
     1201 tgttaatttt tatgctcatt cattatgaat aaaatcactc gcttacttac aagctgataa
     1261 attttaaccg aactattcta cattgatctt tctctttata gaaaaaatcg ttaggattaa
     1321 aaatcactga ttaaatgtaa aatccagtga atatgaatta caacacgtct ggatatatca
     1381 ccacttttag cagcggctcg atatcatttt tatctttttt tatccaccat actgaagatt
     1441 tcttcacttt cagcctggtt ggcacatcac gaatcaaatg atgaatgact tctcctaacg
     1501 ttggctgatg accaacgatt attaccgcgc cttgcttatt tggccaatca gccgccgtaa
     1561 gaatatcttg aacagttgca cctggcgcta tggtatcgat ggttttaaaa tcaaactcaa
     1621 gcgttgaagc ggtttgctgc gtacgactgg ttggactaac aagcacttct gtgttttccg
     1681 gtagcctagc tctaagccag atagccattt gttttgcctg agctctcccc cttacagtta
     1741 actgtctcgc agaatcagga aaaccttctt cagcttcagc atgacgccat aaaattagat
     1801 ccataaatta gttacaaaaa tatgctaatg cctatacatt tatcaagctg ttatttttct
     1861 caccatacag tacatcagga tttaacccag atttgcgaat cagcaataat ttttgcctgg
     1921 atttatcgcc ctcctttatt tcaatctgac taataggaac ttgaaagcaa tccgcaagaa
     1981 acctcattag atatttattt gcctttccat taatcggcaa aaccgcaagc tttatcttca
     2041 aagcttcagc atgcaatcca acaacagcag tatttttagc accgggctgt atatacaact
     2101 tcagaactaa attgtttgaa ttgtcgtaat gataccaacc cattgtagtt taaatactca
     2161 aatcaaagca ttgtagcaat ttcccgatgc acttccataa ttatcatcaa taagacttga
     2221 atcacaagaa gaacaaaaag gggcgacaaa tccacattcg ccacgagtgg aatacgatta
     2281 cgaaagatat tcaaaaacgg gcgagtaagg ccatccaata aaggtgccaa tggggtatgc
     2341 ggattaaccc atgacaagat agcttgcaga ataacggcaa ccaaaacgat gtacagcgac
     2401 atcttaacaa tttccaccgt acccaataac agcacaccgg gcagcgatgc ttccatatta
     2461 acgctaaaat cataacctcg tagcataaag gtgccaaaca aaatagcgca ctccagtaac
     2521 catgctagta ataatgatga cagatcgacg cctccccacc cgggaacaaa tcttcgcacc
     2581 ggtcgcacca tgaaaccggt aacggcaaca agaaattgtg agatcggatg acggtatgga
     2641 agacgaaaaa actgaaaata aaaacgcagc agcaaagcta atgaaaagaa accgatta
//
LOCUS       DAORVT010000158         2692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_35744_length_2692_cov_3.218051, whole genome shotgun sequence.
ACCESSION   DAORVT010000158 DAORVT010000000
VERSION     DAORVT010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35744_length_2692_cov_3.218051"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..779
                     /locus_tag="PK389_03910"
     CDS             168..779
                     /locus_tag="PK389_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22880.1"
                     /translation="MSTIAATQNISFPGFDNRSSTGSGSEEISNLFNKARKGLRLIRP
                     AHQQYQPQVSETLEDRLDHTKALCKRKTAEVAMYLSEDFRKGFFLQIDNLIDAENWEE
                     DDKPITEASFTTLLRMLILINPDLRPGLGSTSQGNIIAAWTKDKDQLTIECLPEDKVR
                     WVLSRQFNGIRESAAGEILLTRLLAALAPYSPGNWFDNDPQEK"
     gene            754..1212
                     /locus_tag="PK389_03915"
     CDS             754..1212
                     /locus_tag="PK389_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22881.1"
                     /translation="MTMTLKKNDVIPNDNHVMRYVPWNRLRKDENDNVIGILGEAFAL
                     RDGEEGISSTWLEYFPDNQISQITQAVHKNRKCLKVTPKSGFAIGKVEDILSECNKNN
                     KSKKIRVVYMPNNCNKAHIEIKPMPCNDALLLELLASDAWSQLILNRDIP"
     gene            complement(1321..1794)
                     /locus_tag="PK389_03920"
     CDS             complement(1321..1794)
                     /locus_tag="PK389_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22882.1"
                     /translation="MTTPSREELLSQKVIYSRSSDKNYLEIVECLKSEEGFPLPKWHR
                     HTWRDINTYFIYHESFQVDGYPQMHDEAAKTFYITEIHNPKSLGSFDKPISHGNGNWI
                     MTIKDTGNAQKTQSKIEIRSNQNLLSDYPPASYTPDPNASLSQRLYKVERIDHCF"
     gene            2263..>2692
                     /locus_tag="PK389_03925"
     CDS             2263..>2692
                     /locus_tag="PK389_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HQY22883.1"
                     /translation="MNTEDFRPEKEIKIFYPPQRVLMGPGPSDTHPRVLNAMARPTLG
                     HLDPVFTEMMEEIKGLMRYAFQTKNRMTFPVSGPGSVGMEMCFVNMIEPGDKVIVCRN
                     GVFGGRMIENVERHGGVAVVVDDKWGEPVDPQKVEDALKKN"
BASE COUNT          827 a          490 c          599 g          776 t
ORIGIN      
        1 ataaaaatta attcaaatgg gtgtgtggct agtattgata tttataataa tgaaaaagta
       61 ccacttgatg acaaaggtct aatgtcaaga cttgaggaag ctagagaact aaagaatctt
      121 gtatttaaac agtgtataac atcgaaaaca gaggaattat tcaactgatg tcgactatag
      181 ctgcgaccca aaatatttct ttccctggat ttgataatcg gtcctctaca ggaagcggta
      241 gtgaagagat atcgaatctg tttaacaagg cacgcaaagg attacgtctt attaggccag
      301 cgcaccaaca atatcaaccc caagtatcag agacacttga agataggctc gatcacacca
      361 aagccctttg caaaagaaag acagcagagg ttgccatgta tttgtcggag gattttagga
      421 aaggcttttt tctacagata gataatttaa tagatgcaga gaattgggaa gaagatgata
      481 agcccattac tgaagcatca tttacgacat tattacgaat gcttatcctc ataaacccgg
      541 atctcagacc tggactcggt tccactagtc aaggtaatat tatcgcggca tggacaaaag
      601 acaaagacca gctgacaata gaatgtttgc ctgaagataa ggttcgctgg gttctaagtc
      661 gtcagttcaa tggaattcga gagagcgctg ctggggaaat cctattaact cgcctacttg
      721 ctgccttggc tccttatagc ccaggtaatt ggtttgacaa tgaccctcaa gaaaaatgat
      781 gtaattccaa atgacaacca tgtcatgcga tatgttccat ggaatcgact ccgaaaagat
      841 gagaatgata atgttattgg tatccttggt gaagcttttg ctttgcgtga tggcgaagaa
      901 ggtatttcat caacctggct cgagtatttc ccagataatc aaatatccca gataacccaa
      961 gcagttcata aaaatcgtaa atgccttaaa gttacgccaa agagtggctt tgccataggc
     1021 aaagtagaag atatcttgtc ggaatgtaat aaaaataaca aaagtaagaa aattcgagtt
     1081 gtttatatgc caaataactg taataaagcc catattgaaa tcaaaccaat gccttgtaat
     1141 gatgcattat tgcttgaact cttggcctcg gacgcttggt cacagttaat attaaacaga
     1201 gatattccat aagtgcggta ggttggggtg acgataggaa ccccaacatc acaatgccgc
     1261 ataaattgtt gagattcgtc ggctcatctc aatctgcggc ttattaaaat cgcgtatcaa
     1321 tcaaaaacaa tgatctattc tttctacttt atataagcgc tgggagagtg aagcgttagg
     1381 atccggtgta taagaagccg gtgggtaatc actgagaaga ttttgattac tacgaatttc
     1441 gattttggat tgggtctttt gcgcattacc ggtatctttg atcgtcatga tccagtttcc
     1501 attgccgtgg gatatcggtt tgtcgaagct tcctaaagac ttaggattat gaatctcagt
     1561 aatgtagaaa gtcttggcag cttcgtcgtg catttgcggg tatccatcga cttggaaaga
     1621 ttcgtggtag ataaaatagg tattgatatc gcgccaggta tgacgatgcc atttcggtaa
     1681 aggaaatcct tcctcgcttt ttagacattc cacaatttcc agataatttt tgtcactgct
     1741 gcggctataa ataactttct gactgagtaa ttcttctcgt gagggtgttg tcatgcaagc
     1801 gattaacatg agtacacaga gcagaagcgg gatcgattta tttagagaat ggcgcataag
     1861 atggaaagct tagaatttta atatttaggg taaatcgaat ggtatcagaa tcattttgta
     1921 ttgcacatga gcgaaggagg attagatagt gcagaccgta tcctctgaat gaaccagtat
     1981 ctgcaaattt tctgttttat ttaagcaaag aaatcgttgt agtcttagtg ctctccatat
     2041 tttactagtg tcttgcggga ccagataatc tcgatgtgta acgtaaaaac tgccagctaa
     2101 cactaatagc tcaaaatttc agattgttat gcgaatttct ttgcttttcc ataaggataa
     2161 tttattgagc acacctttgt ttatagtaaa ctctcgtttc acttgttaga atcaacattt
     2221 ttcaaaaata gatcagatta cattattaac ttaggattgt tcatgaatac cgaagatttt
     2281 cgcccagaaa aagaaatcaa aatattttac ccaccccagc gcgttctgat gggaccggga
     2341 ccatcggata cacatccgcg tgtgctgaat gccatggcgc gcccgacctt aggtcacttg
     2401 gatcctgttt ttaccgaaat gatggaagag attaaggggt tgatgcgcta tgcattccaa
     2461 accaaaaacc ggatgacctt tccggtttcc ggaccgggat ctgtcgggat ggaaatgtgc
     2521 tttgtcaaca tgatcgaacc cggtgataaa gtgattgtgt gtcgcaatgg cgtatttggc
     2581 gggcgcatga ttgaaaacgt tgaacgtcat ggtggtgttg cggtggtagt ggatgataaa
     2641 tggggtgaac cggttgatcc gcagaaagtg gaagatgcgc tgaaaaagaa cc
//
LOCUS       DAORVT010000159         2655 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_36625_length_2655_cov_4.499231, whole genome shotgun sequence.
ACCESSION   DAORVT010000159 DAORVT010000000
VERSION     DAORVT010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36625_length_2655_cov_4.499231"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /gene="mutL"
                     /locus_tag="PK389_03930"
     CDS             complement(<1..1018)
                     /gene="mutL"
                     /locus_tag="PK389_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009721938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HQY22884.1"
                     /translation="MIVEEKLEKNSERKLEQKRIHQLPVHLANQIAAGEVVERPASVL
                     KELLENSLDAKSTEIEITIERGGTKLIQVQDNGLGICKEDLPLAIASHATSKIASLSD
                     LEGVKSYGFRGEALASISSVSRLELISAVEEQSCGWSIQLEGRDKPPSLQPVAKRPGT
                     LIAVRDLFYNTPARRKFLKSEKTEAAYLEEIFKRIALSQPAVAFKFQQNDGVQKRLPI
                     CRSLEAHIRRVAQLCGKRFVEEAYYIEAEANGLKLTGWLGSENTMRSQADLQYFYVNG
                     RIVRDKIVMHAVRQAYQSINSLGRFPAYVLYFECDATAVDVNVHPTKHEVRFREARTV
                     HAFLS"
     gene            complement(1015..1443)
                     /gene="tsaE"
                     /locus_tag="PK389_03935"
     CDS             complement(1015..1443)
                     /gene="tsaE"
                     /locus_tag="PK389_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HQY22885.1"
                     /translation="MILPDALATEAFGKQLAAIVKPPTVIFLEGELGAGKTTLVRGFL
                     RGFGYQDSVKSPTFTLVEPYQFETCRIYHFDLYRLNDPEELELMGVRDYFTEDAIILV
                     EWPERGLGFLPKADLILELSLIPEGRGLKIKKLIKRDKSK"
     gene            complement(1467..1712)
                     /locus_tag="PK389_03940"
                     /pseudo
     CDS             complement(1467..1712)
                     /locus_tag="PK389_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008927313.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
     gene            1925..2644
                     /locus_tag="PK389_03945"
     CDS             1925..2644
                     /locus_tag="PK389_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22886.1"
                     /translation="MSSEIEQLQQNIDNTIRDATNLLEQAKQHLKTLETKGYIYELNS
                     LPIELGQEVIQFILFCRLIDTKKAELEKLKTEITPENINAPNPQETNFSQDLPKSEES
                     TSKKESKNKEESTMNEEENEDWAAEADAEEKEKIARGEKLFDPVEFAKSIQLDINKPK
                     INTKSVNAQYQELANSIKRLEADFPKNKESSIQKIVDKETAIKMLFSPAMLYLKKRQA
                     EAKEQTPVSDTPTAPSSDPKR"
BASE COUNT          858 a          602 c          475 g          720 t
ORIGIN      
        1 aagataaaaa agcatgtact gtacgagcct ctctaaaacg cacctcgtgc tttgtaggat
       61 gcacattaac atccactgca gtcgcatcac attcaaaata caatacatac gctggaaatc
      121 gacctaaaga attgatagac tgataggctt gtcgcacagc gtgcatcaca attttgtcgc
      181 gcacaattcg gccattaaca taaaaatatt gtaaatctgc ttggctacgc atcgtgtttt
      241 cagaacctaa ccagcctgtt aattttaaac catttgcctc tgcttcaata taataagcct
      301 cttcaacaaa acgtttgcca cataactgcg ccacacgtcg aatatgtgct tctaaactcc
      361 gacatattgg taaccgtttt tgtacgccat cattctgttg aaacttaaaa gccaccgccg
      421 gttgacttaa agcaatacgt ttaaatattt cttctaaata cgccgcttct gttttttccg
      481 actttaaaaa tttacgtcgc gcaggcgtat tataaaaaag atcgcgaact gcaattaaag
      541 tgcctgggcg ttttgcaaca ggctgtaagc tcggcggttt atcgcgaccc tctaattgaa
      601 tagaccatcc acaagattgt tcttcaaccg cagaaatcaa ttctaaacgt gataccgaac
      661 taatgcttgc taatgcttct ccgcgaaacc cataagattt tacaccttct aaatccgaca
      721 aactggcaat tttactggtt gcatgcgatg caatagccag cggtaaatct tctttacaaa
      781 tacctaaccc attgtcttgc acttgaatta attttgtacc gccgcgttca atcgtaattt
      841 cgatctcggt gctttttgca tccaaactgt tttctaacaa ttctttcagc actgatgcag
      901 gtcgttcgac cacttcaccc gctgcaatct gattcgctaa atgtacaggc aattgatgaa
      961 tgcgtttttg ttctaacttt cgctctgaat ttttttctaa tttttcttct acgatcattt
     1021 tgatttatct ctttttatta attttttaat ttttaagccc ctgccttcag ggattaaact
     1081 taattctaaa atcaaatctg ctttaggtaa aaatcctaaa ccccgttcgg gccattccac
     1141 caaaataatg gcatcttcgg taaaataatc tcgaaccccc attaattcta attcttcggg
     1201 atcattcaaa cgatacaaat caaaatgata aatcctgcat gtttcaaatt gatacggttc
     1261 aaccaatgta aatgttgggc tttttacaga atcttgatat ccgaaacccc ttagaaatcc
     1321 gcgaacaagc gttgttttgc ccgcacctaa ttctccttct aaaaaaatca cagtgggtgg
     1381 tttaacgatt gcggcaagtt gtttaccaaa cgcttctgtc gcaagggcat ctggcaaaat
     1441 aatcgttgat tctgtctctg atgcgcttag ttcaagcacg tccgaagtcc ttcgaataaa
     1501 tcgcgcgcaa ggatcccgcg ctcaccacgt gtgctggctt tatcacctgc tgtagcatgt
     1561 gcgacaacgc ccaaacgtgc ggcatctaac aacgaaaggc cttgtgccac taaggccgct
     1621 tctcctgcca aacaacaggc gccacccatg ccatagtcac ccccgacaat taatacatgt
     1681 ccaaagtcac ctttatgggc atcgcgtgcc cgggggtgca agggttgacg gcaggcttct
     1741 aaagccagcg ttctgaaatc cgattgactc atgtttagat ccctttggtg ctcggtgatt
     1801 tgccctagga tatcgagtcg agacgaaggg gtcaaatcaa aggcaatggg gccatggggt
     1861 taaagtaatt cctcagtagg ccgaatatct atatcaataa tccgattatt aggagatggc
     1921 cgttatgagc tcagaaatag aacaattgca acagaacata gataacacta tccgcgatgc
     1981 gaccaattta cttgaacaag caaaacagca tttaaaaact ttagaaacca aaggatatat
     2041 ttatgaactg aactcccttc ccatcgaatt gggacaggaa gtcattcaat ttattctttt
     2101 ttgtagacta attgatacta aaaaggctga attagaaaaa ctaaaaacgg aaataacgcc
     2161 agaaaatata aatgctccaa atccacagga aacaaatttt tctcaggatt tacccaaatc
     2221 agaagaatcc acaagcaaaa aagaatcgaa aaacaaagaa gaatcgacca tgaatgaaga
     2281 agaaaacgaa gattgggctg ctgaagcaga tgcggaagag aaagaaaaaa tcgctagagg
     2341 cgaaaaacta tttgaccctg ttgaatttgc aaaatcaata caactggaca taaacaagcc
     2401 aaaaataaac accaaatctg ttaacgcaca atatcaagag ctggcaaatt caattaaacg
     2461 acttgaagcg gactttccaa aaaataagga aagctcaatt cagaaaatag tagacaaaga
     2521 aacagcaata aaaatgcttt tttctcccgc gatgctgtat ttgaaaaaaa gacaagctga
     2581 agcgaaagag caaacgcctg tatctgatac acccacagca ccctcctcag atcctaaaag
     2641 gtaatggggt caaat
//
LOCUS       DAORVT010000160         2647 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_36832_length_2647_cov_2.382716, whole genome shotgun sequence.
ACCESSION   DAORVT010000160 DAORVT010000000
VERSION     DAORVT010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36832_length_2647_cov_2.382716"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1781)
                     /gene="dnaK"
                     /locus_tag="PK389_03950"
                     /pseudo
     CDS             complement(1..1781)
                     /gene="dnaK"
                     /locus_tag="PK389_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637544.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
     gene            complement(1873..2412)
                     /gene="grpE"
                     /locus_tag="PK389_03955"
     CDS             complement(1873..2412)
                     /gene="grpE"
                     /locus_tag="PK389_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HQY22887.1"
                     /translation="MSNNDETAQTTETSENPVIPENPENLADLLEEARRKTAENWDLF
                     LRTRADADNIRRRATIDVENAHKYAVERLAKELLAVVDSLEHGLAAADTDSKALKEGM
                     ELTYKLLLDTLEKFGILAINPANEPFDPMKHEALTAQVKNDVEPNTVLMVVQKGFTLQ
                     DRLLRPARVIVSKVDASET"
BASE COUNT          661 a          690 c          481 g          815 t
ORIGIN      
        1 cgctagtgcc tccgtagcac gctcaatttc ggctttatcc gttcccttag ggcgtctttc
       61 aaagcatccg tagccttagt aatgctttct ttttcttccg cgctcacttt atcacccatt
      121 tctttgagtg atttttcagt tgcgtggatc atgctatcgg catggttacg cgcagaaacc
      181 aattcgtgga attttttatc ttcctctgca tgcgcttccg catctttcac catgcgttgg
      241 acttcttcct cattaagtcc actcgaggct ttaatcacaa ttttttgctg tttacccgac
      301 gccatgtctt ttgcggatac gcttaaaata ccattcgcat cgatatcaaa agcgacttcg
      361 atctgcggaa cgcctcgcgg tgccggtgga atatccgaca aatcaaactg tcctaagaac
      421 ttattcgcag ccgcttgctc gcgttcgcct tggaacaccc gaatggtcac cgccgtttgg
      481 ctatccgccg ccgtcgaaaa gacctgtgtc tttttggtcg gaatcgtcgt gtttttttca
      541 atcaattttg tcatcacgcc acccatggtt tctaaaccga gggagagcgg ggttacgtct
      601 aacaataaaa tgtctttaac atctccagat aaaacgccac cctgaatcgc agcaccaatg
      661 gcaactgctt cgtctggatt aacatctttt ctcggttctt gaccaaagaa atttttaaca
      721 atttcttgca ccttcggcat acgcgtctga ccaccaacta aaataacacc ctgaatatcg
      781 gtaatttttt tacctgcatc tttaagcgca attttgcaag gctcaatggt cttattcact
      841 aaatcttcta ccagagattc taattttgca cgggtaatct tcaagtttaa gtgtttagga
      901 ccactggcat ccgctgtaat ataaggcaaa ttcacatcca cttgttcgcg tgaagaaagt
      961 tcaactttgg ctttttctgc cgcttctttc aaacgctgca aggcaatcgg atcattcttc
     1021 aaatcaacat tgcattcttt tttaaattca gcaaccaaat aatccattac acggacatca
     1081 aaatcttcac cgcccaaaaa ggtatctcca ttggtcgcga gtacttcaaa ttgatgttcg
     1141 ccatcgactt ctgcaatttc gataatcgaa atatcaaaag taccaccacc caaatcgaat
     1201 acggcaataa ccatattgcc tttttgttta tccataccaa aggcaagcgc tgccgccgtt
     1261 ggctcgttga taatacgttt aacatctagc cctgcaatac gacccgcatc ttttgtggcc
     1321 tgacgctgag aatcattaaa ataagcagga acggtaatca ctgcagaatg cacttctctg
     1381 cctaaataat cttcggctgt ttttttcatt ttaactaaca cttcggcaga aatctgttgc
     1441 ggcgctaatt tctcatctga gccttgtagc caagcatcgc cgttatccgc acgcacaata
     1501 ccataaggca caatgtcttt atctttcgca accacgggat catcaaaacg acgaccaatc
     1561 aatcgcttaa ccgcataaaa agtttgcttt ggattggtga tacgttgatt tttagcagga
     1621 ccgccgacca atttttcttt gtctttcgta taggcaacaa tggaaggcgt tgttctcgca
     1681 ccttcgctgt tttcaataac ttttgcgacg ccattttcca taatcgcaat acaagaattg
     1741 gtggttccta aatcgattcc aataataact tctttactca tctctataat ctcctcattt
     1801 aaaacctggc tttcgcttat gagcgcaggc gctcaccttc tagtactata agttgggtca
     1861 aacttctgct tttcaagtct ccgaagcgtc tactttagaa acaattaccc gcgcaggccg
     1921 caacaagcga tcttgcaaag taaagccctt ttgaaccacc attaataccg tatttggctc
     1981 aacatcgttc ttgacttgcg ccgtcagtgc ctcatgcttc attggatcaa aaggctcgtt
     2041 cgctggatta atagctagta ttccaaattt ttccaaagta tctagcaata atttataagt
     2101 tagctccatt ccctctttta gcgccttgct atcggtatct gccgctgcta atccatgttc
     2161 caaactatca accactgcca acagctcttt tgcaagcctc tctacagcat atttatgtgc
     2221 attttcgaca tctatcgtgg cgcgtctacg gatattgtct gcatctgcac gtgtgcgcaa
     2281 aaacaaatcc caattctcgg ccgtttttcg cctggcttcc tctagcaaat ctgctaaatt
     2341 ctcggggttt tctgggatca ctggattctc cgatgtctcg gtcgtttggg ctgtttcgtc
     2401 attgttgctc acttgcctct ctcctctttt acggtgggac actgcaggag ccggcctacg
     2461 ccgactcttc acccgcccta caaaattaac agatggggtc tattggtgta aattcaaggg
     2521 aaggcaaaag cacttctgaa ccccattgtt tcagatccgc tagacgatta cactataccc
     2581 tatctagggg cggatcactg tgtccgcctt ataatttacc aaggtagcca aatatgcaca
     2641 agcaaac
//
LOCUS       DAORVT010000161         2630 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_37249_length_2630_cov_3.346796, whole genome shotgun sequence.
ACCESSION   DAORVT010000161 DAORVT010000000
VERSION     DAORVT010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37249_length_2630_cov_3.346796"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..98
                     /locus_tag="PK389_03960"
     CDS             <1..98
                     /locus_tag="PK389_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uL14 family ribosomal protein"
                     /protein_id="HQY22888.1"
                     /translation="QPIGTRIFGPVTRELRGERFMKIISLALEVL"
     gene            109..444
                     /gene="rplX"
                     /locus_tag="PK389_03965"
     CDS             109..444
                     /gene="rplX"
                     /locus_tag="PK389_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011399909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HQY22889.1"
                     /translation="MNKIKKGDAVVIVAGRDRGRQGTVLSVLKSKTRYADAFRLLVEG
                     INLIKKHTKPQQEKAGGIIEKEAPIAISNVAIFNAATGKADRVGFKILENGRKVRVYK
                     STGEVVDHA"
     gene            437..985
                     /gene="rplE"
                     /locus_tag="PK389_03970"
     CDS             437..985
                     /gene="rplE"
                     /locus_tag="PK389_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010324092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HQY22890.1"
                     /translation="MRNTPKLKDFYSKTIVAQLMKDLGCANVMEVPRITKITLNMGVG
                     EAVNDKKVVDNAAADMAKIAGQKPVITKARKSIAGFKVRELWPVGCKVTLRRDRMYEF
                     LERLLCIAIPRIRDFRGLNRRSFDGRGSYSMGIQEQIVFPEIQYDKIDALRGLDITIT
                     TTAKTNEAGLALLLAFQFPFKE"
     gene            993..1298
                     /gene="rpsN"
                     /locus_tag="PK389_03975"
     CDS             993..1298
                     /gene="rpsN"
                     /locus_tag="PK389_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017941084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HQY22891.1"
                     /translation="MARLCKREKDKRQRKLVNKYQAVREELRSRIRDKDLSFEEKLIA
                     QAKMQSLPRDSNRVRLRNRCLVTGRSRGVYRKFGLGRNKLREYAMNGLIPGLRKASW"
     gene            1309..1701
                     /gene="rpsH"
                     /locus_tag="PK389_03980"
     CDS             1309..1701
                     /gene="rpsH"
                     /locus_tag="PK389_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HQY22892.1"
                     /translation="MSLQDPIADMLTQIRNGQMAKKESVTFPASKLKIAILRILEEEG
                     YILSFKKIGDVKPTIMVHLKYFNSKPVIEKIRRVSRPGLRIYKPKDELPKIMGGLGIS
                     ILSTSEGVMTDKKARQCGQGGEVMCAVS"
     gene            1711..2235
                     /gene="rplF"
                     /locus_tag="PK389_03985"
     CDS             1711..2235
                     /gene="rplF"
                     /locus_tag="PK389_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HQY22893.1"
                     /translation="MSRIAKKPLTVPKGVEFNQIGREVHIKGEKGAFKLSLDSAVSIV
                     VEGDLISVGPTAAVHPMVGTTHKLIMNMLQGAHKEFERKLQLVGVGYRAKIEGEILEL
                     SLGFSNPVRFPIPAGISIKVPTNTEIIVSGVDKQVVGETAAKIRKFRPPECYKGKGVR
                     YFGEVVKIKETKKK"
     gene            2245..2613
                     /gene="rplR"
                     /locus_tag="PK389_03990"
     CDS             2245..2613
                     /gene="rplR"
                     /locus_tag="PK389_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HQY22894.1"
                     /translation="MRTTSSHKQRTRARRVNRSRGKVFDLRTVYLSVHRTAQHIYAQV
                     FAPRGSAVLASASSLEAELRTQKIGPGKLKMAEAVGKLVAERAKTKGITKIAFDRSGY
                     KYHGRVKALAEAARANGLIC"
BASE COUNT          809 a          427 c          674 g          720 t
ORIGIN      
        1 tacaacccat tggtacgcgt atctttggtc ccgtaacacg cgaattacgg ggtgagcggt
       61 ttatgaagat tatttcgtta gcccttgaag tgttataggt gaatagctat gaataagatt
      121 aaaaaaggcg atgcagtggt gatcgttgct ggccgtgata ggggacgcca gggtactgtt
      181 ttatccgtac taaagtccaa aacgcgatat gcggatgctt ttcgtctttt ggttgaaggc
      241 attaacttaa ttaaaaaaca taccaagcct cagcaagaaa aagcgggtgg tattatagaa
      301 aaggaagcac ccattgcgat ctcgaatgtt gcaatattta atgcagcaac aggcaaggct
      361 gaccgggttg gatttaaaat attagaaaat ggtcgcaaag taagagtata taagtcgacg
      421 ggagaagtgg ttgatcatgc gtaacacccc gaagttgaaa gatttttata gtaaaaccat
      481 cgttgctcag ctaatgaaag atttaggttg tgcgaatgtg atggaagtgc ctcgtattac
      541 caaaataacc ttgaatatgg gtgtcggtga agcggtaaat gataaaaaag tggttgataa
      601 tgcggctgca gatatggcca aaattgccgg acaaaagcct gttatcacca aagcgcggaa
      661 atctatcgcg ggatttaagg tgcgtgaact gtggccagtg ggttgcaagg ttacattgcg
      721 tcgcgatcgt atgtatgaat ttttagagcg cttactgtgt attgcgattc cacgtattcg
      781 tgattttcgg gggctaaatc gacgttcttt tgatggtcgt ggtagttatt ccatgggtat
      841 tcaagaacaa attgtgtttc ccgaaattca gtacgataaa atcgatgcat tgcgtggatt
      901 agatattacc attacaacaa ccgcgaaaac caatgaagca ggtctcgcgt tgttgttagc
      961 atttcagttt ccttttaagg agtagggctc tcatggcaag attgtgtaag agagaaaaag
     1021 ataaacgtca gcgtaagttg gtgaataaat atcaagccgt tagagaagaa ttacgaagca
     1081 gaattcgtga taaagattta agttttgaag agaaattaat tgcgcaagca aaaatgcaga
     1141 gtttgcccag agattctaat cgcgttcgat taaggaaccg ttgtctagta acgggtcgtt
     1201 cacgaggggt ttatcgtaag ttcggtttag gtcgaaataa attgcgggaa tatgcgatga
     1261 acgggcttat tcctggttta cgtaaggcaa gttggtagga gcatagagat gagtttacaa
     1321 gatcccattg ccgatatgtt gacccagatc cgtaatggac aaatggctaa aaaagaaagc
     1381 gttacctttc cggcttctaa attaaagatt gccattctgc ggatattaga agaggaaggt
     1441 tacattctat cgtttaagaa aataggtgat gtaaaaccaa ctattatggt tcatttgaaa
     1501 tattttaatt cgaagcctgt tatagagaag ataagacggg tcagtcgtcc gggcttgcga
     1561 atttataagc caaaagatga attacctaaa ataatgggtg gattaggcat ctcaatttta
     1621 tcgacatccg aaggtgttat gaccgataag aaagcgcgtc aatgtggaca gggcggtgaa
     1681 gtcatgtgtg ctgttagcta ggagtaatca atgtccagaa ttgcgaaaaa gccattaacc
     1741 gttcccaaag gcgttgaatt taatcaaatt ggccgggaag tgcatattaa aggcgagaaa
     1801 ggtgctttta aattatccct cgactcagca gtttcgattg tcgtagaggg agaccttatt
     1861 tctgtggggc cgaccgcagc ggtacatccg atggtcggaa ctacccataa gctaattatg
     1921 aatatgctgc aaggcgccca taaagaattt gaacgaaaat tgcagttagt tggggtggga
     1981 tatcgtgcaa aaattgaagg cgaaatttta gaactgagtt taggattttc gaatcctgtg
     2041 cgattcccaa tacctgcagg tatcagcatt aaggtgccta cgaatacgga aattattgtt
     2101 agcggtgttg ataagcaagt tgtgggtgaa acagctgcta aaattcggaa gttccgtcca
     2161 ccagaatgtt ataaaggcaa gggtgtgcgg tattttggtg aagttgtaaa aatcaaggaa
     2221 actaaaaaga aataggatga tatgatgcgt actactagta gtcataaaca aagaacacgt
     2281 gcacgccgag taaatcggtc tcgaggtaaa gtatttgact tgcgtaccgt ttatttaagc
     2341 gtgcatcgta cggcgcagca tatttatgcg caagtttttg caccgagagg gtcggctgtt
     2401 ttggcttcgg cttctagcct tgaagcagaa ctgcggactc aaaaaatagg tccaggaaag
     2461 ttgaaaatgg cagaagccgt tggaaaattg gtggcagaac gcgcaaagac taagggtatt
     2521 acaaaaatag cctttgatcg ttctggttat aaatatcatg gtcgtgttaa agctttggcg
     2581 gaagcagcgc gagctaatgg cttaatttgt taagggttta aaaacatggc
//
LOCUS       DAORVT010000162         2618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_37561_length_2618_cov_4.758876, whole genome shotgun sequence.
ACCESSION   DAORVT010000162 DAORVT010000000
VERSION     DAORVT010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37561_length_2618_cov_4.758876"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="PK389_03995"
     CDS             complement(<1..641)
                     /locus_tag="PK389_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HQY22895.1"
                     /translation="MRNLHGKALLVDPALTKSTAFTREERKQYGLRGLIPYDVADINK
                     QIARVLGSMRCKSSDIEKYIFLNALLERNQRLFYRMMLDHIEEIMPLVYTPTVGEACK
                     EFAHIFRNPQGFYITPEDRGDIATILGNWPEKDIRVIVVTDGERILGLGDLGANGMGI
                     PIGKVALYVACAGINPAQCMPIMLDVGTNNIALREDPLYLGYPYPRIQGDEYRS"
     gene            873..1298
                     /locus_tag="PK389_04000"
     CDS             873..1298
                     /locus_tag="PK389_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HQY22896.1"
                     /translation="MNQSPFPIPNPNLYTEEEVAQLVHQFYAKARKDPGLGPIFEAHV
                     IDWDAHFVQMTNFWSAQLRGTSRFRGAPMPKHIILPDLTAELFERWLQLFKQTTEELG
                     NPNLQQHANAVATFIAGRLWQGYQMNNYPDKQLEALRTV"
     gene            1534..>2618
                     /locus_tag="PK389_04005"
     CDS             1534..>2618
                     /locus_tag="PK389_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HQY22897.1"
                     /translation="MKSIKNKIIVFSILATLIPSLGLGILSFQQNETMISENVTRELR
                     ALANHASREFDLWVKDQIYIARELSTSKILIEGLSSINRSQSQKNKNIAQQKVLEYYL
                     KSVYKKLETVRELTVVDIDGSIIASNVESPVPVILPKNWPESASIQGDVVVPPHWNTH
                     YATATLSISVPILSVNDFILGALIVTFDLHNIQSSLKDTVKSPPGDVLLLDRDGNVML
                     ASGDFLALDITQPLSLDPVILKRLQDNPGEYEIFHGISQEKVIGLAYTSKKPLLTIIA
                     NRNYQSVYATWEEQRNLFISLIGIILLLVTAIALQMSRTIVIPLRKLINATEKIVKGD
                     LDVELTIAQRDELGQLTRMFNQMTDKLR"
BASE COUNT          806 a          567 c          477 g          768 t
ORIGIN      
        1 gatcgatatt catccccttg tatacgggga tatgggtagc ctagatacaa cggatcttca
       61 cgcaaggcga tattattcgt gcccacatct agcatgatag gcatgcattg cgctggatta
      121 ataccagcac aggctacgta tagagccact ttaccaatag gaattcccat cccattggca
      181 ccaagatccc ccaaacccag aatgcgctct ccatcggtca ctacaatgac tcgaatatct
      241 ttttcgggcc aatttcccag aatggttgca atatcgcctc gatcctcagg cgtgatataa
      301 aatccctgtg gattcctgaa aatatgtgca aactctttgc acgcctcgcc tacggtcgga
      361 gtataaacca aaggcataat ttcttcgata tgatcaagca tcattcgata aaaaagccgc
      421 tgattacgct ctagcagcgc atttaaaaaa atatattttt ctatgtcgct gcttttgcaa
      481 cgcatgctac ccagaacgcg tgcgatttgt ttattgatat ccgctacatc atacggaatc
      541 aatccacgca aaccatattg cttgcgctcc tcgcgcgtaa acgcggtcga ttttgtcaat
      601 gcagggtcaa ccaacagagc ctttccatgc aaatttctca tgttcattca catctccaag
      661 ctaaattctc tttctaatga aagatataaa caatttcact caattcacaa ttggacattc
      721 cgaacagtga aagataaact tatctatccg ataaaaatgt gttttataga tttattttta
      781 catgcacatt aaggaatcag tttagtcttt ctaatctaca attgcagatc ttttaacgaa
      841 accttctttt caagaacgac cctaattaaa ctatgaatca atcacccttt ccaattccta
      901 atccaaattt atacactgaa gaagaagtcg cccagcttgt tcatcaattt tatgcaaaag
      961 ccaggaaaga tcctggattg ggtcctatct ttgaagcaca tgtcattgat tgggatgctc
     1021 attttgtgca aatgactaac ttctggtcag ctcaattgcg cggaaccagc cggtttcgcg
     1081 gagctccaat gccaaaacat attatcctac ctgatcttac cgcagaatta ttcgaacgtt
     1141 ggttgcagct ctttaaacaa accaccgaag aattaggcaa ccctaattta caacaacatg
     1201 ctaacgcagt cgctaccttt atcgctggca gattgtggca gggttatcag atgaataatt
     1261 atccagataa gcaacttgag gcgttaagaa ctgtctaatt gattgaatta gatagtaatt
     1321 tatagctctt ttgttttaaa gctccctgtc acggcgacag ggggcttata taatcgcacg
     1381 gtcacaaatt tgctgacttt caatcggtat tctcttccga agttggaaaa atagctctgt
     1441 aaatattaaa aagagataaa aaagtatcct tattcgattt tattattaca ttgacagcac
     1501 tgtaatattc tttatgtcat aaggatattt gcattgaaaa gtataaaaaa caaaattatt
     1561 gttttttcca ttcttgctac tctgattcca tcattaggat taggtatcct atcgtttcag
     1621 caaaatgaaa cgatgatcag tgaaaatgtg acacgcgaat taagagcact ggcaaatcat
     1681 gctagtcggg aatttgatct ttgggttaaa gatcaaattt atatcgcacg agaactgtca
     1741 acttcaaaaa ttcttattga aggattatct tcaattaatc ggagtcaatc acagaaaaac
     1801 aaaaatattg cgcagcaaaa agtgcttgag tactacttga agtctgtcta taaaaaatta
     1861 gaaaccgtac gagaacttac tgtagttgat atagacggca gtattattgc cagcaatgtt
     1921 gagtcccctg ttccggtcat tctcccgaaa aattggccag aaagcgcatc catacaaggt
     1981 gatgtagtcg ttccaccaca ttggaatacc cattacgcaa ccgcaacgct cagtatttca
     2041 gttccgatat tatcggttaa cgattttatc ctgggcgcgc tgatcgtcac attcgatttg
     2101 cacaatattc aatccagttt aaaagacacc gttaaatctc cacccggcga cgttctattg
     2161 ctcgatagag atggtaatgt gatgttagca agtggcgatt ttttagccct cgatatcact
     2221 caaccgctat cgcttgatcc agtgatattg aaacgattac aggataatcc gggtgaatat
     2281 gaaatctttc atgggatatc acaagaaaaa gtcatcggtt tagcctatac gtcgaaaaaa
     2341 ccgctactca caatcatcgc taataggaac tatcagtctg tatatgccac ttgggaggag
     2401 caacgtaact tattcatcag tttgattggc atcattctcc tattggtcac cgcgatcgct
     2461 ttacaaatga gccgcaccat agtcattcct ttgcgtaaat tgatcaatgc aaccgagaaa
     2521 attgtaaagg gagatctcga tgttgaacta accattgcac agcgcgatga attgggtcaa
     2581 ttgacacgca tgtttaatca aatgactgac aagttgcg
//
LOCUS       DAORVT010000163         2618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_37566_length_2618_cov_3.481077, whole genome shotgun sequence.
ACCESSION   DAORVT010000163 DAORVT010000000
VERSION     DAORVT010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37566_length_2618_cov_3.481077"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /gene="cysS"
                     /locus_tag="PK389_04010"
     CDS             <1..1177
                     /gene="cysS"
                     /locus_tag="PK389_04010"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HQY22898.1"
                     /translation="RAQEKNISFSALTAEFIQAMNTDFQHLNILPPTAEPRATNYIQQ
                     MIDMIEQLVDKGYAYVGETGDVYYDIQKFESYGCLSHRDLEDLKAGSRVEINDAKQHP
                     FDFVLWKMAKPKEPSWESPWGLGRPGWHIECSAMSLVQCGETFDIHGGGPDLKFPHHE
                     NERAQSEAANGKKFVNYWMHVGFVQQDKEKMSKSLGNFLTIRDFLKHYHPEVLRYFSI
                     MSHYRSPIDYATDNIDAAGRGLSRLYTALRGISLENPVEKNTLTIQSLFEQKFEDAMN
                     DDFNTPEAIAVLFEIAHEINRQRETEPEKAIVLAIRLKKLGNILGLLYSEPEVFLQNA
                     SGKAIDLAFSEIEQLIAARDQARKSKQWIESDRIRDILLEKGIVLEDTAKGTTWHAR"
     gene            complement(1182..2003)
                     /locus_tag="PK389_04015"
     CDS             complement(1182..2003)
                     /locus_tag="PK389_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HQY22899.1"
                     /translation="MLGFGLGLRTDHYSSILDTRPQIDWFEILSENYMISGGKPLYFL
                     DQIREHYPMAMHGVSLSIGNTDSLNWDYLKQLKDLAVRVKPAWISDHLCWTGINKQNI
                     HDLLPLPYTTETIEHVVLRVKAVQDFLGQQILLENVSSYITFKNSELSEWDFLSAIAI
                     EADCKILLDINNIYVNSFNHGFDPYQYLEGLPCDRIQQFHLAGHTHNVDHIIDTHNAP
                     IVDPVWQLYAAAIKRFGRTATLIERDDNIPPLNELLTELKKAKAIAEQNYGAVQK"
     gene            complement(2156..2365)
                     /locus_tag="PK389_04020"
     CDS             complement(2156..2365)
                     /locus_tag="PK389_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22900.1"
                     /translation="MKQLILAAVAATLVFASAAQAAAPATTTTTTTTVVSPMDADACK
                     KAMEACGADQACKDALIKDHSCKAE"
BASE COUNT          790 a          452 c          589 g          787 t
ORIGIN      
        1 cagagcgcaa gaaaaaaata tttcttttag tgcattaaca gcagaattta ttcaggctat
       61 gaatacggat tttcagcatt taaatatcct gcctccgaca gcggaacctc gagcaacgaa
      121 ttatattcag cagatgattg atatgatcga gcaactggtt gataagggat acgcctatgt
      181 gggtgaaaca ggcgatgttt attacgatat ccaaaaattt gagagttatg gttgcttgtc
      241 gcatcgggac ttagaagatt taaaagcggg ctcgcgtgta gaaattaatg atgcaaaaca
      301 gcatcctttt gattttgtgt tatggaaaat ggctaaacct aaggaaccga gttgggaatc
      361 tccttggggt ttaggacgtc caggttggca tatagaatgt tctgccatgt ctttagtgca
      421 gtgtggcgag acgtttgata tccatggtgg gggtccggat ttgaaatttc cacatcatga
      481 aaatgagcgt gcgcagtcgg aagcggcaaa tggcaagaaa ttcgtgaatt attggatgca
      541 tgtcggattt gtacagcaag ataaagaaaa aatgtctaaa tcgctgggta attttttaac
      601 gatcagagat tttttaaagc attatcatcc agaagtattg cggtatttta gtattatgag
      661 tcattatcgt agcccaatcg attatgcaac agataatatt gatgctgctg gtagagggct
      721 ttcgcgactc tatacggctt tgcgcggtat ctcgttagaa aatcctgttg agaaaaatac
      781 actgacaata caatcactct tcgagcaaaa atttgaagat gcgatgaacg atgactttaa
      841 tacccccgaa gccattgccg ttttatttga aatagcccat gaaattaatc ggcaaaggga
      901 gactgagcct gaaaaagcga tagtgcttgc gatacgctta aaaaagcttg gcaacatctt
      961 agggttgttg tatagcgaac ccgaagtatt tctgcaaaat gccagtggta aagcaattga
     1021 tttagcgttt agcgaaattg aacagttgat tgcagcgcga gatcaggcta gaaaatctaa
     1081 acagtggata gaatccgata ggatccgaga tatattatta gaaaagggta ttgtgctaga
     1141 ggatactgca aagggcacaa catggcatgc gcgttagata attatttttg gacagcgccg
     1201 tagttttgtt ctgcaatcgc ttttgctttt ttgagttctg ttaataattc atttaaaggc
     1261 ggaatattat catctcgttc tatcagggtt gcagtccggc caaagcgttt aattgcagca
     1321 gcgtacagct gccaaaccgg atcaacaatg ggagcattat gtgtgtctat gatgtgatca
     1381 acgttatgag tatgccctgc aaggtgaaat tgttgaatgc gatcgcaggg tagcccttca
     1441 aggtattgat agggatcaaa gccatggtta aaactattga cgtaaatatt attaatatct
     1501 aataaaattt tacaatccgc ttctatagca atagcactga gaaaatccca ttcggagagt
     1561 tcagaatttt taaaagtaat atagctggat acattttcta gcaaaatttg ttgacctaaa
     1621 aaatcttgta cagctttaac acgtaaaacc acatgttcaa ttgtttcagt tgtgtagggt
     1681 aggggtaata aatcatgaat attttgctta ttgatgcctg tccaacagag gtgatctgat
     1741 atccaagcag gctttacacg aactgctaaa tcctttaatt gctttaaata atcccaattg
     1801 agcgaatcgg tgttgccgat agataatgat accccgtgca tggccatggg gtagtgttcg
     1861 cggatctgat caaggaaata gagaggtttt cctccggaga tcatatagtt ttcggataaa
     1921 atttcaaacc aatcaatttg aggacgggtg tctagtatgc tgctatagtg atccgttcgt
     1981 aacccaagcc cgaatcctaa cataaattct ccatgttcag gctcgaccat ttttgtaaga
     2041 atagaataaa aacggagata ctacaacgaa gttttttcag aaaggagata aaaaaagggc
     2101 aggtttttag acctgccctt ttttattgca aaactagcga tgtgctagat ctgacttatt
     2161 cagctttgca gctatgatct ttgatcagtg catctttgca agcttgatct gcaccacaag
     2221 cttccatcgc ttttttgcag gcgtctgcat ccattgggct aacgacagtc gtcgttgtgg
     2281 tagtagttgt cgcaggagct gctgcttgtg cagcagaagc aaatacaagt gttgcagcaa
     2341 ctgcagctaa aattaattgt ttcattggta actctccaaa agaaaatgac gtcattaaat
     2401 tacggagtta cccaaacaat ctggtaaccc cgtggttatt gtcagaactt gaactggcta
     2461 accgtagttt ccaataaaaa tttctgacgc ttgcatagtt tagcatacaa ttttgaggtg
     2521 tctagttttt ttttactatc gttaaagact gtttttttag gtatgtgaga tgtgaatcgc
     2581 tggcgaagcg ggtagcttaa tgtcatattt gttaaaag
//
LOCUS       DAORVT010000164         2608 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_37831_length_2608_cov_2.860556, whole genome shotgun sequence.
ACCESSION   DAORVT010000164 DAORVT010000000
VERSION     DAORVT010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37831_length_2608_cov_2.860556"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..895)
                     /locus_tag="PK389_04025"
     CDS             complement(110..895)
                     /locus_tag="PK389_04025"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22901.1"
                     /translation="MKVTNFNQWPTTGTLLLVGENGSERQNPETLALQYARKVLSENS
                     DRSGILFDAKTHTDFQFVTPEPKSQWIKIDQIRELIEWASGKPQIASKQVAIISPAHA
                     LNIHAANALLKTLEESPPDALFILATDRPSFIPATIRSRCYWIRCREKELESTHTPIT
                     PDAIALKAQISEDLNALKTKTTDPVSVGIQWIKHDPKQILYWLLVILQTQIKNAMLQS
                     TSSHRTLWRFLDKALDAKRELEAPNQPNTQLLMESLLIEYVNL"
     gene            complement(892..1533)
                     /gene="tmk"
                     /locus_tag="PK389_04030"
     CDS             complement(892..1533)
                     /gene="tmk"
                     /locus_tag="PK389_04030"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007160593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HQY22902.1"
                     /translation="MFITFEGIEGVGKTTHLKWIAQQLQRAGISVLTTREPGGTPIGE
                     EIRDILLAHRHERVAPLTELLLMFAARAQHVDTVIRPALELGHWVLCDRFVDATYAYQ
                     GGGRGVETDLIAKLEKLVLGNFKPDLTLLFDAPIALGLSRIKERGGVQDRFEQEKLDF
                     FERVRLAYKTRAEQDPLRHKIIDASKTIDEVQAELLAMITTFYNFSHSGRALE"
     gene            complement(1533..>2608)
                     /gene="mltG"
                     /locus_tag="PK389_04035"
     CDS             complement(1533..>2608)
                     /gene="mltG"
                     /locus_tag="PK389_04035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HQY22903.1"
                     /translation="KKLFKFLVFSMSVLSIFAATLSYQYQDFLHRPLTIDPHSPEGIV
                     FVVPMGTPVYKIVNNLVEAGVLQSSNAFLLAVFLHDSWHTLKAGEYLIKPGSTPVDLI
                     NQLRDGKVIQYALTIVPGWTFERLMLEIAGSSKIQHALTGLSPAEIMTKLGHATEHPE
                     GRFFPETYYYPLGTTDIAILQRSYRDLQAKLSLLWNTKKMDSVLKSAYDALILASIIE
                     KESSVVDEYRDIAGVYTRRLAKNMPLQADPTVIYGAGKTGEGTRSNSNITKEMLAHQN
                     PYNTYLKAGLPPTPIALPSAKALEAALDPKAGETLYFVAKPDGKGHIFTKNLTDHNSA
                     VAEYRKGLVNTTLNSTNNTKSTP"
BASE COUNT          694 a          535 c          544 g          835 t
ORIGIN      
        1 tgggtatttc tgccattact tttgctctcc atttttttat aatatgctta tctttaatga
       61 ttatagttat aatataagct ttttaaagcc tggttaacct atagttaatc taaagattga
      121 cgtattcaat taatagcgat tccattagca attgtgtatt tggctgatta ggcgcctcta
      181 actcgcgttt agcatccaat gccttatcta aaaatcgcca taatgttcta tgagaggatg
      241 ttgattgcaa catagcgttt ttaatttgcg tctgcaaaat aaccaatagc caatataaaa
      301 tttgttttgg atcgtgttta atccactgga tcccaacaga gaccggatct gttgtttttg
      361 ttttaagtgc gttcaagtcc tcgctaatct gtgcttttaa tgcgatggca tctggcgtaa
      421 taggcgtatg ggtgctttcc agctcttttt cgcggcaccg tatccagtaa caacgactac
      481 gtatggtcgc ggggataaat gagggtctat cggtggctaa tataaataat gcgtcgggag
      541 gggattcttc cagggttttt agcaatgcat tggcggcatg gatattcaac gcatgcgctg
      601 gcgatataat cgcaacttgt ttgctcgcaa tctggggttt tccgcttgcc cattctatta
      661 gttcacgaat ttgatcgatt ttaatccact gcgattttgg ttcgggcgtt acgaattgaa
      721 agtcggtatg ggttttagcg tcaaagagga tcccgctgcg atcggaattc tcagataaaa
      781 cttttcgggc atattgcaat gcaagtgtct ctggattttg cctttcagat ccattttctc
      841 cgaccaatag tagcgtgcct gttgttggcc attggttaaa atttgtgacc ttcactctag
      901 cgctctccca gagtgagaaa agttataaaa agtggtaatc attgctaaca gctcagcttg
      961 tacttcatcg attgtttttg aggcatcgat aattttatgt cgcaaaggat cttgttcggc
     1021 ccttgtttta taggctaaac gcacgcgttc gaaaaaatcc aatttttctt gctcaaatct
     1081 atcttgaaca ccaccacgct ctttaatacg actaagtcct agcgctatgg gggcatcaaa
     1141 gagtaaggtt aagtctggct taaaattccc taagactaat ttttcaagct ttgcaattaa
     1201 atcggtttct acccctcgcc cgcccccttg atacgcatac gttgcatcga caaaccgatc
     1261 acaaagcacc cagtggccga gttctagtgc agggcgaatg acagtatcaa catgttgcgc
     1321 tcttgccgca aacatcagta ataattccgt taaaggcgct acgcgttcgt gtcgatgtgc
     1381 taataaaata tcacgaattt cttcgcctat gggcgttcca ccgggttctc gggtagtcag
     1441 cactgaaatg cctgcacgct gtaattgttg ggctatccat tttaaatgcg tggtttttcc
     1501 aacgccttct atgccctcaa atgtaataaa cattaaggtg tactctttgt attgtttgtt
     1561 gaatttagtg ttgtgttgac aagccccttt cgatattccg ccacagcaga attatgatcg
     1621 gtcaaatttt tcgtaaaaat atgtccttta ccatcgggtt ttgcaacaaa ataaagtgtt
     1681 tcacctgctt tgggatctaa agctgcttcg agcgcttttg cactgggtag cgcgataggg
     1741 gttggcggta atccggcttt taggtaagta ttataagggt tttgatgagc taacatctct
     1801 ttggtgatat tgctattaga tcttgtgccc tcgcccgttt ttcccgcgcc ataaataaca
     1861 gtcggatcag cctgcaaggg catatttttt gctaagcgtc tggtataaac accggcaatg
     1921 tcgcgatact catcaaccac actcgattct ttttctatga tagaggctaa aattaatgca
     1981 tcgtaggcag attttaatac tgaatccatt ttttttgtat tccacagtaa cgataatttt
     2041 gcctgtaaat cccgataaga tcgctgcaat attgcaatat ctgtcgtacc cagaggataa
     2101 taataagtct ccggaaaaaa tcgaccctct ggatgttctg ttgcatgtcc caattttgtc
     2161 ataatctctg caggacttaa tcctgttaaa gcatgctgaa tttttgaaga gcctgcaatt
     2221 tctaacatta agcgttcgaa ggtccaaccg ggaactatcg ttaaagcata ttgaatcact
     2281 ttgccatcac gcaattgatt aatcaaatca acgggggtac tgccgggttt aatgagatat
     2341 tcgcctgctt ttaatgtgtg ccaactgtcg tgcaaaaaca ccgctaacaa aaacgcattt
     2401 gatgattgta aaacgccggc ttcaactaaa ttgtttacga ttttataaac aggggtgccc
     2461 atgggcacaa caaatacgat gccttccggc gaatgagggt cgatagttaa aggtctatgt
     2521 aaaaaatcct gatattgata acttaaagtc gcagcgaaga tgctgaggac gctcatagaa
     2581 aagacgagaa atttaaaaag ttttttca
//
LOCUS       DAORVT010000165         2600 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_38056_length_2600_cov_3.019253, whole genome shotgun sequence.
ACCESSION   DAORVT010000165 DAORVT010000000
VERSION     DAORVT010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38056_length_2600_cov_3.019253"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="PK389_04040"
     CDS             <1..992
                     /locus_tag="PK389_04040"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY22904.1"
                     /translation="ISVRFQAKSAFDLGCNTQLRMQERKILITGSKGLIGHALKSQLK
                     TCGFNVIEVDLRYPKNHHKHGDIRDLKQLEKLSDDCHGIVHLAGVSRVISGEKNPELC
                     WDTNVNGTQAVLEAALKSTRRPWVIYASSREVYGQQTVLPVDERAPLLPVNIYAKSKI
                     AAESAVLEYRAKGLQTVILRFSNVYGSLNDYIDRVIPAFCRAAALGETIRIDGGHNVF
                     DFTYIEDVIDGIIKVISRLEQGNCDLPAIHFTTGKGTTLLEAATIAKKLSNFNIHFQE
                     MPSRTFDVATFYGDPRLALDLLGWKATVMIEEGIRRLVRLFESQSIYASKSCV"
     gene            999..2297
                     /locus_tag="PK389_04045"
     CDS             999..2297
                     /locus_tag="PK389_04045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY22905.1"
                     /translation="MKILKVIHGYPPLYNAGSEVYSQMLCHGLSKNHEVHVFTREENP
                     FVKDFSLRETFDVLMPNIKIHIVNIPLEKYRYQYRHAEIDKIFADLLLKIKPNIVHIG
                     HLNHLSTSMVWEAEKQKIPLIYTLHDYWLMCPRGQFIQRNPPQNDELWALCESQENRK
                     CAVNCYSGYFSGAEGEINTDLKYWTDWVDRRMTHIRDILKNISCFIAPSKYLYQRYVQ
                     EFGLPMEKVLYLDYGFDLKRLQNRKRLLGEPFTFGYIGTHIPAKGIQHLISAFGLLKG
                     RCQLRIWGRPRSQNTEALRSLVASLPVEIQNAVYWLSEYRNEHIITDVFNLTDCIVVP
                     SIWVENSPLVIHEALQAEVAVITANVGGMAEYIQHEKNGLLFEHRNVNSLAMQMQRLV
                     NNPDEARRLGTIGYLQSDDANIPNLDAHVDAIEDIYRKFA"
     gene            2309..>2600
                     /locus_tag="PK389_04050"
     CDS             2309..>2600
                     /locus_tag="PK389_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002615800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQY22906.1"
                     /translation="MEKRPGPWRITFDTNPDDCNLSCIMCEDHSPYSTTRVDRIAAGI
                     PRRRMSIDLIKQILAELQGTPLQEIIPSTMGEPLLYKDFEEIIKLCHQYNIKL"
BASE COUNT          808 a          438 c          561 g          793 t
ORIGIN      
        1 aaatcagtgt tcgatttcaa gcgaaatcgg cgttcgattt ggggtgtaat acacaattac
       61 gcatgcagga aagaaagata ctaattacag gttccaaagg attgattggt catgcgctaa
      121 aaagtcagtt gaaaacctgc ggctttaatg ttatagaagt tgatctgcgt tacccaaaaa
      181 atcatcacaa acatggcgat attcgggatt taaaacaatt agaaaaatta tctgatgatt
      241 gtcatggtat tgttcattta gcaggggtgt cccgcgtcat ctctggcgaa aaaaatcccg
      301 agctttgctg ggatacgaat gttaacggta cacaggcagt gctagaagca gctctaaaat
      361 ctactcgacg accttgggtt atttatgcga gcagcaggga agtctatggg caacaaacgg
      421 tattgccggt tgatgagcgt gcacccttat tacctgtcaa tatttatgca aagtctaaaa
      481 tcgctgcaga aagtgcagta ttagaatata gagctaaagg cttgcaaacg gttattcttc
      541 gtttttctaa cgtatatggt agccttaatg attatattga tcgtgtgatc ccagcctttt
      601 gcagagcagc ggcattgggt gaaacgattc gtattgatgg aggccataat gtatttgatt
      661 ttacctatat tgaggatgtg attgatggaa tcataaaagt gataagtcga ttagaacaag
      721 gaaattgtga tttgcctgcc attcatttca caacgggtaa aggtacgact ttgctggaag
      781 cagccactat tgcaaaaaaa ttaagtaact ttaatatcca ttttcaagaa atgccttcac
      841 gtacatttga tgtggcaacc ttttacggcg atccacgttt ggcattagat ctcttgggct
      901 ggaaagcaac agtgatgatt gaagaaggta tcaggcggtt agttagattg tttgaatcac
      961 aatctattta cgctagcaaa agttgtgttt gatatagaat gaaaatattg aaagtcattc
     1021 atggttatcc tccgctttac aacgcgggat cagaagttta tagtcaaatg ctctgtcatg
     1081 gcttatctaa aaatcatgaa gtgcacgttt ttacacgtga agaaaaccca tttgtgaagg
     1141 atttttcgct tagagaaacg tttgatgttc ttatgcctaa tatcaagatt catattgtta
     1201 atattccgct tgaaaaatat cgctaccagt atcgacatgc tgaaatagac aaaatttttg
     1261 ccgacttatt gctaaaaatt aaaccgaata ttgttcatat tggccatctt aaccatctgt
     1321 cgacttctat ggtatgggaa gcagaaaaac aaaaaatacc gttaatatat accttgcacg
     1381 attattggtt gatgtgccct agaggtcaat ttattcagcg aaatccgcca cagaatgatg
     1441 aattatgggc attatgtgag agtcaagaga atagaaagtg tgcagtaaat tgttattcag
     1501 gctacttttc gggggctgag ggagaaatca ataccgatct aaagtattgg acggattggg
     1561 tggatcgtcg aatgacgcat attcgtgaca ttctaaaaaa tataagctgc tttattgcgc
     1621 cttcaaaata tctgtatcaa cgttacgtgc aagagtttgg attgcccatg gaaaaagttc
     1681 tttacttaga ctatgggttt gatttaaaac ggttacaaaa tcgaaaacgt ctcttaggag
     1741 agccttttac ttttggttat attggtaccc acataccggc taaagggatc cagcatttaa
     1801 tttcagcttt tggtttatta aaaggccgtt gtcaactacg tatttggggt cgaccgcgct
     1861 cgcagaatac agaggcgtta cgttctcttg tagcaagctt gcctgtagaa attcaaaacg
     1921 ccgtttattg gttgtctgaa tatcgtaatg aacatattat aacggatgtt tttaatttaa
     1981 cagattgtat tgttgttccc tctatttggg tagaaaactc gcctttagtt atccatgagg
     2041 ccttacaagc cgaagtcgcc gtgataactg caaatgtagg tggcatggca gaatatattc
     2101 aacatgaaaa aaatggttta ttgtttgaac accgcaatgt taatagtctt gcgatgcaaa
     2161 tgcagcggtt ggttaataat cccgatgaag cacgtagatt aggaactatc ggttatctgc
     2221 aatcggatga tgctaatatt ccaaatttag atgcacatgt tgatgctata gaggatattt
     2281 atcgtaagtt tgcataagga gaatgtctat ggaaaagcgg cctgggccat ggcgtatcac
     2341 atttgatacc aatcccgatg attgtaattt aagttgtatt atgtgtgaag atcattctcc
     2401 ttatagtacg acgagagtcg atcgcatagc agctgggata cccagacgcc ggatgagtat
     2461 tgacttaatc aagcagattt tagcagaatt acaaggaaca ccactacaag aaattatacc
     2521 ttccacaatg ggtgagccgt tgttatacaa agattttgaa gaaattatta aattatgtca
     2581 ccaatataat atcaaattaa
//
LOCUS       DAORVT010000166         2570 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_38827_length_2570_cov_3.187276, whole genome shotgun sequence.
ACCESSION   DAORVT010000166 DAORVT010000000
VERSION     DAORVT010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38827_length_2570_cov_3.187276"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="PK389_04055"
     CDS             complement(<1..976)
                     /locus_tag="PK389_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22907.1"
                     /translation="MLRRDSKESRVTQDAVAKAKAENDLKLLLESKSFINKSKGIDQS
                     KEKAAELFEFLVNHFDNIKGSLIHTDAEEIDHCYLVEIVKEMSNNISKENEKYREEIS
                     AKREISKNEKALSENEALLEKLVQIDNALAVPLKILRDQEEAKDKDRLRASFSEAIRG
                     DIFSRSTLGMQSEMCEKVTDSAMHDREYFNGTYNALEAKVWLERDKRKEKFNRILNSL
                     VKWGQKDGVKTAIQIGGVVAIGVTSGGIGLGAALLTKVGAQALSKGHEAFLEKYYKVP
                     LEYKEHLETFEANAKLLKANIDSLVRLKIKRDAATGEAKKLLDLQIRVV"
     gene            complement(1502..2071)
                     /locus_tag="PK389_04060"
     CDS             complement(1502..2071)
                     /locus_tag="PK389_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22908.1"
                     /translation="MKRILLFITLPVIFGLINGCAPNVSPNTYEASESGVASKVVSGL
                     IVGKRAVKVDAKSGLGGLAGAGVGAAGGSAIGGSPQANIAGAIGGAIVGGLVGNAVDK
                     TANSHKAFEYIINLKNGSTISIAQVQEMEFQVGQHVLVIYGAMTRIVPDNTIQPVDSA
                     KPAAKSNTTVKKKKTVTQQVEKTVSYNEI"
     gene            complement(2150..>2570)
                     /gene="fur"
                     /locus_tag="PK389_04065"
     CDS             complement(2150..>2570)
                     /gene="fur"
                     /locus_tag="PK389_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HQY22909.1"
                     /translation="KKAGLKVTAPRVKILHILETSLQSADIRHLSAEDVYKKLLEMGD
                     DVGLATVYRVLTQFESAGLVIRHHFEGGHSVFELDDGKHHDHLVCIKCRQVVEFIDPV
                     IEQRQEAIAKEKQFRMTDHALYIYGVCIRCEDQEGYV"
BASE COUNT          704 a          592 c          401 g          873 t
ORIGIN      
        1 ctaccactcg tatttgtaaa tccaatagtt tttttgcttc tcctgtcgca gcatcccttt
       61 taatttttaa tcttacaagt gaatctatat ttgctttaag caattttgca tttgcttcga
      121 atgtctctaa atgttcttta tattctagtg gtaccttata atatttctct aaaaatgcct
      181 catggccttt gctaagagct tgcgcgccca ctttagttaa caatgctgca ccaagaccta
      241 ttccccctga agtcacacca atggcaacaa caccgccaat ctgtatcgct gttttaaccc
      301 cgtctttctg accccacttc accaaactgt tcaatattct attaaacttt tccttccttt
      361 tatctctttc caaccatacc tttgcttcaa gcgcattgta cgttccgtta aaatattctc
      421 gatcatgcat tgctgagtct gttacctttt cacacatctc actctgcatt cctaatgttg
      481 atctactaaa aatatctccc ctgatcgctt cgctgaatga tgctctcaat cgatctttgt
      541 cttttgcttc ttcttgatct cttaaaattt ttagaggtac tgctagagca ttgtctattt
      601 gaacgagttt ttctagtaat gcttcattct ctgatagtgc cttttcgttt ttcgatattt
      661 ctctcttagc actaatttct tctctatatt tttcattttc tttcgatata ttgttgctca
      721 tctcctttac tatttcaact aaataacagt gatctatttc ctcagcatcc gtatggataa
      781 gcgacccttt tatattgtca aaatgattta ctaaaaattc aaaaagttca gctgcttttt
      841 ctttactttg atcgattcct ttacttttat tgataaatga tttactttcg agaagtaatt
      901 ttaaatcgtt ttctgctttc gcttttgcaa cagcgtcctg tgtaacccta ctttctttcg
      961 aatctcttcg aagcataata aaccacctat acaaaagcaa taattgaaaa aagccaatat
     1021 atctatatga ctataggtat cggaaaaaat tcaatattta taaagtaggc ctcaaatgag
     1081 gtatatttat gcaggtcaat aatgtgtgca ggtacacagc ctggcgccat gggatttgca
     1141 ccgctgttaa gcaacaattc tactaattct ggattgtttc gtcttatcgc atgctgcagc
     1201 ggtgtagtac catctgtgtc tgaacagtaa tttcaatgaa ggtacacgaa aattttcaca
     1261 gaaaaaggcg gcagagagcg acgaatgagc gttttttagc gccccgagaa aaaactatcg
     1321 agcatttctt gttttgcgtc attaaccgca acattaagcg cttttgcaaa gttcgggtaa
     1381 tgcgggcttt gatcccaagc accttctact ggctgaataa tttcggtttc gtgatgccac
     1441 cagacttttt tattttaaag atatggccaa cagcaaaaat attttacggg taacaaccgt
     1501 actatatttc attgtaactg acggtttttt caacttgttg cgtgactgtc ttcttttttt
     1561 tgacggttgt atttgatttt gcagcaggct tagctgagtc aacaggctga atcgtattat
     1621 cgggtacaat gcgtgtcatc gcaccataaa tcactaatac atgctgtccc acttgaaatt
     1681 ccatttcttg aacctgtgca atcgaaatcg ttgagccatt tttcaaatta ataatgtatt
     1741 caaatgcctt atggctattc gctgttttat cgactgcatt accaacgagg ccaccaacaa
     1801 tagcaccacc aatagcgcct gctatattcg cttgtgggct tccgccgatg gcagatccac
     1861 ctgcagcacc aacaccagcg cctgctaatc caccaagccc actttttgca tcaactttga
     1921 ctgcacgttt accaacaata aggccggata ctaccttact cgcaacgccg gattccgaag
     1981 cttcgtaggt gttcggggag acatttggcg cgcaaccatt gatcaagcca aagattaccg
     2041 gtagtgtgat aaataacaaa attcttttca tattacacct tcataaataa aaatagcatt
     2101 caaaaccatc gcttactcga tccagtcatt attgtattac aggttgattc taaacataac
     2161 cctcttgatc ttcacacctt atacacacac cataaatata cagcgcatga tccgtcattc
     2221 gaaactgttt ttcttttgca atagcttcct gacgctgttc aatcaccgga tcaataaatt
     2281 caaccacttg ccggcatttt atacaaacta aatgatcatg atgtttaccg tcatctaatt
     2341 caaaaacaga atgcccacct tcgaaatgat gacgaattac taatcctgca gattcaaact
     2401 gcgttaaaac gcgatagacc gttgctaagc caacatcatc acccatttct agcagttttt
     2461 tataaacatc ttcagcactc aaatgcctaa tgtctgcgct ctgtaaactg gtttctaaaa
     2521 tatggaggat tttaacccga ggggctgtta ctttaagtcc tgccttcttt
//
LOCUS       DAORVT010000167         2565 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_38994_length_2565_cov_1.927490, whole genome shotgun sequence.
ACCESSION   DAORVT010000167 DAORVT010000000
VERSION     DAORVT010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38994_length_2565_cov_1.927490"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PK389_04070"
     CDS             <1..267
                     /locus_tag="PK389_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22910.1"
                     /translation="EISKAVRMPAHLGGAAYFNAMPEVGLGGRLAAATEAVSMFPGNP
                     SASLSAGGNVTAHMGFGDVVIPADAGATKEGVKATVVEERRKRY"
     gene            complement(346..1143)
                     /locus_tag="PK389_04075"
     CDS             complement(346..1143)
                     /locus_tag="PK389_04075"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HQY22911.1"
                     /translation="MRAMSMHPYLNIAITAARLAGKIITRHMDQLHKLTIFEKGVNDL
                     VTMVDKSAEEAIIETIYKAYPNHSILGEESGHHPGNEFTWVIDPLDGTMNYVHGFPQF
                     AVSIAVKLKDHLEHAVVYDPLSQDLYTASRGCGAHLNNRRIRVTDRADLNGALIGSDF
                     PYHDRNAKPHGVPHLEILQEIFHKGSDVRRIGSAALSLAYVAAGKLDGFWESDLNPWD
                     IAAGALIVREAGGFVSDFNGENGFLESGNIVAGTRKVHTELLQIIKK"
     gene            1335..1643
                     /locus_tag="PK389_04080"
     CDS             1335..1643
                     /locus_tag="PK389_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002739617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HQY22912.1"
                     /translation="MDIVSLDLENDMHMQTYTASEAKQHFGHLMDAARQHPVKIQKQG
                     RPFVVVLSEETFEALEDAYWILQAKEASEEGFVGVEESTAILNQLRKKNLNNATKSQL
                     "
     gene            1621..1893
                     /locus_tag="PK389_04085"
     CDS             1621..1893
                     /locus_tag="PK389_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006035741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HQY22913.1"
                     /translation="MLLKANFSKKSIKFLDKLTAKHFFQIDQKIEKLKKNAFPEDSAK
                     LKGSEDYFRVDIGEYRIIYHCDESILYIDLIGKRNDGEVYKKFDRL"
     gene            1938..>2565
                     /locus_tag="PK389_04090"
     CDS             1938..>2565
                     /locus_tag="PK389_04090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HQY22914.1"
                     /translation="MNNPGVKIILSHTTHPGNIGAVARAIKNMGFKHLCLVSPKIFPS
                     DEANARAAGAEDILATAEVVATIDEALADCHWVFGLSARARKIALPVLSVRNAAEKIA
                     NTLLETSTTPALKIGLLFGQEQSGLLNEELAKCHYQISIPANPDYSSLNLAQAVQIVS
                     YELRMAIINLNLDLKSPIVLKNGHNLADNELKNIEKPGDDLATIDEVER"
BASE COUNT          799 a          503 c          551 g          712 t
ORIGIN      
        1 gagatatcca aagctgtcag aatgccagca catctcggtg gtgcagctta cttcaatgcg
       61 atgcctgaag ttgggcttgg tggacgtctt gcagcagcaa ctgaagctgt aagtatgttt
      121 cctggcaatc ctagcgccag cctgagtgct ggtggtaacg ttactgcaca catgggtttt
      181 ggagatgtag ttataccggc tgatgcaggc gcgacaaaag agggagtgaa agcgacagtt
      241 gtggaagaaa gaagaaaacg gtactaattt tttaaagtca atctttttgg ggctattttt
      301 atagcccctt ttgttttatt aaaatgaatt aaatgaatta cctgtttatt tttttataat
      361 ttgcagcagt tccgtatgca cttttcgtgt tcccgctaca atattaccac tctctaaaaa
      421 cccattttcg ccattaaaat ccgatacaaa accaccggct tcgcgcacaa ttaatgcacc
      481 ggcagcaata tcccatggat taagatcaga ttcccaaaaa ccatctaatt taccagcagc
      541 aacataagcg agacttagcg ccgccgaacc aattcttcga acatccgacc ccttgtgaaa
      601 gatttcttgt aaaatctcta agtgtggaac gccatgcggt ttagcattgc gatcatgata
      661 aggaaaatca gatccaatta aagcgccatt gagatctgcg cggtcggtta cccggatgcg
      721 ccgattattg agatgcgcgc cacagcctct agaagcggtg tagagatctt gacttaaggg
      781 atcgtagaca accgcgtgtt ctaagtgatc tttaagttta acagcaatag acacggcaaa
      841 ttgcgggaaa ccatgcacat aattcatggt gccatctaag ggatcaataa cccaggtaaa
      901 ttcattgcct ggatgatgcc ccgattcttc gccgagaata ctgtgatttg gataggcttt
      961 gtaaatggtt tcgataatcg cttcttcagc gctcttgtcg accatggtta ctaagtcatt
     1021 tacgcctttt tcaaatatgg tgagcttgtg aagctgatcc atgtgacgtg taatgatttt
     1081 tccggcgagc cgggcagctg taatggcaat attcagataa ggatgcatac tcatagcacg
     1141 cattctaaca tggattttaa aatcgttaca ctagagccgc aattatagcg agtttatgtc
     1201 aatatacgaa caaatcacta tattaggata aacttgctat atatggataa agttctaaac
     1261 cctttttccc caggagatgg tacacgcctg ttgaatgcga agtgattatt gacttttcgc
     1321 acattttgtc tataatggat attgtatctt tagatttgga gaatgatatg catatgcaaa
     1381 catacacagc cagcgaagcc aagcaacact ttgggcactt aatggatgct gctagacaac
     1441 accccgttaa aatccaaaag caaggtagac cttttgtggt tgttttgtca gaggaaactt
     1501 ttgaagcatt agaagatgcg tattggattt tacaagccaa agaggcctct gaagaggggt
     1561 ttgtaggcgt agaggaaagc acagcaattt tgaatcaact cagaaaaaag aatttgaaca
     1621 atgctactaa aagccaactt tagtaaaaaa agcattaagt ttttagacaa gttgactgcc
     1681 aaacattttt ttcaaattga tcaaaaaatc gaaaaattaa aaaaaaatgc ttttccagaa
     1741 gatagcgcta agcttaaagg atccgaggat tattttagag tagatattgg agaatataga
     1801 attatctatc attgtgacga gagcattcta tatattgatc ttatagggaa acgcaatgat
     1861 ggtgaggttt ataaaaaatt tgatcgcctc tagatctcat tcagcaataa gacgcctctt
     1921 acataaaaat aatttaaatg aataatcctg gggtgaaaat cattttaagt cacacaacgc
     1981 accccggtaa tattggtgca gttgcacgtg ccataaagaa tatgggcttt aagcatttat
     2041 gcttagtttc tcccaaaatt tttccttctg atgaggccaa tgcacgcgct gctggagccg
     2101 aagatattct ggccacagca gaggttgttg caacaattga cgaagcgttg gccgattgcc
     2161 attgggtctt tggattaagc gccagagcac gcaagatcgc gctgccagta ttaagcgtga
     2221 gaaatgcggc agaaaaaatc gcaaataccc tgcttgaaac atcaacaacg ccagcattaa
     2281 agatcgggct gctgtttggc caggagcaaa gtgggctttt aaacgaagag ctggcgaaat
     2341 gccattatca gatcagtatt cccgctaatc ccgattattc ttccttgaat ttagcgcaag
     2401 ccgtgcaaat tgtgagttat gagttaagaa tggccattat aaacctcaac ttggatttaa
     2461 aatcgcctat cgtcttaaaa aacggtcata acctcgcaga taatgaacta aaaaacattg
     2521 aaaaaccagg cgatgattta gcgacgatag acgaagttga acgct
//
LOCUS       DAORVT010000168         2556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_39217_length_2556_cov_2.630948, whole genome shotgun sequence.
ACCESSION   DAORVT010000168 DAORVT010000000
VERSION     DAORVT010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39217_length_2556_cov_2.630948"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /gene="glmM"
                     /locus_tag="PK389_04095"
     CDS             <1..866
                     /gene="glmM"
                     /locus_tag="PK389_04095"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HQY22915.1"
                     /translation="EFCKSTFPSTLDLRGSKIVVDCAHGATYKIAPNVFKELGAEVVE
                     MGVSPDGFNINDNCGATCPLPLAERVVKENAHIGIALDGDGDRVIMVDHSGAVVDGDE
                     LLFIIARWYAETDRLKGGVVGTIMSNYGLEIAFKTLNIPFLRTAVGDRYILEMLKNKG
                     WILGGEPSGHILCLDANTTGDGIVSALRVLAVMTFTGRSLKELKEGMTKSPQILINVP
                     IAERTNIIDHPSITSAVSKAESRLKGRGRVLLRASGTEALVRVMVEGQDSRVVELVAE
                     ELAEVVKGAMV"
     gene            complement(908..1900)
                     /locus_tag="PK389_04100"
     CDS             complement(908..1900)
                     /locus_tag="PK389_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020561638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HQY22916.1"
                     /translation="MKHFKASIFITLFGLSAAFLWGGIHALVLTLILSVLEISLSFDN
                     AVLNSSVLQKMDTLWRHRFLTWGILIAVFVVRFLLPIIIVGFVTHLSLRDVFFLAIQK
                     PSEYAKLLEATKTTISSFGGIFLLLVFLSFLFDQKRDIHWLGRLEKKLNAIGKIKALH
                     IILALVVLIGIQASLPEQKKLGAIIAGSFGIILFVILNSLTTLLNRSVTKVKHSGLIQ
                     FLYLEVLDTSFSLDGVIGAFAITKDIVIILIGLTIGAIFVRSLTLFLVYKGTLSKYRF
                     LEHGAHYAIGALAIIMLISTKTHVSEIITGLIGVSFIGLSLLSSIRHNRQRGET"
     gene            2074..>2556
                     /locus_tag="PK389_04105"
     CDS             2074..>2556
                     /locus_tag="PK389_04105"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HQY22917.1"
                     /translation="MIANWKMQGSSEMISNFVLTLSQAKNQPAASDLEIILCPPTIYL
                     QKLQTALATHSLQEHFMLGAQNIYCESSGAFTGEIAPEMLVDIGGRTVIIGHSERRQL
                     FYEDDALIARKFRAAYHVGLIPILCVGETEAERVDGKTFEVLVCQLEAILALVPICAL
                     S"
BASE COUNT          765 a          467 c          585 g          739 t
ORIGIN      
        1 tagaattttg taaaagtact tttccatcga ctttagattt acgtggcagc aaaattgtgg
       61 ttgattgtgc acatggcgca acctataaaa ttgcgcctaa tgtttttaaa gaattgggtg
      121 cagaagttgt ggagatgggc gttagtcctg atggatttaa tattaatgat aactgtggtg
      181 caacgtgtcc tttgccgctt gcagagcgtg tcgttaaaga aaatgcgcat attggcattg
      241 ccttagatgg tgatggcgat cgcgtgatta tggtcgatca tagcggcgct gtggtcgatg
      301 gtgatgaatt attgtttatt attgcacgat ggtatgccga aaccgatcgc ttaaaaggcg
      361 gtgttgtggg tacgatcatg agtaattatg gtttagagat agcctttaaa acactgaata
      421 ttccgttttt aagaacggct gtcggcgatc gttatatttt agaaatgctg aaaaataaag
      481 gttggatttt agggggagaa ccttctggac atatcttatg tttggatgcg aatacgacag
      541 gagacggtat tgtttccgcg cttcgtgtcc tcgctgttat gacatttaca ggccgttcct
      601 taaaagagct caaagagggg atgactaaat cgccacaaat actcattaat gtgccaattg
      661 cggaaagaac caatattatt gaccatccca gcatcacgtc tgctgttagc aaagcggaat
      721 cacgtttaaa aggtcgtggt cgtgtattgc tgcgcgcctc aggcacagaa gctttggtac
      781 gcgtgatggt agaaggtcaa gattcgagag tggtggagtt ggttgccgaa gaactcgctg
      841 aggttgttaa aggcgcgatg gtttaaaatt aaaccttttt catggtcata cccgcttgat
      901 tcctctgtta ggtttcccct ctttgccgat tatggcgaat agaagacaaa agcgaaagtc
      961 caataaaact aacgccaata agccccgtga tgatttcaga aacatgggtt tttgtgctga
     1021 taagcataat aatcgctaaa gcgccgatag cataatgtgc accgtgttct aaaaatcggt
     1081 atttgctgag tgtgcctttg taaactaaaa acaaggtgag tgatcgcaca aaaatagcgc
     1141 caatggttag tccaattaaa ataatcacaa tatctttagt tatcgcaaaa gccccaataa
     1201 cgccatcgag cgaaaacgac gtatctagga cttctaaata gagaaattga ataaggccac
     1261 tgtgtttcac ttttgtcaca ctacgattta ataaggtcgt taagctgttt aaaatcacaa
     1321 aaaggataat gccgaagctg cctgcaataa ttgcacccaa ttttttttgt tcaggtaaag
     1381 aagcttgtat gccaattaaa accactaacg ctaatataat atgcagagct tttatttttc
     1441 caatagcatt taattttttt tctaaacgtc ctaaccaatg aatgtcgcgt ttttgatcaa
     1501 acaaaaacga caaaaagact aataataaaa aaatgccacc aaagctagaa atcgttgttt
     1561 ttgttgcttc aagaagtttg gcgtattcgc tgggtttctg gatcgccaaa aagaatacat
     1621 cgcgtaaact gagatgcgtc acaaagccta ctataataat gggcaataaa aaccgaacga
     1681 caaaaacagc aatcaaaatt ccccaggtta aaaagcgatg tcgccataga gtatccattt
     1741 tttgtaacac cgaagaatta agcacagcgt tatcaaacga taagctgatt tctaacacgc
     1801 tgagtattaa agttaaaacc agggcgtgaa tgccacccca taaaaatgct gcagataatc
     1861 caaacagcgt gataaagatc gaggctttaa aatgtttcat gggttaagcg tagaagagga
     1921 gcatcaaaat cgccacagag tttgttggtt aacgtagttt ttgtgataag cgtttcaagc
     1981 ttattttggc ttagtgccta agcgtgctaa tatacgctga ttaatctgtt ctgggggttt
     2041 tttgtcttgg tcacaaattt tcgaaagcct tgcgtgatag caaattggaa aatgcagggt
     2101 tcgtctgaaa tgatttcaaa ctttgttttg acattatctc aggcaaaaaa tcaacctgcg
     2161 gctagcgacc tagagattat attatgccca ccgacgattt acttacagaa actacaaaca
     2221 gctctggcga cacatagcct tcaagaacat tttatgttag gcgcacagaa catctattgt
     2281 gagtctagtg gtgcttttac cggtgaaatt gcgccagaga tgctggtgga tattgggggg
     2341 cgcaccgtta ttatcgggca ttcggagcgt cgtcagctgt tttatgagga tgatgcactg
     2401 attgcacgga agttcagagc cgcgtatcat gtagggctta ttcccatact ctgcgtaggc
     2461 gaaacagagg ctgaacgagt agacggcaag acttttgagg ttcttgtgtg ccaattggag
     2521 gcaattttag cgttagtgcc catttgtgca ttatcg
//
LOCUS       DAORVT010000169         2548 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_39439_length_2548_cov_2.543522, whole genome shotgun sequence.
ACCESSION   DAORVT010000169 DAORVT010000000
VERSION     DAORVT010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39439_length_2548_cov_2.543522"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="PK389_04110"
     CDS             complement(<1..976)
                     /locus_tag="PK389_04110"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HQY22918.1"
                     /translation="MNPKMTVTDAASCLGRSKEWVAQTLGARGLLHSKSNKEIYFGHE
                     TAKALFQFTFVPRVIAFHIVKGGTGKTSLVYEFAVRASLYGAKVLCVDMDQQGNLTEA
                     FQANAEAQERAVMIDVLADHVPLEDCIMTVAPGIDLLPSRFENSMLDEMIRAKGLGIE
                     KVYREPFQRLKKHYDLILIDCPPSLGLSVAAIALSADDLLAPVTPEKFALSGLELAHQ
                     SMTELQALHGVSIRFGVVLNKVEARTTLSQLTLKFLLKHSKFADLLLKRSVRFSQEFP
                     NAIASHGSIFDAIRSTAAKEDINALTQELLGIGIYESGYRDVLLPKAIH"
     gene            complement(1029..1367)
                     /locus_tag="PK389_04115"
     CDS             complement(1029..1367)
                     /locus_tag="PK389_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HQY22919.1"
                     /translation="MPNAKITLTGTAVQHLKKTLQKKTEALGIRFGVKNAGCSGMSYV
                     LDFALETKPEDHIFETAGIKVVVDPLSLEYVQGTEIDCVEEGLNAALKFNNPNVKSAC
                     GCGESFNIEK"
     gene            complement(1371..2537)
                     /locus_tag="PK389_04120"
     CDS             complement(1371..2537)
                     /locus_tag="PK389_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQY22920.1"
                     /translation="MLQKMIECLSADGVFGNPASQHSFGLKAKEKVEAGRKHIADLIH
                     TVPRNILFTSGATEANNLAIKGVVKFNQSRGKHIVTCKTEHKAVLDVCRQLEKEGFFL
                     TYLDPEPNGLLDINKLESALRSDTVLVSIMQVNNETGVRQNIEAIGALTRKRGIFFHV
                     DAAQSLGKIPIDVQQLPVDLMSFSAHKIYGPKGIGALYVGDRPRVRLNAEMQGGEQER
                     NMRAGTLATHQIVGFGEACRIAGDAISESDQIKVYRDRLWAGIQDLPGVILNSDFDIC
                     VLNILNVRFSTNILPGLTPPFGKGGLGGISAPFENSLLHRGVGGDVGLRGIAVSNGAA
                     CNTLTIEPSHVLRAMGLSREQADCSIRFSFGRFTTAEEITIAIEIVRKMVLQEK"
BASE COUNT          730 a          630 c          464 g          724 t
ORIGIN      
        1 aatggatcgc tttaggtaga agaacatctc tgtatccgct ctcgtatata cctataccca
       61 atagctcttg ggttaaggca tttatatctt ctttcgccgc ggtcgatcga atcgcatcaa
      121 atattgatcc gtggctggca atcgcgttag gaaattcctg agaaaaccgc acagatcttt
      181 tgagcaacaa atcggcaaat ttcgagtgtt tcaataaaaa ttttagtgtg agctgcgaca
      241 aggttgttct cgcctcaacc ttatttaaca caaccccaaa acgaatagaa accccatgca
      301 acgcttgtaa ttctgtcatc gattgatgtg ctaactctaa acccgataat gcaaattttt
      361 cgggtgtcac tggggccagc aagtcatctg ccgataatgc gatagcagca acagataacc
      421 caagactggg aggacaatca atcagaatca aatcatagtg ctttttcaaa cgctgaaacg
      481 gttcgcgata gactttttca atccctaaac ctttggctcg gatcatctca tcgagcatgc
      541 tgttttcaaa acgactgggc agtaaatcaa tacccggtgc cactgtcata atgcaatctt
      601 caagcggaac atggtctgca agcacatcga tcatcacggc tcgttcctgc gcctctgcat
      661 ttgcttggaa agcttctgtt aaattaccct gctgatccat atccacacat agtaccttgg
      721 caccataaag actcgcacga acagcaaatt cgtaaaccaa agacgtcttt cctgttccac
      781 ccttaacgat atggaatgcg ataacgcgcg gtacaaatgt aaactgaaac agcgctttgg
      841 ccgtttcatg cccaaaataa atttctttat tggatttgct atgcaaaaga ccgcgtgcac
      901 ccaacgtttg agcaacccat tctttgcttc ttccaaggca gcttgctgca tccgtgactg
      961 tcatttttgg attcatatta tcgttataga cgaaaatgca aagaatgtcc ttaatctaga
     1021 agataaactt atttttctat attaaaactc tcgccacatc cacaagcgct ttttacattg
     1081 ggattattaa atttaagtgc agcatttaga ccctcttcca cacaatcaat ctcggttcct
     1141 tgtacatatt caagactaag aggatcaact accactttaa ttcccgctgt ttcaaatatg
     1201 tgatcttcgg gtttagtctc taaggcaaaa tccaacacat acgacatgcc agagcatccg
     1261 gcatttttta ccccaaaacg gatccccaag gcttctgttt ttttttgtag tgttttctta
     1321 agatgttgta ctgcagtacc tgttaatgta attttagcat taggcatatg ttatttctcc
     1381 tgtaaaacca tttttcggac aatctctatc gcaattgtta tttcttctgc cgttgtaaaa
     1441 cgcccaaacg aaaatcgaat agaacaatca gcttgttccc gacttaagcc catcgctcgc
     1501 aatacatgcg aaggctcgat ggtcagtgta ttacaagcgg cgccgtttga aacagcaatc
     1561 ccccgcagcc ccacatcccc cccaaccccc ctgtgaagga gggagttttc aaagggagca
     1621 gaaatccccc ccaacccccc tttgccaaag gggggagtta atccgggtaa tatatttgtg
     1681 ctaaaacgaa catttaaaat attgagcacg caaatatcga aatcgctatt taagataacg
     1741 cccggcaaat cttgtatccc cgcccacaat cgatcacgat aaactttaat ctgatcgctc
     1801 tcggagattg catcgcctgc aatgcgacag gcttccccaa atccaacaat ttgatgtgtt
     1861 gccaaagtgc ctgcacgcat gttgcgctct tgctcgccac cctgcatttc tgcgtttaaa
     1921 cgaacgcgcg gcctatctcc gacataaagt gcaccaatgc ctttgggtcc ataaatttta
     1981 tgcgctgaaa aagacattaa atcaaccggc aattgctgca catcaattgg aattttaccc
     2041 agactttgcg ccgcatccac atgaaaaaaa atacctcgtt ttcgggttaa tgcaccaatc
     2101 gcttcaatat tttgcctaac acccgtttcg ttattaacct gcataataga aaccaacacg
     2161 gtatcggatc ttaatgccga ttccagttta ttgatatcca gcaaaccatt aggctctgga
     2221 tctaaataag tcagaaaaaa cccttctttt tctaattgcc tacatacatc taaaacggct
     2281 ttatgttcgg tcttacaggt aacaatgtgt ttaccacgag attgattaaa ttttacgacc
     2341 cctttaatcg caaggttatt cgcttctgtc gcacccgagg taaacaaaat attgcgcggt
     2401 acggtgtgga tcagatcagc aatgtgtttt ctgcccgctt caactttttc tttcgctttt
     2461 agcccaaaag aatgctgaga tgcaggatta ccaaatacgc catcagcgga aagacattcg
     2521 atcatttttt gaagcacgag cggatcta
//
LOCUS       DAORVT010000170         2532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_39870_length_2532_cov_3.113444, whole genome shotgun sequence.
ACCESSION   DAORVT010000170 DAORVT010000000
VERSION     DAORVT010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39870_length_2532_cov_3.113444"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PK389_04125"
     CDS             complement(<1..228)
                     /locus_tag="PK389_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22921.1"
                     /translation="MKVIKLSSFTAMFLGMAFLTGCAGGLNPERYLLNKQSFMLSEDN
                     PPAYVSGYVDGCSAGRRLAGDKRFVYRKNGAR"
     gene            320..877
                     /locus_tag="PK389_04130"
     CDS             320..877
                     /locus_tag="PK389_04130"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HQY22922.1"
                     /translation="MSDQRGFSILELCTVFLILGFLTAFAIPAFSQFLMREERTRVLD
                     RIQAAIEFAKQEAFARAKTITVCASINHRTCHASDWSAGFIIIETDEQLPHFASNLLY
                     AFPKLQHGKLNFDQFGTYLNITADGMTINNGTFVYCPKNGDRREADALIINNATRTYR
                     PTQKNPLGIFLKNAGTAEEMPLSCR"
     gene            885..1400
                     /locus_tag="PK389_04135"
     CDS             885..1400
                     /locus_tag="PK389_04135"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HQY22923.1"
                     /translation="MQHTLSKRRLAGFTTTELLIVGALIAILTTIAVPNYLTYLANNR
                     AATLAMQINAALSLAKNEALHRRLSVELCAIASAGAVRCNDTASSWGFGWQVIVLPLN
                     TIVYTYIPSTANAVSIAPASSITFSPNGFPSPQAYTITIKPPGCTQGYIISFNATAEN
                     GQLKTQTTSCS"
     gene            1430..1855
                     /locus_tag="PK389_04140"
     CDS             1430..1855
                     /locus_tag="PK389_04140"
                     /inference="COORDINATES: protein motif:HMM:NF028042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HQY22924.1"
                     /translation="MKWILKNRKNDLGFSLLELMIVVGIIAILSAIAFSSYSRYILKA
                     RRSEAMVNLVKFQAAYEEFNAQNNTYPASNTLPPAITPIPATTYYSYTSATTASSYTI
                     TATALAGSSQVNDIEGGVACSTMTINNVGTQTPAACWNQ"
     gene            complement(1902..2360)
                     /gene="lspA"
                     /locus_tag="PK389_04145"
     CDS             complement(1902..2360)
                     /gene="lspA"
                     /locus_tag="PK389_04145"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020158211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HQY22925.1"
                     /translation="MRKIGIVISAVVVGLDQFTKYLSTQHLTVVDSIKVFPGFDLALS
                     HNTGAAFGFLANAAGWQRWFFIGLALVMSSIIYVWLGRLSAKNKQEAFGLSLILGGAL
                     GNLVDRITHGYVTDFILLYYKDWQWPAFNLADSAICLGVVFLMPVLLKKT"
     gene            complement(2353..>2532)
                     /locus_tag="PK389_04150"
     CDS             complement(2353..>2532)
                     /locus_tag="PK389_04150"
                     /inference="COORDINATES: protein motif:HMM:NF018531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HQY22926.1"
                     /translation="TAKRTLIEGLWIVVSASVYPKCERCWHRREDVNSNSKYPGICER
                     CIDNIAGDGEAREYA"
BASE COUNT          788 a          595 c          452 g          697 t
ORIGIN      
        1 acgagcacca tttttgcgat acacaaaacg tttatcacct gccaatcgtc gaccagcaga
       61 acagccatcg acatatcctg aaacatacgc cggtgggtta tcttcactca gcataaaaga
      121 ctgtttattc aataaatatc gttcaggatt taaaccgcct gcgcagccgg ttaaaaatgc
      181 catgccgagg aacatagcag taaatgaact tagttttata actttcatat cttttatacc
      241 tttcatatca gacccaggta tcaaccacct gggttaaaca tccgattgtt aattaatcta
      301 aagagagtag cattttttga tgagcgatca aagagggttc tcaatactag aattatgtac
      361 agttttttta atactgggct ttctcacggc gtttgcaatt cctgctttct cacaattttt
      421 aatgcgcgaa gaacgcacca gggtactcga tagaatacag gctgccattg aattcgccaa
      481 acaagaggcc tttgctcgtg ctaaaaccat tacagtttgt gccagcatca atcatagaac
      541 ctgccacgcc agtgactgga gcgctggatt tatcattatt gaaacggacg aacaactgcc
      601 gcattttgct tcaaatctat tgtatgcttt tccaaaacta caacacggta agctgaattt
      661 tgatcaattt ggtacttact taaatataac cgcggatggc atgaccatca acaatggtac
      721 attcgtttat tgtcctaaaa atggcgatag acgagaagca gatgcgctga ttattaataa
      781 tgcaacgcga acttatcgac ctacacagaa aaatcctctg ggaatatttc tgaaaaatgc
      841 cggcaccgct gaagaaatgc cccttagctg ccgataaaat ctctatgcaa cacacgcttt
      901 ccaaacgaag acttgctggg tttacaacca ccgaactact gattgtagga gcgttaattg
      961 ctattttaac aactatcgcg gtgccaaatt atttaacata tcttgccaat aaccgcgctg
     1021 ccacacttgc gatgcaaatc aatgccgctt taagtttggc aaaaaatgag gcacttcatc
     1081 gacgactgag cgtagaactt tgcgccattg caagtgcagg tgctgttagg tgcaatgata
     1141 cagccagtag ctggggattt gggtggcaag tcatcgtact gccgctcaat acaattgttt
     1201 atacttacat tcccagcacc gcaaatgcgg taagcatcgc acccgcatcg agcattactt
     1261 tttcaccgaa tggatttcca agtcctcaag cgtataccat taccatcaaa ccgccgggtt
     1321 gcacgcaggg ctatattatt tcctttaatg caacggcaga aaatggacaa ttaaaaacgc
     1381 aaacaacctc gtgctcctaa atttaaatta agtcggagtt aacctgaata tgaaatggat
     1441 attaaaaaat cgcaaaaatg atcttggttt ttcattatta gaactcatga tcgtggttgg
     1501 cattattgct attctctctg ctatcgcttt ttcaagctat agccgctaca ttcttaaagc
     1561 cagacgttct gaagcgatgg ttaatttggt aaaatttcaa gcggcttacg aagaatttaa
     1621 tgcacaaaat aatacgtatc ctgcaagcaa tacactgccg cctgccatca cgcctatccc
     1681 tgcgacaacc tattattcct atacttcagc aacaacagcc tcaagctaca ccatcaccgc
     1741 cactgccctt gcaggcagca gccaagtaaa tgatatcgaa ggcggggttg cgtgttcaac
     1801 aatgaccatc aataatgtgg gtactcaaac gcctgctgct tgctggaacc aataaaacat
     1861 tgcattgttt aaaaaacgtc ttgtttttta tctggaatgc gttacgtttt ttttaatagg
     1921 accggcatta aaaaaacaac gcctaaacaa atagcagaat ctgccagatt aaacgcaggc
     1981 cactgccaat ctttgtaata taacaaaata aaatccgtta catatccatg tgttattcta
     2041 tctaccagat taccaagcgc tccgcctaag atcagcgata atccaaaggc ttcttgttta
     2101 tttttagctg aaaggcgccc taaccacaca taaataatac tactcatcac aagcgctagt
     2161 cctataaaaa accagcgttg ccaacccgca gcatttgcta aaaaaccaaa ggctgctcct
     2221 gtattatggc ttaaggctaa atcaaagccc ggaaatacct taattgaatc aactactgtt
     2281 aaatgctgtg tgcttaaata tttagtgaat tgatccaaac caaccacaac agcagaaata
     2341 actatcccta ttttacgcat attcacgtgc ttctccatcg ccagctatat tatcgatgca
     2401 acgctcgcag atccctggat acttcgagtt tgaattaaca tcctctcgcc tatgccaaca
     2461 gcgttcgcac tttggataaa ctgaagcaga aacaacaatc cataaaccct caataagtgt
     2521 tctctttgca gt
//
LOCUS       DAORVT010000171         2526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_40031_length_2526_cov_3.292189, whole genome shotgun sequence.
ACCESSION   DAORVT010000171 DAORVT010000000
VERSION     DAORVT010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40031_length_2526_cov_3.292189"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PK389_04155"
     CDS             <1..552
                     /locus_tag="PK389_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HQY22927.1"
                     /translation="FGIDPTVSAFVGLAILLSTNVLTWDDILKEQNAWHTFMWLTTLL
                     MMSTYLSEFGMITWFGEHMRTWISGFHWIPALGLISLIYFYTHYAFASITAHISAMYS
                     AFAVVAIATGAPPIIVILILAFLSSLCAGITHYGTGAGPVYFGTNYVKLKDWWRVGAI
                     LSIINLSIWMIAGAAWWKILGLW"
     gene            661..1071
                     /locus_tag="PK389_04160"
     CDS             661..1071
                     /locus_tag="PK389_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQY22928.1"
                     /translation="MSDMMQNTGAKPLKILFVDNSRTTRTAMSMILAREGYEVIPIAT
                     GQEAIEKLQAESFDVVVTDLYMPFMNGHEAAKLIRALPDPCKDVPIIALTASDDPRDM
                     EVCKNAGMNEFIIKSEDNKGLLDTLKTYTQKLGK"
     gene            complement(1080..1493)
                     /locus_tag="PK389_04165"
     CDS             complement(1080..1493)
                     /locus_tag="PK389_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22929.1"
                     /translation="MAFGFFLPWIRAWWFSLGLIFLFAVISYGLYDYWGSSHQLSHYY
                     SKEEAYYRQRQADFQVLLAEFRKEEFRLRLRLEHDPKDIEATWRLLDLLAIKALQDGD
                     PKLAIQYWESALDKMPEDLKSQFKEKILKFKSKGG"
     gene            complement(1729..2103)
                     /locus_tag="PK389_04170"
     CDS             complement(1729..2103)
                     /locus_tag="PK389_04170"
                     /inference="COORDINATES: protein motif:HMM:NF015853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HQY22930.1"
                     /translation="MHENRLHPNDKGEDIYPFSDPSQHELFTKVIHELRCSVCQNQNL
                     SDSMAPLAVDLRNEIYQKVRTHHSESEILEFVTHRYGAFVLYNPPLHWNTAFLWFGPV
                     LILVMGFFLFSKYVKRGSGETP"
     gene            2206..2472
                     /locus_tag="PK389_04175"
     CDS             2206..2472
                     /locus_tag="PK389_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006872808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22931.1"
                     /translation="MSKLLVSIPDKLANQFKALVPARQRSHFLVCLIKKAIQKQEDAL
                     YLCAKSVEEDVKLNQDMQNWDVTLEDGLDSQWDPKENHDKHSTW"
BASE COUNT          776 a          527 c          486 g          737 t
ORIGIN      
        1 tttggtattg atccgacggt gtctgctttt gtgggactcg ccattttgct atcgaccaat
       61 gtactgacct gggacgatat cctaaaagaa caaaatgctt ggcatacgtt tatgtggctc
      121 acaacgcttt taatgatgtc gacttatttg tcagaatttg ggatgattac ttggtttggt
      181 gaacatatgc ggacatggat cagcggtttc cattggatcc ccgctcttgg cttgattagc
      241 cttatctatt tttatacgca ttatgctttt gcgagcatta cggcgcatat cagtgccatg
      301 tacagcgcgt tcgcagtagt tgccattgcc acaggcgctc ctcctatcat cgttatctta
      361 atactcgctt ttctgtcgag cctttgtgcc ggtattacac actacggtac tggcgccggg
      421 cccgtctatt ttggtactaa ttacgtcaag ctaaaagatt ggtggcgtgt gggtgctatt
      481 ttaagcatta ttaacctcag tatctggatg atcgctggcg ccgcctggtg gaaaatctta
      541 ggtttgtggt aagcataatt acgtcaaatg ctctaaaaaa agctcactaa aaatgtatgc
      601 ccataattat tcaaatctct attcttggca aaaatctcgg taatagacta gaatgaagtc
      661 atgagcgata tgatgcaaaa tacgggtgcc aagcctttaa aaattctgtt tgtcgacaac
      721 agcagaacca caagaactgc tatgtcaatg atccttgcta gagagggtta tgaagttatt
      781 cctattgcaa cgggtcaaga ggccattgaa aagctacaag cagagtcctt cgatgtggtt
      841 gttacagatc tctatatgcc ctttatgaac ggccacgaag cagccaagct gattcgcgca
      901 cttcctgatc cttgtaaaga tgttcccatt attgcattaa cggccagcga tgatcccagg
      961 gatatggaag tttgtaaaaa tgcagggatg aatgaattta ttattaaatc agaagacaac
     1021 aaaggattac tcgatacgct taaaacttat acccaaaaac tcgggaaata gcttaaaact
     1081 cagcccccct ttgatttgaa ttttaagatt ttttctttaa attgcgactt taaatcctct
     1141 ggcattttat ctaaagcgct ttcccaatac tgtatcgcca atttaggatc cccatcctgc
     1201 agtgctttaa tggccagtaa atctaacaat cgccacgtcg cctcaatgtc tttgggatcg
     1261 tgttctaacc ttaaccgtag ccgaaattct tcttttcgaa attcagctaa taacacttga
     1321 aaatccgctt gtctctggcg atagtacgcc tcttctttag aataataatg ggataactga
     1381 tggctactac cccagtaatc gtacaatcca taactaataa cagcgaatag aaaaattaaa
     1441 ccgagagaga accaccaagc acgtatccac ggcaaaaaga acccgaaggc caaaatcaat
     1501 aatagggctg tgatagacca aaatatgtag tacatataac cctacaatac acatgaggtc
     1561 aaacctcatt gatagaagcc ttcactctaa caagaaactg gtttacaatc tatcctccta
     1621 accaccgtat cgggagctaa acccatgaac gccaagtctt gccgtccaga aaagcttata
     1681 tttggtgctt tgcgacgtat ctaggcgaag cataaacagc ctacaggact acggagtttc
     1741 gccgcttcct ctttttacat atttgctaaa aagaaaaaat cccatgacta aaattaatac
     1801 agggccaaac cataaaaacg ctgtattcca gtgtaaagga gggttataca acacaaatgc
     1861 accataacga tgggtaacaa attcaaggat ctctgattct gaatggtgcg ttcgtacttt
     1921 ttggtagatt tcatttctca ggtcaacagc caatggcgcc atggaatcag ataagttttg
     1981 attttggcaa acactacagc gcaattcatg aataaccttg gtaaacaact catgttggct
     2041 cggatcgcta aaaggataaa tatcttctcc cttatcgtta ggatgtaatc tattttcgtg
     2101 cataccctct cactttgact cttaacttca aaattaatca cttacactct agcattatta
     2161 ctagatattg gatatactat attatatata tagcaggaag tgaacatgag caagctatta
     2221 gtttcaatac cagacaaatt ggcaaaccaa tttaaagctt tggttcccgc gcgccaacgc
     2281 agtcattttt tagtctgttt aataaaaaaa gctattcaaa aacaagaaga tgcgctttat
     2341 ctttgtgcca aatctgtcga ggaagatgtc aagcttaatc aagacatgca aaattgggat
     2401 gtcactttag aagacggttt agatagccaa tgggatccaa aggaaaatca tgataaacat
     2461 tcaacgtggt gaaatttatt gggtagattt aaatccaggt catggttccg aaattaataa
     2521 aattag
//
LOCUS       DAORVT010000172         2514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_40387_length_2514_cov_2.836112, whole genome shotgun sequence.
ACCESSION   DAORVT010000172 DAORVT010000000
VERSION     DAORVT010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40387_length_2514_cov_2.836112"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..251
                     /locus_tag="PK389_04180"
     CDS             99..251
                     /locus_tag="PK389_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22932.1"
                     /translation="MQFQNYLIDAVNDVLDWDISDEAFTEAVHVQACLMARVNPDEMM
                     TMFITD"
     gene            341..757
                     /locus_tag="PK389_04185"
     CDS             341..757
                     /locus_tag="PK389_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HQY22933.1"
                     /translation="MNTKTITRKLLPINQRINHTAKIFGVHFPMRFEPFVYHITETIA
                     PEYNGGYWDFYTLSNGAFYMAPDSDKSFQVICENGFEGMLSGDALGITVCLYAYSHLS
                     FGDQNAFTDTCARQYHWLRDYMLGHAEAREILGAVD"
     gene            1178..1306
                     /locus_tag="PK389_04190"
     CDS             1178..1306
                     /locus_tag="PK389_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22934.1"
                     /translation="MFNAEDDIIPKLEASCTKEEAVAKMLGWLQGPYSDEFQVAYE"
     gene            1392..2078
                     /locus_tag="PK389_04195"
     CDS             1392..2078
                     /locus_tag="PK389_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22935.1"
                     /translation="MESHYDVPDDIDIESECVFCLSEYLNEEYDFLECDYNLAKHNKE
                     PDDIIATAFEKLNQFKKLISKAKEYANYFDDEIAKGESSKIRIHKPDSSFTPHYTVVS
                     IHEWAKETLKFPNCSLDSFLKNNTDLSEAPENDDQSNQEEWKSPTSAETFHTTFAFLV
                     ESFALTSAAYNIDGKLNVDAIAKHLQECAEKANQGHSLTGQSSNNIRKMIPNSLKIKK
                     KVLHKRGDRK"
     gene            2287..>2514
                     /locus_tag="PK389_04200"
     CDS             2287..>2514
                     /locus_tag="PK389_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22936.1"
                     /translation="MIEKEITVPPISEHESEPEALTISTTPLNIQNNEFIAAIFQQIS
                     PNAFAAVCSKSGNPEIGGWPSWKVTGQSAQLY"
BASE COUNT          776 a          541 c          518 g          679 t
ORIGIN      
        1 gctgttcatt atcaatccca agttcatggc gcttctgttg ctctggagcg tccttgccca
       61 tttcttctat cacaacaata cataaaggag tgtttgccat gcaattccaa aactatctaa
      121 ttgatgcagt caatgatgtg cttgactggg atatttctga tgaagctttc actgaagctg
      181 ttcatgttca ggcgtgtttg atggcccgag tcaatcccga tgaaatgatg acaatgttta
      241 ttactgactg agtcatcgct gtctctctgt ttcacatcca tcaattcgga gtggtgcatt
      301 caattgcgct gctccgtttc tttttttaca ggagtttttt atgaacacta aaaccattac
      361 acgtaagctt ttgccaatca atcaacgcat caatcacacg gccaagatct tcggtgtcca
      421 ctttccaatg cgctttgaac cattcgttta ccatatcact gagacaatcg cgccggaata
      481 caatggcggt tactgggatt tttatacatt gagtaacggt gctttctata tggcgccaga
      541 ttcagataaa tcgtttcagg tgatctgtga gaatggcttt gaagggatgc tctccggaga
      601 tgcactgggc attactgttt gtctgtatgc ctatagtcat ttatcgtttg gggatcagaa
      661 tgcatttact gatacctgtg cccggcagta tcattggctg cgggattata tgttggggca
      721 tgctgaggct cgggagattc tgggagctgt tgactgacac tttctggggg tggaggggag
      781 gcaaaggggg caaaggaggt gaaagccggg ggtaaaggag gtggccggaa tgccttgccc
      841 agcatgagat ttcattattt ttaccacctt taccgccttt tttcccccgt ccgacggaga
      901 aaacatcacg gagaattttc gggatttgca ccggaaattt ccgcgttggc tttttcgcac
      961 agcgcgccgg aaaaagatgg taaaggcgga gaaattggcg aagatccatg cagggtatga
     1021 aaagcaggct accacctttg cgccaccttt cttccccttc acctccttct tgagtttcgg
     1081 tttcggcagt gcccatgtcg attaacttaa ccatcattac catagacagg tttgtatata
     1141 ctggccgcaa gttaccttat ttataggaat caacacaatg ttcaatgctg aagacgacat
     1201 cattcccaag ctcgaagcaa gctgcacaaa ggaagaagcc gtagccaaaa tgctgggatg
     1261 gttgcaaggg ccatatagcg atgagtttca ggttgcatat gaataaaatt ataatcactt
     1321 tatcaacgag ctgttcaaga gacgaggcca tagcgctatt aatcggctta ggggatcacc
     1381 gatatatacc gatggaaagt cactatgatg ttccagatga tatagatata gaatcagagt
     1441 gcgtcttctg cttgagcgaa tacctcaatg aagaatatga ttttttagaa tgcgactata
     1501 acctggccaa gcataacaaa gaaccagatg acataattgc cacggcattt gagaaattaa
     1561 atcaatttaa gaaattaatt tcaaaagcca aagaatacgc taactacttt gatgatgaga
     1621 ttgctaaagg cgaatcatct aagataagaa tccacaagcc cgatagttca tttactcctc
     1681 actacacagt cgtaagcatt catgagtggg ctaaagaaac actgaaattt cctaattgca
     1741 gtctggattc ctttcttaaa aataacacag atttatctga agctccggaa aacgatgatc
     1801 aatccaacca agaggaatgg aaaagcccca catctgcaga aacttttcac acaacatttg
     1861 cttttctcgt tgaatcattt gctcttacaa gcgcagcgta caatatcgac ggcaaactga
     1921 atgttgatgc catagccaaa cacttacaag agtgcgcaga aaaagccaat caaggccata
     1981 gcttgaccgg ccagagttct aataatataa gaaaaatgat cccaaactct cttaaaatta
     2041 aaaaaaaagt acttcataaa agaggtgata gaaaataatt accagagaca aattaaaatt
     2101 accaattaaa ttgtgtgcat taatgacaac acataggtat taattcacaa tcaataagtt
     2161 atcaccatta caagctcact aaaagattaa atccacgctt aaaaaaatta ccaaaaggta
     2221 attccgccat tcccaaacca accctgaaaa aattgcgact tcttcaaccg tttgaaagga
     2281 gccgcaatga tagaaaaaga aattaccgtt ccaccaattt ccgagcatga gagcgagccg
     2341 gaagcactta ccatctcaac aacgccacta aatattcaga acaatgaatt tatcgctgca
     2401 atatttcaac agatctcgcc aaacgcattt gcggctgttt gctccaaatc cggaaatcct
     2461 gaaatcggcg gctggccatc ttggaaagtc acagggcaat cagcacaatt atat
//
LOCUS       DAORVT010000173         2506 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_40619_length_2506_cov_4.106079, whole genome shotgun sequence.
ACCESSION   DAORVT010000173 DAORVT010000000
VERSION     DAORVT010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40619_length_2506_cov_4.106079"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="PK389_04205"
     CDS             complement(<1..1135)
                     /locus_tag="PK389_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22937.1"
                     /translation="MTKKWLDKLGSSKTRALVLLLSIVLFLIVVAVIFSSGKPNPLKT
                     DESRTTKIPQITAIPGGATSEKYQELQEADNKRIAEQAKKKGGSAVATIIGNRDNDLL
                     SKKESFGIEGEFLKAGECKCPTPTAPSVKPACPKDLELALKSIINNKEAAKKILMQCP
                     AMAKLLAERNPEMFKQLMLENPDLAKLIGDTYPEVLKKLMESDPAFAAQLAKTNPALV
                     KNLITNDPDFAETMAKANPDMIKDLNRGDLEFSKQLAKNNPALVKKMMLSDPEFAKLM
                     GKNNPTMIKELMKGDPAFARALAKSNPTLVKELMKNDAAFADLMGEQNPEMVKQLMLD
                     DIEFAKVMARNNPNMVETLMENDPAFAKALLTKIPDVKTILDSS"
     gene            complement(1139..2107)
                     /locus_tag="PK389_04210"
     CDS             complement(1139..2107)
                     /locus_tag="PK389_04210"
                     /inference="COORDINATES: protein motif:HMM:NF023713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotH/IcmK family type IV secretion protein"
                     /protein_id="HQY22938.1"
                     /translation="MKRKNYFSKVALLTLCFIHMSSPLFAAEVTNAQLNALVQQAQDK
                     RALQENGGLATASPGDTSENKPSTLRDEAFKQLLDKISPLTPAQIIQMRKQEDKTQQA
                     IATTPSAPPRPVSSTLTIDLSPGVTPPVVRLSAGFVSSLVFVDSTGQAWPISDYSLGN
                     PKNFNIQWDRKTNTLFIQSATTYSSANLALRLIGLDTPVMLSLVSGQKEVDYRVDCQV
                     QGRGPNATMPLVGDGLPTITPSLVSVLDGVPPPGSQELAVSGGYGRAWLSKGKLIFRT
                     QLTVLSPAWSATVSSPDGTRVYEMANTPLILASQNGRTVKIELKGF"
     gene            complement(2133..>2506)
                     /locus_tag="PK389_04215"
     CDS             complement(2133..>2506)
                     /locus_tag="PK389_04215"
                     /inference="COORDINATES: protein motif:HMM:NF022835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HQY22939.1"
                     /translation="TFDFFNHKDQLNAMKDYFTADGFDSFIASLQETGVLTTIVEKKL
                     ILSAVAIGPAIVLTEERVGSKHNWRIQVPLIVRYQSANVDETRTQVVEVLVTQVSTED
                     APKGIGIAQYIAREVGPELTR"
BASE COUNT          613 a          513 c          554 g          826 t
ORIGIN      
        1 ggctggaatc aaggatggtc tttacatccg gtatttttgt caagagcgct ttggcaaaag
       61 cggggtcatt ttccatcaat gtttctacca tattggggtt atttcgtgcc atgacttttg
      121 caaattcaat atcatctaac atgagttgtt ttaccatttc gggattttgt tcacccatca
      181 gatcagcaaa agccgcatcg tttttcatta attctttgac gagggtgggg ttgcttttgg
      241 caagtgctcg tgcaaatgca ggatcgccct tcatgagttc tttaatcata gtgggattgt
      301 tttttcccat tagttttgca aattctgggt cacttagcat catttttttg actaaagctg
      361 gattgttttt agccaattgt ttagaaaatt cgaggtcccc tctatttaga tctttaatca
      421 tgtctggatt tgcttttgcc atcgtttcgg caaaatcagg atcattcgtt ataagatttt
      481 tgaccaacgc agggtttgtt tttgccaatt gtgcggcaaa tgcaggatcc gactccatta
      541 attttttaag cacttcagga taggtgtctc caatcagctt agcgaggtca ggattctcta
      601 gcatcaattg tttaaacatt tctggatttc tttcagccaa taattttgcc atggcagggc
      661 attgcattaa tatttttttt gcagcttctt tattgttaat aatggatttc agcgcgagct
      721 ctagatcttt ggggcatgca ggtttaaccg atggtgcagt gggtgttggg catttacatt
      781 caccggcttt taaaaattca ccttcaatac caaaagattc ttttttggag agcaaatcat
      841 tgtctctatt gccgataatc gttgcaacag cgctgccgcc cttcttttta gcttgttctg
      901 cgatacgttt attgtcggct tcttgcagtt cttggtattt ttcggaagtg gcgccacctg
      961 gaatagcggt tatttgtggt attttggtgg tgcgggattc atccgttttt aaggggttag
     1021 gtttgccgct tgaaaaaata acggcaacaa cgatcaggaa caggacgatg ctgagcaata
     1081 aaacgagggc tctggtttta ctggagccta atttgtctaa ccattttttt gtcatgcact
     1141 aaaatccttt cagctcgatt ttaacagttc ttccgttctg tgaggctaaa attaaagggg
     1201 tgttagccat ttcataaaca cgggtaccat ctgggctgga tacggtagcg ctccaggctg
     1261 gtgataatac ggtcagctgt gttcgaaaaa tcaatttacc tttcgataac catgcgcgac
     1321 cataaccacc actcacggct aattcttggc tgccaggggg cggaacacca tctaaaacac
     1381 ttaccaggct tggggtgatt gttggtagcc catctccaac aaggggcatt gtggcattgg
     1441 gacctcggcc ttgaacctga caatcgacgc gataatccac ctctttctgt ccagaaacca
     1501 gggatagcat aacaggcgtg tctaatccga ttaatcgtag cgctaagtta gcgctgctat
     1561 aggttgtcgc gctttgaata aacagcgtat tggtttttct atcccattgg atattaaaat
     1621 ttttcgggtt ccccaagctg tagtccgaaa tgggccatgc ttgtccggtt gagtccacaa
     1681 ataccaatga agaaacaaaa cctgcggata accttacaac aggtggggtt acacctgggg
     1741 ataagtcaat tgtgagcgtt gaagagacag gtcgaggggg cgcgcttggc gtggtcgcaa
     1801 tggcttgttg ggttttatct tcctgctttc gcatctggat aatttgagca ggggttaaag
     1861 gagatatttt atctaataat tgtttgaacg cttcatcacg caaggtggaa ggtttattct
     1921 cggatgtgtc tccaggggac gcagtcgcca atccgccgtt ttcttgtaat gctcttttgt
     1981 cttgtgcttg ctgaaccaac gcatttaatt gtgcgtttgt gacttctgca gcgaataggg
     2041 ggctgctcat gtgtataaaa cacaaggtca gcaaagcaac ttttgagaaa taattttttc
     2101 ttttcatatt tacaatacat ttactttgtc tattaacgag taagttcagg accgacctcc
     2161 cgcgcgatgt attgggcaat cccaatacct tttggcgcat cttctgttga tacttgagtc
     2221 actaagacct caaccacttg cgttcgtgtt tcatccacgt ttgcactttg ataacgtaca
     2281 atcaacggta cttggattct ccagttgtgt ttactcccta ccctttcttc agttaagaca
     2341 atagcgggtc caatcgcaac tgcggagagt attaattttt tttctacaat cgttgttagg
     2401 acacccgttt cttgcaatga cgctataaaa ctatcaaaac catctgccgt aaaataatct
     2461 ttcatggcat ttaattggtc tttatgatta aaaaaatcaa atgtaa
//
LOCUS       DAORVT010000174         2504 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_40681_length_2504_cov_4.260514, whole genome shotgun sequence.
ACCESSION   DAORVT010000174 DAORVT010000000
VERSION     DAORVT010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40681_length_2504_cov_4.260514"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PK389_04220"
     CDS             <1..247
                     /locus_tag="PK389_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HQY22940.1"
                     /translation="TDFSLDENRHILEESFEIVIKNHKDQDVKVFIQENFYRTANWEI
                     LKNSNPFEKINSGTIKFPLVVKASGEAKVQYTARYTW"
     gene            complement(251..>2504)
                     /locus_tag="PK389_04225"
     CDS             complement(251..>2504)
                     /locus_tag="PK389_04225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HQY22941.1"
                     /translation="VDMLKDMRLAVPLKIYANDGKLISEFGEERRTPIPLTQVPKDLI
                     HAVLATEDRRFYEHPGVDFRGLLRAGVTLLTNSSIKQGGSTITMQVARNFFLTRQKTF
                     IRKLSKILLALKIEKGLTKDEILELYLNKIYFGKRAYGIASAAAVYYGTTVDKLSLDQ
                     IAMLAGLPQAPSSINPLHDPDAAFKRRKHVLERMLSYNFITPEQFKTAVDAPLPTKSH
                     AHTPEIEAPYVAEMVRQDLYARYGNAIYTLGYEVYTTIDSQQQLAANLALKKALLKYD
                     QRHGYRKTKIHFKLSRNKTPEQELENWTQELRDIPAYGDILPGVVTRIDNANISVLLQ
                     DGRYIEIPWAGLSWARPQLKGARVGASPKKPSDVVAIGDVIYTEATQDQTWKMVQIPE
                     VGGAIVALAPDTGSILAMVGGFDYRLSSFNRVTQAERQPGSGFKPFLWAAALAQGYTL
                     ATKINDAPLSFTDPVSGVVWRPHNSTNKFYGPTSLRKSLARSQNMVAIRLLQAVGVKK
                     FLEFSKNFGFNPEKFPPYLPLALGAGELTPLEVAAGYCVFANGGNLVTPNLITRIKDY
                     QGNLVYEAPSPTSTVSIGPQIAYLMTAALQDTIQSSVGTGQRAKSLGRADLAGKTGTT
                     NEWFDAWYSGFNRDVVVTTWVGFDDPRTLGEKEFGGVVALPMWMSFMEIVLKDKPEHT
                     LEPPPGIVSVKIDPNTGLLAHEDQPGAVAEFFTEDTLPTGRGSSAGMFDEENGGGSDG
                     GYGTSVEALF"
BASE COUNT          700 a          534 c          537 g          733 t
ORIGIN      
        1 aacggatttt agcttagatg agaatcgaca tatcctggaa gaatcttttg aaattgtgat
       61 taaaaatcat aaagatcagg atgtgaaagt ttttattcag gaaaatttct atagaacagc
      121 caattgggaa attctaaaaa attccaaccc gtttgaaaaa atcaattcag gtaccataaa
      181 atttccgtta gtcgtaaagg caagcggtga ggcaaaagtg caatatactg cacgatacac
      241 gtggtaattt ttaaaacaac gcctctacgc tggtgccata accaccgtca ctgcctccgc
      301 cattctcttc atcaaacatt cctgctgagg atcctcttcc ggtgggtaag gtatcttccg
      361 taaaaaattc agccacagcg ccaggttgat cttcgtgtgc gagcaatccc gtattgggat
      421 caattttaac gcttacaatg cccggaggcg gttctaaagt gtgctctggt ttatctttaa
      481 ggactatttc cataaaagac atccacatcg gcaaggcgac aactccaccg aactcttttt
      541 cgcctaaggt acggggatca tcaaatccta cccaagttgt tacaacgaca tcgcgattaa
      601 agccagagta ccaggcatca aaccattcat tggttgtacc tgtttttcct gctaaatcgg
      661 cgcgtcctag gcttttggcg cgttgtcctg taccgacact gctttgaatc gtatcttgca
      721 atgcagctgt cattaaatag gcaatttgag gaccaatgct aaccgtactg gttggagaag
      781 gcgcttcata cactaaattg ccttgatagt cttttattct tgtaattaaa ttgggtgtta
      841 caagattgcc accattcgca aatacacaat agccagccgc aacctcgagc ggcgttaatt
      901 cgccagcgcc gagtgccaag ggtaaatagg gtgggaattt ttcaggatta aatccaaaat
      961 tctttgaaaa ttctagaaat tttttaacac ccacggcttg taataatcga atcgctacca
     1021 tgttttgtga gcgtgcaaga cttttacgca aactcgttgg accataaaat ttgttggtag
     1081 aattgtgtgg gcgccaaaca accccagaaa caggatcagt aaacgacaaa ggtgcatcat
     1141 taatcttggt tgccaaggta tagccttgtg ctaaggcggc agcccaaagg aagggtttaa
     1201 agcctgaacc gggttgtctt tctgcttgtg taacgcgatt aaaactactg agtctataat
     1261 caaatccacc gaccatggcg agtatagaac ccgtatcggg cgctaatgcg acaattgcac
     1321 cgcccacttc tgggatttga accattttcc aagtttgatc ttgtgtggct tccgtataga
     1381 tcacatcacc tattgcgaca acatcagaag gttttttagg cgaagcgcca acgcgagcgc
     1441 ctttcaattg tggtcttgcc cacgataatc ccgcccacgg gatttcaata tacctgccat
     1501 cttgcagtag aaccgaaatg tttgcattat cgatgcgtgt aacaacgcct ggtaaaatat
     1561 cgccataggc agggatgtct ctcagttctt gagtccaatt ctcgagttct tgttcaggtg
     1621 ttttgttgcg cgataattta aaatgaattt tggttttgcg ataaccatga cgttgatcat
     1681 attttaacag tgcttttttt aaggccaaat ttgctgctaa ttgttgttgg ctatctatcg
     1741 tggtatagac ttcgtaacct agagtataga ttgcgttacc ataacgagca tagagatctt
     1801 gtcgtaccat ttctgcaaca tatggcgctt caatttcggg cgtatgcgca tgtgattttg
     1861 tcggcagtgg tgcgtcaacg gcggttttaa attgttctgg cgtaataaag ttatacgata
     1921 acatgcgttc taaaacgtgt tttcgacgct tgaaagcggc atcgggatca tgtaacgggt
     1981 taatgctaga aggcgcttgt ggcagtcccg ctaacatggc aatttgatct aacgagagtt
     2041 tatcaactgt ggtgccataa taaacagctg ccgcggaagc gatgccatag gcacgttttc
     2101 caaaataaat tttgttcaaa tagagttcca atatctcatc ttttgtgagt cccttttcaa
     2161 ttttaagtgc aagcaatatt ttagaaagct ttctaataaa tgtcttctgt cgtgttaaga
     2221 aaaagtttcg agcaacttgc atggtgatag tgctaccacc ttgtttaata gatgaattgg
     2281 tcagtaaagt aacacctgca cgtaataaac ctcgaaaatc gacacccgga tgttcataaa
     2341 aacgcctgtc ttctgtcgcg agaaccgcat gaataagatc tttcggcact tgggttaggg
     2401 gaataggcgt acgtcgctct tctccaaatt cagaaatcag ttttccgtca ttcgcgtaga
     2461 ttttaagcgg tacagcaaga cgcatgtctt ttagcatatc aacc
//
LOCUS       DAORVT010000175         2493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_41034_length_2493_cov_3.463905, whole genome shotgun sequence.
ACCESSION   DAORVT010000175 DAORVT010000000
VERSION     DAORVT010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41034_length_2493_cov_3.463905"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1879
                     /locus_tag="PK389_04230"
     CDS             <1..1879
                     /locus_tag="PK389_04230"
                     /inference="COORDINATES: protein motif:HMM:NF009586.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HQY22942.1"
                     /translation="GFSALAHSAVVQKIATHSLTAAITAMVPNSLGPAELLINYGTQH
                     QKQHYLPRLARGEEIPCFALTSPEAGSDASAITDVGIVCEGEFLGKKILGMKLTWDKR
                     YITLAPVATVLGLAFKLCDPNKLLGEKEALGITVCLIPTTHPGVECGLRHFPLHQSFM
                     NGPTRGKDVFVPLDWIIGGEQMIGKGWHMLVECLSAGRGISLPALTAASSKIAYRTTG
                     AYARIRKQFNTSIGKFEGVAALMANIAGNTYMIEAARLLTLGALDQKIRPAIVTAIAK
                     YHLTEMGRKVVNDAMDIHGGRGIMLGANNYLANAYASIPISITVEGANVLTRNLIIFG
                     QGVIRCHPYVQAEMKAMVNPNEAMGILDFDRIVFKHLQYTLSNKARILFHSLTAGGFC
                     RAPKTRCFKHYYRQLSRMAVALSFISDVALMLLGGKLKRCERLSARLGDVLSYLYLAS
                     AVLKYFQDVGNKKDDLASVNWCVQTCLYQIQKAFLEFFRNFPNRFIGSCLRFLVFPYG
                     HAYQRPNDILENHLAAEMMVPGDFRNRLTQHCYFGKSNRNPVTQLDETLDKMIAAEAA
                     SLKLEALVKQGSIPKKLSLTESIAKALALQALTQEEARLLGEFDTARQEAIRVDEFEA
                     L"
     gene            1989..>2493
                     /locus_tag="PK389_04235"
     CDS             1989..>2493
                     /locus_tag="PK389_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HQY22943.1"
                     /translation="MKNKLKDVYVVDGLRTPFTKAMGKPNPLSAADLAVAAAGPLLAR
                     QPFKPSEINEVIAGCVAPGADEVNIARIIALRSGCGIEVPAFTVQRNCASGLQALESA
                     ALNIRSGKSELVLAGGTECMSRAPLLYPNEMAIFLAQLRSRKGLIDQLKSLLQFRPYF
                     LKPIFSLL"
BASE COUNT          667 a          459 c          620 g          747 t
ORIGIN      
        1 tggttttagt gcattggcgc attctgcagt tgtacaaaag attgcaaccc atagtttaac
       61 cgctgctatt actgccatgg tgccgaattc attggggccg gctgaattgc tgatcaatta
      121 cggcacacag catcagaagc aacattactt accgcggttg gcgcgtggcg aagaaatacc
      181 ctgttttgct ttaacctcac cagaagccgg atcagatgcc agtgcgatta cagatgtcgg
      241 tattgtgtgt gaaggtgagt ttttaggaaa gaaaatctta gggatgaaat taacctggga
      301 taagcgctac attaccttag caccagttgc gacagtgtta ggtttagcat ttaaattgtg
      361 tgatcccaac aaattattag gcgaaaaaga agccttgggt ataaccgttt gtttaatccc
      421 aacaactcat cctggtgtag agtgtggtct tcgacatttt cctttacatc aatcctttat
      481 gaatggtccc acgcgcggta aggatgtgtt tgtgcctttg gattggatta ttggcggtga
      541 gcagatgatt ggtaaaggct ggcatatgtt ggtcgaatgt ttatcggcag gtagagggat
      601 ctccttgcct gcattaacag cagcttcttc aaaaattgct tatcgtacga caggcgctta
      661 cgcccgtatc cgaaaacaat ttaatacctc tattggtaaa tttgaaggcg ttgcagcatt
      721 aatggcaaat attgctggca atacctatat gattgaagca gccagattgt taacgctcgg
      781 ggccttagat caaaaaattc gcccggctat cgtgacggcc attgctaaat accacttaac
      841 agagatgggt cgtaaggttg tgaatgatgc tatggatatc cacggggggc gtggtattat
      901 gttaggtgcc aataactatt tggcgaatgc ttacgcaagc attcccatta gcatcacggt
      961 tgaaggagcc aatgtattaa cccgaaattt gattattttt gggcagggcg ttattcgatg
     1021 ccatccttat gttcaggcag agatgaaggc aatggttaac cccaatgaag cgatgggtat
     1081 tttggacttc gatcggattg tttttaagca tttgcaatac acattgtcta ataaggcaag
     1141 aatacttttt cattcattaa ctgccggtgg tttttgtcgt gccccgaaaa cacgatgttt
     1201 taaacattat tatcggcagt tgagtcgtat ggctgttgct ttatccttta tttccgatgt
     1261 tgcattaatg ttgttaggcg gtaagttaaa gcgttgtgag cgattatcgg ctcggttagg
     1321 cgatgtctta agctatttgt acttagcctc ggctgttttg aaatattttc aagatgttgg
     1381 caataaaaag gacgatttgg cttcggtaaa ctggtgtgtg caaacctgtt tgtaccaaat
     1441 ccaaaaggcg tttttagaat ttttcagaaa cttccccaac cggtttatcg gcagttgtct
     1501 tcgtttttta gtgtttcctt atggccatgc ttatcagcga cccaatgata ttttagagaa
     1561 tcatttggcc gcagaaatga tggtaccggg ggactttaga aatcgtttaa cgcagcattg
     1621 ttattttggt aaaagcaatc gcaatcccgt tacccagctg gacgagactt tagataaaat
     1681 gattgctgct gaagcggctt cactcaaatt ggaagctttg gttaagcagg ggagtattcc
     1741 caaaaaactg agcttaaccg aaagtattgc aaaggcactc gcgcttcaag cgcttacgca
     1801 agaagaagcg cgtttgttag gtgagtttga tacggcgaga caagaagcca ttcgcgtgga
     1861 tgaatttgag gctctgtaaa aaactttcgc ttttgagcga ggtttgtttg agcagagcga
     1921 gttaagcgag cgagaaaaga ggaagttttt acagagccta atttaagggt attgagtagg
     1981 aagatcgtat gaaaaacaag ctgaaagatg tttatgtggt agacggattg cgaacacctt
     2041 ttactaaggc tatgggaaaa ccgaatccct tatctgctgc ggatttagcc gttgcagctg
     2101 cgggcccctt attggcgcgt cagccgttta aaccgagcga gattaatgaa gtgattgcgg
     2161 gatgtgtggc gcctggtgca gatgaagtga atattgcgcg tattattgcc ttaagatcgg
     2221 gatgtggtat tgaggttccc gcttttaccg tacaaagaaa ttgtgcatcg gggttacagg
     2281 cattagaaag tgccgcgctt aatattagaa gcggaaaatc agagcttgta ctggcgggtg
     2341 gaacagagtg tatgagtcga gcgcccttat tgtatcctaa cgagatggct atttttttag
     2401 cacagctcag atctcgaaaa gggcttatcg atcaattaaa atcattgcta cagtttagac
     2461 cctatttttt aaaacccatt ttttcattgt tga
//
LOCUS       DAORVT010000176         2493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_41035_length_2493_cov_3.179245, whole genome shotgun sequence.
ACCESSION   DAORVT010000176 DAORVT010000000
VERSION     DAORVT010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41035_length_2493_cov_3.179245"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="PK389_04240"
     CDS             complement(<1..784)
                     /locus_tag="PK389_04240"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HQY22944.1"
                     /translation="MPFSLPDLNLTFTHFMDALKDFDFFFILRLSSYIVVSILLTLYI
                     CRLVGKVSARHFSAHHSQLLRRITFYVGLALSFVFPLRAFGVNVTGFLEAAGIAAGIV
                     TAAVAFASQTSISNFLSGMFLIVEKPFEVGDFVRINDTLGEVLSIDLLSVKIRTKDNI
                     FVRVPNESLLKSQFENVTRFPIRRCDIKLHIGFNEDLQKLKKILLEVAKQNSLCLVSP
                     APELAFLEFGEAALQLQFSVWGKQTSFNSLQTSIKTDIQTAFN"
     gene            complement(789..1610)
                     /locus_tag="PK389_04245"
     CDS             complement(789..1610)
                     /locus_tag="PK389_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22945.1"
                     /translation="MKYWLLEKLKNLPFIKKKAKAAPIQSTDEEDLAEQELWWGTFLI
                     EEEQSRFFKIGQLVTCVDRFNHKWHITTYHEGEQPFKTFAAQTSNTINLKPMLPDRAL
                     LSLLDRPCYIPLGETLRVYISVPLWIQISAGNPITILDEFATETLADTWFGKSTRAGE
                     LCYNNPIHGSPRLEDLPQDSTHAIIPVSIENRTKGTNLLHELKIPLDNLALYSDKKNR
                     LWTEDVHLCQEDTNSLETLTVKNAPLPLSDLQLVSPARVMIKPGLKNFFGSFMWE"
     gene            complement(1607..2464)
                     /gene="folD"
                     /locus_tag="PK389_04250"
     CDS             complement(1607..2464)
                     /gene="folD"
                     /locus_tag="PK389_04250"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HQY22946.1"
                     /translation="MPAQILNGTLIAEKVQQHVSQRIKNYLTNGKRAPGLADILLGNA
                     PASEIYVNRKLAACQKVGIVSQHYRLNVSTSQTELIKLIDKLNNDPTIDGILIQLPLP
                     DHITTQPVLEQIRIDKDVDGFHPYNLGRLAQKKPCLRPCTPAGIMELLKSTHIDLKGM
                     DATVVGVSNIVGRPMILELLMAGCTVTAAHRFTRNLKKTVENADLLVVAAGQPGLIKG
                     DWIKHGAIVIDVGMNRMDNGQLIGDVEFEKALERASFITPVPGGVGPMTVATLLSNTL
                     YAYEWIMEK"
BASE COUNT          726 a          489 c          529 g          749 t
ORIGIN      
        1 tgttaaaagc cgtttggata tctgttttaa tgctagtttg taaactatta aaagatgttt
       61 gtttgcccca aactgaaaat tgtagctgta gagcagcctc gccaaattct agaaaagcaa
      121 gttctggagc aggggatact aaacataatg aattttgttt ggcaacttcc aaaagtattt
      181 ttttaagttt ttgcaaatcc tcattaaaac cgatatgtag cttaatgtcg catcgacgga
      241 tcggaaagcg cgttacattt tcaaactgag attttagcaa cgattcattg ggaacacgta
      301 caaaaatatt gtctttcgtg cgaattttaa ccgataacaa atcaattgaa agtacttcac
      361 ccaatgtgtc attaatacga acgaaatcac ccacttcgaa tggcttttca acaattaaaa
      421 acataccact taaaaaattc gaaattgaag tttgcgaagc aaacgcaact gcggcggtaa
      481 caattccggc tgcaattcct gcggcttcta agaaaccggt tacattgaca ccaaatgcgc
      541 gaagcggaaa tacaaacgaa agcgcaagcc caacataaaa tgtaatgcgg cgtagtaatt
      601 gagagtggtg tgctgaaaaa tgtcttgcag aaacttttcc aacgaggcgg catatataca
      661 gtgttaacag aatgctgaca acaatataac tgctgagtct taatataaaa aagaaatcga
      721 aatctttcaa agcatccata aaatgcgtaa aagtaaggtt gagatccggt agcgaaaatg
      781 gcataagatt attcccacat aaaagagcca aaaaagtttt taagacctgg ttttatcata
      841 acgcgcgccg gactgaccaa ttgcaagtcg cttaagggta atggagcgtt ttttacggtc
      901 aaggtctcga ggctgtttgt gtcttcttgg cacagatgca catcctctgt ccacagtcga
      961 ttttttttgt cgctataaag ggcgagattg tcgagaggaa tttttaattc gtgtaacaaa
     1021 tttgttcctt ttgtacgatt ttcaatcgat accggaataa tcgcatgggt agaatcttgt
     1081 ggtagatctt ctaaacgggg cgaaccgtgt atgggattat tgtaacagag ttctcctgct
     1141 cgtgtgcttt taccaaacca agtgtccgcc aaagtttctg ttgcgaattc atccagtatg
     1201 gttatggggt ttccagcact aatttgtatc caaagcggga cactgatata aacgcgcaat
     1261 gtctcaccta acggaatata acaggggcga tctaacagag aaagtaaggc acggtcgggt
     1321 agcattggtt ttaaatttat tgtgttagat gtttgtgctg caaaggtttt gaagggttgt
     1381 tcgccttcgt gataggttgt gatgtgccat ttgtggttaa agcgatcaac acaggtgaca
     1441 agctgaccaa ttttaaaaaa gcgcgattgc tcctcttcaa ttaaaaaagt tccccaccat
     1501 aattcttgtt ctgcgaggtc ttcctcatcg gttgactgaa tgggggcagc ttttgccttt
     1561 tttttaataa aaggtaaatt tttcagtttt tctaagagcc aatatttcat ttttccatta
     1621 tccattcgta tgcatacaag gtattcgata gtaacgttgc aaccgtcatg ggtccgacac
     1681 caccgggcac aggggtgata aagcttgccc gttctaaagc cttctcgaac tcaacatcac
     1741 cgattaattg tccattatcc atgcgattca tgccaacatc aatcactatc gcaccgtgtt
     1801 ttatccaatc acccttaatt agaccaggtt gcccagcggc tacaaccaac aggtcggcat
     1861 tttcaacggt cttttttaga tttcgggtaa atcgatgagc ggcagtgaca gtgcaacctg
     1921 ccatcagcaa ttctaagatc atgggtcggc ctacaatgtt agacacgcca accacggtcg
     1981 catccattcc ctttaaatcg atatgggttg attttaaaag ttccatgatc cctgcgggtg
     2041 tgcaaggccg aaggcaaggt tttttttgag ccagtcttcc taaattatag ggatgaaaac
     2101 catcgacatc tttatcaatt cggatctgtt cgagcacagg ttgcgttgta atatgatcag
     2161 gtaagggcaa ttggatcaat atgccatcta tcgtgggatc attatttagt ttgtcaataa
     2221 gctttattaa ttctgtttgt gaggttgaaa cgttgagccg ataatgttgt gaaactatcc
     2281 caactttttg gcaagcggca agtttacgat tgacgtagat ttccgacgca ggtgcatttc
     2341 caagcagaat atcagcaagt cctggtgcgc gttttccatt ggttaaataa tttttaatac
     2401 gttggctaac gtgttgctga actttttcag caatcagggt accatttaga atttgtgcgg
     2461 gcatagtctc ctcaagttct ttaagctgat gat
//
LOCUS       DAORVT010000177         2467 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_41815_length_2467_cov_2.890133, whole genome shotgun sequence.
ACCESSION   DAORVT010000177 DAORVT010000000
VERSION     DAORVT010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41815_length_2467_cov_2.890133"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="PK389_04255"
     CDS             complement(<1..724)
                     /locus_tag="PK389_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HQY22947.1"
                     /translation="MNHQLQALSTFIQDYPHLTQEEKAALLKSLKDVDKELEIAIFKL
                     DRTEKVKRTTAILLEETIDELENKRKAIEETNAALSRSLDELKAAQTQLIQSEKMASL
                     GELTAGIAHEIQNPLNFVNNFSEVSAELLDEMKDELSKGNTQDAIDMAEDIKQNLQKI
                     LIHGKRADGIVKGMLQHSRKSTGQKEPTDINALADEYLRLAYHGIRAKDNSFNATLKT
                     EFDPAIGLINVIPQDIGRVILNL"
     gene            complement(945..2084)
                     /locus_tag="PK389_04260"
     CDS             complement(945..2084)
                     /locus_tag="PK389_04260"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HQY22948.1"
                     /translation="MKAKSIKGNSPQEIKAALENAMSDGYTPTLAIIFISVKQDHKAI
                     CKLMDDAGIAVFGCTTNGEFIDEATEKGSIAILLLDINRSYFQIYFEEYPEKNYREIT
                     NRIAQKVKERFSAPAFFIAGSNMETDAEELLFGFEDVVGKEVNVYGCMAGDDFTFSDQ
                     FVFTNEYSSSRGLVCLALDEEKIMIRGVATCGWRAVGTLRTVTKSSGNHVYTVDDIPV
                     LDLTTKYGGLENVSPDNEGLLLEIAANFPLQLQREKGDPVMRPGLVIDWSDHSFFCSG
                     SVPEGSKIRFSLPPDFDVMEKVIKGVENLKATQMPEADALVVFSCAGRILSLGPLMTQ
                     EIEGIKNVWNVPMLGMFSNAELARATGGNLEMHNLTTCCVALKEK"
BASE COUNT          585 a          549 c          498 g          835 t
ORIGIN      
        1 tcagattcag gatcaccctc cctatgtctt gcggaatgac attgatcaaa ccaatagcag
       61 gatcaaattc agttttcaaa gttgcgttaa aagaattgtc ttttgcgcga atgccgtgat
      121 aggcgagacg taggtattca tctgctaatg cgttgatatc agtgggttct ttctggcctg
      181 tacttttacg gctgtgttgg agcatacctt tgacaatccc atctgcacgt tttccgtgga
      241 ttaatatttt ctgcaggttt tgtttgatat cttcagccat atcaatggcg tcctgtgtat
      301 ttccttttga cagttcgtct ttcatttcat cgagcaactc cgcactgact tctgaaaaat
      361 tattgacaaa attcaatggg ttttgtattt catgcgcaat gccggcggtg agttcaccga
      421 gggaggccat tttttcggat tgtataagtt gggtctgcgc tgctttgagc tcatcaagtg
      481 accgggatag tgcagcattg gtctcctcta tagctttacg cttattctcc agttcatcga
      541 tagtttcttc aagcagaatg gctgttgtcc gctttacttt ttcagtcctg tcgagtttga
      601 atatggcgat ttctagttct ttgtcaacat ctttaaggga tttgaggagg gctgcttttt
      661 cctcctgggt tagatgagga tagtcctgaa tgaaggtcga caaggcctga agttgatggt
      721 tcatccggaa aagatagata attgtttact gttaattgtt aaatggtaat tgttaaatgt
      781 ttaatggttt attggtttat gtgtttatgt gtttatggat ttattcttac taatcaatgc
      841 tgaagtatag catatgctcg tgcattattt gtaacaggca gctctatact cttggccctt
      901 tgctgcgcta cgctctatgc actatgctct ctgcttttcg tttttcattt ttcttttaat
      961 gccacacagc aggtcgtcag gttgtgcatc tccagattac cgccggtggc ccttgccagc
     1021 tctgcattgg agaacatgcc aagcataggc acattccaca catttttgat gccttcaatt
     1081 tcctgcgtca tcaatggtcc taatgacaga atccttccgg cgcaactaaa cactaccaat
     1141 gcatctgctt cgggcatttg tgtggctttc agattttcca cacctttaat tactttctcc
     1201 atgacatcaa agtcgggggg aagggagaag cgtatttttg aaccttccgg gacagagccg
     1261 ctacaaaaaa aggaatgatc gctccaatca atgaccagcc ccggcctcat gactggatct
     1321 cccttttcac gttggagctg caacggaaaa ttggcggcta tttccagtag cagtccttca
     1381 ttatccggcg atacattttc aagtccgcca tactttgttg taaggtcgag cacaggaata
     1441 tcatctaccg tatagacatg attgcctgaa cttttagtaa ccgttcttaa cgtacctacg
     1501 gctcgccagc cacaggtagc cactccacga atcatgatct tttcctcatc caacgcaagg
     1561 caaaccaggc cccgtgagct ggaatattca tttgtaaata caaactgatc tgaaaacgta
     1621 aaatcatcac cggccataca tccgtataca tttacctctt ttccgactac atcttcaaaa
     1681 ccaaacaaca gttcttctgc atccgtttcc atattgctgc cggcaataaa gaaggcagga
     1741 gcagaaaacc tttcttttac tttttgtgca atcctgtttg ttatttcccg gtaattcttt
     1801 tcagggtact cttcaaagta aatttgaaag tagcttctat tgatgtcaag taaaagaatg
     1861 gcaatcgaac ctttttctgt tgcttcatca ataaactctc cattggtcgt gcatccaaaa
     1921 acagcaatgc ctgcatcatc catgagtttg caaatagcct tgtgatcttg tttgacagat
     1981 ataaaaatta tggccagggt gggggtgtag ccatcagaca tcgcgttttc aagcgctgct
     2041 tttatttctt gaggagaatt tcctttaatt gattttgctt tcattttctg ttaaatggta
     2101 agtgttaaat ggtgatggtt gatgggttga taagttgatg ggttgataag ttgatgggtt
     2161 tagcttatgg cttatttggc ttatgtgctt atgtgtttat ttcgtttact cacaactcac
     2221 aactcacaac tcactttttg tttttcactc ttcacttttc actctttcgt caggggctga
     2281 acccccttcc tacttatgcg ccctttctgg gcttcctctt atcatgcttc gccttagcct
     2341 tcgccttcgc ctttttcact tttcactctt catttttcac ttaaagagac ccagcagcat
     2401 gtcgtcgagt ggaactcatg ttttccattg actgccctgc caaattcacc atacgtgtaa
     2461 aagccgg
//
LOCUS       DAORVT010000178         2406 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_43738_length_2406_cov_3.219056, whole genome shotgun sequence.
ACCESSION   DAORVT010000178 DAORVT010000000
VERSION     DAORVT010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43738_length_2406_cov_3.219056"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(365..742)
                     /locus_tag="PK389_04265"
     CDS             complement(365..742)
                     /locus_tag="PK389_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22949.1"
                     /translation="MKLKCKETVKRHFYRNEEDYSTLLKKGKEYVVLGISFSYKNRYV
                     CLETNDELPALLDIRQFEVVSNYVPSRWAVRYAREHDQLSFFPKSWVESKAFWQGLFQ
                     KEASEMFRLYQLERDLIFKEEPS"
     gene            complement(949..1149)
                     /locus_tag="PK389_04270"
     CDS             complement(949..1149)
                     /locus_tag="PK389_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HQY22950.1"
                     /translation="MGIGVIVKWAAVILIIVGALNWGLIGAFHVNAVAMLFGDASTIT
                     GIIYMLIGLAGLYKILLIATKK"
     gene            1310..2293
                     /locus_tag="PK389_04275"
     CDS             1310..2293
                     /locus_tag="PK389_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HQY22951.1"
                     /translation="MHNSFKPAWWLRNPHLQTLWAALLRPKIKLSICSERLELADGDF
                     LDFACVGNGSGPRVLILHGLNGSIDSPYARGILQAINNRGWRGIFMHFRGCSGEPNRL
                     AQSYHSGETRDLQTVITELIHREPNSPLFAIGYSLGGNVLLKWLGETGESGIENSLKA
                     AVAVSIPFELAKTAAHLKTGFSRLYQWHLLRELSASYHRKFKDLPKPFAIRNVDVTQL
                     KTFWEFDNAITAPLHGFKSADDYYEKSSSRQYLAKIKTPTLILHARNDPFTTLNALPE
                     VHEVSKHVSLELSENGGHVGFVSGKYPWKPVYWLEDRIMDYLESYSCSGNS"
BASE COUNT          743 a          535 c          413 g          715 t
ORIGIN      
        1 tttatctttt ttattgtgtt tcttttcttt tcaacagtat ccgttcattc cagttaccct
       61 attgttaaga agtcttttca ggactggaaa aaatataggc catctataca ctttactcaa
      121 taaagttcgc taacctatct acaatcatat ggttaaatgc ttacaccgtg cagggtcaat
      181 cacgtatttg ttttcaacac gctcaaagat agactggtgc gaatggattt gagaaggaaa
      241 taagggacta aactgctgca aggaagtagt aagggcagga tgcccaatct tgcttaacaa
      301 ccacaaggag tgattgttaa gcgatccaga tcggtctaca gcgcattgaa caaattaaaa
      361 cacattaact tggttcctcc ttgaatatta aatctcgctc caattgatat aacctaaaca
      421 tctcggatgc ttctttttga aaaagaccct gccagaacgc tttcgattcg acccaacttt
      481 taggaaaaaa actcagctga tcgtgttccc tcgcataccg aaccgcccat cgagaaggaa
      541 cataatttga aaccacttca aattgtcgaa tatctaacaa ggcaggcaat tcatcatttg
      601 tttctaaaca cacatagcga tttttatagc tgaaactgat acctaaaacc acatactcct
      661 ttcctttttt tagtaacgta ctatagtctt cttcgttgcg ataaaaatgc cttttcaccg
      721 tttctttgca ttttagtttc atgaataccc ccaaaaacag agtgcactta cacttctatc
      781 gtatgggaat gacataaaaa agatatccgt taaataacgg gtaagaacgc acacgaaata
      841 aaaaaactga gggtcttctt tattttaaga ccacaacact acctccttct aagatgtgtg
      901 tgttcaacac cctcagtttt tgtgcttaaa cacgtcaaac gcaatcattt actttttggt
      961 ggctattaga agaatcttat atagccctgc aagcccaatc agcatgtaaa taatacccgt
     1021 aatcgtactt gcatcaccaa ataacattgc aactgcatta acatgaaaag ctccgattaa
     1081 accccagttt agagcaccta caatgattaa aatcactgca gcccatttca caattacacc
     1141 gatacccatg acacatactc ctcttttcat aagctgaaaa catcctgtac tcattgacta
     1201 gtatatcacc taaattactt aaaaataggg cactttagcg tcgaatatac acctgttcca
     1261 ttcttcaaaa aaacttgcaa cgtgtaaatg catgctgtat tttgattaaa tgcacaactc
     1321 atttaaaccc gcttggtggc tccgaaatcc acatcttcaa accttatggg cagcgctact
     1381 acgccctaaa ataaaactgt cgatatgctc tgaacgcttg gaattagcag atggtgattt
     1441 tttagatttt gcgtgtgttg gcaatggttc tggtcctcgc gtcttaattc tacatggttt
     1501 aaacgggagt atcgattcac cttacgctag aggcatcttg caagctatca acaaccgagg
     1561 atggcgcggt atatttatgc actttcgggg ttgcagcggt gaacccaatc gactcgcaca
     1621 aagttaccat tctggagaaa cccgcgattt gcaaaccgtt attacagaat taatccatcg
     1681 cgaacccaat tcacccttat ttgcaatcgg atattcttta ggcggcaatg tcctcttaaa
     1741 atggctggga gaaaccggcg aaagtggtat cgaaaattcc ctaaaagccg ctgtggcggt
     1801 ttctatccct tttgaattag caaaaacggc agcgcattta aaaacagggt tctctcgact
     1861 ttatcaatgg cacctgctgc gtgaattatc tgcttcttat catcgtaaat ttaaagatct
     1921 gccaaaacct tttgctatcc gcaatgtcga tgtcacgcaa ctaaagactt tttgggaatt
     1981 tgataatgcc attacagcac cactgcatgg atttaaaagt gcagatgatt attatgaaaa
     2041 atcgagtagc cgacaatatc tagccaaaat caaaacacca acccttattt tgcatgcacg
     2101 taatgatccg tttacaacct taaatgcctt acccgaagta catgaagtat cgaaacacgt
     2161 atcccttgaa ttgtcagaga atggtggcca tgtcggtttt gtttcaggca aatatccctg
     2221 gaaacccgtt tactggttag aagatagaat tatggattat ttagaatcct attcttgctc
     2281 tggcaacagc tgaaccttcc ctgtgtctaa agcatataag gcgccaacga catccacttt
     2341 acctgccttg atcaattttg caatagacgg ggattgttta atatgcatga cactattata
     2401 aatatt
//
LOCUS       DAORVT010000179         2398 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_44003_length_2398_cov_3.206573, whole genome shotgun sequence.
ACCESSION   DAORVT010000179 DAORVT010000000
VERSION     DAORVT010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44003_length_2398_cov_3.206573"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PK389_04280"
     CDS             complement(<1..428)
                     /locus_tag="PK389_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22952.1"
                     /translation="MRIKTKIVVYLLPIAIVSFIIFSSCFFFFFERYISDLIYDDLDD
                     ICVSQKNILSQIIDDNKIMFEFFSENNNIIANISQYNKNNGSSDLKDLVSMMQNKLQK
                     SSVIKNITLIDLTGKIIASTDKNAFNKGHAKIPINIADTS"
     gene            complement(461..1012)
                     /locus_tag="PK389_04285"
     CDS             complement(461..1012)
                     /locus_tag="PK389_04285"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HQY22953.1"
                     /translation="MGAWFTSISIVGVRLLLAFILIVLTTLIVGGIVSHLLSKLIKST
                     KFSITDRIIGILFGLARGVFVIAIVILMVQPSIISKKEIWKSSVLVSQFEPASLWIKA
                     QLPEDLLKFYENPSKNSDDLKKTIEKATQKATGNAEDSVKDVQKPIEKPLAKPLTDIE
                     EEIGGLEKPFENIGELTDNTLSD"
     gene            complement(979..1161)
                     /locus_tag="PK389_04290"
     CDS             complement(979..1161)
                     /locus_tag="PK389_04290"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HQY22954.1"
                     /translation="MHGLNEIDYGIIGIITVSVIFGFLRGFVRESMSLMTWIVAGILG
                     TLYAMKWELGLRAFPL"
     gene            complement(1165..2031)
                     /gene="accD"
                     /locus_tag="PK389_04295"
     CDS             complement(1165..2031)
                     /gene="accD"
                     /locus_tag="PK389_04295"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HQY22955.1"
                     /translation="MSWFSKLLPSKIRTDGIKKKEMPEGLWTKCPECLAFLFRSELER
                     NLEVCPKCSFHLRLKARQRLDYFLDTAFREELATEVVADDRLKFRDTKKYKDRLLAAQ
                     KDTGEKDALIVMRGQLKGMPLVAAAFEFSFIGGSMGAAVGERFVRGVECALKNKIPLI
                     CFSCSGGARMQESLISLMQMAKTSAALGMMDKMAIPYISVMVDPCMGGVSASLAMLGD
                     INIAEPKALIGFAGPKVIEQTVREILPEGFQRSEFLLETGAIDMVVDRRHLRDTVASL
                     LAKLTHQTEKGA"
     gene            complement(2033..>2398)
                     /locus_tag="PK389_04300"
     CDS             complement(2033..>2398)
                     /locus_tag="PK389_04300"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HQY22956.1"
                     /translation="WLGEHDFSSFRGSRCQSLSPIRNIQSIKIKRLNDHVIVEFIADA
                     FLHHMVRNMMGVLFKVGRRTEPPRWAHTVLENQDRKTAGVTAPPMGLYLVEVQYPECF
                     SLPKIELGPWFLNLNMGDL"
BASE COUNT          748 a          511 c          377 g          762 t
ORIGIN      
        1 attgaagtat ccgctatatt tatcggtatt ttcgcatgtc ctttattaaa ggcatttttg
       61 tctgtagatg caatgatttt tcctgttaaa tcaataagcg ttatgttttt tattacactc
      121 gatttttgta atttattttg catcatacta acaagatctt ttaaatcact tgaaccatta
      181 tttttattat actgagatat attggctatt atattattgt tttccgagaa aaactcgaac
      241 attattttat tatcatctat aatttgtgaa agtatatttt tttgtgaaac acatatgtca
      301 tctaaatcat catagattaa atcactgatg tatctttcaa aaaagaagaa aaaacaactg
      361 ctaaaaatta taaaacttac aatcgctatt ggcagcaagt agacgacaat ttttgttttg
      421 atacgcaatt ggttattcct aattaaataa tatcgattat ttaatccgaa agtgtattat
      481 ctgtaagctc tcctatattt tcaaatggtt tttctaaacc accaatctct tcttctatgt
      541 cggtcaacgg tttcgctagc ggcttttcta ttggtttctg cacatctttt acggaatctt
      601 ctgcattacc tgttgctttt tgagtggctt tttctatggt tttcttcaga tcatcagagt
      661 ttttagatgg gttttcataa aacttcagta agtcttcagg taattgtgct ttaatccaaa
      721 gcgaagccgg ttcgaattgg gatactaata cagatgattt ccagatttct ttttttgaaa
      781 tgatagaggg ttgaaccata agaatgacaa tagcaataac gaaaacacct ctggctaatc
      841 caaataagat gccaataatc cgatcagtaa tgctaaattt agttgatttg atcaattttg
      901 aaagcaagtg actcacaatg ccacctacga tcaaagtcgt caatacaatc aaaataaatg
      961 ccaacaatag tctaacacct acaatggaaa tgctcgtaaa ccaagctccc acttcatcgc
     1021 atacaaagta cccaatatac ctgcaacaat ccaagtcatt aaagacatag attcacgcac
     1081 aaagcctctt aaaaaaccaa aaataaccga aactgtaata atcccgataa ttccgtaatc
     1141 aatttcattt aatccatgca tgatttatgc tcccttttct gtttgatgtg tcagtttggc
     1201 caaaagcgat gcaacagtat ctcgaagatg tcgacggtcg acaaccatat cgattgcacc
     1261 tgtttctaat aaaaattcac tgcgctgaaa accttccggt aaaatttcac gaactgtttg
     1321 ctcaatcact ttaggtcctg cgaaaccaat taaagccttc ggttctgcaa tattaatatc
     1381 ccctaacatc gcaagactcg cagaaacacc acccatgcag ggatcaacca tgaccgaaat
     1441 atagggtatt gccattttgt ccatcatccc caatgctgcg ctcgtttttg ccatttgcat
     1501 taatgatatg agagactcct gcatgcgtgc gccaccgctg caagaaaagc atatcaaggg
     1561 aattttattt tttaaagcgc attctacccc acgcacaaaa cgctctccta ccgcagcacc
     1621 catcgagcca ccgataaaag aaaattcaaa agcagctgca accaaaggca ttccttttag
     1681 ttgaccccgc atcacaataa gcgcatcttt ttcacctgta tctttttgtg ctgccaacaa
     1741 acgatcttta tattttttag tatcacgaaa ttttaagcga tcatccgcaa caacttctgt
     1801 cgcaagctct tctctaaagg cagtgtctaa aaaataatcg agacgctgtc tcgcctttag
     1861 tcgcaaatga aaagaacact taggacatac ttccaagttt ctttctaatt cagaacgaaa
     1921 caaaaaggct aagcactctg gacactttgt ccacaatcct tctggcatct cctttttttt
     1981 aatgccatcg gttcgaattt tcgaaggtaa taatttggaa aaccagctca ttttaaaggt
     2041 cacccatgtt gagatttaaa aaccaaggcc ctagttctat tttaggcaaa ctaaaacact
     2101 ccgggtattg tacctcaacc aaatataatc ccataggagg agcggttact ccggctgtct
     2161 ttcgatcttg attctctaat accgtgtgtg cccatcgtgg cggttcagtc ctcctcccta
     2221 ctttgaatag tacccccatc atattgcgta ccatatggtg taaaaaagca tcggctatga
     2281 attcgacgat aacatgatcg ttgagtcttt ttatcttaat agactggata ttgcgaatag
     2341 gagaaagact ttggcatcga gatccgcgaa aagatgaaaa atcatgttcc cctagcca
//
LOCUS       DAORVT010000180         2397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_44028_length_2397_cov_3.895389, whole genome shotgun sequence.
ACCESSION   DAORVT010000180 DAORVT010000000
VERSION     DAORVT010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44028_length_2397_cov_3.895389"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PK389_04305"
     CDS             <1..378
                     /locus_tag="PK389_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22957.1"
                     /translation="VKPSHVKRYLTLKGCINKPLKQISINKASEQQDFAIKKGRVSEA
                     AWYLEKKREAETGITKEMQNEHEALYRKLQSYLGFGQITQFNTELTKITKEMELIDAF
                     RFEVEKIKEKLIELSSLVQVSAG"
     gene            691..1140
                     /locus_tag="PK389_04310"
     CDS             691..1140
                     /locus_tag="PK389_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22958.1"
                     /translation="MKIFNLTIFLVGASLLLSGCVTSRAATASIAKQSMVGMSKESVL
                     SCMGVPVDKMTEGSTEVWVYGSGGGTDSIGSAFGQTQGSAHVIGNSVYGSSSGSAIGF
                     GRSRSRYCTINMVFQKGRVSAVNYSGRTGGLATQGEQCAYAIENCVH"
     gene            1152..1397
                     /locus_tag="PK389_04315"
     CDS             1152..1397
                     /locus_tag="PK389_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22959.1"
                     /translation="MKKHLLRLVPFVLFFLGNFLVFYTFEAFPVTCIEAQFARGRQGW
                     NCSWNDGAALQIAGGLTIVLGAYFSFKKINKISIFDY"
     gene            1512..>2397
                     /locus_tag="PK389_04320"
     CDS             1512..>2397
                     /locus_tag="PK389_04320"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQY22960.1"
                     /translation="MHNFRSLTLSEWRQFRSIDIDLSKHITILTGQNGCGKTTILNVL
                     GHHFGWNVHFVSTPYLAKRQAKTLWSDIFGFNSRSFNEDDTQTREVGTIIYDNNAICR
                     LMVNPQVSAQYKPAYAGAQEVIGLHVPSHRPASTYHNVEEIPTNPATALQFYQNYQNL
                     LNQLYASGNSKNPGRIQKQSLISLAAFGEGNNHMQSNPEFIKIFEGFQNVLRHILPPE
                     IGFQSLQIRMPEVVLVTSSGDFALEAMSGGINSLFGMAWQIYMFGFDKDKCTVTIDEP
                     ENHLHPSMQRSFLPSLAKA"
BASE COUNT          688 a          467 c          550 g          692 t
ORIGIN      
        1 gtgaaacctt cacatgttaa gagataccta actcttaaag gttgcataaa caaaccatta
       61 aaacagattt caataaacaa agcttccgaa cagcaagatt ttgctatcaa gaaggggcgt
      121 gtttcagagg cagcctggta tcttgagaag aagcgtgagg cagagactgg gataactaaa
      181 gaaatgcaga atgagcatga agctctttat agaaagttac agagctatct gggatttggg
      241 cagataaccc agtttaatac tgaactaacg aagataacaa aagagatgga attgatagat
      301 gcgttccgct ttgaagtaga aaaaataaag gaaaaactta tagagctttc atccctggtg
      361 caggtttctg cgggctagtg aaaacctaac aaaaccccac attcaatatt agcttttgtc
      421 aacttttcac tcaatatatt ggtttacaaa gctgtcctat aacatctaca agtgtattgg
      481 gtggtcttta aggcgacccg ggggcgttag aacctcgtaa caagcggatg gcgtgtccgt
      541 catcacgtcg cttctcggtc tatgaccggg aggcgatggc gtatgtccaa gcttcggtta
      601 aaggccttcg cggtcgtctt gttacgactt ctaaactccc ggtcaccaga gaggacactc
      661 tggtggcttt ttcttaaccg ggagatgaac atgaaaattt ttaatctaac aatatttcta
      721 gtgggagctt ctctgctttt gagcggatgc gttaccagtc gtgctgctac ggcatctatt
      781 gcgaagcaga gcatggttgg catgagcaag gaaagcgtct tgtcttgtat gggggtgcca
      841 gttgacaaga tgacagaagg ctcgaccgag gtgtgggtgt acgggtcagg tggtggaaca
      901 gacagcattg gaagtgcttt cggccagaca caaggatcgg cccatgttat cggcaactca
      961 gtttacggct cgtccagtgg ctcagccata ggttttggtc gtagcagatc acgttactgc
     1021 actatcaaca tggtgtttca aaaaggtcgc gtctccgcag tcaattattc agggcggacc
     1081 ggcggcttgg cgacgcaggg tgagcaatgt gcctatgcca ttgaaaactg tgtccattag
     1141 ggggacttac catgaagaaa catttgttgc ggttggtgcc atttgtgctc ttttttttag
     1201 gaaacttcct agtattttac acttttgaag cttttccagt cacttgcatc gaagctcaat
     1261 tcgcaagagg caggcagggc tggaattgca gttggaatga cggcgcagca ttgcagattg
     1321 ctgggggtct gactatagtt ttaggtgctt atttctcttt taaaaaaatt aataagatca
     1381 gtatttttga ttactaatgc attcattaga agagtcttat gaatacagat tacctcacta
     1441 acgctgctgt cctctcaaat ttaaattttt tgtatgttta tttagcaagc agaattggtt
     1501 aggtattgta tatgcacaat tttagatctc ttacgctgtc tgaatggaga cagtttagaa
     1561 gtattgatat tgatctctct aagcacatca caattttgac gggtcaaaat ggatgtggga
     1621 agacgactat tctaaatgtt ctgggccatc attttggctg gaacgtccat tttgtttcca
     1681 cgccgtatct tgcgaaaaga caagcaaaaa cgctttggtc agatattttt ggattcaata
     1741 gcagatcttt taatgaagat gatactcaaa ccagagaagt aggtacgatc atttacgaca
     1801 acaatgccat atgcaggctt atggtcaatc cgcaagtaag tgctcaatac aaacctgctt
     1861 atgcaggcgc acaagaagtt atcggtttgc atgttccctc tcaccgtccg gcgagcacgt
     1921 atcataatgt cgaagaaata cccaccaatc cagcaactgc tttgcaattt tatcaaaatt
     1981 atcaaaattt acttaaccaa ttatatgcct caggaaattc taaaaatcct ggaaggatac
     2041 agaagcaatc tcttatttct ttggcggcat ttggtgaagg taacaaccat atgcaaagta
     2101 atcccgaatt tataaaaatc tttgaaggct tccaaaatgt attgcgtcat atattgcccc
     2161 cagaaatagg ctttcaaagt ttacaaatta gaatgcctga agttgttttg gtaaccagtt
     2221 cgggcgattt tgctttggag gcaatgtccg gaggtattaa cagccttttt ggtatggcat
     2281 ggcaaattta tatgttcggc ttcgacaagg ataaatgcac tgtcacaata gatgaacctg
     2341 aaaatcatct acatcctagt atgcagcgat cgtttttgcc atcactggct aaggcct
//
LOCUS       DAORVT010000181         2393 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_44183_length_2393_cov_3.478614, whole genome shotgun sequence.
ACCESSION   DAORVT010000181 DAORVT010000000
VERSION     DAORVT010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44183_length_2393_cov_3.478614"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PK389_04325"
     CDS             complement(<1..423)
                     /locus_tag="PK389_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HQY22961.1"
                     /translation="MKYIDEFRTGTVAQKIAAKIASEASSRRDYHFMEFCGGHTHAIS
                     RFGIIDLLPNNVHMIHGPGCPVCVLPIGRVENAIRLAQTPGVILCSYGDMLRIPTASG
                     MSLLKAKAQGADVRMVYSSVEAIKIAQKNPQHQVVFFAI"
     gene            complement(447..680)
                     /locus_tag="PK389_04330"
     CDS             complement(447..680)
                     /locus_tag="PK389_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011394072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HQY22962.1"
                     /translation="MCLAIPACVELLINENYAIVNLGGIRKEISLALVEDITPGDYVI
                     VHVGFALQKLDQMEAERTLAMFAEISALNKENT"
     gene            755..1330
                     /locus_tag="PK389_04335"
     CDS             755..1330
                     /locus_tag="PK389_04335"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HQY22963.1"
                     /translation="MTKQNSFTLYLVILLIGLLSLSVLPNSVWADNGVWIDVDTSAHT
                     LSVMQGDTIQAVFKNVAIGRYGTTWSKMTRDDKTPLGKFRVGWINEKSRYYRFFGLNY
                     PNLDTAKRALDENRITEETWLSILKAKIMGKTPPQDTPLGGHIGIHGIGRGDQDIHHS
                     YNWTNGCVALTNEQIDQLGKWIKPGVLVNIR"
     gene            1560..1886
                     /locus_tag="PK389_04340"
     CDS             1560..1886
                     /locus_tag="PK389_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpp/OprI family alanine-zipper lipoprotein"
                     /protein_id="HQY22964.1"
                     /translation="MHIERINSEEIGMKEKIKHPVRMLTLAAIAGAFIGLTGCATTGQ
                     LEELQNKVNADIAAVKAEAAAAKAEAQAASGKANDAVRIADEANRRSMDTESKIDRMF
                     KKAMHK"
     gene            complement(1971..>2393)
                     /locus_tag="PK389_04345"
     CDS             complement(1971..>2393)
                     /locus_tag="PK389_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HQY22965.1"
                     /translation="SIGSGSYLIHGTIKPFGVGMRVSAGCVRMYPEDIEALFDKVPVG
                     TQIQIVNQPIKLGWLLDSLFIELHPPLEEDEGKYTNYKQIVVAAINDFLELKSSKKNI
                     PADFEIDQETLKQAITEKNGMPVLISRSQIQLRAQNNN"
BASE COUNT          726 a          430 c          514 g          723 t
ORIGIN      
        1 aatggcaaaa aataccactt gatgttgcgg atttttctgt gcaattttta ttgcttctac
       61 actggaatat accatgcgta catctgcacc ctgtgctttt gctttcaaca aactcatgcc
      121 gcttgcagtt ggtatgcgta acatgtcgcc gtaactacag agtatcaccc cgggtgtttg
      181 tgccaaccga atagcatttt ccacgcggcc gattggtaaa acacataccg ggcagccggg
      241 gccatgaatc atatgtacat tgttaggaag cagatcaata ataccgaaac gtgaaatggc
      301 atgagtatgt ccgccgcaaa attccataaa atgataatcg cgtcgagaac ttgcttcgga
      361 agcgattttg gcagcgatct tctgcgccac agtacctgta cgaaattcat caatatattt
      421 cataatggat cattcaatct cgatcattag gtattttctt tgttgagagc tgatatttct
      481 gcaaacatgg ctagtgtgcg ttctgcttcc atctgatcca gtttctgtag cgcgaaacca
      541 acgtgtacga tgacataatc cccaggagta atatcctcaa ccaatgccaa ggaaatttcc
      601 ttgcgtatgc cacctagatt gacgattgca taattttcat taatcaatag ttcgacacag
      661 gcaggaatag caaggcacat aagcatttta tgtaagtttc taaatgctta aattatgaca
      721 taattaacta tctcaatttt taatagtcta gaaagtgacc aaacaaaatt cttttactct
      781 ttatttagtg attttactga ttggattatt gagcctgtca gtattaccga attctgtttg
      841 ggcagacaat ggagtttgga ttgatgtaga tacatctgca catactttgt cagtgatgca
      901 gggcgatact atccaagcgg tttttaagaa tgtggctatt gggcgctatg gcaccacctg
      961 gtccaagatg acaagagacg acaaaacccc gttgggaaaa ttcagggtgg gctggatcaa
     1021 tgaaaaaagc cgctattatc gtttttttgg gctgaactat ccaaatcttg atacagcaaa
     1081 acgtgcgctg gatgaaaaca ggattactga agaaacctgg ctatcgatat tgaaagcgaa
     1141 gattatggga aaaacgccgc ctcaggatac gcctcttgga ggtcatatag gcatacatgg
     1201 aattggcaga ggcgatcaag acattcatca cagttataat tggacaaatg gttgtgttgc
     1261 actaacaaac gagcagattg atcaactggg taaatggatt aagccgggtg ttttagttaa
     1321 tattcgttag aatccccagt cggttgatta gcaatgtctt gtgttgttaa atgcttgaat
     1381 tgatattaaa agaaaaaata tgcattttta tcagatacct agaaagtaat ttgttattca
     1441 atgatggggt tttttataaa ttagttgcat aatccattta ttttgtagaa aaaatcagat
     1501 aagattccag cgggttgctg atactcaggt attagtaact agtaaagtgg tggatattta
     1561 tgcatattga aagaataaat tctgaggaga ttggaatgaa agaaaaaatc aagcatccag
     1621 ttcgtatgtt aacactggca gcaatagcag gtgcttttat tgggctaacg ggctgtgcaa
     1681 ccacaggtca attagaagaa cttcagaaca aagttaacgc tgatatagct gcggttaaag
     1741 ccgaagcagc agctgctaag gcggaagctc aagctgctag cggaaaagca aatgacgcag
     1801 tacgtatagc agatgaagca aacagacgtt caatggatac agaatcaaaa attgatcgta
     1861 tgtttaagaa agctatgcat aaataataac aaagtaaata tagtagtaag ttattatatt
     1921 cataaaaaaa cccctcagtt gaggggtttt tttatgaatt ttttttggaa ctaattgtta
     1981 ttttgtgcac gtagttgaat ttgagagcgt gatattagaa cgggcattcc gtttttttcc
     2041 gttatcgctt gttttagggt ttcttggtct atttcaaaat ctgcaggaat atttttcttg
     2101 ctgcttttta gttcaagaaa atcattaatt gcagctacga ctatttgttt atagttggtg
     2161 tatttcccct catcttcttc cagcggtgga tgaagttcta taaaaagcga gtcaagtaac
     2221 catccgagct taatgggttg gttgacaatt tgtatttgcg tacccaccgg aactttatcg
     2281 aagagcgctt cgatgtcttc tggatacatg cgaacacacc cagctgaaac gcgcattcca
     2341 accccgaatg gtttgatagt gccatgtatg aggtagctgc cactgccgat gct
//
LOCUS       DAORVT010000182         2391 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_44275_length_2391_cov_2.397688, whole genome shotgun sequence.
ACCESSION   DAORVT010000182 DAORVT010000000
VERSION     DAORVT010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44275_length_2391_cov_2.397688"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PK389_04350"
     CDS             complement(<1..405)
                     /locus_tag="PK389_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005002550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HQY22966.1"
                     /translation="MNTIPATIDLNPSGGDPEASVIWMHGLGADGNDFVPIVERFKSL
                     ESKVRFVFPHAPVRSITVNGGMRMRAWYDIVGLNISYKEDSEGIKDSARLIELLIKRE
                     QELGIASNQIVLAGFSQGGAMALYAGLRYPEPL"
     gene            656..1147
                     /locus_tag="PK389_04355"
     CDS             656..1147
                     /locus_tag="PK389_04355"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HQY22967.1"
                     /translation="MTKLFKRIFQILIALITIFVIAIIVIVNFVNPNQFKDILEKEVL
                     AKTGFVLAVQGPIQWRWFPMLSLEFQDISIQNLAPFSGQLLSAKAIHAECELLPVFLG
                     KIALAINVKGLDLALSRNAKGQTNWENLNQHLKEQETNKTTTIESNPATKIQTQPRLC
                     SSL"
     gene            1255..>2391
                     /locus_tag="PK389_04360"
     CDS             1255..>2391
                     /locus_tag="PK389_04360"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HQY22968.1"
                     /translation="MSNDFTPFFLNCELTSNHQTLAHIFFNTEWILKKEKEQLNLQKI
                     TLKFQLPDGITNLITGDTLIEGFSQKPIIKANLLGNALQFGTVKIAQIKTMMTAQENI
                     VTFAPIDIHIAKSQQNATLKLDMRGDTPKIYITHEAHDFEINDLLTLFNKKDKLSGKT
                     RITMNLSATGTDLQALQNSLSGKAAIEINSGKFHGIDLMNLLKSTQSSIHSLIDALTH
                     KLSIDSLSVIKSELAKWKTESNSSAFTPFNSLKATATITNGVIQNPDLAINQTDYAIN
                     GAGTINPQANNIQYQTTLQLKNNPYPANDKAGTFLFQTPLHIQIQGTLDDPKVKPELS
                     RYTNSALEFAQKELVKKVIDKTVEKTLDKIKGNSAVQDTLQKALG"
BASE COUNT          837 a          555 c          387 g          612 t
ORIGIN      
        1 taaaggctct ggataacgca gtcccgcata caatgccatc gcgcctcctt gcgagaagcc
       61 ggctaaaaca atctggttac tcgcaatacc caattcttgc tcacgcttaa tcagtaattc
      121 aattaaccgc gccgaatctt ttataccttc actatcttct ttgtatgata tattcaatcc
      181 aacaatatcg taccaagcac gcatccgcat cccgccattc accgttatag atcgcaccgg
      241 agcatgtgga aatacaaatc gtactttgga ttccaatgat ttaaatcgct ccacaatggg
      301 cacaaaatca ttaccatcgg cacccagccc atgcatccaa ataacagaag cctctggatc
      361 tccacccgaa gggttgagat caatggttgc aggaatagta ttcaaagggc atcactcaca
      421 ataatttaag ttcttttata gtgccaggaa ataccaaaaa tgtctaaaca ttgacaaaca
      481 tcccgcatta aaaaaagtcg tttgttgcct aacctattac agcaagctca ttctaatcaa
      541 attaattgct cagcatatcc gctatgtaat tcccctgtag cccgaaatca tggcctttgc
      601 gctaaactac aattcctata taatgggcaa gagaaataac gggagatcta gaaccatgac
      661 gaaactcttt aaacgcatct ttcaaatact gatagcatta atcactatct ttgttatcgc
      721 tattattgtg atcgtcaact tcgttaaccc caatcaattt aaagacatcc tcgaaaaaga
      781 agttcttgca aaaacaggat ttgttttagc cgttcaaggg cccattcaat ggcgatggtt
      841 tcccatgttg tctttggagt ttcaggatat cagcatccaa aatcttgccc ccttttctgg
      901 ccaactgctt tctgcaaaag ctattcacgc tgaatgtgaa ctcctacccg tttttttagg
      961 caaaattgca ctcgctatta atgtaaaagg tttagatctc gcactttctc gaaatgcaaa
     1021 agggcaaacc aattgggaaa acctgaatca acacttaaag gaacaagaaa caaataaaac
     1081 tacaaccata gaatccaatc ctgcgactaa aatacaaacc caacctcgtc tctgttcatc
     1141 actttaaaca gcgtaaaaat tcaagggggg acaattgctt ttactgatcg ctcaaaaaac
     1201 agcgaatatg ctctccacca tgtcaattta tctgcagata atttattcaa aggtttgagc
     1261 aatgatttca ccccattttt tttaaattgc gaattgacct ccaatcacca aacactcgct
     1321 cacatttttt ttaacacgga gtggatatta aaaaaagaaa aagaacagct caacctgcaa
     1381 aaaattacac tgaaatttca actacccgat ggcattacaa atctgattac cggagatacc
     1441 ttaatagaag gattctcaca aaaaccgatt atcaaagcga atttactcgg caatgcgctt
     1501 caatttggca cagtaaaaat tgcacaaata aaaacaatga tgacagcgca agaaaacatc
     1561 gttacttttg cacctatcga tattcatatt gccaaaagcc aacaaaacgc aacgctaaaa
     1621 ttggatatgc gaggagacac gcctaaaatt tatatcaccc atgaagcaca tgatttcgaa
     1681 ataaatgact tgctcacgct ctttaataaa aaagacaaac tgtcgggcaa aacacgtatt
     1741 acaatgaact tatcggcaac aggaacagat ctgcaagcat tgcaaaatag tctttccggg
     1801 aaagcggcca ttgagattaa cagtggcaaa tttcatggca ttgatttaat gaatctttta
     1861 aaaagtaccc aatcaagtat ccatagtttg atcgacgcac taacgcacaa actcagcatc
     1921 gactcgctgt ctgttattaa atccgaattg gcaaaatgga aaacagagtc taactcgagt
     1981 gcgtttacgc cctttaattc actcaaagca accgcaacta tcaccaatgg cgtgatacaa
     2041 aatcccgatc ttgctattaa tcagaccgat tatgccatca acggggcggg gaccattaac
     2101 ccacaagcaa acaacataca atatcaaaca actttacaac tcaaaaataa tccttaccca
     2161 gcaaatgata aagcaggcac tttcttgttt caaaccccct tgcacattca gatccaaggt
     2221 acgctagatg atcccaaagt aaaaccagaa ctctcaagat acacaaatag cgcattggaa
     2281 tttgcacaaa aagagttggt taaaaaagtg atcgataaaa cggtcgaaaa aacacttgat
     2341 aaaataaaag gaaacagcgc agtgcaggat acgctacaga aggccttggg a
//
LOCUS       DAORVT010000183         2387 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_44414_length_2387_cov_3.469125, whole genome shotgun sequence.
ACCESSION   DAORVT010000183 DAORVT010000000
VERSION     DAORVT010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44414_length_2387_cov_3.469125"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PK389_04365"
     CDS             complement(<1..653)
                     /locus_tag="PK389_04365"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HQY22969.1"
                     /translation="MDKAAIVQKVLKSTSKEPQNRTGTFFAPSNIALCKYWGKRNAEL
                     NLPVTASLSVSLGHYGAKTTLSFAEAGFQDDIVVNGKQMNINSDSAFVLNLTAYLNLF
                     RPTPTTRFCVETETNVPIGAGLASSACGYAALIGAMDQLYGWQLPLQTLSLLARLGSG
                     SASRSFWQGFVEWQTGTAEDGIDSVGVSLPQMWPDLRIGLLILNPASKRISSRKAME"
     gene            complement(646..1638)
                     /gene="mvk"
                     /locus_tag="PK389_04370"
     CDS             complement(646..1638)
                     /gene="mvk"
                     /locus_tag="PK389_04370"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HQY22970.1"
                     /translation="MALKSFSASAPGTLMLLGEHAVLNNKPAIVLAIDKRITVTLHVS
                     HASISNAPKILITSNLGRIECNLNEIKNLSNQKPFQFVFVAIKFFKKELKIRNQGFEL
                     IIDSEFSPNCGLGSSAAVTVALVAVLLQFLSKSTEMLFEISREIIREVQGVGSGADVA
                     ASVYGGVIVYQQQSPYIIKKLDCVLPLVVIYSGSKTPTPVVIKKVEMLQNKYPDVINT
                     LYDAMEHAVNRASVFIENKDWAGLGEVMNIQQGVMNALGVGTPVLNQLIDSLTSTPGI
                     YGAKISGSGLGDCVIAIGSNEYVGADRGNRPFEQQENGIQKIPLSVSLQGVHYG"
     gene            complement(1619..>2387)
                     /locus_tag="PK389_04375"
     CDS             complement(1619..>2387)
                     /locus_tag="PK389_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HQY22971.1"
                     /translation="RCSIRDVLKTPFELLQYTVTHLLETLNVTLSDGLEIRAASEIPI
                     GCGMGSSAAIVMSTLYALAHFLKLEIDPARFLALGREAENLQHGYSSGLDLHLAMKGG
                     CLRFEEGQTFVRTLPNMPLCIVQTGTPESTTGQCVSRVSKHFTQGAMADDFAAITELF
                     DQALQANNIPNMQACIRQNHRLLVKIGVVPPKVQDFICALEKEGAAAKICGAGAVKGN
                     NAGVVLVLSDRDLSSVTRDYGYSLQSVHGDLYGTQIV"
BASE COUNT          709 a          573 c          437 g          668 t
ORIGIN      
        1 atttccatgg cctttcttga acttatccgt ttggatgctg gattcagtat taataaaccg
       61 attcgcaagt caggccacat ttggggcaaa gaaacaccta cagaatctat cccatcttct
      121 gccgttcctg tttgccattc tacaaatccc tgccaaaacg atcgactggc actcccgctt
      181 cctaaacgcg ccagcaaaga aagcgtttga agcggcaact gccaaccata caattgatcc
      241 attgcgccta ttagtgcagc ataaccgcaa gctgaagacg ccagccctgc gccaatcggc
      301 acattcgtct ctgtttcaac acaaaaacgt gtcgtcggtg tgggtctaaa taaatttaaa
      361 tacgctgtta aatttaaaac aaaagcggaa tcactgttaa tattcatctg cttgccattg
      421 acaacaatat catcctgaaa tcctgcttca gcaaaagaaa gcgttgtttt agcaccataa
      481 tgcccaagtg aaacagagag acttgcggtt acaggtaaat tgagttcggc atttcgtttt
      541 ccccagtatt tacataacgc aatattagat ggcgcaaaaa acgtgcccgt tctgttttga
      601 ggttcttttg aagttgattt tagaactttc tgaacaattg ctgccttatc cataatgcac
      661 accttgcaaa gaaacagaca ggggaatttt ttggatgccg ttctcttgct gttcaaatgg
      721 gcgattacct cggtcggccc ctacatactc gttagaacct atagcgataa cacaatcacc
      781 cagccccgaa cccgaaattt tagcgccata aatacccggg gtgctggtga ggctatcgat
      841 taattggttt aacacgggcg ttccaacccc gagtgcattc atcactcctt gttgaatatt
      901 catcacctca cccaagcctg cccaatcttt attttcaata aagacagaag cccggtttac
      961 cgcatgttcc atggcatcat acaatgtgtt aatcacatcc ggatatttat tctgtagcat
     1021 ttctactttt ttaatgacga caggtgttgg cgtttttgag ccagaatata tcacaaccaa
     1081 gggcaacaca cagtctaatt tttttataat atacggtgat tgctgttggt aaacaataac
     1141 gccaccataa acactggctg caacatccgc acccgaaccc accccttgca cttcgcgaat
     1201 aatttctctc gatatctcaa aaagcatttc tgtgcttttt gaaaggaatt gcaataaaac
     1261 agcgaccaaa gcgactgtta cagctgccga tgagcctaat ccgcaattag gcgaaaattc
     1321 tgaatcaatg attaattcaa aaccctgatt gcgtattttt aattcttttt taaaaaattt
     1381 aatagctaca aatacaaact gaaagggttt ttgatttgaa aggtttttta tttcattcag
     1441 attgcattct attctaccca gattagacgt tatcagaatt tttggcgcat tactaattga
     1501 cgcatggcta acatgtaatg taactgttat gcgtttatcg attgctaaca caatagcggg
     1561 tttattattc aagacagcat gttcgcccaa cagcatcaaa gttcccggtg ccgaggcact
     1621 aaacgatttg agtgccatat aagtccccat gaacagattg aagactataa ccataatctc
     1681 tcgtaacaga agacaaatct cgatcagaaa gcactaaaac aacccctgca ttgttaccct
     1741 ttacagcgcc ggccccacaa attttggctg cagcaccttc tttctcgagt gcgcaaataa
     1801 agtcttgcac tttcgggggt acaaccccaa ttttcactaa aagtcgatgg ttttgccgaa
     1861 tgcacgcttg catattggga atgttatttg cttgcagcgc ttgatcaaaa agctctgtaa
     1921 tagcagcaaa atcgtccgcc attgctccct gtgtaaagtg ttttgaaacc ctgctaacgc
     1981 attgccctgt tgtggattca ggcgtacccg tttgaacaat acacagtggc atatttggca
     2041 aagtgcgaac gaatgtctga ccttcttcaa atcgcaaaca cccccccttc attgctaaat
     2101 gtaaatctaa tccactggaa taaccatgtt gtaaattttc ggcttcacga cccagcgcca
     2161 aaaatcgcgc gggatcaatc tctaatttca aaaaatgtgc gagcgcgtat aaggtactca
     2221 tcacgattgc agcagaagat cccataccac aaccaatggg aatttcagat gcagcgcgaa
     2281 tttctaagcc atcagacaac gtaacattta aggtctccaa caaatgtgta accgtatact
     2341 gcaataactc aaaaggggtc tttaacacat ctcgaatact acaacgc
//
LOCUS       DAORVT010000184         2384 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_44507_length_2384_cov_3.886647, whole genome shotgun sequence.
ACCESSION   DAORVT010000184 DAORVT010000000
VERSION     DAORVT010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44507_length_2384_cov_3.886647"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..320)
                     /gene="atpE"
                     /locus_tag="PK389_04380"
     CDS             complement(45..320)
                     /gene="atpE"
                     /locus_tag="PK389_04380"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HQY22972.1"
                     /translation="MANLQGITTIAVAILISIGAIGTAIGFALLGGKFLEGSARQPEM
                     MGQLQVKMFIIAGLLDAVTMIGVGVALFFTFANPFMSQLLDLLQAVK"
     gene            complement(381..1154)
                     /gene="atpB"
                     /locus_tag="PK389_04385"
     CDS             complement(381..1154)
                     /gene="atpB"
                     /locus_tag="PK389_04385"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005319615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HQY22973.1"
                     /translation="MSESLGEALNATSYIQHHLQHWTLKTEFGTYYLDTLLSSWVIGI
                     TFLFLFWRVAKRATVDAPGFMQNFIELLYEFVQKQVQETFHGRSVLIAPLALTIFVWV
                     FLMNFMDLIPVDLLPRVLANVGVPHFKSVPTADPNLTLGMSLSVFLLTIVYAIKIKGF
                     GGFLKEITCKPFGCWLFPVNIALKLVEELAKPLSLGLRLFGNMYAGELIFILIGLLGF
                     SQGFLGVPWAIFHILVITIQAFIFMMLTIVYLSLASESH"
     gene            complement(1310..1672)
                     /locus_tag="PK389_04390"
     CDS             complement(1310..1672)
                     /locus_tag="PK389_04390"
                     /inference="COORDINATES: protein motif:HMM:NF015834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HQY22974.1"
                     /translation="MVSVVFQRLGWVILFIACISVALLYLFSLNKGVSFALGAGVSLF
                     PILLFGRIFFKTKDTASTAKKIVNAFYIGEAIKILTTIVLFILVFQWQALVPLFLFLG
                     FIAAQLSCLLIFSLKCNN"
     gene            complement(1674..2075)
                     /locus_tag="PK389_04395"
     CDS             complement(1674..2075)
                     /locus_tag="PK389_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011747081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY22975.1"
                     /translation="MLDHVSFAVKNYEKSCKFYDETLLILGYKREVTLEFPGGRVAGY
                     GNGGVRPCLWIADGGREDETIGTAQGLHFAFVAPSAAAVDQWYAACLKLGGKDNGAPG
                     TRPHYHPNYYGAFVIDPNGWRIEACFHHHQP"
     gene            complement(2167..>2384)
                     /locus_tag="PK389_04400"
     CDS             complement(2167..>2384)
                     /locus_tag="PK389_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22976.1"
                     /translation="YEQIKFKQRKSVLRLGGGVQKMFSDSFGIRTTVVWENTSRFNNI
                     TQNYTSRTYTAKLKDSWTYGIGAFVMF"
BASE COUNT          788 a          541 c          429 g          626 t
ORIGIN      
        1 tgcccgatta atgttgcgtt aatatccatg tacgatagcg ccctttattt aaccgcttgc
       61 aaaagatcta acagctgaga cataaacgga tttgcaaaag taaaaaacag cgcaacaccc
      121 acgccaatca tcgttaccgc atctaagagg cctgcgataa tgaacatttt cacttgcaac
      181 tgccccatca tttcgggttg tcttgcagat ccttccaaga actttccgcc cagtaatgca
      241 aagcctatgg cggttccaat ggcgccaata ctaattaaaa tagccaccgc aatagttgta
      301 atcccttgca gattagccac caatgtaaca gcttgaatag acatttccga cataataccc
      361 ccaatcaaaa taaaaacaaa ttaatgcgat tcactggcta ggctaagata cacaatggtt
      421 agcatcataa aaataaacgc ttgtatggta atcaccaaaa tatgaaaaat agcccaagga
      481 acccctaaaa aaccttgtga aaaccctaat aatccaatca aaataaaaat caattcaccc
      541 gcatacatgt tgccaaaaag tcgtaaccct agcgatagcg gttttgccaa ttcttccacg
      601 agctttaacg cgatattcac gggaaacaac cagcacccaa agggtttgca ggttatttct
      661 tttaaaaacc cgccgaatcc tttaattttg atagcataaa caatcgtcaa taaaaagact
      721 gatagcgaca ttcccagcgt taaattggga tctgcggtag gaacagattt aaagtgcggc
      781 acccctacat ttgctaacac tctgggaaga agatcgactg ggatcaaatc catgaaattc
      841 atgagaaaca cccagacaaa aatcgttaat gccaaaggcg ctatcaacac gcttcttcca
      901 tgaaaagttt cttgaacctg cttttgcaca aactcgtaga gcaactctat aaaattttgc
      961 ataaaaccag gagcgtctac ggtcgcacgt tttgcaacac gccaaaacag aaaaagaaaa
     1021 gttataccaa taacccaaga agaaagcagt gtatcaagat aatacgtccc aaactctgtt
     1081 ttcagtgtcc aatgttgcaa atgatgctga atgtagctcg tcgcatttaa agcttcgccc
     1141 aaactctcgc tcacaacatt tgtaccttct gttatttaat ctccaccttc tttgaaatat
     1201 agcagttgtt tataagttaa gacaagcaac actgcggtaa catattttta gcagtaggtt
     1261 atcaggtcct ctagattgac tgcgcgttag tttgttaggt gtcatgcatt caattattac
     1321 acttcaatga gaatatcaac aagcaactca attgcgctgc aataaatcct aaaaataaaa
     1381 ataaggggac taatgcttgc cattgaaaca caagtataaa taatacaatc gtggttaata
     1441 ttttgatcgc ttcgccaatg taaaaagcat tgacaatttt tttagcggtg cttgctgtat
     1501 ccttagtttt aaaaaatatc cgaccaaaga gaagtatagg gaaaaggctt acaccagcgc
     1561 ctagcgcaaa agaaacgccc ttgttaagac taaacagata tagcagggcg acgctaatgc
     1621 aggctatgaa taaaataacc cagccaaggc gttgaaaaac aacagaaacc atattatggc
     1681 tgatgatggt gaaagcaggc ttcaatacgc caaccgtttg gatctataac aaacgcaccg
     1741 taataatttg ggtggtagtg cggacgtgtt ccaggggcac cattatcttt gccacccagt
     1801 tttaaacaag cggcgtacca ttgatcaaca gctgctgcac tcggcgctac aaaagcaaaa
     1861 tgcaagcctt gcgccgttcc aatggtttcg tcttcgcgtc caccatcggc aatccacaag
     1921 caggggcgga caccgccatt gccatatcca gccacacgcc cacctggaaa ctcaagggtc
     1981 acttctcttt tatatcctaa gattaataag gtttcgtcgt aaaacttgca gcttttttca
     2041 tagtttttga cagcaaaaga aacatgatcg agcatttgct acccctctat atctgtccat
     2101 gaatatagca tcgtagcgca attgtattca gcaatacaat ggtcaagcaa aaactgtgat
     2161 gctttgttaa aacataacga aggcgccaat gccataagtc caggaatctt taagtttggc
     2221 tgtataggtt cgacttgtgt agttttgagt aatattatta aaccgggatg tattttccca
     2281 aactacagta gtacggatac caaacgagtc cgaaaacatt ttttgcacac cgcccccaag
     2341 gcgtaataca ctttttcttt gcttaaattt tatttgctca tatc
//
LOCUS       DAORVT010000185         2376 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_44779_length_2376_cov_3.531237, whole genome shotgun sequence.
ACCESSION   DAORVT010000185 DAORVT010000000
VERSION     DAORVT010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44779_length_2376_cov_3.531237"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="PK389_04405"
     CDS             complement(<1..1226)
                     /locus_tag="PK389_04405"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HQY22977.1"
                     /translation="MISIGLMSGTSMDGIEAALLETDGSPTVLRALAHISLTYDPRFK
                     ILLKSAEYAVKKCAGNLEQAKIYYPQAIPDYIKEAFSINTNLDIEQKISDLSFYLYQE
                     NRSNSQITLDKIIEHSTKLHGIAVKKLLNAQKMSARQVDVVGYHGQTLFHNPERKICI
                     IVGNGQQLADEIGITVVNDFRSRDIEAGGCGAPFAPLYHYALAVRDNKLPIAVVNCGG
                     ISNISLILNNNEESVVGFDTGPGNALIDRLVRQRTQGKENMDTDGRYGKQGRVDKDIL
                     ALLFEKAIIKLDQNYLLQIPPKSLDYNDMTLIPELETLSLEDACATLEVFTAKTLVNS
                     LDLSSVELFPKLWVLAGGGWYNPVILNALKDLLHLKLGTDVEILTADEIGWSSQAMEA
                     QIFAYFAVRSLQNKPL"
     gene            1450..1737
                     /locus_tag="PK389_04410"
     CDS             1450..1737
                     /locus_tag="PK389_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019529038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22978.1"
                     /translation="MMYSSKNLFDFTPFIPDVLGILGVLIILWYYFLLQLGKCAAHNL
                     GFSVGNFVGAILLLISLWYNWNLASVIIEIAWCLISAYGIARYYSRTKIRR"
     gene            complement(1820..>2376)
                     /locus_tag="PK389_04415"
     CDS             complement(1820..>2376)
                     /locus_tag="PK389_04415"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HQY22979.1"
                     /translation="SYTTLNTQSPVAEAILNLGHKITADMIALGAIAGLTSTMLIFTF
                     AGTRVVYSMSKDGLIPPLFSRVHPKTQTPIRVIAISGIVMSLIAGFFPMKDVAELVNI
                     GTLAAFVIVCISAIILRITKPDLPRPFKTPFNPLIPVLGVISCGYLMISLPWTTWLRF
                     IIWMVIGIIIYAFYGYRHSKLSKK"
BASE COUNT          738 a          479 c          419 g          740 t
ORIGIN      
        1 ctgagaggtt tattttgtaa actacgtacc gcgaaataag caaagatttg cgcttccatc
       61 gcttgactgc tccaccctat ttcatctgcg gttaatattt ccacatccgt acccaatttt
      121 agatgtaaga gatctttaag cgcgtttaaa ataaccgggt tataccaccc accgccggct
      181 aaaacccaaa gctttgggaa taattctacc gaacttaaat ctaaactatt aacaagcgtc
      241 ttagccgtaa atacctctaa agtcgcgcag gcatcttcta aagaaagagt ctctaactcg
      301 gggatgagtg tcatatcgtt ataatccagc gatttaggcg gtatttgtaa taaataattt
      361 tgatctaatt taataatggc tttttcgaat aacagagcca atatgtcctt atccacccgt
      421 ccctgttttc cgtatcgacc atcagtatcc atgttttctt tgccttgcgt acgttgacga
      481 acaagacgat caattaaagc attgccggga cctgtatcaa aaccaacgac gctttcttca
      541 ttgttattca gaatcaagct aatgtttgat ataccaccac aattaacaac agcaatgggt
      601 aatttattgt ctctaaccgc taaggcataa tgatataaag gcgcaaaagg tgcaccgcag
      661 ccacccgctt caatatcacg acttctaaaa tcattaacga cagtaatgcc gatttcatct
      721 gccaattgct gaccattccc aacgataata caaattttgc gctctgggtt atgaaataaa
      781 gtttggccat gatatccaac aacatcaact tgacgcgcac tcattttttg cgcgttcagc
      841 aactttttaa cggcaatacc atgcagtttt gtagaatgtt caataatttt atctagcgta
      901 atttgcgaat tcgatctatt ttcttgataa agataaaacg ataaatctga gattttttgt
      961 tcaatatcga gatttgtgtt aatactaaag gcttctttta tataatcagg gattgcttgt
     1021 gggtaataaa ttttagcttg ttccaaattt cccgcacatt ttttaaccgc atattctgca
     1081 gattttaata atattttaaa tcgtggatca taagtgagag aaatgtgagc caacgctctt
     1141 aaaacagtag gagaaccatc tgtttcaagc aaagcggcct caatgccatc cattgaagtg
     1201 ccagacatca agccaatgct tatcataacc ttaaagaccc acttaagtta attaacttaa
     1261 tattatataa aaacatttaa agccaatata aacaaaaaca accctcatca caaccgaagc
     1321 cacacgggct tagaaataaa aaaatcagtt aaataacctc atgttttcta aagagggtcg
     1381 cgcaattttt actgtggaat gtcgttttct acttacaagg tattgaattt tgtggtatat
     1441 atatagctta tgatgtattc ttcaaaaaat ctttttgatt ttacgccctt tattccagat
     1501 gtattaggta ttttgggtgt attgattatt ttgtggtact actttttgct gcaactggga
     1561 aaatgcgctg cccataattt aggtttttct gttggaaact ttgtaggcgc aattcttctt
     1621 ttaatttcgc tttggtataa ctggaacctt gcctctgtta ttatcgaaat cgcttggtgt
     1681 cttattagcg cctatggtat agctcggtat tattcccgca caaagatacg gcgataattt
     1741 acgatattca ttatttgttg caacggtata atttcactcg ccatgacagt tttattattg
     1801 tagctctcaa cctaaaatgt tatttcttcg ataatttact atgacgatag ccataaaatg
     1861 cgtaaataat aatcccaatt accatccaaa taataaatct tagccaggtc gtccaaggca
     1921 gactgatcat taaatatccg cacgagatca cacccaaaac tgggatcaat ggattaaagg
     1981 gcgttttgaa gggtctcggt aaatcgggtt tcgtgatccg taaaataatg gcactaatgc
     2041 acacgatgac aaaagccgcg agtgtaccaa tattcactaa ttcagccaca tctttcatcg
     2101 ggaaaaaacc agcgatcaac gacatcacaa taccgctaat ggcaataact cggatagggg
     2161 tttgtgtctt tggatgaacg cgcgaaaaaa gcggcggaat taagccatct ttagacatcg
     2221 aataaaccac gcgtgtacct gcaaaagtaa agattaacat ggttgatgtt agtcctgcga
     2281 tagcacccag tgcaatcata tcagccgtta ttttatgtcc taaattcaaa atggcttcgg
     2341 caaccggtga ttgggtattc aaggtggtat atgaga
//
LOCUS       DAORVT010000186         2375 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_44816_length_2375_cov_3.474138, whole genome shotgun sequence.
ACCESSION   DAORVT010000186 DAORVT010000000
VERSION     DAORVT010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44816_length_2375_cov_3.474138"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..2017
                     /gene="asnB"
                     /locus_tag="PK389_04420"
     CDS             71..2017
                     /gene="asnB"
                     /locus_tag="PK389_04420"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HQY22980.1"
                     /translation="MCGLAGFWDFKANISKTELLGIGQKMGKAISHRGPDSEGAWCDE
                     ALGLSFSHLRLAIVDLSEAGHQPMVSRSGGSVIVYNGEIYNAPELREALSLEGCRFRG
                     HSDTEVILEACERWGVIATCQKLIGMFAFAFWDKQTQQLFLARDRLGIKPMYWGFNQG
                     ILFFGSQLKSFKGHPHFKPEIDKEALTAYFRFNYVPTPLSIFKNIQKLSPGSILSIDR
                     NQKTQEFRFWDLQDVIARAKDNRLKASCSDDALIEECDALLRDAVKRRIVVDVPLGAF
                     LSGGIDSSTVVALMQTQSDRPIKTFTIGFHEAGYDEAKHAAAVAKHLGTEHHELYLAS
                     SEAQAIIPDIPNWCDEPFADVSQIPTYLVSRLARQQVTVSLSGDGGDELFAGYNRYFV
                     GQKLWPRLKVLPYWLRLLSAQGIRQFSPNQWDQFAKILPNRLRPRLVGDKAHKLADIL
                     AVPNAGTFYKNLVSFWEEPANLVLGGNEPVLYPWNFESTFGHSNNFVESMQIMDTLTY
                     LPDDILAKVDRASMAVGLEARVPLLDHRVVEFAYGLPMNVKIRNGQGKWLLRQVLNKY
                     VPNSLIERPKMGFGVPIDQWLRGPLREWAENLLSLQRLESEGVLNPEPIRQRWAEHLS
                     GQRNWQYSLWGVLMFQAWQERWGL"
     gene            2014..2361
                     /locus_tag="PK389_04425"
     CDS             2014..2361
                     /locus_tag="PK389_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HQY22981.1"
                     /translation="MMTTRHLILISSVLCASFGHVLFKSAANTLKSLDSVWALTLEPT
                     FISALCLYGLSTLGWIWCLQEIPLTQAYLFMSLAYVFIPVMGWFFFGEVLSLQYILSA
                     GLIITGILVAIPK"
BASE COUNT          617 a          416 c          569 g          773 t
ORIGIN      
        1 caatgtatgc gcagggtatt agatattttt ccattggacg taaacccgtt ataacttcga
       61 agaaaccctt atgtgtggat tagctggttt ttgggatttt aaagccaaca tatccaaaac
      121 ggaattattg ggcattggcc aaaaaatggg taaagcaatt tcgcatcgag gcccagattc
      181 tgaaggggca tggtgtgacg aagcgctcgg tctgtctttt tcgcacctac gtcttgcgat
      241 tgtcgattta tccgaagcag ggcatcagcc catggtttcg cgttcgggtg gctctgttat
      301 tgtctataac ggagaaatct ataatgcacc tgaattgcga gaagccttaa gtttagaagg
      361 ttgtcgtttt cgtgggcatt cagatacaga agttatttta gaagcgtgtg agcgttgggg
      421 tgtaattgct acttgtcaaa agttaatcgg tatgtttgcc tttgcttttt gggataaaca
      481 gacgcaacaa ttatttttag cgcgagatag attaggcatt aagcctatgt attgggggtt
      541 taatcaaggt attttatttt ttggctcgca gctcaaaagt tttaaaggtc atccacattt
      601 taagcccgaa atagataaag aggccttgac tgcttatttt cggtttaatt atgtcccaac
      661 gccattgtct atttttaaaa atattcaaaa gctatcaccc ggtagcattt tatcgattga
      721 tagaaatcaa aaaacacagg aatttcgatt ttgggatttg caggatgtta tcgcacgcgc
      781 taaagacaat cgcttaaaag catcttgtag tgatgacgcc ttgatcgagg aatgtgatgc
      841 tttgttgcgt gatgcagtca aaagacgcat tgtggttgac gttcctctag gtgctttttt
      901 gtcgggcggt attgatagtt ctacagtcgt tgctttaatg caaacacaaa gcgatcgtcc
      961 cattaaaaca tttaccattg ggtttcatga agccggatat gatgaagcca agcatgccgc
     1021 ggcagttgct aagcatttag gtacagaaca tcatgaatta tatttagcat cgagtgaagc
     1081 gcaggccatt attcccgata ttccaaattg gtgtgacgag ccgtttgcag atgtttctca
     1141 aataccaaca tacttagttt ctagattggc gcgtcaacag gttacggtca gtctttcggg
     1201 ggatggtgga gacgagcttt ttgcgggtta taatcgttac tttgtgggtc aaaagctttg
     1261 gccgcgattg aaagttttac cctattggtt gcgacttttg agtgcacaag ggatccggca
     1321 attttcaccc aatcaatggg atcaatttgc aaaaatatta cccaatcgac tgcgtccacg
     1381 gttagtgggt gacaaagcgc ataagcttgc agatattctt gcggtaccaa atgcagggac
     1441 tttttataaa aatttagtca gtttttggga agagccggcg aatttagtgc tgggtggcaa
     1501 cgagccagtg ttgtaccctt ggaattttga atctactttt gggcattcca ataattttgt
     1561 agaatcgatg caaatcatgg atacactcac gtatttacca gacgatattt tagctaaagt
     1621 agatagagcg agtatggccg ttggtttaga agcgcgtgtt cctttgttag atcatcgtgt
     1681 tgttgaattt gcttatggtt tacctatgaa tgttaaaatt cgaaacggtc agggtaaatg
     1741 gttattaaga caagtcttaa ataaatatgt gcctaatagt ttaattgaac gccccaaaat
     1801 gggctttggt gtgcccattg atcaatggtt gcgaggtcct ttgcgggaat gggcagagaa
     1861 cttattatct ttgcagcgtt tagaatctga aggtgtttta aatcccgagc ctattcgtca
     1921 acgatgggct gagcatctgt cggggcagcg aaattggcaa tattctttgt ggggcgtttt
     1981 aatgtttcag gcttggcaag aacggtgggg attatgatga caacgcgaca tttaatttta
     2041 atttcgagtg ttttatgcgc ttcttttggg cacgtgttat ttaaatcagc agccaacacc
     2101 ttaaaatctt tggattccgt ttgggcttta accctggaac ccacttttat ttctgcgctg
     2161 tgtttgtatg gactgtctac gctgggttgg atttggtgtt tacaagaaat accgttaacg
     2221 caggcctatt tgtttatgtc gttggcatat gtgtttatcc ctgttatggg atggtttttc
     2281 tttggagaag tcctttcttt gcaatatatt ctgagtgcag gattgataat aacaggaatc
     2341 ttagttgcca tcccaaaata gatttgccta tatcg
//
LOCUS       DAORVT010000187         2367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_45065_length_2367_cov_3.399654, whole genome shotgun sequence.
ACCESSION   DAORVT010000187 DAORVT010000000
VERSION     DAORVT010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45065_length_2367_cov_3.399654"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PK389_04430"
     CDS             complement(<1..370)
                     /locus_tag="PK389_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HQY22982.1"
                     /translation="MVLVTCLAAYATTATSAPVSGPIKWHPGHYYTIMGNKNNSTYMA
                     EVYNELKSTPALRGVQIRYTWAELETSYGVYDFTSIEQRLSELSTQNKRLVILLQLKS
                     FNLRMALAPDYLKAGIYDGGV"
     gene            complement(631..1794)
                     /locus_tag="PK389_04435"
     CDS             complement(631..1794)
                     /locus_tag="PK389_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HQY22983.1"
                     /translation="MQITNPMKSKISTNLSMAARQMHSILVTILFIVSMVWVPFGTVH
                     ADGVSDVTSSSVKWHPGHYYSIMSGKKNNPAYLTQVYSELDDTPALRGVQIRYSWTEL
                     ETSYGVYDFSSIDNRLAELAAKNKRLIILLNLRIFDPDTALVPNYLENDSIYEGGIFP
                     YASGSSSTIKGYNIKLWNPMVRDRLAALFSALGKRFNSHNYFEGIGLTETAIGVPIKP
                     LTSVQVNGYYSNLLVLNQNMQDSFRNTMTYQYANYSRSILPSLIDGLKAMGAALGCPD
                     VFIEEPGLHFSGTPKGVYKYYPENSGLMPLTVQVGGSNYENTRYDGTGYQPTVSELLA
                     FAKSNLKVNYIFWQRDPDYYPKVLEMLNWSGQKSNPSGGLDPTCPMNYTSCVN"
     gene            complement(2090..>2367)
                     /locus_tag="PK389_04440"
     CDS             complement(2090..>2367)
                     /locus_tag="PK389_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018252753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HQY22984.1"
                     /translation="PEQVLRDNRGHWSIESCHYIIDWNYDEDRSQIRTGFGPENITRL
                     RRFAIGLLKDKKLNETIPERMKKLMLNTRAVFDYLKMTRNSNPGSVG"
BASE COUNT          647 a          484 c          512 g          724 t
ORIGIN      
        1 atactccacc atcgtatatt cccgctttca agtagtctgg tgcgagagcc attctaagat
       61 tgaatgattt taattgaaga agaatgacaa gccgtttatt ttgtgtggaa agttcagaaa
      121 gtcgctgctc aatggatgtg aaatcataca caccatagga tgtttccagt tctgcccaag
      181 tataacgaat ttgcaccccg cgcagggctg gcgtgctctt cagttcgttg tatacttctg
      241 ccatatatgt agaattattc ttattgccca taattgtata ataatgacct ggatgccatt
      301 tgatcggacc agatactggc gcagatgtgg cggtggtcgc gtatgcagct aagcaggtaa
      361 ctaaaaccat gcgtatgaca aataatgacg cgatcaatag agcatggttt tggctagtca
      421 caatagaggg attagtatta attaacaatt taacttgtaa ttccccgtgg tcttgccacg
      481 gggcgcacta tagattgcgt ttttcacgtt agcgtagcgc accatttttt ggttttctgc
      541 gccgtaatac cctatggtct tgatgtaggg cacacggagc aaatttattg gaattattga
      601 agtttgccaa aagtgggctt gttcaaacat ctagtttacg caggaggtat agttcatggg
      661 gcaggttgga tccagtccgc cggaaggatt gcttttttgt ccactccaat tcaacatttc
      721 cagcactttt ggataataat ccgggtctcg ttgccagaaa atgtagttga cttttagatt
      781 ggattttgca aaagcgagca gttctgagac agtaggctga tagcctgttc catcatatcg
      841 agtattctca taattcgaac cccccacttg aactgtcagg ggcatcagcc cagaattttc
      901 aggatagtat ttatagacac cttttggagt ccctgaaaag tgtaatcctg gctcttcaat
      961 gaaaacatcc gggcaaccca gtgctgcccc catcgctttg aggccgtcta ttaatgatgg
     1021 cagaattgaa cgcgagtagt ttgcgtactg gtatgtcatt gtattgcgga aggaatcttg
     1081 catattttgg tttaatacga gcagattgct gtaatagcca tttacctgaa cactggttag
     1141 cggttttata ggaactccta tggcggtttc tgtcaggccg ataccttcaa agtagttgtg
     1201 agaattaaag cgctttccca atgcgctaaa taatgcagcc agacgatcac gcaccatggg
     1261 attccacagc ttgatgttgt atcctttaat ggtggagctg ctaccactag cataaggaaa
     1321 tatgcctcct tcgtaaatac tatcattttc caagtagttt ggcacgagag ctgtgtcagg
     1381 atcaaatatt cttagattta gtagaattat aagtcgttta ttctttgcag caagttcggc
     1441 aagacgattg tcaatggaac tgaaatcata cacaccatag gatgtttcca gttctgtcca
     1501 agaataacga atttgcaccc cgcgcagagc tggcgtgtca tccagttcgc tatatacttg
     1561 cgtcagatat gcaggattat tttttttccc actcataatt gaatagtaat ggcccggatg
     1621 ccatttaact gagctggacg tcacgtcgct gacgccatcg gcatgcactg ttccgaaggg
     1681 aacccaaacc atgcttacga tgaacaatat tgtgaccaat atgctatgca tttgtctcgc
     1741 ggccatggat aaattggtgc tgatcttgga tttcatggga ttagtaattt gcatacgaaa
     1801 gactcctatt tgatattaag ataattttct caattttctg ttgaaagtga tctattaggt
     1861 tgttacgaac tgaacaaatc ttttctcgtc agagaaagga cacttcattc atagtattgc
     1921 ttacttgcgg aattttaacg ttgaaaaaag agaggtaaat atctgctttt tttgacaata
     1981 ccttggatat ttctaatatc aggcaaatgc ggaaaaaaat aaaagtacca caaaataata
     2041 aaaaagcaaa caacaaaatt ttgaaaacac ggccaatcta cgctaccccc tatcctactg
     2101 aaccagggtt agagtttcga gtcattttca gatagtcaaa gactgctcgg gtattcagca
     2161 tcagtttttt catcctttca gggatcgttt cattgagctt cttatctttg agtaaaccga
     2221 ttgcaaatcg tcgcaaccgt gtgatatttt ctggtccgaa gcccgttcga atttgactgc
     2281 gatcctcgtc gtaattccag tcgattatgt agtggcaact ttctatgctc cagtgccctc
     2341 ggttgtcccg aagaacctgt tctgggg
//
LOCUS       DAORVT010000188         2349 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_45726_length_2349_cov_2.713165, whole genome shotgun sequence.
ACCESSION   DAORVT010000188 DAORVT010000000
VERSION     DAORVT010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45726_length_2349_cov_2.713165"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1506
                     /gene="secD"
                     /locus_tag="PK389_04445"
     CDS             <1..1506
                     /gene="secD"
                     /locus_tag="PK389_04445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HQY22985.1"
                     /translation="LDLRGGVHFLMEVDVASVVTRRMEGYFSEMPKVLREAKIRYKHL
                     EHPSAETIKVFFETVDERNHACSVLQKWEQDLAISTGEIPPSAKIPPNPPLSKGGIEE
                     GSFFLVASLSPQTLLTIRNETLDQTLSILRNRVNELGVAEAIVQRQGLSRVVVELPGI
                     QDTAYARDILGTTATLKFVMEDHQHPLQDVLSGHSIVVGSKLYFDRFQRPVLLKTQPI
                     LTGEAVTGASTGQGREGDPAVSVRLGGNISLFAKTTRENVGRRMGVIYIEVKDHKTTE
                     SVISLATIQSGLGNHFQITGLSVEESRDLALLLRAGALPAPVSIVEESIIGPSMGQEN
                     IRMGMVSVAVGLGLVLVFMAIYYSVFGIIADIALLANLVLLVAIMSLIGATLTLPGIA
                     GIVLTLGMAVDANVLIFERIREEMRLGMSPKASILRGFEYAFATIIDSNLTTLIVGVV
                     LFAIGSGPVKGFAVTLCIGILTSLLTATTGSRALVELLYGGRSIKRVRVGI"
     gene            1510..>2349
                     /gene="secF"
                     /locus_tag="PK389_04450"
     CDS             1510..>2349
                     /gene="secF"
                     /locus_tag="PK389_04450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HQY22986.1"
                     /translation="MEFFKKETRIDFMGIRRYTGLLTAVLWIVALGLLFTKGINLGLD
                     FTGGTQVEARFINAPSPLQIREQLEHAGFQGVRVQQYGSTEDVLIRIGSSEKGDVDLS
                     GGSEKEQKMTARIKQILLKHYPDIEIRGTEFVGSEVGAQLVEEGGIAVLVSILATMIY
                     IALRFEYRLAVSAAVGLTHDAVLILGVFSLFGFEFDLATLASILAVLGYSLNDTIVVF
                     DRVRENFRKIRKGTPTEVMNLSINQTLSRTLMTSFMTLLVVLALLFFGGTNLFGFSMA
                     FFVG"
BASE COUNT          590 a          417 c          612 g          730 t
ORIGIN      
        1 ttagatttaa gaggcggggt acatttttta atggaagtag atgtggcttc ggtggttaca
       61 agacgcatgg aaggttattt ttccgaaatg ccaaaagtgt tacgcgaggc caagattcgt
      121 tataagcact tagaacatcc gagtgctgaa acaattaaag ttttttttga gacggtggat
      181 gagcgcaacc atgcgtgtag tgtattacaa aaatgggaac aagatttagc gattagcacg
      241 ggggaaatcc cccctagcgc caaaatcccc cctaaccccc ctttgtcaaa ggggggaatt
      301 gaagaaggat ctttcttttt agtggcgagt ttatcgcctc aaacgctact caccattaga
      361 aacgaaactt tagatcaaac cttgtcgata ctccgtaatc gcgtaaatga attaggcgtt
      421 gcagaagcca ttgtacagcg tcagggttta agccgtgttg tggtcgagct tcccggtatt
      481 caagataccg cttatgccag agatatttta ggtacaacgg ctacgctaaa atttgtgatg
      541 gaagatcatc agcatccatt gcaagatgtg ttaagcgggc actctattgt ggtgggttct
      601 aagctttatt tcgatcgttt tcaaagaccg gtgcttttaa aaacacaacc gattttaacg
      661 ggcgaagctg taacaggtgc cagtacggga caaggcagag agggagatcc cgcggtcagt
      721 gttcgtttgg gtggaaatat cagccttttt gctaaaacca cgcgcgaaaa tgttgggcgg
      781 cgcatgggtg ttatttatat tgaggtaaaa gatcataaga ccacagaatc tgttattagc
      841 ttggccacta ttcaaagtgg tttagggaat cattttcaaa ttacaggatt atcggtcgaa
      901 gaatctcgag atttagcctt gttattacgc gcaggggcat tgccagcgcc ggtttctatt
      961 gtagaagaaa gtattattgg cccgagcatg gggcaagaaa atattcgaat gggcatggtt
     1021 tctgttgcgg ttggcctggg tttagtgttg gtatttatgg cgatttatta cagtgtgttt
     1081 ggtattattg ccgacattgc cttgctcgcc aatttagttt tattagttgc gattatgtct
     1141 ttaattgggg caacgctgac gctccccggt attgcgggta ttgttttgac tttaggcatg
     1201 gcggtagatg cgaatgtttt aattttcgag cgcattcgcg aagaaatgcg attgggcatg
     1261 tcgcctaaag cgagtatttt acgcggattt gagtatgctt ttgcgaccat tatcgattcc
     1321 aatctaacca ccctaattgt cggtgtggtt ttatttgcga tagggtcggg gcctgttaag
     1381 ggttttgcgg tgactttgtg tattgggatt ttaacctcat tgttaacggc aaccacgggt
     1441 agtcgggcat tagtagaatt gttgtatggt ggccgttcga ttaagcgcgt tagggtgggg
     1501 atataaaaca tggaattttt taagaaagaa acccgcatcg attttatggg cattcggcga
     1561 tatacgggat tgctaaccgc ggttttgtgg atcgttgcct tgggattatt atttaccaaa
     1621 ggcattaatt tagggttaga ctttactggc ggtacgcaag ttgaggcgcg atttatcaat
     1681 gcacccagtc ctttgcagat ccgagaacaa ttagaacatg caggttttca gggcgttcgt
     1741 gttcagcaat atggttctac agaagatgtt ttaattcgca ttggcagttc ggaaaaaggc
     1801 gacgttgatc tcagcggtgg ctccgaaaaa gaacaaaaaa tgacggcgcg tattaaacaa
     1861 attttgttaa aacattatcc ggatattgaa attcgcggta cagaatttgt aggttctgag
     1921 gtgggcgcac aattggttga agaaggtggt attgctgtac tggtctctat tttagcgacc
     1981 atgatctaca ttgcgctccg ctttgaatat cgattagcgg tgagtgcagc ggtgggttta
     2041 acccatgatg cggttttaat tttaggtgtg ttttcactct ttggatttga gttcgattta
     2101 gcaacgctgg cttctatatt agcggtattg ggatattctt tgaacgatac cattgtggta
     2161 ttcgaccgcg ttagagaaaa cttcaggaaa attcgaaagg gcactcccac agaggtgatg
     2221 aatctttcta ttaatcagac tttgtccaga accctgatga cctcttttat gaccctgctg
     2281 gtggtattgg ctttgttgtt ttttgggggc acgaatttat ttggcttctc catggccttt
     2341 tttgtgggt
//
LOCUS       DAORVT010000189         2337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_46171_length_2337_cov_2.510079, whole genome shotgun sequence.
ACCESSION   DAORVT010000189 DAORVT010000000
VERSION     DAORVT010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46171_length_2337_cov_2.510079"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..1090
                     /gene="dnaA"
                     /locus_tag="PK389_04455"
     CDS             5..1090
                     /gene="dnaA"
                     /locus_tag="PK389_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HQY22987.1"
                     /translation="MRESVFTDNRREVPPSFEHHQSYLNPSYTFDSFVEGKSNQLARA
                     AAMQVAENPGGAYNPLFLYGGVGLGKTHLMQAVGNLIVRKNKERKARVLYLHSERFVA
                     DMVKASQSNGINEFKRRYRTVDALLIDDIQFFSGKERSQEEFFHTFNALLEGQQQIIL
                     TSDRYPKELSGIEERLKSRFGAGLTVAVQPPDLETRVAILMNKAEVSHIDLSPEVAFF
                     IAKRIRANVRELEGALKRVIANAHFTGGAITLSFVQEALKDLLALQEKLVSIDNIIKT
                     VSEYYKIKVSELLSKRRSRSVARPRQVAMALCKELTNHSLPEIGDAFGGRDHTTVLHA
                     CRLIKQLVETGSDIAEDYSNLVRTLST"
     gene            1103..2218
                     /gene="dnaN"
                     /locus_tag="PK389_04460"
     CDS             1103..2218
                     /gene="dnaN"
                     /locus_tag="PK389_04460"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009614446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HQY22988.1"
                     /translation="MRFTIQREAFLRPLRLVAGGVDRKQSSQSSPILLNTLLQVQDSH
                     FSLTTTDQEIELVAVGKLETPAEANGAVTVSVRKLMEVCKTLSEGANISFVEAEDRIT
                     LRAGRSRFMVSTLPVCEFPNLDEQVGKFRISISKKNLRLLIEQVCFAMAEQDVRYYLN
                     GMLLEVNKGYIYGVAADGHRLALGKAELCKVDSETFRVIVPRKGILELQRILDDSEEE
                     IQIILGNNHIRVITNDVQLTSKLLEGRFPDYHRIVLTDGDKIFTGCREVLKDAFQRAA
                     ALFSDRFRGVRLRLTEGYLKILAISAEQDEVEEDVEVDYVGNEFEIGFNVKYLIDLLN
                     VIQTERVKFTLSDSNNSARVEGVGAESGVYVIMPMKI"
BASE COUNT          706 a          383 c          554 g          694 t
ORIGIN      
        1 agagatcaga gaaagtgtat ttacagacaa tagacgagaa gttcctcctt cttttgagca
       61 tcatcaaagc tatttaaatc cgagttatac tttcgacagt tttgtggaag ggaagtctaa
      121 ccaattggcg cgcgcagcag ccatgcaagt cgccgaaaac cccggtgggg cctataatcc
      181 tttgttttta tacgggggcg ttgggttagg aaagacccac ttaatgcaag ctgtgggtaa
      241 tttaattgtt agaaaaaata aagaacgaaa ggcgcgtgtg ttgtatctgc attcagagcg
      301 ttttgtagcg gatatggtaa aagcgtcgca atcgaatggc attaacgaat ttaagcgacg
      361 ttatcgtaca gtcgatgctt tgttgattga tgatatacag tttttttctg ggaaagagcg
      421 atcacaagaa gagttttttc atacctttaa tgcattatta gagggtcaac aacaaattat
      481 tttaacctct gatcgttatc ctaaagaatt aagtggtatt gaagaaagat taaaatctcg
      541 ttttggtgcg ggtttaaccg ttgcggtaca accgcccgat ttagaaacgc gcgttgctat
      601 tttaatgaat aaggcggaag tctctcatat tgatttgtcg cctgaagttg ccttttttat
      661 tgctaaacgc attcgcgcga atgtgcgaga attagagggt gccttaaaaa gggtgatagc
      721 caatgcacac tttacaggcg gtgcgattac tttaagtttt gtgcaggaag ccttaaaaga
      781 tctcttggcg ctacaagaaa aattggtgtc tatagacaat atcatcaaaa cagtatcgga
      841 atattataaa attaaagttt cagaattgct ctccaaacga cgtagtcgat cggttgcgcg
      901 tccgagacaa gtggcaatgg cgctgtgtaa agaactgaca aaccacagct tacctgaaat
      961 cggagatgcc tttggtggac gtgatcacac aacagtgctg catgcatgtc gtttaattaa
     1021 acagctggta gaaaccggat cagatattgc agaagattat tcaaatttgg ttcgaacgtt
     1081 atcgacctaa aggaactttt ttatgcgctt tacaattcag cgagaagcgt ttttgaggcc
     1141 acttcgattg gtcgcagggg gggtcgatcg caaacagtct tctcaatctt ctcctatttt
     1201 attaaacaca ttactgcagg tacaagatag tcatttttca ctaaccacaa cagatcaaga
     1261 aattgaattg gttgctgttg gaaaattaga aacgccagca gaagcgaatg gagcggtaac
     1321 ggtatctgtt cgtaaattaa tggaggtatg taaaaccttg tccgagggcg caaatatatc
     1381 gtttgtagaa gcagaagatc gtatcacgtt gcgtgcgggg cggagtcgtt ttatggtatc
     1441 tacgttacct gtttgtgaat ttccaaacct agacgagcag gtcgggaaat ttaggatttc
     1501 catttccaaa aaaaacttac ggctattaat agagcaagtt tgttttgcga tggccgaaca
     1561 agacgtgcgt tattatttaa atggcatgtt gctagaagtg aataaaggtt atatctatgg
     1621 tgttgctgca gatggacata gacttgcact gggtaaggca gaattatgca aagtcgattc
     1681 agaaacattt cgcgttattg tgccaagaaa aggaatttta gaattacagc gaatattgga
     1741 cgattcagaa gaagagatcc aaattatttt ggggaacaac catattcggg ttatcacaaa
     1801 tgatgtgcag ttaacctcta agttattaga gggtcgtttt ccagactatc atcgtattgt
     1861 attaaccgac ggcgataaaa ttttcacggg gtgtcgagag gttttaaaag atgcgtttca
     1921 gcgagcagcc gcattattta gtgatcgatt taggggcgtc cgattacggt taaccgaagg
     1981 atatttaaag attttggcaa tcagcgcaga gcaagatgaa gtagaagaag atgtagaagt
     2041 ggattatgtt ggcaatgaat ttgaaattgg ttttaatgtg aaatatttaa ttgatttgct
     2101 taatgttatt caaacagaac gtgttaaatt tactttgtct gattctaaca atagcgcgcg
     2161 tgtagaaggt gttggggcag aaagcggtgt atacgttatc atgccaatga aaatttgatt
     2221 ttaagcatgt gcagcggcgg tcgcttgaaa cacaaaaaga attgtttctg attttgaagc
     2281 acggaaaagt tgcttttcca ttttcaattc attacaattt ttcgagcact gtgtagg
//
LOCUS       DAORVT010000190         2333 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_46293_length_2333_cov_3.546532, whole genome shotgun sequence.
ACCESSION   DAORVT010000190 DAORVT010000000
VERSION     DAORVT010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46293_length_2333_cov_3.546532"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /gene="nuoF"
                     /locus_tag="PK389_04465"
     CDS             complement(<1..1136)
                     /gene="nuoF"
                     /locus_tag="PK389_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HQY22989.1"
                     /translation="MTNEVCFRTRHLSEPWSLKSYESVGGYTVWRKILKEKIKPESIL
                     DELKLSALRGRGGAGFPTGLKLSFMPRNAVGQKYIVCNSDEGEPGTFKDRDILLFNPH
                     QVLEGMMIAGYVTGATVGYHYIRGEFEVPFERAENALKEARELDLLGQNILGSGVDFE
                     CHSVLGAGAYICGEETALMDSIEGKKGFPRFKPPFPANHGIYGCPTNINNTETLASIP
                     VILQHGGKWFLELGKPNNGGCKVFSVSGHVNNPGNYEVPLGIPFSELLTMAGGLREGR
                     QLKAVIPGGSSAPVLPASIMCDLTMDYDAISKAGSMLGSGAVIVIDDSACMLKILARI
                     AHFYFEESCGQCSPCREGTGWMSRIIHRIASGKGKPEDIDTLLG"
     gene            complement(1129..1629)
                     /gene="nuoE"
                     /locus_tag="PK389_04470"
     CDS             complement(1129..1629)
                     /gene="nuoE"
                     /locus_tag="PK389_04470"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012824814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HQY22990.1"
                     /translation="MALSLLSYKTKKNIDQWLTKYPSNQRQSAVLYALKIVQAENKGW
                     LTESLMDAVAAYLGMPKIAVYEVATFYNLFELKPVGKHKISVCTNISCMLCDSEGIVQ
                     YLKKRLGVKMGETTPDGQFTLKEVECLAACGGAPAVQIDGVYHEKVTPEKLEAILSEL
                     SELRDD"
     gene            complement(1632..>2333)
                     /locus_tag="PK389_04475"
     CDS             complement(1632..>2333)
                     /locus_tag="PK389_04475"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901973.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HQY22991.1"
                     /translation="NREGSLLDFIWDFTNRFPGRIDEYETLLTDNRIWKQRTVGIGVV
                     TKERALQLGFSGPMLRASGVAWDLRKKQPYAAYDQVDFDIPVGITGRGDCYDRYLVRV
                     AEMRQSNRIIRQCVEWLRQNPGPVMLDNHKITPPSRVAMKEGMESLIHHFKLFSEGYS
                     LPQGEAYAAVEHPKGEFGVYLVSDGANKPYRIKIRTAGFAHLAALDEMARGHMLADTV
                     AIIGSQDLVFGEIDR"
BASE COUNT          701 a          592 c          382 g          658 t
ORIGIN      
        1 atacccaata atgtatcgat atcttcaggt tttcctttcc ccgatgctat tctatgaata
       61 atgcgcgaca tccagcctgt accttctcta catggtgaac actgaccaca cgattcttca
      121 aaataaaaat gtgcgatgcg agcaagaatt ttaagcatac aagctgagtc gtctatcaca
      181 ataaccgcac ccgaacccaa cattgaaccg gctttgctaa tggcatcgta atccatcgtt
      241 aaatcacaca taattgatgc aggtaacacg ggtgccgaag aaccgcctgg aataacagct
      301 tttaattgac gtccttctcg taatccacct gccattgtca ataattctga aaacggtatt
      361 cccaaaggta cttcataatt accgggatta ttcacatgac ccgacacact aaaaactttg
      421 caaccaccat tgtttggctt acctaattct aaaaaccatt ttccgccgtg ctgtaaaata
      481 acgggtatag aggcgagtgt ctcggtattg ttaatattgg taggacatcc ataaatacca
      541 tgattcgcgg gaaaaggcgg cttaaatcgc ggaaaacctt ttttgccttc aatagaatcc
      601 attaatgccg tttcttcacc acaaatataa gcacccgcac ccaacaccga atgacactcg
      661 aaatcaacac cggaccccaa aatattctgt cccaacaaat ccaattctct tgcttccttt
      721 aacgcatttt cagcacgctc aaaaggcact tcaaattcac cacggatgta atgataccca
      781 accgttgcac ccgtcacata tccggctatc atcatgcctt ctaatacttg atgtggatta
      841 aacagtaaga tatcacgatc tttaaacgta cccggctccc cttcatccga attacaaaca
      901 atatattttt gtccaactgc gttacgtggc ataaaactca attttaaacc ggtcggaaac
      961 ccagcgcccc ctctgcctcg caatgccgac aactttaatt catcaagaat actttctggt
     1021 tttatctttt cttttaggat tttgcgccat accgtatacc caccaacact ttcataggat
     1081 ttcaaactcc acggttcgga taaatgacgt gttctaaaac aaacttcgtt agtcatcacg
     1141 caactccgac aattcgctca aaatagcctc taatttttca ggtgtgactt tttcatgata
     1201 aacaccatct atttgcacag caggcgcacc accacaagcg gctaagcatt ccacttcttt
     1261 taaggtaaat tgcccatcag gtgttgtttc tcccatctta acccctaggc gcttcttgag
     1321 atattgaaca atcccctcgg aatcgcagag catacaagaa atattggtgc aaacagaaat
     1381 tttatgtttt ccaacgggct ttaactcaaa taaattataa aaagtcgcaa cttcatacac
     1441 ggcaattttg ggcattccta aataagccgc aactgcatcc attaagcttt ctgttaacca
     1501 acctttattt tccgcttgga caatttttaa agcgtaaagc acagcggatt ggcgttgatt
     1561 cgatggatat ttcgttaacc attgatcaat gttttttttt gtcttgtagc ttaagagtga
     1621 aagtgccatc gtcaccgatc aatctcccca aacaccaaat cttgagagcc aataatggca
     1681 accgtatctg ccaacatatg tccacgtgcc atttcatcta aagccgctaa atgtgcaaac
     1741 cctgcagtac gaattttaat acgatagggt ttattcgctc catcggacac caaataaaca
     1801 ccaaattcac ctttaggatg ctctaccgcc gcataagctt caccttgagg taatgaatac
     1861 ccttctgaaa ataatttaaa atgatgaatt aaagattcca taccctcttt catagccact
     1921 ctagaaggcg gtgttatttt atgattgtct aacatcacag ggcccgggtt ttgccgtaac
     1981 cattcaacgc attgtcgaat aatacgattc gattgtcgca tttctgcaac acgcactaaa
     2041 taacgatcgt agcaatcccc tctacccgtt atgccaacag gaatgtcaaa atcgacttga
     2101 tcgtacgccg catagggctg tttttttcgt aaatcccaag ccacacccga agcacgtaac
     2161 atgggtcctg aaaaaccgag ttgcaaagca cgttcttttg taaccacccc aataccaacg
     2221 gttctttgct tccaaattcg attgtcggta agtaaggttt cgtattcgtc aatgcgtccc
     2281 ggaaaacgat tggtaaaatc ccaaataaaa tctaataaag acccttcgcg att
//
LOCUS       DAORVT010000191         2325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_46601_length_2325_cov_3.266960, whole genome shotgun sequence.
ACCESSION   DAORVT010000191 DAORVT010000000
VERSION     DAORVT010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46601_length_2325_cov_3.266960"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PK389_04480"
     CDS             <1..403
                     /locus_tag="PK389_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HQY22992.1"
                     /translation="TGGGVAKALPLLGSDPFIVVSSDIYTDFPYKQLPKDPEGLVHLV
                     MVDNPPHHPRGDYALVDGKICKTGQPLFNFGGIGVYRPELFVGCPEGIFPLNVLYEKA
                     IKEQQISGEYYQGLWHNIGTPTQLVDLNQRD"
     gene            540..788
                     /locus_tag="PK389_04485"
     CDS             540..788
                     /locus_tag="PK389_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HQY22993.1"
                     /translation="MLSWQLQEAKAHFSELIRACISSGPQMVLVRGKEEAVVMSKQEY
                     ERLTGKKLKLVDFMSRSPLKGLMLDLKRDTSADRDIEL"
     gene            785..1195
                     /locus_tag="PK389_04490"
     CDS             785..1195
                     /locus_tag="PK389_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017841122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HQY22994.1"
                     /translation="MSYLLDTNILSETLRLKPNALVIHFLNNIPSEALFISVLTLGEI
                     RKGIEKLETSKRKSNLIIWLEEDLSNWFGSQVLPITREVADRWGYLSAHVSRLLPAID
                     GLLAATALAHNLKMVTRNTKDFDIPGLEIVNPFD"
     gene            1263..2162
                     /gene="djlA"
                     /locus_tag="PK389_04495"
     CDS             1263..2162
                     /gene="djlA"
                     /locus_tag="PK389_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006035047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HQY22995.1"
                     /translation="MIIWGRLIGVLFGFKLLGPFGGIIGYMIGSWFDKGLRLHLHQIP
                     RARSVAVQGAFFKATFLVMGHLAKADGHVSVDEIRAAKNIMSRLELNETLKQEAMHLF
                     NSGKSAEFDLENELSNLYQECHRHSDLLRFFVEIQLEAALADGALNTNEKHVLLLICQ
                     KLHFSSEEFERLWARQWASQSFHDWFSTQFDPNARSREYHSARGYHTGGASAGSYSRN
                     QGRQTYTTPQPTVGDAYGVLGIASTATIAEIKKAYRRLMNQHHPDKLASRGLPEGMVK
                     MAKEKTQQIRAAYDLVREARGFR"
BASE COUNT          709 a          395 c          526 g          695 t
ORIGIN      
        1 aacaggcggt ggtgttgcaa aggcattacc attgttgggg tcagacccct ttattgttgt
       61 gagtagcgat atttatacgg attttcctta taaacaattg cccaaagatc ccgaaggctt
      121 agtgcattta gtgatggtcg ataatccacc acatcatccg cgtggtgatt atgcattggt
      181 tgatggcaag atttgtaaaa cgggccagcc gttgttcaat tttgggggta ttggtgtgta
      241 tagacccgaa ttgtttgttg ggtgcccaga gggcattttc ccccttaatg tattgtatga
      301 aaaagcgatt aaagaacaac aaatttcagg cgagtattat caagggcttt ggcataatat
      361 tggtacaccc acacaattag tggatttaaa tcaaagagat tgactttttc atacccaaaa
      421 taggtatgac aatgcctatt ttgggtacta ttaaaggctt gaacaagact tatccttgac
      481 gatatcctaa aaaagcgcta agcttgaaac tagtcagact agtttgtttg ggggttggta
      541 tgttgagttg gcagttacaa gaagcgaaag cacattttag tgaattaatc cgagcgtgta
      601 tatccagtgg gcctcagatg gttttggtgc gtggaaaaga agaagcggtt gttatgtcta
      661 aacaggagta cgaacgctta acgggtaaaa aactaaagct ggtagatttt atgagtcgtt
      721 ctcctttaaa aggattaatg ttggatttga agcgagatac ctctgctgat cgagatattg
      781 aattatgagt tacttgctcg atactaatat tctttctgaa acgctccgtt taaaacccaa
      841 tgcgctagtc attcattttt taaataacat accttcagaa gcactgttta tcagcgtgct
      901 tacactaggc gaaattcgaa agggtataga aaaactggaa accagtaagc ggaagagcaa
      961 tcttattatt tggttagaag aagatttgtc aaattggttt ggctcacaag tgttgcctat
     1021 tacccgggaa gttgcagatc ggtggggata tctcagtgcg cacgtatcac gcctcttacc
     1081 tgcaatcgat ggtttgttag ctgcaactgc gttagcgcat aatttaaaaa tggtcacaag
     1141 aaacacaaaa gattttgata tccccggcct tgaaattgtt aatccgtttg attgaataac
     1201 tacatttata cgatataata ctctttttgt atttttaaaa cgcaatctta aaaagtgaaa
     1261 ttatgattat ttggggcaga ttaattggag tgctatttgg gtttaaatta ttaggcccct
     1321 ttggtggtat tattggatat atgataggca gttggtttga caaaggcttg cgcctgcatt
     1381 tacaccaaat tccacgtgcg cgctcagttg ctgttcaggg agcttttttt aaagccactt
     1441 ttttggtgat ggggcatctt gcaaaagcag atggtcatgt gagcgttgat gaaattcgtg
     1501 ctgctaaaaa tattatgtca cgcttggagt taaacgagac attaaaacaa gaggccatgc
     1561 atcttttcaa ttctggcaaa agtgctgagt ttgatctcga aaacgaatta tcgaatcttt
     1621 atcaagaatg tcatcggcac tcggatttat tgcgattttt tgtagaaatt caattagaag
     1681 ctgctcttgc ggatggcgca ttaaatacga atgaaaagca cgttttacta ttgatttgtc
     1741 agaagttaca tttctcttca gaagaatttg aacgcttgtg ggcaaggcaa tgggcaagcc
     1801 agtcttttca tgactggttt tcgacacaat tcgatccaaa tgcgcgttct cgtgaatatc
     1861 acagtgctcg cggttatcac acgggtgggg ctagcgcggg tagctatagt agaaaccaag
     1921 ggagacagac atatactacg cctcagccta ccgttgggga tgcttatggg gtattgggaa
     1981 tagcatctac agcaacaatt gcagaaatta agaaagccta tcgacggttg atgaatcagc
     2041 atcatcccga taaattggct tctcgtggat taccagaagg aatggtaaaa atggccaaag
     2101 aaaaaacaca acaaatcagg gcagcttatg atttggttcg tgaagcacgg ggatttcggt
     2161 aatatcgcgt ataaattaag atagtaaagc attgaaagac gaaaaatgac cttagtctaa
     2221 tgtccaatat tttcctcata attgcaaaaa aaaaaactat actaattatt gttacgataa
     2281 ataagtgaat ctaaaagaga gagagacaat gtcaacacct aagcc
//
LOCUS       DAORVT010000192         2325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_46612_length_2325_cov_2.830837, whole genome shotgun sequence.
ACCESSION   DAORVT010000192 DAORVT010000000
VERSION     DAORVT010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46612_length_2325_cov_2.830837"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="PK389_04500"
     CDS             complement(<1..830)
                     /locus_tag="PK389_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22996.1"
                     /translation="MTIIKLGEKKSDADLEAEAQDKQDEEIEKAIADAFESTDEHLTV
                     ERINNLKNILNDIVDAQKKAKENKLTFDLKEALGFAIKKHKIVIVKKELEELLRMLNA
                     EVKNDDVASVVEVKRDEVKGLKDNQAVAAANLGTIVLSKQLIQGIDKILEENKRKDIR
                     SNLESEDKKVTADFAVDVRTILDNKKNTSGVKVNKYATSGVVVVGGACLGTGIHFMVV
                     AGSIAAAGTFPIGLAVVGGVLIIGAGIYYAKKKRERIAKFLPALYAKSKNTKDMRIEQ
                     "
     gene            complement(1048..1593)
                     /locus_tag="PK389_04505"
     CDS             complement(1048..1593)
                     /locus_tag="PK389_04505"
                     /inference="COORDINATES: protein motif:HMM:NF016189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein IspZ"
                     /protein_id="HQY22997.1"
                     /translation="MFILIRLLPLLVLYVLFFFFPFKTAMIGYVSACIVQLVLYKVVI
                     GKFSIPHFFYFLSALLFVGAALHFKHNTIFMYEISFLLLISAVYILYQSLTAKKGERY
                     LMGVLLPTDKKLASLTYLNNLMSIVYLVLALANVAIIHYLSATYWINFRVFGVYVLLM
                     FVSVVIGFGMAKSDEAQKIPS"
     gene            complement(1688..>2325)
                     /locus_tag="PK389_04510"
     CDS             complement(1688..>2325)
                     /locus_tag="PK389_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQY22998.1"
                     /translation="GTKASYLESVCQRLGQAYVRFDYSGHGASSGKFEEGTIGSWLED
                     ALCVLDNLTEGPQIIVGSSMGGWLMMLAALKRPDRIKALVGIASAPDFVEDLSHMQVV
                     QEEVLAKNGIYYLPSRDSVTVYPITPHLITEGRTHSLLKAPIPIFCPVHLLHGLSDKD
                     VPWQKSVKLAERLQSANVTVTLIKNGDHRLSERTHLALLEKSVYSLIDEAR"
BASE COUNT          715 a          528 c          362 g          720 t
ORIGIN      
        1 aattgctcaa ttcgcatatc tttagtgttt ttcgatttcg catatagcgc tggcaaaaat
       61 tttgcaatcc tttcgcgttt cttttttgca taataaattc cggcgccaat aataaggaca
      121 ccacccacca cagccaaacc tattggaaac gtgcctgcag cagctatact acctgccacc
      181 accataaaat gtattccagt tcccaagcag gcaccgccca ccacaaccac ccccgaggtg
      241 gcatacttat taactttaac gcctgaagtg ttttttttgt tatctaatat agttctaacg
      301 tcaacggcaa aatcggcagt aaccttctta tcctccgatt ctaaattaga acggatatcc
      361 ttgcgtttat tttcttctaa aattttatca ataccctgaa tcaattgttt acttaagact
      421 attgtaccta aattagcagc cgctactgcc tgattatctt tcaatccttt tacttcgtct
      481 cttttaacct caaccacgct ggccacgtca tcatttttaa cttctgcatt aagcattctt
      541 aaaagttctt ccaattcttt ttttacaatg acaattttat gtttttttat tgcgaaccct
      601 agagcctctt taagatcaaa ggtcaactta ttttctttag cttttttttg agcatccaca
      661 atatcgttta aaatattttt taaattattg atacgttcta ctgttaaatg ttcatctgta
      721 gattcaaaag catcggcaat cgctttttca atttcttcat cttgcttatc ttgcgcctct
      781 gcttccaaat cagcatcaga ttttttttca cccagcttaa tgatggtcac tgccatatca
      841 ataatacctc ttcgtgtata tccccgctta acaatgcctt attgttttgc tacttgttaa
      901 gtgctgtcaa atacagaaaa ttccacacca ttagttttat cgtacaaaaa atggaattca
      961 ttaataaaat attaggcaga tattttttaa gcatcgtatt ttgcctacag actgcagaaa
     1021 cacgaaataa tggactaaat aatacaatta cgaagggatt ttttgagctt catcggattt
     1081 tgccatgcca aatccaataa ccacgctaac gaacatcaag agcacataca cgccaaacac
     1141 tctaaaattg atccaatagg tcgctgataa gtaatggatg atggcaacat tcgcaagcgc
     1201 taagactaaa tagacaatgc tcatcagatt gtttaagtag gtcaaagaag ccagtttttt
     1261 atctgtcggc agcaataccc ccatcagata acgctcccct tttttggctg ttaaagactg
     1321 atataaaata tacacggcac ttattagcaa tagaaatgat atttcataca taaaaatggt
     1381 attgtgttta aaatgaagtg ccgcccccac aaatagtaga gcacttaaaa aatagaaaaa
     1441 atgcggaata ctgaatttcc caataacaac tttataaagt acaagctgaa caatacatgc
     1501 cgacacatag ccaatcatgg ctgttttgaa tgggaaaaag aaaaagagga catacagtac
     1561 cagtaagggg agcaaacgta ttaatataaa cataacctta accgccttaa acatcttgtt
     1621 taactaccct cagtttaggc tataaacttg cttttttctt gccctcgcgg ttgaaccttc
     1681 aattttttta tctagcctca tctatgagtg aatacacact tttctctaaa agcgctaaat
     1741 gtgtcctttc cgacaagcga tgatcgccgt ttttaattaa agtcaccgtt acattcgctg
     1801 actgtaagcg ttctgccaat ttaacagatt tttgccaagg cacatcttta tccgacaaac
     1861 catgcagcaa gtgtacaggg caaaaaatcg ggatcggtgc ttttaacagt gaatgcgttc
     1921 ggccttctgt aattaaatga ggcgtaatgg gataaaccgt tacactatcg cgtgaaggca
     1981 aatagtaaat cccattttta gcgagtactt cttcctgcac aacctgcatg tggctaagat
     2041 cttctacaaa atcgggtgcg gacgcaatac caaccaaagc cttaatgcga tcaggtcgtt
     2101 taagcgcggc taacatcatt aaccatccac ccatactaga acctacaata atttgtgggc
     2161 cctctgttag gttgtctaat acacacaagg catcctctaa ccaactgcca atggtgcctt
     2221 cttcaaactt acccgaagag gcgccatgtc cagaataatc aaaccgtaca tacgcctgcc
     2281 ctaagcgttg acaaacactt tctaaataac tggcttttgt acctg
//
LOCUS       DAORVT010000193         2301 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_47490_length_2301_cov_2.893143, whole genome shotgun sequence.
ACCESSION   DAORVT010000193 DAORVT010000000
VERSION     DAORVT010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47490_length_2301_cov_2.893143"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="PK389_04515"
     CDS             complement(<1..972)
                     /locus_tag="PK389_04515"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HQY22999.1"
                     /translation="MTRYVFVTGGVVSSLGKGIAIASLAAILESRGLKVSLLKFDPYI
                     NVDPGTMSPFQHGEVFVTEDGAETDLDLGYYERFVRTTMSKSNNFTTGRIYAEVIARE
                     RRGEYLGATVQVIPHITDEIKRNIVQDVDAFDIVLVEIGGTVGDIESLPFLEAIRQLR
                     MELGVDRTLFIHLTLVPYIPTAGEIKTKPTQHSVKELRSIGIQPDILICRSPEKLRDS
                     DRKKIALFTNVEENAVITSMDMPSIYEIPMVLHEQKLDDIVLEKLKLLAKPANLEEWE
                     KVVLHQKNPKKSIKLLLVGKYVNYADAYKSLNEALIHAGIQTETHVNI"
     gene            complement(1053..2087)
                     /gene="tilS"
                     /locus_tag="PK389_04520"
     CDS             complement(1053..2087)
                     /gene="tilS"
                     /locus_tag="PK389_04520"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HQY23000.1"
                     /translation="MKMDGNGDLVLTPELTLQNIQKLTNAKVLWVAFSGGRDSHVLLD
                     LLVRALRAGSVHNNFNPTDIQLGALHIHHGISRFADAWVQHCESVCLQYAVPFKVLYV
                     DGKVRGGQSPEAVAREARFSAFEQFLDQNQVLLLAHHAADQAETILLRLFRGSGPLGL
                     GGIREKTSLGKGELIRPLLAVSFDAIVEYAKHRKLNWIEDDSNTDIRFDRNFLRNEVM
                     PLLNARWPHAVRSTNRAGELCFEAAVVGESIAAQDLETIKGLAADALSVSRLLALDSI
                     RRRGAIRYWLQRLGHALPSRSHMERIDREVLMAKPGAKPRLKIGLYEISRHKDELKVS
                     QQTVSVRLNF"
BASE COUNT          663 a          549 c          430 g          659 t
ORIGIN      
        1 aatgttaaca tgtgtttcag tttgtatgcc tgcatggatc aatgcctcat ttagagattt
       61 ataagcatca gcgtaattaa cgtatttacc aaccaaaagt aacttaatcg attttttagg
      121 atttttttga tgtaatacaa ctttctccca ttcttcgaga ttagcaggtt ttgctaacaa
      181 ctttaatttt tctaaaacaa tatcatctaa tttttgttcg tgtaatacca tgggaatttc
      241 atagatagag ggcatatcca tggatgtaat aactgcattc tcttcaacat tggtaaaaag
      301 tgctatcttt tttctatccg aatctcgaag tttttcaggg cttcgacaaa tcaagatatc
      361 cggttgaata ccaatcgagc gtaattcttt aacggaatgt tgagtaggct ttgtcttaat
      421 ttcacccgca gtgggaatat aaggcaccaa ggttaaatgt ataaacagag ttctatcgac
      481 acctaattcc attcttaatt gacgaattgc ttccaaaaaa ggtaaggatt cgatatctcc
      541 tactgttcca ccgatttcaa ccaaaacaat gtcaaaagcg tcgacatctt gaacaatatt
      601 acgcttaatt tcgtctgtaa tatgtggaat gacttgaact gttgcgccta aatattcacc
      661 tctacgctca cgtgcaatca cctctgcgta aatacggccc gtagtaaaat tattgctttt
      721 tgacatggtg gtacgcacaa aacgctcgta atatcccaga tctagatccg tctccgcacc
      781 atcttcggtt acaaaaacct caccatgctg aaatggactc atggtgccag gatcaacatt
      841 gatataggga tcaaatttca acaggctcac cttaagacct ctcgattcta agattgccgc
      901 taatgatgcg attgctatcc ctttgcctaa cgaagaaaca acgccacccg tcacaaatac
      961 atatcgtgtc atcattgtcc ttatttccat cgtggtaaga ttttgcttaa aaagtttaat
     1021 aatagacgga gttaaatttt atcagatcgc aactaaaagt tcaaacgcac agaaactgtt
     1081 tgctggctaa ctttaagctc atctttgtgt ctactaatct catacagacc aattttcaat
     1141 cttggcttag cacccggctt agccatcaag acctcgcgat cgatgcgttc catgtgagaa
     1201 cgactgggta aggcatgccc taaacgttga agccaataac gaatggctcc tcgccggcga
     1261 atactatcga gcgccaaaag acgagacacc gaaagtgcgt ctgctgccaa cccttttatc
     1321 gtttccaaat cttgcgctgc aatgctttca cccactacag ccgcttcaaa acagagctcg
     1381 cccgcacgat ttgtagaacg aacagcatgc ggccatctcg catttaatag cggcattact
     1441 tcgtttctca gaaaatttct atcaaaccgt atatctgtat tactgtcatc ttcgatccaa
     1501 tttaattttc tatgtttagc atattctaca atcgcgtcga aagagactgc taacaatggc
     1561 ctaattaatt cgccttttcc taagcttgtt ttttctcgga tcccacctaa tcccaatgga
     1621 ccgctacctc taaataaccg aagtaaaatt gtttctgctt gatctgctgc atgatgtgcg
     1681 agtaacaaaa cttggttttg atctaaaaat tgctcaaagg cagaaaaccg tgcttctctt
     1741 gcaaccgcct ccggactttg cccacccctc accttgccat caacataaag tactttgaat
     1801 ggcactgcat actgcaaaca aacactttcg caatgttgta cccaagcatc tgcaaaacga
     1861 ctaattccat gatgaatgtg taatgcgccg agttgaatgt cggtaggatt gaagttattg
     1921 tgcacagagc cagcgcgtaa agccctcacc aaaagatcta ataagacatg tgaatcccgt
     1981 ccgccactaa aagcaaccca caacactttg gcatttgtta atttttgaat attctgtaaa
     2041 gttaattcgg gtgttagtac taaatcccca ttgccatcca tcttcatgcc cgggcagttt
     2101 tgcaggaaca gcgtagcctc acggcatgag gtcatctggc tgcacaggtt gcgcccgtcg
     2161 cacttgaagt ctgagggcac ggccagcttg ccgggcgaaa tgctggcagg cgcagcaggc
     2221 aaaaccgaat cgaacctggc tgcgcgcgtc gcgtactggc tatagccaaa ccaaagcagc
     2281 gcgcaaatga gcaagccgct g
//
LOCUS       DAORVT010000194         2280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_48284_length_2280_cov_5.180225, whole genome shotgun sequence.
ACCESSION   DAORVT010000194 DAORVT010000000
VERSION     DAORVT010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48284_length_2280_cov_5.180225"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="PK389_04525"
     CDS             <1..812
                     /locus_tag="PK389_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQY23001.1"
                     /translation="QINDIERTDLSLQSAMYGGIENGEFSLHYQPIFCAQTGRLRGAE
                     ALMRWYNPNHGFISPMKFIPLAESNGLIKILGAWALSSACHQARQWQEAGLEDFYISV
                     NVSPRQFVQEDFLEMINRVLDESGLLPENLMLEITEGVLMDNPQRSKTMLAQLHEMGV
                     KIAIDDFGTGYSSLAYLKKFSLSVLKIDKSFVDDMINSTEDMAIVGAILRLAEGLNLL
                     VVAEGVENDAQLGFLRQRGCNLVQGYLTGRPVNSAEFKEKHMVWQHNCHYL"
     gene            complement(1540..1983)
                     /locus_tag="PK389_04530"
     CDS             complement(1540..1983)
                     /locus_tag="PK389_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23002.1"
                     /translation="MGAIFFYIFIYFVGYYASNILNMLTVRPLVTNRHMAALVPVYAV
                     ALTHAYVMVSSPPPQGTDITVEYALLVFITLPVIVVTMGAAYFMWNSKAKPDDSSQTQ
                     IEKESNNVSDTEIIEKKEAIQSENIASDSEKTENQEADIIKRKDV"
BASE COUNT          741 a          383 c          462 g          694 t
ORIGIN      
        1 atcaaattaa cgatatcgaa cgcactgact tgtctctcca atcagccatg tatggaggca
       61 ttgagaacgg agaattttcg ctgcactatc agccgatctt ttgtgcccaa accgggcggc
      121 taagaggagc tgaagcattg atgcgctggt ataatcccaa tcacggcttt atatctccaa
      181 tgaaattcat tccattggca gaaagtaatg gtttgataaa aattttagga gcgtgggctc
      241 tcagcagcgc ttgtcatcaa gcaaggcaat ggcaggaagc aggcctcgaa gatttttaca
      301 tttctgtcaa tgtttctccc cgacaatttg tgcaggaaga ttttctggag atgatcaata
      361 gagtacttga tgaatcggga ctattgccag agaacttgat gttggaaatt acagaaggtg
      421 tgttaatgga taatccgcaa agatctaaga caatgctggc tcaattgcat gagatgggag
      481 taaaaatcgc tattgatgat tttggtacag gctattcatc attagcttat ttaaagaaat
      541 tttctttatc ggtattaaag attgataagt ctttcgtgga tgacatgatt aatagcactg
      601 aagatatggc gattgttgga gcaattctta gattggctga gggattaaat ttgcttgtcg
      661 tagctgaagg cgtagaaaat gatgcacaat tgggtttcct gagacagaga ggctgtaatc
      721 tagtacaagg ctacttaact ggtagaccag taaactcggc agagttcaaa gagaagcata
      781 tggtatggca acataattgc cattatcttt agtagatcct gtgccgttat gagacgtatt
      841 accatgatga aaattatcag aatccgatat caacaactcg aacactgcac acctctccaa
      901 cgcgaggatg ttctatctac aaaactaatg ctattcttat caagcatgac tgcaaaaggt
      961 tcttgagcag ttgcagcagc aacaagtcat tcgcatcaaa atcgaagcca tttcgataga
     1021 tagcatcgta ccaaatgtat tctgatggga tcgtttgtgg gaaaatgact aaaaatcacc
     1081 cgatcaggta aaaataaacg caggattaca tcgaaataaa aaaatcgcat gattttgaaa
     1141 aggcctcaga ttgtataggg gaatctgata aatctagcga tatgccagat tcaggcgaac
     1201 atcatgataa agctgatgat cagtatcaat tacaacaagt aattgatagc tgtatccgta
     1261 aaaacgatac tgaatcgatg aggatttgtc cattgtctac gagctagagt tttttcggtt
     1321 aggtcatata gagctttcaa agcttatagc ttgtcggtct cttgaagaaa tactttttgg
     1381 gaataaaacc acaaagatca atccgtcgag aataattcct attgacatct ttatttacta
     1441 tatgtcttac tttatcagtg agaagataaa gattttttta ttatctccaa cgtggttatt
     1501 agggagcttt tcacaaaaaa tcatttgaaa ctagtgcatt taaacatctt tgcgttttat
     1561 gatatctgct tcttgattct ctgttttttc tgaatcgcta gcgatatttt cggattgaat
     1621 tgcttctttt ttctcaataa tttctgtgtc gctaacattg ttagattctt tttctatctg
     1681 agtttgtgaa gaatcatcag gtttggcttt gctattccac atgaaataag ccgcacccat
     1741 cgtaacgacg attactggca aggtaatgaa taccagcaac gcatattcaa cagtaatgtc
     1801 tgttccttga ggaggaggac tgctgaccat tacatatgcg tgtgtcaatg ccactgcgta
     1861 aactggaacc aaagcagcca tgtggcgatt tgttactaac ggacgtaccg ttagcatatt
     1921 cagaatgttt gaagcgtaat atccgacgaa ataaataaaa atataaaaga aaatggcacc
     1981 catagttttc ctgtattgat ctagtttata gataagaaaa atcagatttt ataaaaaggc
     2041 cgaaaatcgt gaaacttatg atacacagat caatataaaa aatgtacaaa taaaaattag
     2101 taaagaatta ttacaagtgc agcgaatgac tgcgagtaac caagaattta acttgacata
     2161 aagtatctca gaaaaaatat attgcttagt tcgatgtatt tttgcaatga atatgaggat
     2221 gcttaatttc aatactttca ttgttggtaa atgtgagtta gaatttatag catagtaatg
//
LOCUS       DAORVT010000195         2268 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_48739_length_2268_cov_3.619521, whole genome shotgun sequence.
ACCESSION   DAORVT010000195 DAORVT010000000
VERSION     DAORVT010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48739_length_2268_cov_3.619521"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PK389_04535"
     CDS             complement(<1..325)
                     /locus_tag="PK389_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HQY23003.1"
                     /translation="MTLGERQKQFSRMIPKLLDKAHDLGFEVTLGDAFRDPRVFGAQG
                     KFAGYGRASSAHKNRLAIDLNLFKGGKYMSDTEDHKQLGEWWESQGGSWGGRFKVADG
                     NHYSLE"
     gene            complement(328..711)
                     /locus_tag="PK389_04540"
     CDS             complement(328..711)
                     /locus_tag="PK389_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HQY23004.1"
                     /translation="MSVDNLKSFNSRDMRTMPEKDPTNWTHATWALSIIMASAGGVVS
                     WYGKVKAGHTKAGNVIDLVLDVFTSAFVGTAVFMAMDAIGQPMGLCAAAGGVGGHMGT
                     RLLFIFERSVIESKLESVKVKEEDK"
     gene            complement(748..933)
                     /locus_tag="PK389_04545"
     CDS             complement(748..933)
                     /locus_tag="PK389_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HQY23005.1"
                     /translation="MADEIDITNDFYQRIEERNISGIRAAASEIPEGVAGICIECGLE
                     SKRLVGNRCAPCRDMTY"
     gene            complement(926..1108)
                     /locus_tag="PK389_04550"
     CDS             complement(926..1108)
                     /locus_tag="PK389_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23006.1"
                     /translation="MADEIDIDYREILKKYMKRIIDAEGISFVNSYERNQELSEFEIE
                     VLRKIEIELDEEIKNG"
     gene            complement(1101..1421)
                     /locus_tag="PK389_04555"
     CDS             complement(1101..1421)
                     /locus_tag="PK389_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23007.1"
                     /translation="MINVYFRTVDENGDTGYFGYASAKDGVDLFWLIDEFIDPNIVQI
                     CKANGGAGFVTKTKVDKESGYRCFKRKSEFSNGAYDVLCSEKWFTPQIVGSNIIVPWS
                     KKRG"
     gene            complement(1423..1866)
                     /locus_tag="PK389_04560"
     CDS             complement(1423..1866)
                     /locus_tag="PK389_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005773895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23008.1"
                     /translation="MIICLDLGTKTGFAAYSLHTGIVSGVKDFSTKRTEGGGMRYLKF
                     RRLLDTLNDGIEEVYYEEVKRHAGTQAAHVYGAFEGALMTWCDEHSIPYSSVPVGTIK
                     KAWTGKGNSSKDEMIKEAIRRGFEPETHDEADALALLNYVLKKVD"
     gene            complement(2005..>2268)
                     /locus_tag="PK389_04565"
     CDS             complement(2005..>2268)
                     /locus_tag="PK389_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23009.1"
                     /translation="PSWSVNVKYRRKQNINFEYAQKITAYKLLKQIDKKAAKYIKGNG
                     LKKDLNGTKNLSCLFVFNKTDEGHRYWYDLHEKLEKEIKEHGI"
BASE COUNT          587 a          586 c          339 g          756 t
ORIGIN      
        1 actctaacga gtaatggttc ccatcagcaa ctttaaacct accgccccat gatccacctt
       61 gcgattccca ccactcgcca agctgtttat gatcttctgt atcgctcata tacttaccgc
      121 ccttgaataa attcagatca atcgctagcc tgtttttgtg tgcactgcta gctcgcccgt
      181 atccagcaaa tttaccttgg gcaccgaata cacgcggatc tctgaaagca tcgccaagcg
      241 ttacctcaaa ccccagatca tgcgccttat ccagtaactt tggaatcatg cgcgagaatt
      301 gcttttgtct ttcacctaga gtcatgttta tttgtcttct tctttaactt ttacactttc
      361 aagcttcgac tctattactg atctctcaaa aataaataga agtcttgtcc ccatgtgccc
      421 acctacaccg ccagccgcag cacataaacc catcggttga cctatcgcgt ccattgccat
      481 aaaaaccgca gtgccgacga atgcgctagt aaacacgtca agaacaagat caatgacatt
      541 acctgctttt gtatgacccg cctttacttt tccgtaccaa ctgactacac cacctgctga
      601 ggccattatt atactaagtg cccatgtagc atgggtccag tttgtagggt ctttttctgg
      661 catagttctc atatctcggc tgttgaaaga ttttaaattg tctacgctca ttttctgact
      721 attccgcaat gatgtcaaga acttatttta atatgtcata tcccggcacg gcgcacagcg
      781 attaccaaca agccgtttgg attccagccc acattcaatg caaatcccag ccactccttc
      841 cggaatttca gaagccgcgg cacggattcc ggatatattg cgttcttcta ttctctgata
      901 aaaatcattg gttatgtcaa tttcatcagc catttttaat ctcctcatct aattcgatct
      961 ctatttttct aagtacttct atttcaaatt ctgataattc ttgattcctt tcgtacgaat
     1021 ttacgaatga tatcccttct gcatcaatta tccttttcat gtatttcttg agaatttctc
     1081 tatagtctat atctatttca tcagccacgt tttttgctcc aaggcacaat aatgttactt
     1141 cctacaattt gcggcgtaaa ccacttctca gaacaaagca catcatatgc tccattcgaa
     1201 aactctgatt tcctcttaaa acatcggtaa ccgctctctt tgtccacctt tgttttagtt
     1261 acaaatcctg cccctccatt tgccttacat atctgaacaa tattaggatc aataaactca
     1321 tcaatcagcc aaaacagatc aacgccgtct tttgccgatg cataaccaaa gtatccagta
     1381 tcaccatttt catctacagt tctgaagtaa acatttatca ttttaatcca cctttttaag
     1441 tacatagtta agcagcgcaa gagcgtccgc ttcatcatgc gtttccggct cgaatccgcg
     1501 ccgtattgcc tcttttatca tttcgtcctt gcttgaatta cccttaccag tccacgcctt
     1561 ttttattgtg ccgaccggca cgctggaata tggtattgaa tgctcatcac accaagtcat
     1621 gagagcgcct tcaaaagctc cgtaaacatg tgctgcctgt gtcccagcgt gacgcttcac
     1681 ctcttcgtaa taaacctcct cgattccgtc attcagtgta tcaagcaatc tcctaaactt
     1741 cagatagcgc attccgccgc cttcagttcg cttagtacta aagtctttaa ccccacttac
     1801 aatcccggta tgcagactat atgcagcgaa accagttttc gtccccaaat ctaaacagat
     1861 aatcattagt tagccaattt aatttagatt gtcattatat aattctgtta cacaatccca
     1921 aaaccaagca atgccttctc tttgacatcc tccccttcct ttagggaggg gaggatgtca
     1981 atttatattc tcaatttaag ggtgttaaat tccatgttct tttatctctt tttctagttt
     2041 ttcgtgtagg tcataccaat acctatgacc ttcatcggtt ttgttaaaca caaacaaaca
     2101 acttaaattt ttagttccat ttaagtcttt ctttaatcca ttacccttaa tgtacttagc
     2161 tgctttttta tcaatctgtt tcaaaagctt gtatgctgtt attttttgtg cgtattcaaa
     2221 attgatgttt tgttttctgc ggtattttac atttactgac cagctagg
//
LOCUS       DAORVT010000196         2264 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_48914_length_2264_cov_2.898144, whole genome shotgun sequence.
ACCESSION   DAORVT010000196 DAORVT010000000
VERSION     DAORVT010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48914_length_2264_cov_2.898144"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /gene="pgi"
                     /locus_tag="PK389_04570"
     CDS             <1..1155
                     /gene="pgi"
                     /locus_tag="PK389_04570"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HQY23010.1"
                     /translation="DLGPAMVCEALNDYKTTDIQLHFISNVDGKTLAPLLKKCNPETT
                     LCFINSKSFTTIETHQNAKALCSWFEPVLGLKTIDHLIAVTAQAKRAEAFGIAKENIF
                     EFWDFIGGRYSVWSAVGLPIALLLGMPHFKAFLAGAYEMDKHFYSAPFDQNIPVLMAL
                     LGIWNINFEEYTTQAVIPYEDGLRQFPDYLQQLEMESNGKKAQQISGFVSHATAAVIW
                     GGVGCNGQHAYMQLLHQGTQIIPVDFLVAALGHPEFEEHHQLLVASCFSQSKALMEGT
                     AENIDPNLTTDHLRDAKQCFGNRPSNTLVFPKLTPKVLGSLIALYEHKVFVQGILWGI
                     NSFDQWGVELGKTLVKKILPLLEGSVTPGKTMDLDSSTRGLIDYCLRIKS"
     gene            complement(1242..1661)
                     /gene="dksA"
                     /locus_tag="PK389_04575"
     CDS             complement(1242..1661)
                     /gene="dksA"
                     /locus_tag="PK389_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HQY23011.1"
                     /translation="MMLSYKFEPYEAKPGEVYMNEQQLKHFRNILSEKKKDLMQEVDN
                     TINEMKGKSVQVLADPNDRATEEEILNLELRARDRERKLIRKIEEALQQIDDKEYGYC
                     ESCGIEIGIRRLEARPTATLCIDCKTLDEIREKRTAQ"
     gene            1884..>2264
                     /locus_tag="PK389_04580"
     CDS             1884..>2264
                     /locus_tag="PK389_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HQY23012.1"
                     /translation="MKFKSPLIEAVLLKRSFGFLVEVLLKNKQKRMLYCPNLGSLRHC
                     DVLGSRLWFSSSNRFSQGYLDVWELTEVDGGWLVFINPGYAGILVREAIEYQLIPELA
                     GFHFLQSSMRPSFGYGIEMLLKENG"
BASE COUNT          616 a          405 c          502 g          741 t
ORIGIN      
        1 gatctcggtc ctgctatggt gtgcgaggca ttgaacgact ataaaacaac cgatattcaa
       61 ttacatttta tttccaatgt cgatggaaaa acattggcac cgcttttaaa aaagtgcaat
      121 cctgaaacaa ccttgtgttt tattaattcc aaaagcttta caaccatcga aacgcatcaa
      181 aatgcaaaag cattgtgttc ctggtttgaa cctgttttag gcctgaaaac cattgatcat
      241 ttaattgcgg ttacggcaca agctaaacgt gcagaagcct ttggcatagc gaaagaaaac
      301 atttttgaat tttgggattt tataggggga cgatattccg tttggtctgc tgttggttta
      361 ccgattgcgt tgttattagg gatgccccat tttaaagcat ttttagcggg tgcttatgag
      421 atggacaagc atttttattc agcacctttt gatcagaaca taccggtatt aatggcattg
      481 ttaggcattt ggaatattaa ttttgaggaa tacacaacac aagcggtgat tccttatgaa
      541 gatgggttgc gccaatttcc cgattattta cagcaattag aaatggaaag taatggcaaa
      601 aaggcacagc agatttctgg ctttgtatca catgccaccg ctgctgttat ttggggtggc
      661 gtagggtgta atggacaaca tgcttatatg caattattgc atcaagggac acaaattata
      721 ccggttgatt ttttagtcgc cgctctgggg catccggaat ttgaagaaca tcatcagtta
      781 ttagtggcca gttgttttag ccaaagtaaa gcgttaatgg aaggtaccgc ggaaaatatc
      841 gatccaaatt taacgacaga tcacttaaga gatgcaaaac agtgttttgg aaatagaccc
      901 agtaacacct tagtgtttcc aaaattaaca cccaaagtgt tggggtcgct tatcgcgctt
      961 tatgaacata aagtatttgt acaagggatt ttatggggca ttaattcttt tgatcaatgg
     1021 ggtgtagaat taggaaagac attggttaaa aagattctgc cacttttaga aggatcggta
     1081 acgcctggaa aaacaatgga tttagacagc tctacgcgtg ggttgattga ttattgtctg
     1141 cgtattaaat cctgaaaaat gtgttcgtag ggacggatct cgctcttttc gtaccctctc
     1201 cggcgttcgc gggagagggt ggcgcgtggc gccgggggag gtcattgcgc tgtgcgtttt
     1261 tcgcgaattt cgtctaaggt tttgcaatcg atgcataaag tggcggttgg gcgcgcttca
     1321 agacgtcgaa taccaatttc gataccacac gactcacagt acccatattc tttgtcgtct
     1381 atttgctgta gtgcttcttc gatttttcga attaatttgc gttctctatc gcgagcgcga
     1441 agttcaagat tcaaaatttc ttcttctgtt gcacgatcgt tgggatctgc tagaacctga
     1501 acactttttc ctttcatttc attaatggtg ttgtcgactt cttgcattag gtcttttttc
     1561 ttttcggaaa gaatattacg aaaatgtttg agttgttgtt cattcatata aacttcgccg
     1621 ggtttagcct cgtaaggctc gaatttataa gacagcatca tatgatcgag cacgtaagcg
     1681 tttgtcggtg gcgtaccgag agaatccgtt gactttttct ttttagcttt agcggtcatt
     1741 tggttatcct ccacctgttt taattcctta aaccttaatt tatagcacaa acccttctcg
     1801 taagcaatgg tgtacttaca cctttattta tgaacatccg agccgatgaa taagaacgca
     1861 aaccaacttt tacggttaac cttatgaaat ttaagagtcc actaatagaa gccgttttat
     1921 taaagcgttc ttttggtttt ttagtagaag tattactaaa aaataaacag aagcggatgt
     1981 tgtattgtcc caatttaggt tcacttcgac attgtgatgt tttaggtagt cgactgtggt
     2041 tctctagttc caatcgattt tctcaaggat atttagatgt ttgggaatta acagaggtcg
     2101 atggtggttg gcttgttttt atcaatccag gttatgcggg aattctcgta cgtgaagcca
     2161 ttgagtatca gctgatccca gaattagcag ggtttcattt tttacaaagt tcaatgaggc
     2221 ctagttttgg ttatggtatt gaaatgttgt taaaagaaaa cggc
//
LOCUS       DAORVT010000197         2255 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_49243_length_2255_cov_4.242727, whole genome shotgun sequence.
ACCESSION   DAORVT010000197 DAORVT010000000
VERSION     DAORVT010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49243_length_2255_cov_4.242727"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..932
                     /gene="lpxC"
                     /locus_tag="PK389_04585"
     CDS             21..932
                     /gene="lpxC"
                     /locus_tag="PK389_04585"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HQY23013.1"
                     /translation="MIKQRTLKNTIRAMGVGLHTGVKVVLTLKPAAIDQGIQFRRVDL
                     NPVVTIAASTKNVGDTTLSTTLIKDGVRVATVEHLLSAMAGLGIDNAIVEVSASEVPI
                     MDGSAGPFVFLLQSAGILEQDAPKRFLRVKRKITVSDGDKWVSLEPFDGFKVSFTLDY
                     DHPVLKNSIQEATLDFSTTSYVKEVSRARTFGFLSEYEWLRANNLALGGSLNNAIVLD
                     DYRVMNEDGLRYEDELVKHKILDAVGDMYLLGTGVLGAFRGFKSGHTLNNRLLQALLA
                     KKSAYEIVTFEDETVSPITYLYPATAA"
     gene            complement(909..1409)
                     /locus_tag="PK389_04590"
     CDS             complement(909..1409)
                     /locus_tag="PK389_04590"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HQY23014.1"
                     /translation="MEPQKHSQKHSMVEILIISPILSQIVNKVEQLAKLNRIVHQKCN
                     PELRKHCRVANYRDGILILTTHSSAVGHLLRFEKSELLTALRSLPEWCHLKSIKIHVR
                     PKPPGSEIAPLPQAGPQTTPVPLLPEHVAKDLKETALNINFPPLQHALLRLAGGFLPL
                     CSRSRI"
     gene            1444..>2255
                     /gene="secA"
                     /locus_tag="PK389_04595"
     CDS             1444..>2255
                     /gene="secA"
                     /locus_tag="PK389_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560579.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; proteins in this cluster correspond SecA1;
                     SecA2 is not essential and seems to play a role in
                     secretion of a subset of proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HQY23015.1"
                     /translation="MFITKLVRKTFGSRNERLLKRYRRVVESINALEPRMIALSDSEL
                     RDKTTEFKDRIQAGVSLEQILPEAFAVVREASKRTLKMRHFDVQLVGGIVLHEGKIAE
                     MRTGEGKTLVATLPLYLNALVGKGTHLVTANDYLAKRDAEWNQPIYEFLGLSVGVIIH
                     GLNREQRQAAYAADITYGTNNEFGFDYLRDNMVFASSERVQRGTHYAVVDEVDSILID
                     EARTPLIISGADQESSELYEHIDRLIPMLDRQKEKDAPGDYSVDEKSRQAFLT"
BASE COUNT          664 a          375 c          544 g          672 t
ORIGIN      
        1 gccaattgag ggaactcctt gtgattaaac aacgaacact caaaaatact attcgtgcaa
       61 tgggcgtagg gctacatact ggtgttaaag ttgtgttaac gctaaaacca gcagcgattg
      121 atcaaggtat tcaatttcgg cgagtggatt taaatcccgt tgttacgatt gcagcgagca
      181 ccaaaaatgt cggagataca acactctcaa cgacattaat taaagatggc gtgagggttg
      241 ctacggtaga acatttatta tcggcgatgg caggattagg cattgataat gcgattgtag
      301 aagtgagtgc gtccgaagta ccgattatgg atggcagtgc gggtcctttt gtgtttttac
      361 tgcaatccgc gggtatttta gagcaagatg cgcctaagcg atttctgcgt gttaagcgca
      421 aaatcacggt ttcagatggc gataaatggg ttagtctaga gccttttgat ggctttaaag
      481 tttcttttac tttagattat gatcaccctg ttttaaaaaa tagcatccaa gaagcgacac
      541 tcgatttttc aaccacgtct tatgtgaaag aagtgagtcg tgccagaaca tttggatttt
      601 tatccgaata tgaatggtta agagcgaata atttagcgtt aggtggtagt ttaaataatg
      661 cgattgtttt agatgattac cgtgttatga atgaagatgg attgcgttac gaagacgaat
      721 tagtcaaaca taaaatttta gatgccgtcg gagacatgta cttgttaggc acaggtgtgt
      781 tgggtgcatt tagaggattt aaatcagggc atacgctgaa taatagactg ctgcaagcat
      841 tgctggctaa aaaatcggct tatgagattg ttacctttga agatgagact gtgtctccta
      901 ttacgtactt atatcctgct acggctgcat aacggtagaa atccccccgc caaacgcagc
      961 aaagcgtgtt gcaagggtgg aaaattaata tttaaagcgg tctctttcag atcttttgca
     1021 acatgttctg gcaaaagcgg gacaggggtt gtttgaggtc ctgcttgggg taaaggcgca
     1081 atttcggatc caggtggttt tggtcgtaca tggattttaa tagattttaa atgacaccat
     1141 tctggcaacg agcgcagtgc tgttaataat tcactttttt caaatcgtaa cagatgccct
     1201 acggccgaag aatgtgttgt taaaatgaga atgccatcgc gataattagc gactctacag
     1261 tgttttcgca gttctggatt gcatttttgg tgtacaatac ggtttaattt ggctaattgc
     1321 tctactttat tgacaatttg gcttaaaatg ggagaaatta ttaaaatttc gaccattgaa
     1381 tgtttttgtg aatgtttttg aggttccata gggtaatttt aaattaaata ggcaaaaatc
     1441 accatgttta ttactaagct tgttagaaaa accttcggca gtcggaatga aagactgctc
     1501 aaacgctacc gtagagtggt tgagagtatc aatgctctcg agcctcgtat gatagcatta
     1561 tcagattctg agttgcgcga caaaacaact gaatttaaag acagaattca agcaggtgtt
     1621 tcactagaac agatattacc agaggccttt gcggtggttc gtgaagcctc caaaagaaca
     1681 cttaaaatgc ggcattttga tgttcagtta gtcggtggta ttgtattgca cgaaggaaaa
     1741 attgccgaaa tgcgaacggg agaaggaaaa accttggtgg cgaccttgcc tttgtacctt
     1801 aatgccttag tgggtaaggg aacgcattta gtgacagcga atgattatct tgcaaaacgt
     1861 gatgcggaat ggaatcagcc catttatgaa tttttgggat tgagcgttgg tgttatcatt
     1921 catggtttaa atcgagaaca gagacaagca gcgtatgctg ccgacattac gtatggcaca
     1981 aataatgaat ttggatttga ttatttacgc gacaatatgg tgtttgcatc atcagagcgt
     2041 gtacaacgcg gaacacatta tgcagtcgtt gacgaagtgg actccatttt aattgatgag
     2101 gcgcgaacac ctttaattat ttcgggtgcc gatcaagaga gttcggaatt atatgagcat
     2161 atcgatcgac ttattcctat gcttgataga caaaaagaaa aagatgcgcc aggtgattat
     2221 agtgtggacg aaaagagtcg acaggctttt ttaac
//
LOCUS       DAORVT010000198         2254 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_49286_length_2254_cov_3.427012, whole genome shotgun sequence.
ACCESSION   DAORVT010000198 DAORVT010000000
VERSION     DAORVT010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49286_length_2254_cov_3.427012"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2254)
                     /gene="rpoB"
                     /locus_tag="PK389_04600"
     CDS             complement(<1..>2254)
                     /gene="rpoB"
                     /locus_tag="PK389_04600"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HQY23016.1"
                     /translation="KPVSAAIKEFFGSSQLSQFMDQNNPLSEITHKRRVSALGPGGLT
                     RERAGFEVRDVHITHYGRVCPIETPEGPNIGLINSLAVYARTNEYGFLESPYRKVSNG
                     KVTNDIVYLSAIEEGQFVIAMANATINQKLELVDDLVPCRNKNEFTFSTPDRVEYMDV
                     SPKQIVSVAASLIPFLEHDDANRALMGSNMQRQAVPTLRAEKPLVGTGMERVVAIDSG
                     VTVIARRSGIVDSVDAARIVVRVDDSEAEAGEAGVDIYNLTKYLRSNQNTCINQKPLV
                     STGERIKRGDVLADGPSTDMGELALGQNLLIAFMPWNGYNFEDSILISERVVEEDRFT
                     TIHIEELTCIARDTKLGSEEITSDIPNVGEGSLGRLDESGVVFIGAEVTSGDILVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRVPTGMSGTVIDVHVFTRDGVEKDSRA
                     LAIEKEALLRVKKDLGDEFRIREEDIYQRVYKLLLDKVAAGGPDRLKSGSKITSTYLD
                     ELAKERWFEIRLKSDEHNQQLEAAAQQFSELRKVHDLQLEEKHKKLKQGDDLAPGVLK
                     VVKVYLAVKRRVQPGDKMAGRHGNKGVISTIVPVEDMPYLADGTPVDIVLNPLGVPSR
                     MNVGQVLETHLGWAAKGLGIKLGEMLKAQAKVAVIRQFLDKIYNLNGRRVLDLDQLTD
                     EELLVLANNLKEGIPIATPVFDGAAEEEIKQLLKLAELPESGQTVLFDGRTGVAFDRP
                     ITVGYMYMLKL"
BASE COUNT          641 a          591 c          385 g          637 t
ORIGIN      
        1 aagtttcagc atgtacatgt atcccaccgt tatcggtcta tcaaaagcaa cacctgttcg
       61 accatcaaac aagacggttt gaccactttc gggtaactcc gccaatttga gtaattgctt
      121 aatctcttct tctgctgcgc catcaaacac gggcgtcgca atggggatac cctcttttaa
      181 attgttagca agcaccaaca gttcttcatc cgttaattga tcaagatcca gaacgcgtct
      241 accatttaaa ttgtagattt tatctaaaaa ttgacgaata acggcaactt tggcctgtgc
      301 ttttaacatt tccccgagct ttatacctaa gcccttggca gcccagccca agtgtgtttc
      361 taacacttgc ccaacgttca tacgagacgg cacacccaag ggatttaaga cgatatccac
      421 gggtgtacca tcggctaaat acggcatatc ttcaaccggc acaatggtag aaataacacc
      481 tttattacca tgtcgacccg ccattttatc gcccggttga acacgacgtt ttacggccaa
      541 atacacttta acaaccttta aaacacccgg cgccaaatca tcaccttgtt ttaatttttt
      601 atgtttttct tcgagttgca aatcatgcac tttacgcaat tcactaaatt gctgcgctgc
      661 ggcttccaat tgttgattat gctcatcgct ttttaaacga atttcaaacc aacgctcttt
      721 tgccagttca tccagatatg tcgatgtaat tttgctgccc gactttaagc gatcgggtcc
      781 acccgcagca accttatcta acaacagttt gtacacccgt tgatagatgt cttcttcacg
      841 aatacgaaat tcatcgccca aatctttctt aacacgcaat aaggcttctt tttcaatcgc
      901 caatgcacgc gaatctttct cgacgccatc tcgtgtgaaa acatgcacat caataaccgt
      961 tccactcatc ccggttggta cacgtaaaga ggtatcttta acatcagagg ctttttcgcc
     1021 aaaaatagca cgtaagagtt tttcttctgg cgttaactgt gtttcaccct taggggttac
     1081 tttacccacc aagatatcac cagaagtcac ttctgcacca ataaaaacaa cacccgattc
     1141 atctaatcgt ccaagcgaac cttccccgac atttggaata tcagaagtaa tttcttcaga
     1201 acccaatttt gtatcgcgtg caatgcaggt taactcttca atatgaatcg tcgtaaacct
     1261 atcttcttca acaactcgtt cggagatcaa aatagaatct tcaaaattgt atccattcca
     1321 cggcataaac gcgattaaaa ggttttgccc aagcgctaac tctcccatat cggtagaagg
     1381 cccatctgca agcacatcgc cacgtttaat acgttcgcct gtactcacta acggcttttg
     1441 attgatacaa gtattttggt tcgatctcaa atacttggtc agattgtaaa tatcaacccc
     1501 ggcttcaccc gcttctgctt cagaatcgtc cacccgtaca acaatacgcg ccgcatcaac
     1561 agaatcaaca atcccgctac gacgtgcaat aacggttaca cccgaatcaa tcgcaacaac
     1621 gcgttccata ccggttccca ccaagggctt ttcagcgcga agcgtcggta cagcttgacg
     1681 ttgcatattc gatcccatca aggcacgatt cgcatcatca tgttccaaaa agggtattaa
     1741 agaggccgct accgaaacaa tttgtttagg cgaaacatcc atatactcaa cgcggtctgg
     1801 cgtagaaaac gtaaattcat ttttatttcg acaaggcact aaatcatcaa ccaattctaa
     1861 tttttggtta atggtcgcgt ttgccatcgc aatcacaaac tgcccttctt caattgccga
     1921 taaataaaca atgtcgttag tgactttacc gttgctcact ttacgataag gactttctaa
     1981 aaagccatat tcattggttc tcgcatacac ggccaacgaa ttaatcaacc caatatttgg
     2041 accttccggt gtttcaatcg gacaaacacg accataatgc gtaatgtgaa cgtcacgcac
     2101 ttcaaatcct gcccgctcgc gcgttaaccc gccaggccca agcgccgaaa cacggcgttt
     2161 atgtgttatt tcggataaag gattattttg atccataaat tgcgataatt ggcttgaacc
     2221 aaaaaattct ttaatcgctg ccgacacagg tttt
//
LOCUS       DAORVT010000199         2251 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_49393_length_2251_cov_4.242259, whole genome shotgun sequence.
ACCESSION   DAORVT010000199 DAORVT010000000
VERSION     DAORVT010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49393_length_2251_cov_4.242259"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="PK389_04605"
     CDS             <1..692
                     /locus_tag="PK389_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HQY23017.1"
                     /translation="AIALRLGMDPSLTIIGSSTTEQGAENITQYLEEAKIKGKGIAMD
                     IGVPESIEASIALLSESVGTPSILVNNAGITSDNLFLRLNIEEWSKVIDTNLTGTYRL
                     IHACIRTMIKQRFGRVVNISSVVGVTGNPGQTNYAASKAGIIGLSKSLAREVGSRNIT
                     VNVVAPGFIDTDMTRMLTEEQHAKLIEQIPLNRMGQPEDIAHVVAFLVSSQAGYITGE
                     TLHVNGGMYMC"
     gene            714..965
                     /gene="acpP"
                     /locus_tag="PK389_04610"
     CDS             714..965
                     /gene="acpP"
                     /locus_tag="PK389_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HQY23018.1"
                     /translation="MSNKIEDRVKDIIAENLGVDQEKVIPEASFVEDLGADSLDTVEL
                     VMALEEAFDTEIPDEDAQKLLTVKDAIDYVNARAAIETK"
     gene            981..2222
                     /gene="fabF"
                     /locus_tag="PK389_04615"
     CDS             981..2222
                     /gene="fabF"
                     /locus_tag="PK389_04615"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007467181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HQY23019.1"
                     /translation="MSKRRVVVTGMGIISPIGNQIELAWKSLLAGKSGIRSIDAFDTS
                     AFSTQFAGTVLDFDVSQFMSTKEARKCDTFIQYGVAAAAEAVRNSGLENFDALNKDRV
                     GVSIGSGIGGLGTIEANHHLLLNSGPKRISPFFIPGSIINMISGHVSINYGFKGPNMA
                     VATACTTGTHSIGLAARFIAYGDADVMLAGGAECASTPLGLGGFAAARALSTRNDAPE
                     KASRPWDRDRDGFVLGDGAGVLVLEEYEHAKKRGAVILAELTGFGMSGDAFHITRPSE
                     GGAGAVVAMQNALHDAKLNPEQIHYVNAHATSTHAGDKEETLAMKTVLGDAAKKVAIS
                     STKSMTGHLLGAAGAVEAIFTVLAIRDQVAPPTINLENPDEGCDLDYVPNIARKMKID
                     FALSNSFGFGGTNGTLVFQKI"
BASE COUNT          651 a          426 c          556 g          618 t
ORIGIN      
        1 aggccatcgc cttgcgattg ggaatggatc cctcgctcac gattattggc agttcaacaa
       61 cggaacaagg tgcagaaaac attacacaat atttagaaga agccaaaatt aaaggcaaag
      121 gcatcgccat ggatattggt gtgcccgaga gtattgaagc aagcatcgca ttgttaagcg
      181 agagtgttgg aaccccctct attttagtga ataatgcggg catcacctcc gataatcttt
      241 ttttacgcct gaatattgag gaatggtcta aagtcattga taccaattta acagggacct
      301 atcgtttaat tcatgcctgc attcgaacaa tgattaaaca acggtttggt cgtgtcgtga
      361 atatcagttc ggtagtcggt gtaacgggta atcccggtca aacgaattac gctgcctcta
      421 aagcgggtat tattggctta agcaaatcgt tagcacgaga agtgggttct agaaatatta
      481 cagtcaatgt tgtcgcgcct ggttttatcg ataccgatat gacacgcatg ctaacagaag
      541 aacaacatgc aaaattgatt gaacaaattc cattaaatcg catggggcag ccagaagata
      601 ttgctcatgt tgttgcattt ttggtttcat ctcaagccgg atacataaca ggggaaactc
      661 tgcatgtaaa tggcgggatg tatatgtgtt agtttaacgt taaggagttt tgaatgagta
      721 ataaaatcga agatcgcgtt aaagatatta tcgcggaaaa tttaggtgta gaccaagaaa
      781 aagttatacc tgaagcttca tttgttgagg atctgggtgc tgattcactg gatacagtag
      841 agctggtcat ggcattggaa gaggctttcg atacagaaat tccagatgaa gatgctcaaa
      901 aactattaac tgtgaaagat gccattgatt atgtgaatgc gcgtgccgca atcgaaacaa
      961 agtaacagag gatcatcatc gtgtctaaac gtcgagttgt agtgacaggg atggggataa
     1021 tttcccccat cggcaaccaa attgaacttg cttggaaatc gctgttggcc ggaaaaagtg
     1081 gtattcgttc gatcgatgca ttcgatacat cggcattcag tacacaattt gcgggaacag
     1141 ttctggattt tgatgtttct caatttatgt cgacgaaaga agcacgaaaa tgcgatacct
     1201 ttatacaata cggggttgcg gcagctgcag aagcggtacg taattccggc ttggaaaatt
     1261 ttgacgcgtt aaataaagat cgtgttgggg tttcaattgg atcagggatt ggtggacttg
     1321 gaaccataga agccaatcac catttattat tgaattcagg accaaagaga atttctcctt
     1381 tctttattcc cggttccatc attaatatga tctctggaca tgtttctatc aactatggat
     1441 ttaaaggccc caatatggcg gttgcaacgg cttgtacaac cggcacgcac agtatcggct
     1501 tagccgcgcg ttttattgca tacggtgatg ctgatgtgat gctggcgggt ggtgcagaat
     1561 gcgccagcac gcccttggga cttggcggtt ttgcagccgc acgtgcgtta tcaacacgta
     1621 atgacgctcc tgaaaaagcg agtcgtccat gggatcgtga cagagatggg tttgtattag
     1681 gggatggtgc aggtgttttg gtattagagg aatacgaaca cgcaaaaaaa cgaggcgctg
     1741 ttattctcgc agaattgacg ggttttggga tgagcggtga tgcatttcac attacgcggc
     1801 cttctgaagg tggcgcaggt gcagtcgttg ccatgcaaaa tgcattacac gatgcaaaat
     1861 taaatccaga acagatacat tacgttaatg cgcatgcaac ctcaacgcat gcgggtgata
     1921 aagaagaaac ccttgccatg aaaacagtat taggggacgc tgctaaaaaa gtggcgatca
     1981 gttcgaccaa gtctatgaca ggccatttgt tgggtgctgc cggtgcagtg gaagctattt
     2041 ttacggtatt agccatccgc gatcaagttg caccaccgac cattaattta gaaaacccgg
     2101 atgaaggctg cgatctcgat tacgttccta atattgcaag aaagatgaaa attgattttg
     2161 ctttatccaa ttcttttggt tttggtggca caaacggtac gttagtattt cagaaaattt
     2221 agtttgaaat cccctcgacc tgttagatca a
//
LOCUS       DAORVT010000200         2248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_49522_length_2248_cov_3.278158, whole genome shotgun sequence.
ACCESSION   DAORVT010000200 DAORVT010000000
VERSION     DAORVT010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49522_length_2248_cov_3.278158"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PK389_04620"
     CDS             complement(<1..594)
                     /locus_tag="PK389_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HQY23020.1"
                     /translation="MAHVSDLIAEDIELYLRTHENKSLLRFITCGSVDDGKSTLIGRL
                     LYESKMLFEDQLAQLEVDSKKVGTQAGDLDFALLVDGLAAEREQGITIDVAYRFFSTD
                     KRKYIVADTPGHEQYTRNMITGASTADVAVILIDARKGLLTQTRRHSYLVSLIGIRHV
                     VLAINKLDMVGYSEEIYNQIDIEYRIFAKEVGLQNIVT"
     gene            complement(594..1490)
                     /gene="cysD"
                     /locus_tag="PK389_04625"
     CDS             complement(594..1490)
                     /gene="cysD"
                     /locus_tag="PK389_04625"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HQY23021.1"
                     /translation="MLTHIQRLEAESIQIMREVVAECEHPVMLYSIGKDSAVMLHLAV
                     KAFFPSKLPFPLLHVDTTWKFKEMIEFRDVMVKKLGLDLIVHINPHGIEKGINPFTHG
                     SAVHTDIWKTEGLKQALDKHGFDVAFGGARRDEEKSRAKERIFSIRSAQHRWDPKLQR
                     PELWRLYNTRKNKGESIRVFPLSNWTELDIWQYIYLNNIPIVPLYFAKERFVVERDGT
                     LIMVDDDRLPMLKDEVPMLKKVRFRTLGCYPLTGAIESEANSLTAIIQEMLLAKTSER
                     QGRLIDHDSAGSMEKKKQEGYF"
BASE COUNT          663 a          506 c          415 g          664 t
ORIGIN      
        1 agtcacaata ttttgcaaac cgacttcttt tgcaaaaata cgatactcaa tatcaatttg
       61 gttataaatt tcttccgagt accccaccat atccagttta ttaattgcca gaaccacatg
      121 acgaattccg atcagcgaaa ccagatagct atgccgccgg gtttgtgtca ataagccttt
      181 ccgagcatcg ataagaataa ccgcgacatc agccgttgag gcgccggtaa tcatattgcg
      241 tgtatactgt tcatgccctg gcgtatccgc cacgatatac tttcgcttat ccgtcgaaaa
      301 aaaacgatac gccacgtcaa tggtaatgcc ttgctcgcgt tctgccgcga gcccatccac
      361 cagaagcgca aaatccagat ccccagcttg tgtacccact ttctttgagt ctacttccaa
      421 ctgggccagt tggtcttcaa ataacatttt tgactcataa agcaaccgtc caatcagtgt
      481 gctttttccg tcatcaacac tgccgcaggt gataaagcga agtaagctct tgttttcgtg
      541 agttcttaaa tagagctcga tatcctcggc aatcaaatca gacacatgcg ccactaaaag
      601 taaccctcct gtttcttttt ctccattgaa ccggcagaat catgatcgat aagacgccct
      661 tgccgttcgg atgtttttgc aagcagcatt tcttgaatga tcgccgttaa agaattcgcc
      721 tcgctctcaa tcgctccggt caatggatag cagcccagcg tacgaaaacg cacttttttc
      781 agcatgggaa cctcatcttt aagcatcggc aaacgatcat catccaccat aatcaatgtg
      841 ccatcacgct ctacaacaaa gcgttcctta gcaaaataca gtggtacgat gggaatatta
      901 ttcaagtaga tatactgcca gatatcgagc tcagtccagt ttgaaagagg aaatacccga
      961 atactttcac ctttattttt acgcgtgtta tacagccgcc atagttcagg ccgctgtaac
     1021 ttgggatccc atcgatgttg cgccgaacgg attgaaaaaa tacgctcctt ggcacgcgac
     1081 ttttcctcgt cccggcgtgc gccaccaaat gcgacatcaa agccatgttt atctagcgcc
     1141 tgctttagtc cttccgtttt ccatatatca gtatgaactg cagaaccatg tgtaaatgga
     1201 ttaattcctt tttcgatccc gtgcggatta atgtgaacaa tcagatcaag tccgagcttc
     1261 ttcaccatta catcacgaaa ttcaatcatt tctttaaatt tccatgtggt atcaacatgc
     1321 agtaacggga aaggcagttt cgatggaaaa aatgccttta ctgcaagatg cagcattact
     1381 gcactatcct tgccgataga atagagcatt accgggtgct cacactccgc aacaacctcc
     1441 cgcatgattt gtatgctctc cgcttcaaga cgctgtatgt gtgtcaacat tatcaattgc
     1501 ttccttcaat agtaaaacgt agcagcatca tattaatatg aaaatggatt tttcttattg
     1561 cgaaataaac tattttatcc ctgtaatctc aattcttctt acatttaggg aaataatttg
     1621 tatgccggtc agcatgttta tttttggcag ataattgcgc acgtcattta gattattctg
     1681 atttaataga attctttaat tactgtagag cgcctttaga aattcaaagt tctaaacaaa
     1741 acgaggcgtg agcaacaaaa tgttgtgacg aaatttggat ttttagaggt gccctgtatt
     1801 aaaagaacaa ggaattcact gtagctctca aacaaattct tctatttatt ttagcaaata
     1861 ttgtatacga tactttcaat attatcagta aacaagagcc attgccccaa acaaactatc
     1921 tgtttgaagt acatgtgatg aatggcgatt gtccgagaac agtgatggta gcgaggggaa
     1981 accattttga gacagatttt ttgattattt taggcgaata gttgttctat ttaacgagag
     2041 taatcggaaa atatgatcaa aatagccttt cctcagaaga ttactctatt ctcgaacagc
     2101 ctagatatgg gctcattcat tattatgaat ttaacagaaa tactatacag ggcacctcta
     2161 aaaattcaaa gttctaaaca aaacgaggca cgagcaaaaa agtgttgtga cgtaatttgg
     2221 atttttagag gtgccctaca gatctttt
//
LOCUS       DAORVT010000201         2246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_49609_length_2246_cov_2.863989, whole genome shotgun sequence.
ACCESSION   DAORVT010000201 DAORVT010000000
VERSION     DAORVT010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49609_length_2246_cov_2.863989"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..1167
                     /gene="rlmN"
                     /locus_tag="PK389_04630"
     CDS             22..1167
                     /gene="rlmN"
                     /locus_tag="PK389_04630"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HQY23022.1"
                     /translation="MPSCELKNNPKTNLLELDLESLSKYFIAQGEKSFRAKQVFQWIH
                     QRGVDNFAEMTDLSKILRERLSAECDLRAPEIVKEQCSLDGTRKWLMRLDSGNNIETV
                     FIPEKNRGTLCVSSQVGCALNCTFCSTATQGFNRNLTIAEIIGQVWQASRRLRTLPVS
                     EVHKNVITNVVMMGMGEPLLNFDAVVAALSMMRDDLAYALPRRRVTLSTSGVVPMIDR
                     LSFSADVSLAVSLHAPNDELRSQLIPLNKKYPIAILMDACRRFVSDKPGRHVTIEYVM
                     LKGINDKPEHAKQLVKLLQGLPCKVNLIPFNPFSGADYERSLVEDMDVFLAILKKAGY
                     IATLRKTRGDDIDAACGQLVGKITDRTRRNARFVAKTVEIRVEEEAA"
     gene            1190..2017
                     /gene="pilW"
                     /locus_tag="PK389_04635"
     CDS             1190..2017
                     /gene="pilW"
                     /locus_tag="PK389_04635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HQY23023.1"
                     /translation="MVIKRCHCLFVIMSLLGLFSCAQSTTQKSEKTRESEQRRIAKLL
                     PDDAAMGTPDYHRAALINVELGLGYLSQGQVARAKTKLTHAVQLAPKIPETHSAMAYF
                     LEMIGEFKDAEREHKKAVGFSGQGAVYNNYGAFLCRRGRLKEADQAFHAAIEDKEYPR
                     TAEVYENAGLCALKWPDDRKATEYLTIAVRRDPSRSSAFLELAALSLKQEKFEEAKEW
                     LNRYRAIADQTARSLWLGIGVSKGLKDENSAESQAATLKNLFENSPEYQLYLKSGKS"
     gene            2020..>2246
                     /locus_tag="PK389_04640"
     CDS             2020..>2246
                     /locus_tag="PK389_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HQY23024.1"
                     /translation="MAKKFEGVRGMPDVLPGDTPRWRQFERCLQRVVAAYGYEEIRLP
                     ILEPTGLFTRSIGELTDIVEKEMYTFRSLSD"
BASE COUNT          671 a          396 c          551 g          628 t
ORIGIN      
        1 acaagaacta cattccagat gatgccatct tgcgagctca aaaataatcc aaaaacaaat
       61 cttttggaac tcgatttgga atccttgtcg aaatatttta ttgcgcaggg tgaaaaatcg
      121 tttagagcaa aacaagtttt tcagtggata catcaacgcg gtgttgataa ctttgctgag
      181 atgaccgatt tgagcaaaat attgcgagag cgcttgtctg cagaatgtga cttaagggcg
      241 ccagaaattg ttaaagaaca atgttcgttg gatggtacgc gtaaatggtt gatgagactg
      301 gatagcggga acaatattga aaccgttttc attccagaaa aaaacagagg gactctctgc
      361 gtttcttctc aagtgggttg cgcattgaat tgcacatttt gttctacagc aacacagggg
      421 tttaatcgaa atctcaccat agccgaaatt attggacaag tgtggcaagc cagtcggcgt
      481 ttacgaacat tacccgtaag tgaagtacac aaaaatgtga ttaccaatgt cgttatgatg
      541 ggtatgggtg aacctttatt aaattttgat gcagttgtgg ctgcgttatc aatgatgcgt
      601 gatgatttag cgtatgcatt acctcgcaga cgcgttactt taagtacttc tggtgttgtt
      661 ccgatgattg accgtttaag tttttcagcg gatgtgtcgc ttgctgtttc gctgcatgcg
      721 cctaatgatg aactgcgaag ccaactgata cccttgaaca agaaataccc cattgctatt
      781 ttaatggacg cttgtaggcg atttgtatca gataagccgg gtcggcatgt tactatcgaa
      841 tatgtgatgc tgaaagggat aaatgataaa cctgaacacg ccaaacaatt ggttaaactg
      901 ttacaggggc ttccttgtaa ggtaaattta attccattta accctttctc aggtgcggat
      961 tatgagcgat ctttggttga agatatggat gtatttttag cgattttaaa aaaagcggga
     1021 tatatagcaa ctttgcgtaa aacccgtgga gacgatattg atgcggcatg tgggcagctt
     1081 gttggaaaaa ttaccgatcg aacacgtcga aatgcgcgtt ttgtcgcaaa gactgtcgaa
     1141 attcgggttg aagaggaagc agcgtagtca tctcaaggag agtttagtta tggttataaa
     1201 aagatgtcac tgtttgtttg taatcatgag tttgttagga cttttctctt gtgcgcaatc
     1261 cacaacacag aaatcagaaa agacgagaga atccgagcag aggcgtattg cgaaattatt
     1321 gcccgacgat gctgcaatgg gtacgccaga ttatcatcgt gctgcattaa ttaatgtaga
     1381 attgggttta gggtatttaa gccaaggcca ggttgcgcgt gcaaaaacaa aactaaccca
     1441 cgcagtacaa ttagcgccca aaattcctga aacccactct gcgatggctt attttttaga
     1501 aatgataggc gaatttaaag acgcagagcg cgagcataaa aaggcggtgg gtttttcggg
     1561 gcagggagct gtgtataata attatggcgc ttttttgtgt aggcgcggtc gtttaaaaga
     1621 agctgatcag gcatttcacg ctgcaataga agataaagaa tatccgagaa ctgcggaagt
     1681 gtatgaaaat gcagggcttt gtgctttaaa atggccagac gaccgcaaag caaccgaata
     1741 tttaacaata gcggttcgtc gagatcccag ccgttcatct gcttttctgg aattagcggc
     1801 actgagtctt aagcaagaaa aatttgaaga agcaaaggaa tggttgaatc gttatagagc
     1861 cattgccgac caaacagcgc gatcgttatg gttaggtata ggggtatcta aaggcttaaa
     1921 agacgaaaac agtgcagaaa gtcaagcggc aacgttgaaa aatctgtttg aaaattcacc
     1981 tgaatatcaa ttgtacctaa agtctgggaa aagctgaata tggcaaaaaa atttgaaggt
     2041 gttcgtggca tgcccgatgt tctccctggg gatacaccgc gttggcgcca atttgaaagg
     2101 tgcttacaac gggttgttgc tgcttatggc tatgaagaaa ttcgtttgcc tattttagag
     2161 cccaccggcc tttttacacg atcgattggt gaattgacgg atattgtcga gaaggaaatg
     2221 tatacattcc gaagtttgtc ggataa
//
LOCUS       DAORVT010000202         2237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_49980_length_2237_cov_3.030706, whole genome shotgun sequence.
ACCESSION   DAORVT010000202 DAORVT010000000
VERSION     DAORVT010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49980_length_2237_cov_3.030706"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..620)
                     /locus_tag="PK389_04645"
     CDS             complement(27..620)
                     /locus_tag="PK389_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23025.1"
                     /translation="MRTELEMLDWSDVREAVQKVNPRLVNIIDNLKLSPQHKLIKATY
                     AFGDLIVKEGHLCLPIQESKLYTQLNYSPIPLSLLLNKSSEVFVQNHDSALPLNRLQP
                     GNFFGTFEAVNFMMHKPAASIWHVSAGARSIFMLPKISDLSGIKRLRAHYPIAHNLHL
                     RSLSDHWSLFRQITQKPLFDFDWKSEVLFFSESMAYE"
     gene            complement(668..988)
                     /locus_tag="PK389_04650"
     CDS             complement(668..988)
                     /locus_tag="PK389_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23026.1"
                     /translation="MVKLIYLIMLLSVYLPWHFGWDMALVILPPKILGAMHYRHLCPS
                     YHPKKAYQAKHLRKTFVSAAGLFAFFRLDSWGGIFKCLFPRTIVNPNFEVSRGKHATY
                     PIRF"
     gene            complement(1522..1794)
                     /locus_tag="PK389_04655"
     CDS             complement(1522..1794)
                     /locus_tag="PK389_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23027.1"
                     /translation="MSSNIVADYLNFIKEQIVLHKQLLGCHCKAYSTLEILLRSNLND
                     YPASIIHGCLSGVSDDLRQAKALNESLLNVLTTLVQVSEPVKKIFQ"
     gene            complement(1956..>2237)
                     /locus_tag="PK389_04660"
     CDS             complement(1956..>2237)
                     /locus_tag="PK389_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23028.1"
                     /translation="NLSASTCPVALSWYRTDFVFFFWEQVDQWVQAQGLDTKTCESVL
                     KPSNGLHFKDAKNALYDRVAEKHHLDFIDYLIDCEVTSFESEDSVDEEY"
BASE COUNT          683 a          479 c          431 g          644 t
ORIGIN      
        1 tataaaatgg atacaaagca ggacttttat tcataagcca tactttcgga aaaaaaaagc
       61 acctcagatt tccaatcgaa gtcaaaaaga ggcttttgcg tgatttgtct aaaaaggctc
      121 caatggtccg ataaacttcg aagatgcaag ttatgtgcga taggataatg agcacgtaag
      181 cgtttaatgc cggataaatc gctaatttta ggcaacataa aaatagagcg cgcccccgca
      241 gaaacatgcc aaattgaagc agcgggtttg tgcatcataa aattgaccgc ttcaaaagta
      301 ccaaaaaaat tgcctggctg aagcctattt aaaggcaaag cactgtcatg gttttgtaca
      361 aaaacttcac tagatttatt cagcaacaga gacaagggaa tggggctgta atttaattgc
      421 gtgtataatt tgctctcttg gattggcaaa cataaatggc cttctttaac gattaaatca
      481 ccaaaggcat aagttgcttt aataagctta tgctgagggc taagttttaa gttgtcaata
      541 atgttaacaa gcctgggatt gactttttgc acggcttccc gaacatctga ccaatcgagc
      601 atctcaagtt ctgtacgcat acgctacacc ttttgggaat atagaattgc ccgcgggtac
      661 gaatgcgtta aaaccgtatt gggtatgttg catgcttgcc ccgcgacact tcaaaatttg
      721 gattgacaat cgttcgcggg aacaagcatt tgaaaatacc gccccagcta tccaacctaa
      781 aaaacgcgaa caagcccgcc gcagaaacaa aggtttttct gaggtgtttg gcttgatagg
      841 ctttctttgg gtggtagctg gggcataaat gccgataatg catagcacca aggattttag
      901 gtggaagtat gactaaagcc atatcccagc caaaatgcca aggcaaatat acagacaaca
      961 acataataag gtatattaat ttcaccatgc tggttcctaa tacgaactag ggtggtaccg
     1021 taactgggga tgtgccaaca tcccagagta cggcctagct cctctccttt gaggggctag
     1081 ggttttttaa ctctaccgca aaaatcagcg ctaatcaact tttaaataag cggttttacc
     1141 ggaatccaag tgccgttttc gtcttgttta acctctacca ggattgaaac ccgaactgaa
     1201 tttaaatgtt gatgcggttt tgcctgcata ctacacagac cacaggcaga tgagtttcgg
     1261 atcctttaaa ccttcaacga attccgcata cgctttacgc cagttctgta cttttctacc
     1321 atgcaggtat aaagtgtttt ctttgatgct cttaaatcaa agtcttctcc gctttttaaa
     1381 atcctgcttt cttggatcca gtccagtatt aaacgttcaa aaaataagca gaagtcctga
     1441 tcgaattttt ctaaagcagc aatacggtta tgatagccgg gccaatattc cgcgcctatt
     1501 tttacactgc ttattaagcg attattgaaa gattttttta acaggctctg atacctgtac
     1561 cagcgttgtt aaaacgttta acaacgattc attcagtgcc tttgcctgtc ttaggtcgtc
     1621 gctaacccca gataaacacc cgtgtatgat tgaagctggg taatcgttta aattagagcg
     1681 taacaatatt tccaaggtgc tataggcttt gcaatggcac cctaacaatt gcttgtgtaa
     1741 aacaatttgc tcttttataa aattcaaata atctgctaca atgttgctac tcataattaa
     1801 ctctccgtga ttgattgtga caacgataag tagatgggct acaccaccta ttcatcgtgg
     1861 ttgtggcata tgggcaatac ccatatgcca tggctctatg caataaacag tgtattcatt
     1921 tgctcacctc cctaaaaatg ctcaatatgt gggctttagt attcttcatc aacactgtct
     1981 tctgactcaa agcttgtaac ttcgcaatca atcaaatagt caataaaatc caagtgatgc
     2041 ttttctgcaa ctctgtcata caaagcattc ttggcatctt taaaatgcag cccattagat
     2101 ggttttaaaa cgctctcaca agtcttagta tctaaaccct gagcctgaac ccattggtca
     2161 acctgttccc aaaaaaagaa tacgaaatca gtcctatacc aagacagcgc cacaggacaa
     2221 gtcgaagcgc ttaagtt
//
LOCUS       DAORVT010000203         2233 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_50143_length_2233_cov_2.980716, whole genome shotgun sequence.
ACCESSION   DAORVT010000203 DAORVT010000000
VERSION     DAORVT010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50143_length_2233_cov_2.980716"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="PK389_04665"
     CDS             <1..1170
                     /locus_tag="PK389_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQY23029.1"
                     /translation="QNKIVIITGGPGVGKTTLVNSILKIICAKTNKVLLGAPTGRAAK
                     RLSDSTGLEAKTLHRMLEFDPQGHGFRRDEHNPLEADLVVIDEMSMVDITMMNHLLKA
                     IPDHAGLLLVGDVDQLPSVGPGAVLANLIDSEKIPTVRLTEIFRQAKTSQIVVNAHRV
                     NQGYMPEIPAVSAELTDFYLIPADTPELIHEKLMQVVTERIPLRFGFDPIRHIQVLVP
                     MNRGGLGARALNIDLQKRLNPNGKNAITRFGSSYAEGDKVIQTINNYNKEVFNGDIGF
                     ITAINLETSLITVLFEGRLVDYEFDEVDELSLAYATTIHKAQGSEYPAVVIPIATQHY
                     MLLQRNLLYTGLTRGKSLVVIIGQLKAVGMAVRTLRSTKRLTNLTNRLVGHCTAV"
     gene            1272..1556
                     /locus_tag="PK389_04670"
     CDS             1272..1556
                     /locus_tag="PK389_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23030.1"
                     /translation="MKNLLISGLVVGSLIACPVAFGAAEQKTEPKAEQTQQTVEPAQS
                     PAQAPAKHKSKGHKKHRAKKHHRKHRAKHMTTTETPAEKKIDQETELTNR"
     gene            1732..>2233
                     /locus_tag="PK389_04675"
     CDS             1732..>2233
                     /locus_tag="PK389_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002685605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HQY23031.1"
                     /translation="MQKLKVSYTCTECGALSPKWSGQCGDCEAWNSLTEVAMTAKPSS
                     TNQNPRFKGYAGLEETQVIELAAVPTQHENRISSGLSELDRVLGGGLVSGSAILIGGD
                     PGIGKSTVLLQMLAHLGKQHRALYVTGEESLQQIKLRAERLGVGDFGLQLLAETQVER
                     ILEIALR"
BASE COUNT          676 a          407 c          522 g          628 t
ORIGIN      
        1 caaaataaaa ttgtgattat tacaggaggg ccgggggtgg gtaaaaccac cttagtgaac
       61 agcattttaa aaatcatttg tgccaaaacg aataaagtat tattaggtgc gccgacgggc
      121 agagctgcaa aacggttgag cgattctaca gggctagaag ccaaaacttt gcatagaatg
      181 ttagagtttg atccacaagg acatggattt agacgagatg aacacaatcc attggaagca
      241 gatctggttg tgatagatga aatgtcgatg gtggatatta caatgatgaa ccatctctta
      301 aaagcgattc ccgatcacgc aggtcttttg ttagtgggtg atgtggatca attaccttcg
      361 gttggccctg gcgccgtatt agcgaattta attgattcag aaaaaattcc aaccgttcga
      421 ttaactgaaa tatttcgaca agcgaaaacc tcacaaattg tggtgaatgc ccatcgtgtg
      481 aatcaagggt atatgcctga aattcctgct gtttctgcgg aattaacaga tttttatttg
      541 atccccgccg acacgccaga actgattcac gaaaaattaa tgcaagtggt gaccgagcgt
      601 attcctctac gttttggttt tgatccgatt cgtcatattc aagtattggt tccgatgaat
      661 cgtggtggtt tgggtgcaag agcattgaat attgatttgc agaaacgtct taatccaaat
      721 ggaaaaaatg ccattacacg ctttggatct tcctatgctg aaggcgataa ggttattcaa
      781 acaattaata attacaataa agaagtattt aatggagata ttggatttat taccgctatc
      841 aatctagaaa ccagtttaat cacggtttta tttgaaggac gtttggttga ttacgaattt
      901 gatgaagttg atgaactttc tctggcttat gcgacaacca ttcataaagc acagggatca
      961 gaataccctg ctgttgtaat ccccattgcg acacaacatt atatgctact gcaaagaaat
     1021 ttattgtata caggtttaac gagagggaag tctttggttg tgattatagg tcaattaaag
     1081 gcagtgggta tggccgttag aaccttacgg tcgaccaaac gcctgacgaa tcttacaaac
     1141 cggcttgtgg gtcattgtac ggccgtttaa cttgcgttta acgttatgga gttgaaggaa
     1201 tatttttttt gagctacttt taactttatg tgtttgtaag ttttttttaa tttagacaag
     1261 gagagttgac tatgaaaaat ttattgatca gtgggttggt ggtaggttct ttgatagcct
     1321 gtcctgttgc ttttggagca gcggaacaaa aaactgaacc aaaagccgag caaacacagc
     1381 aaacagtgga gcccgcacag tcccctgcac aggcgcctgc aaagcataag agcaaaggtc
     1441 ataaaaaaca ccgtgctaag aagcaccatc gcaagcatcg tgcaaaacac atgactacaa
     1501 cagaaacgcc tgctgagaaa aagatagacc aagaaacaga actgaccaat cgttaaggtt
     1561 ttggtttgga ataaaccaat cgtggtcgca tgatggggga gaggaatacc cgttttcctt
     1621 ccttgttgag atagagccgg acgttatctc tgatctccct catcggctta aagcaaattg
     1681 ccattacctt ttttatcgcc ataaatccct taaaagttga tacaatgggg aatgcaaaaa
     1741 ttaaaagtgt cttatacctg tacggaatgt ggtgcgctta gtcccaagtg gagcggtcaa
     1801 tgtggtgatt gcgaggcttg gaatagctta acagaagtcg ctatgacagc caaaccgtct
     1861 tctacaaatc aaaaccctcg ttttaaaggc tatgcgggct tagaagaaac ccaggtgatt
     1921 gaattagcgg cagtgccaac gcaacacgag aaccgcattt cttcggggct atctgagtta
     1981 gatcgtgttt tagggggcgg cttagtttca ggttctgcaa tcttaattgg cggagatcca
     2041 ggtattggga agtcgactgt tctattacaa atgttagcgc atttgggtaa acagcataga
     2101 gcattatatg taacaggcga agaatcatta caacagatca aattgcgcgc agaaagattg
     2161 ggtgttggtg attttggttt acagctgttg gcagagacac aagttgaacg cattttagaa
     2221 attgcactac gcg
//
LOCUS       DAORVT010000204         2232 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_50175_length_2232_cov_3.473128, whole genome shotgun sequence.
ACCESSION   DAORVT010000204 DAORVT010000000
VERSION     DAORVT010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50175_length_2232_cov_3.473128"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..2110)
                     /gene="ligA"
                     /locus_tag="PK389_04680"
     CDS             complement(62..2110)
                     /gene="ligA"
                     /locus_tag="PK389_04680"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HQY23032.1"
                     /translation="MKKVNPKTDIDDKTLNRAKALRSELNEHNYRYHVLDAPTIPDSE
                     YDRLFQELKQLESQHPELKTMDSPTQRVGASPLKDFPEVTHVVPMLSLDNAFSEETVR
                     DFDKRIHARLKTENVIQYTCEPKLDGLAITLLYENGIFMMGATRGDGVSGEKITENLR
                     TISAIPLKLHGTKVPPLLEVRGEVFMPKAGFEALNKNATANGEKQFANPRNAAAGSLR
                     QLDSQITAKRPLNFFAYAIARIEGWDQELESQTESLNQLHHWGFPICPENQVAQGVLG
                     CLAFYCVLMERRHSLPYQIDGIVYKVDNLALQRQLGFISRAPRFAIAHKFPAEEAITE
                     LLDVDFQVGRTGALTPVARLKPVFVGGVTVSNATLHNMDEIERKNIWIHDVVIVRRAG
                     DVIPEVASSVLERRPPHAKQIALPTHCPICGSDVIREAGEAAARCMGELGCSAQQKEA
                     ILHFSARRAMNIDGLGSKIVDQLVDAGLVKTIADIYKLNIDVLSRLERMGLKSAENLI
                     EAINKSKKTTFPRFLFALGIREVGETTAQVLANHFQDLEAIRVADEAVLQAVPDIGPV
                     VAAHIVMFFRQPDNLEVIQQLCAAGVYWSVKKLKSITEQPLKDHTFVLTGTLSSLTRE
                     DAKEQLQALGAKISESVSKKTNYVVAGESPGSKLAKAESLGIRILNEDQFLAILNKWQ
                     "
BASE COUNT          627 a          495 c          427 g          683 t
ORIGIN      
        1 agaataaatt aaaaagttag gaaacttata acagggttaa caatacaggc agaataacgc
       61 ctcactgcca cttatttaat atagccaaaa attgatcttc attcaggatc ctaatgccta
      121 aggattctgc tttagccaat ttagagccgg gagactctcc tgcaacaaca taatttgttt
      181 tttttgagac gctttcgctg attttggcgc ctaatgcttg taattgttct tttgcatcct
      241 ctcgagtcaa ggaacttaaa gtacctgtta atacaaaagt gtgatctttg agaggttgtt
      301 ctgttattga tttaagtttt tttactgacc aataaacacc ggcggcacat aattgttgaa
      361 taacttcaag attgtcgggc tgtctaaaaa acatcacaat atgtgcggcc accaccggcc
      421 ctatatccgg cactgcttgc aacactgctt catctgcgac ccgaatagct tctaaatcct
      481 ggaaatgatt cgccaaaacc tgtgcggttg tttctcccac ttctcgaatg cctaaggcaa
      541 ataaaaatcg tggaaaggtc gtttttttgc ttttgttaat tgcttcaatt aaattttcag
      601 cagactttag tcccatgcgt tccaatcgag ataagacatc gatattcagt ttgtatatat
      661 cagcaattgt tttaacaaga ccagcatcaa ccagttggtc tacaattttc gatccaaggc
      721 catcaatatt cattgcacgt ctggcagaaa aatgtaaaat agcttctttt tgctgtgcac
      781 tacaccccag ttcgcccatg caccgagcag ctgcctctcc tgcttctctg ataacatccg
      841 aaccacaaat agggcaatgt gttggcaacg cgatttgttt tgcatgtggc ggtcgacgtt
      901 caagaacgct agaagccact tcaggaataa catccccggc acgacgcaca ataacaacgt
      961 cgtgaatcca aatattttta cgttcaattt catccatgtt atggagggtt gcattactga
     1021 cggttacccc acctacaaac acaggtttta gtcgagcaac gggagtcaac gctcctgtgc
     1081 gtcccacttg aaagtcaaca tctaatagct cagtgatagc ctcttccgca gggaatttat
     1141 gcgcgattgc aaaacggggt gcacgtgaaa taaaaccgag ctgtcgttgc agagcaagat
     1201 tatcaacttt ataaacaatg ccatcaattt gatagggtaa tgaatgacgc cgttccatta
     1261 atacgcaata aaatgctaag caacccaaaa cgccctgtgc tacttgattt tctggacaaa
     1321 tcggaaaacc ccaatggtgt agctggttta aactttcggt ttgagattca agttcttgat
     1381 cccatccttc tatgcgcgca atagcataag caaaaaaatt aagaggtctt tttgcagtaa
     1441 tttgagaatc taattgtctt aagctccctg cggcagcatt acgcgggttt gcaaattgtt
     1501 tttcaccgtt tgcggttgca ttcttattca gcgcttcaaa acccgctttg ggcataaaaa
     1561 cctcaccgcg cacttctaat aagggtggca ctttcgttcc gtgtaatttt agcggaatgg
     1621 ctgaaattgt ccttaaattt tcggtaattt tttccccgct tactccatct ccccttgttg
     1681 ctcccatcat aaaaatacca ttttcatata acagtgttat tgctaagccg tccaacttag
     1741 gttcacaggt atactgaata acattttctg tttttaagcg cgcatgaatt cgtttatcaa
     1801 aatctcgcac ggtttcttct gaaaacgcat tatccaacga caacattggc acaacatgtg
     1861 ttacttcggg gaaatccttt aagggcgatg caccgacgcg ctgtgtcgga gaatccatgg
     1921 tttttaattc aggatgttga gattcaagct gttttagttc ttgaaataaa cgatcatatt
     1981 ctgaatctgg aatggtcggt gcgtctaaaa catgatagcg ataattgtgc tcgttaagtt
     2041 ccgatcgcaa ggcttttgca cgattaagtg ttttatcatc aatatcagtt ttaggattga
     2101 cttttttcat attctcgaaa agatactata taggtcaaaa gaggtcagac ttcccttggc
     2161 tttggcctca gagatttgac ccctctgtcc cctatagtat actgaacctt atggcaatac
     2221 ttccagcaga ac
//
LOCUS       DAORVT010000205         2228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_50323_length_2228_cov_3.089738, whole genome shotgun sequence.
ACCESSION   DAORVT010000205 DAORVT010000000
VERSION     DAORVT010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50323_length_2228_cov_3.089738"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..841
                     /locus_tag="PK389_04685"
     CDS             89..841
                     /locus_tag="PK389_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23033.1"
                     /translation="MKRIQFPFRFLFLLAVLLPYKTSYATCSPVEIEDSNKSNNYDTY
                     MGVNFKQNWIKPRGGWNNLFVKEQPGFNVYFGWRFHPNFGAELGYEWTDNKPKAFAVQ
                     PGSTLLGVLNNSGAPVTLTGKVRFKTGAADLNAFIPLCAEPLWEDVIPEGIISVGVAG
                     MKASMKIFSTTPATPFSNQFTTIQGRSKAVYRAGLGVQALIIENFGIRVLWRVESTSF
                     LRGRNSLTTTNNGTRVIFKNANTLAVGLFFKF"
     gene            complement(846..>2228)
                     /gene="gatB"
                     /locus_tag="PK389_04690"
     CDS             complement(846..>2228)
                     /gene="gatB"
                     /locus_tag="PK389_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HQY23034.1"
                     /translation="QSKLFSGAATAYGAEPNVQACAIDLGLPGVLPVLNTAAVRMAIK
                     FGLSIGSTIASRSIFARKNYFYPDLPKGYQISQFELPIVSGGSVEFTLPDGTPKKINI
                     TRAHLEEDAGKSLHEDFHGYTGIDLNRAGIPLIEIVSEPEMRSATEAVAYLKTIHALV
                     RYLEISDGNMQEGSFRCDANVSVRPLGQEKFGTRAEIKNVNSFRFVEKAINYEVKRQI
                     ELLENGGTVIQETRLYDANKDETRSMRAKEEANDYRYFPDPDLLPVIVDQTLIDEIRK
                     TLPELPREKATRFEKVYALSNYDAIVLTSSRELADFFEATVHTAKNSEAKLCANWIMG
                     ELLAALNKADLELTESPVSPEKLGRLVERITDKTISGKIAKVIFEEMWNTQKDVDLII
                     EEKSLKQVTDTAAIEVMIDAVIAANPTQVADYKSGKAQLFGFFVGQVMKASQGKANPD
                     QVNEILKGKL"
BASE COUNT          584 a          434 c          491 g          719 t
ORIGIN      
        1 ttaaaatcct ctataaaggg ggagtttaag gcttattgat tcctttgtgc taaaatgaac
       61 aataaagatt taagtaggga gttcaaggat gaagcgaatt caatttccat tcagattttt
      121 atttttgttg gccgttttac ttccctataa aacgagttat gcaacttgtt ctcccgttga
      181 aatagaagat tccaacaagt ctaataatta cgatacctac atgggtgtca attttaaaca
      241 gaactggata aagccgcggg gtgggtggaa taaccttttt gtgaaagagc agcccgggtt
      301 taatgtttat tttggttggc gttttcatcc taattttgga gcagagttag gatatgaatg
      361 gacagacaat aaacccaaag cgtttgcagt gcaacccggc agtacgttgt tgggtgtttt
      421 aaataattcg ggagcaccgg ttacgctgac aggcaaagtg cgctttaaaa cgggtgcagc
      481 agatttgaat gcgtttattc cgttgtgtgc ggagcctctc tgggaggacg ttattccaga
      541 agggattatt tcggtcggtg ttgcagggat gaaagctagc atgaagattt tttcgacgac
      601 acctgcgact ccatttagta atcaatttac gacgattcaa ggtagaagca aggccgttta
      661 tcgtgcgggt ttgggcgttc aagcattgat tatagaaaac tttggtatac gtgtattatg
      721 gcgtgttgaa agcacctcgt ttttacgggg gagaaatagc ctgacaacga ccaataatgg
      781 tacgcgcgtt atttttaaaa atgcgaatac gttggcggtg gggttgtttt ttaagttttg
      841 ataatttaca acttcccctt caatatctca ttcacctgat ccggattcgc tttcccttgg
      901 ctggctttca tcacttgtcc cacaaagaac ccaaataatt gcgctttgcc acttttgtaa
      961 tcggcaactt gcgttggatt agctgcaatc accgcatcaa tcattacttc gatcgcagcc
     1021 gtatcagtta cttgttttaa gcttttttct tcgataatca gatcgacgtc tttttgagtg
     1081 ttccacatct cttcaaaaat aacttttgca attttgcctg atatggtttt atccgtgatc
     1141 cgttctacaa gtcgtcccaa tttttctggc gaaacggggc tttctgtaag ctctagatct
     1201 gctttgttaa gtgcagctaa gagttctccc ataatccaat tcgcgcataa tttagcttca
     1261 ctgtttttgg cggtgtggac tgttgcctca aaaaaatcgg cgagttcgcg tgagctggtc
     1321 agtacaatcg catcataatt gcttaaggca tagacttttt caaaacgtgt ggctttttct
     1381 cgaggtaatt ctggtaaggt ttttcgaatt tcatcaatca gtgtctgatc gactatgaca
     1441 ggcagtagat ccggatctgg gaaataacga taatcattgg cttcttcttt agcacgcatc
     1501 gagcgagttt catctttgtt ggcatcatat aatcgggttt cttggataac tgttccacca
     1561 ttttctaaca attcaatttg tcgtttcact tcgtaattaa tggctttttc tacaaatcga
     1621 aaagaattca catttttaat ttctgcacgg gttccaaatt tttcttggcc aaggggtctt
     1681 acagaaacat tcgcatcgca tctaaatgaa ccttcttgca tattgccatc ggaaatctct
     1741 agatatcgca ctaaggcatg aatggtttta agataggcga cggcctctgt tgccgatcgc
     1801 atttcgggtt cagatacaat ttcaattaga gggatgccgg cgcgattgag atcgattccg
     1861 gtgtatccgt gaaagtcttc atgtaacgat ttgccagcat cttcttccaa atgcgcgcga
     1921 gttatgttta tttttttggg tgtaccatcg ggtagtgtaa attcaacaga accgccactc
     1981 acgattggta attcaaattg gctaatttga tagcctttgg gtaaatcagg gtaaaaataa
     2041 ttttttcgag caaaaataga tcgtgacgca atggtcgatc caatacttaa accaaattta
     2101 attgccatgc gtacggcggc tgtatttaat accggtaaca ctcccggtaa acctaagtca
     2161 atggcgcagg cttgaacatt gggttcggcg ccgtaggcag tagctgcccc tgaaaacaat
     2221 ttagattg
//
LOCUS       DAORVT010000206         2193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_51799_length_2193_cov_2.960711, whole genome shotgun sequence.
ACCESSION   DAORVT010000206 DAORVT010000000
VERSION     DAORVT010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51799_length_2193_cov_2.960711"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..837)
                     /locus_tag="PK389_04695"
     CDS             complement(10..837)
                     /locus_tag="PK389_04695"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010135006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HQY23035.1"
                     /translation="MDWITVKEYEDITYKKCNGVARIAFNRPNVRNAFRPKTTSELYQ
                     AFYDAQEDTSIGVVLLSAEGPSTKDGVYSFCSGGDQNARGHQGYVGDDGQHRLNILEV
                     QRLIRFMPKVVIAVVPGWAVGGGHSLHVVCDLTLASKEHAIFKQTDADVTSFDAGYGS
                     AYLAKMVGQKKAREIFFLGRNYSAQEAMEMGMVNAVIPHAELEDTAYQWAQEIMEKSP
                     TSIKMLKFAMNLTDDGMVGQQVFAGEATRLAYMTDEAKEGRNAFLEKRKPNWNLSAK"
     gene            1325..2179
                     /locus_tag="PK389_04700"
     CDS             1325..2179
                     /locus_tag="PK389_04700"
                     /inference="COORDINATES: protein motif:HMM:NF024484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HQY23036.1"
                     /translation="MTLKKIFFYSLFLFIICPFSYAQHLDSSSLETPISLNSIDSTTV
                     ISEETQIILKDSVTPKKDSIVYLSIKDSIVYPPPYRDWRRLGITSSMYVGATVIAFGV
                     LWVMPESTTNWDKEEMKEKGIAWKWKENVKAGPVWDEDNWVLNWITHPYSGGVYYMTS
                     RSAGFSRLESFGYSAIMSTFFWEYGIEAFAEIPSIQDLIITPVVGSVIGEGFFYAKKS
                     ILKHDKKVLKSRTLGITTLFLIDPFNTILDGLNYKEKVKTQMNLAPVGVNQFSKTPIW
                     GVQFSASF"
BASE COUNT          639 a          430 c          438 g          686 t
ORIGIN      
        1 atgattgact cacttcgcag atagattcca gttgggtttt cgtttttcta aaaaagcatt
       61 acgcccttcc ttagcttcat cagtcatata agctaaacgt gtagcttcac ctgcaaaaac
      121 ttgttgtccc accattccgt catcggtaag attcatagcg aattttagca ttttgatgga
      181 ggttggtgat ttttccatta tctcttgtgc ccattggtag gcggtgtctt cgagttctgc
      241 gtgggggatt acggcattga ccattcccat ctccatcgct tcctgagcgg aataattgcg
      301 tccgaggaag aagatttcac gggctttttt ctgtcccacc attttggcca aataagcgga
      361 accgtaacca gcgtcaaaac tggtgacatc ggcatcggtt tgtttgaaga tggcgtgttc
      421 tttactggcc aaagtcaaat cacatactac gtgcaaactg tgtcctccac ccactgccca
      481 accgggaact acagcgataa ctacctttgg cataaagcga atcagtcgtt gcacttcgag
      541 aatgttcaag cggtgttgcc catcgtcacc tacatagcct tggtgacctc gggcattttg
      601 atcgccacca ctacagaaag aatacacccc gtctttggtc gatggtcctt cggcggaaag
      661 caacactacg cctatcgaag tatcttcttg agcatcataa aaagcttggt ataattctga
      721 tgtggtttta ggtcgaaaag cattgcgaac attgggtctg ttgaaggcta ttcgcgccac
      781 gccattgcat tttttatagg tgatgtcttc gtactctttg acggttatcc aatccattat
      841 tgtcgtttta aattataaat gtaaaaatag cgcatttttt atatttttct aataattctc
      901 aaattaaata acaagtccta ttgtgagcaa cattattgag acgcttacag cgtgacaaat
      961 agtaagaatg attgagtcat aaagaactgt tgaattgtga gctacataat tgagactctt
     1021 ccagcgtgac aaatagcaag aaaaagaatt ggtacaatat tgatgttaaa taaacgaggg
     1081 ctactccttt tgtctcgctg gaagcgactc ttggctcgag agttcaaggg cagtggctaa
     1141 aattttaaat gcaaataatc tgttcgttat gcgaaatata ttttttaact atatttgcag
     1201 ccgttacgta caaaacattt gttaagtcag aagattcaat tttctgatta tcaaaataat
     1261 acaaattaaa tacaccgatt tgacttgttt aaaaagtgtt ttttatttca attatttgct
     1321 aactatgact cttaaaaaga tcttttttta tagcctgttc ctatttatta tttgcccatt
     1381 ctcttatgcg caacacttgg atagcagcag tttagaaact ccaattagcc taaacagtat
     1441 tgattccaca acggttattt cagaggaaac ccaaataatt ctcaaagaca gcgttactcc
     1501 aaaaaaagat tctattgttt acctgtctat taaagactcc atagtttacc cacctcctta
     1561 tcgggattgg agaagattag gcataacatc ttcaatgtat gttggggcaa cagtaattgc
     1621 atttggagta ctttgggtga tgcccgaaag caccaccaac tgggacaagg aagagatgaa
     1681 agaaaaaggg attgcttgga aatggaaaga aaacgtcaaa gcaggaccgg tttgggacga
     1741 agacaattgg gttttaaatt ggattacaca tccgtatagc gggggagttt actatatgac
     1801 ttcccgaagt gcaggattta gcaggttaga atcttttggt tattcggcta ttatgtccac
     1861 cttcttttgg gaatatggca tcgaagcctt tgccgaaatc ccttctattc aagaccttat
     1921 tatcacaccg gttgtgggtt cggtaatagg agaaggattt ttttatgcca agaaatcgat
     1981 tctaaaacac gacaaaaagg ttttaaaatc aagaacttta ggcataacta ccttgttttt
     2041 gatagatcct ttcaatacta ttttggacgg cttgaattac aaagaaaaag tcaaaaccca
     2101 aatgaatttg gctcctgtgg gtgtcaatca attctccaaa actcctattt ggggggtaca
     2161 gttttcggct agtttctaaa gggtttaccc cct
//
LOCUS       DAORVT010000207         2185 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_52145_length_2185_cov_3.825822, whole genome shotgun sequence.
ACCESSION   DAORVT010000207 DAORVT010000000
VERSION     DAORVT010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52145_length_2185_cov_3.825822"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..592
                     /locus_tag="PK389_04705"
     CDS             83..592
                     /locus_tag="PK389_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23037.1"
                     /translation="MEHISELTDILNGYLGWNKARIGCFVNMLLALISTRTVNLNKLA
                     CVVNGNTQQPSRYRRLQRFFAGYLINYDQIAGFIFKIFFIQGGSWYLTMDRTNWRWGA
                     ADINVLTLGIAYKGAAIPIYWELLDKRGNSDTSERIALMQKFIDQFGSMKNRVGNQIL
                     TFRIKNKSY"
     gene            complement(531..1637)
                     /locus_tag="PK389_04710"
     CDS             complement(531..1637)
                     /locus_tag="PK389_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HQY23038.1"
                     /translation="MNSEQLFKMALGIENPWEVVGVSFKEQSGEAAKELHIDIDFKRG
                     AKFPDPLGKLCSVYDTKQKTWRHLNFFQHPCYLHCRVPRIECSDGQIKLVEVPWSRPG
                     SGFTLLFEAYAMALIAGEMPINKVGKLLNEDAHRIWTIFNHWIGRAYASDKLNVPEKL
                     GFDETSSKKGHEYVTVAVDLDEKRVIHVAEGKDIKAVATVKDYLESKAIDVNRVKHAS
                     MDMSPSFISGAMKHFPKAEIHFDRFHVVKLLNEAMNKVRISERKEHDELKGHKYTFLK
                     NSQSLSDKRKKELSSLIKLFPIKEFTKTVKAHWTGIVNFCETQINNGILEGINNKIQL
                     AKRRARGYRCMENFINMIYFLCGKLKFDYPLYSS"
     gene            1691..2185
                     /locus_tag="PK389_04715"
                     /pseudo
     CDS             1691..2185
                     /locus_tag="PK389_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212682.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          631 a          428 c          426 g          700 t
ORIGIN      
        1 cctctgtaca cgacaatttt tgatttgaaa cgtaactcgc tgataaaaca agatataatc
       61 tcaatgatga gaaggaggtt atatggagca tatcagcgaa ttaacagata tcctaaacgg
      121 atatttgggt tggaacaagg cgcgaatagg ttgttttgta aacatgctat tggcacttat
      181 ctcaactcga acggtcaact taaacaaatt ggcttgtgta gtaaatggca acactcaaca
      241 gccttcaaga tatcgtcgtt tgcagcgatt ttttgcaggc taccttataa attatgacca
      301 aatagcgggt tttattttta agattttttt tattcaaggc ggttcttggt atctaaccat
      361 ggatagaacc aattggcgct ggggtgcggc cgatattaac gtgctaactt taggtattgc
      421 ttataaaggg gcggccatcc cgatttactg ggagctattg gataaacgag gcaattcgga
      481 tacctctgaa cgtattgcct taatgcagaa attcattgat caatttggct ctatgaagaa
      541 tagagtgggt aatcaaattt taactttccg cataaaaaat aaatcatatt gataaagttt
      601 tccatgcagc gataaccacg tgctcttctc ttcgccagtt gaatcttatt gttgattcct
      661 tctaaaattc cattgttaat ttgcgtttcg cagaaattaa caatgcctgt ccaatgagct
      721 ttgaccgttt ttgtaaattc tttgatcgga aataatttaa tcaaactgct aagctctttc
      781 ttgcgcttat ctgataagga ctgagaattt tttaaaaagg tatatttgtg tccttttaat
      841 tcatcatgtt cttttctttc agaaatgcgt actttattca ttgcctcatt taaaagtttc
      901 acaacatgaa atcggtcaaa gtgaatctct gcttttggaa aatgtttcat ggcacccgaa
      961 ataaaactcg gtgacatatc catgcttgca tgtttaacac ggttcacatc tattgctttc
     1021 gattctaaat aatccttgac tgtagcgact gctttaatat ccttgccttc tgcgacatga
     1081 atcactcttt tctcatctag atctaccgcc actgtcacgt attcatgtcc ttttttgcta
     1141 gaagtttcat caaaccctaa tttctctggc acattaagct tatctgatgc gtaagcccgt
     1201 cctatccagt gattaaaaat tgtccatatc cgatgcgcat cttcatttaa taattttcct
     1261 accttattaa ttggcatttc acctgcaatc aatgccattg cataagcttc aaataaaagt
     1321 gtaaaaccac tgccagggcg tgaccatggc acttcaacta gttttatttg gccatcagag
     1381 cactcaatgc gtggcacccg acaatgcagg taacaaggat gttggaaaaa attgaggtga
     1441 cgccatgttt tctgtttggt gtcgtaaacg ctgcaaagtt taccaagcgg atctgggaat
     1501 ttagcacctc gtttgaaatc gatatcaata tgcagctcct tagcagcttc acctgattgc
     1561 tctttaaaac taacacctac aacttcccat ggattttcta tccccaacgc catcttaaat
     1621 aattgttcgc tattcattca caaccccatc attaaagctt gaatccgata gcgcttataa
     1681 taagtcaccc attcaaaaat tcatagaacc atcaatttgg caaagactgt attaaaggcg
     1741 tattagcaga tagggaattc attgggaaag actggtttgc ttggcttctt aaagcagcta
     1801 tttcgtttga tatacgaatt aaaagtaata ctgttaccac gaatgctcga ggtctttcag
     1861 tagatattga tggtttgttt tatgatctca atgcaggaga acaacgctcc ttgcaaggac
     1921 ggcgcaaagt gtggggacac ttgctttatt tggcaggatt gcggttagag gacggagatt
     1981 tattgattgt tgcaacccct gaagagccag aaaatgccat aaaaacctat gggttgaggt
     2041 gggaaataga aactttgttt ggctgtttaa aagggcgcgg gttccatttt gaggacaccc
     2101 atattacagg acgggaacgc attaaaaaac tgatggtttt gcttgcgatt gctttttcat
     2161 gggcgcatcg cacaggagaa tggag
//
LOCUS       DAORVT010000208         2170 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_52809_length_2170_cov_2.822222, whole genome shotgun sequence.
ACCESSION   DAORVT010000208 DAORVT010000000
VERSION     DAORVT010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52809_length_2170_cov_2.822222"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..1455
                     /locus_tag="PK389_04720"
     CDS             4..1455
                     /locus_tag="PK389_04720"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HQY23039.1"
                     /translation="MIELDFSKPAPKPSSIEEGHLVIDALWTFCGNLHKELALLKQEV
                     IAFKKENAELKERLNTNSNNSSKPPSTDSKKNNKKKNYKGSGRHPGGQPGHKGVFRKL
                     VPIEQVDAVITCPPPDKCQDCNVSLLPLKEIVRHQVYEIPLPRYEITEYQILQGLCSC
                     CQKTYRGETPAKVGRRGFGVRVHSSIALLTSKFKLSKRQALELLKDFFQMPICVGSVS
                     NIEHRVSQSLEPLHTHIKAQIDRSKVAHIDETGFKQSNHSGWAWILANELFSFLQLDH
                     SRGKKVAKKLIGSFMNRIIVSDRYPAYDFLPELSHQVCWAHLKRDFQKISERSGIAGV
                     IGRRLLHGYGKIFAFWKSNLQQAIDDKRTRKKRRQLKNRFLKDLEFGASCSHLTTART
                     CRNILDMGKSLWLFLENRDVPATNNLAERQIRPLVIAKKLSFGVKSDRGARFIESAYS
                     LVLSCRQQNKNALSWLQDSITSHFISESPPLLI"
     gene            1482..1694
                     /locus_tag="PK389_04725"
     CDS             1482..1694
                     /locus_tag="PK389_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY23040.1"
                     /translation="MNPARQRLIYTDQLWVHPDHRKKALGHQLMEAVHDYGRKSGCTM
                     ATVATMNFQGAKAFYEKYPRYALKHL"
     gene            1904..>2170
                     /locus_tag="PK389_04730"
     CDS             1904..>2170
                     /locus_tag="PK389_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQY23041.1"
                     /translation="MSVIIGRKAEQKILLNVMQSTAAEFVVVYGRRRIGKTYLIEQFF
                     LKHDCLFFRVTGIQNGKIKDQLREFAKGIGLSFYQDTVLTVNPIN"
BASE COUNT          696 a          401 c          466 g          607 t
ORIGIN      
        1 taaatgatag agttagattt ctcaaagcca gccccaaaac cttcttcgat tgaagaaggt
       61 catttagtta ttgatgctct atggactttt tgtggcaatc ttcataaaga gctggcactt
      121 ctcaaacaag aagttatagc ctttaaaaag gaaaatgcag agctgaaaga aagactcaat
      181 acaaactcta ataactcgtc caaaccacca tcaacagata gtaaaaagaa taacaagaaa
      241 aaaaattaca aaggcagcgg tcgtcatcct ggaggtcaac ctggacataa aggggtgttt
      301 cgcaaattag tgcccataga gcaagttgat gcagtaataa cgtgtccccc accggataaa
      361 tgccaagatt gcaatgtttc cttgctgccc ttaaaagaaa ttgttaggca ccaggtttat
      421 gaaatacctt tacctcggta tgaaataacc gaatatcaaa ttttgcaggg attatgtagt
      481 tgttgccaaa aaacctacag gggggaaacg cctgcgaaag ttggaagaag aggatttggg
      541 gtgcgagtgc atagtagtat agcgctgctg acttcaaaat ttaagttgag taaacggcaa
      601 gcgttagaac tgttaaaaga tttctttcaa atgccgattt gtgtaggcag cgtttcaaat
      661 attgagcatc gagttagcca atccctagag cctttgcata cacatattaa ggcccaaata
      721 gatcgctcaa aagtagccca cattgatgaa actggattta agcaaagtaa tcattctggt
      781 tgggcttgga tactggcgaa tgaattattt agctttttgc aattagatca ttccaggggg
      841 aaaaaggttg ccaaaaaatt aattggtagc tttatgaacc gaattattgt ttcggataga
      901 taccctgctt atgattttct gccagaattg tcgcatcaag tctgttgggc tcacttaaag
      961 agggactttc aaaagatctc agaacggtcg gggatagcag gtgttatagg caggcgatta
     1021 ttacacggtt atggaaaaat ttttgctttt tggaagtcta atttacagca ggctattgat
     1081 gataaacgta cacgaaaaaa gcgccggcag ttaaaaaata ggtttctgaa agatttggaa
     1141 tttggtgcaa gttgcagcca ccttactact gcccgtactt gtcgcaatat cttggatatg
     1201 ggtaaaagtt tatggttatt cttggaaaat cgcgacgtgc ctgccacgaa taatctagcg
     1261 gaacgtcaga ttcgcccgtt ggtgattgct aagaaattaa gctttggcgt gaaatcagat
     1321 cgcggggcaa gatttataga gagtgcctac agtttagtat tgagttgtcg acaacaaaat
     1381 aagaatgcgc tttcctggct tcaagattct ataaccagcc actttatatc ggaatcccca
     1441 ccgcttctta tataaaattc gttgcaggta tcaataagca tatcaaccct gcgcggcagc
     1501 gcctcattta taccgatcaa ttgtgggtac atcccgatca tcgcaagaaa gctttaggac
     1561 atcaattgat ggaagcagta catgactatg gccgcaagtc aggatgtacc atggcaaccg
     1621 ttgcaactat gaattttcag ggtgccaaag cattctatga aaaataccct cgttacgcct
     1681 taaaacactt gtgacaaagc gacttgaagt gctcttgcag cacaagaaca gaagtgattt
     1741 agatgattaa ggatagagca gctacaccta taagtcactt aagccactgc atacgcggtg
     1801 ggctggatat agactattta tatccagccc atcgattgct ttggtgggct ggatatatta
     1861 tacttatact cagcccatct aaattaacgg agaatttcta tcgatgagtg tcattatagg
     1921 ccgcaaagct gaacaaaaaa ttttgttaaa tgtaatgcaa tctaccgctg ctgaattcgt
     1981 tgttgtgtat ggtcggagac gaattggaaa aacatacttg atagaacaat ttttcttaaa
     2041 gcacgattgt ttattttttc gcgttacagg catacaaaat ggaaaaatta aagatcaatt
     2101 acgtgaattt gcaaaaggaa taggacttag tttttatcaa gataccgtct tgactgtcaa
     2161 ccccataaat
//
LOCUS       DAORVT010000209         2160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_53209_length_2160_cov_3.739192, whole genome shotgun sequence.
ACCESSION   DAORVT010000209 DAORVT010000000
VERSION     DAORVT010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53209_length_2160_cov_3.739192"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PK389_04735"
     CDS             <1..201
                     /locus_tag="PK389_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HQY23042.1"
                     /translation="FAGCVLVISHDRWFLDRIATHILAAEGESQWAFYNGNYQEYEAD
                     KKKRLGEEGAKPKRIRYKPISR"
     gene            266..1378
                     /locus_tag="PK389_04740"
     CDS             266..1378
                     /locus_tag="PK389_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQY23043.1"
                     /translation="MSTKNSIHKLTIRGFKSIKHLDEFYLDQLNILIGANGAGKSNFV
                     SYFRMLNEMVESRLQSWTRNQGSADRIVSFGIKETDKVESFVEFGLNGYKFALEPTVD
                     GGFSFYEEKLFFDGPYYGKKWIPLGSGHTEAKLKETFKTAKPGGVADYCYNSIANWKV
                     FHFHDTSDTAGVKRLGSLHDNEYLRPDASNLAAFLYRLSGEYEDVYKQIQKTICLAIP
                     FFDDFVLKPQKLPSEEEQIRLLWRQKDSDYSLWPSQLSDGSIRFICLVTALLQPNPPS
                     TIIIDEPELGLHPYAITLLGSLLRSASNRMQVIISTQSVPLVNEFSIADLIIVEREQG
                     GTVFKRFDEEDFKTWLEDYSVGELWEKNILGGRPTK"
     gene            1379..2053
                     /locus_tag="PK389_04745"
     CDS             1379..2053
                     /locus_tag="PK389_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HQY23044.1"
                     /translation="MIRVHVICEGQTEEMFVKEILSGFFSSRGICLIPTLIGKPGHKG
                     GNFKFDRLFTDVRARLLGDKTSYCTTFFDFYGLPENFPGKQEALGCLTLQKKAQRVLD
                     VMSDELREKLGEESLRRFIPYIQMYEFESLLFSAPDVLAQAIYKPDLKLEFQKIRDKF
                     TTPEEINNSPLCAPSKRIKKLYSGYDKPIHGSLAAIEIGLNTIRSECHIFDSWLNRIE
                     KLQGIN"
BASE COUNT          659 a          398 c          468 g          635 t
ORIGIN      
        1 tttgccggtt gcgtactggt catctcgcac gaccgctggt tcctcgaccg tatcgccacc
       61 cacatcctcg ccgctgaagg tgaatcacaa tgggcgttct acaacggcaa ttaccaggaa
      121 tacgaagccg acaagaaaaa acgcttgggt gaagaagggg ctaagccgaa acggatacgg
      181 tataagccga ttagcaggta aaaccttaga tgccaaagtc atgccgtgta ttgattcaat
      241 atttaatttt cggactcttt aaataatgag cacgaaaaat agcattcata aattgacaat
      301 tagaggattt aagtcgatta aacatcttga tgagttttat ttagatcaac ttaatatact
      361 gatcggtgct aatggggctg gaaaaagtaa ttttgtttcg tattttcgta tgctgaatga
      421 aatggttgag tcacgccttc agagttggac tcgaaatcaa ggatctgccg atcgcattgt
      481 tagctttgga ataaaggaaa cagataaagt tgagtctttt gttgagttcg gactcaatgg
      541 ctataaattt gcacttgagc caacagtaga tggcggcttt tctttttatg aagaaaagct
      601 tttctttgat ggtccatatt atggcaaaaa atggattcca cttggctctg ggcataccga
      661 ggctaagcta aaagaaactt ttaaaacagc aaaaccgggt ggcgttgcag attattgtta
      721 taactcgatt gccaattgga aggtatttca ttttcatgat actagcgata cggctggtgt
      781 aaaaaggcta ggttctctcc atgataatga atatcttcgg ccagatgctt ctaatttggc
      841 tgcattttta tatcgattgt ccggcgaata tgaggatgtt tataagcaaa ttcagaaaac
      901 aatatgttta gcaataccat tctttgatga ttttgtttta aagccacaga aactgccatc
      961 tgaagaagaa caaattagat tgctttggag gcaaaaagac agtgactatt ctttgtggcc
     1021 tagtcaactc tcggatggat caattcgctt catttgctta gttactgctt tgctgcagcc
     1081 caaccctcca tcgaccatta ttatcgatga gccagaactt ggcttgcatc cctatgcgat
     1141 taccttgctt ggttcgttgc tacgttcagc atctaatcgc atgcaagtaa ttatctctac
     1201 acaatcggtt ccattagtga atgaattttc gattgccgat ttgataattg tagaacgtga
     1261 acaaggtgga actgttttta aacgatttga tgaggaggat tttaaaactt ggttggaaga
     1321 ctattcagtc ggtgagctat gggaaaagaa cattctgggc gggcggccaa ccaagtaaat
     1381 gatacgcgta catgtaattt gcgaagggca gacggaagaa atgtttgtta aggaaatact
     1441 ctcaggcttt ttttcttcac gaggtatttg cttgattccg acattgatcg ggaaacctgg
     1501 gcataagggc ggcaacttta aatttgacag actatttact gatgtgcgag cccggttatt
     1561 aggtgacaaa acatcgtact gcacaacttt ctttgatttc tatggattgc cagaaaattt
     1621 tccagggaag caagaagctc tgggctgctt aacccttcaa aaaaaagcgc agcgcgtgct
     1681 agacgttatg tcagatgaat taagggaaaa gctgggcgag gaatcactga gacgttttat
     1741 tccatacatc caaatgtatg agtttgagag tttgctcttt agtgcgccag atgtgttggc
     1801 gcaggcaata tataaacccg acttaaagtt agagtttcaa aaaattcgcg ataaatttac
     1861 tacccctgaa gagataaata atagtccact ctgcgcgcca agtaagcgaa ttaaaaagtt
     1921 atattctgga tacgataaac caattcatgg ctcacttgct gcaatagaaa ttgggcttaa
     1981 tactattcgt tcagaatgtc atatatttga tagttggcta aatagaatcg agaaattaca
     2041 gggaatcaat taacaagact catattaggt cgacgaaaat ctgaactatg taccacttta
     2101 atacttcact aaattgaacg acatccaaca actcaccaaa attttgcgca aattccgcga
//
LOCUS       DAORVT010000210         2154 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_53502_length_2154_cov_2.301096, whole genome shotgun sequence.
ACCESSION   DAORVT010000210 DAORVT010000000
VERSION     DAORVT010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53502_length_2154_cov_2.301096"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(322..819)
                     /locus_tag="PK389_04750"
                     /pseudo
     CDS             complement(322..819)
                     /locus_tag="PK389_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1157..1588
                     /locus_tag="PK389_04755"
     CDS             1157..1588
                     /locus_tag="PK389_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008739793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQY23045.1"
                     /translation="MAKKLSKTASKKPVPKSKAAEPKKTVVAKKVAVPKVPKLLSPKT
                     PYKKTELFATLTAQTGLNKKEVQKVLETLQTLMRLHLVKNGPGQFVLPGVFKMTAITK
                     PATKPRTGRNPFTGEEMMFKAKPARRVVKVRILKKFKADIE"
BASE COUNT          674 a          415 c          385 g          680 t
ORIGIN      
        1 aattttcttg gctttaaagc agaaatagtt actttctttt cgccagctat tactttttct
       61 tccaaaagat aaaccgttaa ttccgtatca gatgtatcag aacacccgca tgctattgtc
      121 atggaaataa taatggctaa cgtcctaaaa atattaattt taatttactc ctaataattg
      181 atatctttta tacattgacg agttgaacca tcgctcgcct aactagaaac cagataattg
      241 cttcggaacc aatgttatgc ggcaagtcta tgagtgctat taggccttga tctggttttt
      301 gctaataatc gtatcagagg taattatttc tggcaatgct ttacaaattt tattaatgcc
      361 ttgtggaacc acaatgccaa actctgctaa taaaccacgt atttgattga ccaaggctat
      421 tctttctgca accaatcggc ttctaactct atgtaccgct tgaatacctt cttgttccac
      481 gcttttaggc gagacataac gcatgctagg ccgagaagcc gcttcacaaa tagcttctga
      541 gtcatttgcg tcatttttat tagtttttac gaaaggtttt acaaattgtg ggctaataag
      601 tttgacctca tgtcccattg ctttaaattt tctacaccaa taatttgcct cactacaggc
      661 ttccatcaca atcgtacacg tttgtaattt tacaataaat tccatcaatt tgcttctagt
      721 aagtcgtttt cttaacacag gatttctttt atcgtccaca ccatgtaatt gaaaaacatt
      781 ttttgcaaga tcaatgccta ataatgtaac attttttatg gaccttctcc ttacctctat
      841 ggtttaaatg gttgattcat caccattttg gctcttttag aagcggttta aaaggggtgg
      901 gtccattcca ttgtccgcgg cgtaaatata gccgtcgcgc ctccgagtat attttggcga
      961 cttgtcccaa aatttttcac gaggtgcgcg acacccggga gcgtttatat atggctgaca
     1021 tacttaacaa aataatctaa atttcgtaat attaaaaaaa ttaaattttt ggcttgcttc
     1081 atgcttatta ttttagttca cagattgaaa ttagtgcgtt tatcgtgtat aagaaagttt
     1141 taaggagaaa caaattatgg caaaaaaact gagtaaaact gcttctaaaa aaccagtacc
     1201 gaagagcaag gccgcagagc ctaaaaaaac agttgtcgct aagaaagtag ctgttcccaa
     1261 ggtgccgaag cttctgtctc caaaaactcc ctataagaaa acagaattgt ttgcgacgct
     1321 aaccgctcag acaggtctta ataaaaaaga ggtacaaaaa gtcttagaga ccttgcaaac
     1381 tctgatgagg cttcatttag ttaaaaacgg cccaggccaa tttgtattgc cgggggtatt
     1441 taagatgacg gccattacta aacccgcaac caagcctcgg acgggcagaa atcctttcac
     1501 cggcgaggaa atgatgttta aagctaaacc ggcaagaaga gtggttaaag tccgcatctt
     1561 aaagaagttc aaagctgata ttgaataaaa ggcgcttgac tgaatttgcc tgccgctagt
     1621 cagtggagag agcgctttgc tctctccatt cttctccgag cctatgaaac aaaaaaacag
     1681 ggattagcgt catttaaacc cagagcaatt gtgcaatctg aagtaagcaa ctgtgataca
     1741 agatcagaat ccagggtgta gttaacggct taaattctgt tcagttttgg ggtattcaac
     1801 cctaattttt gtcttacgat cccatagaaa atttggtaaa agctctaata acttatagtg
     1861 attccccgaa aaatgaatga cgctttaaag tgccgttctt aattttttct actcggcaaa
     1921 caaaattgtg tcaaaatatc gagtggggtc tcaataattt tttaaacagt attttttaaa
     1981 cagtgaagcc taataaatga aaaaatactg atttgagctg atcagcttag gtgtgtagag
     2041 agaatctcct acgagatatt tgtataaaat atggatgatt ttaaaatgga aaaaaaacgc
     2101 tttaaatctc taattcaatt ctgcatactc cttatagtat tttttagcat tcca
//
LOCUS       DAORVT010000211         2140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_54120_length_2140_cov_3.222062, whole genome shotgun sequence.
ACCESSION   DAORVT010000211 DAORVT010000000
VERSION     DAORVT010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54120_length_2140_cov_3.222062"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PK389_04760"
     CDS             <1..410
                     /locus_tag="PK389_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HQY23046.1"
                     /translation="LIVHTRNARKDTLAILKAGHANECGGVLHCFTEDWDTASAALDL
                     DFYISFSGIVTFKNAIDLQAVAKKLPLERILIETDSPWLAPMPYRGKINQPAYVKHVA
                     EFLADLRGVTFEEIAKHTTQNFYRLFAQTRIES"
     gene            480..680
                     /locus_tag="PK389_04765"
     CDS             480..680
                     /locus_tag="PK389_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23047.1"
                     /translation="MKNAALYTSGILFCVIAGAQFLRYKLGVAIMVGSKLQIPTELSL
                     YAAVILLVFGLWMFIAALTKNH"
     gene            complement(768..1253)
                     /locus_tag="PK389_04770"
     CDS             complement(768..1253)
                     /locus_tag="PK389_04770"
                     /inference="COORDINATES: protein motif:HMM:NF028308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HQY23048.1"
                     /translation="MLKLKRLIGSLLLVFLIAGCAGAGPKQTGGQLLGGAAGALLGSQ
                     FGKGSGRLFGVAIGALAGSYLGGSLGHAMDEKDRQMAQSTMLNTLESAPDHQTRSWKN
                     PNNNHAGTVAVTRTQEVKQSNMVCRDYVHTVIIDGQQEKMHGRACRDVRDPKGHWQVE
                     N"
     gene            complement(1402..>2140)
                     /locus_tag="PK389_04775"
     CDS             complement(1402..>2140)
                     /locus_tag="PK389_04775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HQY23049.1"
                     /translation="AAALIISITAMRLGRRQADQKRTFGYYRIEILAAAFNAATLFMV
                     AGFIFYMAYERFFVSSEIHTTSMLIVASIGVVINFISTQLLKAGSVDSLNIKSAYLDS
                     QADMLSSLGVILTAFLIRLTAWQYLDSLMAIGISLWILPRTWILLKESINILLEGVPE
                     GIELAEISGALLDLPGVTDVHDLHVWALTNGKISLTAHIVIEPNAEKEHDLLAVATYL
                     LEEKFNVTHSTIQIETTQCHGNSKLHL"
BASE COUNT          649 a          461 c          415 g          615 t
ORIGIN      
        1 ctttaattgt gcatacgcga aacgctcgca aagatacact ggcaatttta aaagccgggc
       61 atgcaaatga atgtggtggt gtgctgcatt gctttacaga agattgggat acggcaagtg
      121 ctgcactcga tcttgatttt tatatttcat tttcggggat tgtaaccttt aaaaatgcta
      181 tcgatctaca agcggttgct aaaaaactgc ctttagaacg catcttaatc gaaacagact
      241 caccttggtt agcgcccatg ccttaccgcg gaaaaatcaa tcaacccgcg tatgttaagc
      301 acgttgccga atttttagca gatttgcggg gtgtgacatt tgaagaaatc gcaaaacaca
      361 caacccaaaa tttttatagg ctatttgcac aaacccgaat agagtcttaa gctatacttc
      421 ataaagtaaa cgaatttacg cttaattttt acagttaaaa acgatgagag gagaatgaca
      481 tgaaaaatgc agcattatat acatcaggta ttcttttttg tgtgattgca ggtgcacaat
      541 ttttaaggta taaattggga gtcgctatca tggtgggtag caagcttcag atcccaaccg
      601 agctatcgct atatgcagcc gttattctgc ttgtttttgg actctggatg tttatcgcgg
      661 ctttaacaaa aaaccattag agcctgaggc tgttatttta aaattttatc gttgaagaac
      721 tcaagggcgg agataacgat ccgcccctat cggtagttcc ttatccttta gttttcaacc
      781 tgccaatgtc ctttaggatc acgaacatct ctacaagcac gtccgtgcat tttttcttgt
      841 tgtccatcaa taataaccgt gtgaacataa tccctacaaa ccatgttact ctgcttcact
      901 tcttgcgtcc gtgttacagc aacagtaccc gcatggttat tatttggatt tttccaagaa
      961 cgcgtttgat gatcgggtgc tgattccaat gtatttagca tcgtgctttg cgccatttgc
     1021 ctgtcttttt catccatggc atgtccaaga gaaccgccca gataagaacc cgcaagcgca
     1081 ccaatcgcaa caccaaataa tctgcctgaa cctttaccaa attgggagcc cagcagggca
     1141 cccgcggcac cgcctagtaa ttgaccacct gtttgtttag gtccagcgcc tgcacaacca
     1201 gctattaaaa aaaccaacaa aagtgaacct atcaaccgct taagttttaa catataagct
     1261 cctaaaaaaa ttgttatatt tcggaaaaaa ggagtcagct ccctttgcgc tcccgacagc
     1321 cagataatat ggattttaaa attaaaagtc aattaatttt agttaattgc ccgagtcatg
     1381 tcttgagtga caatacactc ttcacaaatg taacttggaa tttccatgac attgtgtggt
     1441 ttcaatctga attgtagagt gcgttacatt aaatttttct tcaagtaaat aagtcgctac
     1501 agctaataaa tcatgctctt tttcggcatt gggttctata acgatatgag cagtaagact
     1561 aatttttcca ttggtcagtg cccaaacatg tagatcatgt acatcagtta cacccggcaa
     1621 atctaaaagt gcgccgctaa tttctgctaa ttcaatacct tcgggtacgc cttccaataa
     1681 aatattgatg ctttccttaa gtaatatcca cgtgcgaggc aatatccaaa gagagatgcc
     1741 aatggccatc agggagtcaa ggtattgcca tgccgttaga cgaataagaa aagctgttaa
     1801 aataaccccc aaagaactca gcatatcggc ttgggaatcg agataggcac ttttaatgtt
     1861 aaggctatcg acgctaccgg ctttaagtaa ttgtgtgcta ataaaattaa taacaacccc
     1921 tatagaggcc actattagca tactggtggt atggatttcg ctcgatacaa agaagcgttc
     1981 ataagccata tagaaaatga accctgcaac cataaacaat gtcgcggcat taaaggcggc
     2041 tgctaaaatt tcaatacgat agtagccaaa agtacgcttt tgatcagctt gccgcctgcc
     2101 caaacgcatt gccgtaattg aaataatcaa ggcagcggca
//
LOCUS       DAORVT010000212         2108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_55639_length_2108_cov_3.544082, whole genome shotgun sequence.
ACCESSION   DAORVT010000212 DAORVT010000000
VERSION     DAORVT010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55639_length_2108_cov_3.544082"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /gene="istA"
                     /locus_tag="PK389_04780"
     CDS             complement(<1..987)
                     /gene="istA"
                     /locus_tag="PK389_04780"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HQY23050.1"
                     /translation="MAAIRVTVEMQEQILELFRQGRSERAIAKIVNKNRRTVSRIIQR
                     GAVVKPGSLAADWVMLIDWEKVRLEASRGVQINILAKEHAEGKISYVQFWREYNKKYP
                     THPTVTMKLIHKPGEKCFFDYTEGINIINRETGELTKTWLMCGVMAMSSMTYGEFTLT
                     QKRDDLIRSMERSFRYFGGVTPYVTVDNQKAAVDHAHWYDPDVNPGFIDFANHWGFAV
                     IPARPYRPRDKGGNESNIGVIQRQFYQEVRDKNFYSLSELNLAFLEYLKKLNHAEMKD
                     WGVSRSDRFEGEKHLLKPCPLSNWETSEWKTPKVHADCHIQVLKKFYSVPYQH"
     gene            complement(1102..1734)
                     /locus_tag="PK389_04785"
     CDS             complement(1102..1734)
                     /locus_tag="PK389_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23051.1"
                     /translation="MRGYQCLKSIYLTIHNKELEPPVTPDLQAIFDQGNAVGAEARKI
                     YPHGVMIDNPAWDFFGALKKTRELLQNETEVIFEAAFEYKGCFARADTVVYSKESKRW
                     SIYEVKSTTKVKDEHYDDVGLQAWIMANSGLPIEKICIMHINNECRYPNLENLFTAVD
                     VTEELRNRHNEIPAKLNSIFKAIKSETVPDDRFHPKCTTQFHQSTPVNFM"
     gene            complement(1773..2033)
                     /locus_tag="PK389_04790"
                     /pseudo
     CDS             complement(1773..2033)
                     /locus_tag="PK389_04790"
                     /inference="COORDINATES: protein motif:HMM:NF024555.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
BASE COUNT          587 a          455 c          376 g          690 t
ORIGIN      
        1 atgctgataa ggtaccgaat aaaacttctt caagacctgt atgtggcaat ctgcatgcac
       61 cttcggagtt ttccattctg atgtttccca gttagacaaa gggcatggct ttaataaatg
      121 tttttcacct tcaaaacgat cacttcgact tacgccccag tccttcatct ctgcatgatt
      181 taattttttt aaatactcta aaaatgctaa atttaattca gataaagaat aaaaattctt
      241 atcacgcact tcttggtaaa attgccgctg gataacaccg atatttgatt cgttgccgcc
      301 tttgtcacgc ggccgataag gccttgccgg aatcaccgca aaaccccagt gatttgcaaa
      361 atctataaat ccaggattta catctggatc ataccagtgc gcatgatcaa ccgctgcttt
      421 ttgattatcg actgtcacat aaggtgtaac accaccaaaa tatctaaatg aacgctccat
      481 tgacctgatt aaatcatcgc gcttttgtgt cagcgtaaat tcaccataag tcattgaact
      541 catcgccatc actccgcaca ttaaccaggt ctttgtgagt tcaccagttt cccggttaat
      601 aatgttgatg ccctcagtgt aatcgaaaaa acatttctct cctggcttgt gaatgagttt
      661 cattgtcacg gttggatgag tcggatattt tttattatat tcgcgccaga actgaacata
      721 gctgatcttg ccctcggcat gttcttttgc taatatattt atctgcacac cacgactagc
      781 ttctagtcga accttctccc aatcaattaa cattacccag tctgccgcca gagaccctgg
      841 cttgaccact gctccacgtt gtataattcg cgacactgtt cgacggtttt tatttactat
      901 ctttgctatg gctcgctccg agcggccttg tctgaataat tctaaaattt gttcctgcat
      961 ctccactgtt acccttattg ctgccatttc cacctctgga aacaacagga ccatcgacct
     1021 ataaacttgt cgctgacgct ttcaggtaaa aacctcaaat cgaatataag tggtaccctt
     1081 tgatgaaatc aggtggtgca cttacatgaa attgactggt gtactttgat gaaattgggt
     1141 ggtgcacttc ggatgaaatc ggtcatctgg tactgtttct gattttatag ctttaaaaat
     1201 actatttaat tttgcaggta tttcattatg acgattgcga agttcttcag taacatcaac
     1261 agctgtaaac aggttttcaa gatttggata tcggcattca ttattgatgt gcatgatgca
     1321 aatcttttcg attggtaagc cagaatttgc cataatccat gcttgtaatc cgacatcatc
     1381 ataatgctca tctttaactt ttgttgttga tttaacttcg taaattgacc agcgctttga
     1441 ttctttagaa taaacgacgg tatcagcacg agcaaagcag cctttgtatt cgaacgcagc
     1501 ttcaaaaatg acttcagttt cattttgaag taattcacga gttttcttta acgctccaaa
     1561 aaaatcccaa gctggattat caatcatcac accatgtgga tatatttttc gagcttcggc
     1621 gccaactgca tttccttggt caaatatagc ctgtaaatca ggagtgacgg gaggttccag
     1681 ctctttattg tggatagtta aataaatgct tttgaggcac tgatagccac gcataaggcg
     1741 agttttactg aggagtttag ggattgcggt cattattcaa aataatcact atcaatagtc
     1801 ggaataacaa tgtttcgttt tactgagacg cctacgccag attcaatcat gagttcagcg
     1861 attctctgtc catcgattaa tactaatgta tccgacacgg tactagcata ttgaatcgct
     1921 gattcttggt atgatgaagt agttagaaat actcctttag tagcatgtcg gccctttaaa
     1981 gcgccaaaga actcttgaat ttgacgacta ttaattttgt tttctggttt atatcgtttt
     2041 gcttgaagtt atattttatc taaacccaat ttatctaagt gaacaatacc atcaattcca
     2101 aaatcgcc
//
LOCUS       DAORVT010000213         2100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_56031_length_2100_cov_3.200978, whole genome shotgun sequence.
ACCESSION   DAORVT010000213 DAORVT010000000
VERSION     DAORVT010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56031_length_2100_cov_3.200978"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..110
                     /locus_tag="PK389_04795"
     CDS             <1..110
                     /locus_tag="PK389_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019625351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HQY23052.1"
                     /translation="GPGGYRTKDFLKVGGGMTILYWAVMLISINLWFSK"
     gene            complement(151..1290)
                     /gene="hemW"
                     /locus_tag="PK389_04800"
     CDS             complement(151..1290)
                     /gene="hemW"
                     /locus_tag="PK389_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016393361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HQY23053.1"
                     /translation="MHNYIPLSLYIHIPWCVKKCPYCDFNSYTMEGNLPEKQYIETLL
                     RDLKQDLSLIQGRSIKSIFFGGGTPSLFSPKSIGEILDKASAFLDFEPNLEITLEANP
                     GTVEHHAFSDYQSAGITRISLGAQSFQNEKLAQLGRIHTIEETKCAIKSILAANFNSF
                     NIDIMYGLPNQTVADALFDLKMALSFSPPHLSWYNLTIEPNTIFHHKKPPLPLDDTLA
                     EMEQAGLEYLAKQELAQYEISAFSKPQHQCKHNLNYWKFGDYLGIGAGAHAKITDLKT
                     HTITRYWKTRYPKSYLETDHALLAGSQIILTEEIPLEFMLNALRLTAGTSVQEFETRS
                     GLPIASIATQLSDAEKKGLLIITENQKIKTSHLGKRFLNDLLTLF"
     gene            complement(1294..1869)
                     /locus_tag="PK389_04805"
     CDS             complement(1294..1869)
                     /locus_tag="PK389_04805"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HQY23054.1"
                     /translation="MVFLREAFIFLIKIIFDLYFLAVLLRFLFQYFQVDFYNPFSQFI
                     LKITDPVLAPLRNKLPHIRKIDTAALLLLFGLKIVAFLGITLLIKKMLPHVLGVVIWS
                     LGEIISQTINFFFFSVLLVAILSWINPRNYTPFTSLLIQITNPLMITARRWMPRTMGI
                     GFSSIIVLIILKLADVLVASPIIGLGIIFSY"
BASE COUNT          664 a          367 c          431 g          638 t
ORIGIN      
        1 caggacctgg ggggtatcgt acaaaagatt ttctaaaagt gggcgggggc atgacaattc
       61 tgtattgggc agtgatgcta attagcataa atctatggtt ttcaaaataa aaatacgttt
      121 tgagtcttga acttgagaag aagtctagag tcaaaacagc gtcaataaat cattcaaaaa
      181 tcgtttaccc aaatgtgagg ttttaatttt ttgattttca gttattatta ataaaccttt
      241 tttttcggcg tcgctaagct gagtcgcaat gcttgcgatg ggaagccctg accgtgtttc
      301 gaattcctga acagaagttc ctgctgttag tcttagtgca tttaacataa attcgagcgg
      361 tatctcttcc gtgagaataa tctggctccc agccagcaac gcatgatcag tttccaaata
      421 gcttttagga taacgcgttt tccaatagcg cgtaatcgta tgggttttga ggtcggttat
      481 ttttgcatgc gcccctgcgc caatgcctaa ataatcgcca aacttccaat aattcagatt
      541 atgtttgcac tgatgctgag gtttcgaaaa agcggatatt tcatattgcg cgagttcttg
      601 ttttgctaaa tattcaagac ccgcttgttc catttctgca agggtatcat ccaatggcaa
      661 tggtggtttt ttatggtgga atattgtatt gggttcaatg gtcaaattat accaagaaag
      721 atgcggtggc gaaaaagaca aggccatttt caaatcaaac agtgcatccg ccactgtttg
      781 gttgggtagt ccatacataa tatcgatgtt aaaactgtta aaattggctg ccaaaataga
      841 cttgatggcg cattttgttt cttctattgt atggatacgc cctaattgtg ctaacttttc
      901 gttttgaaag ctctgtgcac ctaaagagat acgtgtaatc ccggcacttt gataatcact
      961 gaatgcatga tgttctacag tgccgggatt cgcttcgagc gtgatttcaa ggttgggttc
     1021 aaagtctaaa aaagcactgg ctttgtctag aatttcaccg atgcttttag gtgaaaagag
     1081 acttggggtg ccgccgccga aaaaaatgct tttaatggaa cgtccttgaa tgagcgaaag
     1141 atcttgttta agatcacgca acaaggtttc aatatactgt ttttcaggaa ggttgccttc
     1201 cattgtataa gaattaaaat cacaataagg acatttctta acacaccaag ggatatggat
     1261 atacaatgat agcggtatat aattatgcat aaattaataa gaaaatatta tcccgagccc
     1321 aataataggg cttgcaacta aaacatcggc taattttaaa ataattaaga caataataga
     1381 cgaaaaaccg attcccatcg tacggggcat ccatcttctt gcggttatca tcagggggtt
     1441 ggtgatttgg atcaacagtg aagtaaaggg cgtgtagttt ctaggattta tccagcttaa
     1501 tatcgcaacc aagagcacag aaaaaaagaa aaaattaatt gtttggctaa tgatttctcc
     1561 caaagaccaa ataacaacac ccagaacatg tggtagcatt tttttaatga gtaaagtaat
     1621 ccctagaaac gctacgattt ttaatccaaa tagcaataaa agcgctgcag tatcaatttt
     1681 tcggatatga ggcagcttgt tacgtaaggg cgctaaaaca ggatctgtta tttttaaaat
     1741 aaattgcgaa aaaggattgt agaagtcaac ctgaaaatat tgaaatagaa agcgaagcag
     1801 cacggctaaa aaataaagat caaagatgat tttaatcaag aaaataaaag cttctcgcaa
     1861 aaaaaccata tattactcct tgtcgaacat ttggctaatc tcaacagaac ggtttttcgc
     1921 tgttgttaat gcatcgttaa ataaggtacg aataccaccg gattctaaat tctgtacatt
     1981 atatcattag gagagactac tttcattaag ctttgagcca tgtttccaca ccctacaaag
     2041 gcgataacag gttttttttg catggttttt ttataagggt ggatgactag cggatcgtgt
//
LOCUS       DAORVT010000214         2089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_56574_length_2089_cov_3.450344, whole genome shotgun sequence.
ACCESSION   DAORVT010000214 DAORVT010000000
VERSION     DAORVT010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56574_length_2089_cov_3.450344"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="PK389_04810"
     CDS             <1..685
                     /locus_tag="PK389_04810"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQY23055.1"
                     /translation="QIAEQFVGTSPNVMVGIGTTATQALISANQRYAIPIVFSSISDP
                     VGSKIVSDLKHPEGVVTGVSNFIDPALQFDLFKKILPKLTKLGVIYNPGEANSVALVE
                     QMKQAATQKDLELVITTANTTANVSQATHALISKVQAIFINNDNTALSAFDSIVKISA
                     KNKIPVFCSGVGMIKHGALATIGANQYMIGQQTGKMILEILGGKSPHDIPVAFPEKTE
                     IQLKNGDMP"
     gene            682..1563
                     /locus_tag="PK389_04815"
     CDS             682..1563
                     /locus_tag="PK389_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY23056.1"
                     /translation="MNMIQFLGALEIGLLYGLVSLGVYLTFRAINFPDLTVDGTFPLG
                     AAVAATLLVNGTHPILATAAAILAGSLAGFITGYLHVRWNILGLLAGILTMTALYSVN
                     LRIMGRPNIALLSELTLFSYFKGSHVVLWSMFGIIILVIYFLYRFLASQFGLALRASG
                     INPRVSPSYGINIGLMTLIGLAASNGLVALAGALFAQAHGFADISLGTGTLITGLASV
                     IVGESLLPKHRLLFMLIACILGSILYRLVIALALNSQFAGLQAQDLNLITAVLVVIAM
                     MVPKIRQNFTLKSRRTL"
     gene            1560..>2089
                     /locus_tag="PK389_04820"
     CDS             1560..>2089
                     /locus_tag="PK389_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQY23057.1"
                     /translation="MITLNNISVTFGKGTPLETTVFKDFNLKINPGEFVTVIGGNGAG
                     KSTLMNLISGDALSDSGSIILDNIAVTKWPTHKRAQLISRVFQDPLLGSYADLTIEEN
                     LSLAFSRGKSRTLTMALRENLREQFRERLSHMALNLENRLTDKMGLLSGGQRQAVSLV
                     MATLRPSKILLLDEHTA"
BASE COUNT          594 a          436 c          428 g          631 t
ORIGIN      
        1 acaaatcgcc gaacaatttg tcggaacttc cccaaacgtg atggtaggca tcggcacaac
       61 ggctacacaa gcgcttatat ccgcaaatca gcgttatgcc attccgattg tctttagctc
      121 gatttctgat cccgtaggtt caaaaattgt gagcgatctt aaacatccag aaggtgttgt
      181 cacaggtgtt tcaaatttta tagatcccgc gcttcaattc gatttattta agaaaatttt
      241 accgaagcta actaaactcg gtgttattta taatcccggc gaagcgaatt ctgttgcctt
      301 ggttgaacaa atgaaacaag ccgcaacgca aaaagacctg gaattagtga ttacaactgc
      361 aaatacaacg gcaaacgtta gccaggctac acatgcgctt atctctaaag tacaggctat
      421 ttttattaat aatgataata ccgcgctttc agctttcgat tctattgtaa aaatcagcgc
      481 taaaaacaag atccctgttt tttgtagtgg ggtcggtatg attaaacacg gtgcattagc
      541 aacgattggt gcaaaccaat atatgatagg acaacaaaca gggaaaatga ttctggagat
      601 tttaggtggc aaaagtccac atgacattcc agtggctttc ccagaaaaaa cagaaattca
      661 actcaaaaat ggggatatgc catgaatatg atccaatttt tgggtgcact cgaaatcggg
      721 ctattatatg ggctcgtttc tttgggcgta tatcttactt ttcgcgccat taattttcca
      781 gatctcactg ttgatggtac ctttccgctg ggtgcagccg ttgccgcaac cctactcgta
      841 aatggtacgc acccgatact cgcaactgcc gctgctattt tggcaggtag tcttgcaggg
      901 tttataacag gttacttgca tgttcgctgg aacattctcg gtttactggc cggaatatta
      961 accatgactg cactctattc agtcaatcta cgcattatgg gacgacccaa tattgcgctg
     1021 ctttctgaac tcacactatt tagttatttt aaaggatctc atgttgtttt atggagtatg
     1081 tttggcatta ttatattagt gatttacttc ttatatcgat ttttagcaag ccaatttggc
     1141 ttagcgttaa gggcaagcgg tattaaccca cgcgtaagcc cgagctatgg tattaatatt
     1201 gggttaatga cattgatagg tcttgccgca agtaacggat tggtcgcttt agcaggcgct
     1261 ttatttgctc aagcacatgg gtttgcagac atttcactcg gtacaggcac actgattacc
     1321 ggacttgctt cggttattgt cggagaaagt ctcttaccca aacatcgctt attatttatg
     1381 ttaatcgcct gtattttggg atcaattctt tatcgacttg tgatcgcgct tgctttaaat
     1441 agccaatttg ctggtttaca agcacaagac ttaaacctta ttacagcagt actggtcgta
     1501 atagccatga tggtacctaa aatacgccaa aactttaccc ttaaatctcg gcgaacacta
     1561 tgattacact taacaacatt tctgttactt ttggaaaagg cacaccttta gaaacaactg
     1621 tttttaaaga ctttaattta aaaattaacc ccggtgaatt tgtaactgtt attggtggca
     1681 atggcgctgg aaaatctacg ttgatgaatt taatttcagg tgatgcttta tccgattctg
     1741 gcagtattat tttagataat attgcggtca cgaaatggcc tacacacaaa cgcgcacaac
     1801 tcattagccg cgtctttcaa gatcccctat taggtagcta tgcagatctc acgattgaag
     1861 aaaatttgag tctcgctttt tcgcgtggaa aaagccgcac gctaacgatg gcgctacgcg
     1921 aaaatctgcg agaacaattt agagaaagac tcagtcacat ggcgcttaac ttagaaaatc
     1981 gtctaacaga taaaatggga ttgctctcgg gtgggcaacg tcaagccgtt agcctcgtta
     2041 tggcaacctt gaggccttcc aaaatattgc tcttggatga acatacggc
//
LOCUS       DAORVT010000215         2085 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_56779_length_2085_cov_2.244335, whole genome shotgun sequence.
ACCESSION   DAORVT010000215 DAORVT010000000
VERSION     DAORVT010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56779_length_2085_cov_2.244335"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="PK389_04825"
     CDS             <1..664
                     /locus_tag="PK389_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HQY23058.1"
                     /translation="ENGLNWQWSIELYGELLQITGGKERIRYYMERYSPGLLNRNDLK
                     DWIAHLHKTKTGFYEMLMEKGSIPLRPGVVRLIHELRDKKIMLAIATTTTLENVTSLL
                     KSTLGEESIDWFDVIGAGDIVPMKKPAPDIYHWVLNQLKLDARQCIAIEDSENGLKSA
                     MAANLLTLITVNGYTHLQDFSGALAILSDLGEPEKPFTQISGETFKQQWIDYGILNGL
                     LK"
     gene            815..1168
                     /gene="smbP"
                     /locus_tag="PK389_04830"
     CDS             815..1168
                     /gene="smbP"
                     /locus_tag="PK389_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small metal-binding protein SmbP"
                     /protein_id="HQY23059.1"
                     /translation="MKCIKSLLVIAIITLLISFPLVAADINPHTAEAIKHAESAQTHG
                     KAGHTKVLLEHAEESLKHAEAAENELSGANKQHASESVKHLNEAIEHAKQNHADEATK
                     HVSEALIHLQQSTGS"
     gene            complement(1219..>2085)
                     /locus_tag="PK389_04835"
     CDS             complement(1219..>2085)
                     /locus_tag="PK389_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019038524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQY23060.1"
                     /translation="RVLDNLSTGLQKNIDLFSNHPAFEFIQGDIRDFAICQKACEGID
                     YVSHQAALGSVPRSIKDPLTTNEVNITGFINMITAAKDAHIKTFVYASSSSVYGSEPT
                     LPKIENRVGEPLSPYAVTKKTNELYASVFHELYGMQIIGFRYFNVFGPRQDPNGPYAA
                     VIPLFIKGILDKTPVYINGDGEQTRDFTFVDNAVQANIRGLFCENQQAFGKVINVAVG
                     EKFTVNYMYDAIKDMLQSQHEATHRAPRAGDIRNSLADTSLAHALIGYAPTHRFMDGL
                     KETVNFFVKIYR"
BASE COUNT          655 a          374 c          444 g          612 t
ORIGIN      
        1 agaaaatgga ttgaactggc aatggagtat cgagctttat ggtgaattat tacaaataac
       61 gggtggtaaa gagcgcattc gctattacat ggaaagatac tctccaggat tattgaacag
      121 aaatgatttg aaagattgga tcgcacattt acataaaacc aaaacaggtt tttatgaaat
      181 gctcatggaa aaaggtagca ttccactacg gccaggagta gtccgattga tccatgaatt
      241 gcgtgacaaa aaaataatgt tagccattgc cacaaccacc acgttggaaa atgttacttc
      301 actgctaaaa tccacccttg gagaagagtc cattgattgg tttgatgtga ttggtgccgg
      361 agatattgtg ccgatgaaga aacctgctcc tgatatttat cactgggtat tgaaccagct
      421 taaattggat gcgcggcaat gtattgcaat tgaagactct gagaatgggt taaagtcagc
      481 aatggcggca aatctgctaa cattgattac agtcaacggt tacacacatt tgcaagactt
      541 ttctggtgcg ctagcaattt tgtctgattt gggtgagcca gaaaaaccgt ttacacaaat
      601 cagtggagaa acttttaaac agcaatggat tgattacggg attcttaatg gcctgttgaa
      661 ataatggtta atattgaatg ggttatgtga aagtaatttg aactactgtt aatcgtatta
      721 gtctattttt atagaattac actgttcact gtgagttagg aaactgaatg gcgaattctt
      781 tcttttaata acggagtata aaaaggatag gcttatgaaa tgcattaaat cattactagt
      841 aatcgcgatc atcacacttc ttattagttt tccgctggtt gctgcggata tcaatccgca
      901 tactgcagaa gcaataaaac atgcagaatc tgcacaaact catgggaaag caggccatac
      961 taaggtattg ttggaacatg cagaagaaag cttgaaacat gcagaagctg cggaaaatga
     1021 actttcaggt gccaataaac aacatgcttc tgaatctgtt aagcatctga atgaggctat
     1081 tgaacacgcc aaacaaaatc atgcagatga agctaccaag catgtcagtg aagctttgat
     1141 tcacttacaa caatctactg gcagttaata aatccaagct agattcctcg agtaaaacga
     1201 ggtttctagc ttaagaagct atcgataaat ttttacaaag aaattcacgg tttctttcaa
     1261 tccatccata aaacgatgtg ttggggcata tccaatcaag gcatgggcca atgaggtatc
     1321 tgccaaggag ttgcggatat ctccagcacg tggggctcga tgggttgctt catgttgaga
     1381 ttgcaacata tccttgattg catcgtacat atagtttacg gtaaattttt caccgacggc
     1441 tacattgatt actttaccaa aagcttgctg attttcgcaa aataggccgc gaatattggc
     1501 ttgtacagca ttatccacaa acgtgaaatc acgagtttgt tcaccatcac cattgatata
     1561 aacaggtgtt ttgtcgagta tgcctttgat gaaaagcggt ataacggctg catagggacc
     1621 gttgggatct tgtcttggtc caaatacatt aaaatatcta aaaccaatga tttgcatgcc
     1681 atatagctca tgaaaaacag aagcatatag ttcatttgtt ttttttgtta ccgcataagg
     1741 tgagagtggc tctcctacgc gattttcaat tttgggtagc gtgggttcac tgccgtaaac
     1801 agaggacgat gaagcgtata caaatgtttt aatgtgtgca tcttttgcag cagttatcat
     1861 gttaatgaag ccagtaatat tgacttcatt ggtagtgagt gggtctttta tcgagcgagg
     1921 aaccgagccc agcgctgctt ggtgggaaac ataatctatt ccttcacaag ctttctgaca
     1981 aatagcaaaa tctcttatat caccctgaat gaattcaaaa gctggatgat tactaaagag
     2041 atctatattc ttttgtagac ctgtgctcag gttatccaat accct
//
LOCUS       DAORVT010000216         2069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_57506_length_2069_cov_5.726415, whole genome shotgun sequence.
ACCESSION   DAORVT010000216 DAORVT010000000
VERSION     DAORVT010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57506_length_2069_cov_5.726415"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1043
                     /locus_tag="PK389_04840"
     CDS             <1..1043
                     /locus_tag="PK389_04840"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQY23061.1"
                     /translation="DKKLLDEAFSAGVTEIFAKHELDKITSYAAQFFRKKSNKAMSGN
                     ILYIEDNAAIANPTATILRESGYTVDHFVNGEEGIEAFRKNSYDLVLTDILLKGKLNG
                     YGTVKAIRHLEDENKKDIPLLVFSVLDDAAHKIELLRLGANDYVTKPLVQEELLARVS
                     NLIINKKLLDKMRMQRCSTSESVISAMKDPVTGLYNYDFLMETALSRLREATRHNISY
                     SLINVSIDQLKQISETYGQSTGDCVLKEIASILSQSIRKEDVAARHEEEEFVLLLSHC
                     DISNAAIIAEKIRKSIENLKPANLSITASFGVAEISQDSMEDFTELSKAANQAVCQAK
                     ACGGNTVVAHDC"
     gene            1135..1236
                     /locus_tag="PK389_04845"
                     /pseudo
     CDS             1135..1236
                     /locus_tag="PK389_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558077.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            1540..>2069
                     /locus_tag="PK389_04850"
     CDS             1540..>2069
                     /locus_tag="PK389_04850"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HQY23062.1"
                     /translation="MVKNTAPIKVRSVWISDVHLGFRGCQADALLHFLHSVETDYLFL
                     VGDIVDFWSIKKNPYWPQEHTNVIRSILGKAKHGTKVIYIPGNHDEALRDCVSHVFGN
                     IEIHQDYVHTSAEGKKLLIMHGDEFDVIVKNSRWLAKLGNVAYDTLLDLNHYINSLRK
                     FFGFSYWSLAAYLKLKV"
BASE COUNT          678 a          381 c          418 g          592 t
ORIGIN      
        1 aggataagaa gttattggat gaagcatttt cagccggtgt gacggaaata tttgctaagc
       61 atgaattaga taaaattacc agttatgcag cgcagttttt taggaagaaa tctaataaag
      121 ccatgtctgg caatattctt tatatagaag ataatgcagc aattgcaaat ccgacagcta
      181 ccattcttag agaaagtgga tatacggtcg atcatttcgt aaacggtgaa gaaggtatcg
      241 aagctttcag aaaaaattcc tatgatttag tgctgacgga tatattgctt aaaggaaagc
      301 ttaatggcta tggaaccgtc aaggcaatac gtcatcttga agatgagaat aaaaaagata
      361 ttcctttatt ggtattttca gttttggatg atgccgcgca caaaatagag ttattgcgct
      421 taggtgccaa tgattacgtc accaaaccgt tggttcagga agaattgctt gcaagagtca
      481 gcaatttgat tattaacaag aaattattag acaagatgcg tatgcagaga tgtagcacaa
      541 gtgaatcagt catttctgcc atgaaagatc cggtaacggg tttgtacaat tatgattttc
      601 tgatggaaac cgcactatcc aggctgcgtg aagcaacacg acacaacata tcctacagtc
      661 taattaacgt tagtattgat caactcaaac aaattagtga aacatatggc caatcaacgg
      721 gcgattgcgt attgaaagaa atcgcttcta tactttctca atctattcga aaagaggatg
      781 tcgctgctcg ccatgaagaa gaggaatttg tgttgctatt gtcacactgc gatatttcaa
      841 atgcagcgat cattgcagaa aagattcgta aatcgattga aaatctaaag cctgctaatt
      901 tgagtataac cgcaagcttt ggtgtcgctg aaataagcca agattcaatg gaagatttta
      961 ccgagctatc caaagcagcc aatcaagccg tgtgtcaagc aaaagcatgt ggcggcaata
     1021 cagtggttgc acacgattgc tgagatttcc tgtagtaatt cagacagcct gttgaaaaac
     1081 ccctgatact acgttgaatt gataataaaa tagaggcttc accttcgacc actggtaagc
     1141 tttgagctgc atttaaaaga atccgaatgg cgttggaaaa aacaacctga tgaacttgat
     1201 agtgaactat caaaactcat cagctattat caatagctgc tagtttagtg ctttataaaa
     1261 agtataggtt agtaaactaa ttatcacaag aaggtacaaa tatgccacca ggtaacaaaa
     1321 gtgtttccaa gataaaatag acctctacac aaccgtaaga attttgctgt agtgacagaa
     1381 aaaatttcca aatgacagtt gttgcacgtc attttgatag aaagcgccct ctcctctttt
     1441 taataaacat ttcatgaata tttaacaaat acttcaacgt agcttcataa accctcttta
     1501 agctgtgatg ccatgaataa cccaattaaa aaggcattta tggtgaaaaa tacagctcct
     1561 atcaaagtca gaagtgtttg gatctcagat gttcatctcg gatttcgcgg ttgccaagcc
     1621 gatgcactct tgcattttct gcattctgtc gaaacggatt atctttttct ggtcggcgac
     1681 attgtcgatt tttggagcat caaaaagaat ccttactggc cgcaagaaca cactaatgta
     1741 attcggtcta ttttaggcaa agctaagcat ggcactaaag tcatttatat tcccggcaat
     1801 catgatgaag cgttgcggga ttgtgtcagt catgtattcg gcaacatcga gattcatcaa
     1861 gattatgtgc acacatcagc cgaaggaaaa aaattgctga taatgcatgg cgatgaattt
     1921 gatgtcatcg tcaaaaatag ccgctggctt gctaaattgg gaaatgtcgc ctacgacact
     1981 ttattggacc tcaatcacta tatcaatagc ttgcgcaaat tcttcggttt ctcctactgg
     2041 tcactggctg cttatctaaa attgaaggt
//
LOCUS       DAORVT010000217         2062 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_57869_length_2062_cov_2.964126, whole genome shotgun sequence.
ACCESSION   DAORVT010000217 DAORVT010000000
VERSION     DAORVT010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57869_length_2062_cov_2.964126"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="PK389_04855"
     CDS             <1..823
                     /locus_tag="PK389_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQY23063.1"
                     /translation="PLELLESELFGHEKGAFTGAVVTRQGKFELAAGGTLFLDEIGDM
                     PMSMQAKLLRVLQERTFERVGSSKSIQANVRILAATHKNLEACIAEGTFREDLYYRLN
                     VFPIEIPPLRERIKDISLLMGHFIEKHKKETGNFITLSPETITFLEQYNWPGNIRELC
                     NLVERLLILYPGKCVSVDDLPAQLFRQPLKHEKNLTTLMMSELVREDRQFDLKGHLEK
                     VERSFILDALENCGGVVARAAKRLGLRRTTLVEKIRKYGIGRNNEDKEQPKELFS"
     gene            complement(1177..>2062)
                     /gene="prmA"
                     /locus_tag="PK389_04860"
     CDS             complement(1177..>2062)
                     /gene="prmA"
                     /locus_tag="PK389_04860"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HQY23064.1"
                     /translation="GTNQDFAEIYSEYFTLAGAVAVTLHDTKDDPLFEPAPNTTPLWQ
                     ETTLVGLFDADVDITSVKKFLNKHLDNQSFQTLKIDPLEERDWIRAGTAQWEPMRFGK
                     KLMICPSWSENPDPTAITLMLDPGLAFGTGTHATTRLCLEWLDQHPPMGATVVDYGCG
                     SGILGIAALKLGAQFVYAVDNDPQALLSTQNNAKNNGFSEKNILTLLPTALNNALQKS
                     TTPPKKANLILANILANPLITLAPIFAELVEVNGMIVLSGFLENQIESVFEAYKPWFK
                     MLETDTIDGWVRLSAKAV"
BASE COUNT          626 a          361 c          462 g          613 t
ORIGIN      
        1 tcctttagag ttactcgaaa gtgagctttt tggtcacgaa aaaggggctt ttacaggtgc
       61 agttgttacg agacaaggca agtttgaatt agcagcaggc gggactttat ttttagacga
      121 aattggtgat atgccaatgt ctatgcaagc aaaattatta cgcgttttac aagagcgaac
      181 ttttgaacgg gtgggaagca gcaaatcgat ccaagccaat gtgcgtattc ttgcggcgac
      241 gcataaaaat cttgaagcat gtattgcaga gggtaccttt agagaagatt tatattatcg
      301 actgaatgtt ttccctatcg aaattccacc tcttcgagaa cgaatcaaag atatcagttt
      361 gctgatgggg cattttattg aaaaacataa aaaagaaacg ggcaatttta taacgctttc
      421 accagaaact ataacctttt tggaacaata caattggcca gggaatatac gtgagctgtg
      481 taatttagtt gaacgtttgc ttattttata tcctggaaag tgtgtgtctg tcgatgattt
      541 gcctgcacag ctttttcgtc aacctcttaa acacgaaaag aatctaacaa cgcttatgat
      601 gtcagaattg gttagggaag atagacaatt tgatttaaaa ggacatcttg aaaaagtaga
      661 acgttctttt attttggatg cattagagaa ttgtggaggt gttgttgcaa gagcggccaa
      721 aagactgggg ttgcgacgaa cgaccctggt tgaaaaaata agaaaatatg gcattggacg
      781 aaataacgaa gataaagaac aaccgaaaga attattcagt taatttgagt ctgccccaca
      841 gtaattggca ttcatgaatt ttacatttta aaaatatttc taaataaata catccgatat
      901 ttatacgaca aattaaaaaa ccagatcaaa ttaggataat tgacaatctt gactcattgt
      961 tcgcatcttg aagtgtaatg ggtatagcta tacttaaatt atgcacaaag taaacgcctg
     1021 tataatgaac actcattggc atcattgtta ttccagcaag cagttgattt gggcgatgtt
     1081 gctgcgaaat ttaatttagg acttttgtac aactagaaat tgttaaatta ctgcgtcaga
     1141 ttacgggaat tggtacgcca tcatgcaaaa tagttgctaa acagctttag cgcttaaccg
     1201 cacccaacca tcgatggtgt cggtttctag cattttaaac cagggcttat aagcttcaaa
     1261 aaccgattca atttgatttt cgagaaaacc ggataaaaca atcatgccgt tgacctcgac
     1321 aagttctgcg aaaatgggcg caagcgttat gagtgggttt gcaagaatat tagcaaggat
     1381 cagattcgct tttttgggtg gcgttgtgga tttttgtagc gcattattga gtgccgtggg
     1441 taatagtgta agaatatttt tttctgagaa gccattattt ttggcattat tttgcgtact
     1501 taaaagcgct tgcggatcat tatcaacagc ataaacaaat tgagcgccca gctttaaagc
     1561 ggcgatcccc agaatacccg aaccacagcc ataatccacg acggtggcac ccattggtgg
     1621 atgttgatcg agccattcta aacagaggcg tgtggtggca tgtgtgccgg taccaaaggc
     1681 taatccggga tctagcatta aagtaattgc ggtgggatca ggattttctg accagcttgg
     1741 gcaaatcatg agtttttttc caaaccgcat gggttcccat tgcgcggttc ctgcacggat
     1801 ccaatctctt tcttccagcg ggtcgatttt aagtgtttga aagctctgat tatctaaatg
     1861 cttgtttaag aattttttaa cagaggtgat gtcgacgtcg gcatcaaata gtccaacaag
     1921 ggttgtttct tgccagagtg gcgtggtgtt gggtgcaggc tcaaataagg gatcatcttt
     1981 tgtatcatgc aatgtaacag caactgcacc tgcgagggta aaatactctg agtagatttc
     2041 ggcaaaatct tgattggtac ca
//
LOCUS       DAORVT010000218         2049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_58521_length_2049_cov_3.107322, whole genome shotgun sequence.
ACCESSION   DAORVT010000218 DAORVT010000000
VERSION     DAORVT010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58521_length_2049_cov_3.107322"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="PK389_04865"
     CDS             <1..775
                     /locus_tag="PK389_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23065.1"
                     /translation="AMRLVRFKSRIKGGFSKTNLYSPVNQAAPINALGNQKVMGTLAL
                     GAQYKYCNFKAQVWGYQFYDIAKLYYGDLHYTMDTDTLVRPIAGVQVGRERNDSNSVF
                     NAARVARKNSDVYGALVGAEIVDGSVTIGYNNIPKHTGSFGNGNIVSPYTAGYAADPL
                     YTTSMIAGLIDKSAGSATKGTIQYNFFEKQLHLLVSYARYNTSPFTPNTSEANFDLTY
                     KPNEQCKNLSLRYRMGLLRHNPAFGRLIYNRLMVQYDFS"
     gene            793..2043
                     /locus_tag="PK389_04870"
     CDS             793..2043
                     /locus_tag="PK389_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQY23066.1"
                     /translation="MEARALGIQNFGEMLMNTQNYFCFFFITFFMFSEPCFSEHVAIQ
                     PKKSVIKIGMTTALSGPAQALGEEVKIGVETFFARINAEGGVAGHKLTLITLDDQYNP
                     KLAAQNMHLLADEEKILAVIGNVGTPTAEKTVPIANERKILLFGAFTGGNVLRKTPPD
                     RYVINLRAGYAEEAENMVKGLLSIGVKPDDFAFFLQHDNGADAGYFGALNALIASGYN
                     NTQSLPVGRYTRNTLNVEGGMASLIAAGKSPKAILMVGAYAPIAKFIMLAKKAFPSTL
                     FLNVSFVGSSSIIRLLGADSDNIIFTQVVPPPNSDLTAAQDYREDLNKYSPGSQPSYG
                     SFEGYLDAKLFVVGLTEAIAKNALTREGLVDAFESLQNIDIGIGLKIGFDKNNHQALH
                     TIWPTIIKNGQIEPLDWSKLKMPN"
BASE COUNT          626 a          373 c          439 g          611 t
ORIGIN      
        1 ggcaatgcga ctggttcgtt ttaaaagccg aatcaaaggc ggcttttcga aaaccaatct
       61 ttatagtcca gtcaaccagg ctgcacctat aaacgcgcta ggtaatcaaa aggtcatggg
      121 tacgcttgct ttaggcgcac aatacaaata ttgcaatttt aaagcgcaag tgtggggcta
      181 tcaattttat gatattgcaa agctttatta cggcgacctt cattatacaa tggatacaga
      241 tactttagta cgaccgatag caggggttca ggtaggtcgc gaacgtaatg attcaaattc
      301 tgtttttaat gcagcacgtg ttgcgagaaa gaattctgat gtctatggcg cattagtggg
      361 cgctgagatt gtggatggca gtgtgactat tggttataac aatattccca agcatacggg
      421 ttcatttgga aatggaaata tagtgtcccc ttatacagcc ggttatgctg ccgatccact
      481 ttatacaacc tctatgatag cgggacttat agataaatca gcaggctcag caacaaaagg
      541 aaccatacaa tacaattttt ttgaaaaaca gcttcattta ttagtgagtt atgctagata
      601 taatacaagt ccatttacac ctaacacatc cgaagcaaat tttgacctga cttataaacc
      661 gaatgaacaa tgtaaaaatc tttcacttcg ctaccgtatg ggtcttttaa gacataatcc
      721 ggctttcggt cggcttattt ataatcgttt aatggtgcaa tacgatttta gttaatcaac
      781 agggattaca atgtggaagc acgtgcactt ggaattcaaa acttcggcga aatgttaatg
      841 aatacccaga attacttttg tttttttttt attacttttt ttatgttttc cgagccctgt
      901 ttttcagaac atgttgctat acaaccgaaa aaatctgtca ttaaaattgg aatgactacc
      961 gcattaagcg gtccggcgca agcactcggg gaagaagtca agattggtgt agaaaccttc
     1021 tttgctagaa taaatgcgga gggtggagta gctgggcaca aattgacatt aattacattg
     1081 gatgatcaat ataatccaaa acttgctgct caaaatatgc atctgttggc ggatgaggaa
     1141 aaaattcttg cggttattgg taatgtaggt acgcctacag cagagaagac cgtgcccatt
     1201 gccaatgagc gtaagatact gctttttggg gcatttactg gcggtaatgt actacgtaag
     1261 acaccacccg atcgttatgt cataaatctt cgtgccggtt atgcagaaga agctgaaaat
     1321 atggtaaaag gattattatc gataggtgtt aaaccggatg actttgcttt ttttctacaa
     1381 catgataatg gtgctgatgc gggttatttt ggtgcactaa acgcgttaat agcgtcggga
     1441 tataataata ctcagagttt acctgttggt cgctacactc gaaatacttt aaacgtagaa
     1501 ggtggaatgg ctagtcttat tgcagctgga aaatcaccaa aagccattct catggttggt
     1561 gcttatgcgc caattgcaaa attcataatg ttagcaaaaa aggcttttcc tagtacactt
     1621 ttcttgaatg tatcttttgt gggaagttca tctattataa gattactggg agcggatagt
     1681 gacaacatca tttttacgca agtagtaccg ccacccaatt cggatttaac tgctgcacag
     1741 gattatcgag aggatctcaa taaatatagc cctggatcgc agccaagcta tggttctttc
     1801 gaaggctatc ttgacgctaa actttttgta gtgggtctta cagaagcaat agcaaaaaac
     1861 gcgttaacgc gcgaaggtct tgttgatgct tttgaaagtt tgcaaaatat tgatataggt
     1921 attggcttga aaattggttt cgataaaaat aaccatcaag cgctacacac aatatggcct
     1981 accattataa agaatggtca aattgagccc ttggattggt ccaaactaaa gatgccaaac
     2041 taggagcgc
//
LOCUS       DAORVT010000219         2045 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_58734_length_2045_cov_3.256784, whole genome shotgun sequence.
ACCESSION   DAORVT010000219 DAORVT010000000
VERSION     DAORVT010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58734_length_2045_cov_3.256784"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PK389_04875"
     CDS             complement(<1..182)
                     /locus_tag="PK389_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HQY23067.1"
                     /translation="MKDGNKMSFVLDASIALAWCFSDESTAFTHKLLVQLENDIALVP
                     TIWSLEVGNILIMAERR"
     gene            complement(166..405)
                     /locus_tag="PK389_04880"
     CDS             complement(166..405)
                     /locus_tag="PK389_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HQY23068.1"
                     /translation="MQSIGAFEAKTHFSALLEQVEKGQEMLITKHGRVVAKLSPVGFN
                     KSHLKEAVEDIEKFQNDHKLTLGLDWKILRDEGRK"
     gene            650..1033
                     /locus_tag="PK389_04885"
     CDS             650..1033
                     /locus_tag="PK389_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23069.1"
                     /translation="MNHSENKHAHTPLHEQGSAEHGSKCGSGKQHKSYTKLCPLSLGI
                     ASGFVNGLSVLVLAWLGSSAHMHAEVVRTMGVSGVWGAVLFGFIAGFVGALIFAWIYN
                     VCLCCWKCRSKCSKCDSSCCKCSSH"
     gene            1243..>2045
                     /locus_tag="PK389_04890"
     CDS             1243..>2045
                     /locus_tag="PK389_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HQY23070.1"
                     /translation="MSIFRTKMLHQTDTEGELKRCLTAFDLTLLGIGAIIGAGIFVIT
                     GIVAATQAGPGIVLSYGLAGIACSFAALTYAELAASIRGSGSAYNYAYAGLGEIVAWI
                     IGWDLILEYGVGTSVVAIGWSGYLNDALLSIGISIPVQLLKNYAEGGFINLPAVLIIL
                     TLCGVLMIGVKQSARFNMLIVFIKLAAIALFIAVASTEVQPHNWTPFMPFGWKGVMEG
                     AALVFFAFIGFDAVSTAGEEAKNPQKDLSLGIIGSLGICTALYIAVSGL"
BASE COUNT          553 a          386 c          458 g          648 t
ORIGIN      
        1 cggcgctcag ccataataag tatatttccc acttctaatg accaaattgt gggaactaaa
       61 gcaatatcat tttctaattg tacgagcaat ttatgcgtaa aagcggtgct ttcatcagaa
      121 aaacaccatg caagcgcaat cgatgcgtct agtacaaaac tcattttatt tccgtccttc
      181 atctctcagt attttccaat ctaacccaag cgtgagctta tgatcgtttt gaaatttttc
      241 aatatcttca acagcttctt taagatgcga tttattaaac ccaacaggtg acaattttgc
      301 cacaactcta ccatgcttag tgataagcat ttcttgtcct ttctcaactt gttctagcaa
      361 agctgaaaaa tgcgtctttg cttcgaaggc accgatagat tgcatataca tatcctctta
      421 tactggtctt aaactagtct aaagtttagc ttaaaaggac ggaaaggtca aactttttga
      481 agtgtggatt atcttaaagc tacaatcaag acaatcatgg aggaaggcgg ctggaaacgt
      541 agtgaagctt aactttctct agttttaaat aggtcaagca gccaaaaaaa tttactttct
      601 tctatactta agaatgatta atgcacattt tggtatggag gaaggaaata tgaatcattc
      661 agaaaataag catgcacaca cacctctgca tgaacagggt agcgctgagc atggaagtaa
      721 atgcggcagt ggtaaacagc ataaaagcta caccaagcta tgccctctgt ccttaggtat
      781 tgcatctggt tttgttaatg gattgagtgt tttagtcctc gcttggttgg gctctagcgc
      841 gcatatgcat gctgaagtcg tgcgtacgat gggtgtttcc ggcgtttggg gtgcagtgct
      901 ctttggattt atcgccggat ttgtgggtgc attgatattt gcatggattt ataacgtatg
      961 cctgtgttgc tggaagtgtc gatctaaatg cagcaaatgt gactcatctt gttgtaaatg
     1021 ttcatcgcat taaatagtac tgtttaagta acgctaacaa caatgggttg ctcggtgggc
     1081 tgttttcagt ttgccgagca attctattgc ctattgaaaa aaaatgttat tatgttctac
     1141 tgaataccgc cttaattcta actaggcctg agatttttag aataaagtta aataactttt
     1201 aaatatgagt agtggcttaa cgagagagga attttacagg acatgagtat atttagaaca
     1261 aaaatgctgc atcaaaccga tacggaaggc gagctaaaac gctgcttaac cgcctttgat
     1321 ttaacgttat tgggcatcgg cgcgatcatt ggcgcaggca ttttcgtgat aaccggtata
     1381 gtagctgcga cgcaagcggg tcctggtatt gttctctctt acgggctcgc gggtatcgcc
     1441 tgctcctttg cggcattgac atacgctgaa ctggcagcca gtattcgtgg atcgggcagt
     1501 gcttacaatt acgcatacgc gggactcggc gaaattgttg cttggattat cggttgggat
     1561 ttaattttag aatacggtgt cggaacttct gttgttgcga ttgggtggtc tggatatctc
     1621 aatgatgcgc tcttgtccat aggcatttca atccctgtac aattacttaa aaactatgca
     1681 gaaggaggat ttattaatct tcctgctgtt ttgattatct taacgctgtg cggggtgtta
     1741 atgatcggcg taaaacaaag cgcacgcttt aatatgctga ttgtctttat taaattggcc
     1801 gctattgcgt tatttattgc agtagcctca acagaagttc agccccataa ttggacaccg
     1861 tttatgcctt ttggatggaa aggtgttatg gaaggagcgg ctttagtctt tttcgctttt
     1921 attggatttg acgccgtttc aaccgcaggc gaagaagcca aaaacccaca aaaagactta
     1981 tcgttaggga ttattggttc gctgggtatt tgtacggcgc tttacattgc cgtgtctgga
     2041 ttgtt
//
LOCUS       DAORVT010000220         2044 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_58780_length_2044_cov_3.719457, whole genome shotgun sequence.
ACCESSION   DAORVT010000220 DAORVT010000000
VERSION     DAORVT010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58780_length_2044_cov_3.719457"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1559)
                     /locus_tag="PK389_04895"
     CDS             complement(<1..1559)
                     /locus_tag="PK389_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23071.1"
                     /translation="MSLPGIQTARRVQSTLEAKEVLDATRSSGLHFFVTTLLSEKIKS
                     ALDAFTVTRSDNNAHIIELPTLDPQAHLGTSDCSILKQSHFIERHLRVDEFFAPGSSA
                     NGQHPYPYCHYTETYETPDSKRIVLHLYINKECKIEHCQIKIIGTTEGADGNSIEAVS
                     YFSDDVVNILKPLAIKNANTALNLIKKLNVQKLDTFLSLHKETQDLDSQLSRLSANLY
                     QKKARAEYLRSGKKFIENIKALNNLTDSRYADTRDMHIKRMCARINKGDFTPKTFESS
                     EIIETTISVDHQTEPSAGPDSQEALLTISEQKRQSAKNKFKLAISAQRAELLGMIQKD
                     KEQLDGFPKKEEMIDAKQLIERLKIVDNLRCNLLALYCLPDNPQKEPSVISALEKKLD
                     KIPTLLQSFETCAKAGKLSEVSELHPHIESHLSVSFIHQLVEDVLHNQKNNLVERIGV
                     CDYLYTNSALYRIAIEALNYTFTINNSGLAVSSLVTACDLQNKLAFDMLLRQGANPNE
                     AGAIYGECIVP"
     gene            complement(1808..>2044)
                     /locus_tag="PK389_04900"
     CDS             complement(1808..>2044)
                     /locus_tag="PK389_04900"
                     /inference="COORDINATES: protein motif:HMM:NF037782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase"
                     /protein_id="HQY23072.1"
                     /translation="YCHQFTHLRSLSHMGPIALYDGKNSAEDLLLAAQIVARFGGGQF
                     ADTVRIEINTHVDEARIVEVAPIKAENIAEHWYI"
BASE COUNT          547 a          376 c          414 g          707 t
ORIGIN      
        1 ataggcacaa tgcattcacc gtaaatcgca ccagcttcat ttggattggc gccctgacgt
       61 aatagcatgt cgaacgccaa tttattttgc agatcgcaag cggtgactaa cgaagaaaca
      121 gccaagccac tgttgtttat tgtaaaagtg taattgagag cttctattgc aatacgatat
      181 aaagctgaat ttgtatacaa ataatcacag acaccaatcc gctcaaccaa attattcttt
      241 tggttatgta aaacatcctc tactaattga tgtataaaag atacgctcaa gtggctttca
      301 atatgtgggt ggagttcaga aacttcagaa agttttcccg cttttgcaca tgtttcaaaa
      361 ctttgtaata aagttggaat tttgtccagc tttttctcaa gtgcgctaat aacactgggt
      421 tctttttgag ggttatcggg cagacaataa agagccaaca aattgcagcg gaggttatct
      481 acaattttaa gacgttcaat taattgtttt gcatcaatca tttcttcttt ttttggaaaa
      541 ccatcgagtt gttctttatc tttttgaatc atccccagta actcggcgcg ttgtgcactt
      601 attgccaatt taaatttatt tttagcgctc tgtcgttttt gttctgatat tgtcaaaagg
      661 gcttcttgtg aatcaggtcc tgcgcttggt tctgtttggt gatctactga gatggttgtt
      721 tctatgattt cggaagattc aaaagttttg ggcgtaaagt ctcccttgtt aatgcgcgcg
      781 cacattcgtt ttatatgcat gtcccgagta tctgcataac ggctatcggt tagattgttt
      841 aacgctttaa tgttttcgat aaatttttta cctgatctta aatactcagc gcgtgctttc
      901 ttttgatata aattcgcgga caatcggctc agttgggaat caaggtcttg cgtttcttta
      961 tgcagagaca aaaacgtatc tagtttttgt acgtttaatt ttttgattaa atttaaggca
     1021 gtgttggcgt tttttatcgc taaaggtttt aatatgttca cgacatcatc tgaaaaataa
     1081 ctaacagctt caatcgagtt gccatcagct ccttcagttg ttccgataat ttttatttgg
     1141 caatgttcta ttttgcattc tttattaata tacaagtgta gcacaatacg cttgctgtca
     1201 ggcgtctcat aagtttcggt gtaatgacaa taagggtatg ggtgttgccc attggcagaa
     1261 gaaccagggg caaaaaattc atctacacgc aagtggcgtt ctataaaatg ggattgcttt
     1321 aatatagaac aatcagacgt gcctaaatgt gcctgtggat ctaatgtagg caattctata
     1381 atgtgagcat tattgtctga tcgtgtaacg gtaaaggcat ctaaagcact ctttattttt
     1441 tctgaaagca gtgttgtaac gaagaaatgt aagccgctgc ttctcgttgc gtctagcact
     1501 tctttggcct ctagtgtcga ttgtactctc cgtgcggttt ggatgccggg caaactcatg
     1561 aatgctctcc tggatgtgta tttcttagca aaaaatttac ataatctccg gggattacgc
     1621 taacatattg aattgaatat ttatagctgt tttttgtaca gtgtgtcgtt cttgtgaaaa
     1681 attgagataa ataattttaa tgttttgaca gaatggctga gagatctcgt ggcacgagaa
     1741 atcaatctat tgctttttta ttctttttgc atccagtgtt acatcttacg tcagcggttg
     1801 ttgcatgtta aatataccag tgttcagcaa tattttcggc ttttatgggg gcaacttcta
     1861 caatgcgtgc ttcatcgaca tgtgtattaa tttcgatacg gacggtatct gcaaattgtc
     1921 caccaccaaa tcgagccaca atttgagcgg ccagcagcaa gtcttccgca ctatttttgc
     1981 catcgtatag tgcaatcggg cccatatggc tcaacgatct taagtgcgta aactggtggc
     2041 aata
//
LOCUS       DAORVT010000221         2043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_58829_length_2043_cov_3.865694, whole genome shotgun sequence.
ACCESSION   DAORVT010000221 DAORVT010000000
VERSION     DAORVT010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58829_length_2043_cov_3.865694"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PK389_04905"
     CDS             <1..280
                     /locus_tag="PK389_04905"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HQY23073.1"
                     /translation="SAFKAQVWIAAFAATILILGASYTLWMTKRVVFGPAEQPGVLSL
                     QDLDATEKGGFSLLALAILVLGLWPAPLLNIIHASADHLVSQILHSKI"
     gene            300..1748
                     /gene="nuoN"
                     /locus_tag="PK389_04910"
     CDS             300..1748
                     /gene="nuoN"
                     /locus_tag="PK389_04910"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HQY23074.1"
                     /translation="MQLSELTNVLAEIWLLAMSILILLVDLFISRGSKTTVYFLTQVA
                     LIGVLFFTANLIGEAPKTLFNQMFVVDDIANALKFSIALVGFFVFLYSRQFVFGSPTY
                     KSEYFVLCLFSIFGMMVLISARSFLTLYLGIELFALPLYALIPILKKDGIAPEAAMKY
                     FVMGALASGLLLYGISLLYGVTGSFQLQEIASVLAEQMQHPHPALLFGLVFVLVGLAF
                     KFGAVPFHLWLPDVYQGAPLSVTLFIGTLPKLAAFGFAIRLLIDTFMSLALYWQPILV
                     VMSVLSLTIGNVVAIAQTNLKRMLAYSTIAHVGFLFLGLLAGPASGFAAALDYVMIYV
                     LMALGAFGIMTALSAQGFEAENIADFRGLGVRNPWLAFLMMMVLFSLAGIPPFAGFYA
                     KLLVLNALVNAGYVWLAVFAVLLTVVGAYYYLRVIRVMFFDAPEGNNFLQPVSVLRLV
                     ALSFNGLAILVLGIFPAPLFNLCLSVLPKPLF"
BASE COUNT          446 a          352 c          513 g          732 t
ORIGIN      
        1 aagtgcattt aaggcgcaag tttggattgc tgcttttgcc gcaactatcc tcattttggg
       61 ggcttcttat actttatgga tgactaaacg tgtggtattt ggacctgcag agcaacctgg
      121 agtgctgtct ttacaagatt tggatgcaac agaaaagggt ggctttagct tgttggccct
      181 tgccatttta gtgttggggc tttggcctgc gccattgctg aatattattc atgcttctgc
      241 ggatcatctt gtgtcgcaga tcttacattc aaaaatttaa aaaaaggaag ggtggggtga
      301 tgcagctttc agaattgacg aatgtactgg cagaaatatg gctgcttgct atgagtattt
      361 taattttatt agtcgatttg tttatttctc gagggtcaaa aacaactgtc tattttttga
      421 cacaagttgc gttaattggt gttttatttt ttacagcaaa tttgataggt gaggcgccaa
      481 aaaccttatt taaccagatg ttcgttgtcg atgatattgc gaatgcattg aaatttagca
      541 ttgcattggt tggttttttt gtatttttat attctagaca atttgtattt ggaagtccta
      601 cctacaaaag tgaatatttt gtgctgtgtc ttttttcgat ttttgggatg atggttttga
      661 tttctgcgcg cagtttttta acgctctatc tcggtattga acttttcgct ttgcctttgt
      721 atgcgttgat ccccattctt aaaaaggacg gcattgcgcc agaagcggcc atgaaatatt
      781 tcgttatggg cgctttggca tcgggtttgt tattgtatgg catctctttg ctttatggcg
      841 taaccggcag ctttcaattg caggagatag cgagtgtgtt ggcagagcaa atgcagcatc
      901 ctcatcctgc tttgctattt ggtttagtgt ttgtattagt gggattggct tttaagtttg
      961 gagctgtgcc ttttcatctt tggttgcctg atgtctatca gggtgcgccg ttgtcggtta
     1021 cgctgtttat tggcacatta ccaaaattgg cggcttttgg atttgcgatt cgattattaa
     1081 tagatacgtt tatgtcattg gcgctttatt ggcagccgat actcgtggtt atgtcggttt
     1141 tgtcgttaac aatagggaat gtggttgcga ttgctcaaac gaatttaaaa cgcatgttgg
     1201 cgtattcgac gattgcacat gttggatttt tatttttagg acttttggca gggccagcat
     1261 cgggatttgc cgcagcctta gattacgtga tgatttatgt gctaatggcc ttaggtgctt
     1321 ttggtattat gacggctttg agtgctcagg ggtttgaggc agaaaatatt gccgattttc
     1381 gtgggttagg agttagaaat ccttggctag catttttgat gatgatggta cttttttcac
     1441 ttgcggggat cccgcccttt gccggcttct atgcaaaatt attggtgtta aatgcgttgg
     1501 tgaatgcagg gtatgtgtgg ctcgcagtat ttgccgtgct gttaactgtt gttggtgctt
     1561 attattattt acgcgtgatc cgggtgatgt tttttgatgc gccagaaggc aataattttt
     1621 tgcaacctgt ttctgtgttg cgtcttgtgg ccttgtcttt taatggttta gccattttag
     1681 tcttagggat ctttcctgcg cccttgttca acttgtgttt gtctgtgtta cccaagccac
     1741 tattttagtt gataagctta ttcagatatc ttgcactaaa acgataaata agtgatttgc
     1801 tgcaggcttt tataatcaat agacaaatgt ggttaaacaa aggaacgaca ggatcaaagc
     1861 cgtggcgccg agcacgggtg cgcctgcaaa accgtcgtag ccaaataggc tatggcggcg
     1921 accacaagtc tcatggctga taattctgct tgcaatcgct gctctaagta ggtagtatat
     1981 ctactagttg acgcggggtg gagcagtctg gcagctcgtc gggctcataa cccgaaggtc
     2041 gta
//
LOCUS       DAORVT010000222         2033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_59431_length_2033_cov_2.141052, whole genome shotgun sequence.
ACCESSION   DAORVT010000222 DAORVT010000000
VERSION     DAORVT010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59431_length_2033_cov_2.141052"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1530
                     /gene="traN"
                     /locus_tag="PK389_04915"
     CDS             <1..1530
                     /gene="traN"
                     /locus_tag="PK389_04915"
                     /inference="COORDINATES: protein motif:HMM:NF018666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HQY23075.1"
                     /translation="VTCRKGVPPELRTFVKKLIVKAIPQPDLVKTVTAHFTAQCYNLV
                     TFNVNLKTGKISVSQCENQHHLNTSVDNSIGEPDYPDRTTISLISKEHFGEGGVDFRT
                     HQFNPAPSNGFTGSFTAFQPHTGRKKSHANNKNRVRGGRYVWKVTIPQKPTLQETWVG
                     CEDLERLQNENSCETVFSEQQNLNETRAIAGFETPVTRAHWLENKEFLCGGRDIDTCE
                     PYIQQKCEQIDSTCAVFERKTCVEYENTFRCNVSDYQKTDGISFNAGTLSFLPGTGQS
                     VTEGYNAVDFGEAVTGFSALIEMGKTLEDGLGGIVGDPNNPTVFRGQCRQCRVNLGSF
                     VRDCCKLKGVLQGLLGKCNEEEKKLAMAAVKNKRCIKIEGRYCHKKVAKVCVEKRDSY
                     CCYGSQLAKILHEIAHQQLGISMGSAEHPVCNGLTASQLSQLNFDTPFAQQKLSEILG
                     EVQASAQDKFNRVQQAVSAAKSLQLKAVDWEKKQAELQADVKHRAERQKQLSITAEQK
                     Q"
     gene            1527..>2033
                     /gene="traF"
                     /locus_tag="PK389_04920"
     CDS             1527..>2033
                     /gene="traF"
                     /locus_tag="PK389_04920"
                     /inference="COORDINATES: protein motif:HMM:NF025105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HQY23076.1"
                     /translation="MKTLNLLKMLSDLKTINVYLLKLCLFLLLPSLTFAQPFFEAHND
                     GWFWYQDFPIELSKNSAKRPLGDLSNPTEMMYDFRKKVEDSLNLAILSPTPENLRNYA
                     AQYFEVIRKGQRFTDAYKVMALNYPQYDYSLKFPTNHVAQSIHIAEELKIRQNKIHQF
                     SKSHGFFFF"
BASE COUNT          651 a          404 c          435 g          543 t
ORIGIN      
        1 gtcacctgta gaaaaggcgt tccccctgaa cttcgaacct ttgttaaaaa attaatcgtc
       61 aaagctattc ctcaacccga tttagtgaaa accgtaaccg ctcattttac ggcgcaatgt
      121 tacaacttgg tgacttttaa tgttaactta aaaacaggaa aaattagcgt atcgcaatgc
      181 gaaaaccaac atcatcttaa tacctctgtc gataattcta tcggagagcc ggattatccc
      241 gataggacaa cgattagctt aatcagcaaa gagcattttg gcgagggcgg tgttgatttt
      301 aggacgcatc aattcaatcc tgcgcccagt aatggtttta cgggttcttt tacggctttt
      361 cagccgcaca cgggcagaaa gaaatcccat gcaaataata aaaaccgcgt cagaggtggt
      421 cgttatgtat ggaaagtgac aatacctcaa aaacctacct tacaagaaac ttgggtaggg
      481 tgcgaagatt tagaacgcct acaaaacgaa aacagttgtg agacggtttt ctcagaacag
      541 caaaatctaa atgaaaccag agcaatcgca ggtttcgaaa caccggtcac aagagcgcat
      601 tggttagaaa ataaagaatt tctctgcggt ggtagagata ttgatacatg tgaaccttac
      661 atccaacaaa aatgcgagca aatagattct acgtgtgctg tgttcgagag gaaaacttgt
      721 gttgaatatg aaaatacctt tcgctgcaat gtgtccgatt atcaaaaaac agatggaata
      781 agttttaatg cgggaacctt aagctttttg ccaggaaccg gacaatcggt tactgaaggt
      841 tataatgccg tcgattttgg tgaggcggtc acaggcttca gcgcattaat cgaaatgggt
      901 aaaaccttgg aggacggatt aggcgggata gtaggagacc ccaacaatcc gactgtattt
      961 cgaggacaat gccgacaatg ccgcgtgaat ttaggttctt ttgtacgcga ctgttgcaaa
     1021 ctaaagggtg ttttgcaagg tttattgggt aaatgcaatg aagaagaaaa gaagctcgca
     1081 atggcggcgg ttaaaaataa gcgttgcatt aaaattgagg gtcgttattg tcataaaaag
     1141 gttgccaaag tctgtgttga aaaaagagac tcctattgtt gttatggttc acaattagcc
     1201 aaaatcctcc atgaaatcgc ccatcaacaa ttgggcattt cgatgggcag tgctgaacac
     1261 cctgtttgca atggtttaac cgcgagccaa ttaagccaat tgaattttga tacgcctttc
     1321 gcacaacaaa agctttctga aattttaggt gaggtgcagg cgagcgcaca ggataaattt
     1381 aatcgcgtac agcaggcggt gtctgcggct aaaagccttc aactcaaagc ggtcgattgg
     1441 gagaaaaagc aagcggaact tcaagctgat gttaaacatc gtgcggaaag acaaaagcaa
     1501 ctgagtataa ccgcagagca gaaacaatga agaccttgaa tttattaaaa atgctgagcg
     1561 atttgaaaac tattaacgtt tatttgttga agctgtgcct atttctactg ttaccatcgc
     1621 ttacttttgc ccagcctttt tttgaagcac ataacgatgg atggttctgg tatcaagatt
     1681 ttccgattga actgtctaaa aactctgcaa aacgccctct tggcgactta tctaatccca
     1741 cagaaatgat gtatgatttt cgaaaaaaag tggaagacag cctgaatctc gccattttat
     1801 cccctactcc cgaaaaccta cgaaattacg cagcgcagta ttttgaggtt attcggaaag
     1861 gtcaacgatt tactgatgcc tataaggtaa tggcacttaa ttatcctcaa tatgattaca
     1921 gcttaaagtt tccgaccaat catgtagccc aatctattca tattgcagaa gaactcaaaa
     1981 tcagacagaa caaaatccat caattctcaa aatctcacgg cttctttttc ttt
//
LOCUS       DAORVT010000223         2031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_59492_length_2031_cov_3.678138, whole genome shotgun sequence.
ACCESSION   DAORVT010000223 DAORVT010000000
VERSION     DAORVT010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59492_length_2031_cov_3.678138"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /gene="tolB"
                     /locus_tag="PK389_04925"
     CDS             <1..979
                     /gene="tolB"
                     /locus_tag="PK389_04925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HQY23077.1"
                     /translation="SFALLYIIRQKSEDALRSPLLAMHFDNVRSQDFRALAHHISDCI
                     FEKLIGIKGIFSTRIAYVSVTEGSKGTTHALEVADADGFNPKPLYRSAYPLMSPAWSP
                     DGKRIAFVSFEKNRAGINIVEVKSGHVERLTQFPGINGAPTWSPDNQTLALVLSKEGS
                     PKIYTMDVRSKRLSRLTDGASIDTEPSFAPDGRSIVFTSNRGGKPQIYRVALDSRKVE
                     RLSFKGDYNAKPSLTPDNKKLVMLHRGESGLFSIAVQTLNTGELKILTQSSLSDCPNL
                     APNGMMVIYGSSVGEQGVLGAVSLDGRVKIRLPSRQGNVQEPAWSPFVK"
     gene            1003..1518
                     /gene="pal"
                     /locus_tag="PK389_04930"
     CDS             1003..1518
                     /gene="pal"
                     /locus_tag="PK389_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HQY23078.1"
                     /translation="MRTKVKVFASVVLSSLLLLGLVGCGAKKGSGYDADGNIIGVNDT
                     VRFYGSDVSPAHEKDLLAKRTYYFGYDSFDVLDKDVLSVYAHAKRLINHKRMHVRVEG
                     HTDERGSREYNVALGERRANAIANILMLKGVPQDQISVVSYGKEKPAVAGHDESSWSQ
                     NRRAVIIYEVE"
     gene            1522..>2031
                     /locus_tag="PK389_04935"
     CDS             1522..>2031
                     /locus_tag="PK389_04935"
                     /inference="COORDINATES: protein motif:HMM:NF027655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgF trimerization domain-containing protein"
                     /protein_id="HQY23079.1"
                     /translation="MNWHSVWALAGLITFSVSLSVEALAIVESRTGEPIVYDAEEFVP
                     SPIARSSEDRATGSRVATNAPAEDRAALLSLKHLETLKQEVSELRGLVETQEHEIQQL
                     KKSQRDLYGDLDKRVTQLQSPKISAAAKKSSVTIVPTGTTSVSAVSAEKKIEKMTAEP
                     IASDFSDAAM"
BASE COUNT          565 a          360 c          520 g          586 t
ORIGIN      
        1 cagttttgca ctgttatata ttattcgaca aaaatctgaa gacgctctgc gctcaccgtt
       61 gttggccatg cattttgata atgtgcggtc gcaagatttt cgtgcattgg cgcatcatat
      121 cagcgattgt atttttgaaa aactgattgg tattaagggt attttttcaa cgcggattgc
      181 ttatgtgtct gtgacagagg gatctaaagg aacaacacat gcacttgaag tggcagatgc
      241 agatggattt aacccaaaac cgctgtatcg atctgcttat ccattgatgt cgcctgcttg
      301 gtcacccgat gggaaaagga tagcctttgt ttcgtttgaa aaaaatagag ctgggattaa
      361 tattgtagaa gtgaagagtg gacatgtaga acgcctaaca caatttccag ggattaacgg
      421 tgcgcctacg tggtctccag ataatcaaac tttggctttg gttttgtcta aagaggggag
      481 ccccaagatt tacactatgg atgtgcgcag taagcgactt agtcgtttaa ccgatggagc
      541 gagtattgat accgagccca gttttgcacc tgatggtcgt tcaatcgtat ttacatcgaa
      601 caggggtgga aagccgcaaa tttatcgtgt cgcattggat tctcgtaaag ttgagcgctt
      661 gtcatttaag ggtgactaca atgcaaagcc ctctttaaca cccgacaata aaaaattagt
      721 aatgttgcat cgcggtgaat ctggattatt tagcattgcg gtacagactt taaatacggg
      781 ggagttaaaa attttaacgc agtcgagtct cagcgattgt ccaaatcttg cgccaaatgg
      841 tatgatggtc atatatggat catcagtcgg ggaacaaggt gtgttaggag cggtgtcttt
      901 ggatggtcgt gttaagattc gcttaccttc tcggcaaggc aatgtgcaag agcctgcctg
      961 gtcccctttt gttaaatgac cttaagtttg taggagagag taatgcgtac taaagttaaa
     1021 gtgttcgctt cagttgtttt gtcgtcgttg ttattgttgg gattagtggg ttgtggcgct
     1081 aaaaaaggta gcggttacga tgccgatggc aatattatcg gcgttaatga taccgtgcgg
     1141 ttttatggtt cggatgtttc tccggcgcat gaaaaagatt tgttagcaaa gcgaacctat
     1201 tattttggtt atgatagttt tgatgtactc gataaagacg tcttgtctgt ctatgcacat
     1261 gcgaaacgtt taattaatca caaacgcatg catgtacgtg ttgaagggca tacggatgaa
     1321 cgaggtagtc gggagtataa cgttgcatta ggtgaaagaa gggctaatgc gatagcaaat
     1381 attctgatgc tgaaaggcgt tccacaagat caaattagtg tcgtgagcta tggtaaagaa
     1441 aagccagctg ttgcaggaca tgatgaatcg agttggagtc aaaatcgccg tgcggttatt
     1501 atttatgagg tagaatagag cgtgaactgg cattccgttt gggcattagc aggcttaata
     1561 acttttagtg tatcactttc tgtggaagcg cttgcaattg ttgagtctcg aacgggagag
     1621 cccattgttt atgatgctga agagttcgta ccttcaccga tcgcgcgatc ttctgaagat
     1681 cgcgcgacag gctctagggt cgcaacgaac gctccagctg aagatcgtgc tgctttactg
     1741 agtttgaaac atttagagac tttaaaacaa gaagtaagtg agttacgtgg cttggtcgag
     1801 acgcaagagc atgaaattca gcagctaaaa aagagccaac gagatctgta tggtgattta
     1861 gataaacgag taacgcaatt gcaatcgcct aaaattagtg ctgccgcaaa aaaatcttct
     1921 gtcacgattg tgccaacggg tacaacgtcg gtgtctgctg tatctgcaga aaagaagata
     1981 gaaaaaatga cagcagaacc gatagcttct gatttctcag atgcagctat g
//
LOCUS       DAORVT010000224         2012 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_60468_length_2012_cov_2.907511, whole genome shotgun sequence.
ACCESSION   DAORVT010000224 DAORVT010000000
VERSION     DAORVT010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60468_length_2012_cov_2.907511"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PK389_04940"
     CDS             complement(<1..345)
                     /locus_tag="PK389_04940"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HQY23080.1"
                     /translation="MAPEISLTFDMALVLGIIVFTVLLTLSNVIRVDVVAVLVLVVLG
                     ITRLLPPEQLFSGFSNDAVMSLISVMIIGAGLEKSGIALRIARWILKLGRERPNKINI
                     FLMFSSGFLSAFM"
     gene            complement(348..1046)
                     /gene="trmB"
                     /locus_tag="PK389_04945"
     CDS             complement(348..1046)
                     /gene="trmB"
                     /locus_tag="PK389_04945"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011707231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HQY23081.1"
                     /translation="MQVVSPYPLRPIRSFVRREGRISNAQLSAWERLWPIYGLSASDP
                     GCIAFESLFGRTADTVLEIGFGDGHSLAEMAKNAPEKNFIGIEVYRTGVGNLLTLIEK
                     HKLSNIRIFCADAVEILTNKIPDNSLTTLQLFFADPWPKVRHHKRRIVQASFIKLVSQ
                     KLKSLGTFHLATDWMDYAYHMMAVLGESVDFVNASGANQFTPRPDFRPVTKYEQRGLR
                     LGHQTWDLIFLNRD"
     gene            1154..1855
                     /locus_tag="PK389_04950"
     CDS             1154..1855
                     /locus_tag="PK389_04950"
                     /inference="COORDINATES: protein motif:HMM:NF014999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF234 domain-containing protein"
                     /protein_id="HQY23082.1"
                     /translation="MKFYNRSEELKVLDQNVILQKNSPFLNEGKNLLIEEFGRDYLTY
                     FTILELISLGKTSRTEMESLLGKDIGGYLQRLESDYAIVTRHRPIHAKPNTKNQKFKI
                     TDNFLNFWFRFIYRHQTAIEIDNFSYIKKIIKRDYSTYCGPILERFFQNLLAETRQFN
                     RIGSYWEKGNQNEINIVAINDMEKKILIGETKLNKSKISLKTLRVRAEPLLKEFPNYT
                     PTFLPLSIEDATSPF"
BASE COUNT          642 a          422 c          374 g          574 t
ORIGIN      
        1 cataaaggca gataaaaatc cagatgaaaa cattaaaaaa atattgattt tattggggcg
       61 ttctcggcct aattttagta tccatcgcgc aatccgcaat gcgataccgc ttttttctaa
      121 acccgcgcca atgatcatga cagaaatcaa cgacatgacg gcatcatttg aaaatcctga
      181 aaataattgt tccggtggca ataaccgcgt aatacccaaa acaactaaga ccaaaacagc
      241 aacaacatcg acgcgaataa catttgaaag cgttaacagt actgtaaata caataatgcc
      301 aagcactaaa gccatatcaa atgttaagct aatctcgggc gccactttta atccctattt
      361 agaaatatca aatcccaggt ttgatgaccc aatcgtaaac cgcgttgctc gtacttcgtg
      421 acaggacgaa aatctgggcg cggtgtaaac tgatttgcgc cactggcatt cacaaaatcc
      481 accgattctc cgagcactgc catcatatga taagcatagt ccatccaatc tgttgcaaga
      541 tggaaggtgc ccaggctttt taatttttgg gaaactaact taataaagga cgcctgcaca
      601 attcgtcgtt tatgatgccg caccttgggc cagggatccg caaaaaaaag ctgaagcgtt
      661 gttaagctat tgtcagggat cttgttcgtt aagatctcta cagcatccgc gcaaaaaatg
      721 cgaatgttgg atagcttgtg cttttcaatg agggtgagaa ggttgccaac gcctgtacga
      781 tagacttcta tgcctataaa atttttttca ggagcatttt tggccatctc tgctaatgag
      841 tgaccgtcgc caaagcctat ctcaagtacg gtgtctgcag tacgcccaaa aagactctcg
      901 aaggcgatac acccaggatc actggctgac aaaccataaa tcggccacaa tcgttcccag
      961 gcactcaatt gagcattgct aatcctgcct tcacgacgaa caaagctccg gatgggccgc
     1021 aggggatagg gtgatactac ttgcataatg ggcgcattct aacacattta ttgtctgtat
     1081 ttttaatcag tcattgaggt ttttcttttt tagtctatcg taaccatagt tacggtaacc
     1141 gtggttacgt tatatgaaat tttataatcg ctcggaagag cttaaagtgc tcgaccaaaa
     1201 tgtcatccta caaaaaaatt ctcctttttt aaacgaaggc aaaaacctat taattgagga
     1261 atttgggcgt gattatttaa cgtattttac catcttggag ctcatctctc ttggaaaaac
     1321 gtctcgaaca gaaatggaat ctttattagg aaaagatatc ggtggatatt tacaacgctt
     1381 agaatccgat tacgctattg ttacgcgaca tcgtcctatt catgcaaaac ccaacaccaa
     1441 aaatcaaaaa tttaagatta ccgacaattt tttaaatttt tggttccggt ttatttaccg
     1501 tcatcaaact gcaattgaaa ttgataattt ttcatatatt aaaaaaatta tcaaacgaga
     1561 ttactcaact tattgtggtc ctattttaga acgtttcttt caaaatctac tggctgaaac
     1621 aaggcaattt aatcgaatcg gttcttattg ggaaaagggc aatcaaaacg aaatcaacat
     1681 cgttgccatt aatgatatgg aaaagaaaat tttaattggt gaaacaaaat taaacaaatc
     1741 gaaaattagc ttaaaaacgc ttagggtgcg agcagaaccc ttattaaaag agtttcctaa
     1801 ttatacgccc acttttttac ctttaagcat tgaagatgca acatcccctt tttaaggctc
     1861 agcggatcca cgcgcacaga ggtattctct cgtattttga ccttgtgtta tgccgaaatg
     1921 acttgagctc gcaataacgt tcatattgtt acgcataggg gcaagaagcc taggaacatc
     1981 tccttcctct tgatttaaaa gcgtatttgc ac
//
LOCUS       DAORVT010000225         1998 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_61252_length_1998_cov_4.570252, whole genome shotgun sequence.
ACCESSION   DAORVT010000225 DAORVT010000000
VERSION     DAORVT010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61252_length_1998_cov_4.570252"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PK389_04955"
     CDS             <1..647
                     /locus_tag="PK389_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQY23083.1"
                     /translation="EIVTLIGPNGAGKSTLVKVVLGLMSPNKCTVQLEPGIRMGYMPQ
                     GLAIEPFMPLSVNRFLQLAENVSRHILNNTIDELTIRHLLAAPMQSLSGGERQRVLLA
                     RALLRKPDLLVLDEPVQGVDVTGQEELYRLVMHIRDTHHCGILMVSHDLYLVMAGTDS
                     VICLNKHVCCSGTPAAVSQHPEFLSLFGAWESGKLAVYAHQHNHKHNLGGDVCP"
     gene            638..1435
                     /locus_tag="PK389_04960"
     CDS             638..1435
                     /locus_tag="PK389_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HQY23084.1"
                     /translation="MSLDFMVRAVVAGMGIALVAGPLGSIIVWRRMANFGDALAHSTL
                     LGLCFALLLNIHIYIGLTSVCILVACSLALFSRQQHLANDTILCMLAYVTLAIGLILA
                     TVLKGIRVDLLGYLYGDILSVNNIDLIWIYCVDVIVFLSFVKIWRPILSMTVHEDLAK
                     VEGVPVFAMQWILVVIMAVVFSVAMKLVGILLITALLIIPASSARQISKTPENMAIIA
                     SIVGMVSVFGGIQISFNWDWPAGPSIVIVAMVLFVVSVFAARIRKIM"
     gene            complement(1472..1975)
                     /locus_tag="PK389_04965"
     CDS             complement(1472..1975)
                     /locus_tag="PK389_04965"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014409789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HQY23085.1"
                     /translation="MNDVKGSLDGKTLRKIADSGLKIAIMHSLTIPAQKDQCLDFEHV
                     PMESINQWAKQKIDYLEQFGFSKNDIILDPGIGFGKSSYQDISLFKSIHQLKDLGCEV
                     LVGHSRKAYTSSFYPSSANERDLETIAISSTLAAQGVDYLRIHHVKNHQRFFVAQQIS
                     QSQKVKE"
BASE COUNT          518 a          334 c          456 g          690 t
ORIGIN      
        1 gtgaaattgt aactcttatt ggacctaatg gagcgggtaa atcaacttta gtaaaagttg
       61 tgctgggctt aatgtcaccc aataaatgta cggtgcagtt agaaccgggc attcgaatgg
      121 gatacatgcc acaagggttg gctattgaac cgtttatgcc tttgtctgta aaccgatttt
      181 tacaactagc agaaaatgtc tcgagacata tattaaacaa cactattgat gaattaacca
      241 ttcgccattt gcttgctgcg ccgatgcaat cgctttcggg tggcgaacgt cagcgagtat
      301 tattagcgcg cgcgttgtta cgcaagcctg acctcttagt attagatgaa cctgtgcaag
      361 gtgtggatgt gacgggtcaa gaagaattgt accgtttggt tatgcatatt cgagacacac
      421 atcactgcgg cattttaatg gtctcgcatg atttgtattt agtgatggcg ggcacggatt
      481 ctgtgatttg cttgaacaaa catgtttgct gttcaggcac tcctgcagct gtgagtcaac
      541 atccagaatt tttaagtcta tttggggcat gggaatctgg aaaattagcc gtttatgcac
      601 atcaacataa tcacaaacat aatttaggtg gagatgtatg tccttagatt ttatggttcg
      661 cgcggtggtt gcgggtatgg gcatcgcatt ggtggcaggt ccgctgggct cgattattgt
      721 ttggcggcgt atggcaaatt ttggggatgc gttagcgcac tcaaccttgt tgggattatg
      781 ttttgcatta ttattgaata ttcatattta tatcggttta accagtgttt gtattctggt
      841 tgcgtgttct ttggcactgt tttctagaca acaacattta gcaaacgata caattctttg
      901 tatgttggct tatgtgacgt tggcgatagg gcttatttta gcaacggtat taaaaggcat
      961 tcgcgttgat ttgcttggat atttatatgg cgatattctt tcggtgaata atatagattt
     1021 gatttggatt tattgcgtcg atgttattgt gtttttaagt tttgtaaaaa tttggcgtcc
     1081 tattttatcg atgacggtac atgaagattt agccaaagta gagggggtac cggttttcgc
     1141 tatgcaatgg atattggtag tcattatggc agttgtcttt tcggttgcaa tgaaactagt
     1201 tggcatattg ttaattacag cgcttttaat tatcccggct tctagcgcga gacaaatttc
     1261 aaaaacaccg gaaaacatgg ctattattgc cagtatcgtt ggtatggtgt ctgtatttgg
     1321 gggcattcaa atctcattta attgggattg gccagcaggc ccctcgattg tcattgttgc
     1381 aatggttttg tttgtggtga gtgtgtttgc tgctcgcatt agaaaaatta tgtagtagtt
     1441 tgttctgttt taggccgttt aatatcgtct cttattcttt tactttttga gactgtgaaa
     1501 tttgttgtgc cacgaaaaag cgctgatgat ttttaacgtg atgaatgcgt aaatagtcaa
     1561 caccttgagc tgccaaggtg cttgaaatgg caatggtttc taaatcacgc tcatttgctg
     1621 aggatgggta aaatgaagaa gtataagctt ttctcgaatg accaactaat acttcgcatc
     1681 ccaagtcttt aagttgatga atgctcttga atagagagat atcttgataa gaagattttc
     1741 caaaacctat accaggatca agaataatat cgtttttact aaacccaaat tgttctaaat
     1801 aatcaatttt ttgttttgcc cattggttta tactttccat gggcacgtgt tcaaaatcta
     1861 aacattgatc tttttgtgcc ggaatagtca aggaatgcat aatagcgatt tttaacccgg
     1921 aatctgcgat ttttcttaac gttttaccgt ctaagctgcc tttaacatca ttgatccatg
     1981 agacaggata atccttta
//
LOCUS       DAORVT010000226         1994 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_61494_length_1994_cov_2.552347, whole genome shotgun sequence.
ACCESSION   DAORVT010000226 DAORVT010000000
VERSION     DAORVT010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61494_length_1994_cov_2.552347"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..1611)
                     /locus_tag="PK389_04970"
     CDS             complement(166..1611)
                     /locus_tag="PK389_04970"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HQY23086.1"
                     /translation="MIQPKRVLGTFTLAMITVAAIVSLRNLPLSAGFGLSSIFYFVVA
                     AGVFFIPIALTTAELATGWPRAGGNYVWVSEAFGKHLGFFSLWTAWMESIAWFPAILA
                     FTGAMLAHLLMPLFPGLENNKIFYFAVMLTVFWGATFLNFLGIKTSGWISALGVLTGT
                     LIPGALIIGLGVWWMIAGHPSALTFSWDALIPDFRLDSMVFFSGILLGLAGVEIAVFH
                     IREAKNPQKDYPKALAIASVLILSISILGTLAIAVVVPQTDISLLSGLIQAFKVFFEH
                     FELGWAVPIVALFLLIGSLAGVNTWTVGPAKGLLVSAEDGFLPPLLKRVNHAGVPTGM
                     LVFQALIGSILSLVFLWMDSNSAAYWVLTALSAQFTVVQYGIVFAAMLKLRYSQPNTP
                     RAFRIPGGKWGAWCVASIGIVACVFGFWIVYLPPSQLETGDRTIYQAMLAISFVLLSV
                     IPILFSIGNACKLAILTLADPSISLSSTAYK"
     gene            complement(1732..>1994)
                     /locus_tag="PK389_04975"
     CDS             complement(1732..>1994)
                     /locus_tag="PK389_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23087.1"
                     /translation="AGGAFVQSNLQMVFAATAISTGANAVSITAPYAFAVFLAVCVMW
                     LFAVKGLSKKVKAALDRRNQGTPEDDAPKAQDNLTTTKPQQA"
BASE COUNT          688 a          468 c          391 g          447 t
ORIGIN      
        1 gaaagatcaa agaaaagggt tcgttagtgc cactgatgac gatggttatt attataaaga
       61 agaaagcagt gatagttttg aaaaacaata ttctgatgat gacaaaataa aacctaaaaa
      121 aaggaattat gcaaaaggct tagaatctgt aacaagtgac gggtcctatt tgtatgccgt
      181 tgaagaaagt gaaattgatg ggtcggccag cgtaagaatt gcgagtttac aggcgttacc
      241 aattgaaaat aaaatgggaa taactgagag cagcacaaaa ctaatcgcta gcatcgcttg
      301 ataaatcgtt cggtctcctg tttctaattg cgatggaggc aaatacacta tccaaaatcc
      361 aaagacacag gcaacaatgc caatacttgc aacacaccaa gcaccccatt taccaccggg
      421 aattctaaaa gcccttggcg tattcggctg agaataacgt agctttagca tcgctgcaaa
      481 aacaatccca tactgtacca ctgtaaattg tgcagataac gccgttaata cccaatatgc
      541 tgcactgttg ctatccatcc acaaaaaaac cagtgataaa atagagccta ttaaagcctg
      601 aaagacgagc atacccgttg gcacacccgc atgattcacc cgctttaaca aaggcggtaa
      661 aaaaccatct tctgcagaaa ctaacagtcc ctttgcaggc ccaactgtcc aggtattaac
      721 acccgcaagc gaaccaataa gcaaaaacaa tgcgacaatt ggaactgccc agcctaactc
      781 aaaatgctca aaaaaaactt taaaggcttg gatcaaaccc gacaacaaac tgatgtcggt
      841 ttgcggaact acaacggcaa tcgctaaggt tcccaaaata gaaatcgata agataagaac
      901 agaggcaatc gctaaagctt tgggataatc tttttgcgga tttttagctt ctcgaatatg
      961 aaagaccgcg atttcgacac ccgccaagcc caataatatg cctgaaaaaa ataccatgga
     1021 atcgagacga aaatcgggaa ttaaagcatc ccagctaaaa gtcaatgcgc tcgggtggcc
     1081 tgcaatcatc caccaaacgc ccaagccaat aattaatgca cccggaatta aggtaccggt
     1141 taaaaccccc aacgcgctta tccagcctga agttttgatc cccaaaaaat tgagaaaagt
     1201 tgctccccaa aaaacagtta acatcacagc aaaataaaaa atcttgttgt tttctaaccc
     1261 tggaaataag ggcattaaca aatgtgctaa catagctccc gtaaaagcca agatggcggg
     1321 aaaccaggca atactctcca tccaagccgt ccacaacgaa aaaaaaccaa ggtgtttgcc
     1381 aaatgcttcc gacacccaca cgtagttacc cccagcacgt ggccacccag ttgccagttc
     1441 agcggtggtt aatgcgatgg gaataaaaaa tacccctgca gctaccacaa aataaaaaat
     1501 agaggataga ccaaaccctg cagataaggg taagtttcta agactgacaa tggctgccac
     1561 tgtgatcatt gccagggtaa atgtccctaa cacgcgtttt ggttgaatca aattaccccc
     1621 tcttcagtat tcaatgacaa actttacttc gtaaatgcta acaaatttgg gcgcaatagg
     1681 tgtacaacaa agaggctctc ccactcacgg gagagcctcg gagataagac cttaggcttg
     1741 ctgaggcttt gtggtcgtga ggttatcttg tgctttaggc gcatcgtctt caggtgttcc
     1801 ctgattacga cgatcaagag ctgccttaac ctttttggat aatcctttaa ccgcgaaaag
     1861 ccacataacg caaacagcaa gaaacactgc aaatgcataa ggtgctgtaa tagacacagc
     1921 attagcacct gtgctaattg ccgttgcagc aaacaccatt tgcaaattac tctgtacaaa
     1981 agcgccacct gctt
//
LOCUS       DAORVT010000227         1991 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_61634_length_1991_cov_4.189050, whole genome shotgun sequence.
ACCESSION   DAORVT010000227 DAORVT010000000
VERSION     DAORVT010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61634_length_1991_cov_4.189050"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..1199)
                     /locus_tag="PK389_04980"
     CDS             complement(96..1199)
                     /locus_tag="PK389_04980"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HQY23088.1"
                     /translation="MNPTDFSSTFKTSKTQKLRVKIGCYALLGATIHTCICLLIWIDG
                     FLHASGIEFISMFSVIWAGYLAYFILLKTGFNQSFKDPDLIMPIIVWAVTSVMYTVSL
                     TVEIRSLLLMFNLLILVFAAFNLNRRQYILVTLYGIVLYIGIIVYLKIFYPLKIDIKE
                     EGAVFLGYVLLTSALSAICYKISKLRKHLHQKNQKLAIAIKRIGAISMTDELTKIKNR
                     RYILDILRRQVLMVARGQYFFSVCMLDIDHFKQVNDVYGHLAGDSALKMLCQKVLTGL
                     REIDYFARIGGEEFLFVLPLVDKEQAKYMADRIRVQIENTNFDEVAPGLKMTISLGVS
                     DYQSPEKIETTLARIDSALYAAKRTGRNQVIVQ"
     gene            complement(1295..>1991)
                     /locus_tag="PK389_04985"
     CDS             complement(1295..>1991)
                     /locus_tag="PK389_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HQY23089.1"
                     /translation="FKDIASSPRGIVLVTGPTGSGKSTTLAAMVDYVNTTRSDHIITI
                     EDPIEFVHQSKKSLINQREVHRDTLGFKEALRSALREDPDVILVGELRDLETIRLALT
                     AAETGHLVFATLHTSSAAKTIDRVVDVFSGDEKEMVRGMLSESLRAVISQTLLKKDIG
                     GRVAAHEIMLCTPAIRNLIREAKVAQMYSAIQTGRAVGMQTLDQCLKALVEKGVINRK
                     EAKLKAENKELFS"
BASE COUNT          630 a          427 c          308 g          626 t
ORIGIN      
        1 aatgtgatca ctgtttttca acaacgcacc tccaaattaa tccactctca tggataatag
       61 aaaaaagaag aaattacttt gtgcacgttc ctcatttatt gaacgataac ttgattgcgc
      121 cctgttcgtt ttgcagcata taatgctgaa tcaatgcgag caagtgtcgt ttcaattttt
      181 tcgggacttt gatagtctga aacacctaat gatattgtca tttttaaacc gggagccacc
      241 tcatcaaaat tggtattttc aatttgaact ctaatgcgat ctgccatata ttttgcttgt
      301 tctttatcta ccaaaggcaa tacaaataaa aattcttctc ctccaattct tgcaaaataa
      361 tcaatttctc ttagccctgt taacaccttc tgacaaagca tttttaacgc tgaatcacca
      421 gccaaatgtc cataaacatc atttacttgt ttaaagtgat caatatctaa catacaaaca
      481 gaaaaaaaat attgtcctcg tgctaccatc aacacctgac gacgtaaaat atccaaaata
      541 taccgcctgt ttttaatctt ggttaattcg tctgtcattg aaatggcacc gatgcgctta
      601 atggctattg ccaatttttg atttttttga tgtaaatgtt tccttaactt actgatttta
      661 taacaaatag cagacagtgc ggaagtaagc aacacataac ccaggaatac tgctccttct
      721 tccttgatat caatttttag ggggtaaaaa attttcaggt aaacaataat gccaatgtat
      781 aatacaatgc catataaagt aacaagaata tactgacgtc tgtttaaatt aaaagcagca
      841 aatactaaaa tcaataaatt aaacattaaa agaagcgatc taatttcgac agttaaagag
      901 accgtataca taacagaagt taccgcccaa actataatcg gcattattag atcaggatct
      961 ttaaacgatt gattaaaacc tgttttaagt aatataaaat aagcaaggta ccctgcccaa
     1021 atgaccgaaa acattgagat aaattctatg ccagaagcat gcaaaaaacc gtcgatccaa
     1081 atcaacaaac agatacatgt atgaatcgtc gcgcccagca atgcataaca tcctattttt
     1141 actcggagtt tttgcgtttt gctcgtctta aacgtgcttg aaaaatctgt aggattcatt
     1201 agaaaactca tcttaacctt ttaacaatgt gactaatttt ctgtttggat gctttttttc
     1261 taccacctgg ttaaaccatg cctctaaatt cattttaact gaataattcc ttgttttctg
     1321 cttttagttt agcttctttt cgattaataa cgcctttttc tactagtgct ttcaaacatt
     1381 gatcaagggt ttgcatgcca acagctctgc ccgtttgaat ggcagaatac atttgagcca
     1441 ctttggcctc gcgtattaaa ttacgaatag ccggtgtgca cagcataatt tcatgagcag
     1501 ccactctgcc tccaatatct tttttgagta gtgtttgcga aataactgct cgtagcgatt
     1561 ccgacaacat accacgtacc atttcttttt catcacccga aaaaacatca accacccgat
     1621 cgatagtttt tgcggcagac gaggtatgta gcgtagcaaa cactaaatgt ccggtttctg
     1681 ctgctgttaa cgccaaacga atggtttcga gatcacgcaa ttcccctacc aaaataacgt
     1741 caggatcctc acgcaacgcc gaacgcagtg cttctttaaa tccaagcgta tcgcgatgta
     1801 cttctcgttg attaataaga cttttcttgc tttgatgaac aaattcaata ggatcctcaa
     1861 tcgtaataat atgatcactg cgcgttgtat tcacataatc taccatcgcg gccaatgtcg
     1921 tgcttttccc agaacccgtt ggacctgtta ctaatacaat tccccgtgga ctgctggcaa
     1981 tatccttaaa a
//
LOCUS       DAORVT010000228         1984 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_62045_length_1984_cov_3.162260, whole genome shotgun sequence.
ACCESSION   DAORVT010000228 DAORVT010000000
VERSION     DAORVT010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62045_length_1984_cov_3.162260"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(240..315)
                     /locus_tag="PK389_04990"
     tRNA            complement(240..315)
                     /locus_tag="PK389_04990"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(280..282),aa:Ala,seq:ggc)
     gene            complement(401..1783)
                     /gene="gltX"
                     /locus_tag="PK389_04995"
     CDS             complement(401..1783)
                     /gene="gltX"
                     /locus_tag="PK389_04995"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018946981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HQY23090.1"
                     /translation="MTIRTRFAPSPTGFLHVGSARTALYSWLYAKKMGGQFILRIEDT
                     DQERSTTEAVDAILEAMDWLGLDYDEGPFYQTKHFDRYREAADQLLKTDCAYYCYCSK
                     ERLEKLREEQTLNKEKPRYDGHCRDLRLSVRNEPHVIRFRNPEHGSVVIDDCVRGRVV
                     IQNSELDDLIIVRSDGTPTYNFTVVVDDWDMKITHVVRGDDHLNNTPRQINILKALGA
                     HLPIYAHIPMILGSDGKRLSKRHGAVSVQQYRDDGILPEALLNYLVRLGWSHGDQEIF
                     TREEMIQYFDIHDINKAPSAFNPEKLLWLNQNYLKTGDSTLLTKHLDWHMHHLAVDTD
                     EGPALEMVLLAQRDRAKTLKEMAEKSLFFYQEIDIDLEAREKHLTPEALEHLRELQAV
                     LAEISDWQAPVIHQEIEKLAERLEIKLGKLAQPLRVAVTGNTTSPPIDITLQLIGKRR
                     VLERLKIALL"
     gene            1902..1978
                     /locus_tag="PK389_05000"
     tRNA            1902..1978
                     /locus_tag="PK389_05000"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1936..1938,aa:Met,seq:cat)
BASE COUNT          562 a          450 c          365 g          607 t
ORIGIN      
        1 agcccctttt catcagccat tttaaatggc ttatcggcgg gctttttgtt tttaatcgtg
       61 aagtcattta gtgccatcag gggtatctcc cataccaaca gcttgatata cccctatttg
      121 taccccaaat acaggctgga tgttgggcat attaggatgt ggaaatacaa gaaaaggctt
      181 tttttacaga gttttttata cttctttaga ttttctggga tgtcttagga aggggtgatt
      241 ggtggagcta ggcgggattg aaccgccgac ctcttgcatg ccatgcaagc gctctcccag
      301 ctgagctata gcccctattt agcacataat gcgcttatga tagaggtcga atcctacagt
      361 ccaagaacgc tcctgtcaag cccctcacaa caacagcccc tcacaacaaa gcaattttca
      421 ggcgctccaa tacgcgcctc ttaccaatca gctgcaatgt gatatcaatt ggcggagagg
      481 ttgtattacc tgttacagcg acgcgcaatg gctgcgcaag cttgccaagt tttatctcta
      541 aacgctcagc cagcttttca atttcttgat gaataaccgg tgcctgccaa tctgaaattt
      601 ctgccaacac tgcctgtaat tctcgaagat gctccagcgc ctctggcgtt aagtgttttt
      661 cacgcgcttc taaatcaata tcaatctctt gataaaaaaa taagcttttt tccgccattt
      721 cttttaaagt tttcgcgcga tcgcgttgcg ctagtaagac catttctaat gcaggacctt
      781 catcagtatc aacagccaag tgatgcatat gccaatctaa atgttttgtg agtaaagtag
      841 aatcgcctgt tttcaaataa ttctgattta accacaataa tttttcaggg ttaaatgcag
      901 aaggcgcttt attaatatca tgaatatcaa aatattggat catttcttct cgagtaaaga
      961 tttcttgatc gccatgagac caccctaaac gtaccaaata atttaataaa gcttctggca
     1021 aaataccatc atcgcggtat tgttgcacac taactgcacc atgtcgttta gacaatcgct
     1081 tgccatcgct acctaaaatc ataggaatat gtgcgtaaat aggtaaatgt gcccccaatg
     1141 cctttagaat atttatttgt cgcggcgtat tatttaagtg atcatcccct cgtacgacat
     1201 gggtaatttt catatcccaa tcatctacga ccaccgtaaa attatacgta ggtgtcccat
     1261 cagagcggac aataatcaga tcatccaact ctgaattctg aatcaccact cgaccacgaa
     1321 cacaatcatc aatcacaaca ctaccgtgtt cgggattacg aaatcgaata acatgcggtt
     1381 catttcgaac agataacctt aagtctcgac aatgaccatc ataacgcggt ttttccttgt
     1441 ttaacgtctg ttcttcacgt aatttttcta aacgttcttt agagcaataa caataataag
     1501 cacagtctgt ttttaataat tgatctgcag cttctcgata acgatcaaaa tgctttgttt
     1561 gatagaaagg gccttcatcg taatctagcc ctaaccaatc catggcttct aatatggcat
     1621 caactgcctc agttgtcgat cgctcttgat ccgtatcttc gatgcgtaaa ataaattgtc
     1681 cacccatttt tttggcatac aaccacgaat aaagtgcggt gcgagcacta ccaacatgta
     1741 aaaatcctgt ggggctaggc gcaaagcgtg tacgtattgt cattgaaata accttctgtc
     1801 tttgaatcaa aagtaaattg atttcatact gtgtacaatg ctaccatatc ttgcaataca
     1861 acgcatttga gttagttgtt ttaatttttt agagttcttt tggctatata gctcagttgg
     1921 ttagagcgcg gcactcataa tgctgaggtc ggtggttcga gtccacctat agccaccaat
     1981 taaa
//
LOCUS       DAORVT010000229         1979 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_62322_length_1979_cov_3.584200, whole genome shotgun sequence.
ACCESSION   DAORVT010000229 DAORVT010000000
VERSION     DAORVT010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62322_length_1979_cov_3.584200"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            371..1066
                     /locus_tag="PK389_05005"
     CDS             371..1066
                     /locus_tag="PK389_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HQY23091.1"
                     /translation="MSVLTMVRKLLLPLAAAIGFIFGENVMAIEEPGFERLEKIENFE
                     IRQYRPVVVAETFVDGDLSSASNIGFRRIADYIFGNNRSMLGTVSKPSEKIAMTAPVL
                     VEPVERSEKIAMTAPVSVEPQGDSASGVMQSNRWRVQFTMPSEYSKATLPTPVNPEVS
                     LRDVPGKRFAVNVFSGFSGEERVQQKIDELFVWLDGKNIKSIGAPQLARYNPPWTLPF
                     WRRNEILVEIAAP"
     gene            1176..1400
                     /locus_tag="PK389_05010"
     CDS             1176..1400
                     /locus_tag="PK389_05010"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HQY23092.1"
                     /translation="MEPVWAIPCYVAGITSEIDGSELPQYHLGERVLDESEYLLMSDV
                     NPNSFDARYFGLIAGLQIQHVVEPFFTWGK"
     gene            1449..1594
                     /locus_tag="PK389_05015"
                     /pseudo
     CDS             1449..1594
                     /locus_tag="PK389_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918191.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          558 a          364 c          451 g          606 t
ORIGIN      
        1 aaagtgggcg gattggttgg aagatcgatt cctttcgtca aatacccata atcagtaaga
       61 attcttttcc acaaaatctg tggataaagc tgtagatgga tgtgctaagt atatggggat
      121 aatagactca taattattgc cttaaatgaa ggcaggaatt ttttctgcac gataaggtac
      181 attaattaaa tccatttctc tgtacgtagc tgcacaccca ttgaaaccag gtttacatac
      241 tatgccgcat gccgccggcg caccgttttt tgtagttata ggtgcgatgc cgtactgaat
      301 tgagataaga tttaacttaa tattgacttt ggtcagtgct ttacactgca cacttcaacg
      361 aggaaaaacc gtgagcgtat tgacgatggt tcgcaaactt ctgctgccac tagcggcagc
      421 gattggattc atttttggcg agaacgttat ggcaatagaa gaaccaggtt ttgagcgatt
      481 agaaaagatc gagaatttcg agattcggca atatcgtcca gttgttgtgg cggaaacttt
      541 tgttgacggc gatttgagtt cggcttctaa tattggtttt cgacggatcg ccgattatat
      601 ttttggcaac aaccggtcca tgctcgggac ggtgagcaag ccatcagaaa aaattgcaat
      661 gaccgcaccg gtattggtag agcctgtcga gcgttcggaa aagatcgcca tgactgctcc
      721 tgtgagcgtt gaaccccaag gtgattccgc tagtggggtg atgcagagta atcgttggcg
      781 cgtccagttt acgatgccca gtgaatattc aaaggcaacc ctcccaacgc cggtgaatcc
      841 tgaagtttca ttaagggacg tgccaggcaa acgctttgcg gttaatgttt tttcgggatt
      901 ttctggcgaa gagagggtgc aacagaaaat cgatgagctt tttgtttggc tcgatggaaa
      961 aaacataaaa agtatcggtg cgccccaact agcgcgctac aacccgcctt ggacattgcc
     1021 gttttggcga aggaatgaaa ttctggtgga aattgcggcg ccctgattta tgaggtttta
     1081 gcgggtttta aattgagagc agattaaaac tatttgccgc agcagtttat tttcctgaat
     1141 tgccccaact ccgcacgcag ttcattataa agattttgga gccagtctgg gccattccat
     1201 gttacgtagc tggaattact tcggaaattg atggttctga attgccgcaa tatcatttgg
     1261 gtgagcgggt gctggatgaa tctgaatatc tgctcatgtc cgatgtaaat ccgaactctt
     1321 ttgatgcgcg gtattttggg ctgattgcgg gtttgcagat tcaacatgtt gttgagccgt
     1381 tttttacttg gggtaagtaa aagtactgcc atgttaactg tatctgagcg acaatagcag
     1441 aatgaaaaat tcgggggccc caatacaaac gctacagatt tccaccagaa atcattcaat
     1501 acacagtgtg gctctattat cggtttaatt taagtcacca tgatatcgaa gacttgttag
     1561 cttaaaagga atcatagtga cccatgaatc tatttgtcta tgtcgtaatt ccaattctcg
     1621 ataaacatgc aaattaataa aatatataaa taactgttta taattattgc tatttatatc
     1681 tgtaattgga gtaataagct tggtttagca tatgccaaga agctaaggtg cccacatcta
     1741 ggatacggtg atactttttt tcttgatgaa gtattcgtaa agattcaagg caaacaacta
     1801 tatttgtgca ttggtgtccc attaactatc gcttgaaaaa agccatcttt tccctattta
     1861 acaatcagat aagaataagg ctatcatcaa atatttgatt ttaactattt tgaggttaat
     1921 tttgtgaaat gtggaatctt tgcgatctca tattgttata gtcaattgta atttatgaa
//
LOCUS       DAORVT010000230         1967 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_62988_length_1967_cov_3.005753, whole genome shotgun sequence.
ACCESSION   DAORVT010000230 DAORVT010000000
VERSION     DAORVT010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62988_length_1967_cov_3.005753"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="PK389_05020"
     CDS             <1..571
                     /locus_tag="PK389_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002707957.1"
                     /note="This protein performs the mismatch recognition step
                     during the DNA repair process; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HQY23093.1"
                     /translation="MITGPNMGGKSTYMRQNALIVLLAHIGSFVPAQSAKIGKFDKIF
                     TRIGAQDDLSSGRSTFMVEMTEAALILQNATAQSLVLLDEIGRGTGTFDGLSLAWAIA
                     SYLTTEIKAFTLFSTHYFEMTELPKSYSTIANVHLDAVEFGESLVFLYSLQEGPASQS
                     YGIQVAQLAGVPASVITIAKQKLLSLESN"
     gene            complement(581..997)
                     /locus_tag="PK389_05025"
     CDS             complement(581..997)
                     /locus_tag="PK389_05025"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HQY23094.1"
                     /translation="MKSKKGRDPVRPIEPIEPTGELVIQTVAMPADANAYGDIFGGWL
                     LSQMDVGGAVLAHQRAHNRVATVAIDKLTFMKPVYVGDLVCCYAKTFKTGHSSITIQL
                     QVFASRMRMGAHEQIAEGLFTFVAIDDNGRPKSINW"
     gene            complement(1011..>1967)
                     /locus_tag="PK389_05030"
     CDS             complement(1011..>1967)
                     /locus_tag="PK389_05030"
                     /inference="COORDINATES: protein motif:HMM:NF015148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HQY23095.1"
                     /translation="IDQTVFWGMIVFATIFSLIVAFIAAAIAAYITGIVGTTALPISG
                     ITIFAIITFASILLIILRPYIDFELNTDLALKAAAIVIVFSAIICVAGAVSGDNMQDL
                     KAGQLVGATPWKQQLMLVVGAVAAALVIPFILQTTFEAYGIGDVLPRAGMNPEHALPA
                     PQATLMATVAKGFFIGQLPWNMILIGVALAVVAIIFDTYLKKTNSTYRFHPLLFALGI
                     YFPLSYVTAFFMGGLVRSLANWGKKSKDDASETNHGILCASGIIAGEAILGALLTIPF
                     AYYQSTDIFALKVEWLTRHETLIGVFLYVGLCAYLYKRGRKA"
BASE COUNT          624 a          445 c          367 g          531 t
ORIGIN      
        1 gatgattaca ggccccaata tgggtggaaa atcgacttat atgcgacaaa acgcacttat
       61 cgttttgctt gcgcatattg gcagttttgt gccagctcaa tccgctaaaa ttggtaaatt
      121 tgataaaatt tttactcgca ttggtgcaca agatgatctc agtagtggtc gctctacttt
      181 tatggtagaa atgaccgaag ccgcacttat actacaaaac gcaacagcgc aaagtttagt
      241 cttattagat gaaatcggcc gaggcaccgg cacttttgat ggtttgtctt tagcatgggc
      301 tattgcctct tacttaacaa cagagattaa agcttttacc ttattttcga cccactattt
      361 tgagatgaca gaattgccga aatcgtattc gactatcgca aatgttcatc tagatgccgt
      421 cgaatttggt gaatcactcg tatttttgta tagcctacaa gaaggtccgg ctagccaaag
      481 ttatggcatt caggttgcac aattagcggg ggttcctgcc tctgtcatta ccattgcaaa
      541 acaaaaatta ttatcgctag aatccaacta aaggcgtttt tcaccaatta atagacttag
      601 ggcgtccatt gtcatctatt gcaacaaagg taaaaagccc ttctgcgatt tgttcatgcg
      661 cgcccatgcg catccggcta gcaaagactt gtaattgaat cgtgatagaa gaatgacctg
      721 ttttgaaggt ttttgcataa caacaaacca aatcgcccac ataaaccggt ttcataaaag
      781 tgagcttatc gatggcaact gttgccactc gattgtgcgc acgttgatgt gccaatacag
      841 ctccaccaac gtccatttga gacaacaacc atccaccaaa aatatcacca taagcattcg
      901 catctgctgg catcgcgacg gtttggatca cgagttcacc agtaggttct ataggctcta
      961 taggtcttac tggatctcgc ccttttttgc tcttcatttc aaaaacccgc ctacgctttt
     1021 ctgccacgtt tatataaata ggcacataat cccacataga ggaatacgcc aattaaggtt
     1081 tcgtgacgtg ttaaccactc cacttttaat gcaaaaatat cggttgattg atagtaggca
     1141 aacggaatgg tcaacaaagc acctaaaatg gcttctcctg cgataatacc cgatgcacat
     1201 aaaataccat ggttggtctc agaagcgtca tctttagatt tcttgcccca attggctaaa
     1261 gaacgaacca atccacccat aaaaaatgcc gttacataac ttaaaggaaa gtaaatgcca
     1321 agcgcaaaca ataaagggtg aaagcgataa gtactattcg tttttttcaa ataggtatca
     1381 aaaataatcg cgacaaccgc taaggctaca ccaattaaaa tcatattcca aggtaattga
     1441 ccaataaaaa aacccttagc aaccgttgcc attaatgtcg cttgtggcgc gggcaacgca
     1501 tgttcagggt tcatccctgc gcgtggtaaa acatccccta taccataagc ctcaaaagtg
     1561 gtctgcaaaa taaaagggat aactaatgca gcagcaacag cacctacgac taacattaac
     1621 tgttgtttcc aaggcgttgc gcctaccaac tgtccagcct ttaagtcttg catattatca
     1681 ccactcacag cgcctgccac acaaataata gcggaaaata caatcacaat ggcagccgct
     1741 tttagcgcta aatctgtatt tagctcaaaa tcaatgtatg gtcttaagat aattaataaa
     1801 atactcgcaa aagtgataat agcaaaaata gtaatgcctg aaatcggtaa cgctgtggtt
     1861 cccacaatac cggttatata cgccgcaata gcagcggcaa taaaagcgac tattaatgag
     1921 aagattgtcg caaaaacaat cataccccaa aatactgttt gatcaat
//
LOCUS       DAORVT010000231         1961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_63336_length_1961_cov_3.239769, whole genome shotgun sequence.
ACCESSION   DAORVT010000231 DAORVT010000000
VERSION     DAORVT010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63336_length_1961_cov_3.239769"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /gene="ampD"
                     /locus_tag="PK389_05035"
     CDS             complement(<1..300)
                     /gene="ampD"
                     /locus_tag="PK389_05035"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HQY23096.1"
                     /translation="MEIDVATGMLCDVRYVPSPNYNERPTDTVIDLLVIHNISLPPGE
                     FGGPHIDAFFTNVLNPSLHPYFAEISGLEVSSHCLIRRDGEIVQYVPFNKRAWHAG"
     gene            564..1136
                     /locus_tag="PK389_05040"
     CDS             564..1136
                     /locus_tag="PK389_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HQY23097.1"
                     /translation="MLIFAILIACIFALFTYLIVMYNNLISLKNNVSKNWSNIDVLLK
                     QRYSELPKLIDTCKEYMAYEKDTLEKIVVARKAALFASDTRDLIALGQAENSLKTGLT
                     QLFALAENYPNLKTNESFQRLEQRISELESSIADRREFYNDSVNLNNIRIQQFPDILV
                     ARAFNFRAFDLLKFNETEIQDVGIKKQFKT"
     gene            1136..>1961
                     /locus_tag="PK389_05045"
     CDS             1136..>1961
                     /locus_tag="PK389_05045"
                     /inference="COORDINATES: protein motif:HMM:NF023899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006996 - organelle organization [Evidence
                     IEA];
                     GO_process: GO:0016567 - protein ubiquitination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIDE domain-containing protein"
                     /protein_id="HQY23098.1"
                     /translation="MDLDTVLTLIFVCLPIFFALICFYYAIYFVKAARNIEDSPTSKI
                     RSAAQGYVELKGTPKPFPNYPTIGKLTQKPCAWYRYTVEIFQTIRTPSQTNGWRLLDQ
                     GMSQALFLLDDGTGQCVIQPWGAEIMPTQISWRGHTRIPSPPSTSFLRWFFWDSWGSY
                     RYTEYRLEFGMPIYASGMFRTLSSTDPRIQDALLLKSYLTQNNLYDFNFLSKEDLVGN
                     QNCIISAIPESRVIRQFKIKAFIFFIVFVFFSIVSVHSSYPIVKKSFQDWKKYKPPL"
BASE COUNT          629 a          415 c          337 g          580 t
ORIGIN      
        1 tcccgcatgc cacgcacgtt tgttaaaagg aacatattga acgatctcgc catcacgccg
       61 gatcaaacag tggctcgata cttctaaacc agaaatctct gcaaaatagg ggtggagcga
      121 tggatttaaa acatttgtaa aaaaagcatc aatatgcggt ccaccaaatt cacctggcgg
      181 caaactgata ttatggataa ccaggagatc gatgactgta tcggttggac gctcgttata
      241 attcggtgaa ggcacatatc gtacatcgca cagcatgcct gttgctacat caatttccat
      301 gttattttta ttccacgcta aatgcgacat catagcgcga accagctccc taatacatac
      361 cgacaaaaaa agttaataaa cttacataaa cgcatctgtc tttctctact cctatctttg
      421 gtcattttac cctttggtaa atacgataac aaacataccg tgacaaaaca gcgatccccg
      481 catagaatca aagatatggc tttataatta tctgaaaagc tataggatac aatggccagg
      541 gcaaaaaagc caaggaacca actatgttaa tattcgccat tcttattgca tgcatatttg
      601 ccttgtttac ttatcttatt gttatgtata acaatcttat ttcacttaaa aataatgtct
      661 ctaaaaactg gtctaatatt gatgtgcttt tgaaacaacg ctatagcgaa ttacctaaat
      721 taattgatac atgtaaagaa tatatggcat atgaaaaaga caccttggaa aaaattgtag
      781 tcgccaggaa ggcggcactc tttgctagtg atacaagaga tttgattgca ctcggacaag
      841 cagaaaattc tttaaaaact ggcttaaccc aattgtttgc tttagcagaa aactatccca
      901 atcttaaaac gaatgagtct tttcaacgat tagaacaacg gatcagtgaa ttagaatcca
      961 gcattgccga tcgacgagag ttttacaatg atagcgttaa tcttaataat atccgtatcc
     1021 aacaatttcc agatatcttg gtagcgcgcg cctttaactt tagagcattc gatttattaa
     1081 aattcaatga aaccgaaata caagatgttg gtataaaaaa acagtttaaa acctaatgga
     1141 tctggatact gtattaactc ttatttttgt ctgtctgccc attttttttg cgctgatttg
     1201 tttttattac gcaatctatt ttgtcaaagc cgccagaaac attgaggatt ctccaacctc
     1261 taaaattcgt tctgcagcac aaggctacgt ggaattaaaa ggcacaccaa aaccatttcc
     1321 aaattacccg acgataggta aactaacgca aaaaccatgt gcctggtacc gatataccgt
     1381 tgaaatattt caaactatca gaacgccctc acaaacaaac ggttggcgtc tactcgacca
     1441 gggcatgtct caggctcttt tcctactaga cgatggaacg ggacaatgcg ttatccaacc
     1501 gtggggagcc gaaattatgc ccacgcagat ctcttggcgg ggtcacacac gaatcccaag
     1561 tccaccttca acttcttttt tacgatggtt tttttgggac agctggggtt cttaccgtta
     1621 tacggaatat cgcttggaat ttggaatgcc aatctacgct tccggcatgt tccgcacatt
     1681 atcatcaacg gatccgcgca ttcaagatgc gttactatta aaaagttatc taacacaaaa
     1741 caatctctac gacttcaact tcttgtcaaa agaagactta gttggaaatc aaaattgtat
     1801 tatatctgct ataccagaat cccgtgttat cagacaattt aaaataaagg cctttatctt
     1861 ttttatcgtg tttgttttct tttcaatagt atcggttcat tccagttacc ctattgttaa
     1921 aaagtctttt caagattgga aaaaatataa accacctctg c
//
LOCUS       DAORVT010000232         1960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_63390_length_1960_cov_3.522835, whole genome shotgun sequence.
ACCESSION   DAORVT010000232 DAORVT010000000
VERSION     DAORVT010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63390_length_1960_cov_3.522835"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="PK389_05050"
     CDS             complement(<1..339)
                     /locus_tag="PK389_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HQY23099.1"
                     /translation="MLKIYNTLSKQKEIFKPLQPGRIDIYVCGVTPYDYCHIGHGRVF
                     LIFDVVIRYLRSISFEVNYVRNITDIDDKIINRAQEKNISFSALTAEFIQAMNTDFQH
                     LNILPPTAEPR"
     gene            complement(323..1732)
                     /gene="gltX"
                     /locus_tag="PK389_05055"
     CDS             complement(323..1732)
                     /gene="gltX"
                     /locus_tag="PK389_05055"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HQY23100.1"
                     /translation="MNTSLKTRFAPSPTGLMHFGNLRTALFNYLYARHYNGSFLLRIE
                     DTDMARSEAQYTDAILTDLRWLGLEWQEGPYHQSERQAIYNDYYEALEKKGRVYPCFC
                     TEEQLAITRKVQLASGKPPRYSGTCRHLSQEAILAKRASGLSFTLRFEVSNDAVVEFE
                     DLVKGPQRFETEHIGDFIIRRNDGTASFMFCNALDDALLGVTHALRGDDHLTNTPRQI
                     LILQALQLPTPRYGHFPTILGPDSKPLSKRNGSRAIQDLREEGFLPLGILNYLARLGH
                     HDAEQSLLNLDTLCAHFDLAHISNSAAHYDAVQLNHWQKEAMHRSTSVECWQQIAPYV
                     AERVPEAKVSAFVETVQQNLVMPKDALQWANAIFTDAAALHYSEAVSGVIFDAGSHFF
                     EHALFFINKMGSELTFPSLVSELQQKTGLKGKALFFPLRAALTAELHGPELAKVLDLM
                     GLESAKARLKRAENHAKNL"
     gene            1822..>1960
                     /locus_tag="PK389_05060"
     CDS             1822..>1960
                     /locus_tag="PK389_05060"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQY23101.1"
                     /translation="MNIVKLHTSLGTITIELDDKATPETAQNFLNYVNAGFYNNTIFH
                     RV"
BASE COUNT          589 a          447 c          368 g          556 t
ORIGIN      
        1 tcgaggttcc gctgtcggag gcaggatatt taaatgctga aaatccgtat tcatggcctg
       61 gataaattct gctgttaacg cactaaaaga aatatttttt tcttgagccc tgttaataat
      121 tttatcgtct atatctgtaa tgtttcgaac ataattcact tcaaaactaa tagaacgcaa
      181 atagcgtatc acaacgtcaa atattagaaa aacccggcca tgtccgatat ggcaataatc
      241 atacggcgta acgccacaga cataaatatc tattctaccc ggttgtaagg gcttaaagat
      301 ttctttctgc ttgctcagag tattataaat ttttagcatg attttctgct ctttttaatc
      361 tggctttagc cgattctaat cccatcagat ctaacacttt tgctaactcc ggcccatgca
      421 gttctgccgt taacgcagcc cttaagggga aaaagagggc tttacccttc agacctgttt
      481 tttgttgtag ttcactcacc agtgaaggaa acgtcaattc agaacccatc ttattgataa
      541 agaataacgc atgctcaaaa aaatgagatc cggcatcgaa gataacccca ctgactgctt
      601 cagaataatg taacgccgcg gcatctgtaa aaatcgcatt cgcccactgc aaagcatctt
      661 taggcataac cagattttgt tgtaccgttt ctacaaaggc tgacactttc gcctctggca
      721 cacgctctgc cacatagggc gcaatctgtt gccaacattc taccgaagta ctccgatgca
      781 tcgcttcttt ctgccaatgg ttaagttgta cagcatcata atgcgcagcg gaattactaa
      841 tatgcgctaa atcaaaatgc gcacaaagag tgtctaagtt taatagcgat tgctctgcat
      901 cgtgatgccc taaacgcgcc aaataattta agatccccaa tggcaaaaaa ccttcttctc
      961 gtaaatcctg aatcgctcta ctgccattgc gcttcgataa aggtttgcta tccggaccca
     1021 aaatggtcgg aaaatgtccg taacgtggtg taggcaattg caaagcttgt agaattaaaa
     1081 tctgtctcgg cgtattagtc aggtgatcat cccctcgcaa ggcatgcgtc acccctaata
     1141 aagcatcgtc taaagcatta caaaacataa atgacgccgt accgtcatta cgtcgaataa
     1201 taaaatcacc aatgtgctcg gtttcaaagc gttgaggacc tttcaccaaa tcttcaaatt
     1261 caacgaccgc atcgttggag acttcaaatc gtagcgtaaa cgacaagccc gaagcccgtt
     1321 ttgctaaaat agcttcttgc gatagatgtc gacaggttcc tgaatagcga ggtggtttac
     1381 ccgatgccaa ttgaaccttt cgggtaatcg ctaattgctc ttctgtgcaa aagcagggat
     1441 atacacgacc ttttttttcc agggcttcgt aataatcatt ataaatagct tggcgttctg
     1501 actgatgata aggtccttct tgccattcta gaccaagcca acgcaaatcc gtcaaaatgg
     1561 catcggtata ttgcgcttca gagcgagcca tatcggtgtc ctcaatgcgc aataaaaagc
     1621 tgccgttata gtgcctagcg taaagataat taaacaaagc agtccgaaga ttgccaaaat
     1681 gcataagccc tgtggggctt ggcgcaaaac gcgtttttaa agaagtattc attctttgat
     1741 tgtacacaat ttagctctta tccaacacta tctatatatg gtaaactaag gcttttaacc
     1801 ctgaacaagg acaaaaatgt aatgaatatc gttaaactgc atacttcctt aggcaccatc
     1861 acaattgagt tagatgataa agcaactccc gagaccgcac agaattttct gaactatgtt
     1921 aacgcaggtt tttataacaa cactattttt catcgtgtta
//
LOCUS       DAORVT010000233         1952 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_63857_length_1952_cov_3.150237, whole genome shotgun sequence.
ACCESSION   DAORVT010000233 DAORVT010000000
VERSION     DAORVT010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63857_length_1952_cov_3.150237"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..586
                     /locus_tag="PK389_05065"
     CDS             41..586
                     /locus_tag="PK389_05065"
                     /inference="COORDINATES: protein motif:HMM:NF037628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23102.1"
                     /translation="MKPAQRVVLWYIRTKFKLIASISKKKAAEKAFDLFCTPQRRKRK
                     KLPKIFEEAAQLQLQNMRLEPVIWTEGVLEKAHFNLALVDILSQLEPFGREFESPIFE
                     CEAILRQLCFIGDGTHARVLLEIEDMRLKGVWFKLRQTTQSVVSVNVGDTVKVAFSVK
                     ANTFRNERTLDIHVVHMHLYP"
     gene            complement(583..1758)
                     /locus_tag="PK389_05070"
     CDS             complement(583..1758)
                     /locus_tag="PK389_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23103.1"
                     /translation="MTELSNLLVNKNVIYKNQVGYTSLFEKDFMAFPNRINHDVNAHT
                     FELSITKGVGSTKKWNAHQDQSIEQVAYYSPKDQTDPSKVIAKVEFEGWRRYVAERRP
                     IEGGRIIFHVRGLQKQSTLARAIDDQSFREIGWMEPIVAVMEDPILAINFRETAIEDN
                     PDYIDDLFSRIHRVEMLPHEIMIDLKNVIALNSIDRFKKRMIESGFSEYEFEEALKSI
                     RPTALKGVASPFYVAALFCRTKGKFKEARRLLKKIGKNHWQYTEASDLERSLYEQEIR
                     ENKQKYERIIQALKTEVVHLRSRGSIADHTSGTGSSLQLKDTAIAQQDFTIKIQQKAE
                     TLKKTNAEHTQRLTETSPSLAKEIIPYFQGLQGLLSQRHNATQTENTPKKRKRQRVT"
BASE COUNT          549 a          358 c          395 g          650 t
ORIGIN      
        1 acactaacca tgtcaacttt ctgtacacta cttttgcagc atgaaacctg ctcaacgcgt
       61 agtgttatgg tatatcagga caaaattcaa actaattgcg tctatctcca aaaagaaagc
      121 ggcggaaaag gcttttgatc tgttttgcac accgcaacgc cgcaaacgga agaaattacc
      181 aaaaatattt gaagaagcgg cgcaacttca acttcaaaat atgcgattag aacccgttat
      241 atggacagaa ggtgttttag aaaaagcaca ttttaattta gcacttgttg atattctgag
      301 tcaattggaa ccgtttggtc gagaatttga atcgcctatt tttgaatgtg aggccatttt
      361 aaggcagctc tgctttatcg gcgatggtac gcatgcccgt gtattgttgg aaatagaaga
      421 tatgcgattg aaaggggttt ggtttaaatt aagacaaacg actcaatctg tcgtttcggt
      481 aaatgtgggg gatactgtga aagtcgcttt ttcagtcaag gccaatacct ttagaaatga
      541 acggacactc gatattcacg ttgttcatat gcatttatat ccttaagtaa cgcgttgccg
      601 tttgcgtttt ttaggcgtat tttctgtttg cgtggcattg tgccgttgtg agagaagtcc
      661 ctgtagccct tgaaaataag ggattatttc tttagcaagt gacggggatg tttctgttag
      721 ccgttgtgtg tgttctgcat tagttttttt cagggtttct gctttttgtt gtatcttgat
      781 tgtaaagtct tgctgcgcga tagcggtgtc ttttaattga agcgaggatc cagtaccaga
      841 agtgtgatct gcaatagaac cacgtgatct taaatgaaca acttctgttt taagtgcttg
      901 aataatacgc tcatactttt gtttattttc tcgaatttct tgctcataga gtgatctttc
      961 gagatcactc gcttctgtat attgccaatg atttttgccg atcttcttta gcagtcttct
     1021 cgcctcctta aatttacctt tggttctaca aaaaagcgct gctacataaa atggtgaggc
     1081 cacgcctttt aaggctgttg gacggattga ttttaaggct tcttcgaact catactccga
     1141 aaatccggac tcaatcatgc gttttttaaa gcgatcaatg gaatttaagg cgataacatt
     1201 tttcaaatcg atcataattt catgtggaag catttcgact cgatgtatac gcgaaaatag
     1261 atcgtcaata taatcgggat tatcttctat ggcagtttct cgaaagttaa tcgctaaaat
     1321 aggatcttcc atgactgcaa cgatgggctc catccatcca atttctctaa agctttgatc
     1381 atcaatcgct cgggcaagcg tggattgctt ttgtaagcct cgaacatgaa atatgatgcg
     1441 acctccttct atagggcgcc gttctgcgac ataacgcctc cagccttcaa attccacttt
     1501 ggcgataact ttgcttgggt ctgtttgatc tttaggcgaa tagtatgcca cttgttctat
     1561 cgattgatct tgatgcgcat tccatttttt agttgagccg actcctttgg ttatggaaag
     1621 ttcaaatgtg tgtgcattaa catcgtgatt gattctattg ggaaatgcca taaaatcttt
     1681 ttcaaacagc gaagtgtaac caacctggtt tttataaata acatttttat taaccaacaa
     1741 attcgaaagc tctgtcactg acaaatccct gtaataattc acttctggaa gcgagaatga
     1801 acgagatcat aatggaaaat attctgtaag tacatgagaa tttgattgtt gtaaaattag
     1861 aaaaataact gtattaaaaa acagtatttt gatattgaat gaatccgtaa aatgcaatca
     1921 tgttattcaa cgaatttaat ttatcgttag gc
//
LOCUS       DAORVT010000234         1952 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_63858_length_1952_cov_3.132314, whole genome shotgun sequence.
ACCESSION   DAORVT010000234 DAORVT010000000
VERSION     DAORVT010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63858_length_1952_cov_3.132314"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PK389_05075"
     CDS             <1..225
                     /locus_tag="PK389_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23104.1"
                     /translation="IASDASDNPDTPLIAETIRIAIERFATDTQIPVFKNDQFGHGPT
                     NYPIVYNAKSDIIVNEKPGEFSWVMHLESK"
     gene            222..1520
                     /locus_tag="PK389_05080"
     CDS             222..1520
                     /locus_tag="PK389_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY23105.1"
                     /translation="MNKTYTINNISSQIKKNKAIFLSCLGAILEYYDFIIYGMMVIYL
                     SEIFFPASNTDVAYLKTFSILSIGYIARPIGGYLFGLVADIYGRKKTLLMVMLVMAAS
                     TMMIGLLPTYEAIGIFSPILLTLARVLQGLSFGAEMPNMTTIIKENTKTGSSGKYFGI
                     MMSSTAIGTLLASLVVAIITRQFLHDEIIAWVWRLPFLLGGTLALLVFIMRTRIEETP
                     DFLKGQQSAILHRSSKQLTRDLFKSHWKDLLQGMSGTLFFSYLIIFSLYLPIYLKQYF
                     NYTAEAIFSWMTFSILLTVFWSPLFGHLFDRLNREKALKILTLFFLLFLAVSLKLLNL
                     NSQFALAFFLLGYQLFIASYATNNFVVLSTLFPVNMRSTGIGLCYNTAYSIASVTPLI
                     LTPLINKMNYGFIVMLFSIIVVLLTVTGCSFQRNRVPATP"
     gene            complement(1568..>1952)
                     /locus_tag="PK389_05085"
     CDS             complement(1568..>1952)
                     /locus_tag="PK389_05085"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HQY23106.1"
                     /translation="TGTPAARQLRAQFKNSTILANVYYEFLGLNRFKPYLTVGLGPSV
                     NSVQSSITAAPYDAAGSATLRKFGVAWAGGVGLRVAVFSRWHIDVSYRYIRLGSGINI
                     KPTSQFKLLGATSLNAVSLGLMYYF"
BASE COUNT          585 a          428 c          344 g          595 t
ORIGIN      
        1 atagcctcag atgcatcgga taacccagat actcctctta tcgctgagac aattcgcatc
       61 gctatagaac gcttcgccac agacacgcaa attcctgtgt ttaaaaacga tcaatttggt
      121 catggaccta ccaattaccc gattgtttac aatgcaaaat ccgacattat cgtaaatgaa
      181 aaacccggtg aatttagctg ggttatgcat ttggagagca aatgaacaag acctacacaa
      241 tcaacaacat cagcagccaa ataaaaaaaa ataaggctat tttcttaagt tgtcttggcg
      301 caattttaga atactatgac tttattatct atggaatgat ggttatctat ctttcagaaa
      361 tcttttttcc agccagtaac acagatgtgg cttatttaaa aacattttcc attttatcga
      421 ttggatatat tgcccgccct atcggtggat atctatttgg tctagtagca gatatttatg
      481 gcagaaaaaa aacacttctt atggttatgc tggtcatggc tgcgtcgacg atgatgattg
      541 gcttactacc aacctatgaa gccattggga tattctcacc gatattatta acccttgcac
      601 gcgtactgca aggactttct tttggcgcag aaatgcccaa tatgacgacc attatcaaag
      661 aaaacacaaa aacagggtct tcaggaaaat actttggtat tatgatgtct agcaccgcta
      721 tcggcacctt gctcgcctct ttagtagtcg caattatcac acgtcaattt ttacatgatg
      781 aaattattgc ttgggtttgg cgacttcctt ttctattagg cggcacttta gccttacttg
      841 tgtttatcat gagaacgcgc atcgaggaaa ctcctgattt tctaaaggga caacaaagtg
      901 ccattttgca tcgttcttct aaacaactca cccgcgattt atttaaatcg cactggaaag
      961 atttactaca gggcatgtct ggcaccctgt tcttttctta tctgattatt ttcagcctgt
     1021 acttaccgat ttatttaaaa caatatttta attacacggc tgaagccata ttttcttgga
     1081 tgacattcag tatactactc accgtttttt ggtcgcccct atttggacat ttattcgatc
     1141 gcttaaatag agaaaaagcc cttaaaatac taacgctatt ttttctactc tttttagctg
     1201 tatctttaaa attattaaac ctaaattcac aatttgcatt agcgtttttt ctattaggct
     1261 accagctctt tattgcgagt tatgcaacca ataattttgt ggttttatcg acactgtttc
     1321 cggtaaatat gcgctcaaca ggcattgggc tttgttataa tacggcatat tccattgcca
     1381 gcgtaacgcc gttgatatta acacctctga taaataaaat gaactatggc tttatcgtta
     1441 tgcttttttc aatcattgtc gtgctcttaa ctgttacagg atgctcgttt caacggaata
     1501 gagtgccagc cacaccctaa aatatagcac aggcggttat gaaaaaagca ttgtaggcaa
     1561 aacgtactta gaaataatac atcaagccca agctaaccgc attcagactc gtagcaccca
     1621 ataatttaaa ctggctggtc ggcttaatat taataccact gcccaaacga atataacgat
     1681 agctaacatc aatatgccaa cgcgaaaaca cagcaacgcg taaaccaacg cctccagccc
     1741 aagcaacccc gaacttgcgc aaagtcgcag aacccgcggc atcatatggc gcagctgtaa
     1801 tgctggattg cacgctgttg accgacggac caagccctac ggttaaataa ggcttaaatc
     1861 gattgagacc taagaattca taatagacat ttgcaagtat cgtactattt ttaaattgcg
     1921 cccttaattg acgtgccgca ggtgtaccag tc
//
LOCUS       DAORVT010000235         1943 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_64426_length_1943_cov_2.740996, whole genome shotgun sequence.
ACCESSION   DAORVT010000235 DAORVT010000000
VERSION     DAORVT010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64426_length_1943_cov_2.740996"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PK389_05090"
     CDS             complement(<1..371)
                     /locus_tag="PK389_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017443248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HQY23107.1"
                     /translation="MDLDPLLLARIQFAFTISFHIIFPAFTIGLASFLAVIEWRWLKT
                     HDERFRDIYKFWIKIFAVAFGMGVVSGVVLSYQLGTNWSLFSDRVANVIGPLLGFEVL
                     TAFFLEASFLGIMLFGWDRVS"
     gene            475..1785
                     /locus_tag="PK389_05095"
     CDS             475..1785
                     /locus_tag="PK389_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HQY23108.1"
                     /translation="MQNKNTPLAARLRPSTLNDFVGQAHLLAEGKPLWQALQAGQPHS
                     MILWGPPGTGKTTLARLLAKTADTAFISISAVMSGLKDVREAIDKATELAKTQQQKTI
                     LFVDEVHRFNKNQQDVFLPYVENGTIIFIGATTENPSFEINNALLSRCRVYVLKSLTE
                     QDLLEIIDRALTHSTLNTELNLPDNKTISFDLPLRKLLVQSADGDARQALNLLELSLR
                     HAKKQDNEIIIDEEILQAVVSGTLNRFDKGGDHFFDQISALHKSVRGSSPDAALYWFS
                     RMIIGGCDPLYIARRVVRMASEDIGNADPKALQLALNAWEVQERLGSPEGELAIAQAI
                     VYMAVAAKSNAVYMALKTAMHDAKVNGSLDVPIHLRNAPTKLMRSMEYGAAYRYDHDE
                     PNAFSAGQTYFPEKMGERKYYHPVTRGLEIKIKEKLELLKSLVY"
BASE COUNT          634 a          415 c          388 g          506 t
ORIGIN      
        1 tgactaaccc gatcccagcc aaacaacata ataccgagga aagaagcttc taaaaagaag
       61 gcggttaata cttcaaagcc caaaagcgga cctatcacat tagcgacgcg atcggaaaat
      121 aacgaccagt ttgtaccaag ctgatacgac agcacaacac ctgacaccac gcccatacca
      181 aaggcaaccg caaaaatttt gatccaaaat ttataaatat cacgaaaacg ctcatcgtgc
      241 gtttttagcc aacgccattc tataaccgct aaaaaactcg ccaacccaat cgtaaatgca
      301 ggaaagataa tatgaaaact gattgtaaaa gcaaactgaa tccgtgcaag aaggagggga
      361 tctaaatcta tcataaaatt taccaacctt gaacatacct acgagccttg attttaccac
      421 agatagtcta tatcatacag actgataatc ttaatttaat ctattatttt cgttatgcaa
      481 aataagaaca cccctttggc tgcccgttta agacctagca cacttaatga ttttgttggt
      541 caagctcact tattagctga gggcaaacca ctctggcaag cactacaagc agggcaaccc
      601 cattccatga tactttgggg accaccgggt accggaaaaa caacactggc aaggctgttg
      661 gctaaaaccg cagataccgc atttattagc atctctgctg taatgtctgg tcttaaggat
      721 gttcgagaag ccatagacaa agcaacggag ttggctaaaa cacagcaaca aaaaaccatt
      781 ttatttgtag acgaagtcca tcgatttaac aagaatcaac aagatgtttt tctaccctac
      841 gtcgaaaatg ggacaattat ttttattggc gccacaacgg aaaatccttc ttttgaaatt
      901 aataatgcgt tgttgtcacg ctgtcgggtt tacgttttaa aatccttaac agaacaagat
      961 ttgctagaga tcatcgatcg cgcgctgaca cattcaacgc taaatacaga gctgaatcta
     1021 ccagataaca aaacaatttc ttttgatttg cccttgcgaa aattattagt tcaatccgca
     1081 gatggtgatg cacgccaagc tttaaattta ctggagctct cgcttcgcca tgcaaaaaaa
     1141 caagacaatg aaattattat tgatgaagaa atactgcaag cggtggtttc gggaacgctg
     1201 aatcgtttcg acaaaggtgg cgatcatttt tttgatcaga tttctgcact gcataaatcc
     1261 gttcggggtt cttcgcccga tgcagcgctt tattggtttt cacgcatgat tataggtggc
     1321 tgtgatccac tctatatcgc gagaagagtg gttcgaatgg ccagtgaaga tattggtaat
     1381 gcggacccca aagccttaca acttgcgctt aatgcgtggg aagtacaaga gcgactgggt
     1441 agcccagaag gggagttggc tatcgcacaa gcgattgttt atatggcagt tgcagcgaag
     1501 agtaatgccg tttacatggc gctaaaaacg gctatgcacg atgcaaaagt gaatggttct
     1561 ctcgatgtgc ctattcactt acgcaatgcg ccaaccaaac tgatgcgctc gatggaatat
     1621 ggcgcagcgt atcgatatga tcatgatgag cctaatgctt tttcggctgg acaaacctat
     1681 tttccagaaa aaatgggcga gcgaaaatat tatcatcctg taaccagagg actcgaaatt
     1741 aaaataaaag aaaaattgga attgttgaaa agtttggtgt attaaaatac ccatcatttt
     1801 tcccaaacgg gaaaaatggt ggctgcaaac acagtctcac atcgaaaaat ttctgagata
     1861 tagataaact tcagcgcgtt atactaagcg ccatatgagc ttcgtcataa aatttgtcat
     1921 taattctcat ggctttcaat tct
//
LOCUS       DAORVT010000236         1938 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_64731_length_1938_cov_2.823686, whole genome shotgun sequence.
ACCESSION   DAORVT010000236 DAORVT010000000
VERSION     DAORVT010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64731_length_1938_cov_2.823686"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="PK389_05100"
     CDS             complement(<1..394)
                     /locus_tag="PK389_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006984218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HQY23109.1"
                     /translation="MNKQFKARSPAKLILSGEHAVVYGKPAIAMAIDRYAESTINANF
                     SSTILFNCLNFKSAKSFTLQALEGIKHRLQDKYHAFLEGRCSIRDVLKTPFELLQYTV
                     THLLETLNVTLSDGLEIRATSEIPIGCGM"
     gene            complement(400..1575)
                     /locus_tag="PK389_05105"
     CDS             complement(400..1575)
                     /locus_tag="PK389_05105"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020285085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HQY23110.1"
                     /translation="MIKVGIDAIAFYTPHYYLDLRVLAKARGVDVNKYHTGLGQEKMA
                     VIPPDEDVVTMAADAASRVLQDVNLNDIDLLLFATESAIDQSKAAGIFVHRLLNLPAR
                     CRVVELKQACYSATAGLQMALPMLREDPNKKILLIASDVARYGLESTGESSQGGGAVA
                     MVLSQNPRILAIEPESGFHTEDVMDFWRPNYREEAIVDGKYSCDLYLKLVQKTWEQYT
                     DLSGRTFSDHARFCYHVPVPRLVEKAHQCLAKFNGLPKLTSLELSAQLGSSLQYGRVI
                     GNCYTAALYLSLISLLENTPEDLAHQRIGFYSYGSGCIAEFFSGVVQKDYQDRLKKND
                     HQKMLENREALSEEEYTAFYNFRYPVDGGALIITNHNKRQYRLAAIEQHKRIYEKLD"
     gene            complement(1572..>1938)
                     /locus_tag="PK389_05110"
     CDS             complement(1572..>1938)
                     /locus_tag="PK389_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019617960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HQY23111.1"
                     /translation="LLALYLATGQDAANIIEGSQGMVHAEVRNQDLYFSVTLPNVIVG
                     TVGNGKHLPFVKENLAGLGCLEPRTPGQNSRRLAAIVGATVLCGELSLLAAQTNPGEL
                     MESHLRLERLESKKVEQKI"
BASE COUNT          581 a          468 c          338 g          551 t
ORIGIN      
        1 ccataccaca accaatcgga atttcggatg tggcgcgaat ttccaaccca tcagacaagg
       61 taacgtttaa agtttctaac aaatgtgtaa cagtatactg caataactca aaaggggtct
      121 ttaacacatc tcgaatacta caacgcccct ccaaaaaggc atgatattta tcttgcaatc
      181 tgtgttttat cccttctaat gcctgtaacg taaatgattt tgcagactta aaatttaaac
      241 aattaaacaa aatagtggaa gaaaaattcg catttattgt gctttctgca taccgatcga
      301 ttgccatcgc aatggcaggt tttccataaa caaccgcatg ctcacccgac aaaatcaact
      361 tggcaggcga tcgcgccttg aattgcttat tcattaatct taatccaact tctcgtaaat
      421 acgcttatgt tgctcgatag ctgcgagtcg atattgacgc ttattatgat tagttataat
      481 aagagccccc ccatcgacag gatagcgaaa attgtaaaat gcggtatatt cttcttctga
      541 cagcgcctct cgattttcta acattttttg gtgatcattt tttttaagtc tgtcttgata
      601 atctttttga acgaccccac taaagaactc tgcaatgcac ccagagccat aactataaaa
      661 gccaatacgc tgatgcgcta agtcttcggg cgtattttct aacaatgaaa tcaagcttaa
      721 ataaagcgct gctgtataac agttgccaat aacacgccca tattgtaacg aagaacccag
      781 ttgtgcactt aattccagag aggttagttt gggtaatccg ttgaattttg ccaaacattg
      841 atgcgctttt tccactaaac gtggaacggg cacatggtaa caaaaacgcg catgatccga
      901 aaatgttcga cctgacaaat ctgtatattg ttcccaggtt ttttgaacca attttagata
      961 aagatcacac gaatatttac catcaacaat cgcctcttca cgatagtttg ggcgccaaaa
     1021 atccatcaca tcttctgtat gaaatccgct ttcaggctca atcgctaaaa ttctggggtt
     1081 ctgtgacaac accatagcaa cggcaccgcc accttgcgac gactcccctg tagactctaa
     1141 accataacgc gcaacatcag acgcaatcaa caatattttt ttattgggat cttcgcgcaa
     1201 catcggtaac gccatttgta accctgccgt agcgctatag caggcttgct ttaattcaac
     1261 caccctacaa cgcgctggca aattcaataa tcgatgtaca aaaatacccg ccgctttaga
     1321 ttgatcaata gcactttcgg ttgcaaacaa caataaatcg atatcattaa gattaacgtc
     1381 ttgtaacacg cgagaagcag catctgccgc catcgttacg acatcttcat cgggtggaat
     1441 aaccgccatt ttttcttgac ccaagcctgt atggtattta ttcacatcca caccacgcgc
     1501 ttttgccaat accctgagat ccaaataata atggggagta taaaaggcga ttgcatcaat
     1561 tcccactttt atcatatttt ttgttcgacc tttttggatt ctaaacgttc caatcgaaga
     1621 tgactttcca tgagttctcc tggatttgtt tgagccgcta aaagagaaag ctccccacac
     1681 aatactgttg cgcctacaat ggctgctaaa cgtctactat tttgcccggg tgtgcgaggc
     1741 tctaagcaac ctaaaccagc caagttttcc ttcacaaaag gcaaatgttt tccgttgcct
     1801 accgtaccca caatgacatt gggcaaggtg acagaaaaat acagatcttg atttcgtact
     1861 tccgcatgaa ccatcccttg cgatccctca ataatattgg cagcatcctg acctgtcgct
     1921 aaataaagtg cgagcaac
//
LOCUS       DAORVT010000237         1927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_65416_length_1927_cov_3.172009, whole genome shotgun sequence.
ACCESSION   DAORVT010000237 DAORVT010000000
VERSION     DAORVT010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65416_length_1927_cov_3.172009"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PK389_05115"
     CDS             complement(<1..232)
                     /locus_tag="PK389_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013942880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HQY23112.1"
                     /translation="MANIKLKSAFFGRKQELRKLNLLLKKNSASLVVVKGRRRIGKSR
                     LIQEFGKDLKMISIAGLTPTDTTTLQDELNAFG"
     gene            complement(458..754)
                     /locus_tag="PK389_05120"
     CDS             complement(458..754)
                     /locus_tag="PK389_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016861616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23113.1"
                     /translation="MSFKLLFTDQASTDFDSLEKDKSLSKCFKAVRKALGYLETNPRH
                     PSLNTHKYDSISGPNGESVFEAYAENKTPAAYRIFWYYGSGKKQIIILAITPHP"
     gene            complement(754..1020)
                     /locus_tag="PK389_05125"
     CDS             complement(754..1020)
                     /locus_tag="PK389_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012151862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23114.1"
                     /translation="MLPKMVRPDAKGRITLGHLADGVSGYMITETKDHRIILKPYVEI
                     LANEKWLLDNKRALKQVSQGLKDAAAGRISEKGSFAKFVDDDIE"
     gene            1235..1331
                     /gene="ffs"
                     /locus_tag="PK389_05130"
     ncRNA           1235..1331
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK389_05130"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1378..>1927
                     /gene="dnaX"
                     /locus_tag="PK389_05135"
     CDS             1378..>1927
                     /gene="dnaX"
                     /locus_tag="PK389_05135"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HQY23115.1"
                     /translation="MSQLALARRYRPHTFQEMVGQDLTVRALCNALDSKRLHHAYLFT
                     GTRGVGKTSVARILAKCLNCEQGISSTPCGTCFACEAIDQGRFVDLIEVDAASRTKVE
                     DTRELLDNVQYLPTRGRFKIYLIDEVHMLSGHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPATILSRCLQFQLWRFPVNKI"
BASE COUNT          536 a          390 c          385 g          616 t
ORIGIN      
        1 aaccaaaggc attcagttca tcttgcaggg tggttgtatc cgtgggggtt agccctgcga
       61 tggaaatcat cttcagatcc ttcccaaact cttggataag gcggcttttg ccgatacggc
      121 gtcttccttt aacaacaacg agactagcag aatttttttt caataataaa ttgagtttcc
      181 tcaattcttg ttttcgtccg aaaaaggcgc tttttagttt aatattagcc attatttacg
      241 tttccctttt gtttttatac gcatcaccaa atctgcaaat gtcgatttgg tgatgaaaga
      301 ctcagtctaa cctaatatca ccaaatctgc aaatgtcgat ttggtgatgc tcgatatagg
      361 gccgtcacgc taacctatta tcaaatattt gtattctaat ttgataacgc attgatgctc
      421 tggtaagtaa agctggatcc acgtgccaat tcaaacttca aggatgtggt gttattgcta
      481 atataatgat ttgtttctta ccagatccat aataccaaaa aatgcgatag gctgcgggcg
      541 tcttgttttc agcataagct tcaaaaactg attcaccatt tggaccgctg atagaatcat
      601 atttgtgcgt atttaagctt ggatggcgcg ggttggtttc taagtatcct agtgcttttc
      661 tcactgcttt aaaacatttt gaaagacttt tgtctttttc taaactgtca aaatcagtgc
      721 tagcttgatc tgtaaataat aatttaaatg acattattca atatcatcat caacaaattt
      781 agcaaagctg cctttctcag aaatgcgacc tgctgccgca tcttttaaac cttgggaaac
      841 ttgctttaat gcgcgtttgt tatctaaaag ccatttttca ttggctaaaa tttctacata
      901 aggttttaaa atgattcgat gatctttggt ttcagtaatc atataaccgc tcacaccatc
      961 ggctaagtgc cctagtgtaa tcctaccttt agcatctggc ctcaccattt ttggtaacat
     1021 aatattaaac cttacaaaat atgttgttga cagtttttag attagtataa agtgggaaag
     1081 aatacaagtg gggaagtgta aaggtggagg ttttgcgatg agcaattctg ataagctgac
     1141 tttaaaacca gcatgatttc aataataatt taagcctctt ctactattga agctagctcc
     1201 tctcccctgt aaactatctt ttcaatggta cccataccgg ttccctcgcg acgatatact
     1261 gtgaaccccg ccagatccgg aaggaagcaa cggtagcagt ggattcgggc gccgaggtgt
     1321 ggctgttatg ggtgccaccc aatctccaaa ataacttggt atgaaattgt aaacgacatg
     1381 agtcaactcg cgcttgccag acgatataga ccgcacacct ttcaagaaat ggtggggcag
     1441 gaccttactg tgcgtgcgct ctgtaatgcg ctcgattcta aacgtctaca tcatgcctat
     1501 ctctttaccg gtacgcgtgg cgtaggtaaa acgagtgttg cgcggatttt ggccaaatgt
     1561 cttaattgtg aacaggggat ttcatccacg ccgtgtggaa cttgctttgc atgcgaagcc
     1621 attgatcaag gtcgctttgt tgatttaatc gaagtggatg cggcttcgcg taccaaggtc
     1681 gaagatactc gagaattatt agataatgtt caatatttac cgacacgtgg ccgctttaaa
     1741 atctatttga ttgatgaagt gcatatgtta tcggggcaca gttttaatgc tttattaaaa
     1801 accctagaag aaccacccga gcatgttaaa tttttactcg ctacgaccga tcctcaaaaa
     1861 ttaccagcaa ctattttgtc tagatgttta cagtttcagt tatggcgatt ccctgttaac
     1921 aagatag
//
LOCUS       DAORVT010000238         1917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_65998_length_1917_cov_3.437701, whole genome shotgun sequence.
ACCESSION   DAORVT010000238 DAORVT010000000
VERSION     DAORVT010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65998_length_1917_cov_3.437701"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..820
                     /gene="kdsA"
                     /locus_tag="PK389_05140"
     CDS             20..820
                     /gene="kdsA"
                     /locus_tag="PK389_05140"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HQY23116.1"
                     /translation="MPIRAKPFFLIAGPCVIESEALALDTAGFLKEMADRLGIHFIYK
                     SSFDKANRTSDESFRGLGIEEGLRILELVKKSIGVAVLTDVHEDTPLSEVSSVVDVLQ
                     TPAFLCRQTNFIQNVARCGLPVNIKKGQFLAPWDMKNVVSKAQSVGNNQIMVCERGVS
                     FGYNNLVSDMRSLAILRETGCPVVFDVTHSIQLPGGQGKASGGQREFVPVLARAAIAV
                     GVAGIFMETHPDPDKALSDGPNSWPLDKMEQLLKTLMQIDKVVKDGRD"
     gene            807..>1917
                     /gene="eno"
                     /locus_tag="PK389_05145"
     CDS             807..>1917
                     /gene="eno"
                     /locus_tag="PK389_05145"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HQY23117.1"
                     /translation="MAEIKDLKAREILDSRGTPTIEVDVVLESGIIGRASAPSGASTG
                     MHEAVELRDKEARYSGKGVLKAIHHVHTEIKTALLNHDVRHQKGIDDILIRLDDTPNK
                     SRLGANALMAVSVAAARAASRAVKLPFYQYLNQNQLEIKHSILPVPLMNVINGGAHAD
                     NNIDIQEYMILPVGAPSFKEAIRYGVEIFQALKSLLKQKGLSTSVGDEGGFAPHLSSN
                     EAAMELILEAIQIAGFTPGKEIFLALDLASSEFYSEGKYHLAGDNKNFSSEEWVSHLV
                     KWVNQYPILSLEDGMAEEDWQGWKCLTEALGKRIQLVGDDLFVTNTKMLIRGIQEQIA
                     NAILIKPNQIGTLTETFHAIQMAQEAHFSTIISHRS"
BASE COUNT          574 a          327 c          464 g          552 t
ORIGIN      
        1 gaaattttaa ttttaggtaa tcccaattcg ggcaaaaccc ttctttttaa tcgcaggtcc
       61 ttgtgtgata gaaagcgaag cgttagcgct agacacagcg ggttttttaa aagaaatggc
      121 agatcgctta ggcattcatt ttatctataa atcttcattt gacaaagcca atcgtacttc
      181 agatgaatct tttcggggat tgggtataga agaaggttta cgcattttag aattggttaa
      241 aaaaagcatt ggtgtagcag tcttaactga tgtgcatgaa gatacgcctt tatccgaagt
      301 ttcatctgtt gtggatgttt tgcaaacacc ggcgttttta tgtcgccaaa caaattttat
      361 tcagaatgtc gcacgatgcg gtttgcccgt gaatattaaa aagggtcaat ttttggcgcc
      421 ttgggatatg aaaaatgtgg tctctaaagc acagagtgtt gggaataatc agattatggt
      481 gtgcgagcgc ggggttagct ttggatacaa taatttagtg tcggatatgc gttcattggc
      541 tattctaaga gaaacaggtt gtcctgttgt gtttgatgtg acgcattcta ttcagttgcc
      601 gggtgggcaa ggcaaagctt cgggtggaca gcgagagttt gtaccggtct tggctcgcgc
      661 agcgattgcc gttggggttg ctgggatttt tatggaaaca caccctgatc cagataaggc
      721 tctcagtgac ggtccaaatt catggccatt ggataaaatg gagcaattac tcaagacctt
      781 aatgcaaata gacaaggtgg tgaaagatgg cagagattaa agatcttaaa gcaagagaga
      841 ttttagactc cagaggcacg cccacaatag aggtcgatgt cgtattagaa tccggcatta
      901 tagggcgtgc cagtgcgcct tcgggtgctt ctacaggcat gcacgaagcg gtagaattaa
      961 gagacaaaga agcgcgatat agtggaaaag gcgtgttaaa agcgatacat catgttcaca
     1021 ccgaaattaa aacggcatta ttaaatcatg acgttcgtca tcaaaaaggg atagatgata
     1081 ttttaatccg tttggatgac acaccgaaca agagtcgctt gggcgcaaat gcactgatgg
     1141 ccgtttctgt tgcagcagcg cgtgcggctt cccgtgctgt taaattgcct ttttatcagt
     1201 atttaaacca aaatcagctc gaaatcaaac attcaatatt accggttcct ttaatgaatg
     1261 tgattaatgg tggggcacat gcggataata atattgatat acaagaatat atgattctgc
     1321 ctgtgggtgc gccgagtttt aaagaagcca ttcgttatgg ggttgaaata tttcaagcat
     1381 taaaaagttt gttaaaacaa aaaggcttaa gcacaagtgt tggagatgaa ggcggttttg
     1441 cgccacattt atcttctaac gaagcagcca tggaattgat attagaagcg atacaaatag
     1501 ccggttttac ccctggaaaa gagatttttc tggctcttga tttggccagt agtgaatttt
     1561 atagcgaggg caaatatcat ttagccggag acaataaaaa ttttagttct gaagaatggg
     1621 tttctcattt agtgaaatgg gtcaatcaat atcctatttt atctttggaa gatgggatgg
     1681 cagaagaaga ttggcaaggt tggaaatgct taacagaagc tttgggtaaa cgcattcaat
     1741 tagtcggaga tgatcttttt gtgacgaata cgaaaatgct gattcgggga atccaagaac
     1801 aaattgccaa tgccattctg attaagccaa accaaattgg cacattaacc gaaacgttcc
     1861 atgcgattca aatggcacaa gaggcgcatt tttcaacgat tatttcacat cgttcag
//
LOCUS       DAORVT010000239         1913 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_66281_length_1913_cov_2.068353, whole genome shotgun sequence.
ACCESSION   DAORVT010000239 DAORVT010000000
VERSION     DAORVT010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66281_length_1913_cov_2.068353"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PK389_05150"
     CDS             <1..327
                     /locus_tag="PK389_05150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY23118.1"
                     /translation="NIVQVLSEGSLFVLSSRYEGFPNSLCEAMGVGLPVIAFDCGSGS
                     ADIIRHNIDGLLVPAQNVNALTNAMLQLIENIELRKQYGKRAKEIIERFSLKKILKKW
                     DELTHC"
     gene            complement(344..1519)
                     /locus_tag="PK389_05155"
     CDS             complement(344..1519)
                     /locus_tag="PK389_05155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY23119.1"
                     /translation="MKKTIIIVVNSLDVGGTERHLISVLPKLVSNGWPVRVITTFKKG
                     VLASEFEKRGIPVCCVLDGKYLTWIQKLPQLFSHLIRMILGVFALKSYVKKRIQDHEN
                     TILHFYLPEAYIVGMLAAGLAQFKGFKIMSRRSLNCYQQKSVFIGWLEKKLHSRTSVI
                     TGNCQALVSQLQIEENVPLEHLRLIYNGIDTAAFIPTESRNAVRSTLGIQQDALVIVK
                     VANLIPYKGHVDLLVALSKIKDKLPECWYLICLGRDDGIGSSLRQQAENLGLSEHILW
                     LGTRSDISNVLFSADIGVLCPFKNEGFSNAILEGMTAGLPMVVTDIGGNKEAVINGKT
                     GYVVEPRHTDSLAAAILDLANNLSKAKQFGEAGQKRVKESFSLSACVDAYEKLYNSL"
     gene            complement(1516..>1913)
                     /locus_tag="PK389_05160"
     CDS             complement(1516..>1913)
                     /locus_tag="PK389_05160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY23120.1"
                     /translation="LGLHDKVSLPGAIQNIQEALIKSDLFIFPSQYEGFPNALCEAMA
                     VGLPVIASDCSGNIDIVRDGIDGRLFPVGDMQTLTKITLELLEDGLQRQHLAEHAKEI
                     CDRFEPDRIFKLWDQVITDAACQHSETLK"
BASE COUNT          603 a          428 c          335 g          547 t
ORIGIN      
        1 aacatagtcc aagttttatc agaaggttcg ctatttgtac tctcttctcg gtacgaagga
       61 tttccaaata gcttatgcga ggcgatggga gtcgggctgc ctgttattgc ttttgattgc
      121 gggagtggat cagcagacat tatccgacat aacattgacg gtcttttagt acctgctcaa
      181 aatgtgaatg ctttaaccaa cgcaatgttg caattgatag aaaacatcga attacgtaaa
      241 caatatggca aacgtgcaaa agaaattatc gagcggtttt cgttgaaaaa gattttaaaa
      301 aaatgggatg agctcacaca ttgttaatct cgtgtaaact tatttacaga ctgttataaa
      361 gcttctcata agcatcgaca caagcgctca atgaaaaact ttctttaaca cgcttctgcc
      421 cagcctcacc aaactgcttc gctttgctga gattattggc taaatctaaa atggccgctg
      481 ctaagctgtc cgtatggcgt ggttctacaa cataacctgt tttgccattg ataacagctt
      541 ccttattccc gccaatatcc gttacgacca tgggtagacc cgcagtcatc ccctctaata
      601 tcgcatttga aaacccctcg tttttaaagg gacacaaaac accgatgtcg gcactaaaca
      661 acacatttga aatatccgat cgagtaccta gccacaaaat gtgttcactt aagccaagat
      721 tctctgcctg ctgtcgtaaa gaactgccaa tcccatcgtc acgccccaga caaattaaat
      781 accagcattc tggaagctta tccttaattt tactcaaagc gaccaataga tcgacatgtc
      841 ctttgtatgg aataagatta gcaactttca caataaccaa agcgtcttgt tgaatgccaa
      901 gtgtacttct aaccgcattg cgagattccg taggaataaa agcagctgtg tctatgccat
      961 tgtaaatcag tcgtaaatgt tctaagggta cgttctcttc tatttgtaac tgcgaaacaa
     1021 gcgcctgaca atttcccgta atcactgaag ttctagaatg taactttttt tctaaccaac
     1081 ctataaaaac actcttctgt tgatagcaat tcaaggagcg tcgactcatg attttaaatc
     1141 ccttaaattg tgccaaaccc gccgctaaca tgccaacaat ataagcttct ggtaaataaa
     1201 aatgtagaat tgtgttttca tgatcttgaa ttcgcttttt tacgtacgac tttaaagcaa
     1261 aaacacctaa aatcatacga ataagatgac tgaataattg aggtaatttt tgaatccagg
     1321 ttaaatactt cccatctaaa acacaacaaa caggaatccc tcttttctca aactctgagg
     1381 ccaaaacacc cttcttaaat gttgtaataa cgcgcacagg ccatccatta gaaacaagct
     1441 tgggtaaaac agaaattaaa tgccgctccg ttccgccgac atccaggctg ttaacaacaa
     1501 taatgatagt ttttttcatt ttaaagtttc actatgctga caagcagcat cagtaataac
     1561 ttggtcccat aatttaaaaa tacgatcagg ctcaaatcga tcgcaaatct ctttggcatg
     1621 ttcagccaaa tgttgtcgct gtaaaccatc ttctaacagc tctagtgtaa tcttagttaa
     1681 agtttgcatg tcacctactg gaaaaagtct tccatcgatt ccatctcgaa caatgtctat
     1741 atttccggaa caatcagatg cgatcacggg caaacccacc gccatcgcct cacacaaggc
     1801 gttggggaaa ccttcatatt gagaaggaaa gataaaaaga tcggatttaa taagggcttc
     1861 ttgaatattt tggatagcgc ctggcaacga tactttatcg tgcaagccta acg
//
LOCUS       DAORVT010000240         1896 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_67363_length_1896_cov_2.844106, whole genome shotgun sequence.
ACCESSION   DAORVT010000240 DAORVT010000000
VERSION     DAORVT010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67363_length_1896_cov_2.844106"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PK389_05165"
     CDS             <1..429
                     /locus_tag="PK389_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23121.1"
                     /translation="VLNFNNKILSKSTINHEEFYGQVYASVRLQNGCASLGEDIIGEQ
                     ANKFCSAISSSASKTLKNAASAKDHTASQFLSQRDVKAYLIHLEELLLRGRTARHGQS
                     STAEKTKRNNYGAELLFFQGLKARRDDTEEIKDRPSTKSR"
     gene            560..>1896
                     /locus_tag="PK389_05170"
     CDS             560..>1896
                     /locus_tag="PK389_05170"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HQY23122.1"
                     /translation="MTSTLFGPYALLLLVLGLILVFIYIQDSRQKEHAILRNFPIIGH
                     FRYILEGLGVYLRQFWFAQDREELPFNRATRSWVYRAAKNEDMTVGFGSTRDLRPVGT
                     VLFVDAPFPVLGEDASEIQPVTIGPYCRIPYVAKSLFNISAMSYGSISKNAVLALAEG
                     AKIGGCWLNTGEGGVSRYHLESGCDLVAQIGTAKYGFCDANGKLSDEALRGVALYPQI
                     KMFELKLSQGAKPGKGGILPAVKVTPEIAEIRFIKAHQDSISPNRHPEISNVQELMDF
                     IEHIRTLTGKPVGFKIVIGSYEWLDDLCIEILKRGIENAPDFITLDGAEGGTGAAPAS
                     LIDYMGLPIAESLPVLIDKLVEYGLRDRIKVIAGGKLITPAEVAWALCMGADFVASAR
                     GFAFALGCIQALKCHTNTCPTGITTHQPHLVQGLDPATKAVRVANYLKRILYET"
BASE COUNT          578 a          358 c          417 g          543 t
ORIGIN      
        1 gttttaaatt ttaataacaa aattcttagt aaatctacta taaatcacga agaattctat
       61 ggccaagtat acgcaagcgt tagattgcaa aacggctgtg cttctttggg agaagatatt
      121 attggtgagc aagcaaataa attttgttca gcaatctcga gctcagcatc caagactttg
      181 aaaaacgctg ccagcgctaa agatcacact gcatcacaat ttttatcaca acgcgatgta
      241 aaagcatacc ttatccatct tgaggaactt ctgcttagag ggagaacggc aagacatggg
      301 caaagctcaa cagcagaaaa aactaaacgc aacaactatg gtgccgaact attattcttc
      361 caaggtctca aagccagaag agatgatact gaagaaatta aggacagacc tagcacaaag
      421 tctcgttaat ttgagttaaa aaacctaaaa attttgggca ttaaacagag cctctcgttt
      481 attccaacat ctaaaaatat agactatcct atatttaagg tatttgatat aaaaatacct
      541 gtacagggag gtataacgga tgacatcgac gctatttggg ccttacgcac tattattgct
      601 tgtgttgggc ttaatattag tctttatcta tattcaggat tctcgacaaa aagagcatgc
      661 cattttacgt aattttccca ttattgggca tttcagatac attttagaag gtctaggagt
      721 ctatttacgt caattttggt ttgcgcaaga tagagaagag cttcccttca accgtgcaac
      781 acgctcctgg gtctatagag ctgctaaaaa tgaagatatg accgtgggat ttggttccac
      841 gcgcgattta cgcccggtag gcaccgtgtt gtttgtggat gcgccatttc ctgtcttagg
      901 agaagacgcc agtgaaatac agcccgttac cataggccca tactgcagaa tcccttatgt
      961 tgccaaatcg ttgtttaata tttctgctat gagttatggt tcgatttcta aaaatgcggt
     1021 gctcgcctta gccgaaggcg ccaaaatagg tggatgttgg ttaaacacgg gggagggcgg
     1081 ggtttcgcgt taccatcttg aatcgggctg cgatcttgtc gcacaaattg gaacagcgaa
     1141 atacggcttt tgtgatgcaa atggaaaact gagtgatgaa gccctaagag gtgttgctct
     1201 ttatcctcaa ataaaaatgt ttgaattaaa gttgagtcaa ggtgcaaaac ccggtaaagg
     1261 aggtatttta ccagctgtta aagtaacacc tgaaattgcc gagattcgtt ttattaaagc
     1321 gcaccaagat tctatcagcc ccaatcgaca ccccgaaatt agtaatgttc aagagttgat
     1381 ggattttata gaacatattc gaacactgac aggaaaaccc gttggattta aaattgtgat
     1441 tggtagctac gagtggttag atgatctctg tattgaaatt ttaaaaagag gtatcgaaaa
     1501 cgcacccgat tttattacat tagatggcgc agaaggtgga acaggcgctg caccggcaag
     1561 tttaattgat tacatgggac tacccattgc tgaaagttta ccggttttga ttgataaatt
     1621 ggttgaatat ggattgcgcg atcgcattaa agtgattgct ggcggaaaat tgattacgcc
     1681 tgccgaagtt gcctgggcat tgtgcatggg tgctgatttt gtcgcttctg cacgtggctt
     1741 tgcttttgct ttaggttgta ttcaagcatt gaaatgccat accaatacgt gtccaaccgg
     1801 tattacaacg catcaaccgc atttagtaca gggattagat cccgccacaa aagcggtgag
     1861 agttgccaat tatctaaaaa gaattttgta cgaaac
//
LOCUS       DAORVT010000241         1895 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_67387_length_1895_cov_5.703804, whole genome shotgun sequence.
ACCESSION   DAORVT010000241 DAORVT010000000
VERSION     DAORVT010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67387_length_1895_cov_5.703804"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /gene="tnpB"
                     /locus_tag="PK389_05175"
     CDS             <1..344
                     /gene="tnpB"
                     /locus_tag="PK389_05175"
                     /inference="COORDINATES: protein motif:HMM:NF017528.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HQY23123.1"
                     /translation="PDVPIYIYNGVTDMRKSINGLVLLVVDILKKDPQSKALYLFYNK
                     NMDKVKSIFWDQNGFILLYKPLEKGRFKFPKDIGGLEYEIDSDLLQWLLKGFDFYALK
                     HHPDLKKSQYF"
     gene            367..>1895
                     /locus_tag="PK389_05180"
     CDS             367..>1895
                     /locus_tag="PK389_05180"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HQY23124.1"
                     /translation="MESKDGLSKISTEILQARLEELRAENARLLLKQDQLILENFEKD
                     KLLVEALAEKNKLLEETERLRHQLNQLLQDKFGTKSEKSKNLPNETFDEATEPDNTPD
                     IEITEQEIFVAAHQRKQGKPGRKPLPAHLPRVRQIYDLEASEKICSCGCQLSKIGEEI
                     SEQLDIIPATVQVIQHVKYKYACQACEDTIKTAKGQKLPIPKSIATAGLLAHVSVSKF
                     KDHLPLYRQENIFRRMGVDIARNTLSLWMIKTAQILTGLYKLAQDIIISDDVAYSDET
                     RVQVLKELNRKAEDKAYMWCFIGGSPEKRCVIYHYNISRAHTVIEEILEGFSGYLHCD
                     GFGGYDAYASDHDVKLIGCWMHCRRKFFEVARSTKSKGLAHAAVQIIKALYDIETEMR
                     DQGLSVQQRYDYRQQYSKPLLEQFKTFLDENRTKVLSKSPLGEAFTYAVNQWPKLIRY
                     IEDGRLEIDNGLSERKIKPFVIGRKNWIFCNSIAGAKAAEVLFSIIETCELHKIEPYA
                     YL"
BASE COUNT          631 a          349 c          390 g          525 t
ORIGIN      
        1 cacccgatgt ccccatctat atttataacg gcgttaccga catgcgtaag tcgatcaacg
       61 gtcttgtatt actggtcgtt gacatattga aaaaagatcc acagtcaaaa gcactatatc
      121 tattttacaa caagaacatg gataaggtta aatccatctt ctgggatcag aatggcttca
      181 ttttattgta taaaccgttg gagaaaggga gatttaaatt cccaaaagac attggcgggt
      241 tagaatatga aattgatagc gacttgttgc agtggctact caaagggttt gatttttatg
      301 cacttaaaca ccatcccgat ttaaaaaaat ctcaatattt ttaatcaaat gtgttatgat
      361 aaatgcatgg aaagcaagga tggtttatca aaaatttcta cagaaatatt acaggcacgt
      421 cttgaagagc tgagagcaga aaacgctcgt ttattgttaa aacaagatca attgatttta
      481 gagaattttg aaaaagataa actgcttgtt gaagccttgg cagagaaaaa taagcttctt
      541 gaagaaaccg agcgattacg gcatcaatta aatcaattgt tgcaggataa atttggcacc
      601 aaatctgaga aaagtaaaaa tcttcctaac gagactttcg acgaagctac agaacccgat
      661 aatactccgg atatcgaaat aactgaacaa gagatcttcg ttgcagccca tcaacgcaaa
      721 caggggaagc ctgggcgtaa accattacct gcacatttac cccgagtgcg acagatttac
      781 gatctggaag catcggaaaa aatttgctct tgtggttgcc aattgagcaa gattggggaa
      841 gaaatcagtg agcaattgga tatcatccct gcaactgtac aagttatcca gcatgtaaaa
      901 tataaatatg cctgtcaagc atgtgaagac accattaaaa ctgccaaagg tcagaaattg
      961 cctatcccaa aaagtattgc aactgctggg cttttagcac acgtcagtgt ttccaagttt
     1021 aaagaccatt tacccctata tcgacaagag aatatttttc gacgaatggg tgttgatatt
     1081 gcccgtaata ctttatcgct atggatgatt aagaccgctc agatattaac cggcctttat
     1141 aaattggctc aggatattat tataagcgat gatgtggctt attccgacga aacccgagtc
     1201 caggtgttaa aggaactcaa tagaaaagca gaggataaag cttacatgtg gtgctttatc
     1261 ggaggctctc cagagaaacg ctgtgtgata tatcattaca atattagcag agcacatacc
     1321 gtcatcgaag aaattctgga agggttttct ggttatttac attgtgatgg ctttggtggg
     1381 tatgatgcct atgcttctga tcatgatgta aaattgatag gctgttggat gcactgccgt
     1441 cgaaaattct ttgaagtagc acgcagtaca aaatccaaag gattagcaca tgcggccgtt
     1501 caaataatta aagcgctata cgatattgaa acagaaatgc gagatcaagg attgtctgtt
     1561 cagcagcgct atgattaccg acaacagtat agcaaaccat tgctcgaaca atttaaaact
     1621 ttcctagatg aaaaccgtac aaaggtttta tccaaatcgc cactgggaga ggcatttacc
     1681 tacgctgtta accagtggcc gaagctaatt cgttatattg aagacggtcg acttgaaata
     1741 gataatggac tctcggagag aaaaataaaa ccttttgtta taggccgtaa aaattggata
     1801 ttttgcaatt ctattgctgg cgccaaagca gcagaagtct tgttttctat tatcgagacc
     1861 tgtgaactac acaaaattga accctatgcg tatct
//
LOCUS       DAORVT010000242         1890 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_67739_length_1890_cov_2.829973, whole genome shotgun sequence.
ACCESSION   DAORVT010000242 DAORVT010000000
VERSION     DAORVT010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67739_length_1890_cov_2.829973"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            305..1591
                     /gene="hemL"
                     /locus_tag="PK389_05185"
     CDS             305..1591
                     /gene="hemL"
                     /locus_tag="PK389_05185"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HQY23125.1"
                     /translation="MDYSTKLYARAQTVIPGGVNSPVRAFKGVGGTPVFFKSGNGAYL
                     FDVEGKKYIDYVGSWGPLIHGHAHPQILKALEAALPQGLGFGAPTEIEVEMAELITEL
                     MPSIEQVRMCNSGTEATQSAIRLARGFTGRNKILKFQGGYHGHTDSLLIKGGSGLLTL
                     GIPDSLGVPEAFTNETLIAPFNNLAAVKTIFEKFGDEIAAIIVEPIACNMNCIPPLPD
                     FLTGLKALCHQYKSLLIFDEVITGFRVALGGAQALYQITPDLTCLGKIIGGGLPVGAF
                     GGRRDIMAYLAPQGPVYQAGTLSGNPIALTAGFTTLQLLREPGVYESLNKATTYLMNG
                     LKNIAEQFNLPLQTHQVGSLFGLFFTTEKTITHFEHVEQCNIEQFKKFFHGMLQKGVY
                     LAPSAFESGFVSTQHMQSILDQTLEAAHSVISNFQT"
     gene            complement(1569..>1890)
                     /locus_tag="PK389_05190"
     CDS             complement(1569..>1890)
                     /locus_tag="PK389_05190"
                     /inference="COORDINATES: protein motif:HMM:NF015694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HQY23126.1"
                     /translation="AAERNQGYFPEERGMGISALEELIALKEAGHRAILFYCIQHNGI
                     SCLRLANNIDPRYGIRLREAVALGVECLAYRAHISLQEVVLGSRVPILLAEEPLGIKS
                     GNWK"
BASE COUNT          559 a          419 c          364 g          548 t
ORIGIN      
        1 tcatttaaaa tttgattgag ctgaaaattc gcttgttctt ggcgtttaag cgattcgtct
       61 attttaagca cagcctgttt ctgtctttta agcgccatac cataaaatat tgcaaaaata
      121 accagaataa tcgctaaaga catcacaaag tggacatcta tcgaatttaa tatttccatt
      181 tttaagaata cctcgcaaaa cctatattta agacaatctc atgttcatga ttctcacgcg
      241 gttgcatttc gacacatcac tcgtttaaga tttaaatcgc aacactcgcg aaaggatcat
      301 tttgatggac tattctacca agttatatgc tcgggcacaa actgttattc cgggtggggt
      361 taattcccct gtgcgcgcct ttaaaggcgt tgggggaacg cccgtcttct ttaaatctgg
      421 gaatggagcc tatctttttg acgtggaagg caaaaaatat attgattatg tcggttcttg
      481 gggtccgctt atccacgggc atgcgcatcc tcagattcta aaagctctgg aagccgccct
      541 accacaaggc ctaggatttg gtgctccaac tgaaattgaa gttgaaatgg cagaattaat
      601 caccgaactg atgccatcga ttgaacaagt acggatgtgt aactccggta ccgaagccac
      661 acaaagcgcc atccgattag ccagaggatt tacaggacga aacaaaatct taaaattcca
      721 gggcggttat cacggtcata ccgatagttt gctgattaaa ggcggttctg gattattaac
      781 attgggcata cctgattcgt taggtgtacc agaagctttt acaaacgaaa ccttaattgc
      841 accctttaat aatttagccg cagttaaaac catttttgaa aagtttggag atgaaattgc
      901 tgcgattatt gtagaaccta tcgcttgtaa catgaactgc attccacctt tgcctgattt
      961 tttaaccggt ttgaaagcac tctgtcatca atataaaagt cttctgatct ttgatgaagt
     1021 gatcaccgga tttagagtcg cactcggtgg cgcacaagca ctttatcaaa taacacctga
     1081 tctcacgtgt ctcggtaaaa ttatcggtgg cggattacct gttggtgcat ttggtggaag
     1141 acgcgatatt atggcctatt tagcacccca aggacccgtc tatcaagccg gtacattatc
     1201 cggaaaccct atcgctttaa ctgcagggtt cacaacattg cagttactca gagaacccgg
     1261 cgtttatgaa agcttaaaca aggcaacaac ctatttgatg aatggcttaa aaaatattgc
     1321 agaacagttt aatttaccct tacaaacaca tcaggtgggt agcctgtttg gcctgttttt
     1381 tacgacagaa aaaacgataa cacatttcga acacgttgaa caatgcaata ttgaacaatt
     1441 taaaaaattt tttcatggta tgttacagaa aggtgtttac ttagcaccct cggcttttga
     1501 atctgggttt gtatcaaccc agcatatgca atcaatcctc gatcagacat tagaagctgc
     1561 gcacagcgtt atttccaatt tccagactta atcccaagtg gttcttccgc caacaaaatg
     1621 ggcacccgag atcccaaaac cacttcctgt aaactaatat gggcgcgata agctaagcat
     1681 tctacgccca aggcgacagc ttctctcaag cgaataccat accttggatc aatattattt
     1741 gccagacgca aacaggaaat accgttatgc tgaatacaat aaaacaagat cgcacgatgt
     1801 cctgcttctt ttaacgctat cagttcttct aatgcagata ttcccatacc tctttcttcc
     1861 gggaaatacc cttggtttct ttcagccgca
//
LOCUS       DAORVT010000243         1889 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_67809_length_1889_cov_2.718648, whole genome shotgun sequence.
ACCESSION   DAORVT010000243 DAORVT010000000
VERSION     DAORVT010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67809_length_1889_cov_2.718648"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1284
                     /locus_tag="PK389_05195"
     CDS             <1..1284
                     /locus_tag="PK389_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HQY23127.1"
                     /translation="VDIGLDRAAFLHVADIRTSNQEGDTTKPIEKLLYEGNSILVQVI
                     KDAIGTKGARLSTQISLAGRLLVYLPKESHIGISQRIEDDSERELLREKLQQILPVDE
                     KGGYIIRTMAETADEIDLRADLEYLHKLWRDIQQQSVTITAPILLYQDLDLSHRVLRD
                     YVNENTALILVDSRENYQKLLKFAQSYMTFIAERITLYTGDRPLFDLYGVEEEIEKSL
                     AKRVNLKSGGYLIIDQTEALTTMDVNTGGFIGVRNFDDTIFKTNLEAAQVIARQLRLR
                     NLGGIIIIDFIDMDSEEHKNAVLTEFNKALLKDRTRITVNGFSALGLVEMTRKRTRES
                     LAHILCESCPTCQGRGEIRTAQTICYEILRELLRESRQFEANEFRILASQQVIDLFLD
                     EESQSLAQLGDFIAKPISLQVEESYTQEQYDVILM"
     gene            complement(1339..>1889)
                     /locus_tag="PK389_05200"
     CDS             complement(1339..>1889)
                     /locus_tag="PK389_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HQY23128.1"
                     /translation="TRSSEPWGFYNIVLQHRINPKTLLVLHHVYGHAGGVLLNNLKYT
                     NITKDAEWFSAIAHLYYDLTDNLSAGVRAEWYRDEAGFRNPSPFRIAAATNNVNGTPV
                     SYAGNLSSVTITPADYYAATVGLNWKAAKTLKLKWGFLKKLNIRPNIRYDRVDAYHAA
                     AYRPFAGNKDQILFSLDFLLPF"
BASE COUNT          581 a          363 c          418 g          527 t
ORIGIN      
        1 gtcgatatag gtcttgaccg ggcagcattt ttgcatgttg cagatatccg gacttccaat
       61 caagagggag atactactaa gccaattgag aaactgttat acgagggcaa cagcatcttg
      121 gtgcaagtga tcaaggatgc gattgggaca aaaggcgcgc gactatccac acaaatcagc
      181 ttggcaggcc gcctattggt ttatttgccc aaagaatctc acatcggtat ttctcaacgt
      241 attgaagatg acagtgaacg tgagttgttg cgtgagaaat tacaacaaat tctacctgta
      301 gatgaaaaag gcggttacat catcagaaca atggctgaaa cagcggatga aattgattta
      361 cgtgccgatc tcgagtattt gcacaagttg tggcgcgaca tccaacagca atcagtgact
      421 attacagcgc ccattttgct ttatcaagat ctggatttaa gtcaccgagt attacgagat
      481 tatgtgaatg aaaatactgc cctgatactg gtagattctc gggagaatta tcaaaaactt
      541 ttgaaattcg cgcaaagcta tatgacattt atcgctgaac gtataacttt atataccggg
      601 gatcgcccat tatttgattt gtatggcgtt gaagaagaaa ttgagaaatc tttggccaag
      661 cgcgttaatt taaaatccgg tggatacctg attattgatc agaccgaggc gctgaccacc
      721 atggacgtta acacaggcgg atttatcggt gttagaaatt tcgacgacac catctttaaa
      781 acgaatcttg aagccgccca ggtaatcgca aggcagcttc ggttgcgtaa tctgggtggc
      841 attatcatta ttgattttat cgatatggat tctgaagaac ataaaaatgc tgtattgact
      901 gaattcaata aagctttgct gaaagatcgt acgcgcatta cagtcaatgg atttagcgca
      961 cttggattgg ttgagatgac gcgtaaacgg actcgggaaa gtctcgcgca tatattgtgc
     1021 gaatcctgcc cgacctgtca aggacgaggg gaaatcagaa cggcacaaac aatttgttat
     1081 gagatcctac gtgaactgct aagagaatct aggcagtttg aagccaatga attccgtatt
     1141 cttgcatcac agcaagtaat tgatttgttt cttgatgaag aatctcagag cttggcacaa
     1201 ttgggtgatt ttatagcaaa gccgataagc ctacaggttg aggaatccta tacccaagaa
     1261 caatatgatg tgattttaat gtaaattatc acggaatgga gcctgagctg tattgattca
     1321 gcagtattca cttaaatttt agaaaggtag caagaaatca agtgaaaata aaatctgatc
     1381 tttgttaccg gcaaatggcc tataagccgc tgcatgatag gcatcgactc tatcgtaacg
     1441 aatattggga cggatattga gtttctttaa aaagccccat ttaagcttta gtgtcttagc
     1501 agctttccaa tttaaaccga cagtagctgc ataatagtct gcaggcgtaa tagttacact
     1561 actaagattg cctgcataac tgactggtgt accgttaaca ttattagtag cagcagcaat
     1621 ccggaacggg gatggattac gaaagccagc ttcatcacga taccattcag ctctgacacc
     1681 cgctgacaga ttgtccgtta aatcatagta taaatgtgcg atggcactaa accattcagc
     1741 gtcttttgta atattggtat atttcagatt gtttaacaaa actccacctg catgaccata
     1801 aacatggtgc aaaactaata gggttttagg attaattctg tgctgcaaca caatattata
     1861 aaatccccaa ggttcgctac ttcgcgtgg
//
LOCUS       DAORVT010000244         1880 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_68345_length_1880_cov_3.280548, whole genome shotgun sequence.
ACCESSION   DAORVT010000244 DAORVT010000000
VERSION     DAORVT010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68345_length_1880_cov_3.280548"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..1216
                     /locus_tag="PK389_05205"
     CDS             146..1216
                     /locus_tag="PK389_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009024107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HQY23129.1"
                     /translation="MFGFLKTKITPLVGVDISSSAIKILELSRAHGRYVVENYGIEFL
                     PSQSVVERNIKDVNKVGEAIRNLMKRMGCKLPFAAVAVAGSSVLTRVIQLNADLNELE
                     MADQIEVEADRYIPYPLEEVYYDFEVLDPVLKRPEFVNVLLAVTRIETLDMRVAAIVD
                     AGLQATVVDVEALAMERAFGLIGEHLPTKGAGSNVAVIDLGSTNSTLYVFKDRRLIYS
                     RDQAFGGKHLSDEIQRRYGLSAEEAVAAQKYGGLPEDYTTEVLTLFKETVIQQIGRAL
                     QVFFSSSEEEEIDYIVLAGGVAMLPSLDALIQEKLNIKTSIANPFVDMEIAERVNKNN
                     LMEEASALMLTCGLALRTFDNE"
     gene            1209..1820
                     /locus_tag="PK389_05210"
     CDS             1209..1820
                     /locus_tag="PK389_05210"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HQY23130.1"
                     /translation="MSKINLLPWREGLKKVRNRIFYLILCSLIAVNVLVTVLIGRFLN
                     YKVAIENTNIQYINRELKRAKEDVVEVKSLQHNKKALLERMKIIQSLQADRPSAVKLL
                     DLVPRVLPDGLYLVNLSRKDAVPEIEAMSTNEKEKTRLGNKKYSVVIQGVALTNGSIS
                     IFLKNLEKIKWVSEVKLNEVAISKDGDGLNFNLGFTQDLIEGE"
BASE COUNT          584 a          293 c          449 g          554 t
ORIGIN      
        1 ttagtataat accaaacata aaacgcactt tgccactgaa aggttagtag aattattggc
       61 ctttttacca gctaagacta gtcttaagtt ggaccgctct tttattttcg gtcacaatct
      121 tgcttcttga tgataggggt ttcctgtgtt tggtttttta aagactaaga taacgccatt
      181 ggttggggtg gatatcagtt catctgccat caagatatta gagctcagtc gagcgcatgg
      241 tcgctacgtg gtcgaaaatt acggtattga atttttgcct tcgcaaagtg ttgtcgaaag
      301 aaatattaaa gacgtcaata aagtcggtga agccattcgg aatctgatga agcgcatggg
      361 gtgtaagctg ccatttgcag cagttgctgt tgcgggatct tctgtattaa cacgcgtcat
      421 tcaactcaat gcagatctca acgagcttga gatggcagat caaattgaag tggaagcaga
      481 tcggtatatt ccttatccac tggaagaagt gtattacgat ttcgaggtgt tggatcctgt
      541 gctcaaacgt cccgaatttg taaatgtttt gttagctgta acccgaattg aaaccctcga
      601 catgcgggtg gcagctattg tagatgcagg attgcaggca actgtcgtgg atgttgaggc
      661 attagcgatg gagagagcct ttggattaat tggcgaacat ttgcccacaa aaggagcagg
      721 gagcaatgtt gccgtgattg atctaggatc caccaattcg acgctgtatg tttttaaaga
      781 tagacgttta atatacagtc gagatcaggc gtttggcggc aagcatctaa gcgacgaaat
      841 tcaaagacga tacggattat ccgcggaaga agcggttgct gcacaaaagt atggtggttt
      901 acccgaagat tatacaacag aggtattaac gctatttaaa gaaacggtga ttcaacagat
      961 tgggcgtgca ttacaggtct ttttctcttc gagcgaagag gaagaaattg attatattgt
     1021 attagcaggc ggtgttgcca tgttgcctag cttagacgca ttaattcaag aaaaattaaa
     1081 tattaaaaca tctattgcta atccatttgt ggatatggaa atagcagagc gtgtaaataa
     1141 aaataattta atggaagaag cttcagcatt aatgttgacg tgtggacttg ccttaaggac
     1201 tttcgacaat gagtaaaatt aatttattac cttggcggga agggttaaaa aaagtcagaa
     1261 atcggatttt ttatctcatt ttgtgcagtt taattgccgt caacgtatta gtgacagtac
     1321 tgattggtcg atttcttaat tataaagtgg ctatcgaaaa tacaaacatt caatacatca
     1381 atagagagtt aaaaagagca aaagaagatg tggtggaggt taaatcacta cagcacaata
     1441 aaaaagcgct tttagagcgg atgaagatta ttcaatccct tcaagcagac aggccatctg
     1501 ccgttaaatt gttggatttg gtacccagag tgttaccgga tggcttgtat cttgttaatt
     1561 tatcacggaa agatgctgtg ccagaaatag aggcaatgtc tacaaatgaa aaagaaaaaa
     1621 ccaggctggg taataaaaaa tatagtgtag ttattcaagg ggtcgcttta acgaacggga
     1681 gcatttctat ttttctgaaa aacttagaaa aaataaaatg ggtttcagaa gttaagttaa
     1741 atgaagtcgc tattagtaaa gatggtgatg gtcttaattt taatcttggt tttacccaag
     1801 atttgatcga aggggagtag cgcgtggaaa tcgctggcgt taagattgat gaacttgatt
     1861 ttgaagaagt tggaagttgg
//
LOCUS       DAORVT010000245         1871 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_68921_length_1871_cov_3.175110, whole genome shotgun sequence.
ACCESSION   DAORVT010000245 DAORVT010000000
VERSION     DAORVT010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68921_length_1871_cov_3.175110"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1501
                     /gene="argS"
                     /locus_tag="PK389_05215"
     CDS             <1..1501
                     /gene="argS"
                     /locus_tag="PK389_05215"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011174781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HQY23131.1"
                     /translation="VCTSGSTRGNGIANTLQAQCRDPHLGVSPPQKSLKVILDFSSPN
                     IAKEMHVGHLRSTIIGDCLSKILAFMGHNVLCLNHVGDWGTQFGMLIAYLKETLPAIT
                     NPIPPNVDLGSLATWYQASKKRFDEDPVFKKQAQQEVVALQSGEETSKRAWEHICTIS
                     RTAYQHIYDLLDIRLIERGESFYNPLLASVIQQLEKKGLLMHSEGAKCIYLDGFTNRE
                     GDPLPLILQKSDGGYNYATTDMAALQHRIQNEQGDWLIYITDAGQALHFQMVFAAGEK
                     TGIFDPKKVRIDHVPFGLVLKPDGKKFQTRSGKTERLIDLLHTAIEHAKQKLKEHNPD
                     ISDSELEASAKVLGINAIKYADLSCHRLSDYVFSYDKMLQFEGNTAAFLLYAYVRIQS
                     IQRKVNVDIANLLANHTPLILEDPTEIALGLLVCQFNEALENCARELLPNRLTDYLYR
                     LAEKFHAFFHQCRVEGTPNQNSRLLLCFATAQVLGQGLLLLGLKPLERM"
     gene            1512..1835
                     /locus_tag="PK389_05220"
     CDS             1512..1835
                     /locus_tag="PK389_05220"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY23132.1"
                     /translation="MNFQLRMLIAAPIMIAAICARPSYALETDSESAYQKGLALYSKS
                     GIEKNSVTAAVYFKEAAEQGHLDAAFSLGVLYQNGEGVPTSSQEAIKWYTKAADQNHA
                     KAQFN"
BASE COUNT          579 a          403 c          369 g          520 t
ORIGIN      
        1 agtctgtacc agcggttcaa cccgtggaaa tggtattgca aacacccttc aagcacaatg
       61 cagagatcca catttaggtg tatctccccc tcaaaaatct ttaaaagtca tactcgattt
      121 ttcttctccc aacattgcca aagaaatgca tgtcggacat ttacgctcga ccattattgg
      181 agattgcctt agcaaaattt tagcctttat ggggcacaac gtcctttgtt taaaccatgt
      241 gggcgattgg ggtacacaat ttggcatgtt aatcgcctat ttaaaagaaa cactccctgc
      301 tattacaaat cccattccgc ctaatgtcga tttaggatcg ctggctactt ggtatcaagc
      361 ctccaaaaag cgattcgatg aagatcctgt ctttaaaaaa caagcgcagc aagaagtcgt
      421 tgccttgcaa agcggcgaag agacctctaa acgagcttgg gaacatatct gcaccatttc
      481 tcgtaccgca taccaacaca tttatgattt actagatatt cgtcttattg aaagaggcga
      541 gtctttttat aatccattgc tagcatctgt gatccaacag ttagaaaaaa aagggctttt
      601 aatgcactcc gaaggtgcga aatgcattta cctcgacggt tttacaaatc gtgaaggcga
      661 ccccctgccc ctcattttac aaaaatccga tggcgggtat aactatgcaa cgaccgatat
      721 ggctgcatta cagcatcgca ttcaaaacga acagggtgat tggcttattt acattaccga
      781 tgcgggacaa gctcttcact ttcaaatggt gtttgcagca ggcgaaaaga ccggcatttt
      841 tgatcccaaa aaagttcgaa ttgatcatgt gccttttgga ttggttttaa aacccgatgg
      901 caaaaaattt caaacccgtt caggcaaaac agaacgtctg attgatcttc tgcatacggc
      961 aatagaacac gctaaacaaa agctaaagga acataatccg gatatctctg actccgaact
     1021 agaagcctct gccaaagtac ttggtatcaa cgcaattaaa tatgccgacc tgtcgtgtca
     1081 tcgtttaagc gattacgtct ttagttacga taaaatgtta cagtttgaag gaaacactgc
     1141 agcattttta ctatacgctt atgttcgaat acaaagcatt caacgcaaag tcaatgtcga
     1201 cattgcaaac ttactcgcaa atcatactcc cctgatatta gaggacccca cagaaattgc
     1261 tttgggactt ttggtttgtc aatttaatga agccttagaa aactgtgcac gcgagctatt
     1321 acccaatcgt ttaaccgatt atttatatcg tctggctgaa aaatttcatg cgtttttcca
     1381 tcaatgtcgt gtagaaggaa cacccaacca aaacagtcga ttactgttat gttttgcaac
     1441 tgcacaagtc ttaggtcaag gattattgtt gttgggccta aaaccgttag aacgaatgta
     1501 ggagaatcaa tatgaatttt caactacgaa tgttaatcgc cgctcctatt atgattgctg
     1561 caatatgcgc acgcccctcg tatgcattag aaactgatag cgaaagtgct tatcaaaaag
     1621 gtttagcgct ttactcaaag tctggaatag aaaaaaacag tgtcactgct gctgtctatt
     1681 ttaaagaagc ggccgaacaa ggtcatctgg atgcggcttt tagtttaggc gtgctttatc
     1741 aaaatggcga aggcgtgcct acaagcagcc aggaagctat aaagtggtat acaaaagccg
     1801 ccgatcaaaa tcatgccaag gcacaattta actaaaatta tttattatat gaaggtattg
     1861 ggacagagcg t
//
LOCUS       DAORVT010000246         1859 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_69712_length_1859_cov_2.895233, whole genome shotgun sequence.
ACCESSION   DAORVT010000246 DAORVT010000000
VERSION     DAORVT010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69712_length_1859_cov_2.895233"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1420)
                     /gene="recN"
                     /locus_tag="PK389_05225"
     CDS             complement(<1..1420)
                     /gene="recN"
                     /locus_tag="PK389_05225"
                     /inference="COORDINATES: protein motif:HMM:NF008121.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HQY23133.1"
                     /translation="MLTHFFIQHLAVVEQLALNIQSGMTVLTGETGAGKSILIDALQL
                     ALGGRAESGIVRHSYPQAEISAVFELTRLPAVLDWLKEHALEAEGECIIRRTLTEDGR
                     SRAYINGRVVPISQLRELGEQLVNIHGQHQHQSLLKSEHQRTLLDAYAIAAVKDSKKT
                     KSSNLQHADLLKSVKINYHLYQTLLKEHQTLLSLQGQNDKLTLLQYQIQEIDELNLAQ
                     DELTTLDAEHKHLAHAEQWLFSCETALSALKNDANQDATTLLYQGLHQISTLKTQIAT
                     LTTAHELLNTAYIQLTEAIVEIENFKETLSSDPNRLMEIDKRLGQIHALARKHRIQPE
                     AILEHKKTLETEAKKLVHLHTHLEEITEKLASAEKAYQNAAIELSKSRQKAAVTLEQR
                     VTQCIQTLEMPNGHFKIAFSTPHENSKNRLVSSPHGIDDIEFLVSTNPGLALQPLRKI
                     ASGGELSRISLAIQVITTQKQST"
     gene            complement(1421..>1859)
                     /gene="ppnK"
                     /locus_tag="PK389_05230"
     CDS             complement(1421..>1859)
                     /gene="ppnK"
                     /locus_tag="PK389_05230"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="catalyzes the phosphorylation of NAD to NADP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HQY23134.1"
                     /translation="NDVVLYTGSIARMVDFEVYINDHFVLRQQADGIITATPTGSTAY
                     ALSAGGPILYPTLKALTITPLCPHTLSSRPIVVDSDSDIRLVVTQNNNIKPKLSCDGQ
                     SHFDLEASDQISIQKYPFELTLIHPKTHEYFSVLREKLGWSTQ"
BASE COUNT          475 a          337 c          422 g          625 t
ORIGIN      
        1 gggtcgactg cttttgggtg gtaatgacct gaatggctaa actaatacgc gataattcgc
       61 cgccagaggc aatcttgcgt agcggttgca aggcaagtcc tgggtttgtt gaaaccaaaa
      121 attcaatgtc atcgatgccg tgtggactag aaacaagcct atttttagaa ttttcatgcg
      181 gtgtagaaaa agcaatttta aaatggccat tcggcatttc taaagtttgg atacattggg
      241 taacccgttg ttctaacgtt acggctgctt tttgtcggct tttgctgagt tctatggccg
      301 cattctgata agctttttca gcacttgcca gtttttcggt tatttcttct aagtgcgtgt
      361 gaaggtgaac taatttttta gcttcagttt caagtgtttt tttatgttct aaaatagcct
      421 cgggttgaat acgatgtttt cgagcgagcg catggatctg gcctagacgt ttgtctattt
      481 ccatcagtcg attgggatca ctgcttaagg tttctttaaa gttttcgatt tctacaatag
      541 cctcggttaa ttgaatatag gcggtattca ggagttcatg cgctgttgtt agcgttgcta
      601 tttgcgtttt tagcgttgag atttgatgaa gaccctggta aagcagtgtt gttgcatctt
      661 ggttggcgtc atttttaagc gctgaaaggg cggtttcaca gctaaaaagc cattgttcgg
      721 catgtgctag gtgtttatgt tcggcatcta aagtggtgag ttcatcctgt gctaaattta
      781 attcatcgat ttcttgaatt tggtattgca aaagcgttag tttatcattt tgtccttgaa
      841 gactgagcag cgtttgatgt tcttttagaa gcgtttgata taaatgataa tttattttga
      901 cggactttaa taaatccgcg tgttgtaggt tgctagattt tgtttttttt gaatctttta
      961 ctgctgctat cgcatatgca tcgagcaagg tgcgttgatg ttcggatttt aataaagact
     1021 gatgttgatg ctgtccatga atattgacca attgttcgcc taattctctt aattggctga
     1081 taggtacgac gcggccatta atataagccc gagagcgacc gtcttctgtt aatgtgcgtc
     1141 gaataatgca ttcaccctct gcctcaaggg cgtgttcttt tagccagtct aagactgctg
     1201 gaagacgcgt taattcaaaa acggcagaga tctctgcttg tggatagcta tgtcgaacaa
     1261 taccactttc agctcgtccg cctaatgcta attgtagggc atcaatcaga atggactttc
     1321 cggcacccgt ctcacctgtt aaaacagtca taccagattg gatattcaag gctaattgtt
     1381 caacgaccgc taaatgttga ataaaaaaat gggttagcat ttattgtgtg ctccatccca
     1441 gtttttcgcg taataccgaa aaatattcat gggttttggg gtggatgagt gttagttcaa
     1501 agggatattt ttgaattgaa atctgatcac ttgcttccaa atcgaaatgc gattgcccat
     1561 cgcaactcaa ttttggctta atattgttgt tctgtgtaac cacgagacga atatcgctgt
     1621 cgctatctac aacgataggt ctactgctta aggtatgtgg acaaagtggc gtaatggtta
     1681 aggctttaag tgttggataa agaataggac cacccgcaga taatgcataa gcagttgaac
     1741 ctgtcggtgt tgcagtaata atgccatcgg cttgttgacg caacacaaag tgatcattaa
     1801 tgtacacttc aaaatcaacc attcgtgcaa tgctacctgt atatagaaca acatcattt
//
LOCUS       DAORVT010000247         1847 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_70550_length_1847_cov_3.867188, whole genome shotgun sequence.
ACCESSION   DAORVT010000247 DAORVT010000000
VERSION     DAORVT010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70550_length_1847_cov_3.867188"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1654)
                     /locus_tag="PK389_05235"
     CDS             complement(<1..1654)
                     /locus_tag="PK389_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQY23135.1"
                     /translation="MLTKGSIENLLLSEDEQNQQLSEWNNTVTSYPLGDCMHVLFSQS
                     VVQAPNQVALRMERGKSYTYQEIDKLSDHVANFLMNKLRPSSLIAVLLQPSLEFVVNV
                     LGILKAGCTYLPIETKSSEERLKFILTDCKASALITTKTLKEKYSQFINQTFTFEDYQ
                     NVVYTEIPTTNNAAEQIAYVRYSLTEKKSKAIAFSHRSLVNAIHARSLLFPVLNKIAL
                     LHSVESDLTITSLFWAFYLCGTVVIPECLNPWDIESTLSLIEQEAITHLLYCPGPYDA
                     LLEQAKGELKSLEVVSLSGETIFPNLLEKHLKSASHTSLYNEYKVPGIFSLASVTKLY
                     DKNNKEHQEITLGRPIANTHIYLFNEKKEFVPVGVKAEIYVGGLGLGSDLNSAIEDDF
                     VKHPFLPSEKLYRTGDIACYLEGGFIEYITSRDRQVKHLEQQVDQVEIENILREHSSV
                     ADAVVMLSTGQNGVKRLVAYIVWAEKTDPDSELEILKSCLHKQLPSYKIPQIFMSVGS
                     FEFTLSGKRDISTLPVPLQENRGDGFKKPHTEIEKCLAGIWSE"
BASE COUNT          555 a          368 c          313 g          611 t
ORIGIN      
        1 gttcgctcca gatgcccgcc aaacacttct ctatttcagt atgtggcttt ttaaagccat
       61 cgcctctatt ttcttgcaat ggaacaggca acgttgagat atcacgttta ccagataaag
      121 taaattcgaa tgaacccaca ctcataaaaa tttgtgggat cttataactt ggtaattgct
      181 tgtgcaaaca actctttaaa atttctagtt cggaatcagg atcggttttc tcagcccata
      241 caatataagc aactaaacgt ttaacgccat tttgacctgt acttaacata acaaccgcat
      301 ctgccacaga agaatgctca cgtaaaatat tttcaatttc tacttgatct acttgctgtt
      361 cgaggtgttt tacctgccga tctctagaag taatatactc aataaaacca ccttctaaat
      421 agcaagcaat atcacctgta cgataaagct tctcagaggg caaaaaagga tgtttaacaa
      481 aatcatcctc gatggcagaa ttcaaatcgg aacccaatcc aagtcctcca acgtatattt
      541 ccgctttcac gccaacaggc acaaattctt ttttttcatt gaaaagataa atgtgagtat
      601 tggcaatagg acgacctaac gttatctcct ggtgctcctt gttattttta tcgtacagtt
      661 ttgttacgga cgcaagacta aaaatacccg gcaccttata ttcattatat aaacttgtat
      721 gagaagccga ttttaaatgt ttttctagta aattgggaaa gatcgtttca ccacttaaac
      781 taactacttc taaagatttt aactctcctt tggcctgttc taataaggca tcataaggtc
      841 ccgggcaata aagtaaatgg gtaattgctt cttgttctat taacgataaa gttgactcta
      901 tatcccaggg gtttaggcat tctggaatga ctacagtacc acacaaataa aaggcccaga
      961 ataagctggt gatagttagg tccgattcaa cggaatgtaa taaagcaatt ttgttcaaaa
     1021 ctggaaaaag taaacttctt gcatggattg cgtttactag actccggtga gaaaaagcga
     1081 tagccttaga ttttttttct gtcagtgaat accgaacata cgctatttgc tctgccgcat
     1141 tgttggtggt gggtatctcc gtatatacaa cgttttgata gtcctcgaaa gtaaaagttt
     1201 gattaataaa ttggctatat ttttctttta aggtttttgt agtgattaat gcacttgctt
     1261 tacaatcagt cagtataaat tttaatctct cctcagaact cttggtctct atgggtagat
     1321 aggtacaacc cgcttttaaa attcctaata cattcaccac aaactctagg gagggttgta
     1381 ataagactgc aataagactg ctaggcctaa gcttgttcat taaaaaattg gctacgtgat
     1441 cgcttagttt gtctatttct tgataggtat aagatttacc tcgttccata cgcaaagcta
     1501 cctgattagg agcttgcaca acggattgcg aaaacaagac gtgcatacaa tcgcccaatg
     1561 gataggacgt taccgtatta ttccattcgc ttaactgttg attttgttcg tcttcgctta
     1621 agagtagatt ctcaatactt ccctttgtta acatggtgac tttccattaa ttcacgggta
     1681 gtgataaatg acaaacagcg agttaaatat tccccactca atgcctaatt tgcgtatgct
     1741 tcttcaagtt cctcaactaa gttttcgtca ttttttatta taaaatcatt cttttcatcc
     1801 agtagcaata cttttggttt aaatgtctta tattcttttt cttctaa
//
LOCUS       DAORVT010000248         1842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_70873_length_1842_cov_3.651931, whole genome shotgun sequence.
ACCESSION   DAORVT010000248 DAORVT010000000
VERSION     DAORVT010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70873_length_1842_cov_3.651931"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /gene="tsf"
                     /locus_tag="PK389_05240"
     CDS             complement(<1..297)
                     /gene="tsf"
                     /locus_tag="PK389_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HQY23136.1"
                     /translation="MTITAAQVKELRERTNAGMMDCKKALIASGGDIEKAIAAMQEAG
                     QANAIKKAGRTASEGTIIVLASSDGKRGVLVEVNCETDFVAREEKFKQFAKTTGE"
     gene            complement(302..1084)
                     /gene="rpsB"
                     /locus_tag="PK389_05245"
     CDS             complement(302..1084)
                     /gene="rpsB"
                     /locus_tag="PK389_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HQY23137.1"
                     /translation="MTISVSMREMLEAGVHFGHLTRFRDPSMIPYIYCIQNGVHIINL
                     EKTLPLFRQALKFVQKIASKNGKILFVGTKRSAKVLVAEYAQKSGMPYVDHRWLGGAL
                     TNYKTIKQSVRRLKDLEAMRQDGTLKQLTKKEGLRLMRELEKLERGIGGIKEMGGLPD
                     AIFVLDVGNEKIAIKEANCLSIPIIGVVDTNHNPAGVDYPIPGNDDAMRAIELYLKSV
                     ADTILFAKQSEKNALSAKFTEEFIEVDETIERSLDSKNSAEG"
     gene            1287..>1842
                     /gene="map"
                     /locus_tag="PK389_05250"
     CDS             1287..>1842
                     /gene="map"
                     /locus_tag="PK389_05250"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014997567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HQY23138.1"
                     /translation="MSHIKIKSDDEIVKMRVAGRLAAEVLEIIEPHVKAGVTTLELND
                     ICHNYIVNEQNAIPACLNYRGGGITPFPKSVCTSVNHVVCHGIPNDRKLKSGDILNID
                     VTVIKDGYHGDTSKMFLVGECAILPKRLTRITQECLYLAIKAVKPGAKLGDLGAIIQK
                     HAESNQYSVVREYCGHGIGTEFHEE"
BASE COUNT          528 a          415 c          350 g          549 t
ORIGIN      
        1 ttcacccgtt gttttggcaa attgtttaaa cttttcttct ctcgcaacaa aatccgtctc
       61 acaattgact tcaactaaaa caccgcgttt accgtcagaa gatgccaaaa caataatggt
      121 gccctctgat gcagtacgtc ctgctttttt aatcgcatta gcctgcccgg cttcctgcat
      181 tgctgcaatg gctttttcaa tatcgccacc cgatgctatt aatgcttttt tacagtccat
      241 catacctgca ttggtacgct cgcgaagttc tttaacctgt gctgctgtaa ttgtcatatg
      301 tttaaccttc tgcgctgttt ttagaatcaa gacttctctc gatggtttca tccacctcaa
      361 taaattcttc tgtaaatttg gcacttaacg catttttttc agattgcttt gcaaacaaaa
      421 tggtatctgc cacactctta agatacaatt caatcgcacg catcgcgtca tcattacccg
      481 gaatcggata atctacacct gcaggattat ggttagtatc gacaacacca ataatgggaa
      541 tactcaaaca attcgcttct ttaatcgcaa ttttttcatt gcccacatcg agtacaaaaa
      601 tggcatcggg taatccaccc atttctttaa tcccgccgat acctcgctct aatttttcta
      661 attctcgcat caagcgcaaa ccttcttttt ttgttaattg ttttaaagtt ccgtcttgac
      721 gcatggcttc taaatccttc agccgacgaa cagattgctt gatggtttta taattggtga
      781 gtgcgccgcc cagccaacgg tgatccacat agggcattcc agatttttgc gcatattctg
      841 cgacaagcac ttttgcagaa cgcttcgtac ccacaaacaa aatttttcca tttttagaag
      901 caatcttctg aacaaattta agggcttgac gaaacaacgg aagggtcttt tctaaattaa
      961 taatgtgaac cccgttttga atacaataaa tgtatgggat catgctaggg tcgcgaaagc
     1021 gagttaaatg accgaaatgg acgccggcct ctaacatttc acgcatactc accgaaatag
     1081 tcatatcgaa ctcctataag ttttagggtt aagcctccat gcatcccata atccaaccct
     1141 gcatgtttct aaaaacctgc ctggcaccca agatcatgtg acgatgcatg tgtgtaatta
     1201 cctcgcctct agaaaacgtt tctagacact ggagaagaag cgttataaca tggtaaagct
     1261 ctaactggca ataaggacgc taacttttgt ctcacattaa aatcaaaagc gatgatgaaa
     1321 ttgtaaaaat gcgcgtggcg ggtaggctgg ctgcagaggt gctcgaaata atagagcccc
     1381 atgttaaggc cggggtgacg acgctcgaat taaacgatat ttgtcataat tatatagtca
     1441 acgaacaaaa tgctattcca gcctgcttaa actatcgtgg cggtggcatt acgccttttc
     1501 caaaatctgt ctgtacatca gttaaccatg ttgtttgcca cggtatccca aacgatcgaa
     1561 aattaaaatc tggcgatata ctcaacattg atgtgactgt tatcaaagac ggctatcatg
     1621 gagatacgag caaaatgttt ttggtgggag aatgcgccat tttgcctaaa cgtttaacgc
     1681 gcataacgca agaatgtctt tatcttgcaa ttaaagctgt taagccaggt gcaaaactgg
     1741 gggatttggg tgctatcatt caaaaacatg cagaatcaaa tcagtattca gtcgtacgtg
     1801 aatattgtgg gcatggtatc ggtacagaat ttcacgagga ag
//
LOCUS       DAORVT010000249         1827 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_71959_length_1827_cov_3.671558, whole genome shotgun sequence.
ACCESSION   DAORVT010000249 DAORVT010000000
VERSION     DAORVT010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71959_length_1827_cov_3.671558"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="PK389_05255"
     CDS             <1..379
                     /locus_tag="PK389_05255"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006280502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HQY23139.1"
                     /translation="RRKLFQSRFLQGCWMLMTPSGFIALLAGWFVTEIGRQPYTVYGI
                     IRTSESVSPAIIGSQVACSLLAFVVMYTFLFGAGSYYILKLVYKGIAVTGDKEQFYKH
                     GLEASILESSSIEVDNKKGDNHV"
     gene            372..1394
                     /gene="cydB"
                     /locus_tag="PK389_05260"
     CDS             372..1394
                     /gene="cydB"
                     /locus_tag="PK389_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010423624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HQY23140.1"
                     /translation="MFDFSSTMDLPMIWGLLIATAILLYILLDGFDLGIGILFPFAPS
                     DKCRDRMMNSIAPFWDGNETWLVLGGGGLFAAFPLAYAILLPAFYIPIIAMLLGLILR
                     GVAFEFRFKSEGKLRYLWDYVFHFGSLGAAFCQGMILGGFVQGVTVNGRNFSGGPFDW
                     ATGFSVMTGMAVVFGYALLGSTWLVMKTEDITQAWARKVAAYVLGFVGIFMALVSMVM
                     PLMNAPIKNFWFSLPNFYYLFPIPLLTTALFMMLWIDLHREKREYRPFLLSIGIFFMG
                     YLGLGMSLYPWIIPFHYTLRDAAASGPGLSLMLVGVVPLLPLILTYTGYSYYVFRGKT
                     NHEDSY"
     gene            1378..1533
                     /locus_tag="PK389_05265"
     CDS             1378..1533
                     /locus_tag="PK389_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23141.1"
                     /translation="MKIHIKYSIQTWLAAHPRARQWLWLITLWLVGLLLVSVLTYPLK
                     LLINIVK"
     gene            1631..>1827
                     /locus_tag="PK389_05270"
     CDS             1631..>1827
                     /locus_tag="PK389_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HQY23142.1"
                     /translation="MIILAAGNGLRMCSTLPKVLHPIAGVPILERILLTVSHLSPKQV
                     IVVHGAHGDQLKNVFVNHKNLT"
BASE COUNT          437 a          329 c          420 g          641 t
ORIGIN      
        1 tcgtcgcaaa ttatttcaaa gtcgtttttt gcagggatgt tggatgttga tgacaccctc
       61 tgggtttatc gcattgttgg caggttggtt tgtgactgaa attggtcgtc aaccgtatac
      121 cgtctatggc attattcgca caagtgagtc tgtgtctcct gcgattattg gatcacaagt
      181 tgcctgctca ctcttggctt ttgttgtgat gtacactttt ctgtttggcg caggaagtta
      241 ttacatattg aagcttgttt acaagggaat cgctgtgaca ggtgacaaag agcagtttta
      301 taaacatggt ttagaagctt caattttaga atcctcgagt atcgaagttg ataataaaaa
      361 aggagacaac catgtttgat ttttctagca caatggattt acccatgatt tggggtttgc
      421 tgattgcgac agctattttg ctttatatat tactcgatgg ttttgatttg ggaattggta
      481 ttttgtttcc tttcgcgccc tccgacaaat gtcgcgatcg catgatgaat tctatcgcac
      541 cattttggga tggaaacgaa acctggttag tattgggtgg cggtggatta tttgcggctt
      601 ttccgttggc ctatgcaata ttattgccgg ccttttatat tcccattatt gcgatgttgt
      661 taggtcttat actacgtggt gttgcatttg agtttcgctt taaatcggaa ggaaaattgc
      721 ggtatttgtg ggattatgtt tttcattttg gatcgctcgg cgctgctttt tgtcagggaa
      781 tgattttggg gggatttgta caaggtgtca ctgtcaatgg tcgcaatttt tccgggggtc
      841 cttttgattg ggcaacagga tttagtgtga tgacaggtat ggctgttgtg tttgggtatg
      901 cgctcttagg ttctacctgg cttgtgatga aaacggaaga tatcacccaa gcatgggcgc
      961 gtaaggtggc tgcttacgtg ctcggctttg ttggtatttt tatggcttta gtgagcatgg
     1021 tgatgccgct tatgaatgca cccattaaaa acttttggtt tagccttccc aatttttatt
     1081 atttattccc tattccttta ttaacgactg cattatttat gatgctgtgg attgatctac
     1141 atcgagaaaa acgagaatac cgcccatttt tattgagcat tggcattttt tttatgggct
     1201 atctggggct gggaatgagt ctttatcctt ggattatccc cttccattat acccttcgag
     1261 acgcagctgc ttcgggtccc ggtttatctt tgatgttagt gggtgttgtc ccacttttgc
     1321 cactcatcct aacgtacacg ggttattctt attatgtttt tcgcggaaaa acaaaccatg
     1381 aagattcata ttaaatattc tatacaaaca tggttagcag cgcatccgag agcaaggcag
     1441 tggctgtggc ttattacgct atggcttgtt ggattattgc tcgtatccgt attaacgtat
     1501 cccctaaaat tattaattaa tatagttaaa tagttgattt tgtagattag ctttattgaa
     1561 tgatctgaaa atgataaaat gctcaaactt aaaccatagg gttaaacgag ggcatatgaa
     1621 taacctctct gtgataatac ttgccgcagg caatggcctt cgtatgtgtt ctacgctacc
     1681 aaaagtgctc catccaattg ctggcgtgcc cattcttgag cggatacttt taacagttag
     1741 tcacttaagc ccaaaacaag tgattgtggt acacggagca catggcgacc aacttaagaa
     1801 tgtatttgtt aaccacaaaa accttac
//
LOCUS       DAORVT010000250         1821 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_72348_length_1821_cov_3.391846, whole genome shotgun sequence.
ACCESSION   DAORVT010000250 DAORVT010000000
VERSION     DAORVT010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72348_length_1821_cov_3.391846"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="PK389_05275"
     CDS             complement(<1..528)
                     /locus_tag="PK389_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030388.1"
                     /note="This protein performs the mismatch recognition step
                     during the DNA repair process; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HQY23143.1"
                     /translation="MMQQYLRIKAEHRDHLLFYRMGDFYELFFEDAKRASELLNITLT
                     ARGQNQGNPIPMAGVPYHAAENYLAKLVRLGETIAICEQIGDPSTSKGPVERRVVRIL
                     TPGTLTDEALLNESEDNYIMALHVNAEKTNPNVLYGFAYLDLASGRFYISETATESNL
                     LQELERIKPSEILISE"
     gene            complement(565..>1821)
                     /locus_tag="PK389_05280"
     CDS             complement(565..>1821)
                     /locus_tag="PK389_05280"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HQY23144.1"
                     /translation="THITVKDITLKNNAQRLQIKGDFYYGKQGLNSNLHLAAFFVPKQ
                     ALTIKDLNLNISGKIGNPLLLKGFAYSGAGKFQVDGTLRFNTEKRLSLNLSGKNLQIY
                     NTQNMQIIGSPTLTLSLNNNTLFVDGTLLIHKAYLAMQDQKNVTLLSKDIVLTNSSEP
                     ATRTNGFRIIPNLYVVIENNLHFDGYGIIGTVGGKLTINERPDGLLTGNGKLTIKEGK
                     YRLQGATRYIHRGHLLFPPGTLLKDPLLDILISQNRVQQGNITNSNIGLYVQGTLLHP
                     IYHPYSSDNNLKGADILSRLGFGQSEVSGDENQRQLFAQTAFLFSGTANPFVDFLQKN
                     LKLEEFNLESKPTNKTFYTQGGADTVLVVGKSLSQKLYLQYLQSIIEPIATIRLKYFL
                     SRFFTASAETGTEGIGGDLTFSMEKN"
BASE COUNT          516 a          318 c          367 g          620 t
ORIGIN      
        1 ctctgaaatt aaaatttctg agggcttaat gcgttctagt tcttgtaata aattggattc
       61 tgttgcagtt tctgagatat aaaaacgacc acttgccaag tctaaataag caaagccata
      121 gagcacattt ggatttgttt tttcagcgtt aacatgcagc gccataatat aattgtcttc
      181 actttcgttt aagagcgctt catctgttag agtgcccggt gttaaaatac gaaccacacg
      241 tcgttctaca gggcctttgc tggtactggg atcgcctatt tgttcgcaga tagcaattgt
      301 ttcaccaaga cgcactaatt ttgcgagata gttttcggca gcatgatagg gcactcctgc
      361 catggggatt ggattccctt ggttttgtcc acgcgctgtc agtgtgatat ttaaaagttc
      421 tgatgcgcgt tttgcatctt caaaaaatag ttcgtaaaaa tcgcccattc tataaaataa
      481 cagatgatcg cgatgttctg cttttatacg caaatattgt tgcatcatgg gcgtgtgttc
      541 gattggttga gtgctgtttt tcacttagtt tttctccatt gagaaggtca gatcgccgcc
      601 tatgccttct gttcctgttt cagcgctggc agtaaaaaat cggcttaaaa agtattttaa
      661 acgaattgtc gcgatgggtt ctataatact ttgtaaatat tgtaaataca atttttgaga
      721 aagagatttt ccaaccacaa gaacggtatc agcaccgcct tgagtgtaaa atgttttatt
      781 ggttggtttt gattctaaat taaattcttc tagttttaag tttttttgga gaaaatcaac
      841 aaagggattt gctgtacctg aaaataaaaa agccgtttga gcgaaaagtt gtcgttgatt
      901 ttcatcacca gagacttcgc tttgtccaaa acccaaacgc gataaaatat cggcaccttt
      961 taaattatta tcgctagaat aggggtggta aatgggatgt aacaaagtac cctgtacata
     1021 gagcccaata ttactattgg taatattgcc ttgttgtacg cgattttgag atatcaagat
     1081 atctaagagc ggatctttga gcaacgtgcc tggcggaaac aataaatgac cacggtgaat
     1141 gtatcgcgtt gctccttgta aacgatattt accttcttta atggtcagtt ttccattacc
     1201 tgttaggaga ccatcaggcc gctcgttaat agtcagtttt ccacccactg ttccaatgat
     1261 accgtaccca tcaaaatgta aattattttc aatcaccaca taaagattgg ggataattct
     1321 aaagccattc gtcctggtcg caggttctga ggaatttgta agaacaatat ctttagaaag
     1381 caaggtaaca tttttttgat cttgcatcgc aaggtaagct ttatggatta agagagtgcc
     1441 atcaacaaac agcgtattat tattaagact taaggttaat gtcggtgatc ctattatttg
     1501 catattctgg gtattataaa tttgtagatt ttttccgctg agattcagtg aaagtctttt
     1561 ttcagtattg aatcgcaggg tgccatctac ttgaaatttg cctgctccag aataagcaaa
     1621 accttttaat agtaagggat ttccgatttt tcctgaaata ttgagattta gatctttaat
     1681 cgttagtgct tgttttggca caaagaaagc agctaaatgg aggtttgaat ttaagccttg
     1741 tttaccataa taaaaatctc ctttgatttg taagcgttgg gcgttgtttt ttagtgtaat
     1801 gtcttttact gtaatatggg t
//
LOCUS       DAORVT010000251         1812 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_72928_length_1812_cov_5.066022, whole genome shotgun sequence.
ACCESSION   DAORVT010000251 DAORVT010000000
VERSION     DAORVT010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72928_length_1812_cov_5.066022"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(522..1592)
                     /locus_tag="PK389_05285"
     CDS             complement(522..1592)
                     /locus_tag="PK389_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HQY23145.1"
                     /translation="MDNFTLFDQPLCDKALVQNVELEEFDEASRYAWLSLITRETYEE
                     AKKQLKGSAFYQDNQLVEDITTGLDLSYDQFDLKRQKELNLYKGDLSRVVSEKRLKSY
                     LMPEQLDAWVNCIAVNSDAPVLVYVQKVTDTAATINIRYRGAAHTPENVKFMVNIKGA
                     ANDEKPKREIPIKKGGSFIFNITRASSNHEIRVVVNGESMSAEDTYIIKGDKRKHPPP
                     PPASLRTQIKIDSIECSNAHVTAPPRNAFDGDENSAWNAGSHGQHWIRAHFQNPVDLT
                     EIKLIISQAPPGHTKHEVYVGLEDGNRMLCAVFEGETSDRGILRKNVKVKRVLSIEVK
                     TISSPSWIAWFEIEPIGRLSSI"
BASE COUNT          518 a          368 c          315 g          611 t
ORIGIN      
        1 tatgccaagt ttgcgactga gaatttagcg tttgcagcgt caagaatcga gacaggaaaa
       61 gatcgaaaag taattaaaga tgctaaccag agctataagc ctgctaactt tgagttaatt
      121 ggctcaccag atcggataaa tctggtaact ggcatatcat agttctgttt tgaaattcaa
      181 tattttgaga tttatatgcg acaaatggcc ttattgaatt gcccggcttg tttatagcgc
      241 aattttgtct caagtccatt tgaacttttg ggtatattaa aaattaaatt tgttcaccat
      301 cagtgacctt tgaaaagtcc tgttttcaga aaattataat taaaacagac tttgaattaa
      361 ggattcgtat aagagctttt gcaaagatct ccatgaatat tctgaaactc gtacaccgta
      421 cacaagtaag aggtgattac tctctgcagt tatttactaa ggggtggcac aaaagcaaat
      481 agccttaacc ccttagcatt tgatattgac aatcgaccca actatatact actaagacgg
      541 ccaatcggct caatttcgaa ccaagcgatc caagatggcg aggatatcgt ttttacttca
      601 atcgaaagaa ctctttttac tttaacattc tttcgcaata tccctctatc ggaagtctca
      661 ccttcaaata cggcgcacaa catccgattg ccatcttcaa gtcctacata tacttcatgt
      721 ttcgtatggc ctggaggtgc ctgagatatt attaatttta tctcggtcaa atccacagga
      781 ttttgaaaat gcgctcttat ccaatgttgt ccgtgacttc ccgcgttcca agccgagttc
      841 tcgtccccat cgaacgcatt tctcggcggt gcagtaacat gtgcatttga acattcgatg
      901 gaatcgattt ttatttgagt acgcaagctc gcgggaggag gaggaggatg tttccgttta
      961 tcacctttaa tgatgtaagt atcttcagcg ctcatcgatt cgccgttaac gacaactcgg
     1021 atttcatgat tagaagaagc tctcgttata ttaaatataa acgatccacc tttcttgatt
     1081 ggaatttctc gttttggctt ttcatcattt gctgcaccct taatatttac cataaatttt
     1141 acattttcag gagtatgtgc agccccacga taacgaatgt ttatagtggc ggctgtatct
     1201 gtgactttct gtacgtaaac cagaaccggc gcatcactgt taactgcaat acagttgacc
     1261 catgcatcta gctgttcagg cattaaatag cttttaagtc ttttttcgct tacaacacga
     1321 gacaaatcac ctttatatag atttaattct ttttgtcgtt tcagatcaaa ttgatcataa
     1381 ctgagatcta atcctgtagt tatatcttca actaattggt tatcttgata aaatgcgctt
     1441 ccttttaact gtttcttagc ttcttcataa gtttctctgg ttattaagct taaccacgcg
     1501 tacctagacg cttcatcaaa ttcttctaat tctacatttt gtaccaaagc cttatcacat
     1561 aagggttgat cgaataaggt aaaattgtcc atttgtgctc tccaatcgaa tttaattaat
     1621 cattagggcc tatagatgat gcaataaata ttcttttttt cattaagaca atatgttcca
     1681 agaagtcacg ctatctgatg atacactttt ttatttccgt taagtttcag ttctcctccc
     1741 gttcaagtaa cattattact tatatggacg cctcccggtc tggcaagcaa tatttcgtgt
     1801 tttggtaaaa ag
//
LOCUS       DAORVT010000252         1812 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_72956_length_1812_cov_2.840637, whole genome shotgun sequence.
ACCESSION   DAORVT010000252 DAORVT010000000
VERSION     DAORVT010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72956_length_1812_cov_2.840637"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PK389_05290"
     CDS             <1..199
                     /locus_tag="PK389_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23146.1"
                     /translation="GSMFYWWGFANISEESICIGKFWGIPCIFFSALRVNFIYRARAL
                     YLLQNKEEKSYVSSTTIMTCY"
     gene            complement(196..858)
                     /locus_tag="PK389_05295"
     CDS             complement(196..858)
                     /locus_tag="PK389_05295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HQY23147.1"
                     /translation="MSFKHLTHVAMIFVTLLLTANVVGEKPVSFGMIILPAGVLLFPL
                     TYLLGDIITEVYGFKEARQIIWLGMLCNLFMAFMCRLAILAPASKAWLHQEAYTQILG
                     NGSRLMAVSTLTYFIGEFMNAYIVAKLKVKMQGKSFWLRALCGSWIGEGIETALFLPL
                     AFYGIMSNDELLKFGLFYYSFKVVYAFCTVPLANKLVNILKRREKIDHYDSVIQFNPF
                     AV"
     gene            complement(848..>1812)
                     /gene="fba"
                     /locus_tag="PK389_05300"
     CDS             complement(848..>1812)
                     /gene="fba"
                     /locus_tag="PK389_05300"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_820758.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HQY23148.1"
                     /translation="MEAANATDSPVIVQASAGARQYAGPRFLKHLMLAAVEEFPHIPI
                     CMHQDHGASPAVCQRSIQLGFTSVMMDGSLKDDMKTPSNYEYNVAITAEAVQMAHACG
                     ISVEGELGCLGSLESGLAAEEDGSGAHGKLSKEMMLTDPDEAARFVKATKIDALAIAI
                     GTSHGAYKFHQPPTGEVLVMDRVKAIHRRIPDTHLVMHGSSSVPADLIQEINEFGGKI
                     RETYGVPVSEIVEGIKHGVRKINIDTDLRLAVTGAIRRYFAKNPSEFDPRKYNHEAIE
                     AMKVICKARYEAFGTAGWASKIKPLSVEKMAERYTKGELNPRVV"
BASE COUNT          541 a          433 c          330 g          508 t
ORIGIN      
        1 gggtagcatg ttttattggt ggggttttgc aaatatatcc gaggaatcca tttgtattgg
       61 caaattttgg ggaatacctt gtatattttt tagtgcttta cgcgttaact ttatttatag
      121 ggctcgtgcc ttatatttac tgcaaaataa ggaagaaaaa tcttacgtca gttcaacaac
      181 aataatgact tgttactaaa ccgcaaaggg attaaactga atcaccgaat cataatgatc
      241 tattttttct cgacgcttta atatgttgac caatttattc gcaagcggca ccgtacaaaa
      301 cgcgtatacc actttaaagc tataataaaa taatccgaat tttagcaatt cgtcattaga
      361 cataattcca taaaatgcta atggcagaaa cagcgcggtt tcgatgcctt caccaatcca
      421 actgccacac aaggctctaa gccaaaacga cttaccctgc atttttactt tcaattttgc
      481 aacgatatag gcattcataa attcgcctat aaaatacgtc agcgtagaaa cagccattaa
      541 acgactgcca ttacctaaaa tttgtgtata cgcttcttga tgtaaccacg cctttgaagc
      601 aggcgccaga atcgccaaac gacacataaa agccataaat aaattgcaga gcatgccaag
      661 ccaaataatc tgtcgcgctt ctttaaaccc ataaacttcg gttatgatat cgccaagcaa
      721 ataggtaaga ggaaacaaca ggacgcctgc gggtaaaata atcattccaa aagataccgg
      781 tttttcgcca acaacattcg ccgttaataa taacgtaacg aagatcatcg caacatgtgt
      841 aagatgttta aacgacacgg ggatttaatt caccctttgt ataacgttcg gccatttttt
      901 cgacggagag tggcttaatt tttgaagccc aacctgccgt accaaaagcc tcataccgtg
      961 ctttgcaaat gactttcatc gcttcaatgg cttcatggtt atatttgcgt ggatcaaatt
     1021 cgctaggatt ttttgcaaaa taacgacgaa tagcccctgt aacagctaaa cgtaaatctg
     1081 tatcgatatt gatcttacgt acaccgtgct tgatcccttc tacaatctct gacacgggca
     1141 caccataagt ctctcttatt tttccaccaa attcattaat ttcttgtatc aaatcggcag
     1201 gaacgcttga cgaaccgtgc atcacaaggt gcgtatcggg aatacgacgg tgaatcgctt
     1261 taacacgatc catcaccaac acttcccctg tgggtggttg atgaaactta tatgcaccat
     1321 gacttgtgcc aatcgcaatg gccaatgcat caattttcgt tgctttgacg aaacgtgctg
     1381 cttcatctgg atcagttaac atcatctctt tgcttaactt tccatgcgcg cccgaaccat
     1441 cttcttctgc agccagtcca gattccagcg aacctaaaca ccctagttcg ccttccactg
     1501 aaatcccaca cgcatgtgcc atctgtacag cttcagccgt aatcgctaca ttatactcat
     1561 aatttgaagg ggttttcata tcatctttca gggagccatc catcatcacc gatgtaaatc
     1621 ccaattgaat cgaacgctga cagaccgcgg gcgaagcgcc atgatcttga tgcatacaaa
     1681 tcggaatatg cggaaactct tcaaccgccg ctaacataag gtgctttaaa aagcgtggac
     1741 cggcatactg ccgagcgcct gccgatgcct gcacaatgac aggactatcc gttgcattgg
     1801 ctgcttccat aa
//
LOCUS       DAORVT010000253         1801 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_73761_length_1801_cov_2.778923, whole genome shotgun sequence.
ACCESSION   DAORVT010000253 DAORVT010000000
VERSION     DAORVT010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73761_length_1801_cov_2.778923"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            370..825
                     /gene="mraZ"
                     /locus_tag="PK389_05305"
     CDS             370..825
                     /gene="mraZ"
                     /locus_tag="PK389_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005769445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HQY23149.1"
                     /translation="MFRGVNAIQVDDKGRLAIPTRYRERLQTDAKAQLILTIDTEERC
                     LLLYPLPEWETIEKKIEALPSFNRQARRIQRLLIGHATEVELDSHGRILLPPPLREYA
                     NLQKKTMLVGQGKKFEIWDEVRWHEIREEWMKEGLGNDVLPPEMESLSL"
     gene            822..1778
                     /gene="rsmH"
                     /locus_tag="PK389_05310"
     CDS             822..1778
                     /gene="rsmH"
                     /locus_tag="PK389_05310"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008291175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HQY23150.1"
                     /translation="MTISDNEKSAHIPVMLNAVVEGLNIHPEGIYIDATFGRGGHARA
                     ILEKLNQNGRLLVLDRDPEAISEANALALKNKHLSVRQGSFKNLYTWCEAEDLIGKVN
                     GILFDLGVSSPQLDAASRGFSFKNDGPLDMRMDPSTGISAADWLNSAEETVISTVLQK
                     FGEERFHRRIARAIIAARNTAPITTTRQLAEIVSKANPAWEKIKHPATRSFQAIRIFI
                     NNELEEITLGLEQALEILANGGRLAVISFHSLEDRLVKRFIQKHEKGDEHPIGLPILA
                     SQMNQRLRRLGRSIKPMDAEIAKNKRARSAILRLAEKRCHSK"
BASE COUNT          559 a          334 c          431 g          477 t
ORIGIN      
        1 tcttctacct aaagccatcc attccgaagc cactgaaata taacgtattt actgtgttaa
       61 cgtttacaac gttaagcgta ttaacgttca ggacgttagc ggttttagag caatagcaca
      121 ataaaacaac cacaaaatta tttccgaaaa atttctattt gtgatcctca gctgacatat
      181 gctataacat aaattaaact caagttcgaa ccctacttaa aatggtacta agtcactgtt
      241 tcatccatat tttgtctttg taagtttgta agtttgtaag ctttccttga caatatagtg
      301 gtcaggttac taaaatatag atatgtggga aaaaatggga taaagtgggg aaaattttgg
      361 gggcgcgttg tgtttcgagg tgtgaatgct attcaagtgg atgacaaagg ccgattggct
      421 atcccgacgc gctaccgtga acgtttacaa acggatgcaa aagcacagct gattttgacc
      481 atcgacacag aagaacgctg tctgctgctt tatccgttac ccgaatggga gaccatcgaa
      541 aagaaaatag aggccttgcc cagttttaac cgccaggcgc gtcgtattca aagattattg
      601 attgggcatg cgacagaagt ggaattggat agccatgggc gtattttgtt gccaccgccg
      661 ttgcgggaat atgcaaattt gcagaaaaaa accatgctgg tgggtcaagg aaagaaattt
      721 gagatttggg atgaagtgcg ttggcacgaa atccgtgaag agtggatgaa agaaggcttg
      781 ggaaatgatg tcttgccacc cgagatggaa tctttatcgc tatgacaata tctgataacg
      841 aaaaatctgc acacatcccg gtgatgctga acgctgtagt agagggattg aacattcacc
      901 cagaaggtat ttacattgac gccacttttg ggcgaggtgg gcacgcacgc gcaatattag
      961 aaaagttaaa tcaaaatggg cgtcttttag ttttagatcg cgatccagaa gcgatttctg
     1021 aagccaatgc gcttgctcta aagaataaac accttagcgt aagacaagga tcgttcaaaa
     1081 atctttacac atggtgtgaa gcagaagatc tgattggaaa agtaaacggt attttatttg
     1141 atctcggggt gtcttctccc caattagatg ccgctagcag ggggtttagc tttaaaaatg
     1201 atgggccttt ggatatgcga atggatccta gcacaggcat atcagccgca gactggctaa
     1261 attcggctga agaaacagtc attagtactg ttttacaaaa atttggagaa gaacgctttc
     1321 atcgacgcat tgcgcgggct attattgccg cgcgaaatac cgcgcctata acgacgacgc
     1381 gacagttagc agagattgtt tcaaaagcga atccagcttg ggaaaagata aaacacccgg
     1441 caacgcgtag ttttcaagcg attcggatat tcattaataa tgagcttgaa gagataactt
     1501 tagggttaga acaggcctta gagattttgg caaacggtgg tcgattggct gtgatcagtt
     1561 tccattcatt agaagatcgt ttggttaagc gctttattca gaaacatgaa aagggtgatg
     1621 agcatccaat tgggttgcca attctcgcga gccagatgaa tcaaaggctt cgtcgattag
     1681 gcagatcaat caagcccatg gacgctgaaa ttgcaaaaaa taaacgggca cgcagtgcca
     1741 tattaagatt agccgaaaaa aggtgtcatt caaaatgaga caagacgttg ttattaacgg
     1801 g
//
LOCUS       DAORVT010000254         1800 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_73819_length_1800_cov_3.268195, whole genome shotgun sequence.
ACCESSION   DAORVT010000254 DAORVT010000000
VERSION     DAORVT010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73819_length_1800_cov_3.268195"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..1073
                     /locus_tag="PK389_05315"
     CDS             54..1073
                     /locus_tag="PK389_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23151.1"
                     /translation="MEKLLAYGSYLEFPNHYFDHLGALPALRSRQPYNSKYALIINAT
                     LKQRKAQPHVDEKSHNEFISTIAKRTSGLLVACTLKPTSALITILAPKSSLNGLLCSL
                     AYLATSSAEIKTRFLPTSAKAGLYFYVNQAECDQHTMSCSSKASDNRTTSMFLFYISE
                     EGKTGSELKEIIKSLVENFNKKYTQILEKDPKQLQQLKSTLMHQAQGIHDCNQKKMLY
                     TACFYLQACNPNPTVAEKEEILQVCEKSAEIFLNTTQLAEWYSARTRCMWALAIIETT
                     KPEYADHLKTTSAFTSLQKLLTKVNQKLKAPVEYQILVQSEDQAITTQTVSRHPKPAA
                     SRHKR"
     gene            1152..1397
                     /locus_tag="PK389_05320"
     CDS             1152..1397
                     /locus_tag="PK389_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018402016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HQY23152.1"
                     /translation="MQQPLTQNVTLDARRLLCPLPVIRVQKAIQQLQTDDTLTVLATD
                     PGVLYDIPAWCRIHGHTVLTIEQTKQEIHIKIHVNSS"
     gene            1462..>1800
                     /locus_tag="PK389_05325"
     CDS             1462..>1800
                     /locus_tag="PK389_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23153.1"
                     /translation="MKKTLVFFSLLLIFGLFYKIALADDKIPKPIPQYAIKIISPQSE
                     ETFQNSAQSITISVVVTPELDPEDKVVALVDGNVVGEPSHSTDITLPWLARGSHTLQV
                     KVIQPKGRGAA"
BASE COUNT          652 a          386 c          294 g          468 t
ORIGIN      
        1 tgcctataat atatgcggtt ctcatgaatt gggatcaaaa accctaatat tatgtggaaa
       61 aattattagc gtatggttct tacttagaat ttcctaatca ttattttgat catttgggtg
      121 ctttgcctgc tttacgatca cgacaaccct acaactcaaa atatgcgtta ataatcaacg
      181 caaccttgaa acaaagaaag gcacaacccc atgtcgatga aaaatcacat aacgaattta
      241 tctctaccat agcgaaacga acttcagggt tactcgtagc ctgtacgtta aaaccaacta
      301 gtgcgttgat aactatttta gcacctaaaa gcagccttaa tggcttgctg tgttcgctgg
      361 cgtatcttgc cacgagcagc gcagaaataa aaacacgttt tttaccaacc agcgctaaag
      421 ccggtttata tttttatgtt aatcaagctg agtgtgatca acatacaatg tcgtgctcct
      481 ccaaagcgag cgacaataga acaacctcta tgtttctttt ttatatttct gaagaaggta
      541 aaacgggttc agaactcaaa gaaataataa aaagtctcgt cgaaaatttc aacaaaaaat
      601 atacacaaat tttagaaaaa gacccaaaac aactacaaca attaaaatca accttaatgc
      661 atcaagcaca aggaattcat gactgtaatc aaaagaaaat gttgtatact gcctgtttct
      721 atttgcaggc ttgtaaccca aatccaactg tcgcagaaaa agaagaaata cttcaagttt
      781 gcgaaaaaag cgcagaaatt ttcttaaaca ccactcaatt agcagaatgg tattcagccc
      841 gaactcgttg catgtgggca ttagcgatta ttgagacaac aaaaccagaa tatgcagatc
      901 acttaaaaac cacatctgct tttacatctc ttcaaaaact acttacaaaa gtaaatcaaa
      961 aattaaaagc acccgtcgaa taccaaatac ttgttcaaag tgaagatcaa gcaataacaa
     1021 cacaaactgt tagcagacat ccaaagccag cagcaagtcg acataaaaga tgaagaatat
     1081 tcatcaatcc ggtaaactag gcgccatgaa acaacgaaaa gccgaaaata ttaaacactg
     1141 gtatatttaa tatgcaacaa ccgctgacgc aaaatgtaac actggatgca cgaagactct
     1201 tatgtccgtt acccgtaatt cgcgtacaaa aagccataca acaattacaa accgacgata
     1261 cactcactgt tttggcaacg gatccaggcg tgctctatga tattcctgct tggtgccgta
     1321 tacatggaca caccgtccta accatagaac aaacaaaaca agaaattcac attaaaattc
     1381 atgtcaattc ttcctaaaat acctataatg aataggattc cattgttctc gtccaaactt
     1441 agagctgcgc taggaaaaac tatgaaaaaa acactggtat tttttagtct actgcttatt
     1501 ttcgggttgt tttacaagat tgcactcgca gatgataaaa taccaaaacc cattccgcaa
     1561 tatgccatta aaataatcag cccgcaatcc gaggaaactt ttcaaaattc agcccaatcg
     1621 attacgataa gtgtcgtagt tactccagaa ctggatcctg aagataaagt cgttgcctta
     1681 gtcgatggca acgttgtagg cgaaccaagt cattcaactg atattacttt accctggtta
     1741 gcaagaggca gccatactct tcaagttaaa gtcatccaac caaaaggtcg aggggctgca
//
LOCUS       DAORVT010000255         1775 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_75666_length_1775_cov_4.228488, whole genome shotgun sequence.
ACCESSION   DAORVT010000255 DAORVT010000000
VERSION     DAORVT010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75666_length_1775_cov_4.228488"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1231
                     /locus_tag="PK389_05330"
     CDS             <1..1231
                     /locus_tag="PK389_05330"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQY23154.1"
                     /translation="VLEYVELSLSKLAEKLKPSQEEVLAFYEAHQAAFTAPERVHARH
                     ILISVPKGDAALDATAKTKIASILAELKQGADFSKIAKTESNDVGSAKNGGDLGWFVR
                     GQMVPEFEKVAFELTKPGSIGGPVQTQFGYHLIQLVEHKNTEVRPLNAVKTLAEAQLK
                     REKAEALFAEKAETLVKLGFEQSTLDPIAKQLGLKKEESVPFGRDGGAGIASNPEVVK
                     VAFSETFLKEKHNSEPIKMSDDATVIIRVKDHQPAVQQTLVQAHAKIADLLIVEGARE
                     RAKSIGEGILKKIQNGAEKPFDLARQQELNWTLKSKVIRSAADPNRDIVVAAFQIPEN
                     TPPEKPNLNGFSLPNGDYLVLSLNKVADGDWSTLSSDVQASYRQGLTEFSSQLEYALY
                     VSHVSQAAKVKLFKDPL"
     gene            1237..>1775
                     /locus_tag="PK389_05335"
     CDS             1237..>1775
                     /locus_tag="PK389_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQY23155.1"
                     /translation="MNTNIIELKGVSKYYHDHPVLQDIHLEVKNREFLTILGPSGCGK
                     TTLLRLISGFETPSNGSILMNGKDVSNLPPHLRHVNTVFQSYALFPHLNVFDNVAFGL
                     RCKHLPKEEIVHRVTEALRMVKLIDLATRKPHQLSGGQQQRIAIARAVVNQPMVLLLD
                     EPLSSLDYRLRKTMQMELK"
BASE COUNT          552 a          318 c          402 g          503 t
ORIGIN      
        1 tgttttagaa tacgttgaac tctctttatc aaaattagct gaaaagctta aaccttctca
       61 agaagaagtg ttagcttttt atgaggcaca ccaagcggct tttactgcac ctgaacgtgt
      121 gcatgccaga catatattaa tttcggtgcc gaaaggagac gctgccttag atgcaacagc
      181 caagactaaa attgcttcaa ttttagcaga attaaagcaa ggcgctgatt tttctaaaat
      241 agcgaaaacg gaatcgaatg atgtgggatc agccaaaaat ggtggggatt tgggttggtt
      301 tgtgcgtggg caaatggtgc cagaatttga aaaagttgct tttgaattaa caaaacctgg
      361 cagtattggc ggcccagtgc agacacagtt tggataccac ttgatccaat tggttgaaca
      421 taagaacaca gaagtgcgac ccttgaatgc ggtaaaaacg ttggcggaag cacagctgaa
      481 acgcgaaaaa gcagaagcct tgtttgcaga aaaagcagaa acattggtca aattgggatt
      541 tgaacaatcc actttagatc cgattgcgaa acaattgggt ttaaaaaaag aagagagtgt
      601 tccttttggt cgtgatgggg gagctggtat tgccagcaat ccagaagtcg ttaaagtggc
      661 ttttagtgaa acgtttttaa aagaaaaaca taacagtgaa ccgattaaaa tgtctgatga
      721 tgcaactgtc attattcgtg tgaaagatca tcagccggcg gttcaacaaa cactggttca
      781 ggctcatgcg aagattgcgg atctgttaat cgttgaaggt gcaagagaac gggcaaaatc
      841 gattggtgaa ggcattttaa aaaaaatcca aaacggggct gaaaaacctt ttgatctagc
      901 gcggcaacaa gaactcaatt ggacgcttaa atcgaaagtt attcgctctg cggcagatcc
      961 gaatcgcgac attgttgtcg ccgcttttca aatacccgag aatacaccgc ccgaaaaacc
     1021 gaatctgaac ggattttctt taccgaatgg agattactta gttttgagtt taaataaagt
     1081 tgccgatggc gactggtcta cgttgtcttc agatgtacag gcttcgtata ggcaaggtct
     1141 tacagaattt tcaagtcaat tggaatatgc actgtatgta agtcatgtat cacaagcggc
     1201 gaaagttaag ttatttaaag atcctttata accgccttga atacaaatat tattgaatta
     1261 aagggtgtct caaaatatta tcatgatcat cctgtattgc aggatattca tctagaagta
     1321 aaaaatcgcg aatttttaac gattttgggg ccatcagggt gtggtaaaac cacattattg
     1381 cgccttattt ctggttttga aacccccagt aatgggtcca ttttaatgaa tggaaaagat
     1441 gtttcaaatc ttccgccaca tcttcgtcat gttaataccg tttttcagag ttacgcgctt
     1501 tttccacatt tgaatgtttt tgataatgtt gcctttggtt tacgttgtaa acatttacct
     1561 aaggaagaga tcgtgcatcg ggtaacagaa gccttgcgga tggtaaagtt gattgattta
     1621 gcaacgcgta aaccccatca attaagtggt ggtcaacagc aacgtattgc catcgcgcgt
     1681 gcggttgtta accaaccgat ggtgttacta ctcgatgagc ctttaagttc tttagattac
     1741 cgtttgcgaa aaacgatgca aatggaatta aagca
//
LOCUS       DAORVT010000256         1744 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_78154_length_1744_cov_3.645944, whole genome shotgun sequence.
ACCESSION   DAORVT010000256 DAORVT010000000
VERSION     DAORVT010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78154_length_1744_cov_3.645944"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1743)
                     /locus_tag="PK389_05340"
     CDS             complement(<1..1743)
                     /locus_tag="PK389_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQY23156.1"
                     /translation="MFSEQESQAVFFLKKENVSRLDVINYISHGTFHQMEEPNEPDMS
                     QNQEEDLSQESNVRSPLESYAVNLNNRAKMGKIDPLVGREDEIQRTIQILCRRRKNNP
                     LFVGEAGVGKTAIVEGLAKLVVEGKVPDAIKNSTIYSLDLGSLLAGTKYRGDFEKRFK
                     ALLQQLKREAGAILFIDEIHTIIGAGAASGGVMDASNLLKPLLASGELKCVGSTTYYE
                     YRGIFEKDMALSRRFQKVDVKEPTVDETISILKGLKERFEQHHNVRYSFAALRAAATM
                     AIRYIPDRFLPDKAIDVVDEAGAYQRLLPVNKRKKVIGVSDIERIVAKIARVPEKSVS
                     VSDKNALRNLERDLKLVIFGQDEAVASLSDAIKMGRSGLSDPDRPVGCFLFAGPTGVG
                     KTEVTRKLSDILGIELLRFDMSEYMESHTVSRLIGAPPGYVGFEQGGLLTDAVNKHPH
                     SVLLLDEIEKAHPDVYNLLLQVMDHGTLTDSNGRKTDFRHVILVMTTNAGAEDMNRSS
                     MGFTEQDHTTDALSVIKKKFTPEFRNRLDAIIQFKSLNRIAIDLIVEKFLTELQARLE
                     EKHVTLEVDKPAREW"
BASE COUNT          497 a          408 c          290 g          549 t
ORIGIN      
        1 ccactctcgc gctggtttat ccacttctaa agtcacatgc ttttcttcta atcttgcttg
       61 caattcggtt aggaattttt ctacgattaa atcaattgca atgcgattta aagatttgaa
      121 ttgaataatc gcatcgagcc gattacgaaa ttccggtgta aatttctttt taataacact
      181 caaggcatcc gtagtgtgat cttgctcggt aaaacccatc gaacttctat tcatatcttc
      241 agcgcctgca ttggtcgtca tcaccaatat cacatgtcgg aaatcggttt ttcgtccatt
      301 agaatctgtt aacgtgccat gatccatcac ttgcaataac aaattgtaaa catccggatg
      361 tgctttttct atttcatcta ataagagtac actatgagga tgcttattga cggcatcggt
      421 taataagcct ccttgttcga atccgacata ccccggtggt gcaccaatta aacgcgaaac
      481 agtatggctt tccatatatt cagacatatc aaaccgtaat aattctatac ctaaaatatc
      541 tgacaacttc cgagtgactt ccgttttacc tacccctgtt ggtcccgcaa atagaaaaca
      601 acccacaggt ctatctggat cggacaatcc agaacgaccc attttaatcg catcggataa
      661 actggcaacg gcttcgtcct gaccaaagat aaccaatttt aagtcgcgct ctaaattacg
      721 taaggcattt ttatcggaaa cagaaacaga tttttctggt acacgtgcaa tttttgcaac
      781 aatccgttct atatcagaaa ccccaatcac ctttttgcgt ttattgactg gcaatagtcg
      841 ttgatacgct cctgcttcgt ccaccacatc aattgctttg tcaggcaaaa agcgatcggg
      901 aatatagcga atcgccatag tggccgcggc acgcagtgct gcgaatgaat agcgaacatt
      961 atgatgttgt tcaaaacgtt cttttaatcc ttttaaaata ctaatcgtct cgtctacggt
     1021 tggctctttc acatccactt tttgaaaacg tctagacaag gccatgtctt tttcgaatat
     1081 cccacgatac tcgtaatatg tagtggaacc tacgcattta agctcacccg acgccaataa
     1141 aggcttcagc aaattagagg catccataac accaccggat gcagcacctg caccaataat
     1201 ggtgtgaatt tcatcgataa ataagattgc tcccgcctcg cgctttaatt gttgcaacaa
     1261 tgccttaaat cttttttcaa aatcacctct atattttgtg cctgctaaca gtgatcctaa
     1321 atccaatgaa taaatggtgc tatttttaat cgcatctggc actttgcctt ccaccactaa
     1381 ttttgccaat ccttctacaa tggctgtttt tccaacaccc gcttctccaa caaacaaagg
     1441 attgtttttt cgacgtctgc ataatatttg aattgttctt tgaatttcat cttcacgacc
     1501 aaccaaagga tcaatcttac ccattttagc tcgattattt aaattaacgg catagctttc
     1561 gagtgggctt cgcacatttg attcttgaga caaatcctct tcttggttct gagacatatc
     1621 gggttcgttg ggctcttcca tttgatgaaa agtaccgtga gaaatataat taatcacatc
     1681 taatcgagaa acattttcct tttttagaaa aaagacggct tggctttctt gctcactaaa
     1741 aata
//
LOCUS       DAORVT010000257         1722 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_79940_length_1722_cov_3.364727, whole genome shotgun sequence.
ACCESSION   DAORVT010000257 DAORVT010000000
VERSION     DAORVT010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79940_length_1722_cov_3.364727"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..>1722)
                     /locus_tag="PK389_05345"
     CDS             complement(193..>1722)
                     /locus_tag="PK389_05345"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQY23157.1"
                     /translation="HANKIKSEFLADMSHEIRTPLNGVVGFVNLLQKTELNPKQQEYI
                     RTIQKSANNLLAILNDILDFSKMEAGKLRIERSIMDVRECIDEILNLLLPHAQEKNIA
                     LIPLIYSDVPAQVLGDPLRIKQIVTNLVSNAIKFTERGSVIVRVMLENESFPHTTLRV
                     NVTDTGIGLSPEEQKVLFHAFNQTKSAHKSGGTGLGLVISKKLVEQMGGTIGVESEPQ
                     QGSTFWFTFQVESTPQVEFTAQVEPTLETQPALTSSTLSSFVPSLDVLEPPVNVLAVD
                     DNPENLKLIRILLEDMGIEVTTTDNGKAAIVAVRQKTFDLILLDIRMPNMTGLEAAHI
                     IRNVEAEYHRKPTPIIALTANALAAEKESLFKNDINDYLAKPIGEIELKNILKKWIQS
                     ETNVCTIDWALGKQLAGGRVELAQEFFEKLREILPLEKQRINDDYQKRNWQAFSDRIH
                     KLHGACCYCGVPELKSCVQTLEAAAVSCFPDIIKPHLEAFNLAVDAVLTATENTTIHD
                     T"
BASE COUNT          475 a          319 c          360 g          568 t
ORIGIN      
        1 actagtgagt caataacatg gactcttttt taggaaccca atgcgaatga aaaacagcat
       61 ttatttgatt attattgtgg ctcttaacgt gggcataaaa aaaagcccta atagaggtta
      121 ttagggctaa aaaaatcatg attaataatg tggaaaacaa tacatctata ataataggcc
      181 aaattttgtt tgtcaagtat cgtgtattgt cgtattttct gtggccgtta ataccgcgtc
      241 tacagcgagg ttaaaggctt ccaaatgtgg tttaataata tccggaaaac atgagactgc
      301 agctgcttct aatgtttgca cgcaactttt taattcaggc acaccacaat aacaacaagc
      361 accgtgtagt ttatgaatac gatcactgaa ggcttgccaa tttctttttt ggtaatcgtc
      421 attaattcgt tgtttctcca gaggcaaaat ttctcgcaat ttttcaaaaa actcttgtgc
      481 gagctctacc ctgccgcccg ctaattgttt gcccagtgcc caatcaattg tgcagacatt
      541 ggtttcggat tgtatccatt ttttgaggat atttttaagt tctatttctc caatgggttt
      601 tgctaggtaa tcattaatgt catttttgaa taaggattct ttctcagcgg ccagtgcgtt
      661 ggcggttaat gcaataatgg gtgtgggctt tcgatggtat tctgcttcta cgtttcgaat
      721 aatgtgcgca gcttcaaggc ccgtcatgtt tggcattcgg atatccaata atattaaatc
      781 gaaggttttc tggcgcactg ctacgatggc cgcttttcca ttatcggttg tagttacctc
      841 aatgcccatg tcttctaata aaatgcggat cagttttaag ttttctggat tatcatccac
      901 agccaaaacg ttaacaggag gttctaaaac atcgagagaa ggaacaaatg aagacaatgt
      961 tgatgatgtt aaggctggtt gtgtttctag cgtaggttca acttgcgcgg tgaattcaac
     1021 ttggggtgtg gattcaacct gaaaggtaaa ccaaaatgta gagccttgct gtggttcgct
     1081 ctcaacacca atggtaccac ccatttgttc gactaatttt ttagaaatca ctaatccaag
     1141 acccgttcca cctgatttat gagcgctttt tgtttgatta aaagcatgga ataatacttt
     1201 ttgttcttcg ggtgataaac caatacccgt atcggtcaca tttacacgaa gggttgtatg
     1261 tggaaaggat tcgttttcta acatgacgcg aacaatgacg ctgccgcgct cggtaaattt
     1321 aatagcattg ctgaccaaat ttgtcacaat ttgtttgatt ctgagcggat cacccaaaac
     1381 ctgtgcgggc acatcggagt aaataagggg gatcagagca atgttctttt cttgtgcgtg
     1441 tgggagcagc agatttaata tttcatctat gcattctcgg acgtccataa tagatcgttc
     1501 tatgcgtaat ttcccggctt ccatttttga gaaatcaaga atatcgttta ggattgccag
     1561 taagttatta gcagattttt gtatggtgcg aatatattct tgttgctttg ggttgagttc
     1621 agttttttgt aataaattaa caaatccaac cacgccattc agtggggtac gaatttcatg
     1681 gctcatatcg gctaaaaatt cggatttaat cttattggca tg
//
LOCUS       DAORVT010000258         1717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_80412_length_1717_cov_2.336943, whole genome shotgun sequence.
ACCESSION   DAORVT010000258 DAORVT010000000
VERSION     DAORVT010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80412_length_1717_cov_2.336943"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="PK389_05350"
     CDS             complement(<1..828)
                     /locus_tag="PK389_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004675686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY23158.1"
                     /translation="MNIQNDAFAALRYRDFSIVTLNQFCLTLAILIQEIIVAYSLYQI
                     TRDPLTLGFIGLAEAIPFITLSLWGGYFADKLNKQTIMKVCLFFAIPLPLVLWALFHL
                     HGLEKIGINTLSWGIYAVIFALGTIRGFYNPSATSLKPFLIPRELYANGATWTTIGWQ
                     SGVIIGPMLGGFMLAFLGRETSLLTVTALLAICFILISLLQKRTFPKMETENVLQSLT
                     DGFRFIWKTKIVLWAISLDLVSVLFGGVIALLPIFAEEILKVGPEGLGYLRAAPSIGA
                     "
     gene            1065..1259
                     /gene="rpmI"
                     /locus_tag="PK389_05355"
     CDS             1065..1259
                     /gene="rpmI"
                     /locus_tag="PK389_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HQY23159.1"
                     /translation="MPKMKTRRGAAKRFKATANGIKRKQAFKRHILTKKSAKRIRQLR
                     GCVMVHVSDVASVRRMCPYI"
     gene            1271..1630
                     /gene="rplT"
                     /locus_tag="PK389_05360"
     CDS             1271..1630
                     /gene="rplT"
                     /locus_tag="PK389_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HQY23160.1"
                     /translation="MARVKRGVQAHRRHKKILARAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQKKRQFRALWIARINAGARLNGLSYSRMIDGLKKAQVIIDRRVLADIAMHD
                     AVAFTALAEKAKGALAA"
BASE COUNT          564 a          338 c          350 g          465 t
ORIGIN      
        1 cgcgccaatc gaaggagcag cacgtaaata gccaagacct tcaggaccga cttttaaaat
       61 ttcctcggca aaaatcggga gtagggcgat cacaccacca aataaaacag aaacaaggtc
      121 gagtgaaatc gcccacagca caattttagt tttccaaata aagcggaaac catcggtcaa
      181 actttgcagc acattttctg tttccatctt tgggaaagtg cgcttttgca acagactaat
      241 caaaatgaag cagatggcta ataaggcagt aacggttaat aaactagttt ctcgaccaag
      301 aaatgccaac ataaaaccac caagcatagg gccaataatc accccacttt gccagccgat
      361 ggttgtccac gttgcaccat tggcataaag ttcacgtgga attaaaaatg gcttgagtga
      421 tgtagcagaa gggttgtaga aaccacgaat ggtgcctaaa gcaaaaatca ctgcataaat
      481 accccaagaa agcgtattaa tgccaatttt ttctaaacca tgtaaatgaa acaaagccca
      541 aagcactaaa ggtaaaggaa tcgcaaagaa taaacacact ttcatgatgg tctgtttatt
      601 caacttatct gcaaaatatc caccccataa cgatagtgta ataaacggaa tcgcttcagc
      661 caatccaata aaacccaagg tgagtggatc gcgagtaatt tggtagagcg aataagcaac
      721 aataatttct tgaatcaaaa tcgcaagcgt taagcaaaat tgattcaacg taacgatcga
      781 gaaatctcga tagcgtaacg ctgcaaatgc atcattttgt atattcatat tgattgcaag
      841 ttaaagattc aaagctatta tagaggctaa ttttatagat ttttctcata atttgagaat
      901 taattaagag aaataagact tagagcaaat cgaatattta gtgtcaaaaa tgagcatttt
      961 acttttgttt taagtgagga tgctttagaa tgtcggctcc cttacctgca ggtcgttttt
     1021 gcattggtgc aaacgttccg aacaggtgtt caaaagaggt tgttatgcct aagatgaaaa
     1081 ctcgccgtgg tgcagctaaa cgctttaaag cgactgctaa cggtatcaag cgtaaacaag
     1141 cgtttaaacg ccacattttg accaaaaaat ctgctaagcg tattcgtcaa ttacgcggct
     1201 gtgtaatggt tcacgtaagt gacgttgctt cagttcgtcg tatgtgccca tacatctaag
     1261 gagattataa atggctcgtg taaaacgtgg tgtacaggcg catcgtcgtc ataaaaaaat
     1321 tcttgctcgc gctaaaggtt actatggtgc tcgttcacgc gtttatcgcg tagcgttcca
     1381 agcagtaatc aaagctggtc aatatgcata ccgtgaccgc cgtcaaaaga aacgtcaatt
     1441 ccgtgcttta tggattgcgc gtatcaatgc tggtgctcgt ttaaacggtt tgtcttacag
     1501 ccgtatgatt gatggcttga aaaaagctca agtgatcatc gaccgtcgtg tattggctga
     1561 catcgctatg catgatgcag ttgcatttac ggctttagct gaaaaagcta aaggtgcatt
     1621 agctgcataa gcttagagat tcaaaaaaag accgcttttt agcggtcttt tttatcatct
     1681 aaagttttat tttttcgacc aattagcaga aaaataa
//
LOCUS       DAORVT010000259         1714 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_80655_length_1714_cov_2.654008, whole genome shotgun sequence.
ACCESSION   DAORVT010000259 DAORVT010000000
VERSION     DAORVT010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80655_length_1714_cov_2.654008"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PK389_05365"
     CDS             <1..420
                     /locus_tag="PK389_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23161.1"
                     /translation="GISTEVIDGTLKIWITPEVQKGSLPMPRLFRFSAKTLTSTEIPI
                     HLSTAPGIIIANKQEMLSIPAIKDLKLDTQEISSDGYKAEISSPNTGSVANLFLLNTK
                     RALLISKNGHVINVSDEKNKNSHKYIKFLGWIRTQSQ"
     gene            430..1371
                     /locus_tag="PK389_05370"
     CDS             430..1371
                     /locus_tag="PK389_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23162.1"
                     /translation="MPHSKDKALKEINRLARQHNLTIADITKVLQTQSIQSARTETLM
                     KLFAYLGGIFIFSGLSIYIGTFWSTMNVAMKITLTFGTGIVLYLLALILPVYQKKYET
                     MLTPLFLTATLFQTIGLFVAVNEFRDGLDFKISSVLIFGVMLIQQLVTFKKIKRASLI
                     FTALFFWCAFLATTLDLVHASDKLIGIVVGLSVLFIAYMLDKAFYKAVASFWYFVGSI
                     LFLNSLFDTLRSTYFEVIFFGISGLMLYLSTLIRNRTLLFVSTLSALGYIGYFTSKHF
                     MHSAAWPILLVILGLVCFGVGVMVLKICKKYMIEPMS"
     gene            complement(1409..>1714)
                     /locus_tag="PK389_05375"
     CDS             complement(1409..>1714)
                     /locus_tag="PK389_05375"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HQY23163.1"
                     /translation="AMALHAGLRYPEPLAGIIALSSYLALPDTFVKERDLANQNTPIF
                     MAHGLFDTVVPIMLDQMSKEQLEDLGYQIDWFTYPMAHTVVPEEIDDIYHFLERIIK"
BASE COUNT          509 a          360 c          295 g          550 t
ORIGIN      
        1 ggtatcagca cagaagttat agacggaacc ttaaaaatct ggataacccc agaagtccaa
       61 aaaggcagtc tacctatgcc acgattattc cggtttagcg ctaaaacact cacttcgaca
      121 gaaattccaa tccacttatc aactgctccc ggaatcatta ttgcgaataa acaagagatg
      181 ctcagcatcc ctgcaattaa agatttaaag ctcgataccc aagaaatttc ttccgatggc
      241 tataaagcag aaatctcgtc tcccaataca ggcagcgttg ctaatctttt tttattgaat
      301 acaaaaagag cactactgat atcaaaaaat ggtcatgtta ttaatgtttc tgatgaaaaa
      361 aacaagaata gccataaata cattaaattt ctaggatgga tacgcacaca atcacaataa
      421 ggaattttta tgcctcactc taaagataag gccctaaaag aaattaatcg tcttgcacgc
      481 cagcacaact tgacaatcgc agacattaca aaagtcttac agacacaatc tatacaaagc
      541 gctcgcacag aaacgttaat gaaacttttc gcttatctgg gcggcatttt tattttttct
      601 ggcttaagta tttacattgg cacattttgg tcgaccatga atgttgcgat gaaaattacc
      661 cttacctttg gcacgggtat tgtactctat ctgttagcgc tgattttacc tgtgtatcaa
      721 aaaaaatacg aaaccatgtt aacgcccctt tttctaaccg ccacattatt tcagaccatt
      781 ggtcttttcg ttgccgttaa tgaatttaga gatggattgg attttaaaat ttcttctgta
      841 ctcatttttg gcgttatgct tatccaacaa cttgttacct ttaaaaaaat taaacgtgct
      901 tctttaattt ttactgctct ttttttctgg tgcgcatttt tagcaaccac attagattta
      961 gtacatgcaa gcgacaaact gatcggcatc gttgtcgggc tatccgtact ctttattgct
     1021 tacatgttag ataaagcctt ttacaaagcc gtcgccagct tttggtactt tgtaggctcc
     1081 attctgtttt taaatagtct gtttgatacg cttcgcagca cgtattttga agtaattttc
     1141 tttgggatca gcgggcttat gctttacctc agcactttaa tacgtaatag aacactgctt
     1201 tttgtgagta cattatctgc attaggatac attggctatt ttaccagcaa acactttatg
     1261 cattcggccg cttggcctat tcttttggtc atattaggat tggtgtgttt tggcgtaggc
     1321 gttatggttt taaaaatttg caaaaaatat atgattgaac cgatgtctta accctcaccc
     1381 gatgctgcac accacctcat ttacctacct attttattat ccgctctaaa aaatgataga
     1441 tatcatcgat ttcttctgga accactgtat gtgccattgg gtaggtaaac caatcgatct
     1501 gataacccaa gtcttctaac tgttctttag acatttgatc aagcatgata ggcactacag
     1561 tatcaaataa accgtgcgcc ataaaaatag gcgtattttg attcgcaaga tcccgctctt
     1621 ttacaaatgt atcgggcaag gctaaataac tggataatgc aatgatgcct gctaacggct
     1681 ctggataacg tagtcctgcg tgcaatgcca tcgc
//
LOCUS       DAORVT010000260         1698 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_81998_length_1698_cov_3.601339, whole genome shotgun sequence.
ACCESSION   DAORVT010000260 DAORVT010000000
VERSION     DAORVT010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81998_length_1698_cov_3.601339"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PK389_05380"
     CDS             <1..526
                     /locus_tag="PK389_05380"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HQY23164.1"
                     /translation="SLSEKSSTEITDPEVLKNFRYTVHETIRKVTDDMGRRQTFNTAI
                     AAMMELLNAIQGFSAAKHYIKEALESLLLMLSPIAPHITHTLWHTMGNADAIVDHAWP
                     TFDPHALIKDTLEFALQVNGKLRGSLTAPASTDNKALETLALAHENIKRHVEGKIIQK
                     IIVIPKKLINIVVK"
     gene            528..1040
                     /gene="lptE"
                     /locus_tag="PK389_05385"
     CDS             528..1040
                     /gene="lptE"
                     /locus_tag="PK389_05385"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HQY23165.1"
                     /translation="MFRRSRLKIFHFIIVLSLLTGCGFHLRGAFSISPSLQTLQILPN
                     QSYDVFQRTLKQFLKSNNIKIVEQNTENTIPVTTLTILNQAFSEHAVAYSSNGLPNRA
                     ALELKISYQLTDEKGKPLGNNTVVIVERELMLNPNAVLGTDNERSHLQADLYRDAASQ
                     LVRQLSMISE"
     gene            1037..>1698
                     /gene="holA"
                     /locus_tag="PK389_05390"
     CDS             1037..>1698
                     /gene="holA"
                     /locus_tag="PK389_05390"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HQY23166.1"
                     /translation="MRILAQDLPQHLKHGLANCYFVFGDEPLQQSESIDAIRLAAKQQ
                     GYTEREVFQLTSNFEWASLFSSVDTPSLFSEKRLIECCLNDGKIGKGTADTLSKLATL
                     SHSTSILFLFSASKLDANIQKSKGFLALEQRGITICARSLSLKETFVWIEKRLATAGF
                     QTTPLVTQELLERTEGNLLATSQVITKLQLCVDPQQTPVLSIEDIRTIIDTDTRFTIY
                     DLV"
BASE COUNT          545 a          378 c          301 g          474 t
ORIGIN      
        1 atcactgtca gaaaaatcat caacggaaat aaccgatcca gaagtgctca aaaattttag
       61 atacactgtg cacgaaacga ttcgaaaagt aaccgatgat atggggcgtc gccaaacttt
      121 taataccgcc attgcggcta tgatggaatt attaaatgcc atacaaggct tttctgctgc
      181 aaaacactac attaaagaag ctctagaatc attactctta atgctgtctc ccattgctcc
      241 ccatattacc catacactgt ggcacacaat gggcaatgca gatgctattg ttgatcatgc
      301 ttggccaact tttgatcccc acgcgctcat caaagacact ctagaatttg cgctacaagt
      361 gaatggaaaa cttcgcggat cactcactgc acctgcatcc actgataata aagcattaga
      421 aacgcttgca ttagcacatg aaaacattaa gcgtcatgtc gaaggaaaaa ttatccaaaa
      481 gattattgtg atccctaaaa aactgattaa cattgtggta aaataatatg tttaggagat
      541 ctcgcttaaa aatattccac tttataatag tactttcgtt actgacaggc tgtgggtttc
      601 atttaagagg cgctttttct atatcaccat ccttacaaac acttcaaatt ttaccgaacc
      661 aatcctatga cgtttttcaa cgtacactaa aacagtttct aaaaagcaat aatattaaaa
      721 tagtcgaaca aaatactgag aataccatac cagtcacgac actcactatt ttgaatcaag
      781 cgttttctga acacgcagtt gcctatagct caaatggtct accaaataga gcagcgctcg
      841 aactcaaaat tagctaccag cttaccgatg agaaaggcaa gcctcttgga aacaataccg
      901 tggttatagt cgaacgcgaa ctcatgctga accctaatgc tgttttagga accgacaatg
      961 aacgctctca ccttcaagca gatctctatc gtgatgcggc ttcacaacta gtgcgacagt
     1021 tatcgatgat ttctgaatga ggatccttgc tcaagattta cctcagcatt taaaacacgg
     1081 tttagccaat tgttattttg tctttggcga tgagccacta cagcagtccg aatctataga
     1141 tgccattcgt ttagcggcaa aacaacaagg ttatacagaa cgtgaggttt ttcaactaac
     1201 atcaaatttt gagtgggcat ccttattttc gagtgttgac acgcccagct tgttctcgga
     1261 aaaacgctta atcgaatgtt gtttgaacga cggaaaaata ggcaagggta ctgcggatac
     1321 actttctaaa ctggcaacct tgtcgcattc tacatcaatc ctttttctat tctctgcttc
     1381 aaagcttgac gctaacatcc aaaaatccaa agggttttta gcattggaac aaagaggcat
     1441 taccatttgc gcacgttctc tctcgcttaa agaaacattc gtgtggattg aaaaaagact
     1501 ggctactgct ggttttcaaa caacgcctct ggtcacacaa gaattattgg aacgaacaga
     1561 aggcaattta ctggccacca gtcaggtcat cacaaaactg caattgtgtg tagatccaca
     1621 acaaactccc gtattatcta ttgaagatat tcgcactata atagacaccg atacccgttt
     1681 tacgatatat gatcttgt
//
LOCUS       DAORVT010000261         1682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_83414_length_1682_cov_2.361401, whole genome shotgun sequence.
ACCESSION   DAORVT010000261 DAORVT010000000
VERSION     DAORVT010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83414_length_1682_cov_2.361401"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PK389_05395"
     CDS             complement(<1..488)
                     /locus_tag="PK389_05395"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HQY23167.1"
                     /translation="MKPKVKKRCAWVTDAPIYISYHDLEWGLPIYDDRLLFEFLVLEG
                     MQAGLSWLTILKKRDNYRKSFDNFNAEKIVKYDQQKINELLMNEGIIRNKLKIASVIT
                     NAQSFLRVTQEFSSFSEYIWGFVNGRPIQNRWQNITEVPSTSVISDQLSKDLKKRGFK
                     FVG"
     gene            complement(513..589)
                     /locus_tag="PK389_05400"
     tRNA            complement(513..589)
                     /locus_tag="PK389_05400"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(553..555),aa:Arg,seq:tcg)
     gene            complement(655..1587)
                     /locus_tag="PK389_05405"
     CDS             complement(655..1587)
                     /locus_tag="PK389_05405"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006034901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HQY23168.1"
                     /translation="MVDEQNILQTVLLEHIREQRRARRWSMFFKLIFLGFLIYLFTSF
                     YNRDLPQPISLGQPHTAVIDVYGEIAAEDPANADDIRDALRTAFTTKQVKGVILRINS
                     PGGSPVQARQIFSEIRNLKKQHPHIKIYAAIEDLGTSAAYLIAAAVDNIYADKTSIVG
                     SIGVKIDSFGYVEGMKKIGVERRLYTAGKYKGILDPFSPRNPEEEGFINEQLRTVHMA
                     FIENVREGRGNRLRETPDIFSGLFWSGEQALTLGLIDGFGDAQFIAKELIKIENLVDY
                     TPNPTLLDKLARRMGASIGQTLLLNAGLIQKGAR"
BASE COUNT          481 a          366 c          324 g          511 t
ORIGIN      
        1 ccgacaaact tgaaaccacg cttttttaag tcttttgata gctgatctga aatgacactg
       61 gtgctgggta cttcggtgat gttttgccat cgattttgta tagggcgacc attaacaaat
      121 ccccaaatgt actctgaaaa actagaaaat tcttgggtga cgcgtaaaaa agattgtgca
      181 tttgtaatca cggatgcgat ctttaatttg tttcgaataa tgccttcatt catcagcaat
      241 tcattaatct tctgctgatc atatttcaca attttttcgg cattaaaatt atcaaaactt
      301 tttcggtagt tatcgcgttt ttttaaaatg gttagccagc ttaatccagc ttgcattccc
      361 tctaatacga gaaattcaaa taataaacga tcgtcataaa taggaagacc ccattctaag
      421 tcgtgatagc taatataaat aggcgcatca gtaacccaag cgcagcgttt tttaacttta
      481 ggtttcatcg ttcgtatatc tgttagaaat tatggtgcgc ccggagagat tcgaactccc
      541 gacaccttgg ttcgaagcca agtactctat ccaactgagc tacgggcgca cagacctgag
      601 gatattctca cagaagagca atggggtcaa tggaaaaccc cattgctcga tcgcttaacg
      661 cgcccctttc tgtatcaagc ctgcattcaa taacaaagtt tgtccaattg aagcgcccat
      721 gcgtcgggcc agcttatcca acaaggtagg gttaggcgtg taatcaacta aattttcaat
      781 tttaataagt tctttggcaa taaattgagc gtctccaaag ccatcgatta aacccagggt
      841 tagcgcctgt tcaccgctcc aaaacaatcc cgaaaagata tcaggggttt cacgtaatcg
      901 attaccacga ccttcgcgta cattttcaat aaaggccatg tgaactgttc ttaattgttc
      961 gttgataaag ccttcttctt ctgggttacg cggcgagaac ggatctaaaa tacctttgta
     1021 tttgcctgcc gtatataggc gccgttccac accaattttt ttcatgccct cgacataacc
     1081 aaaagaatca attttgacgc cgatagatcc cactatactg gttttgtcgg catagatgtt
     1141 gtccactgcc gcagcaatta aataggctgc agaagtacct aaatcttcaa tagccgcata
     1201 aattttaata tgggggtgct gtttttttaa attgcgaatt tcagagaaaa tctgtcgagc
     1261 ttgtacaggg ctaccacctg ggctattaat ccttagaata acacctttga cttgcttcgt
     1321 tgtaaaagcc gttcttaagg catcccgtat gtcatccgca ttcgcaggat cttctgcggc
     1381 aatttcacca tagacatcaa taactgctgt atgtggctgc ccaagactta tgggctgcgg
     1441 taaatcgcga ttataaaaag atgtaaataa ataaatcagg aacccaagga agattaattt
     1501 aaaaaacata ctccaacgtc ttgcgcgacg ttgttctcta atatgctcta ataaaacggt
     1561 ttgtaagatg ttttgttcat caaccatgtt gcatttcctc cgtcagctta tctaggctgg
     1621 cttgcagttg tttatctaaa ggcgcttcaa ttgaaagtgg attttcatgc cccggcaaag
     1681 tt
//
LOCUS       DAORVT010000262         1678 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_83744_length_1678_cov_2.685767, whole genome shotgun sequence.
ACCESSION   DAORVT010000262 DAORVT010000000
VERSION     DAORVT010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83744_length_1678_cov_2.685767"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PK389_05410"
     CDS             complement(<1..427)
                     /locus_tag="PK389_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase A"
                     /protein_id="HQY23169.1"
                     /translation="MSKKIRVGVLFGGRSAEHEISLLSAKTIVEAMDKEKYEIVLIGI
                     NKSGEWFLHSDLTCLSNASDPKKIHFKKENEKENVALVAKEMDNPLVSLTGKALNCPL
                     DVIFPALHGPYGEDGTVQGLLKLANIPFVGAGVLASAIGM"
     gene            complement(536..751)
                     /locus_tag="PK389_05415"
     CDS             complement(536..751)
                     /locus_tag="PK389_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23170.1"
                     /translation="MVMVNKTKVFVGLFIGLALLTTGCTPGNHFTQEPVEGTSIDILA
                     VSEAGSTGAGARSISSPSPAPPPVPPP"
     gene            complement(801..>1678)
                     /locus_tag="PK389_05420"
     CDS             complement(801..>1678)
                     /locus_tag="PK389_05420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HQY23171.1"
                     /translation="YKFIQKKTRPTSTHDLKDYESEPIIFGTFQRQKTDVSANINGYH
                     IKSGGVGYLLDYNFKTALDLFEIGGGATYAESHTNIATSDTLNSDYGQILGYANLVEN
                     GYFLGLFLSGTESWSKSKRAISGTTLQALSRFKGAAFFSKVRIGHMSRQSDWQLIPEA
                     SLYYVHFHESGRNETGADISNLSVSSSAADGVKAGIGGRVMYIQPAVTTEIRAFYLRD
                     LKNPLLNPSFTFSASGAPFASSAQNNKKNNLNLGASLKYFVRPDLSITGVYDFLVKQQ
                     GYTENNASLLMCWNF"
BASE COUNT          498 a          365 c          311 g          504 t
ORIGIN      
        1 ccataccaat cgcggaagcg agtacccctg cgcccacaaa tggaatgttg gccaatttta
       61 ataatccttg cacagtgcca tcttcgccat atggcccatg caatgcagga aagataacat
      121 cgagtgggca attaagcgct ttgccggtta ggctcaccaa agggttatcc atttctttag
      181 caacaagtgc gacattttct ttctcgtttt cttttttgaa gtggattttt ttgggatcac
      241 tggcattcga taagcatgtc agatcggaat gtaaaaacca ttctccggat ttattgatcc
      301 caattaacac aatttcatat ttttctttat ccattgcctc tacaatggtt tttgcagaaa
      361 gcaaagaaat ttcatgttcc gccgatcggc ctccaaaaag gacccctacg cgtatttttt
      421 tagacacagt taatcgcctt tctgagttta taaatttcaa tactaaatgc cattgatctt
      481 atcataatag atctgtctaa aatatcactc gttcaaacac tcagactgga tggggttatg
      541 gtggcggaac gggtggcgga gcgggtgacg gcgacgaaat gcttctagcg cctgcacccg
      601 tgcttcccgc ttctgaaaca gctaaaatat cgatagacgt cccttcaaca ggctcttgtg
      661 tgaaatgatt accaggagtg catcctgttg tcagtaaagc gagtccaatg aataacccca
      721 caaaaacttt ggttttattg accataacca ttgtccagtt ctccttaaat aaaagcttta
      781 tacctaaaca ttatctacgt ttaaaaattc caacacatca aaagagaagc attattttct
      841 gtatatcctt gctgtttaac taaaaaatca tagacacccg tgatagagag atcaggtctc
      901 acaaaatact taagagatgc gcctaaattt aaattgttct ttttattatt ttgagcacta
      961 gatgcaaaag gcgcgccgga agcggaaaat gtgaaacttg gattaagcag tggatttttt
     1021 aaatctctca gatagaatgc gcgaatttct gtcgtgacag ctggttgaat atacataact
     1081 cttccaccaa tccctgcttt tacaccatct gcagcgctgg agctgacaga aaggttagag
     1141 atatccgcgc ccgtttcgtt gcgaccactt tcgtgaaaat gtacataata caaagatgct
     1201 tcagggatga gctgccaatc agattgcctg ctcatatggc ctattcgaac cttactaaaa
     1261 aatgcagcac ctttaaaacg agaaagcgct tggagtgtag taccggaaat agcacgttta
     1321 gacttgctcc aactttcagt tcccgataaa aacaggccta aaaaataccc attttccact
     1381 aaatttgcat aacctaaaat ttgcccgtaa tcactattaa gcgtatctga tgtcgcaatg
     1441 tttgtatgac tctccgcgta ggttgcgcca ccccctattt caaacaaatc gagtgcggtc
     1501 ttaaaattat aatccaaaag atatccaaca ccaccgcttt taatatgata gccattgatg
     1561 tttgcagaaa catctgtctt ttgtcgttga aatgttccaa aaatgatagg ttcactttcg
     1621 taatccttta agtcatgtgt actggtaggc ctcgtttttt tttgaataaa tttataaa
//
LOCUS       DAORVT010000263         1657 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_85616_length_1657_cov_3.190387, whole genome shotgun sequence.
ACCESSION   DAORVT010000263 DAORVT010000000
VERSION     DAORVT010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85616_length_1657_cov_3.190387"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..140
                     /gene="eno"
                     /locus_tag="PK389_05425"
                     /pseudo
     CDS             1..140
                     /gene="eno"
                     /locus_tag="PK389_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414076.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; incomplete; partial
                     in the middle of a contig; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
     gene            223..504
                     /locus_tag="PK389_05430"
     CDS             223..504
                     /locus_tag="PK389_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007022078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HQY23172.1"
                     /translation="MKKGLALVLCLMFIVLQYRLWGGESSLLDVYRLRKAIVSEQKGI
                     VELTRRNQTLDAELKFLKLRPEALEERARAELGMIKKNETFCLVVEPAR"
     gene            515..1072
                     /locus_tag="PK389_05435"
     CDS             515..1072
                     /locus_tag="PK389_05435"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HQY23173.1"
                     /translation="MHFRFLVSWYDKILRWSAHPKATRYLAFVSFIDASLFPISPLFM
                     ILPMGIAEPKRAFQLAGIVIVSSFFGGILGYGLGLFAFDAIVNPFINMMGYSEYYQLA
                     AQWFQEWGFWAILFGCVTPFIPYKIFTIGAGVMQLHFGWFLLASLAGRSIRFLLIAAL
                     ILWGGPKVEPLFRKTLLKITNYQPS"
     gene            1192..>1657
                     /locus_tag="PK389_05440"
     CDS             1192..>1657
                     /locus_tag="PK389_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005297774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQY23174.1"
                     /translation="MGDNNTRLVSTIRDVTQIYLYEIASKSLLFAEEEKSLARKLQAG
                     DTIARKKMIEANLRLVVKIAHRYRHRGVSFSDLIEEGNLGLIHAVEKFDPERGFRFST
                     YATWWIRQAIDRAVMNQARMVRLPIHIIKDINSCFKVVKEFASEKSYFPTQEE"
BASE COUNT          471 a          283 c          387 g          516 t
ORIGIN      
        1 ttgcttcgaa tgcgagtcaa attaaagcgg gttctttgtc gcgtacagat agggttgcta
       61 aatataatca gctgctgcgt attgaagaag cattgggcaa tagcgcgcaa tatgcagggt
      121 atgcgccttt tgagcgtctt ctgacccgca cgaccctcac ccgcccttcg ggcaccctct
      181 cccgtaaaac gggagaggag agacagactg gagactagtt gggtgaaaaa agggctggca
      241 ctggttttat gtcttatgtt tattgttctc caatatcgac tgtggggagg agaaagtagt
      301 ttgttggatg tctatagact acgaaaggca attgtgagcg aacaaaaggg aatagtagaa
      361 ttaactagaa gaaatcaaac attagatgca gaactcaaat ttttgaagct acgcccagag
      421 gctttggaag agcgtgcacg tgcagaattg ggtatgatta aaaagaatga gactttttgt
      481 ttggtggtgg agccggcaag atagagagta taaaatgcat tttcgatttt tagtgtcttg
      541 gtacgataaa atattgcgct ggtccgcaca ccccaaagcg acgcgatatt tagcgtttgt
      601 tagttttata gacgcatcgt tgtttccgat ttcgccctta tttatgattt tgcctatggg
      661 tattgctgaa ccaaaacgtg cttttcaact tgcgggaatt gtgattgtgt cgtcgttttt
      721 tgggggcatc ctgggttatg gtctcggtct ttttgccttt gatgctatcg ttaatccttt
      781 tattaatatg atgggctata gcgaatatta tcaattggcc gctcaatggt ttcaggaatg
      841 gggtttttgg gctattttgt ttgggtgtgt aacacctttt ataccctata aaattttcac
      901 cattggcgca ggtgtgatgc aattacactt cggatggttt cttttagcat cacttgcagg
      961 tcgaagcatt cggtttttat taattgccgc gctaatactt tggggtggac ctaaggtcga
     1021 accattattt agaaaaacat tgcttaaaat tacgaattac caaccgagct aacagcgttt
     1081 tagtataaaa atgctttctt gtaaatacac cctgtaattt tcttaaaatc cgagtgtcta
     1141 atagccaagt aggatgattt tctgagacat atgagcaata cgaggtgttt aatgggtgat
     1201 aataatacac gattagtaag tacaatacgc gatgttacac aaatttatct gtatgaaatt
     1261 gcatcgaaat ccttgttatt tgccgaggaa gaaaaatctt tagcacgaaa attacaagcg
     1321 ggcgacacga tagcacgaaa aaagatgata gaagctaatt tacgtttagt tgtaaaaatt
     1381 gcgcatcgct atcgacatcg tggggtctca ttttccgatt taattgaaga aggaaattta
     1441 ggcttaattc atgcggttga aaaatttgat ccggaacgtg ggtttcgttt ttcaacttac
     1501 gcaacttggt ggatccgcca ggctattgat cgcgctgtca tgaatcaagc acgtatggta
     1561 cggttaccga tacatatcat aaaagatatt aacagttgct ttaaggttgt aaaagaattt
     1621 gcctctgaaa agagttattt tccaactcaa gaagaaa
//
LOCUS       DAORVT010000264         1641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_87167_length_1641_cov_2.778689, whole genome shotgun sequence.
ACCESSION   DAORVT010000264 DAORVT010000000
VERSION     DAORVT010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87167_length_1641_cov_2.778689"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /gene="tnpB"
                     /locus_tag="PK389_05445"
     CDS             <1..346
                     /gene="tnpB"
                     /locus_tag="PK389_05445"
                     /inference="COORDINATES: protein motif:HMM:NF017528.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HQY23175.1"
                     /translation="FTFSESIWLYPVPIDFRKQIDGIVIMVADHLNLNPASGQLFLFR
                     NKTQNKLKILWWDKNGFWLFYKRLEKGKFKFPAISAETIELSREQMGWLLSGLDYTKQ
                     PVPPKIEPTNFY"
     gene            400..>1641
                     /locus_tag="PK389_05450"
     CDS             400..>1641
                     /locus_tag="PK389_05450"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HQY23176.1"
                     /translation="MFDERDIRIAALEKENIELKKELNELRARYEQLSQNYEKLQHQL
                     NELLRHRFGKKSERFTEESSESNDDNEEGPSEDEPKEEDEWVLYRRKKKNKNKRDIPR
                     RALIIPVPEDQKQCACGCQKTFIRYETKELYNYQPAVFEIIEQKREVVACPKGCDNAI
                     VTAPAPLQVLPKIKVTEEFLSFLIVSKLDDRQPLYHLEQQLQERYGVECNRQKMARWL
                     IQLVEPIRPMFNLMKDETIAYDVSSCDATTLQVLREPGRAPETKSYVYCMRGGGPGKE
                     VVLYAYNNKLHKVFVQDWYTGYQGYLHVDGDNFFELIGSLAGISLVNCNSHARRKFEP
                     IAKGTKGQGLAKEAMRFFKSLYKIERTAKERALDYQARYELRQKEAKPILDDFKRWLE
                     EVYPTVLPKSPLGNAIRYCLNL"
BASE COUNT          546 a          285 c          348 g          462 t
ORIGIN      
        1 ttttactttt tcagaaagta tttggcttta tccggtgccg atcgatttta ggaaacaaat
       61 agatgggatt gttataatgg ttgctgatca tttaaattta aatcccgcgt ccggacaatt
      121 atttttattt cgcaataaaa cacagaataa gcttaaaata ttatggtggg ataaaaatgg
      181 tttttggtta ttctataaga gattggaaaa agggaaattt aaatttccgg caatttcagc
      241 agaaacgatc gaactatcca gggagcaaat gggttggtta ttatccggct tagattacac
      301 gaagcagcct gtcccgccaa agatagaacc aacaaatttt tattgaatgg ttaggcaatt
      361 tcttgataaa atgcctttca ggttatggaa atgaaaagta tgtttgacga acgcgatata
      421 cgcattgcag cacttgaaaa ggaaaatatt gagcttaaga aagagcttaa cgagcttcga
      481 gcgcgttatg aacaactttc tcaaaactac gaaaaattac aacaccaatt aaatgaatta
      541 ttacgtcatc gctttggtaa aaagtcagaa cggtttacag aagaatcctc agaaagcaat
      601 gatgataatg aggaaggccc ttctgaagat gaacctaaag aagaagatga atgggttctt
      661 tatcggcgca agaaaaagaa caaaaataaa agagatatcc caaggcgagc tctgattatt
      721 ccagtgccag aagatcaaaa acagtgtgct tgtggctgcc aaaaaacgtt tattcgttat
      781 gaaacgaaag agctatacaa ttatcaaccc gcagtatttg aaatcattga acaaaagcgg
      841 gaagtggttg cttgtccaaa aggatgtgat aatgctattg tgacagcccc tgcacctctg
      901 caagttctac ccaagatcaa ggtaacggaa gaatttttat cttttcttat tgttagcaag
      961 ttagacgatc gtcagcctct ttatcattta gagcaacaat tacaggaacg ttatggcgtt
     1021 gaatgcaatc gtcaaaagat ggctcgctgg ctgattcagc ttgtggaacc tataaggccc
     1081 atgtttaatt taatgaaaga tgaaacaatt gcttatgatg tttctagctg tgatgctacc
     1141 acgcttcaag ttttaagaga acccggcaga gctcccgaaa caaaatctta tgtttattgc
     1201 atgcgaggcg gagggccggg aaaagaggtg gtgctctatg cttacaacaa taaattacac
     1261 aaagtatttg ttcaagattg gtacactgga taccaaggtt atttgcatgt tgatggagac
     1321 aatttttttg agctgattgg ttctttggct ggaatatcat tggttaattg taactcccat
     1381 gcaagacgca aatttgaacc cattgccaaa ggaacgaaag gacaagggct tgctaaagaa
     1441 gcgatgcgat tttttaaatc cctttacaaa attgaacgta cagccaaaga gcgggcactt
     1501 gattatcagg ctcgttatga gctccggcaa aaagaggcca aacctatttt agacgatttt
     1561 aaacgctggt tagaggaagt ttacccaaca gtactgccaa aatcccctct gggcaacgct
     1621 attcgctatt gtttaaatct a
//
LOCUS       DAORVT010000265         1639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_87323_length_1639_cov_3.278409, whole genome shotgun sequence.
ACCESSION   DAORVT010000265 DAORVT010000000
VERSION     DAORVT010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87323_length_1639_cov_3.278409"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1639
                     /locus_tag="PK389_05455"
     CDS             <1..>1639
                     /locus_tag="PK389_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23177.1"
                     /translation="VTAPNLRARKALLSALETDEEIVFGNEYLILALVNPEPTTLQFE
                     LHMTDFLNVAPQNLTVNYQLDKTGTKSIQLTPNTPKSVLKVAVPKGEHALRINMENPF
                     VGQLLRVRMFEKDNKKIVQKLKIPYEVATQNDPIVAKIVGPAWVRIDEWRNGKIITNY
                     RYIASNTETLKLFPKQDQKEALYRLSARIPFSEKANILARPLNYITDPVPAAVCTSGY
                     YQPKEPLHYRLCNPLTIHNSTWSFRGLYNERIDIEEQGTFQSKIEQYFELNAAHRRYA
                     ENIKTYFNTKILSRKRKTGGNTFGLSEQIGYYPYLSSWSFHLDTYGFIQKPERNRTEW
                     STLFQGSITQKRDITPKAYHLPTLTTFKRLMSLSRTQNRLYSPGVLDQDIYTDYKSLH
                     RFGLEVSDTVVYRPKMDTLFLSNIGAVSRQDYRVYTPDHLKVSFDIKQLIGDYQLNAG
                     YRFQKFYKDKNKYVKFARKNNFKRRTVTLELSKKRWLGDHLFEFTLGTQRQVEFKKSM
                     FSLSFTYHFTHNRYYSDFSPRDSESEFVDIAYPQTQQ"
BASE COUNT          564 a          309 c          290 g          476 t
ORIGIN      
        1 aagtgactgc acctaattta agagcacgca aagcgttgtt atcggcactt gagactgatg
       61 aagaaattgt ttttggaaat gaatatctca ttttggcgct ggttaatccc gagccaacta
      121 ctttacagtt tgagttacac atgacagatt ttttaaatgt tgctccgcaa aatctcactg
      181 taaactacca attagacaaa acgggtacaa aaagcataca attgacacct aacacgccta
      241 aaagcgttct aaaagttgcc gtgccaaagg gggaacacgc acttcgaatt aacatggaaa
      301 acccattcgt ggggcagttg ttgagagtgc gcatgtttga aaaagacaat aaaaaaatag
      361 ttcaaaagct gaaaatacct tatgaagttg caacacaaaa tgatccaatt gtcgctaaaa
      421 tagtagggcc cgcttgggtt agaatagatg aatggcgcaa tggaaaaata ataaccaatt
      481 atcgatatat tgcttccaat acagaaacac ttaaattatt tccaaaacaa gatcagaaag
      541 aagcgcttta tcgtttgtct gcacggattc cttttagtga aaaagcgaat attctagcac
      601 gtcctttaaa ctatataact gatcccgttc cagcagcagt gtgcacgagt ggatactatc
      661 aaccgaaaga gcctttacac taccggttgt gcaatccatt aaccattcat aatagcacct
      721 ggtcattcag aggtctctat aatgaacgaa tcgacataga agaacaaggc acttttcaat
      781 ctaagattga acaatatttt gagttaaacg ccgcacatcg acgttatgcc gaaaatatta
      841 aaacatattt caataccaaa atattaagca gaaaacggaa aacaggtgga aacacgtttg
      901 gattatcaga acagataggt tattatcctt acctctcttc ttggtcattt catttagaca
      961 cctatggttt tattcaaaaa ccagagcgta accgaacaga gtggtcgact ttatttcaag
     1021 gttcaatcac acaaaagaga gatataactc ctaaggctta tcatctacct acactgacca
     1081 cttttaaacg attgatgagt ttaagtcgaa cgcagaatcg gttgtattcc ccaggtgttt
     1141 tagatcaaga tatttatacc gattataaat cattgcatcg atttggcctt gaagtatctg
     1201 atacggtggt gtatcgacct aaaatggata ctttatttct ttcaaatatt ggcgcagttt
     1261 cgcgtcaaga ttatcgtgtt tatacaccgg atcacctaaa agtcagcttt gacattaaac
     1321 aactcatagg cgattatcaa ttaaatgccg gttatcgatt tcaaaagttt tataaagata
     1381 aaaacaaata tgttaaattt gcacgtaaaa ataattttaa acgtcgaact gttaccttag
     1441 agcttagtaa aaagcgttgg ttaggagatc atctttttga atttacatta gggacgcaaa
     1501 gacaagttga gttcaagaaa tctatgtttt ccttatcatt tacctatcat ttcacccata
     1561 atcgatacta ttctgacttt tcgccaagag attcagaatc agaatttgtg gatattgctt
     1621 atccccaaac gcaacaatg
//
LOCUS       DAORVT010000266         1628 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_88362_length_1628_cov_5.089638, whole genome shotgun sequence.
ACCESSION   DAORVT010000266 DAORVT010000000
VERSION     DAORVT010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88362_length_1628_cov_5.089638"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="PK389_05460"
     CDS             complement(<1..979)
                     /locus_tag="PK389_05460"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HQY23178.1"
                     /translation="MEKRILQSVINMTEQRDVDSLEYGVVVTLAELLPLNSISLYKLV
                     GDKPEDGIEEVICLFAGENGEIQARYKQNNEVNIVKENQYLYSCLLDRKEVLYKNGNG
                     QTHMISPLICDKKIIGAIEIASNKDLKKFKELINGITRIYENYLYILNKSERDKLTGL
                     LNRHSFDTKFSRLLQIQKDEKQSDSKSQVTNERRQQATDSHAWLAIIDIDHFKNVNDK
                     FGHVCGDEVLLMLSQRMKAFFRSSDLLFRFGGEEFVIILEPIPHEMAERTLERFRNKV
                     ADSNFPLIEKITISGGYIKITDLHYPVTLIERADQALYYAKKNGRNCMYS"
     gene            complement(1455..1607)
                     /locus_tag="PK389_05465"
     CDS             complement(1455..1607)
                     /locus_tag="PK389_05465"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23179.1"
                     /translation="MSIGVAANMDGGMRFERPEDLIEAADLALYAAKQAGRNAIIEWN
                     QSLQLK"
BASE COUNT          465 a          342 c          263 g          558 t
ORIGIN      
        1 agctgtacat acaattacgc ccattcttct tggcgtaata taatgcttga tctgcccgtt
       61 cgattaatgt aacagggtaa tgcaaatcag tgattttgat atatccacca ctaatagtga
      121 ttttctcaat cagtggaaaa ttactatcag ctactttatt acgaaatcgt tctagtgtac
      181 gttcagccat ttcatgtggg ataggttcta aaataatcac aaattcttcg ccaccaaagc
      241 gaaaaagcag atcagagcta cgaaaaaaag ctttcattct ttgagatagc ataagaagta
      301 cttcgtcacc gcatacatgt ccaaacttat cattaacatt cttaaagtgg tcgatatcaa
      361 taatcgctaa ccatgcatgt gaatcagttg cttgctgtct tctttcattt gtaacttgac
      421 ttttactatc tgattgtttt tcatcttttt gtatttgtaa taaacgacta aattttgtat
      481 caaagctgtg tcgattaagc agacccgtta acttatctcg ttcgctctta ttcaagatat
      541 acaaataatt ttcataaatt cgtgtaatgc cattaattaa ttctttaaac ttctttaaat
      601 ctttgttact tgctatctct atagcgccaa ttatcttctt atcacaaatt aaagggctaa
      661 tcatatgcgt ttgaccattg ccatttttgt ataaaacttc ttttctatct aacaagcagc
      721 tatataaata ttgattctct ttaacaatat ttacctcatt attttgctta tatctggctt
      781 gaatctctcc attctctcct gcaaatagac aaataacttc ttcgatgcca tcttctggtt
      841 tatcacccac caacttatat aaggaaatag aattcaatgg taatagctcg gctaatgtaa
      901 caactactcc atattcaagc gaatccacat cgcgctgttc tgtcatattg atgacagatt
      961 gcaaaatcct tttttccatt ctcagaactc cccatcattt agttatttga taataatatc
     1021 aggtatatac tatattctag cgatgaattg ctatcttcta gggataatgg aatactatga
     1081 atatagtaaa aaaatgtgtt ttgtcgtgac cagttcctgg ttaatgatgg attgagtgtt
     1141 tctttttcgt ttcatcggca gcgtccagat cccatctcca ggatatctag ttgctggaat
     1201 gcttcgcaga tatttacgcc tgggatacca cttgatgctt ttgagcagtt tcaatgtggc
     1261 tctctcaatt ctcacttctc atcaccctcg ccaaccttca taggcaggcc cttggcggtc
     1321 tggtagagtg catcgtgaca tggaatcaat caggcagttg cttggcttcc gggttgagag
     1381 taagctgaag tttgcgcacg gtttctccgc catcagagaa atagtcagaa tccacactta
     1441 atagatttat caatttattt aagttgaagc gattgattcc attctataat cgcatttctc
     1501 cccgcttgct tggcagcata aagagcaaga tcagcagctt ctattagatc ttccgggcgt
     1561 tcaaaacgca ttccaccatc catgtttgca gcgaccccaa ttgacacagt cacggtaaca
     1621 aaacgttg
//
LOCUS       DAORVT010000267         1628 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_88381_length_1628_cov_3.428481, whole genome shotgun sequence.
ACCESSION   DAORVT010000267 DAORVT010000000
VERSION     DAORVT010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88381_length_1628_cov_3.428481"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..760)
                     /locus_tag="PK389_05470"
     CDS             complement(143..760)
                     /locus_tag="PK389_05470"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HQY23180.1"
                     /translation="MHYKIVIASTNEGKIREIHKILQELDGLKMDLRSLKDYNIPEPD
                     EPHQSFMGNAIHKAKYYAQYTKLPTLSEDSGLSIEALNGFPGVRTKEFGTECDGRERG
                     LAKTFIRLAEMLKETGNYTACFNSATVLYIPTYDYLITHEAKDDGTLTFPPRGDDGFA
                     YDPVFVPKGYDKTFAELGLEIKNKISHRAQAMQGLIEKFRQFLAN"
     gene            complement(767..>1628)
                     /locus_tag="PK389_05475"
     CDS             complement(767..>1628)
                     /locus_tag="PK389_05475"
                     /inference="COORDINATES: protein motif:HMM:NF015922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HQY23181.1"
                     /translation="LDSDQRLKTVLKKELEQDLKTYGDKRRSPVVVRFDAKVLEEIDK
                     PPAESVTIILSSKGWVRSAKGHEIDPTTLSYKTGDEFKLAVAARSDQPVVFLDSTGRA
                     YTLAVQSLPSARGQGEPLTGRINPPPGVTFDSVLVGENTDRYLLMSNAGYGFIIPFSE
                     LLAKNKNGKAIVKIKEGQRLLPALKVSDNKALVAVATGDGRLSLFPLSAVPDMTKGKG
                     NKLMGVKKESSIIYAAVLMPNQTLTVLAGRKKLELKPKEWKPYLAEAGSRGIKLPKGF
                     EKVQNLIVEA"
BASE COUNT          433 a          377 c          294 g          524 t
ORIGIN      
        1 gttaaactta cgctgctaaa gtggattttt aaccaaaatg cccccgtgtt ggattatgag
       61 atattttgta caactagctc aacatgaaat actggttatt tattcacctt atttaatcac
      121 aaagcgatcg gagcagaatg ctttaatttg ctaaaaattg ccgaaatttt tcgattaatc
      181 cctgcatggc ttgcgcccga tggctaatct tatttttgat ttccaatccc aattctgcaa
      241 aggttttgtc gtaccccttt ggcacaaaga cgggatcata agcaaaacca tcgtctccac
      301 gcggtggaaa agtcaaagta ccgtcatctt tcgcttcatg tgtgattaaa taatcataag
      361 ttgggatata taaaacagtc gcactattaa aacaagcagt ataattacct gtctctttta
      421 gcatttctgc taatcttata aatgtctttg ccaatccacg ctctctgcca tcacattcag
      481 tcccaaactc ttttgttcta acacccggaa atccatttaa agcttcaatg cttaagcctg
      541 aatcttctga taaagtcggt aactttgtat actgtgcata atattttgct ttatgaatgg
      601 catttcccat aaagctctga tgcggttcgt cgggctcagg gatgttgtag tcttttaacg
      661 atctcagatc catttttaag ccatctaatt cctgtagaat tttgtggatt tctctgattt
      721 ttccttcatt agttgaggcg atcacaattt tataatgcat gtattattac gcctcaacaa
      781 taaggttttg aactttttca aaacctttag gtaacttaat cccacggctc cctgcttcgg
      841 ctagataagg tttccactct ttgggtttta attccagctt tttgcgaccc gccaataccg
      901 ttagtgtttg atttggcatt aatacagcag cataaataat gctgctttct tttttaacgc
      961 ccattaattt attgccttta ccttttgtca tatcgggcac tgcagacaat gggaataacg
     1021 agagacgtcc gtctccagtc gcaacagcaa ccaatgcctt attgtcggaa accttcagcg
     1081 caggcaataa ccgttggcct tcttttatct ttacaatcgc tttaccattt ttatttttag
     1141 ccagaagctc tgaaaacgga ataataaaac cataacccgc attagacatt aacaaataac
     1201 ggtcggtatt ctcgcccacc aacacgctat cgaaagtaac acccggtggc ggattaatac
     1261 gccccgtcaa tggctcgccc tgcccacggg cagacggcaa actttgtaca gctaaggtat
     1321 aggctcttcc ggtggaatcc aaaaatacaa cgggttgatc gcttctggct gcaaccgcaa
     1381 gtttaaattc atccccggtt ttgtaactca gtgttgtggg atctatttcg tgcccttttg
     1441 ccgaacgcac ccatcccttg ctagacaata taatcgtcac tgactctgca ggcggcttat
     1501 caatctcttc tagtactttg gcatcgaagc gtacgacaac tggagaacgt cgcttatcgc
     1561 cataggtttt gaggtcttgc tctaattctt ttttgagcac cgtttttaag cgctgatctg
     1621 aatccaag
//
LOCUS       DAORVT010000268         1628 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_88449_length_1628_cov_1.682772, whole genome shotgun sequence.
ACCESSION   DAORVT010000268 DAORVT010000000
VERSION     DAORVT010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88449_length_1628_cov_1.682772"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PK389_05480"
     CDS             <1..358
                     /locus_tag="PK389_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23182.1"
                     /translation="ARAQARRRAVGNRQLPQQTTMPAIVFTRTNTMRDDIAHSGAMGL
                     AQAIIQVSIFSETSLDARNLSDAIRKLFHVYKGTVNTVQILLSKVMNEIDLYDSETEI
                     YHTVLDLDIKYREPTS"
     gene            432..848
                     /locus_tag="PK389_05485"
     CDS             432..848
                     /locus_tag="PK389_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23183.1"
                     /translation="MGTPAAAHGALIKIGNGASSETFATIDDVTAGPDGGGLSTTMLT
                     ARVHSQASEVKRVSGTTTEPISFTVLYDSADTQHALIKTNAAAKTRTNFQIVGADFGA
                     EQMAFAAYIGYNFSKNPDGWDEMSVTLEIDGDVTFT"
     gene            865..1242
                     /locus_tag="PK389_05490"
     CDS             865..1242
                     /locus_tag="PK389_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23184.1"
                     /translation="MSDSLEKLKPSITVSLLGRERSFRFSLKSCAQFQKLTKKSLLSG
                     GIDIYDAEGLATLLWAGLIPDNKEFDGRIDSDGKPDETIQKTIDELLESLDLSDYAYL
                     VNEVIGKAIQLAQPKEDPKKKGK"
     gene            1266..>1628
                     /locus_tag="PK389_05495"
     CDS             1266..>1628
                     /locus_tag="PK389_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23185.1"
                     /translation="MGRSNPAKKTNDRFSTNWFVFDIGFANYNDKTDYANTGNYLVNS
                     PGGLAWSKSDFKLRTGKSINVNIWFFMQTMHLVKKNVNLKYGLGLELNNYRFKSNISY
                     KEDGPIPYSGGMQTNDAFI"
BASE COUNT          537 a          311 c          345 g          435 t
ORIGIN      
        1 ggcgcgtgct caagcccgac ggcgcgctgt gggtaatcgg cagctaccac aacagacaac
       61 catgcccgcg attgtattta ctcgaactaa cacaatgcga gacgacattg cacacagtgg
      121 ggcgatggga ctagctcaag ccatcataca agtgtcaatc ttttcagaaa caagcttaga
      181 tgctcgcaat ttatcagacg caatcagaaa gctttttcac gtctataaag gcacagtcaa
      241 caccgttcaa atactactat caaaggtcat gaacgagatt gacctttatg attccgaaac
      301 agagatttat cacaccgttt tagatctcga tattaagtat agggaaccaa cttcctaggc
      361 tttagggcta ttttttcgga cttatcccat taaaatttct ttgaaacttt agtttttgga
      421 gattttaaac aatgggaaca ccagcagcag cacatggcgc gttaatcaag ataggtaacg
      481 gcgcttcaag tgaaactttt gcaactatag atgatgtaac agcgggtcca gatggcggcg
      541 gtcttagtac tactatgctt acggcgcgag tacacagtca agcatctgaa gtaaaaagag
      601 ttagtggaac aactacagag ccaatttctt ttacagttct ttacgattcc gcagacactc
      661 aacacgcact tattaaaaca aatgcagccg caaaaactcg cactaacttt caaatagttg
      721 gcgctgattt tggtgcggag caaatggcat ttgccgcata cattggttac aacttttcaa
      781 agaaccccga cggatgggat gaaatgtcag tgactttgga aatcgacggt gatgttactt
      841 ttacttaata gaatagggga ttaaatgtca gactcgctag aaaaacttaa accatcaata
      901 actgttagtt tattagggcg tgaaagaagt ttcagatttt ctttaaaatc atgcgctcaa
      961 tttcaaaagc taactaagaa aagtttatta tccggcggga ttgatattta tgacgcggaa
     1021 ggattagcca cgcttttatg ggcggggcta atccctgata ataaggaatt tgacgggcga
     1081 attgactcag atggcaagcc agacgagaca attcaaaaaa ccattgatga attattagag
     1141 agccttgatt tatctgatta tgcttaccta gttaatgaag taatcggaaa agcgattcag
     1201 cttgcacagc ctaaagaaga tccaaaaaaa aagggaaagt aaaaaatgca gatagcagtt
     1261 ttgtaatggg taggagcaac cctgctaaaa aaacaaatga tcgttttagt acaaactggt
     1321 ttgtatttga tattggcttt gcgaattaca atgataaaac cgattatgcc aacacaggca
     1381 attacctggt aaacagcccc ggtggattgg cctggagtaa aagcgatttt aaattacgca
     1441 ctggtaaaag catcaatgta aatatttggt tttttatgca aaccatgcac ctcgtaaaaa
     1501 agaatgtgaa tttaaaatat gggctgggcc tggagctgaa taattatcgt tttaaatcaa
     1561 atatcagcta taaagaagat ggcccaattc cttatagcgg gggcatgcaa accaatgacg
     1621 cattcatt
//
LOCUS       DAORVT010000269         1620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_89179_length_1620_cov_3.371246, whole genome shotgun sequence.
ACCESSION   DAORVT010000269 DAORVT010000000
VERSION     DAORVT010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89179_length_1620_cov_3.371246"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PK389_05500"
     CDS             complement(<1..424)
                     /locus_tag="PK389_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23186.1"
                     /translation="MAGFAFVDLLPAILISGGIVGGLGVLCCVATAFVHPKSLTNSIL
                     QASILQKPHFKIKQVCRLWLGQIVFIGMSFVLFHFLETQTQLNTRLSFVETGYIMGVW
                     QQNPFGLGLLPFIIYAVLGLGLAYFSICLGEKPILTRVF"
     gene            complement(507..1556)
                     /locus_tag="PK389_05505"
     CDS             complement(507..1556)
                     /locus_tag="PK389_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23187.1"
                     /translation="METNKKRTGRFGAALLTVCIMGILFGWTAYAEDSPAVDKDVGDV
                     AQKVLTGSPLTEPPPAPPLGISAPPVPPGILDRATHFMEQLSEGVLLLINNNTNSALY
                     GAGIEASKAMVAKHFDRYCAGLETGNCPSDPLLQNGDIKISSILSGTAYDGPARVQAA
                     QDYLTNLFVPPSAPLVANFSGDLKDGKLDVATVVGDPKLLKKYTQALSDETVLSAARQ
                     SFAQMMAMRTVSTADAGSISEMQLMEAEGIKRFMSTGWVQMIKDPKTTPAQLQQEMVA
                     MQAYQNWMAYQQFRQLERIEALLATVIVQSARTMQTIGTSLPTAPSAAEINNAATSTG
                     AVPPSPQSAIESSSP"
BASE COUNT          470 a          404 c          312 g          434 t
ORIGIN      
        1 aaaaaacccg cgttaaaatc ggtttttcgc ctaggcaaat gctaaaataa gctaaaccca
       61 accctaaaac agcataaata ataaagggca acaaccctaa tccaaaagga ttctgctgcc
      121 atactcccat gatatatccg gtttctacaa aagataagcg cgtgttaagc tgggtttggg
      181 tttctaaaaa atgaaataaa acaaaagaca tgccaataaa aacaatttgc cctaaccata
      241 atcgacacac ttgctttatt ttaaaatgtg gcttttgaag tatcgaagcc tgcaatattg
      301 aatttgtaag agatttcgga tgtacaaaag ctgttgccac acaacacaac acaccaagtc
      361 caccgacaat cccaccactt atcaatatcg caggcaataa gtcgacaaat gcaaaacccg
      421 ccacgcagaa gcctatcgct aacaacaaat aagaaatata gctagtgaat gttacacggc
      481 gttttaattt gataacactc ctcctactac ggactcgagc tttcgatcgc gctttgggga
      541 gacggcggta cagcacccgt actcgtagct gcattattga tctcagcagc tgaaggcgct
      601 gttggaagac tcgttccaat cgtctgcatt gtacgcgcac tttgaacaat gacagtggct
      661 aataacgcct caatgcgctc caattgtcta aattgttgat aagccatcca gttttgatat
      721 gcttgcatag ccaccatttc ttgctgaagt tgcgccgggg tcgtctttgg atctttgatc
      781 atttgcaccc aacctgtgct cataaatcgc ttaattcctt ctgcttccat taactgcatt
      841 tcagaaatgc tacccgcatc tgcggttgat accgttcgca ttgccatcat ctgcgcaaac
      901 gattgcctgg cggcagagag cactgtctca tcagataatg cttgagtata ttttttcaat
      961 aatttgggat ccccaacaac cgtcgcgaca tccaacttac cgtcttttaa atcgccgcta
     1021 aaattggcca ctaatggcgc gctgggaggc acaaataagt tcgtgagata atcttgggca
     1081 gcttgtacac gcgcaggacc atcataagct gttccggaaa ggatgctact gattttaatg
     1141 tccccatttt gtagcaaagg atcgctagga caattccccg tttccagacc cgcacaataa
     1201 cgatcaaagt gtttcgcaac cattgcttta gaggcttcta tccctgctcc gtacaacgca
     1261 ctgtttgtgt tgttattgat caacaacaga acaccctcgc tcaattgctc cataaaatgt
     1321 gttgccctat ccaaaatgcc aggaggcaca ggtggcgctg aaatacccaa aggtggagct
     1381 ggaggcggtt ctgttaaggg ggaaccggta agtacttttt gagccacatc tcccacatct
     1441 ttatcgacag caggcgaatc ctctgcatag gctgtccagc caaataaaat acccatgatg
     1501 caaacagtca ataacgctgc gccgaaacgc cctgttctct ttttatttgt ttccattact
     1561 ttacctcctt gtacgctatt cactctgttg ttccaatgct tttaaaatca aagaaactcg
//
LOCUS       DAORVT010000270         1616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_89597_length_1616_cov_3.659833, whole genome shotgun sequence.
ACCESSION   DAORVT010000270 DAORVT010000000
VERSION     DAORVT010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89597_length_1616_cov_3.659833"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /gene="minC"
                     /locus_tag="PK389_05510"
     CDS             <1..569
                     /gene="minC"
                     /locus_tag="PK389_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011395662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HQY23188.1"
                     /translation="QQLVGILRSKKLIPVGIKGGTTEQQAAATLVGLPILQDSGKNPV
                     KTETRNTSVASSAGKLPTPTEALPTTDQETDTEIRGSNTKLITEPVRSGQQIYARGGD
                     LIVLAPVSHGAELLADGHIHIYGPLRGRALAGVTGDETARIFCQYLEAELISIAGQYK
                     ISEDIEQTVWRMATDISLKEGHLHIQPL"
     gene            617..1459
                     /gene="minD"
                     /locus_tag="PK389_05515"
     CDS             617..1459
                     /gene="minD"
                     /locus_tag="PK389_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006646961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HQY23189.1"
                     /translation="MLTNETRLQKSGKVIVVTSGKGGVGKTTTSAAIAAELANRGFKT
                     VVIDFDIGLRNLDLIMGCERRVIFDFVNVIQGDANLNQALIKDKRSDNLYTLPASQTR
                     DKDALTKEGVEKILSLLRLEFDFIICDSPAGIERGALMALYFADEAVVVTNPEVSSVR
                     DSDRIIGVLNSRTLCAEKGLEVKKHLLLTRYAQSRVQKGEMLSVEDVQEILAIPLLGI
                     IPESKAVLNASNNGVPVTLGSPSDAKVAYQKAVSRLLGEVNQPAKSWKSKFLGGLRLF
                     RSPA"
     gene            1456..>1616
                     /locus_tag="PK389_05520"
     CDS             1456..>1616
                     /locus_tag="PK389_05520"
                     /inference="COORDINATES: protein motif:HMM:NF015717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HQY23190.1"
                     /translation="MSIFRKLLNKIWLARHSAKTARERLQIVVSHQRAEEGGGVDFIS
                     RLRNELLGG"
BASE COUNT          488 a          345 c          360 g          423 t
ORIGIN      
        1 atcaacagct tgtcggcatt ttgcgcagta aaaaattaat ccctgtcggt attaaaggtg
       61 gcaccacaga acagcaagcg gcagcaacac ttgttgggtt gcccatcttg caagactccg
      121 gcaaaaatcc agtcaagaca gaaacaagaa acacgtctgt agcaagttct gctggaaaat
      181 tgccaacacc cactgaagcg ctgcccacca cagatcaaga aacagacact gaaatacgcg
      241 gcagcaacac caaactgatt accgaacctg tgcgctccgg acaacaaatt tacgcacgag
      301 gtggtgattt aatcgtactc gcacccgtaa gccatggtgc agaattgctg gcagatggcc
      361 atattcacat ttatggtccc cttagaggcc gcgcattagc cggcgtgaca ggcgatgaga
      421 ctgcacgtat attttgccaa tatctagaag ctgagcttat ttctattgca gggcaatata
      481 aaatcagcga agatatcgaa cagaccgttt ggcgtatggc aaccgacatc tctttaaaag
      541 aaggtcactt gcacattcag ccactttaat atatatagtc tttgtagaaa ctaaaaaata
      601 cctttggaag gattgtatgc tcacaaatga aacccggtta caaaaatctg gaaaagtaat
      661 cgtagttact tccggtaaag ggggcgttgg taaaacaacc accagtgctg ctattgctgc
      721 agaattagcc aatagaggct ttaaaactgt tgttattgac tttgacatcg ggcttcgaaa
      781 tttagatctc attatgggat gcgaaagacg ggttattttt gattttgtga atgttattca
      841 aggagatgct aaccttaatc aagcgctgat taaagataaa cgttccgaca acttatacac
      901 tctacccgcc tcacaaacac gtgataaaga cgcgcttacc aaagaaggtg ttgaaaaaat
      961 attatcgctt ttacggctag aatttgattt tattatttgt gattctcctg caggcataga
     1021 gcgcggcgct ttaatggcgc tgtactttgc agacgaggcc gtcgtcgtca ccaacccaga
     1081 ggtttcttct gtacgcgatt ctgatcgcat tatcggcgtt ctaaacagtc gaaccctatg
     1141 cgccgaaaaa ggtttggaag ttaagaaaca tttgttatta acacgatacg cccaaagtcg
     1201 tgttcaaaaa ggcgagatgc tgagtgtaga agatgttcaa gaaatcttag ctatcccctt
     1261 actcggtatt attccagaat ctaaagcggt tctaaatgca tcaaataatg gtgtgcctgt
     1321 tacattagga tctcccagtg atgctaaggt tgcttaccaa aaagccgtct cacgcctttt
     1381 gggtgaagtg aaccaacctg cgaaaagctg gaaatcaaaa ttcttagggg gcttacgcct
     1441 atttaggagc ccagcatgag catcttcaga aaattgttaa ataaaatttg gcttgctcgg
     1501 cattcggcaa aaacagcgcg agaacgatta caaattgttg tgtctcatca acgtgcagaa
     1561 gaaggtggcg gtgtggactt tatttcccgg ttgcgtaatg aattactcgg tggtat
//
LOCUS       DAORVT010000271         1615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_89714_length_1615_cov_2.937179, whole genome shotgun sequence.
ACCESSION   DAORVT010000271 DAORVT010000000
VERSION     DAORVT010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89714_length_1615_cov_2.937179"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PK389_05525"
     CDS             complement(<1..444)
                     /locus_tag="PK389_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQY23191.1"
                     /translation="MPIKPDKRNYLIESAKKLIYKQGFNATTLADIAHEANVPLGNVY
                     YYFKTKADIGLAVLNSISAEQKIFLGHLVKEPSASVRLHYFLEHTNQERKLIMLQGCR
                     IGTLCQEFAKEGGMLHKLAASIMMDALLWIEAQFYTLGFVEEASDL"
     gene            complement(880..>1615)
                     /locus_tag="PK389_05530"
     CDS             complement(880..>1615)
                     /locus_tag="PK389_05530"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HQY23192.1"
                     /translation="HNRAIEEGTGGAKDYLRVQPVAKTYEVCVPRRKGEYPERTATVE
                     LRYAPITVYAPDDEEVVHDEIKMYGIHVKEINPPSDVEPIEWFLVTNVAVLKTEDALE
                     KVRWYLLRWMVEIYHKSEKSCCSSEDCRLETDERMLNFLAVNSVIAWRVLFVTYIARQ
                     QPEAPAEVILAPTEIKILEAANNAKLPKPTKIKTVKQAVQALAKLGGHMGRKNDKAPG
                     IITVCRGMMRLNDFIYGFDLSVQLKA"
BASE COUNT          467 a          361 c          265 g          522 t
ORIGIN      
        1 taaatcagac gcttcttcta caaatccgag tgtataaaat tgcgcttcta tccaaagcaa
       61 agcatccatc attatacttg cagccaactt gtgtaacata ccgccttcct ttgcgaattc
      121 ttggcaaaga gtacctatac ggcagccttg aagcatgatt aatttgcgtt cttgattcgt
      181 atgctctagg aaataatgca aacgcacgct tgcagaaggc tctttaacca aatgtcctaa
      241 gaatattttt tgctcggctg atatcgaatt aagaacggct agaccaatat ctgctttcgt
      301 tttaaagtag taatacacat tgccaagtgg tacatttgct tcatgagcaa tatccgcgag
      361 cgtggttgca ttaaaacctt gcttataaat tagctttttt gctgactcta ttaggtaatt
      421 acgcttatca ggctttatag gcatgttact ctcccttaat tcatacgagt cttatatttg
      481 gtctcttctt tcgggcccgg ataccaaaag cccatagaca ataaaaagaa atacattttt
      541 cgtaatggga ttttattaaa aaataatact tttgaaggca ctgacacata aataccagcc
      601 tgaatctctg gtgtaaacga ctttttgatt gagtgttatt atttttagaa acttgatgcg
      661 taacattttg tgcggtgtcc atattcccct tccctatcga ttaacgcacg ggaatcgtat
      721 gttctttcac acaaaaaagc aacaaatttt ttataaaacc ctagctgact aatagttcat
      781 acaaccaggc gagacatgta ttgcctaaac cagcatattc tagccctttt gacctcttgt
      841 aggttttcct attaccaccc tatctattac ccataaatat taagctttca actggactga
      901 caagtcaaag ccataaataa aatcgttgag cctcatcatg cctctgcata ctgtaataat
      961 gcctggtgct ttgtcattct ttcttcccat gtgtccacct agttttgcca aagcttgaac
     1021 agcttgttta acagttttaa tttttgtagg ctttggcagt tttgcattat tggcagcctc
     1081 taatatttta atctcagttg gagccaaaat aacttcagca ggagcttctg gttgttgccg
     1141 ggcaatatat gtaacaaata atactcgcca agcaatcaca ctattgactg ctaaaaaatt
     1201 tagcatacgt tcatctgttt caagtcggca gtcttcgcta ctgcaacaac ttttttcgga
     1261 tttatgataa atctccacca tccaacgcaa taaataccaa cgaacctttt caagagcgtc
     1321 ttctgttttt aaaacagcaa cattggttac taaaaaccat tcaatgggtt caacgtctga
     1381 aggtggatta atctccttaa catgaatgcc atacatttta atttcatcgt gaaccacttc
     1441 ttcatcatcc ggcgcataaa cagtaatggg cgcataacgc aattcaacag tcgctgtacg
     1501 ttctggatac tcgccttttc gtctcggaac acagacctca taagttttcg caacgggttg
     1561 aaccctcaaa taatctttcg cacccccagt tccttcttca atcgcacgat tgtgt
//
LOCUS       DAORVT010000272         1606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_90577_length_1606_cov_6.521599, whole genome shotgun sequence.
ACCESSION   DAORVT010000272 DAORVT010000000
VERSION     DAORVT010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90577_length_1606_cov_6.521599"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..451)
                     /locus_tag="PK389_05535"
     CDS             complement(2..451)
                     /locus_tag="PK389_05535"
                     /inference="COORDINATES: protein motif:HMM:NF023087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23193.1"
                     /translation="MSAAERKLDEAIQHIASVHGIVVSKDDPVMILHTLNERLIQDSK
                     AAQQDLLDNFKGEIELIVSELSMAAKNHSDRVLNSTIETSKTEIARVMEEQSHIIIER
                     WKADLNAGFHEASKTMATSRQTAILNIIASFITSISAVIILYVFLTM"
     gene            complement(448..1179)
                     /locus_tag="PK389_05540"
     CDS             complement(448..1179)
                     /locus_tag="PK389_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HQY23194.1"
                     /translation="MAKVHMILQGKGGVGKSLIASILAQHCYAKKKDILCIDTDPVNA
                     TFHGFKKLNVTRLDLMDGDNVDPRKFDHLVEMITKSKSDVIIDNGASTFVGLSHYLIS
                     NQVSTMLREMGHELVIHTVITGGQSLTDTVNGFNRIIQQFPTDVTFIVWLNQFWGKIE
                     MNGKKFESMKAYVDNKSRISAIVTIPEYKMETFGRDLREMLQDKLTFDEAIAKPEILI
                     MVRQRLKIIKEDLFKQLEGAQAVLA"
     gene            complement(1305..>1606)
                     /locus_tag="PK389_05545"
     CDS             complement(1305..>1606)
                     /locus_tag="PK389_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23195.1"
                     /translation="LHDEGKMVISYQVFLRLVKKHTDYKSHKRSTKKSELESEVEEKQ
                     NNRSDDTHKESLSENKKDLEPAQEKTDSPKSPKYPDTGNPKGFEWSADYNVDDII"
BASE COUNT          439 a          343 c          285 g          539 t
ORIGIN      
        1 tctacatagt taaaaacacg taaagaataa tgacagcaga aattgacgtg atgaatgagg
       61 caatgatatt taatattgcg gtttgtcgtg acgttgccat tgttttggaa gcttcatgaa
      121 acccggcatt caaatcggct ttccagcgct caatgatgat atgggattgt tcttccatca
      181 cgcgcgcaat ctctgttttg ctagtttcta tggttgaatt aagaactcga tccgaatgat
      241 ttttagctgc catggataat tcagaaacga tcagctcaat ttctcccttg aaattatcca
      301 gtaaatcctg ctgtgcagcc ttgctatcct gaatcaggcg ctcattcaag gtatgtaata
      361 tcatgaccgg atcatcttta gatacgacga tcccatgaac ggatgcaatg tgctggatag
      421 cctcatccag ctttcgttct gcagcactca tgccaatacg gcttgtgcac cttccaattg
      481 cttaaataga tcttctttaa ttattttaag ccgctggcga accataatta aaatttctgg
      541 ttttgctatc gcctcatcaa aagtcaattt atcctgtaac atttccctca aatcacgacc
      601 gaaagtctcc atcttatatt ccgggatagt tactatggct gatatgcggc ttttattatc
      661 aacataggct ttcatagact caaatttctt gccattcatt tcaatctttc cccagaactg
      721 gttaagccag acaataaatg taacatcagt aggaaattgt tgaataattc tattgaatcc
      781 attgacagta tccgtcagag attgaccgcc tgtaatcacg gtatggatta ccaactcatg
      841 ccccatttcc cgaagcattg tagaaacctg attgctgatc aaataatgcg ataaacccac
      901 aaatgtgctg gcgccattat cgatgatcac atcagatttg cttttggtga tcatctcaac
      961 cagatgatca aatttacgtg gatcgacatt atcgccatcc attaaatcca accgcgtaac
     1021 attcagtttc ttaaacccat gaaaagtcgc attcaccgga tcggtatcga tgcagagaat
     1081 atcttttttc ttggcgtaac aatgctgtgc cagtatggat gcaatgagag atttacccac
     1141 accgcctttg ccttgcaata tcatgtgtac tttagccatt agcataacct cttcataaga
     1201 taagttgaat ttatagtgca gctttttaga tcacgtggta aatctttatg ggtactgcgg
     1261 gaacgataat attgatgcgg taactttggt ttttatcgta tctatcagat tatatcgtca
     1321 acgttataat cagcactcca ttcaaaacct ttcggatttc cggtatcagg atatttaggg
     1381 gacttaggag aatctgtctt ttcttgtgcg ggctctaaat cttttttatt ctcactaagg
     1441 ctttccttat gggtatcgtc ggaacgatta ttttgttttt cttctacctc actttctagt
     1501 tcactttttt ttgtacttct tttatgcgat ttgtaatccg tgtgtttttt aactaaccgt
     1561 aaaaacacct gataactgat taccatcttt ccttcatcgt gcaata
//
LOCUS       DAORVT010000273         1575 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_93787_length_1575_cov_4.550658, whole genome shotgun sequence.
ACCESSION   DAORVT010000273 DAORVT010000000
VERSION     DAORVT010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93787_length_1575_cov_4.550658"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /gene="ndk"
                     /locus_tag="PK389_05550"
     CDS             complement(<1..410)
                     /gene="ndk"
                     /locus_tag="PK389_05550"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018574538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HQY23196.1"
                     /translation="MTVERTLSIIKPDAVAKNVIGAIIGRFERANLKVIAAKMLHLTQ
                     ERAEAFYAVHKDRPFFNDLVKFMISGSVFVQVLEGDNAILKNREIMGATNPSDAAKGT
                     IRADFADSIDANAVHGSDSSDTAKVEIAFFFTEQ"
     gene            532..>1575
                     /locus_tag="PK389_05555"
     CDS             532..>1575
                     /locus_tag="PK389_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HQY23197.1"
                     /translation="MSLNSFNSRSTFEHAEKTVTFYDLNKLPKSCDIGRLPYCLKILL
                     ENLLRCEDGISVTQKDIEALLNWAPHTPSDHEIAFTPARVILQDFTGVPAVVDLAAMR
                     DAMCQLGGKASLINPLSPVELVIDHSVMVDFYGKLDSFEKNVDMEFNRNEERYAFLRW
                     GQTAFQNFRVVPPGTGIVHQVNLEYLARVVFEQQQDGTSIAYPDTLVGTDSHTTMING
                     LGILGWGVGGIEAEAAMLGQPVTMLIPEVIGFKITGKLPEGTTATDLVLTVTEMLRKK
                     NVVGKFVEFYGDGLDHLPLADRATIGNMAPEYGATCGIFPIDTETLAFMRLCGRAESL
                     IKLVETYAKTQGLF"
BASE COUNT          457 a          354 c          310 g          454 t
ORIGIN      
        1 tcttgttctg taaagaaaaa agcgatttcc actttagcgg tatcactact gtctgaacca
       61 tgcaccgcat tcgcatcaat gctatcggca aaatccgccc ggatagtgcc tttagccgca
      121 tcactggggt ttgttgcacc cataatctca cgatttttaa gaatcgcatt gtcgccttct
      181 aacacttgaa caaaaacaga tcccgaaatc ataaatttaa ccaagtcatt aaaaaatggc
      241 ctatctttat gcacagcata aaaagcttct gcacgctctt gcgttaaatg cagcattttt
      301 gccgcaataa cctttaggtt agcgcgctca aaacggccaa taattgcgcc tatcacattt
      361 ttagcaacgg catcgggttt aataatcgaa agtgtacgtt caacggtcat aatagagtta
      421 ctccaccaac ttataaatac tgtctgaaag cctattatac cttaacctat agggaacttg
      481 aaggagatag tctaaacttt atatttgaat atcgcggtat ggagcaacac aatgtcttta
      541 aatagtttta attctaggtc aacattcgaa cacgcggaaa aaacagttac tttttatgat
      601 ttaaacaaac tccccaaatc ctgtgatatt ggtcgtctcc cctattgttt aaaaattctt
      661 cttgaaaatt tattgcgatg cgaagatggg atctccgtca ctcaaaaaga cattgaagcg
      721 ctattaaact gggcacccca tacgccttct gatcatgaaa tcgcctttac acccgcacgc
      781 gttattctac aagactttac gggtgttcct gccgttgtcg atttggctgc tatgcgcgat
      841 gccatgtgcc agcttggagg caaagcgtca ttgatcaatc cgctttctcc agtcgagtta
      901 gtcattgatc attccgtcat ggtcgatttt tacggcaaat tagattcttt cgaaaaaaat
      961 gtcgatatgg aatttaatcg aaatgaagaa cgctatgctt ttttacgttg gggacaaacc
     1021 gcttttcaaa attttagagt cgtacctccc ggtacaggta tcgttcacca agttaatctg
     1081 gagtatctcg cacgcgttgt ctttgaacaa caacaagacg gtacttcgat tgcttatcca
     1141 gacaccttgg tgggtaccga ttcgcatacc accatgatta atggattagg catattagga
     1201 tggggtgttg gaggtattga agcagaagcc gccatgctcg gtcagcccgt taccatgctg
     1261 atcccagaag ttattggttt taaaataacc ggaaaattac cagaaggcac aaccgcaacc
     1321 gatctggttt tgacggtcac cgaaatgctt cgcaaaaaaa atgtcgttgg aaaatttgta
     1381 gaattttatg gcgatggttt agatcattta ccactcgccg atcgcgcaac cattggcaac
     1441 atggcacctg aatatggcgc aacctgcggc atctttccca ttgatacaga aacactcgca
     1501 tttatgcgac tgtgcggtcg agcggagtcc ctcataaaat tagttgaaac ttacgcaaaa
     1561 acccaaggtt tattt
//
LOCUS       DAORVT010000274         1550 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_96526_length_1550_cov_2.819398, whole genome shotgun sequence.
ACCESSION   DAORVT010000274 DAORVT010000000
VERSION     DAORVT010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96526_length_1550_cov_2.819398"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PK389_05560"
     CDS             complement(<1..402)
                     /locus_tag="PK389_05560"
                     /inference="COORDINATES: protein motif:HMM:NF015354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HQY23198.1"
                     /translation="MIKRSAGQSSVASAAYRRGTILRDERTGLVHNFSYKTNVIYSEI
                     FVPDNAPLWITEICELQKTDPNRAGEALWNSVEKKEKRVDSQLSREFEFALPLELNKN
                     ECIELTKAYVKTLTDQGMVVDVSIHWDKGNPH"
     gene            671..976
                     /locus_tag="PK389_05565"
     CDS             671..976
                     /locus_tag="PK389_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HQY23199.1"
                     /translation="MNNPTLEKLVNRKERLEARVKLIKYRTMNQERKNDTRRKILAGA
                     YILQQCERDNRLTAFIQELTQFLTRDADKALFKLPFKSITPTVPAPTQTRAEAEPCN"
     gene            967..>1550
                     /locus_tag="PK389_05570"
     CDS             967..>1550
                     /locus_tag="PK389_05570"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HQY23200.1"
                     /translation="MQLMIVDSYRKKKALQAYCGFDWRIVVCAGQICDLPTYSLGIAE
                     PDFKPRYELLENATPFMKSLNQLAATADHVWLAMESNREGEILARHLAQHLIADSIKR
                     IVFSHKTKDGITNALNNPRKIDQQLCDAYVARRVIDRLIGYKVSNQLTQMFQKPVAVG
                     RVQSIALKLIVKREQALRAFRTVQCSIDSSNEPI"
BASE COUNT          439 a          312 c          319 g          480 t
ORIGIN      
        1 gtgtgggtta cccttatccc agtgaatact cacatcgaca accatccctt ggtcggttaa
       61 tgtttttaca taggcttttg ttaattcaat acattcgttt ttattcaatt ctagtgggag
      121 tgcaaattca aattctcgcg acaattgcga atctactctt ttttcttttt tttctacaga
      181 gttccaaagt gcttcgccag cacgattggg atctgttttt tgaagttcgc aaatttccgt
      241 aatccataac ggcgcgttat caggtacgaa aatctcgcta tagataacat tggttttata
      301 tgagaaatta tgcacaagac ccgtgcgctc atctcgtaaa atggttcctc ttctgtaagc
      361 agcggaggct accgaacttt gcccggcgct tcgtttaatc attttgactg gtaaatgata
      421 gatagccatg ttcggacaat ataatgcacg atgttgcgaa gcaacatata agtgcgccct
      481 cactatcgtt cggtgctgtc gcttcgataa gtgccatttt tatcccctgc gttaatttgt
      541 ttgtttgttt tttggcacac ccttttggat aggtgaatac ggattttgaa tgacaaattt
      601 gtccctgttg caactctttt ttgtaggcct cattctgatt ttaagtaaga atttttttgg
      661 agtatggaac atgaacaatc caacattaga aaagctggtt aatcgaaaag aacgattaga
      721 ggctagggtt aaattgatta aatatcggac aatgaatcaa gaacggaaaa acgatacgcg
      781 gcgtaaaatt ttggcgggtg cttatatttt acagcaatgt gagcgtgata atcggctcac
      841 ggcctttatc caagagctta cccaattttt gaccagagat gctgataaag ctttattcaa
      901 gttgccgttt aaatcaatca cccccactgt tcctgctccc actcaaaccc gtgcggaggc
      961 tgaaccatgc aattaatgat tgtagattct taccgcaaga aaaaagcgtt acaagcctac
     1021 tgtggttttg attggcggat cgttgtatgt gcaggtcaaa tttgtgactt gccgacttat
     1081 tcgttaggaa ttgctgaacc cgattttaaa ccccgctatg aattgttgga aaacgctacc
     1141 ccttttatga aatcgttaaa tcaattggca gcaacggcgg atcatgtatg gttagccatg
     1201 gagtctaatc gcgagggaga aatattagcg cgtcatttag cccaacatct gattgcggat
     1261 tccataaaac gtattgtatt tagtcataaa accaaagacg gcattacaaa tgccttaaat
     1321 aatcccagga aaattgatca acaactgtgt gatgcctatg tagccaggcg cgtcattgat
     1381 cgcttgattg gatacaaagt atctaatcaa ttaacccaaa tgttccaaaa gcccgttgca
     1441 gtaggtaggg tacaatccat tgcgcttaaa ttaatcgtga aacgcgaaca ggcattgcgt
     1501 gcatttagaa cggtgcaatg ttccatcgat tcaagcaatg aaccaatatt
//
LOCUS       DAORVT010000275         1544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_97199_length_1544_cov_3.146407, whole genome shotgun sequence.
ACCESSION   DAORVT010000275 DAORVT010000000
VERSION     DAORVT010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97199_length_1544_cov_3.146407"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PK389_05575"
     CDS             complement(<1..214)
                     /locus_tag="PK389_05575"
                     /inference="COORDINATES: protein motif:HMM:NF013934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HQY23201.1"
                     /translation="MMAENQNTKAAVRRSIRLERAKLSIKQQAKKAEQLMQIIVQLPE
                     FIHSQHLAAYWPNDSEINPLGILATAH"
     gene            complement(252..437)
                     /gene="ssrS"
                     /locus_tag="PK389_05580"
     ncRNA           complement(252..437)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PK389_05580"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(449..778)
                     /locus_tag="PK389_05585"
     CDS             complement(449..778)
                     /locus_tag="PK389_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010321565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HQY23202.1"
                     /translation="MDKSKSVTVTLLNKEFQVSCPANAEDQLNDAARYLNDKMREIRK
                     SGRVIGMERIAVMAALNIAYELAVLRQQQEAYLKSVSTQIEHLQNKIDNALMDELIEL
                     EGSQDSV"
     gene            927..>1544
                     /locus_tag="PK389_05590"
     CDS             927..>1544
                     /locus_tag="PK389_05590"
                     /inference="COORDINATES: protein motif:HMM:NF017046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HQY23203.1"
                     /translation="MSTMLKLMGITLKEYAARRSRIIENMPPKSVAILPGGSQKYRNA
                     DTEYPFRQESDFYYLTGFCEPKSLLVLLKKSASETQFILFCQSNNPELEIWTGKKVGL
                     DGALEQFCADKAYLFDDLDRIMPDLLENINEIFYSLGRHSDWDERVTGWLNSVCVQKK
                     QGVKAPGVFKDLSCFIHEARLFKSPDEIKLMKKASEISANAHVALI"
BASE COUNT          458 a          290 c          334 g          462 t
ORIGIN      
        1 catgggcggt agccaaaatg ccgaggggat ttatttcgct atcatttggc cagtaggctg
       61 caaggtgttg actgtgaata aattctggca attgaacaat gatctgcata agctgctcgg
      121 ctttttttgc ttgttgtttt attgatagct tggccctctc taaacgaata gaacgtctaa
      181 cagctgcttt ggtattttga ttttctgcca tcatgataat aaaaatcagc taaagcggcc
      241 tcagaaaaag gcggcgccct caaaagtgcc gctgtacagc gtttcccttg aaccgtaaag
      301 ttcaagtggg agtcaatgtg ccgtatcagg ctttccacac aaggcagaca tgcacaacaa
      361 cgacaacagg aagaccccct gtcagttaat tatcggctca agaagtaaga ctgtactggc
      421 gagcacccga gaaaggcgcc aatgaacgtt aaactgaatc ctgagatcct tcgagctcga
      481 tcagttcatc cattaaagcg ttatctattt tattttgcaa gtgttctatt tgtgtagata
      541 ctgatttgag gtaggcctct tgttgttgtc ttaagactgc taattcataa gcgatgttta
      601 aagccgccat aacagcgatc cgctccatcc caatgacacg gccacttttg cgaatttcgc
      661 gcattttgtc gtttaaatag cgtgcagcat catttagctg gtcttccgcg tttgctgggc
      721 aagagacttg aaattcctta tttaagagcg taacggtaac agatttagat ttatccacaa
      781 tttatctctc acaggcgcta tgattgctat aagaatagag tgactttagg agcgggtcaa
      841 tcagataatg taggtcctag tcgtattaat ccaggaataa gtaagtttta tttcaaaaac
      901 agagagatct taaaggatga gatatcatgt caacgatgct taagttaatg ggtattactc
      961 ttaaagaata tgctgcacgt cgcagccgta tcatcgaaaa tatgccgccg aagagcgtcg
     1021 cgattttgcc gggtggttct caaaagtacc gaaatgcaga taccgaatat ccttttcgtc
     1081 aagaaagtga tttttattat ctgaccggtt tttgcgaacc aaaatcgtta ttggtattgt
     1141 taaaaaaatc agcgagcgaa acacaattta ttttgttttg tcaatcaaat aatccagaac
     1201 tagaaatttg gactggcaaa aaagtgggtt tggatggagc actagaacaa ttttgtgcgg
     1261 ataaagccta tctttttgat gatttagatc gaataatgcc ggatttgtta gaaaatataa
     1321 acgagatttt ttattcctta ggacgtcatt ctgattggga cgaacgtgtc acaggttggt
     1381 taaacagtgt ttgtgttcaa aaaaaacaag gcgttaaagc gcctggcgtt ttcaaagatt
     1441 tatcttgttt tattcacgag gcgcgtctct ttaaaagccc tgatgaaata aagttaatga
     1501 aaaaagcatc agaaatttca gccaacgcac atgttgcatt aata
//
LOCUS       DAORVT010000276         1538 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_97829_length_1538_cov_4.721510, whole genome shotgun sequence.
ACCESSION   DAORVT010000276 DAORVT010000000
VERSION     DAORVT010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97829_length_1538_cov_4.721510"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..1319)
                     /locus_tag="PK389_05595"
     CDS             complement(204..1319)
                     /locus_tag="PK389_05595"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HQY23204.1"
                     /translation="MQENVIYVGVDVDDNSFHVTALMPETEEVIETKTKPNLKGLVNK
                     FEDFKKKFPNHALKICYEATYIGFTLQRDLASKGYDCVVVAPSSIPRVHGNQVKTDRV
                     DAGKLAQFLSAGILTFVSVPDKETESDRDLLRSRQFMLHQLAEVRTHIQSLLRRNGIH
                     YKSETKNISHWTKHHLSWLDTKADEAVGSFKQNLKLLVQQMKWLNHSLSEYGKAVDEL
                     ATTEKYKKQVSALVCYKGIKNIFAMVMITEIGNVKRFAHPRQLVSWMGMDVREYSSGG
                     KSHRFGITGHGNRYLRTAFVEANQRAIGGTRLSDDLKQRRKNIDPDFIHIADRCMARI
                     RKKGMRLLHAGKHSNKVKVACAREMVGFVWESLNKVA"
BASE COUNT          437 a          335 c          264 g          502 t
ORIGIN      
        1 tgaactaaca tcctcgtatt cgcgtgctgg ataacgcatt aagatttgtt cgttatatgg
       61 ggttaccaca acctagacac agacacttta atctaaagcc ggacccgtgg acttcccaaa
      121 ttctgtctta ccctctcatc aaatcctgat tttgcaggac tttaaaaaac tactttctaa
      181 ctaactaaaa aatatcaaaa tctttacgca actttattca acgattccca aacaaagccc
      241 accatttctc tggcacaggc aactttcact ttatttgaat gttttccagc gtgcagtagg
      301 cgcatgcctt ttttccggat tctagccatg catcgatctg caatatgaat aaaatctgga
      361 tcaatatttt ttcgcctttg tttcaaatca tcacttaatc ttgttccacc aattgctctt
      421 tgattggctt caacaaaagc tgttcttaaa tatcgatttc catgccctgt aattccaaac
      481 ctgtggcttt taccgccaga agaatactca cgaacatcca ttcccatcca ggacaccagt
      541 tggcggggat gggcaaatct tttaacattg ccaatttcag ttatcattac catagcaaaa
      601 atatttttaa tccctttgta gcaaactaat gcactcactt gttttttata tttttcagtc
      661 gtagctaatt catctacagc ttttccatac tcagataaag agtggtttaa ccacttcatt
      721 tgctgcacta aaagttttaa attttgttta aagcttccta ctgcttcgtc ggctttcgta
      781 tcgagccaac tcaggtggtg ctttgtccaa tgggaaatat ttttcgtttc tgatttataa
      841 tggattccat ttctacgaag aagagattga atgtgggttc tgacttcggc aagctgatgc
      901 agcatgaact gacgggacct gagcaaatct cgatctgact cagtttcttt atccggtaca
      961 gatacaaatg ttaaaattcc tgctgataaa aattgagcaa gcttgccagc atctacacga
     1021 tctgttttta cctggtttcc atgaactcta gggatactgc ttggagcaac aacaacgcag
     1081 tcgtagcctt tgctagcaag gtccctttgt agggtaaagc caatatatgt ggcctcgtaa
     1141 cagatcttta atgcatgatt tggaaatttc tttttaaaat cttcaaattt attaacaaga
     1201 ccctttaaat taggcttggt tttagtttca atgacttcct cagtttctgg cataagcgca
     1261 gtgacatgga atgaattatc gtcgacatcg acacctacat atattacgtt ttcttgcata
     1321 gacggctccc ttcgtatttg tttggtagct taattaaagc tccaaatgag gaatccatca
     1381 aacgatggta gccctcggtg ttaagaaggg ccgtctatat cttctagacc taaaattctg
     1441 ctttggttaa gaaatacaaa gatgagccgc gccgttttgt acaaacggaa aaagacaaat
     1501 taattgttga gtatgcgccc ctaattaaat tcatcgca
//
LOCUS       DAORVT010000277         1538 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_97858_length_1538_cov_3.061362, whole genome shotgun sequence.
ACCESSION   DAORVT010000277 DAORVT010000000
VERSION     DAORVT010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97858_length_1538_cov_3.061362"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1275
                     /locus_tag="PK389_05600"
     CDS             <1..1275
                     /locus_tag="PK389_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017521123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavohemoglobin expression-modulating QEGLA
                     motif protein"
                     /protein_id="HQY23205.1"
                     /translation="KYHQVIRELSEKIVAAQRPIRILDALKWNSTVEEAFFKSGCKEL
                     PNVRGPEFYLQNPLKFDPDKKIDEFNQIERDIRRELGQFNSVGSIMQRMCREYCTVVE
                     MLNARGTPHFTRYAQQLYGSADDAFYAGAPTLTDLAILISNTLSFIEKKTGSELDQKK
                     YTGDEAVSILGQRLSKYFEGEKEKTCVKLSDDIIADAAAGAEFIKLRKNAMFSERDLR
                     VLEVHEGWVHLGTTLNGMSQPICTFLSKGPPSSTITQEGLAIIMEIFTFSSHPARIQG
                     LTDRIRAINMAEKGGNFLDVFNFFREQGYSEAESYTRTARVFRGSTAESGPFTKDLVY
                     NKGFILIYNYIRLAIQQGLLSRIPLLFLGKATLEDLHILNDLVEEGIVAPPKYVPPQF
                     KDLAALSSWMCYSLFLNQLDLKRLAQEYKEIL"
BASE COUNT          493 a          266 c          333 g          446 t
ORIGIN      
        1 aaataccatc aggtgattcg tgaactctca gagaagattg tagctgcaca gcggccgatc
       61 cgcattttag atgctctaaa atggaatagt accgttgaag aagccttttt taaaagtggt
      121 tgcaaagaac tgcctaatgt acgtgggcca gaattttacc tccaaaatcc tcttaagttt
      181 gatccggata aaaaaattga tgagtttaat caaattgaac gcgatattcg acgtgagcta
      241 gggcaattta acagcgttgg atctattatg cagcggatgt gtcgcgaata ttgcactgtt
      301 gtcgaaatgt taaatgcgcg tggaacacct cattttacgc gctatgcaca acagctgtac
      361 ggaagtgcag atgatgcatt ttatgcggga gcgcccacgt taaccgatct tgcgatcttg
      421 atttcgaata ctttgtcttt tattgaaaaa aaaacgggct cagaattaga tcaaaaaaaa
      481 tatacaggtg acgaagccgt tagcatttta gggcaacgat tatcgaagta ttttgagggt
      541 gaaaaagaga aaacttgcgt taaactaagt gacgatatta ttgccgatgc agcagcaggc
      601 gcggagttta taaagttaag aaaaaatgcg atgtttagtg aaagagattt gcgtgtatta
      661 gaagtgcacg agggctgggt tcatttggga acgacattaa atggcatgag tcaaccgatt
      721 tgtacttttt taagtaaagg accaccttct tcaacgatta cgcaagaagg tttagcaatt
      781 attatggaaa tttttacttt ttcctcacat cccgcgagaa tacaaggttt aaccgaccgt
      841 attcgtgcga ttaacatggc ggaaaagggt ggaaattttt tagatgtttt taattttttt
      901 cgtgaacaag ggtactcaga agcagaaagt tatacacgaa cggcacgcgt gtttagaggt
      961 agtacagccg agagtggtcc ttttacaaaa gatttggtgt ataacaaagg ctttattctt
     1021 atttataatt atattcgact ggctattcaa caaggtttat tgtcgcggat ccctttactt
     1081 tttttaggta aagcaacgtt ggaagattta catattttaa atgatttggt ggaagaaggt
     1141 attgtcgctc cacctaaata tgtgccccca caatttaaag atttagccgc cttaagttcg
     1201 tggatgtgtt attccttatt tttaaatcaa ctcgatctaa aacgattagc ccaagaatat
     1261 aaagagattt tgtagtccat caaaaagggc ttaaaagaag aatagcttaa gacaaaaatg
     1321 ctcgcaaaaa tcgtagcgac accaagccag agagattttt caagtggcag ttcttgaaac
     1381 gccagatatc ccaccaaaac attgataaaa aatttgctaa atccaatagg cgttaaaaag
     1441 gttacttccg ccagttgata ggcttttgca aactataaat gtgcgccagc cgccaataca
     1501 cctaacaaga ttaaccaagg ccagtgagtt atgcttgg
//
LOCUS       DAORVT010000278         1536 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_98077_length_1536_cov_3.851452, whole genome shotgun sequence.
ACCESSION   DAORVT010000278 DAORVT010000000
VERSION     DAORVT010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98077_length_1536_cov_3.851452"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /gene="rpoB"
                     /locus_tag="PK389_05605"
     CDS             complement(<1..815)
                     /gene="rpoB"
                     /locus_tag="PK389_05605"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890497.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HQY23206.1"
                     /translation="MSGTDALELSYTERKRFRANYGKRRDTLKVPPLLNIQKQSYHAF
                     LQPDVLPLARKNTGLHAAFSSVFPIISYAGHAILEYVSYQLGTPAFDVKECQMRGLTY
                     AAPIRVTVRLVIYDKESPIDAKVIKDIREQDVYMGELPLMTNSGTFVINGTERVVVSQ
                     LHRSPGVFFEHDKGKTHSSGKLLYAARIIPYRGSWLDVEFDPKDCIYVRIDRRRKLPG
                     SIILRALGYSTEEMLALFFETNTFTFEKEKWVLELVANRLRGETAHFDIKTPKG"
     gene            complement(967..1344)
                     /gene="rplL"
                     /locus_tag="PK389_05610"
     CDS             complement(967..1344)
                     /gene="rplL"
                     /locus_tag="PK389_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004916934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HQY23207.1"
                     /translation="MALSNAEILKAVAQKSVMDIVELIEMMEKEFNVTAAVAVAAAPA
                     AGGAGAAAVEEQTEFNVVMTNHGEKKVEVIKAVRTITGLGLKEAKDLVEGTPAIIKEA
                     VSKQDAEAVKKQLVEAGATVEIK"
     gene            complement(1379..>1536)
                     /locus_tag="PK389_05615"
     CDS             complement(1379..>1536)
                     /locus_tag="PK389_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007148782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HQY23208.1"
                     /translation="LALVASLPTRSEAIAKLLAVMQAPIAKFVQTLAAPTSKFVRTLA
                     AVRDQKQ"
BASE COUNT          440 a          366 c          282 g          448 t
ORIGIN      
        1 cccttcggtg ttttgatatc aaaatgcgca gtctcgcctc ttaaccgatt agccaccaac
       61 tctaaaaccc atttttcttt ttcaaatgta aaagtattgg tttcaaagaa aagcgctaac
      121 atctcctctg tagaatagcc taaagcacgt aatataatgg atcccggcag ttttcgacgc
      181 ctatcaatac gaacataaat acaatcttta ggatcaaact caacgtcaag ccaagatcct
      241 cgatagggaa taatacgagc agcatataac aacttacccg acgaatgcgt tttgccttta
      301 tcatgttcaa aaaagacacc tggagaacga tgcaattgtg atacaacgac tcgctcggta
      361 ccattgatca cgaaggttcc actgtttgtc atgagtggaa gttcgcccat atagacatct
      421 tgttctcgaa tatctttgat tacttttgca tcgattggcg attctttatc gtagataacg
      481 agtcgtaccg taacgcgtat cggcgctgcg tacgttaaac ctcgcatttg gcattcttta
      541 acatcaaaag ctggcgttcc taattgatag cttacatatt ctaagatcgc gtgaccggca
      601 tagctaataa tgggaaatac cgaactaaat gcagcatgca atcctgtatt tttccgcgct
      661 aacggcaaaa cgtcaggttg taaaaatgca tgatacgact gcttttggat gtttaataag
      721 ggtggaactt ttaacgtatc tcgacgctta ccatagttag cacgaaaacg ctttcgctca
      781 gtataagaaa gttctaatgc gtcagtaccg gacatattga ttcctctgac aaatattaaa
      841 aataaccaaa acggctagca gacgtagggg cggacgaagg gaggacacgg cagtccgccc
      901 ctacagccca tttatcagct gctaaccatt cccacattag gccggcgttg aatccgacct
      961 tgccatttat ttaatttcaa ccgtagcgcc ggcttcaacc aactgcttct taacagcttc
     1021 agcatcttgc ttagatacag cttctttaat aatcgcaggt gtgccttcga ctaaatcttt
     1081 agcttctttt aagcctaaac ctgtaatcgt tcgcacagct ttaataactt ctaccttctt
     1141 ttcgccatga tttgtcatca caacgttaaa ttcggtttgt tcttcaacag cagcagcacc
     1201 cgcgccaccc gctgctggag ctgcggccac tgcaacagct gccgttacat taaattcctt
     1261 ttccatcatt tcaattaatt caacaatatc cattacgctc ttttgagcga cggcttttaa
     1321 aatttctgca ttcgataagg ccattttatt tctccacaca ctatataaag gttaaaaatt
     1381 actgtttttg atcacgaaca gccgccaaag ttcttacaaa cttagacgta ggtgctgcca
     1441 aggtttgtac aaatttcgca ataggcgctt gcatcactgc caatagtttg gcaatggctt
     1501 cagaacgtgt tggtaaactt gcgactaaag ctaact
//
LOCUS       DAORVT010000279         1535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_98229_length_1535_cov_2.704054, whole genome shotgun sequence.
ACCESSION   DAORVT010000279 DAORVT010000000
VERSION     DAORVT010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98229_length_1535_cov_2.704054"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PK389_05620"
     CDS             <1..391
                     /locus_tag="PK389_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23209.1"
                     /translation="QFLAFQFEYEILSDDYLITVLKYYVDNARTVDPAKLQEMLEEEL
                     RGFKKGEVMATIASHLTQKGRQEGRQECGASLLMNMLKVKFNDLSDAYIEKINRADVN
                     TLNQWCINFVNAKSLDQVFTNTSNIMG"
     gene            complement(350..1135)
                     /locus_tag="PK389_05625"
     CDS             complement(350..1135)
                     /locus_tag="PK389_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019528406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQY23210.1"
                     /translation="MQQKRLGNTDITVSVLGLGTVKFGRNQAVKYPEAFELPDDQHLS
                     HLLNSARDLGINLIDTAPAYGNSEARLGTLLSDSRKNWVIVSKVGEEFVNGNSIYNFT
                     PEHAEKSIKRSLKQLNTDYIDVVLVHSDGNDVYNIEHFGILHFLSELKKQGLIRAFGM
                     STKTVEGGIRALEQSDVVMVTYNPTETTEQPVIAKAFELNKGVLIKKALASGHIQKIP
                     GLDPVKTSLQFILQEPGVSSIILGTLDPAHLAHNVACISKNLI"
     regulatory      1239..1357
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1347..>1535
                     /locus_tag="PK389_05630"
     CDS             1347..>1535
                     /locus_tag="PK389_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012051544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23211.1"
                     /translation="MSTHLELVNAKQKMDLASLKAFFPAVFGTIVEYYDYALYGFCAS
                     LLASQFFPTDDPTVALLKT"
BASE COUNT          460 a          293 c          317 g          465 t
ORIGIN      
        1 acagtttttg gcattccagt ttgaatatga aattttgagt gacgattatt taataacagt
       61 gctaaaatac tacgtggata atgcacgaac cgtcgatccc gctaagcttc aagaaatgct
      121 tgaagaagag ttaagaggtt ttaaaaaagg agaagttatg gcaacaatag caagtcatct
      181 tacgcaaaaa ggccgacaag aaggtcggca agaatgtggt gcatctttgt taatgaatat
      241 gttaaaagtt aagtttaatg atttgtcgga tgcgtatatt gaaaaaataa accgtgctga
      301 cgtcaatacg ctgaatcagt ggtgcattaa tttcgtgaat gcgaaatcgt tagatcaggt
      361 ttttactaat acaagcaaca ttatgggcta aatgtgcggg atcgagcgtt cccaaaataa
      421 tactgctaac acccggttcc tgaagaataa attgtaacga agttttaacg ggatctaaac
      481 ctgggatttt ttgaatatgc ccgctggcaa gcgctttttt aataagcaca cctttattta
      541 attcaaaggc ttttgcaata accggttgtt cggttgtttc cgtaggattg taagtaacca
      601 ttaccacatc ggattgttcg agtgctcgaa tgccaccttc aaccgttttg gtagacatac
      661 caaaagcacg gatcagacct tgttttttta attccgatag aaaatgcaaa atgccaaaat
      721 gctcaatatt ataaacatca ttgccatcag aatgcacaag gacgacatca atataatccg
      781 tattcaattg ttttagactg cgtttaatgc ttttttccgc atgttctggc gtaaaattat
      841 agattgaatt tccgtttaca aattcttctc caactttgga tacaataacc cagttttttc
      901 tggagtccga taataaggta cctaatcgtg cctcactatt cccataagcc ggagcagtgt
      961 cgattaagtt aatgcctaaa tcgcgtgcgg aattaagaag gtgagacaga tgttggtcgt
     1021 ctgggagctc aaaagcctct ggatatttaa cggcttgatt acgtccaaat ttaacggttc
     1081 ccaatccaag cactgaaact gttatgtctg tattacctag tcttttctgt tgcacattca
     1141 cacccaaagc gtatgggggc aaaccccgac aagaaatgat atcattcatc acgcgcgttt
     1201 agctatcgtt gacatcctat tcgtcttaat atagtttgga gattcaccag gggtgccggt
     1261 gcgaaatgtg acatcggctg aaaaaacgca tggggcgttt aacccttaag acctgatctg
     1321 ggtaatacca gcggagggag cgtggcatgt caacacatct tgaattagtc aatgcaaaac
     1381 aaaaaatgga tttagcgtct cttaaagcgt tttttcctgc cgttttcggc actattgttg
     1441 aatattatga ttatgcgctt tatggatttt gtgcaagtct tttagcctcg caattttttc
     1501 caacagatga tccaacggtt gcgttgctca aaacc
//
LOCUS       DAORVT010000280         1531 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_98653_length_1531_cov_5.048780, whole genome shotgun sequence.
ACCESSION   DAORVT010000280 DAORVT010000000
VERSION     DAORVT010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98653_length_1531_cov_5.048780"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..566)
                     /locus_tag="PK389_05635"
     CDS             complement(159..566)
                     /locus_tag="PK389_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23212.1"
                     /translation="MILLSGCAVSSENNHVPYISSAQFQTYSCNELLAEIERIQTRVS
                     QLTGKPDDNNPVKDKWVLGADLSLSWAALFALGGTKEQEAEYEQLKSEYDAIQQWAII
                     KQCPGVIPPAEKPPEFDPNLVETLKMDKVAGRR"
     gene            1056..1199
                     /locus_tag="PK389_05640"
     CDS             1056..1199
                     /locus_tag="PK389_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23213.1"
                     /translation="MRDHRANSCNIRARNNTDAMKIIKQLLLADMQKSNHYLFADPNG
                     QPK"
     gene            complement(1416..>1531)
                     /locus_tag="PK389_05645"
     CDS             complement(1416..>1531)
                     /locus_tag="PK389_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FxDxF family PEP-CTERM protein"
                     /protein_id="HQY23214.1"
                     /translation="TASYAVALSAVPEPETYAMLLAGLGLIGFSIRRRRMV"
BASE COUNT          455 a          339 c          273 g          464 t
ORIGIN      
        1 gcatcaaatc cgctaacaca ctgcccttgt aaaccacgcc cttgttggtg atcctcgcta
       61 tggcgaaaaa agttataatg ataagattgt aaaaatttat tttatttcct taaacaaatg
      121 ttctattctt gataatcacg ctattgcgca tagtttcgtt agcgccgccc tgccacttta
      181 tccatcttca atgtttctac caggtttgga tcaaattcag gaggtttctc ggccggcggg
      241 attacgcccg ggcattgttt gataattgcc cattgttgta ttgcgtcata ctcacttttt
      301 agttgctcat actcagcttc ttgttctttg gttcccccta atgcaaataa tgccgcccat
      361 gaaagcgata aatccgcacc gagcacccat ttgtctttga ctggattatt gtcgtccggt
      421 ttgcccgtca actgactgac tctggtctga atcctttcaa tctcagccaa taactcattg
      481 caactataag tttgaaattg tgcagatgaa atataaggca catgattatt ttctgaagat
      541 accgcgcaac ccgatagtaa aatcacagcg gaaaaaaaat agcagcaatt agtcttttca
      601 taatacctac ttccttcttg ggcaaggcaa atctattcta actctaattt tccagcagaa
      661 ttaccatatt acaaccacaa tataatttca tcagattgat atttaacgat tatttatttt
      721 tatttttcgt aaaaaataga gttaacaaga ttgcaagtca actcgttgtt aaataagaaa
      781 aaataccatt agaataaatc cgccttgcct tcttgggttt ttacattgac aagtaaagct
      841 tcctcacacc catttctgtg aattgtacac cgatattcgc actttcagtc ttacatcatg
      901 atgctgactg aaacgatcag aatcacagct agcccggaaa ttgcatgaat tgcacacgcc
      961 ctcacttgtc tcttgattcc acaagggatt tgtctcagcc aggtgtatga ataactacac
     1021 cgcttcttcg aaaaatccct tgtgaaacca atatcatgcg tgatcatcgt gcgaattcat
     1081 gcaatatccg ggctagaaat aatactgatg caatgaagat aattaaacaa ctgcttcttg
     1141 ctgatatgca aaagagcaac cattacttgt ttgctgaccc taatggacag ccaaagtaat
     1201 cagttaaaag acactcggac tggatgggtt tgaatttctg taggctatat cagaaggatt
     1261 ctagcggttt aactgacagg aaggatactt ttagctacca gctattcaat gcaccttttg
     1321 catttgctga tagaccgctt taacgtagtg aattcaggaa acaaaaaagc cacagagaag
     1381 tacttcttct ctgtggctat cgcgtcaagt tctgattaaa ccattctacg acggcgtatt
     1441 gaaaaaccga tcaaaccaag accagccaac aacatggcgt aggtttctgg ttccggaact
     1501 gcactaagtg caaccgcata tgatgcggtt g
//
LOCUS       DAORVT010000281         1529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_98945_length_1529_cov_2.633650, whole genome shotgun sequence.
ACCESSION   DAORVT010000281 DAORVT010000000
VERSION     DAORVT010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98945_length_1529_cov_2.633650"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="PK389_05650"
     CDS             <1..687
                     /locus_tag="PK389_05650"
                     /inference="COORDINATES: protein motif:HMM:NF020962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HQY23215.1"
                     /translation="ERKLLYRLNRSGLIFRLLRGVYLVPNHIPPGGFWRPNDYLIIAR
                     YMAYHKANYYIGGLTAFNKHGFSTQISNQLSVYNDKILGLKDFGGIKIKFYKIAKKYI
                     SGFDTLPIVSQVQTVNIANLAKTILDAINYNKRYQTLPEAYFWLQKYAHDKNFLKNFI
                     ELTYKTANNNATRRIGYFLEQLGVNKKTLLPLLEKLASTKGLVALVPNGSRQGRINKK
                     WGIIDNAQNR"
     gene            complement(699..>1529)
                     /locus_tag="PK389_05655"
     CDS             complement(699..>1529)
                     /locus_tag="PK389_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HQY23216.1"
                     /translation="TSVRFQTEMGVRIDRNTHCKQSLSAIESALVGNYRSEHLFSLKQ
                     ALEIYDFYAQKIKDCDLAIEKKLLEFSASVMQAEKATEPVKKKFSKNAPHFDLAQQMI
                     RVTGVDLSEVPGISSLTTLTLIGEIGLDMTRWKDAGHFASWMGLSPGNKVSGGKRLSG
                     KTKASANRAAAALRMATSTLHHSQTALGAYLRRLKSRRGAPKAITATAHKLATIIYHM
                     LRYGTRFKEMGQEYYEQQYKDRLLRNLKKKAEFLGFDLIEKVAIGNCSITAGNYTLIG
                     "
BASE COUNT          462 a          304 c          282 g          481 t
ORIGIN      
        1 gagcgcaaat tactctatag gcttaatcga agcggattga ttttccgctt actccgtggt
       61 gtctatcttg tgcctaatca tatacccccc ggaggctttt ggcgaccaaa tgactattta
      121 ataattgcaa gatatatggc gtatcataag gctaattatt acataggtgg tttaactgcg
      181 ttcaataaac atggcttttc cactcaaatt tcaaaccaat tatctgtata caacgataaa
      241 atattggggt taaaagactt tggtgggatt aaaattaaat tttataagat tgctaaaaaa
      301 tatataagtg gtttcgatac attaccgatt gtatcgcagg tgcaaaccgt taatatagca
      361 aatttagcta aaacgatttt agacgccatt aattacaata aacgctatca aacgttacca
      421 gaagcctatt tctggcttca aaaatacgct catgacaaaa attttttaaa aaactttatt
      481 gaacttacgt ataaaacagc taacaataat gcgactagaa gaattggtta ttttcttgaa
      541 cagttaggtg taaataaaaa aacacttttg ccgttactgg aaaaactagc atctactaaa
      601 ggcttggttg cattagtgcc gaatggttcg cgccagggga gaataaataa gaagtggggg
      661 attattgaca atgctcaaaa ccgttgatga cgagtcaatc acccaattaa ggtgtaattg
      721 cccgcggtta ttgagcagtt acctatggct actttttcaa taagatcaaa acccaaaaat
      781 tctgcttttt tctttaggtt tctgagcaag cgatctttat attgctgttc ataatattct
      841 tgccccatct ctttgaatcg agttccatac ctcaacatgt gataaatgat tgtagcgagt
      901 ttgtgtgcag tcgctgtaat agccttgggt gctcctcggc gagatttcag acgtcttaaa
      961 taagcgccta atgccgtttg gctatgatgt aaggtacttg ttgccattcg taaagcagct
     1021 gctgctcgat tggcacttgc tttggtttta ccacttaaac gtttaccgcc agaaaccttg
     1081 tttccaggag ataaacccat ccatgaagca aagtgacccg catctttcca acgtgtcatg
     1141 tctaatccaa tctccccaat aagcgtaagt gtagttaagc tactgatccc agggacttcg
     1201 ctcaaatcaa cccctgttac acggatcatc tgttgtgcga gatcaaaatg cggcgcattt
     1261 ttagaaaact ttttttttac cggctctgtt gctttttctg cttgcattac actagcagaa
     1321 aactctaaaa gctttttttc aatagccaga tcacagtctt ttattttctg tgcatagaaa
     1381 tcatagattt caagcgcttg ctttaaacta aataagtgct ctgaacgata attgccgacc
     1441 aatgcagact caattgcgct caaagattgt ttacaatgcg tattacggtc aattcgaaca
     1501 cccatttcag tctgaaagcg aacgcttgt
//
LOCUS       DAORVT010000282         1523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_99618_length_1523_cov_2.386921, whole genome shotgun sequence.
ACCESSION   DAORVT010000282 DAORVT010000000
VERSION     DAORVT010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99618_length_1523_cov_2.386921"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="PK389_05660"
     CDS             complement(<1..859)
                     /locus_tag="PK389_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005403892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HQY23217.1"
                     /translation="MINKIRPHSLPLYAWAAALTISILCCLSALGGGMLAWVSEADAQ
                     AIDTAGSIRMAAYQINFQLATNFAVNLPFDLNTDATNHQNKAISESNSINQPTSQKVT
                     ILIEDMENRLAKLDAYQLTNANDSQAIDSQLTQIHRQWLDSLKPALMSQDKQKFYIDS
                     IDYIKKVDRFVSELQHRNEKRQIWQQVLQIGSLIIIITILLIGMYKLRENVLLPVQDL
                     IKANSKFKQGDRQARAAVTGYAEFSALARSFNDMASIIATYQHSLESEVDIKTQHLVK
                     ANQVLSLFYN"
     gene            1220..>1523
                     /locus_tag="PK389_05665"
     CDS             1220..>1523
                     /locus_tag="PK389_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011279893.1"
                     /note="involved in the transport of nitrate and nitrite;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HQY23218.1"
                     /translation="MGTNSDFKGGKLITDWRPEVPEFWEEGGKKVARRNLWISIPALL
                     LAFAVWMVWSAVIVRLPEIGFKFDGGQLFWLAALPGLSGATLRIFYSFMVPIFGGRR"
BASE COUNT          424 a          271 c          324 g          504 t
ORIGIN      
        1 agttataaaa caatgataac acttggttag cttttaccaa gtgctgcgtt ttaatatcaa
       61 cttcgctctc tagggagtgc tggtaagtgg caatgatact ggccatgtca ttgaaagaac
      121 gagcaagtgc actaaattcg gcatagcccg tgacagctgc tctggcttgg cggtcacctt
      181 gtttaaattt actgttggct ttaatcaggt cttgcacagg tagaagtaca ttttcacgca
      241 gcttatacat gcctatcagc aaaatagtaa taattataat gagtgagcca atttgaagta
      301 cttgctgcca aatctgacgt ttttcatttc gatgctgaag ctcactgacg aaacgatcta
      361 cttttttaat ataatcaatt gaatcgatgt aaaatttttg tttatcttgc gacattaaag
      421 caggttttaa actatctaac cattgacggt gtatttgtgt tagctgacta tcaattgcct
      481 gactgtcatt agcgttagtt agttggtaag catctagttt tgccagtcta ttttccatgt
      541 cttcaataag aatagttact ttttgagacg ttggttgatt aatagagttt gattcactga
      601 tcgctttatt ttgatgattg gtagcatcag tgtttaagtc aaaaggaaga ttaactgcaa
      661 aattggtggc gagctgaaaa ttgatctggt aagcagccat gcgtattgag ccagctgtgt
      721 caatggcttg tgcatctgct tctgacaccc aagccagcat gccaccgcct aacgcagata
      781 aacagcataa aatagagatg gttaatgcgg cagcccaagc ataaagcggc aaagaatgtg
      841 gtcgtatttt atttatcatt gtgtcgggtg ctacttacca tgaataaact tttctctatc
      901 taaaaatttg aattgataaa caaataaaat tttaaaaaat ctgactgtaa gcttagctca
      961 aaaagcttag atcaagactt ttcatcgatt catctcgctc caatatactc ctttaagcga
     1021 tcaatgtacc actatgtatt aatactaata acactgataa caaattacct taagttaaca
     1081 ttttttgcta accccgctct tttttgagct aacaaacagg gaatcttgaa taggcgttta
     1141 aattatttag gcgtctgtgt tgtgatacct gttgtttatg cgggtttagc gctataagtg
     1201 attttaagtg aggaacagca tgggcacgaa tagcgatttt aaaggcggca agctcatcac
     1261 tgactggcgc cctgaagtac cagagttttg ggaggaaggc ggtaaaaaag ttgcgcgtcg
     1321 aaacttatgg atttctatcc ctgctttgtt acttgccttt gcggtatgga tggtgtggag
     1381 cgcggtgatt gtacgtcttc cagaaattgg ttttaagttt gatggcgggc aacttttttg
     1441 gttagctgca ttgccaggtt tatcaggtgc cacattacga attttttatt cgtttatggt
     1501 gcctattttc ggtggtcgtc gct
//
LOCUS       DAORVT010000283         1522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_99669_length_1522_cov_3.887526, whole genome shotgun sequence.
ACCESSION   DAORVT010000283 DAORVT010000000
VERSION     DAORVT010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99669_length_1522_cov_3.887526"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1497
                     /gene="pilQ"
                     /locus_tag="PK389_05670"
     CDS             <1..1497
                     /gene="pilQ"
                     /locus_tag="PK389_05670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HQY23219.1"
                     /translation="KYSGDKISLNFQDIEIRAILQIIADFSGFNIIASDSVKGNVTLR
                     LQSVPWDQALDIILKSEGLDKRQYGNVMLVGPAAEIASREKVELDSQKQMEELGSLKS
                     ELVQINYASAEDLSKLLNEKSNSLMTSRGTVSVDKRTNTLLIQDIPDRLDEIKALLKK
                     LDVAVRQVEISTQIVTADNTLASTLGMRFGGGANAGIGHRRLGVGSNIDRARAIGDFR
                     NGAGQSGMPPSNAAVSNANLGIIGTAGPNPGPTVSNTEGLFSDLAATSAGAPTATIGL
                     ALAKLPNGTLLDLELQAMEFESTSKTIARPNLITMDQNKASMEKGVEIPYQEASSSGA
                     TSVAFKTAALKLDVTPHITPDDKITLDLEISNDSKGDSISTGTSTIPAINTNRLKTKV
                     LVDNGETVALGGVLQVDDQKTVAKVPFFGDLPIIGGLFRNKFSQHKPRELIIFLTPRI
                     INPLLKNQRSRSSDYQSEKHDDCYVPCENKKVENSNYRISSENECKDR"
BASE COUNT          503 a          250 c          348 g          421 t
ORIGIN      
        1 aagtattcgg gagataaaat atctttaaat tttcaagaca ttgagatccg agcaatctta
       61 caaattattg cggatttttc ggggtttaat attattgcca gtgatagtgt taagggtaat
      121 gttacattac gtcttcaaag cgttccttgg gatcaagctt tagatattat tttgaaaagt
      181 gaagggttgg ataaaagaca atatggaaat gttatgctgg tagggcccgc agcagaaata
      241 gcctccagag aaaaagtcga gctagatagt caaaagcaaa tggaagaatt gggttctttg
      301 aaatctgaat tagtccaaat taattatgca agcgcagagg atttgtcaaa attattaaac
      361 gaaaaaagca attcattaat gacatcacgt ggaacggttt cagttgataa gcgaaccaat
      421 accttgttga tacaagatat tcccgatcgg ttggatgaga ttaaagcatt gttaaaaaag
      481 ttggatgttg cggttagaca agtagagatt tcaactcaaa ttgttaccgc ggataataca
      541 cttgcaagta cattaggtat gcggtttggt ggtggcgcta atgcagggat tggccatcgt
      601 cgcttggggg ttggcagtaa catagataga gcaagagcga taggtgattt taggaacggc
      661 gcagggcaat caggcatgcc accttctaat gcagctgtat cgaatgccaa tctggggatt
      721 ataggtacag ctggacctaa tccgggacca actgtcagta atacggaagg cttatttagt
      781 gatttagcgg caacatcggc aggggctcca acagcgacta taggcttagc tttagcaaaa
      841 ttgccgaacg gtacattgtt ggatttagag ttacaggcga tggaattcga aagcacaagc
      901 aaaacgattg caagaccaaa tctaatcacc atggatcaaa ataaagccag tatggaaaaa
      961 ggtgttgaga tcccttatca ggaagccagt tcaagtggtg ctacaagcgt cgccttcaaa
     1021 acagcagcgc taaaattaga cgtaacgccc catattacgc cagatgataa aattacctta
     1081 gatttagaga tttcaaatga ttctaaaggc gacagtattt caactggaac tagcactatt
     1141 ccagcgatta acacgaatcg attaaaaact aaagtgttgg ttgataatgg cgaaaccgtg
     1201 gcgttaggtg gtgtgttaca ggttgatgat caaaaaacag ttgctaaagt accgtttttt
     1261 ggagatttgc ctattatcgg tggtttattt agaaacaaat tttcgcaaca taaaccgaga
     1321 gagctaatta ttttcctaac acccagaatc attaatccac tgcttaaaaa tcaacgttcg
     1381 agatcatcag attatcaaag cgaaaagcat gatgattgct atgttccgtg tgagaacaaa
     1441 aaagtagaaa atagcaatta ccgtatttcg tctgaaaacg aatgtaaaga tcgttgattt
     1501 gattttttca tggtttgtcg tt
//
LOCUS       DAORVT010000284         1517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_100263_length_1517_cov_3.030780, whole genome shotgun
            sequence.
ACCESSION   DAORVT010000284 DAORVT010000000
VERSION     DAORVT010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100263_length_1517_cov_3.030780"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1287
                     /locus_tag="PK389_05675"
     CDS             <1..1287
                     /locus_tag="PK389_05675"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HQY23220.1"
                     /translation="ITFFVAAAICFFIPVALACAELSSGWPKEGGVYLWVEEAFGPHW
                     GFLAVWLQWMESVVWLPTILSFIAATAAYLFKPALESNKLFLVGVMLSVLWGTTLLNF
                     KDIKTSSFLSAIGVMLGTLIPGIVLIVLGCTQLANAEASGYLQFSLEALNPGTELSTL
                     VTFTAILLGLCGMEIPAYHVKNVQNPKKDYPTAMFLATLIILFIYILGSLSIAAVIPK
                     EKMSLIAGPMQAFHLFFNAFGLSWTAPLLAALTLIGSFAILNTWIIGPSKGLLSSTKD
                     GYMPKLFMRTNANESPVALLCLQGLCGTVLISLFVFNESIHAAYWIINTLAAQLYLIM
                     YFILFLAVIKLRYSQPNTPRAYKIPGGKLGVWFVGGLGAITCVTALLIGFAKPADIVF
                     QHTAMQYALLLLGGIVICCLPPVLFVRAHKKRNRKS"
     gene            complement(1298..>1517)
                     /locus_tag="PK389_05680"
     CDS             complement(1298..>1517)
                     /locus_tag="PK389_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008221881.1"
                     /note="molecular chaperone; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HQY23221.1"
                     /translation="SGQHVPTQKPILELNPDHRMIKHLKDEQDSARFEDWSRILLDQA
                     ILAEGGQLEDPASFVQRFNKLLLALTER"
BASE COUNT          376 a          331 c          322 g          488 t
ORIGIN      
        1 attactttct ttgtggctgc cgctatctgc ttttttatac ccgtcgcact tgcttgtgcg
       61 gagctttcgt cggggtggcc caaagagggt ggcgtatacc tctgggttga agaagccttt
      121 gggccccatt ggggattttt agccgtttgg ttgcaatgga tggagagtgt cgtttggctg
      181 ccaaccattt tatcgtttat tgcggcaaca gcagcttact tgtttaaacc cgcattagaa
      241 agcaataagc tctttttggt aggcgtgatg ttgagcgtgt tgtggggaac aacactactt
      301 aattttaaag atataaaaac atccagcttt ttaagtgcca tcggcgtgat gcttggtacc
      361 cttatcccag gtattgtact gattgtactt ggatgtaccc aattagcaaa tgccgaagca
      421 tccggctatt tacagttctc tttagaagct ctaaaccctg gaacagaact cagcacacta
      481 gtgactttta cagctatttt attaggactt tgcggtatgg aaatccccgc ttatcatgtc
      541 aaaaatgttc aaaatcctaa aaaagattac ccaaccgcta tgttcttggc aactttgatt
      601 atcttattta tctatatctt aggatcgctt tctatcgcag cagtcatccc aaaagagaaa
      661 atgagcctaa ttgccggccc catgcaagct tttcatttat tttttaatgc atttggactc
      721 agctggacgg cgccattact cgccgcacta accttaattg gcagctttgc gattttaaat
      781 acgtggatta ttgggcccag taaaggttta ttaagcagca ctaaagacgg ttatatgcca
      841 aaacttttta tgcgaaccaa tgcgaatgaa tcaccggtgg cgcttttatg tctgcagggg
      901 ctttgtggca ccgtcttgat ttctcttttt gtttttaacg agagcattca tgctgcctat
      961 tggattatta atacactagc agcacagctc tatttgatta tgtattttat tttatttttg
     1021 gctgtgatta aattacgtta cagtcaaccg aataccccaa gagcctataa aattcccggg
     1081 gggaaattgg gcgtttggtt tgtaggcggg cttggcgcca ttacctgtgt aacagcgcta
     1141 ctcattggct tcgcaaaacc tgccgatatt gtatttcagc acacagcaat gcaatacgcc
     1201 ctcctattac tcggtggcat tgtcatttgc tgtttaccgc ccgtcttatt tgtacgggcg
     1261 cataaaaagc gaaatcgcaa atcctaacag cctacgccta tctctccgtc aaagctaaca
     1321 acaatttgtt aaatctttgt acaaaactgg cgggatcttc cagttggcca ccctctgcca
     1381 aaatggcttg atccaataag atccgcgacc aatcttcgaa tcgtgcagaa tcttgttcgt
     1441 ctttcaagtg cttaatcatc ctgtgatcgg ggtttaattc taaaatgggt ttttgtgttg
     1501 gaacgtgttg tcctgaa
//
LOCUS       DAORVT010000285         1511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_100981_length_1511_cov_3.232143, whole genome shotgun
            sequence.
ACCESSION   DAORVT010000285 DAORVT010000000
VERSION     DAORVT010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100981_length_1511_cov_3.232143"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /gene="sdhA"
                     /locus_tag="PK389_05685"
     CDS             <1..451
                     /gene="sdhA"
                     /locus_tag="PK389_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011506516.1"
                     /note="part of four member succinate dehydrogenase enzyme
                     complex that forms a trimeric complex (trimer of
                     tetramers); SdhA/B are the catalytic subcomplex and can
                     exhibit succinate dehydrogenase activity in the absence of
                     SdhC/D which are the membrane components and form
                     cytochrome b556; SdhC binds ubiquinone; oxidizes succinate
                     to fumarate while reducing ubiquinone to ubiquinol;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HQY23222.1"
                     /translation="SALSEYNRWNTNKPGESVSDIRVELQKVMQNDFGVFRKEDVMQA
                     GIEKLKALRERLKFASLSDKSQTFNTARIEALELDNLMCVAIATAYAAVARKESRGAH
                     AREDYPTRNDTEWLKHTLYFSDDRIAYRPVNMKPISVDAFPPKARTY"
     gene            451..1149
                     /locus_tag="PK389_05690"
     CDS             451..1149
                     /locus_tag="PK389_05690"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HQY23223.1"
                     /translation="MKFSIYRFNPEVDKKPYMQDYELDLKETPAAMVLDALKLLKKQD
                     ETLSFRRSCGEGVCGSDGMNINGRNGLACVTPLAGIKEPVVLRPFPGMPVIRDLIVDL
                     TNFYKQYEKTKPYLQNSQPAPGIERLQSPAERAKLDGLYECIMCGCCSSSCPSYWWNP
                     EKFLGPAAALQAARFIEDSRDDATLERLAQLDDAYSVFRCRSIMNCVDVCPKNLNPTK
                     AIADIRDKIIKDGV"
     gene            1197..>1511
                     /locus_tag="PK389_05695"
     CDS             1197..>1511
                     /locus_tag="PK389_05695"
                     /inference="COORDINATES: protein motif:HMM:NF027404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23224.1"
                     /translation="MANYKVTMSELQKTALLDAGNSAYLEQLYDDYLKNPASVSTEWQ
                     QYFEKLPLVDGVVSETSHLDIQAAFKQMVRHPNCIKAGSIMPEASGGAVEIAQLKIAL
                     SRE"
BASE COUNT          482 a          276 c          347 g          406 t
ORIGIN      
        1 aagcgcttta agtgagtata accgatggaa tacgaataaa ccgggtgaaa gcgtttctga
       61 tattcgtgtt gaactgcaaa aagtgatgca gaatgatttt ggtgttttta gaaaagaaga
      121 tgttatgcaa gcaggtatcg aaaaattaaa agcccttcgg gagcgattaa agtttgcgag
      181 tctttcagat aaaagtcaaa catttaatac cgcgcgtatc gaagccttag aattagataa
      241 ccttatgtgt gtggcgatag caacggctta tgctgctgtt gcacgaaagg aaagtcgtgg
      301 tgcgcatgcc agagaagatt atccaacaag aaatgataca gaatggttaa aacatacgct
      361 ttatttttcc gatgatagaa tagcctatag acctgtcaat atgaagccta tttcggttga
      421 cgcctttccg cctaaagcga ggacgtattg atgaaatttt cgatttatcg ttttaatccc
      481 gaagttgata aaaaacccta tatgcaggac tatgaattag atttaaaaga aacgcctgct
      541 gcaatggtat tggatgcctt aaaattactc aaaaaacaag atgaaacctt atcatttaga
      601 cgttcttgtg gagaaggcgt ttgtggttcg gatggcatga atattaatgg ccgtaatggt
      661 ttggcgtgtg taacaccctt agcgggcatt aaagaaccgg tggtattaag accctttcca
      721 gggatgccag tgattcgaga tctgatagtc gatctgacca atttttataa gcaatatgaa
      781 aaaacaaagc cctatttaca aaatagtcag ccagctcctg gcatcgaacg actacaatcg
      841 ccggcagaac gtgcaaaact cgatgggctg tatgaatgta ttatgtgtgg ctgttgttcg
      901 agtagttgcc catcgtattg gtggaatcct gagaaatttt taggccctgc cgcagcacta
      961 caagctgcgc gatttataga agactccaga gatgatgcaa cattagagcg attagcacaa
     1021 ttggacgatg cctacagtgt ttttcgttgt cgtagcatta tgaattgtgt tgacgtttgt
     1081 cctaaaaacc taaatcccac caaagcgatt gcagatattc gagataaaat tattaaagat
     1141 ggagtgtagt aagtgcagaa attaagtagt tcggtgatta caaaaagagt gtattaatgg
     1201 caaattacaa ggtcacaatg agcgaactgc aaaaaacagc actattagat gctggtaatt
     1261 ctgcgtattt ggaacaactg tacgatgact acttaaaaaa cccggcctct gtttccaccg
     1321 aatggcaaca gtattttgaa aaattacctt tggtagatgg cgttgtctcg gagaccagtc
     1381 acctcgacat ccaagcggca tttaagcaaa tggttcgtca cccaaactgc atcaaagcag
     1441 gcagcataat gccagaagcc agtgggggcg cagtagaaat agcccagctg aaaatcgcat
     1501 tatcccgaga g
//
LOCUS       DAORVT010000286         1509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_101237_length_1509_cov_2.911967, whole genome shotgun
            sequence.
ACCESSION   DAORVT010000286 DAORVT010000000
VERSION     DAORVT010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101237_length_1509_cov_2.911967"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..1474
                     /locus_tag="PK389_05700"
                     /pseudo
     CDS             26..1474
                     /locus_tag="PK389_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965388.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
BASE COUNT          315 a          251 c          376 g          567 t
ORIGIN      
        1 taactactat agatcttagc tcattatgat tccagaaatc ggtaattttt gtttaattct
       61 tgcgcttctt ttagcactta cccaaggaac attgcctctt attggtgcag cgcgaggtat
      121 tccatcctgg atggcgattg ctcgacctgt tgtgcaagga cagttcgttt ttattttttt
      181 ggctttttca tgtttggcct attctttcgt taccagtgat ttttcagtca tgaatgtagc
      241 gagaaattca aattctgaat taccctttca tttccgtctt gctgctactt ggggttctca
      301 tgaaggttcg ttgctgttat gggtgttaat gttagctact tggtcggttg cagtgagtgt
      361 gtttagcaaa caattacccg atgatattgt ggcccgtgtt attggtgttt taggtttgat
      421 cagcgtgggt tttttggcat ttctattatt tacttctaat ccgtttgacc gtctattgcc
      481 agcggctatg gaaggcaacg atttgaatcc attattacaa gacatcggta tggttgtaca
      541 tccgccaatg ttatacatgg gttatgtcgg tttttcaatt gcgtttgcat ttgccattgc
      601 ggcattactg agtggaaagc tggatgcaac ttgggcgcgg tggtcacgtc cttggacgat
      661 tgtggcttgg gttttcttaa caacaggtat tatgatgggt agctggtggg cctattatga
      721 attaggttgg ggtggttggt ggttctggga tccagtagaa aatgcatcgt ttatgccatg
      781 gcttgtaggt actgcactgt tacattcatt ggctgtgacc gaaaaaaggg gaagctttaa
      841 aagctggacg gtattattag cgatctgtgc ctttggatta agtttactgg gcacatttct
      901 ggttcgttca ggtgttttga cttcagtcca tgcttttgca acagatcctg cacgtggtat
      961 ttttattctt gtatttttag tagttgttat aagtgcatca ttgatattgt ttgcctggcg
     1021 tgcgccaaaa gttggaatgg gtggaaagtt tgacttgctt tctcgtgaat cgttattgct
     1081 ggctaataac ttgttacttt tagtggcggc aggtagcgtt ctattgggta cgctgtatcc
     1141 tttaattatt gatgcattgg gtttggggaa attatcggtt ggccctcctt attttgaagc
     1201 ggtgtttgtg cccgttatga caccagcgat ttttttgatt ggtattggtc caatcgctcg
     1261 ctggaaacaa atgagtttac cgactttagt gattcggtta cgttgggcct tcgttgtaag
     1321 cgtcgtttcc gctttgatta cgccttatct catgggtgaa tggaagccat tagtcagttt
     1381 ttgtatgttg ctagcgtttt ggataatcgt gtgtgtagcg gttaatttaa agcaccgtat
     1441 aagcaatagc ggtcaaggta atattttttc taagttttgt atcgggaagt aaagtaaatt
     1501 ttttttaca
//
LOCUS       DAORVT010000287         1500 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5232
            NODE_102320_length_1500_cov_4.199308, whole genome shotgun
            sequence.
ACCESSION   DAORVT010000287 DAORVT010000000
VERSION     DAORVT010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867449
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:11:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,143
            CDSs (total)                      :: 1,125
            Genes (coding)                    :: 1,100
            CDSs (with protein)               :: 1,100
            Genes (RNA)                       :: 18
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 6 of 25
            Pseudo Genes (incomplete)         :: 19 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102320_length_1500_cov_4.199308"
                     /isolate="AMR_MDS_5232"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..371
                     /locus_tag="PK389_05705"
                     /pseudo
     CDS             27..371
                     /locus_tag="PK389_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
     gene            561..1262
                     /locus_tag="PK389_05710"
     CDS             561..1262
                     /locus_tag="PK389_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQY23225.1"
                     /translation="MQEITFGYSKLDPPLIQNFSLSLKKGGHVAIVGATGSGKSTLAK
                     LLSGLYKPWSGSIAIAETPLESLSPQALAENLALVDQDILLFEGSIRDNLTLWNANLP
                     LQELERASKEACIYDVLVNRPKLFDSFVEEGGTNFSSGECQRLEIARALVRNPQIVIL
                     DEGTAALDSLTEQAIIENLKARGYSLLMITHRLSTIRDCDEIIVLTHGQISERGTHEE
                     LMHLKGQYATLVNAS"
     gene            1274..>1500
                     /locus_tag="PK389_05715"
     CDS             1274..>1500
                     /locus_tag="PK389_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY23226.1"
                     /translation="MDKLVDKGILPTDSGQLEIMDESLIELLSIFEPEKTEDLIHIQS
                     QNNNGLNREDALFSACSKVAQVLGISLVKPN"
BASE COUNT          485 a          303 c          282 g          430 t
ORIGIN      
        1 accactagga cttctttgct gtcgtttgca cgattctcta ttttatttca aaccgccttc
       61 gtgatgtttc tcgaaaattt ttacaagaca gaggaaaatt agcaggcatc gaaatcaatg
      121 ggatcaaaac catagaaacc ctaaaatctg cagctttgga tgctgttttt tttaaacgat
      181 ggtctggaaa tcatgcgaaa attatcgact cacaacagaa gttatcttta tattaccaac
      241 taatttcgac aacatccagc ctgctatccg gtctatccat gatttttata ttaggtcttg
      301 gtagttatcg gataatggaa ggatttttaa ccgttggtac actgatcgct tttcagactc
      361 ttttatccag ctttaatgac cccctcaata cactactggg tattggtaca aaattacaag
      421 aagtaccagg agatattgcg cgtttagaag atgtcttagc acaagtagaa gatacacgtt
      481 ttacaaatat accttccaca gaacaatcca ttcaagcaga aataaaaacg acatccttgg
      541 aacacaacat gagtatcatt atgcaagaga taacctttgg gtattcaaag ctcgatcctc
      601 cccttatcca aaactttagt ttatctctta aaaaaggcgg acatgtcgca atcgtaggcg
      661 caacaggcag cgggaaatca acgcttgcaa aattgctcag cggtctttat aaaccctggt
      721 ctggtagtat cgcaatcgca gaaacaccgc tagaatcact ttcgcctcaa gcgttagccg
      781 aaaatcttgc cttagttgat caagatattt tactatttga aggctcaatt cgagataatt
      841 taacattatg gaatgctaat cttccactgc aggaactaga acgcgcctca aaagaagctt
      901 gtatttacga tgttcttgtc aaccgcccca aattgtttga ctcctttgtt gaagaaggag
      961 gtacaaactt tagcagcggg gaatgtcaac gcttagaaat tgcaagagct ttggtacgta
     1021 acccacaaat agtcatatta gatgaaggaa ctgcagcact ggattcgctg acagaacaag
     1081 caatcattga gaacttaaaa gcacgcggat atagtttatt aatgataacg catcgattaa
     1141 gtaccattcg agattgcgat gaaattatcg tactcacgca tggtcaaatt tcggaacgtg
     1201 gaacccatga agaattgatg catcttaaag gacaatatgc aacactggtt aacgcttctt
     1261 aaaaaggatt accatggata agttagttga taaaggaatc ctgcccactg acagcggtca
     1321 acttgagatt atggatgaaa gtcttattga acttttatca atttttgaac ccgaaaaaac
     1381 agaagactta atacacattc aatctcagaa caataatgga ctaaataggg aggacgcctt
     1441 attttcggcc tgttccaagg tcgcgcaagt tttaggcatt tctcttgtta aaccaaattt
//
